CN104644843A - Chinese prickly ash leaf oral liquid and preparation method thereof - Google Patents
Chinese prickly ash leaf oral liquid and preparation method thereof Download PDFInfo
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- CN104644843A CN104644843A CN201510027061.1A CN201510027061A CN104644843A CN 104644843 A CN104644843 A CN 104644843A CN 201510027061 A CN201510027061 A CN 201510027061A CN 104644843 A CN104644843 A CN 104644843A
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- zanthoxyli bungeani
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- folium zanthoxyli
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Abstract
The embodiment of the invention provides Chinese prickly ash leaf oral liquid and a preparation method thereof and relates to the field of preparation of healthy drinks. The Chinese prickly ash leaf oral liquid is prepared from the following ingredients in proportion by weight: main ingredient: 3% of Chinese prickly ash leaf flavonoid antioxidant substance; auxiliary ingredients: 8% of xylitol, 0.03% of Aspartame and 0.015% of menthol; preservative: 0.05% of potassium sorbate; and a solvent: pure water. The oral liquid has the characteristics that the contents of active ingredients are accurate, antioxidation effect is remarkable and market prospect is bright.
Description
Technical field
The present invention relates to health promoting beverage preparation field, particularly relate to a kind of Folium Zanthoxyli Bungeani oral liquid and preparation method thereof.
Background technology
Pericarpium Zanthoxyli is rutaceae.Pericarpium Zanthoxyli children young stem and leaf, as a kind of gourmet food and flavoring agent, is popular in current Vegetable Market.Folium Zanthoxyli Bungeani chemical composition is mainly containing aromatic oil, glucosides, protein, carbohydrate, cellulose, fat, carotene, vitamin B1, vitamin D, mineral element calcium, ferrum, phosphorus, containing 17 seed amino acids, its Glutamic Acid, proline and serine content account for the major part (Deng Zhenyi etc. 2005) of total amino acid content respectively.Having research to prove Folium Zanthoxyli Bungeani children young stem and leaf is that a kind of protein, fat, carbohydrate and dietary fiber content are high, vitamin B2 content is given prominence to, and be rich in required mineral element, aminoacid composition rationally, the desirable vegetable be of high nutritive value, has value of exploiting and utilizing (Peng Huirong and old instruction 2009).And when Folium Zanthoxyli Bungeani of searching for food after Pericarpium Zanthoxyli fruit maturation, little to the Pericarpium Zanthoxyli fruit yield effect in the coming year.Because Folium Zanthoxyli Bungeani all can be plucked throughout the year, Folium Zanthoxyli Bungeani is fully utilized, turns waste into wealth, produce a kind of oral liquid with anti-oxidation function, both can provide solid foundation for the research of natural plant antioxidant, the economic benefit of this crop of Pericarpium Zanthoxyli can be improved again.
Summary of the invention
Embodiments of the invention provide a kind of Folium Zanthoxyli Bungeani oral liquid and preparation method thereof, and this oral liquid active constituent content is clear and definite, antioxidation remarkable, extremely has market prospect.
For achieving the above object, embodiments of the invention adopt following technical scheme:
A kind of Folium Zanthoxyli Bungeani oral liquid, in Folium Zanthoxyli Bungeani oral liquid, each composition accounts for the weight ratio of oral liquid and is:
Major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant;
Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum;
Antiseptic: 0.05% potassium sorbate.
Solvent: pure water.
A preparation method for Folium Zanthoxyli Bungeani oral liquid, comprising:
Prepare Folium Zanthoxyli Bungeani flavonoid antioxidant: Folium Zanthoxyli Bungeani dries in the shade after adopting, pulverize, cross 60 mesh sieves; With the grain alcohol of 60%, in 350w, the ultrasound bath of 50 DEG C, extract twice, each 15min, solid-liquid ratio is 1: 15, merges extracted twice gained supernatant, removes ethanol, obtain Folium Zanthoxyli Bungeani flavone gruff bring up substance; By described crude extract through purification with macroreticular resin, technique is: 5BV contains the sample liquid of saturated NaCl, its flavone mass concentration is 3.10mg Quercetin/mL, pH=4, with the flow velocity of 2BV/h by food stage D101 resin column (Tianjin Ourui Biology Technology Co., Ltd.); With the grain alcohol of 5BV 70% with desorbing flow velocity 2BV/h eluting; Collect eluent, removing ethanol, obtains Folium Zanthoxyli Bungeani flavonoid antioxidant;
By major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant; Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum; Be dissolved in pure water, ultrasonic accelerate dissolution is placed on 12h in 4 DEG C of refrigerators, and 4000 leave heart 5min, get supernatant, add 0.05% potassium sorbate, and be packaged in cillin bottle with capping machine 10ml/ bottle, water proof boils 30min, obtains Folium Zanthoxyli Bungeani oral liquid.
The present inventor finds that Folium Zanthoxyli Bungeani has extremely strong antioxidant activity in an experiment.Under tracking activity is separated, from Folium Zanthoxyli Bungeani, isolation identification has gone out 9 kinds of Flavonoid substances.Spectrum effect analysis result shows, Quercitroside, hyperin, rutin and afzclin are topmost antioxidant in Folium Zanthoxyli Bungeani.Therefore, the present invention for main component, prepares that a kind of active constituent content is clear and definite, the significant oral liquid of antioxidation with these 4 kinds of Flavonoid substances by said method, extremely will have market prospect.Both can provide solid foundation for the research of natural plant antioxidant, the economic benefit of this crop of Pericarpium Zanthoxyli can be improved again.
Detailed description of the invention
Be clearly and completely described the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
A kind of Folium Zanthoxyli Bungeani oral liquid, in Folium Zanthoxyli Bungeani oral liquid, each composition accounts for the weight ratio of oral liquid and is:
Major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant;
Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum;
Antiseptic: 0.05% potassium sorbate.
Solvent: pure water.
A preparation method for Folium Zanthoxyli Bungeani oral liquid, comprising:
Prepare Folium Zanthoxyli Bungeani flavonoid antioxidant: Folium Zanthoxyli Bungeani dries in the shade after adopting, pulverize, cross 60 mesh sieves; With the grain alcohol of 60%, in 350w, the ultrasound bath of 50 DEG C, extract twice, each 15min, solid-liquid ratio is 1: 15, merges extracted twice gained supernatant, removes ethanol, obtain Folium Zanthoxyli Bungeani flavone gruff bring up substance; By described crude extract through purification with macroreticular resin, technique is: 5BV contains the sample liquid of saturated NaCl, its flavone mass concentration is 3.10mg Quercetin/mL, pH=4, with the flow velocity of 2BV/h by food stage D101 resin column (Tianjin Ourui Biology Technology Co., Ltd.); With the grain alcohol of 5BV 70% with desorbing flow velocity 2BV/h eluting; Collect eluent, removing ethanol, obtains Folium Zanthoxyli Bungeani flavonoid antioxidant;
By major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant; Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum; Be dissolved in pure water, ultrasonic accelerate dissolution is placed on 12h in 4 DEG C of refrigerators, and 4000 leave heart 5min, get supernatant, add 0.05% potassium sorbate, and be packaged in cillin bottle with capping machine 10ml/ bottle, water proof boils 30min, obtains Folium Zanthoxyli Bungeani oral liquid.
Oral liquid finished product provided by the invention is rufous liquid, and have fragrance specific to Folium Zanthoxyli Bungeani, sugariness is moderate, micro-fiber crops, clarifies or has a little precipitation.
The active constituent content of oral liquid provided by the invention and antioxidant activity thereof are detected, its result is as shown in table 1, content (adopting RP-HPLC method to measure) all very high (rutin=2.20 ± 0.07mg/ml of 4 kinds of flavonoid monomer compound in oral liquid, hyperin=1.99 ± 0.07mg/ml, Quercitroside=2.60 ± 0.06mg/ml, afzclin=0.191 ± 0.006mg/ml), total phenols (adopting Forint phenol method to measure) and total flavones (adopting SBC method to measure) content also very high (total phenols=41.36 ± 0.26umol gallic acid/ml, total flavones=55.47 ± 0.15umol quercetin/ml), antioxidant activity is very strong (ABTS value=60.32 ± 0.23 μm ol Trolox/ml also, FRAP value=17.26 ± 0.14 μm olTrolox/ml, DPPH value=34.17 ± 0.05 μm ol Trolox/ml).
The active constituent content of table 1. Folium Zanthoxyli Bungeani oral liquid and antioxidant activity
According to the requirement of GB GB 16740-1997, microbial limit in oral disposition liquid and heavy metal limit check, result is as shown in table 2, total plate count in oral liquid, mycete and yeast, coliform and food-borne pathogens (staphylococcus aureus, Salmonella, shigella, Hemolytic streptococcus) all do not detect, heavy metal limit (lead does not detect, arsenic=0.0029mg/l and hydrargyrum=0.0025mg/l) also meets national requirements.
The evaluation of hygiene of table 2. Folium Zanthoxyli Bungeani oral liquid
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with the protection domain of claim.
Claims (2)
1. a Folium Zanthoxyli Bungeani oral liquid, is characterized in that, in Folium Zanthoxyli Bungeani oral liquid, each composition accounts for the weight ratio of oral liquid and is:
Major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant;
Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum;
Antiseptic: 0.05% potassium sorbate;
Solvent: pure water.
2. a preparation method for Folium Zanthoxyli Bungeani oral liquid, is characterized in that, comprising:
Prepare Folium Zanthoxyli Bungeani flavonoid antioxidant: Folium Zanthoxyli Bungeani dries in the shade after adopting, pulverize, cross 60 mesh sieves; With the grain alcohol of 60%, in 350w, the ultrasound bath of 50 DEG C, extract twice, each 15min, solid-liquid ratio is 1: 15, merges extracted twice gained supernatant, removes ethanol, obtain Folium Zanthoxyli Bungeani flavone gruff bring up substance; By described crude extract through purification with macroreticular resin, technique is: 5BV contains the sample liquid of saturated NaCl, and its flavone mass concentration is 3.10mg Quercetin/mL, pH=4, with the flow velocity of 2BV/h by food stage D101 resin column; With the grain alcohol of 5BV 70% with desorbing flow velocity 2BV/h eluting; Collect eluent, removing ethanol, obtains Folium Zanthoxyli Bungeani flavonoid antioxidant;
By major ingredient: 3% Folium Zanthoxyli Bungeani flavonoid antioxidant; Adjuvant: 8% xylitol, 0.03% aspartame, 0.015% Mentholum; Be dissolved in pure water, ultrasonic accelerate dissolution is placed on 12h in 4 DEG C of refrigerators, and 4000 leave heart 5min, get supernatant, add 0.05% potassium sorbate, and be packaged in cillin bottle with capping machine 10ml/ bottle, water proof boils 30min, obtains Folium Zanthoxyli Bungeani oral liquid.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105919106A (en) * | 2016-05-30 | 2016-09-07 | 新疆源森康乐生物科技有限公司 | Lycium ruthenicum procyanidine oral liquid and preparation method thereof |
| CN109259018A (en) * | 2018-10-24 | 2019-01-25 | 中国科学院兰州化学物理研究所 | The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf |
| CN111418745A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash-Jinlumei leaf composite beverage and preparation method thereof |
| CN111418744A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash leaf beverage and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389449A (en) * | 2011-11-22 | 2012-03-28 | 昆明振华制药厂有限公司 | Erigeron breviscapus extract and preparation method and application thereof |
-
2015
- 2015-01-16 CN CN201510027061.1A patent/CN104644843A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389449A (en) * | 2011-11-22 | 2012-03-28 | 昆明振华制药厂有限公司 | Erigeron breviscapus extract and preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 徐怀德等: "大孔吸附树脂分离纯化花椒叶总黄酮及其产品抗氧化功能研究", 《食品科学》 * |
| 杨立琛等: "大孔吸附树脂纯化花椒叶总黄酮的研究", 《中国调味品》 * |
| 胡晋红: "《实用医院药学》", 30 November 2007 * |
| 范菁华等: "超声波辅助提取花椒叶总黄酮及其体外抗氧化性研究", 《中国食品学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105919106A (en) * | 2016-05-30 | 2016-09-07 | 新疆源森康乐生物科技有限公司 | Lycium ruthenicum procyanidine oral liquid and preparation method thereof |
| CN109259018A (en) * | 2018-10-24 | 2019-01-25 | 中国科学院兰州化学物理研究所 | The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf |
| CN111418745A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash-Jinlumei leaf composite beverage and preparation method thereof |
| CN111418744A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash leaf beverage and preparation method thereof |
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Application publication date: 20150527 |