CN110327395A - Application of the Chinese prickly ash leaf total flavone extract in anti anoxia - Google Patents
Application of the Chinese prickly ash leaf total flavone extract in anti anoxia Download PDFInfo
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- CN110327395A CN110327395A CN201910662123.4A CN201910662123A CN110327395A CN 110327395 A CN110327395 A CN 110327395A CN 201910662123 A CN201910662123 A CN 201910662123A CN 110327395 A CN110327395 A CN 110327395A
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- prickly ash
- chinese prickly
- flavone extract
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- total flavone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/758—Zanthoxylum, e.g. pricklyash
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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Abstract
The present invention provides a kind of Chinese prickly ash leaf total flavone extract with Substituted phenyl-lactic acid.Shown by Clonal Rat Pheochromocytoma tumor PC12 cell experiment; Chinese prickly ash leaf total flavone extract has protective effect to PC12 cellular damage caused by anoxic; with the cell viability improved under anoxia condition; mitigate brain tissue impairment caused by anoxic; with apparent Substituted phenyl-lactic acid; and the reduction of cell viability under anoxia condition can also be significantly reversed, therefore, it can be used as preparation of the active material for anti-anoxic medicine.
Description
Technical field
The present invention relates to a kind of application of Chinese prickly ash leaf total flavone extract in anti anoxia, belong to food and medicine health care neck
Domain.
Background technique
Anoxic refers to because the oxygen supply of tissue is insufficient or with oxygen obstacle, and leads to metabolism, function and the form knot of tissue
Structure is abnormal the pathologic process of variation.Anoxic can lead to tissue damage, participate in the progress and deterioration of a variety of diseases, such as the heart
Stalk and cerebral infarction.Therefore, physically (such as slight hypoxia pretreatment) or chemical (medicament administration) finds effective prevention for prevention
Anoxic related disease is most important.Plant extracts and monomeric compound have protective effect, such as Mongolian oak in prevention anoxia-induced apoptosis
Pi Su (for chromocor compound) and rhodioloside are Typical Representatives therein.It is reported that Quercetin passes through anti-oxidant and anti-apoptotic
Mechanism protects hippocampal neuron to damage from hypobaric hypoxia.Rhodioloside has the protective effect for resisting anoxic to body or cell.
It improves the plateau pneumochysis of hypoxia mice induction, alleviates ischemic brain damage.Therefore, screening has anti-lack from plant
The extract or monomeric compound of oxygen activity are research and development anti-anoxic medicine and the effective method of health care product.
Bunge pricklyash leaf be rutaceae Chinese prickly ash (Zanthoxylum bungeanum) leaf, record it in " Chinese Pharmacopoeia "
Nature and flavor are pungent, hot, nontoxic, and bunge pricklyash leaf can control cold product, choleraic myospasm, tinea pedis, dermatitis rhus, scabies, anti-corrosion, sterilization, desinsection etc., mainly
The ground such as Shaanxi, Gansu, Sichuan, Guizhou, Hebei are distributed in, whole year can harvest, abundance.Chinese prickly ash leaf total flavone extract is
The isolated flavone compound from the agricultural wastes bunge pricklyash leaf that Chinese prickly ash production generates has anti-oxidant, anticancer is anti-to swell
The effects of tumor endocrine metabolic diseases, resisting cardiovascular disease, immunological regulation, inhibitory anti-virus, reducing blood sugar and blood fat.But at present
And have no report about Chinese prickly ash leaf total flavone extract in terms of anti anoxia.
Summary of the invention
The object of the present invention is to provide a kind of Chinese prickly ash leaf total flavone extracts in the application for preparing anti-anoxic medicine, can be used for
Ischemic disease is prevented and treated, to increase its application field.
One, the preparation of Chinese prickly ash leaf total flavone extract
The preparation method of Chinese prickly ash leaf total flavone extract (has health care function referring to CN 201811244878.4 in a kind of bunge pricklyash leaf
The Preparation method and use of the extractive of general flavone of energy), specifically includes the following steps:
(1) bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness 0.1-5mm；
(2) smashed bunge pricklyash leaf is extracted with Extraction solvent, the mass ratio of the bunge pricklyash leaf and Extraction solvent is 1:3-
20, filtering, merging filtrate are extracted, then be concentrated, has obtained general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract is sufficiently dissolved with water, crosses large pore resin absorption column.It is whole with the aqueous solution of crude extract
Be added column in after, standing adsorption for 24 hours, later in order respectively with the pure water of 3.5 times of BV, 30% methanol, 60% methanol, 85% methanol
It is eluted with methanol, each section of eluent is concentrated into thick paste respectively, is transferred in beaker, and labelled, spare, each section is numbered respectively
Are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E(ethyl alcohol).Each section of eluent is concentrated into paste respectively
Elution medicinal extract, Chinese prickly ash leaf total flavone extract as with health care function；
(4) using the general flavone compounds content in Sodium nitrite-aluminium nitrite method measurement Chinese prickly ash leaf extract.C portion elution leaching
General flavone volume parts >=40% of Chinese prickly ash extractive of general flavone in cream.
Two, the Substituted phenyl-lactic acid of Chinese prickly ash leaf total flavone extract
1, cell culture
Clonal Rat Pheochromocytoma tumor PC12 cell strain derives from a kind of transplantable mouse pheochromocytoma, and the cell is to nerve
Growth factor (NGF) has the profiling reaction of reversible neuron, has been widely used for reaction of the research neuron to stimulation.PC12 is thin
Born of the same parents are incubated at the RPMI-1640 culture solution containing 10% fetal calf serum, 100U/ml penicillin and 100 μ g/ml streptomysins, and (Gibco is public
Department) in, in 5%CO2, cultivate under the conditions of 37 DEG C of saturated humidities, logarithmic growth phase cell is tested.
The detection of 2, cell viabilities
Using cell Proliferation/toxicity test (Cell Counting Kit-8, CCK-8) kit (Japanese Dojindo company)
It is horizontal to detect cell viability.Chinese prickly ash leaf total flavone extract is formulated as 0.03 mg/mL, 0.1 mg/mL and 0.3 mg/mL tri-
A various dose group, logarithmic growth phase PC12 cell are divided into normal group cell and anoxic group cell, and every group of cell, which has, is divided into control
Group and drug-treated group, are inoculated in 96 orifice plates, 4000, every hole cell.
The extract of various dose is added in drug-treated group hole, after 6 hours, anoxic group cell is placed in O2Concentration is
24 hours in 0.1% anoxic case.Later, 10 μ L CCK-8 solution are added to every hole, culture plate is placed in incubator and is incubated for
Then 2h measures the absorbance (OD450) at 450nm with microplate reader.The vigor of cellular control unit is set as 100%, is calculated
The relative percentages of each processing group cell viability.
3. experimental result
Fig. 1 is protective effect figure of the Chinese prickly ash leaf flavone extract to PC12 cellular damage caused by anoxic.Chinese prickly ash leaf flavonoids mention
After taking object to handle PC12 cell 6h, in oxygen concentration for for 24 hours, cell survival rate is tested with CCK8 and tested under 0.1% environment.Experiment
Become Mean+SD；* P < 0.01 P < 0.05, *.As shown in Fig. 1, PC12 cell is placed in O2The anoxic case that concentration is 0.1%
In for 24 hours after, cell viability significantly reduces, only the 50% of control group.But the Chinese prickly ash leaf flavonoids that various dose is added extract
Object pre-processes the PC12 cell of 6h, vigor has a different degrees of rising, especially 0.1mg/mL and 0.3mg/mL compared with
Under high dose, and it can also significantly reverse the reduction of cell viability under anoxia condition.Therefore, extractive of general flavone has to improve and lack
Cell viability under the conditions of oxygen mitigates brain tissue impairment caused by high altitude anoxia, can be used as active material for anti-anoxic medicine
Preparation.
Three, the preparation of anti-anoxic medicine
Using Chinese prickly ash leaf flavone extract as active constituent, by with pharmacological or physiological acceptable auxiliary material and conventional Chinese medicine system
Oral preparations or injection is made in the preparation process of agent.
In Chinese prickly ash leaf flavone extract, general flavone content >=40%.
Oral preparations can be powder, granule, capsule, soft capsule, pulvis, pill, tablet, oral solution.
Granules medicine prepared by the present invention, by carrying out clinical observations to twenties high altitude anoxia patients, as a result table
Bright, Chinese materia medica preparation of the invention has a good therapeutic effect for high altitude anoxia, and total effective rate is up to 100%.
Detailed description of the invention
Fig. 1 is protective effect figure of the Chinese prickly ash leaf flavone extract to PC12 cellular damage caused by anoxic.
Specific embodiment
The application that anti anoxia is wanted is prepared to Chinese prickly ash leaf flavone extract below by specific embodiment to be described further.
The preparation of embodiment 1, tablet
Active component: Chinese prickly ash leaf flavone extract, general flavone content 50%；
Preparation process: taking active component 12g, and microcrystalline cellulose 112.5g is added and is uniformly mixed, with mass concentration be 0.8% it is hard
Fatty acid magnesium is lubricant, and 5% sodium carboxymethyl starch is disintegrating agent, is made tablet using direct compression method, every 250mg,
It is made 100；
Usage and dosage: 3 times a day, each 2-4 piece is eaten after meal.
The preparation of embodiment 2, granule
Active component: Chinese prickly ash leaf flavone extract, general flavone content 75%；
Preparation process: taking 8 g of active component, and starch 192g is added and is uniformly mixed, and is with the starch slurry that mass concentration is 10 ~ 12%
Adhesive, wet granulation are crossed 12 ~ 14 meshes, are dried under reduced pressure at 65 ~ 75 DEG C, whole grain, dispense, every bag of 10 g, are made 20
Bag；
Usage and dosage: it 3 times a day, 1 bag every time, eats after meal.
The preparation of embodiment 3, oral solution
Active component: Chinese prickly ash leaf flavone extract, general flavone content 88%；
Preparation process: taking active component 12g, adds appropriate food additives (white granulated sugar, potassium sorbate), dissolves by formula rate
It is mixed to prepare 1000 mL, sterilizing, dispenses 100 bottles obtained, every bottle of 10 mL；
Usage and dosage: it is 3 times a day, 1-2 bottles each, it eats after meal.
Claims (4)
1. Chinese prickly ash leaf total flavone extract is applied in preparing anti-anoxic medicine.
2. Chinese prickly ash leaf total flavone extract as described in claim 1 is applied in preparing anti-anoxic medicine, it is characterised in that: with flower
Green pepper leaf flavone extract is active constituent, by with the preparation of pharmacological or physiological acceptable auxiliary material and conventional Chinese materia medica preparation
Oral preparations or injection is made in technique.
3. Chinese prickly ash leaf total flavone extract as claimed in claim 1 or 2 is applied in preparing anti-anoxic medicine, it is characterised in that:
In Chinese prickly ash leaf flavone extract, general flavone content >=40%.
4. Chinese prickly ash leaf total flavone extract as claimed in claim 2 is applied in preparing anti-anoxic medicine, it is characterised in that: for oral administration
Preparation is powder, granule, capsule, soft capsule, pulvis, pill, tablet, oral solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910662123.4A CN110327395A (en) | 2019-07-22 | 2019-07-22 | Application of the Chinese prickly ash leaf total flavone extract in anti anoxia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910662123.4A CN110327395A (en) | 2019-07-22 | 2019-07-22 | Application of the Chinese prickly ash leaf total flavone extract in anti anoxia |
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| CN110327395A true CN110327395A (en) | 2019-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910662123.4A Pending CN110327395A (en) | 2019-07-22 | 2019-07-22 | Application of the Chinese prickly ash leaf total flavone extract in anti anoxia |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109259018A (en) * | 2018-10-24 | 2019-01-25 | 中国科学院兰州化学物理研究所 | The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf |
-
2019
- 2019-07-22 CN CN201910662123.4A patent/CN110327395A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109259018A (en) * | 2018-10-24 | 2019-01-25 | 中国科学院兰州化学物理研究所 | The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf |
Non-Patent Citations (2)
| Title |
|---|
| KAI ZHONG等: "Antioxidant and Cytoprotective Activities of Flavonoid", 《JOURNAL OF FOOD AND NUTRITION RESEARCH》 * |
| 张任权等: "N-乙酰半胱氨酸保护PC12细胞对抗化学性低氧诱导的损伤", 《解剖学研究》 * |
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