CN109258526A - A kind of plasma irradiation breeding method of crucian - Google Patents
A kind of plasma irradiation breeding method of crucian Download PDFInfo
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- CN109258526A CN109258526A CN201811451693.0A CN201811451693A CN109258526A CN 109258526 A CN109258526 A CN 109258526A CN 201811451693 A CN201811451693 A CN 201811451693A CN 109258526 A CN109258526 A CN 109258526A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to crucian breeding fields, the plasma that the fast crucian of superior progeny, the speed of growth can be obtained more particularly to one kind irradiates breeding method, select artificial induced spawning after parent, fertilized eggs are adhered to fixed attached ovum on piece, with pallet in instrument illumination window lower slider, fertilized eggs room temperature atmospheric plasma is irradiated, the attached ovum piece of fertilized eggs after irradiation, which is put on, is put in culture pond cultivation, attached ovum piece after irradiation with fertilized eggs, which is fixed in cement hatchery, hatches, soya-bean milk is sprayed after three days, as fish body is grown up, it is gradually transitions and is fed with pellet；The present invention improves the speed of growth of carp, shortens the culture-cycle, improves income.
Description
Technical field
The present invention relates to crucian breeding fields, and in particular to one kind can obtain the fast crucian of superior progeny, the speed of growth
Plasma irradiate breeding method.
Background technique
Genetics-breeding in fish in aquaculture industry is always one of the emphasis of aquatic science research field.Norway loses
It passes scholar Dr. Gjedrem Trygve and points out the world aquaculture product breed variety from genetic improvement that there was only 1-2%.
And China's aquaculture kind reaches more than 150, only 10% breed variety passes through genetic improvement, and 16.5% from cultivation improvement
New varieties obtain, aquaculture genetic improvement rate 17%, breeding coverage rate 50%, so, excellent novel species improvement breeding and new varieties
Acquisition, still have huge room for promotion.Fish breeding is common to generally use unexpected mass incident method, family selective breeding, parent's breeding
With comprehensive breeding etc., but in the prevalence of breeding cycle, long, manpower and material resources put into big, constrain the fast development of fish breeding,
So advanced breeding technique is taken, the efficiency and seedling established new breeding research innovation platform, improve aquatic products genetic breeding
Quality is of great significance to the sound development for promoting culture fishery.
Crucian is one of high-quality aquatic products, it is full of nutrition, delicious flavour.But since the crucian speed of growth is slow, some ground
Square kind two is older less than 200 grams, therefore not by culturist as main cultivation object.
Existing crucian breeding is all natural propagation, and offspring's growth performance, anti-adversity are easy to degenerate.Offspring's speed of growth
Slowly, 1 age 1-2 can grow to 50 grams or so, 2 150-200 grams of ages.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art and superior progeny, life can be obtained by providing one kind
The plasma of long fireballing crucian irradiates breeding method.
The technical scheme of the present invention is realized as follows:
A kind of plasma irradiation breeding method of crucian, comprising the following steps:
Step 1), to select mature red crucian in parent fish rearing pond be parent；
Step 2), raun per kilogram inject oxytocic hormone LRH_A50-100ug+HCG800-1000IU, milter halves, primary to infuse
It penetrates；
Step 3), fertilized eggs are adhered to fixed attached ovum on piece, with pallet in instrument illumination window lower slider, fertilized eggs are used
The irradiation of room temperature atmospheric plasma, exposure dose 400-800W, irradiation time are divided into 3-8 minutes, irradiation distance 1-3mm, after irradiation
The attached ovum piece of fertilized eggs put on be put in culture pond cultivation.
Step 4), the attached ovum piece for having fertilized eggs after irradiation is fixed in cement hatchery and hatches, spray beans after three days
Slurry, as fish body is grown up, is gradually transitions and is fed with pellet；
Step 5), monthly its upgrowth situation is measured, so as to study its merit performance situation.
By step 4) after same parent there are 8 kinds of phenotypes, it may be assumed that it is green black short-tail, the big bifurcated of green black big tail, green black
The big small bifurcated of tail, green black big tail not bifurcated, the big tripe of green black big tail microcephaly, red and black, pure red, red white.
The positive effect of the present invention is: ARTP(Atmospheric and Room Temperature Plasma) mutagenesis
Breeding technique is that the active particle in plasma is acted on organism, changes biological cell wall/film structure and permeability
Become, and further cause nucleic acid damaging, eventually lead to the new approaches of physical mutagenesis that can stablize heredity, be develop in recent years it is extremely rapid
Biology Breeding new technology.Compared with other method of mutagenesis, ARTP mutagenesis mutation rate is high, and Phenotypic Diversity is abundant, genetic stability
Well, the advantages that no poisonous and harmful substance remains, as the breeding method of NO-GMO a kind of, ARTP is widely used in microorganism, plants
Object, animal genetic breeding field.
Room temperature atmospheric plasma irradiates the fish breeding of mutagenesis crucian, and eight kinds of phenotypes, five kinds of main phenotypes: two occurs in offspring
Kind is suitable for developing ornamental fish, and body colour is very gorgeous；Two kinds are suitable for developing commodity fish culture, and the speed of growth is fast, general place crucian
It growing to 200 grams within kind 2 years, can list, 1 age of fish of mutation breeding of the present invention, two ages were more than 500 grams just up to 200 grams or more,
Current year average individual weighs 230.5 grams, and maximum 419 grams of individual has obvious growth vigor, Parents has been used as to cultivate.
Irradiation breeding principle of the invention is: plasma is rich in active particle, and active particle can be penetrated effectively carefully
Cell wall cell membrane is applied into the cell, and active particle can generate apparent destruction to DNA material, and DNA is damaged thin
Born of the same parents actively start SOS repair mechanism, generate a large amount of mutant strain, by breeding, can obtain desired breeding, function admirable
Kind can significantly improve yield, obtain benefit.
Conventional ion beam irradiates mutagenesis, is unsuitable for fish ovum, sperm and fertilized eggs and uses, because it is in high temperature and pressure
Lower completion, therefore several seconds can kill sperm, ovum and fertilized eggs.And test method used now and solve this technology,
It is to be completed under atmospheric pressure at room, under the premise of suitable dose is set, mutagenesis will not be had an impact because of pressure and high temperature.
Detailed description of the invention
Fig. 1 is mutagenesis crucian changes of weight tendency chart of the invention.
Fig. 2 is mutagenesis crucian carp fish length trend chart of the invention.
Fig. 3 is the mutagenesis crucian speed of growth and other crucian comparison diagrams of the invention.
Specific embodiment
As shown in Fig. 1 to 3, a kind of plasma irradiation breeding method of crucian, comprising the following steps:
Step 1), to select mature red crucian in parent fish rearing pond be parent；
Step 2), raun per kilogram inject oxytocic hormone LRH_A50-100ug+HCG800-1000IU, milter halves, primary to infuse
It penetrates；
Step 3), fertilized eggs are adhered to fixed attached ovum on piece, with pallet in instrument illumination window lower slider, fertilized eggs are used
The irradiation of room temperature atmospheric plasma, exposure dose 400-800W, irradiation time are divided into 3-8 minutes, irradiation distance 1-3mm, after irradiation
The attached ovum piece of fertilized eggs put on be put in culture pond cultivation, wherein room temperature refers to 30-35 DEG C of jet temperature, and normal pressure refers to that a standard is big
Air pressure.
Step 4), the attached ovum piece for having fertilized eggs after irradiation is fixed in cement hatchery and hatches, spray beans after three days
Slurry, as fish body is grown up, is gradually transitions and is fed with pellet；
Step 5), monthly its upgrowth situation is measured, so as to study its merit performance situation.
By step 4) after same parent there are 8 kinds of phenotype, eight kinds of phenotypes and be respectively as follows: green black short-tail, green black big
The big bifurcated of tail, the small bifurcated of green black big tail, green black big tail not bifurcated, the big tripe of green black big tail microcephaly, red and black, pure red, red white.
The present invention tests red crucian, obtains fine experimental result, and the measurement result of mutagenesis 1 age, 60 tail for generations is shown in
The following table 1:
The red crucian plasma mutagenesis of table 1 phenotype measurement result for generations
As can be seen from the table, clearly, especially green black short-tail most has commodity fish market to open to the advantage shown after mutagenesis
It makes an offer value, its growth is most fast, accounting highest.
Claims (2)
1. a kind of plasma of crucian irradiates breeding method, which comprises the following steps:
Step 1), to select mature red crucian in parent fish rearing pond be parent；
Step 2), raun per kilogram inject oxytocic hormone LRH_A50-100ug+HCG800-1000IU, milter halves, primary to infuse
It penetrates；
Step 3), fertilized eggs are adhered to fixed attached ovum on piece, with pallet in instrument illumination window lower slider, fertilized eggs are used
The irradiation of room temperature atmospheric plasma, exposure dose 400-800W, irradiation time are divided into 3-8 minutes, and irradiation distance is 1-3 ㎜, irradiation
The attached ovum piece of fertilized eggs afterwards, which is put on, is put in culture pond cultivation；
Step 4), the attached ovum piece for having fertilized eggs after irradiation is fixed in cement hatchery and hatches, spray soya-bean milk after three days,
As fish body is grown up, it is gradually transitions and is fed with pellet；
Step 5), monthly measure a upgrowth situation, in favor of research irradiation after merit phenotypic analysis.
2. the plasma irradiating breeding method of crucian according to claim 1, it is characterised in that: by step 4) after
Same parent there are 8 kinds of phenotypes, 8 kinds of phenotypes are respectively: green black short-tail, the big bifurcated of green black big tail, green black small point of big tail
Fork, green black big tail not bifurcated, the big tripe of green black big tail microcephaly, red and black, pure red, red white.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811451693.0A CN109258526A (en) | 2018-11-30 | 2018-11-30 | A kind of plasma irradiation breeding method of crucian |
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| CN201811451693.0A CN109258526A (en) | 2018-11-30 | 2018-11-30 | A kind of plasma irradiation breeding method of crucian |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115104559A (en) * | 2022-07-22 | 2022-09-27 | 福建省水产研究所（福建水产病害防治中心） | Method for mutagenizing black male clown fish body color to become red by ARTP (ARTP) |
| CN117502338A (en) * | 2023-12-22 | 2024-02-06 | 中国水产科学研究院黄海水产研究所 | Method for plasma mutagenesis of fertilized eggs of prawns at room temperature under normal pressure |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11279197A (en) * | 1998-02-26 | 1999-10-12 | Council Scient Ind Res | Purified new goat epididymal advancing protein and purification of the protein useful as fertility promoting factor/suppressing factor |
| CN101940184A (en) * | 2010-09-21 | 2011-01-12 | 华南农业大学 | Breeding and culturing method of improved crucian carp |
| CN103039388A (en) * | 2012-09-14 | 2013-04-17 | 吴江市水产养殖有限公司 | Fish radiation breeding method |
| CN103179771A (en) * | 2013-02-26 | 2013-06-26 | 大连民族学院 | Atmospheric low-temperature plasma generating device and application thereof |
| CN203700376U (en) * | 2013-11-12 | 2014-07-09 | 无锡思清源生物科技有限公司 | Atmospheric and room temperature plasma mutation breeding device |
| CN103981091A (en) * | 2014-05-23 | 2014-08-13 | 无锡思清源生物科技有限公司 | Novel normal-pressure room-temperature plasma induced mutation breeding device |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN107201309A (en) * | 2016-03-16 | 2017-09-26 | 洛阳华清天木生物科技有限公司 | A kind of novel plasma mutation breeding device |
| CN107751187A (en) * | 2017-12-05 | 2018-03-06 | 福建师范大学 | A kind of preparation method of the inactivation sperm liquid of inducible fish gynogenesis |
| CN107787885A (en) * | 2017-12-05 | 2018-03-13 | 福建师范大学 | A kind of body colour character pure line breeding method of Artificial gynogenesis goldfish |
| CN107889772A (en) * | 2017-11-09 | 2018-04-10 | 辽宁机电职业技术学院 | A kind of promotion cultivation fish growing method and device based on plasma irradiation |
-
2018
- 2018-11-30 CN CN201811451693.0A patent/CN109258526A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11279197A (en) * | 1998-02-26 | 1999-10-12 | Council Scient Ind Res | Purified new goat epididymal advancing protein and purification of the protein useful as fertility promoting factor/suppressing factor |
| CN101940184A (en) * | 2010-09-21 | 2011-01-12 | 华南农业大学 | Breeding and culturing method of improved crucian carp |
| CN103039388A (en) * | 2012-09-14 | 2013-04-17 | 吴江市水产养殖有限公司 | Fish radiation breeding method |
| CN103179771A (en) * | 2013-02-26 | 2013-06-26 | 大连民族学院 | Atmospheric low-temperature plasma generating device and application thereof |
| CN203700376U (en) * | 2013-11-12 | 2014-07-09 | 无锡思清源生物科技有限公司 | Atmospheric and room temperature plasma mutation breeding device |
| CN103981091A (en) * | 2014-05-23 | 2014-08-13 | 无锡思清源生物科技有限公司 | Novel normal-pressure room-temperature plasma induced mutation breeding device |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN107201309A (en) * | 2016-03-16 | 2017-09-26 | 洛阳华清天木生物科技有限公司 | A kind of novel plasma mutation breeding device |
| CN107889772A (en) * | 2017-11-09 | 2018-04-10 | 辽宁机电职业技术学院 | A kind of promotion cultivation fish growing method and device based on plasma irradiation |
| CN107751187A (en) * | 2017-12-05 | 2018-03-06 | 福建师范大学 | A kind of preparation method of the inactivation sperm liquid of inducible fish gynogenesis |
| CN107787885A (en) * | 2017-12-05 | 2018-03-13 | 福建师范大学 | A kind of body colour character pure line breeding method of Artificial gynogenesis goldfish |
Non-Patent Citations (1)
| Title |
|---|
| 吴亦楠等: "ARTP生物育种技术与装备研发及其产业化发展 ", 《生物产业技术》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115104559A (en) * | 2022-07-22 | 2022-09-27 | 福建省水产研究所（福建水产病害防治中心） | Method for mutagenizing black male clown fish body color to become red by ARTP (ARTP) |
| CN115104559B (en) * | 2022-07-22 | 2023-10-27 | 福建省水产研究所（福建水产病害防治中心） | Method for changing body color of black princess clown fish into red through ARTP mutagenesis |
| CN117502338A (en) * | 2023-12-22 | 2024-02-06 | 中国水产科学研究院黄海水产研究所 | Method for plasma mutagenesis of fertilized eggs of prawns at room temperature under normal pressure |
| CN117502338B (en) * | 2023-12-22 | 2024-05-07 | 中国水产科学研究院黄海水产研究所 | Method for plasma mutagenesis of fertilized eggs of prawns at room temperature under normal pressure |
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