CN109234198A - A method of utilizing swine manure wastewater, aquaculture wastewater fermented and cultured microorganism - Google Patents
A method of utilizing swine manure wastewater, aquaculture wastewater fermented and cultured microorganism Download PDFInfo
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Abstract
It is specifically a kind of using swine manure wastewater, the method for aquaculture wastewater fermented and cultured microorganism the present invention relates to bio-fermentation engineering field, in particular to culture medium, the method for fermented and cultured microorganism are prepared using swine manure wastewater, aquaculture wastewater.Step 1: the processing of swine manure wastewater, aquaculture wastewater；Step 2: the preparation of culture medium；Step 3: inoculated and cultured.The present invention is using swine manure wastewater, aquaculture wastewater come fermented and cultured microorganism, protein, amino acid, N, P, middle microelement in sewage etc. is made full use of to carry out fermenting and producing, the microorganism of production may be used as feed addictive, microbial pesticide and microbial manure again.So that swine manure wastewater, aquaculture wastewater obtain green, environmental protection recycle.The utilization of wastewater resource that the present invention will generate during livestock and poultry cultivation solves the problems, such as to perplex industry at present, belongs to initiative project, be also of great significance to preserving the ecological environment for cultivating beneficial microbe needed for aquaculture, planting industry.
Description
Technical field
It is specifically a kind of to be sent out using swine manure wastewater, aquaculture wastewater the present invention relates to bio-fermentation engineering field
The method of ferment culture microorganism in particular to prepares culture medium, the micro- life of fermented and cultured using swine manure wastewater, aquaculture wastewater
The method of object.
Background technique
Since the eighties in last century, the aquaculture in China enters quick developing stage, but the animal dung that aquaculture generates
Just sewage, aquaculture wastewater and other sewage are discharged into rivers and lakes, and harmful components once enter in underground water, can cause persistence
Organic contamination, it is extremely difficult to administer, restore.At present swine manure wastewater, aquaculture wastewater processing problem be influence enterprise persistently send out
One significant problem of exhibition.With levying for national environmental protection tax, the increasingly attention environmental problem of national environmental protection policy, animal dung
Just a kind of environmental protection, green processing method are badly in need of in the processing of sewage, aquaculture wastewater.
Contain a large amount of soluble protein, amino acid, N, P, middle microelement etc. in swine manure wastewater, aquaculture wastewater,
These are all substances required for microorganism grows, and are adding a part of maize flour, beans in swine manure wastewater, aquaculture wastewater
The substances such as dregs of rice powder, wheat bran, sugar, or the industrial water of solid medium is replaced with swine manure wastewater, aquaculture wastewater, it can make
The standby production medium produced at microbial fermentation.The microorganism wherein produced includes bacillus subtilis, Su Yun gold gemma bar
Bacterium, bacillus amyloliquefaciens, muscardine, aspergillus oryzae, actinomyces etc. are good feed addictive, microbial pesticide and micro- life again
Object fertilizer.
Summary of the invention
The present invention provides a kind of benefit to solve the problems, such as swine manure wastewater, aquaculture wastewater pollution on the environment
With swine manure wastewater, the method for aquaculture wastewater fermented and cultured microorganism.
The present invention is achieved by the following technical solutions: a kind of to utilize swine manure wastewater, aquaculture wastewater fermented and cultured
The method of microorganism, includes the following steps:
Step 1: the processing of swine manure wastewater, aquaculture wastewater
By livestock and poultry cultivation sewage by separation of solid and liquid, 0.75-0.95 portions of supernatants are taken, it is spare；
Or by feces of livestock and poultry by being separated by solid-liquid separation, placement takes 0.64-0.82 portions of supernatants, spare；
Or feces of livestock and poultry, aquaculture wastewater ferment 20-55 days in methane-generating pit, it is separated by solid-liquid separation, takes 0.66-0.86 portions of supernatants,
It is spare；
Or by feces of livestock and poultry, aquaculture wastewater by being separated by solid-liquid separation, liquid phase is handled 5-20 days, is taken into biochemical sewage processing pond
0.62-0.84 portions of supernatants, it is spare；
Step 2: the preparation of culture medium
The preparation of fluid nutrient medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, add 0.01-
0.08 part of bean cake powder, 0.01-0.06 parts of maize flour, 0.01-0.03 parts of glucose, 0.001-0.005 parts of peptone,
0.001-0.005 parts of yeast powder, 0.0005-0.005 parts of manganese sulfate, 0.0005-0.005 parts of potassium dihydrogen phosphate,
0.0005-0.005 parts of magnesium sulfate, are sufficiently mixed, and pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes；
The preparation of solid medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, are then added to
0.3-1.2 parts of wheat bran, 0.2-0.8 parts of maize flour, 0.2-0.6 parts of bean cake powder, 0.0005-0.005 parts of manganese sulfate,
In 0.0005-0.005 parts of potassium dihydrogen phosphate, 0.0005-0.005 parts of sulfuric acid magnesium compounds, 121 ± 2 DEG C sterilize 120 minutes；
Step 3: inoculated and cultured
By bacillus subtilis, bacillus thuringiensis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus megaterium, silicon
Hydrochlorate bacillus, saccharomycete, rice aspergillus, Trichoderma or Actinomycetes species are inoculated into liquid or solid culture medium；Liquid training
The condition of culture for supporting base is 32-37 DEG C, ventilation ratio 0.5-1.5, and revolving speed is that 60-300 turns/min, is cultivated 20-72 hours, fermentation
Obtain fermentation liquid；The condition of culture of solid medium is 28-32 DEG C, and periodic agitation is cultivated 24-96 hours, obtains solid culture
Object.
In the present invention, the microorganism that can be cultivated includes bacterium, saccharomycete, fungi etc..And the animal dung
Just sewage include but is not limited to the excrement of pig, ox, sheep, chicken, duck by the isolated sewage such as natural subsidence, separation of solid and liquid and
Isolated swine manure wastewater obtains sewage using flocculation, biological treatment, physical and chemical processing etc..The livestock and poultry cultivation is dirty
Water includes but is not limited to pig, ox, sheep, chicken, sewage caused by livestock and poultry farm is rinsed in duck breeding process and rinses livestock and poultry and is supported
It grows sewage caused by field and obtains sewage using flocculation, biological treatment, physical and chemical processing etc..
The strain includes but is not limited to by bacillus subtilis, bacillus thuringiensis, bacillus licheniformis, Xie Dian
Afnyloliquefaciens, bacillus megaterium, silicate bacillus, saccharomycete, rice aspergillus, Trichoderma, actinomyces etc..
The medium component includes but is not limited to swine manure wastewater, aquaculture wastewater, industrial water, maize flour, beans
Dregs of rice powder, glucose, peptone, manganese sulfate etc..
The training method, including the general Submerged liquid culturation tank of fermentation industry, solid fermentation apparatus, according to each bacterium
Kind the culture medium for needing to select suitable growth, when thallus reaches maximum quantity, metabolite reach maximum, maturation is put tank, is received
Collection.
It is obtained invention further provides the method using swine manure wastewater, aquaculture wastewater fermented and cultured microorganism
The effect of fermentation liquid or solid culture in microbial manure, microbial pesticide or feed addictive.
As the further improvement of application technology scheme of the present invention, the fermentation liquid that the fermentation obtains, then add 0-99.99
Part by decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of biphosphate
Potassium, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of phosphoric acid
Monoammonium, 0-50 parts of potassium sulfate, is prepared into microbial manure, microbial pesticide or feed addictive；The solid culture, then
Addition 0-99.99 parts by decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts
Potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-
60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into microbial manure, microbial pesticide or feed addictive.
Further, the fermentation liquid that the fermentation obtains is prepared into living containing 10,000,000,000-5,000 hundred million/g by spray drying
The bacterium powder of bacterium, then add 0-99.99 parts by decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts it is yellow rotten
Sour potassium, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005
Part ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into microbial manure, microbial pesticide or feed
Additive；Cyclonic separation solid fermentation object obtains pure conidia powder, then add 0-99.99 parts by decomposed feces of livestock and poultry or contain
The industrial by-products of organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts it is sweet
Oil, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into
Microbial manure, microbial pesticide or feed addictive.
Compared with prior art, the present invention having the following beneficial effects:
Using swine manure wastewater, aquaculture wastewater come fermented and cultured microorganism, make full use of protein in sewage, amino acid,
N, P, middle microelement etc. carry out fermenting and producing, and the microorganism of production may be used as feed addictive, microbial pesticide and micro- again
Bio-feritlizer.So that swine manure wastewater, aquaculture wastewater obtain green, environmental protection recycle.The present invention is by livestock and poultry cultivation mistake
The utilization of wastewater resource generated in journey solves tired at present for cultivating beneficial microbe needed for aquaculture, planting industry
The problem for disturbing industry belongs to initiative project, is also of great significance to preserving the ecological environment.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
A method of using swine manure wastewater, aquaculture wastewater fermented and cultured microorganism, include the following steps:
Step 1: the processing of swine manure wastewater, aquaculture wastewater
By livestock and poultry cultivation sewage by separation of solid and liquid, 0.75-0.95 portions of supernatants are taken, it is spare；
Or by feces of livestock and poultry by being separated by solid-liquid separation, placement takes 0.64-0.82 portions of supernatants, spare；
Or feces of livestock and poultry, aquaculture wastewater ferment 20-55 days in methane-generating pit, it is separated by solid-liquid separation, takes 0.66-0.86 portions of supernatants,
It is spare；
Or by feces of livestock and poultry, aquaculture wastewater by being separated by solid-liquid separation, liquid phase is handled 5-20 days, is taken into biochemical sewage processing pond
0.62-0.84 portions of supernatants, it is spare；
Step 2: the preparation of culture medium
The preparation of fluid nutrient medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, add 0.01-
0.08 part of bean cake powder, 0.01-0.06 parts of maize flour, 0.01-0.03 parts of glucose, 0.001-0.005 parts of peptone,
0.001-0.005 parts of yeast powder, 0.0005-0.005 parts of manganese sulfate, 0.0005-0.005 parts of potassium dihydrogen phosphate,
0.0005-0.005 parts of magnesium sulfate, are sufficiently mixed, and pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes；
The preparation of solid medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, are then added to
0.3-1.2 parts of wheat bran, 0.2-0.8 parts of maize flour, 0.2-0.6 parts of bean cake powder, 0.0005-0.005 parts of manganese sulfate,
In 0.0005-0.005 parts of potassium dihydrogen phosphate, 0.0005-0.005 parts of sulfuric acid magnesium compounds, 121 ± 2 DEG C sterilize 120 minutes；
Step 3: inoculated and cultured
By bacillus subtilis, bacillus thuringiensis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus megaterium, silicon
Hydrochlorate bacillus, saccharomycete, rice aspergillus, Trichoderma or Actinomycetes species are inoculated into liquid or solid culture medium；Liquid training
The condition of culture for supporting base is 32-37 DEG C, ventilation ratio 0.5-1.5, and revolving speed is that 60-300 turns/min, is cultivated 20-72 hours, fermentation
Obtain fermentation liquid；The condition of culture of solid medium is 28-32 DEG C, and periodic agitation is cultivated 24-96 hours, obtains solid culture
Object.
In the first step of the invention, by livestock and poultry cultivation sewage by being separated by solid-liquid separation, when taking 0.75-0.95 portions of supernatants,
Preferably take 0.82-0.87 parts.By feces of livestock and poultry by being separated by solid-liquid separation, place, when taking 0.64-0.82 portions of supernatants, preferably
Take 0.72-0.78 parts.By swine manure wastewater, aquaculture wastewater when methane-generating pit ferments 20-55 days, preferred fermentation time is taken
It 30-44 days, is separated by solid-liquid separation, when taking 0.66-0.86 portions of supernatants, preferably takes 0.72-0.82 parts.By swine manure wastewater, support
Sewage is grown by being separated by solid-liquid separation, liquid phase is into biochemical sewage processing pond, and when handling 5-20 days, the preferred processing time is 10-15 days,
When taking 0.62-0.84 portions of supernatants, 0.72-0.80 parts are preferably taken.
In second step of the invention, when the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10,
Include the case where not mixing with clear water.
Further, it is obtained the present invention provides the method using swine manure wastewater, aquaculture wastewater fermented and cultured microorganism
Effect of the fermentation liquid or solid culture obtained in microbial manure, microbial pesticide or feed addictive.
Preferably, it is described fermentation obtain fermentation liquid, then add 0-99.99 parts by decomposed feces of livestock and poultry or contain
The industrial by-products of machine matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol,
0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into micro-
Bio-feritlizer, microbial pesticide or feed addictive；The solid culture, then add 0-99.99 parts by decomposed poultry
Poultry manure or industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea,
0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of sulphur
Sour potassium is prepared into microbial manure, microbial pesticide or feed addictive.
Further, the fermentation liquid that the fermentation obtains is prepared into living containing 10,000,000,000-5,000 hundred million/g by spray drying
The bacterium powder of bacterium, then add 0-99.99 parts by decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts it is yellow rotten
Sour potassium, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005
Part ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into microbial manure, microbial pesticide or feed
Additive；Cyclonic separation solid fermentation object obtains pure conidia powder, then add 0-99.99 parts by decomposed feces of livestock and poultry or contain
The industrial by-products of organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts it is sweet
Oil, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into
Microbial manure, microbial pesticide or feed addictive.
Above-mentioned all when preparing microbial manure, microbial pesticide or feed addictive, it is above-mentioned part can be added
Raw material can also add all raw materials above-mentioned, or not use raw material above-mentioned.
Technical solution of the present invention is described in detail combined with specific embodiments below.
Embodiment one:
The first step, by swine manure wastewater by being separated by solid-liquid separation, liquid phase handles 14 days into biochemical sewage processing pond, obtains 0.82
Part supernatant.
0.04 part of bean cake powder, 0.03 part of maize flour, 0.01 part is added in second step in the supernatant that the first step obtains
Glucose, 0.002 part of peptone, 0.003 part of yeast powder, 0.001 part of manganese sulfate, 0.002 part of potassium dihydrogen phosphate,
0.001 part of magnesium sulfate.It is sufficiently mixed, pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes.
Cultured bacillus amyloliquefaciens strain is inoculated into the culture medium of second step preparation by third step, and 37 DEG C, logical
Gas ratio is that 0.5,60 turns/min cultivates 72h, and it is 16,800,000,000/ml that viable count is detected after culture.
4th step adds 0.4 part of potassium fulvate, 0.5 part of potassium dihydrogen phosphate, 0.05 in the fermentation liquid that third step obtains
Part urea, 0.005 part of glycerol, 0.005 part of ammonium molybdate, 0.005 part of ferrous sulfate, are made liquid microbe bacterium after mixing
Agent.The utilization rate of fertilizer and disease resistance, resistance of crop can be effectively improved, moreover it is possible to be obviously improved the quality and mouthfeel of crop.
Embodiment two:
The first step will rinse sewage caused by farm by being separated by solid-liquid separation, place, obtain 0.84 portion of supernatant.
Second step, the supernatant that the first step is obtained are added to 0.8 part of wheat bran, 0.6 part of maize flour, 0.3 part of beans
Dregs of rice powder, 0.001 part of manganese sulfate, 0.003 part of potassium dihydrogen phosphate, 0.001 part of magnesium sulfate mixture in, 121 ± 2 DEG C of sterilizings
120 minutes.
Cultured Trichoderma strain is inoculated into the culture medium of second step preparation by third step, 28 DEG C, culture 96h, training
It is 5,900,000,000/g that spore count living is detected after supporting.The culture of the method preparation is organic matter decomposing inoculant, in straw-returning
It is added in straw in millesimal ratio, straw decomposing cycle time can be made 10-30 days, and the anti-of crop can be promoted
Characteristic of disease.
Embodiment three:
Feces of livestock and poultry is fermented 25 days by methane-generating pit, is separated by solid-liquid separation, obtains 0.64 portion of supernatant by the first step.
0.01 part of bean cake powder, 0.01 part of maize flour, 0.02 part is added in second step in the supernatant that the first step obtains
Glucose, 0.001 part of peptone, 0.001 part of yeast powder, 0.0005 part of manganese sulfate, 0.0005 part of potassium dihydrogen phosphate,
0.005 part of magnesium sulfate.It is sufficiently mixed, pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes.
Cultured Bacillus subtilis strain is inoculated into the culture medium of second step preparation by third step, 35 DEG C, ventilation
Than being 1.0,160 turns/min culture 48h, it is 13,600,000,000/ml that viable count is detected after culture.
4th step, the fermentation liquid that third step is obtained are prepared by spray drying containing 10,000,000,000-5,000 hundred million/g viable bacterias
Bacterium powder, is prepared into feed addictive, and 5/10000ths are added in egg feedstuff, can effectively improve the utilization rate of feed, and can mention
The disease resistance of high laying hen.
Example IV:
The first step, by livestock and poultry cultivation sewage by being separated by solid-liquid separation, liquid phase handles 5 days into biochemical sewage processing pond, obtains 0.84 part
Supernatant.
0.08 part of bean cake powder, 0.06 part of maize flour, 0.03 part is added in second step in the supernatant that the first step obtains
Glucose, 0.005 part of peptone, 0.005 part of yeast powder, 0.005 part of manganese sulfate, 0.005 part of potassium dihydrogen phosphate,
0.0005 part of magnesium sulfate.It is sufficiently mixed, pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes.
Cultured bacillus thuringiensis strain is inoculated into the culture medium of second step preparation by third step, and 32 DEG C, logical
Gas ratio is that 1.5,300 turns/min cultivates 20h, and it is 9,800,000,000/ml that viable count is detected after culture.
Third step is obtained fermentation liquid by spray drying, is prepared into the bacterium containing 10,000,000,000-5,000 hundred million/g viable bacterias by the 4th step
Powder, microbial source low toxic pesticide is obtained, for preventing and treating Orthoptera, coleoptera, Diptera, Hymenoptera, especially Lepidoptera
Various pests.
Embodiment five:
Livestock and poultry cultivation sewage is fermented 20 days by methane-generating pit, is separated by solid-liquid separation, obtains 0.86 portion of supernatant by the first step.
Second step, the supernatant that the first step is obtained are added to 0.3 part of wheat bran, 0.8 part of maize flour, 0.2 part of beans
Dregs of rice powder, 0.0005 part of manganese sulfate, 0.005 part of potassium dihydrogen phosphate, 0.0005 part of magnesium sulfate mixture in, 121 ± 2 DEG C go out
Bacterium 120 minutes.
Cultured bacillus thuringiensis strain is inoculated into the culture medium of second step preparation by third step, 32 DEG C, training
50h is supported, it is 3,500,000,000/g that viable count is detected after culture.
Third step is obtained the potassium dihydrogen phosphate that solid fermentation object crushes, then adds 0.05 part, is prepared into micro- by the 4th step
Biological source low toxic pesticide, for preventing and treating the various pests of Orthoptera, coleoptera, Diptera, Hymenoptera, especially Lepidoptera.
Embodiment six:
The first step, by feces of livestock and poultry by being separated by solid-liquid separation, liquid phase is handled 20 days, is obtained on 0.62 part into biochemical sewage processing pond
Clear liquid.
Second step, the supernatant that the first step is obtained are added to 1.2 parts of wheat bran, 0.2 part of maize flour, 0.6 part of beans
Dregs of rice powder, 0.005 part of manganese sulfate, 0.0005 part of potassium dihydrogen phosphate, 0.005 part of magnesium sulfate mixture in, 121 ± 2 DEG C go out
Bacterium 120 minutes.
Cultured Bacillus subtilis strain is inoculated into the culture medium of second step preparation by third step, 30 DEG C, culture
For 24 hours, it is 6,400,000,000/g that spore count living is detected after culture.
The solid fermentation object that third step obtains is added 1 part of potassium fulvate, 0.5 part of potassium dihydrogen phosphate, 55 by the 4th step again
Urea, 0.05 part of glycerol, 0.005 part of ammonium molybdate, 0.005 part of ferrous sulfate, 60 parts of monoammonium phosphate, 50 parts of sulfuric acid of part
Potassium is prepared into feed addictive, and 5/10000ths are added in egg feedstuff, can effectively improve the utilization rate of feed, and can improve
The disease resistance of laying hen.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (4)
1. a kind of utilize swine manure wastewater, the method for aquaculture wastewater fermented and cultured microorganism, which is characterized in that including walking as follows
It is rapid:
Step 1: the processing of swine manure wastewater, aquaculture wastewater
By livestock and poultry cultivation sewage by separation of solid and liquid, 0.75-0.95 portions of supernatants are taken, it is spare；
Or by feces of livestock and poultry by being separated by solid-liquid separation, placement takes 0.64-0.82 portions of supernatants, spare；
Or feces of livestock and poultry, aquaculture wastewater ferment 20-55 days in methane-generating pit, it is separated by solid-liquid separation, takes 0.66-0.86 portions of supernatants,
It is spare；
Or by feces of livestock and poultry, aquaculture wastewater by being separated by solid-liquid separation, liquid phase is handled 5-20 days, is taken into biochemical sewage processing pond
0.62-0.84 portions of supernatants, it is spare；
Step 2: the preparation of culture medium
The preparation of fluid nutrient medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, add 0.01-
0.08 part of bean cake powder, 0.01-0.06 parts of maize flour, 0.01-0.03 parts of glucose, 0.001-0.005 parts of peptone,
0.001-0.005 parts of yeast powder, 0.0005-0.005 parts of manganese sulfate, 0.0005-0.005 parts of potassium dihydrogen phosphate,
0.0005-0.005 parts of magnesium sulfate, are sufficiently mixed, and pH is transferred between 6.8-7.5, and 121 ± 2 DEG C sterilize 30 minutes；
The preparation of solid medium: the supernatant of first step preparation and clear water are mixed with the ratio of 1:0-10, are then added to
0.3-1.2 parts of wheat bran, 0.2-0.8 parts of maize flour, 0.2-0.6 parts of bean cake powder, 0.0005-0.005 parts of manganese sulfate,
In 0.0005-0.005 parts of potassium dihydrogen phosphate, 0.0005-0.005 parts of sulfuric acid magnesium compounds, 121 ± 2 DEG C sterilize 120 minutes；
Step 3: inoculated and cultured
By bacillus subtilis, bacillus thuringiensis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus megaterium, silicon
Hydrochlorate bacillus, saccharomycete, rice aspergillus, Trichoderma or Actinomycetes species are inoculated into liquid or solid culture medium；Liquid training
The condition of culture for supporting base is 32-37 DEG C, ventilation ratio 0.5-1.5, and revolving speed is that 60-300 turns/min, is cultivated 20-72 hours, fermentation
Obtain fermentation liquid；The condition of culture of solid medium is 28-32 DEG C, and periodic agitation is cultivated 24-96 hours, obtains solid culture
Object.
What 2. a kind of method using swine manure wastewater, aquaculture wastewater fermented and cultured microorganism described in claim 1 obtained
The effect of fermentation liquid or solid culture in microbial manure, microbial pesticide or feed addictive.
3. application according to claim 2, which is characterized in that the fermentation liquid that the fermentation obtains, then add 0-99.99 parts
By decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate,
0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of phosphoric acid one
Ammonium, 0-50 parts of potassium sulfate, is prepared into microbial manure, microbial pesticide or feed addictive；The solid culture, then add
Add 0-99.99 parts by decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts
Potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60
The monoammonium phosphate of part, 0-50 parts of potassium sulfate, are prepared into microbial manure, microbial pesticide or feed addictive.
4. application according to claim 2, which is characterized in that the fermentation liquid that the fermentation obtains, by spray drying, system
It is standby at containing the bacterium powder of 10,000,000,000-5,000 hundred million/g viable bacterias, then add 0-99.99 part by decomposed feces of livestock and poultry or contain organic matter
Industrial by-products, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate, 0-55 parts of urea, 0-0.05 parts of glycerol, 0-
0.005 part of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of monoammonium phosphate, 0-50 parts of potassium sulfate, are prepared into micro- life
Object fertilizer, microbial pesticide or feed addictive；Cyclonic separation solid fermentation object obtains pure conidia powder, then adds 0-99.99 parts
By decomposed feces of livestock and poultry or the industrial by-products containing organic matter, 0-1 parts of potassium fulvates, 0-0.5 parts of potassium dihydrogen phosphate,
0-55 parts of urea, 0-0.05 parts of glycerol, 0-0.005 parts of ammonium molybdate, 0-0.005 parts of ferrous sulfate, 0-60 parts of phosphoric acid one
Ammonium, 0-50 parts of potassium sulfate, is prepared into microbial manure, microbial pesticide or feed addictive.
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| CN111517866A (en) * | 2020-06-02 | 2020-08-11 | 贵州省冶金化工研究所 | Organic fertilizer for agate red cherries prepared from pig manure modified edible fungus waste sticks and preparation method thereof |
| CN112499774A (en) * | 2020-12-01 | 2021-03-16 | 九江礼涞生物科技有限公司 | Livestock and poultry breeding sewage treatment agent and application thereof |
| CN113480346A (en) * | 2021-08-18 | 2021-10-08 | 安徽坤健生物科技有限公司 | Biological treatment process for preparing organic biofertilizer from chicken manure |
| CN114516764A (en) * | 2021-08-30 | 2022-05-20 | 鹤壁市禾盛生物科技有限公司 | Method for preparing bacillus fermentation culture medium by using agricultural and sideline product waste |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111115840A (en) * | 2019-10-18 | 2020-05-08 | 河南省科学院生物研究所有限责任公司 | Method for harmless treatment and resource recycling of waste of live pig farm |
| CN111517866A (en) * | 2020-06-02 | 2020-08-11 | 贵州省冶金化工研究所 | Organic fertilizer for agate red cherries prepared from pig manure modified edible fungus waste sticks and preparation method thereof |
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| CN113480346A (en) * | 2021-08-18 | 2021-10-08 | 安徽坤健生物科技有限公司 | Biological treatment process for preparing organic biofertilizer from chicken manure |
| CN114516764A (en) * | 2021-08-30 | 2022-05-20 | 鹤壁市禾盛生物科技有限公司 | Method for preparing bacillus fermentation culture medium by using agricultural and sideline product waste |
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