CN1246284A - Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry - Google Patents
Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry Download PDFInfo
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Abstract
A process for preparing liquid or solid microbe insecticide features that the high-concentration waste organic liquid generated in fermentation industry (or the pretreated one) and the natralized Bt spawn are used to culture Bcillus thuringiensis in fermentor, and the fermented liquid is properly treated treated to obtain said microbe insecticide which has high insecticiding effect and no (or low) poison to higher living things. Its advantages include treating waste liquid, simple process, less equipment, low energy consumption, use of waste and low cost.
Description
The present invention relates to the fermentation industry waste water reuse, particularly a kind of method of utilizing high-concentration waste organic water in fermentation industry to produce microorganism insecticide.
The product of fermentation industry mainly obtains by the microbial liquid fermented extracted is refining, though the waste liquid that this process produces part belongs to high concentrated organic wastewater, they are the primary pollution source of fermentation industry.COD in the high concentrated organic wastewater (chemical oxygen demand) and BOD (biochemical oxygen demand (BOD)) all substantially exceed the wastewater discharge standard of national regulation.If prior art makes their reach national specified discharge standard through handling, invest very big and fees of maintenance are also high.On the other hand, the high concentrated organic wastewater in the fermentation industry usually contains abundant recycling organic substance, and how to utilize them cost-effectively also is the problem that people make great efforts to research and solve always.
Insecticidal microorganism can be killed insect, and seldom contaminated environment, and insect is difficult to it is developed immunity to drugs, and does not generally also injure the natural enemy of energy Pest Control group.Such microorganism has comprised bacterium, fungi, virus and protozoa etc.The 100 various insects pathogens that identified are adhered to Bacillaceae (Bacillaceae), micrococcaceae (Microccaceae), Lctobacteriaceae (Lactobacillacece) and pseudomonadaceae (Pseudomonadaceae) etc. separately.Wherein has only thuringiensis (Bacillus thuringiensis) in the Bacillaceae (Bacillaceae) by large-scale production and application.Its preparation extensively is used to control agricultural (as grain, vegetables, cotton, melon and fruit and cigarette dish etc.) insect, forestry pest, storage insect and environmental sanitation insect etc., through using on a large scale decades, prove that it is a kind of in the world very fruitful microorganism insecticide, output has accounted for more than 90% of microbial pesticide total amount, thereby occupies an important position in the microbial control damage by disease and insect.Along with the mankind to the further attention of ecotope and going deep into of research, its demand also can be increasing.
The thuringiensis microbial pesticide abbreviates the Bt biopesticide usually as, and it mainly is that method by large scale fermentation obtains.In the high concentrated organic wastewater of fermentation industry, the nutrition that generally all contains a lot of Bt propagation and produce the desinsection composition.The high concentrated organic wastewater treating capacity of fermentation industry such as monosodium glutamate and alcohol is very big, if can be with they raw materials as fermented and cultured Bt, can turn waste into wealth, promptly when solving contaminated wastewater, can obtain ecological benefits and the good microbial pesticide of economic benefit again in a large number.
The purpose of this invention is to provide and utilize high-concentration waste organic water in fermentation industry to carry out extensive Bt fermentation, so that handle the method for high concentrated organic wastewater and a large amount of Bt of acquisition quasi-microorganism insecticides cost-effectively as a kind of raw materials for production.
Another object of the present invention provides a kind of product that adopts said method to make.
The method of utilizing high concentrated organic wastewater to produce microorganism insecticide of the present invention comprises Wastewater Pretreatment, strain domestication, fermentation medium nutritional supplementation, fermentation and concentration process.
Wherein, the main purpose of described Wastewater Pretreatment be before fermentation, reduce in the waste water to Bt propagation and produce the composition that the desinsection composition has serious side effects, so that these side effects are obviously reduced.Give processing method and comprise that the metallic element concentration sum of working as in the waste water greater than 3500mg/l, adopts ion-exchange resin technique, will have the heavy metal composition of serious side effects to remove to production selectively.
Describedly give processing and can also comprise sulfate radical SO in the waste water
4 2-Concentration was greater than 0.5% o'clock, and the oxide that can add calcium makes their reactions generate CaSO
4Precipitation utilizes gravitational settling to separate then or centrifugal separation method is removed the partly precipitated thing.Containing SO
4 2-Waste water in add the oxide of 0.5% to 5% calcium, the oxide of described calcium is CaCO
3, CaO and Ca (OH)
2To generate CaSO
4Precipitation.Add the amount of the oxide of calcium and should guarantee that sulfate concentration free in the waste water is less than 0.5%.In this process, should give the waste water agitation as appropriate, guarantee reaction uniformity; And utilize gravitational settling separation or centrifugal separation method to remove solid-state CaSO
4, guarantee not contain in the zymotic fluid too much sediment.Add the oxide of calcium and the CaSO that reaction generates
4Precipitation removal process can be carried out in same production equipment.
It is described that to give processing method be not requisite or divide successively.Removing some metal ion as ion exchange resin technology also can at first carry out.The selection of ion exchange resin should be decided according to concrete waste water composition and employed bacterial classification character.But the SO in waste water
4 2-Content was greater than 0.5% o'clock, and fermentation is less stable as a result, and the oxide step that add calcium this moment is needs.The oxide of calcium is generally CaCO
3, CaO and Ca (OH)
2The amount of adding should guarantee that sulfate concentration free in the waste water is less than 0.5%.
The domestication of described Bt bacterium is in order to improve the adaptive capacity of Bt bacterial classification to some composition in the waste water, to obtain the zymotic fluid that high desinsection tires and to reduce production costs.Domestication can be inoculated back 30 ℃ and cultivate 72 hours on inclined-plane, flat board and shaking table, carry out repeatedly.Through tens generations or hundreds of generation domestication cultivate, can filter out the Bt bacterial strain stronger to the waste water adaptive capacity.The desinsection of the bacterial strain after waiting to tame tire near or when reaching the desinsection of original starting strain in normal medium and tiring, promptly can be used in the production.For accelerating this domestication process and strengthening the adaptive capacity of Bt bacterial classification, can also in medium, manually add some has serious side effects to Bt propagation and generation desinsection composition composition to some composition in the waste water.In general, all should repeat this domestication process to every class high concentrated organic wastewater and could obtain the result of fermenting preferably from original Bt bacterial classification.
Carry out the Bt strain domestication on inclined-plane and flat board, used culture medium prescription is generally:
Beef extract 0.3%, peptone 1.0%, NaCl 0.5%, agar 2.0%, all the other are original waste water or pretreated waste water.
When the sulfate concentration in the waste water is higher,, can in above-mentioned general culture medium prescription, add (NH for accelerating the domestication process
4)
2SO
41.0%-5.0%.
Carrying out the used culture medium prescription of Bt strain domestication on shaking table is generally:
Soybean cake powder 1.0%, dusty yeast 1.0%, starch 1.0%, all the other are original waste water or pretreated waste water.
When the sulfate concentration in the waste water is higher,, also can in above-mentioned shaking table culture medium prescription, add (the NH of 1.0%-5.0% for accelerating the domestication process
4)
2SO
4
After end is cultivated in each domestication, all should examine under a microscope and tame the result and pick out crystal and the bacterial strain of the further domestication of all normal or basic normal Bt bacterial strain conduct of gemma.If occurred thalli morphology in the domestication process all than normal but the parasporal crystal shape has the bacterial strain than big-difference, then should tame respectively.Domestication is cultivated these has the universal law than the bacterial strain of big-difference to be: after domestication on inclined-plane and the flat board ten times, again in twice of domestication on the shaking table and get domestication is cultivated on the last shaking table result and carry out the desinsection titration and relatively be worth further domestication to be cultivated with which bacterial strain of decision next round.So continuous domestication is cultivated and screening is tired approaching or reached the desinsection of original starting strain in normal medium up to the desinsection of the bacterial strain after the domestication tires.
Described fermentation medium nutritional supplementation is in order to obtain zymotic fluid that high desinsection tires to reduce production costs.The nutrition of adding is mainly carbon source, nitrogenous source and mineral matter.Carbon source nutrition mainly comes self-carbon water compound, comprises the liquid of corn starch, potato starch, soy noodle, corn flour, glucose and carbohydrate containing; Nitrogenous source nutrition comprises fish meal, dusty yeast, soybean cake powder, corn steep liquor and collagen mainly from the organic nitrogenous source; Described mineral matter comprises potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, potassium sulphate, manganese sulphate, zinc sulphate.The exact amount of each nutriment that adds in waste water should be decided on nutrition content in the waste water and the concentrated degree of waste water.The summation of total reducing sugar (calculating) content in the ferment culture fluid and total nitrogen content of starting originally with glucose surpass 3% and C/N between the 0.5-5 and mineral nutrition composition sum between 0.02%-1.5% the time, can obtain the zymotic fluid of high toxicity evaluation.Each nutriment that is promptly added should guarantee the summation of total reducing sugar (calculating) content in the fermentation culture and total nitrogen content with glucose surpass 3% and C/N between the 0.5-5 and other mineral nutrition composition sum between 0.02%-1.5%.
Described fermentation process can adopt efficient airlift reactor or stirring reactor as the wastewater fermentation jar.When adopting efficient airlift reactor, the Ventilation Rate of fermentation process is the gas flow rate under standard state is calculated) between the 0.01-0.15m/s, cultivation temperature is 26-37 ℃.When adopting stirring reactor, the ventilation of fermentation process is than between 0.03-3, and cultivation temperature is 26-37 ℃.After fermented and cultured finishes, available traditional heating evaporation, centrifugal separation method or membrane separation technique concentrated broth.It is fixed that the multiple whether zymotic fluid concentrates and concentrate mainly comes according to the kind of Bt biopesticide preparation.When preparation was pulvis, concentrated general times generally carried out atomized drying and packing again after 3-10 times.When preparation was liquor, concentrated general times generally at 1-3 doubly.Obtaining can long preservation and increase the preparation of insecticidal effect again, also should add stabilizing agent and synergist in the last handling process of zymotic fluid.
The method of utilizing high concentrated organic wastewater to produce microorganism insecticide of the present invention can also be included as and make full use of waste water, waste water is concentrated 1-10 utilize after doubly again, also can zymotic fluid be given to concentrate after fermentation.The method that concentrates has heating evaporation, centrifugation or membrane filtration isolation technics.
The methodological science that utilizes high concentrated organic wastewater to produce microorganism insecticide of the present invention is reasonable, utilized the high concentrated organic wastewater of fermentation industry completely effectively, turn waste into wealth, both solved the waste water pollution problem of fermentation industry, obtain again can prevention and elimination of disease and pests microorganism insecticide, ecological benefits, obvious social benefit.Employed thuringiensis should be through behind the acclimation and screening this waste water being had the bacterial strain of strong adaptive capacity in its employed waste water.
Further describe the present invention below in conjunction with embodiment.
Embodiment 1
Sulfate radical in the high concentrated organic wastewater that a lot of Gourmet Powder Factories produce, because glutami acid fermentation liquor will add the concentrated sulfuric acid when isoelectric point is separated glutamic acid, therefore sulfate concentration is up to more than 1%, the scope that surpasses conventional microorganism adaptation far away, this also is a difficult point handling gourmet powder waste water now with bioanalysis.Utilize this kind waste water to produce insecticide, at first constantly domestication Bt bacterium in these waste water filters out the bacterial classification that can adapt to this environment, simultaneously also will be to the processing of giving before the waste water to ferment.
Detailed process is:
Get Gourmet Powder Factory through isoelectric point separate fermentation waste liquor COD content 78000mg/l behind the glutamic acid, after ion exchange resin further separates glutamic acid from handing over waste liquor COD content 35000mg/l, again after each 1 part of mixing of the waste liquor after the flocculation sediment Partial Protein (COD content 15000mg/l), use ICP, promptly the essential element component content of ICP-AES analysis-e/or determining is as follows:
Unit: mg/l
| ??S | ??Na | ???K | ??P | ??Mg | ??Ca | ??Fe | ??B | ??Ni | ??Cr |
| ??8072 | ??1487 | ??749 | ??191 | ??100.2 | ??52.8 | ??30.1 | ??6.01 | ??2.432 | ??1.39 |
| ??Mn | ??Sr | ??Ti | ??Ba | ??Zn | ??al | ??Co | ??Cu | ??V | ??Pb |
| ??3.13 | ??0.40 | ??0.13 | ??0.14 | ??0.45 | ??0.60 | ??0.10 | ??0.15 | ??0.06 | ??0.15 |
Can find out that from above the content of element S is higher than other each element far away in the gourmet powder waste water, presses SO
4 2-Calculate content about 1.8%.If this waste water is concentrated to 10 times, the SO in the waste water
4 2-Concentration increases considerably again, so the oxide that adds calcium this moment is to form CaSO
4The giving treatment step and can not economize of precipitation.Gourmet powder waste water does not concentrate in this example, adds technical grade CaCO
3Total amount be 2%, CaCO is added on the limit
3Stir on the limit.Reaction finishes the back and removes 50% consolidate and contain thing with natural subsidence, uses it for anything else in addition after the consolidating of removal contains the thing washing.Separated CaSO
4The waste water of post precipitation contains nitrogen 0.65% after measured, and therefore reducing sugar 0.51% needs the nutrition of interpolation as follows:
Corn starch 0.8% glucose 0.2% soybean cake powder 0.6%
Dusty yeast 0.4% corn steep liquor 0.3% MnSO
40.01%
MgSO
40.02％???ZnSO
40.02％
Carried out the fermented and cultured at stirred-tank fermenter Bt respectively with the waste water after the above-mentioned nutrition of interpolation, the employed Bt bacterial classification that ferments is the Bt bacterial classification after taming and screen through 25 generations in gourmet powder waste water.The domestication incubation is carried out on the inclined-plane, inoculates back 30 ℃ and cultivates 72 hours, and used culture medium prescription is: beef extract 0.3%, and peptone 1.0%, NaCl0.5%, agar 2.0%, all the other are pretreated waste water.For accelerating the domestication process, in above-mentioned culture medium prescription, add (NH
4)
2SO
43.0%.
Fermentation condition at stirred-tank fermenter is as follows:
1, bacterial classification: the Bt bacterial strain H in the fermentation waste liquor after isoelectric point is separated glutamic acid after domestication and the screening
3abType, inclined-plane inoculate back 30 ℃ cultivate 72 hours stand-by.
2, fermentation tank: the full automatic control stirring-type of MSJ-U3-30L that Japanese ball water chestnut company produces is sent out and is answered device.
3, controlled condition: pH transfers to the sterilization of 8.5,121 ℃ of steam heated after 25 minutes with NaOH before the sterilization, is cooled to 32 ℃, and sterilization after fermentation liquid constant volume is at 18L, 32 ℃ ± 0.2 ℃ of temperature control, ventilation 1.2m
3/ hr, tank pressure 0.05MPa, speed of agitator 250 is to 400r.p.m.
Ferment and take a sample after 45 hours, carry out insecticidal effect by national standard and measure.The result shows that the toxicity evaluation of zymotic fluid between the 2100IU/mg, approaches the fermentation result in the normal concentration medium at 1500IU/mg.
Embodiment 2
Gourmet Powder Factory's high concentrated organic wastewater sampling is with example 1.But for making full use of waste water, this example is for concentrating waste water.Earlier make waste water concentrate 2 times through the tunica fibrosa ultrafiltration system, other give handle and the strain domestication process with example 1, and ferment and in airlift fermentor, carry out.Fermenting took a sample after 48 hours carries out insecticidal effect mensuration by national standard, and the zymotic fluid toxicity evaluation is all more than 2000IU/mg.This illustrates the high concentrated organic wastewater that adopts after concentrating 2 times as the Bt fermentation raw material, not only feasible but also better effects if.Fermentation condition at airlift fermentor is as follows:
1, bacterial classification: with embodiment 1.
2, fermentation tank: the efficient airlift reactor of Ф 0.2m * 1.6m (structure is seen Chinese invention patent CN1186853a).
3, controlled condition: pH transfers to 8.5,121 ℃ of steam heated sterilizations after 25 minutes with NaOH before the sterilization, is cooled to
32 ℃, sterilization after fermentation liquid constant volume is at 36L, 30 ℃ ± 1 ℃ of temperature control, rate of venting 0.005m/s-0.06m/s, tank pressure 0.15MPa.
Embodiment 3
This example is produced the Bt microbial pesticide for alcohol waste water.Get potato do for the Alcohol Plant of primary raw material with centrifugal separation method the part in the alcohol residue is contained waste water after thing removes admittedly, as follows with the essential element composition of ICP plasma atomic emission spectrometer mensuration:
Unit: mg/l
| ??S | ??Na | ??K | ??P | ??Mg | ??Ca | ??Fe | ??B | ??Ni | ??Cr |
| ??50.9 | ??35.9 | ??721 | ??135 | ??112 | ??143 | ??103 | ??0.29 ??7 | ??0.34 ??3 | ??0.1 ??9 |
| ??Mn | ??Sr | ??Ti | ??Ba | ??Zn | ??al | ??Co | ??Cu | ??V | ??Pb |
| ??3.21 | ??0.56 ??3 | ??10.8 | ??1.31 | ??1.23 | ??87.6 | ??0.11 ??2 | ??1.12 | ??0.28 ??9 | ??0.4 ??5 |
Can find out obviously that from above the content of element S need not to implement to add the oxide of calcium to form CaSO in the gourmet powder waste water in the alcohol waste water
4Precipitation give processing.In this example this waste water is not had the concentrated processing of yet not taking to give, directly extra-nutrition promptly ferments, and the nutrition of interpolation is as follows:
Corn starch 0.8%, soybean cake powder 2.5% dusty yeast 0.4%
Corn steep liquor 1.2% P
2HPO
40.1% MnSO
40.02%
MgSO
40.02％???????ZnSO
40.02％????CaCO
30.3％
For accelerating the domestication process, the domestication of Bt bacterial classification is to carry out in the alcohol waste water that concentrated, the domestication incubation is carried out on the inclined-plane, inoculating back 30 ℃ cultivated 72 hours, used culture medium prescription is: beef extract 0.3%, and peptone 1.0%, NaCl 0.5%, agar 2.0%, all the other are pretreated waste water.Fermentation is carried out in efficient airlift fermentor, and fermentation condition is as follows:
1, bacterial classification: for taming the Bt bacterial strain H in 15 generations in the alcohol waste water after concentrating 2 times
3abType, inclined-plane inoculate back 30 ℃ cultivate 72 hours stand-by.
2, the airlift fermentor of fermentation tank Ф 0.2m * 1.6m (structure is with example 1).
3, controlled condition (with the airlift fermentor in the example 1).
Fermenting took a sample after 38 hours carries out insecticidal effect mensuration by national standard, and the zymotic fluid toxicity evaluation is between 1200IU/mg to 1600IU/mg.Zymotic fluid concentrates 2.5 times through the tunica fibrosa ultrafiltration system again, and toxicity evaluation surpasses 4000IU/mg.
Claims (10)
1, a kind of method of utilizing the high concentrated organic wastewater production microorganism insecticide of fermentation industry production generation is characterized in that comprising Wastewater Pretreatment, strain domestication, fermentation medium nutritional supplementation, fermentation and concentration process.
2, method according to claim 1 is characterized in that giving processing method and comprises that metallic element concentration sum in waste water greater than 3500mg/l, adopts ion-exchange resin technique, makes metallic element concentration sum in the waste water less than 3500mg/l.
3, method according to claim 1 is characterized in that describedly giving processing and also comprising sulfate radical SO in the waste water
4 2-Concentration was greater than 0.5% o'clock, and the oxide of the calcium of interpolation 0.5% to 5% makes their reactions generate CaSO
4Precipitation utilizes gravitational settling to separate then or centrifugal separation method is removed the partly precipitated thing, and the oxide of described calcium is CaCO
3, CaO and Ca (OH) z, addition should guarantee that sulfate concentration free in the waste water is less than 0.5%.
4, according to claims 1 described method, the domestication process that it is characterized in that described Bt bacterium is that each inoculation was cultivated 72 hours for back 30 ℃ on inclined-plane, flat board and shaking table, carries out repeatedly;
Carry out the Bt strain domestication on inclined-plane and flat board, used culture medium prescription is:
Beef extract 0.3%, peptone 1.0%, NaCl 0.5%, agar 2.0%, all the other are original waste water or pretreated waste water;
When the sulfate concentration in the waste water is higher, in above-mentioned culture medium prescription, add (NH
4)
2SO
41.0%-5.0%;
Carrying out the used culture medium prescription of Bt strain domestication on shaking table is:
Soybean cake powder 1.0%, dusty yeast 1.0%, starch 1.0%, all the other are original waste water or pretreated waste water;
When the sulfate concentration in the waste water is higher, in above-mentioned shaking table culture medium prescription, add (the NH of 1.0%-5.0%
4)
2SO
4
5, according to claims 1 described production method, what it is characterized in that described fermentation medium nutritional supplementation adds is carbon source, nitrogenous source and mineral matter; Carbon source nutrition comes self-carbon water compound, comprises the liquid of corn starch, potato starch, soy noodle, corn flour, glucose and carbohydrate containing; Nitrogenous source nutrition comprises fish meal, dusty yeast, soybean cake powder, corn steep liquor and collagen from the organic nitrogenous source; Described mineral matter comprises potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, potassium sulphate, manganese sulphate, zinc sulphate.
6, according to claims 1 described production method, the summation that it is characterized in that in the fermentation culture total sugar content that calculates with glucose and total nitrogen content surpass 3% and C/N between the 0.5-5 and mineral nutrition composition sum between 0.02%-1.5%.
7,, it is characterized in that described fermentation process adopts efficient airlift reactor or stirring reactor as the wastewater fermentation jar according to claims 1 described production method.
8, according to claims 1 described production method, it is characterized in that when adopting efficient airlift reactor the Ventilation Rate of fermentation process is that the gas flow rate under standard state is calculated is between the 0.01-0.15m/s, cultivation temperature is 26-37 ℃; When adopting stirring reactor, the ventilation of fermentation process is than between 0.03-3, and cultivation temperature is 26-37 ℃.
According to claims 1 described production method, it is characterized in that when preparation is pulvis that 9, concentrated general times carries out atomized drying and packing again after 3-10 times; When preparation was liquor, concentrated general times at 1-3 doubly.
10, according to claims 1 described production method, it is characterized in that also comprising that waste water is concentrated 1-10 carries out Wastewater Pretreatment, strain domestication, fermentation medium nutritional supplementation, fermentation and concentration process after doubly again, or after fermentation, zymotic fluid is given to concentrate, the method that concentrates has heating evaporation, centrifugation or membrane filtration isolation technics.
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|---|---|---|---|
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|---|---|---|---|
| CN 99119084 CN1120664C (en) | 1999-09-14 | 1999-09-14 | Process for preparing microbe insecticide with high-concentration waste organic water in fermentation industry |
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| CN1120664C CN1120664C (en) | 2003-09-10 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100367859C (en) * | 2006-01-17 | 2008-02-13 | 北京大学 | Method for preparing bacillus thuringiensis microbiological pesticide by tumeric hydrolysis waste liquor |
| CN102308851A (en) * | 2010-06-29 | 2012-01-11 | 中国科学院生态环境研究中心 | Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater |
| CN102796683A (en) * | 2012-08-07 | 2012-11-28 | 福建农林大学 | Glucoside fermentation medium for bacillus thuringiensis |
| CN107090420A (en) * | 2017-06-07 | 2017-08-25 | 青岛农业大学 | A kind of fermentation culture method of bacillus thuringiensis |
| CN109234198A (en) * | 2018-10-12 | 2019-01-18 | 柴彦亮 | A method of utilizing swine manure wastewater, aquaculture wastewater fermented and cultured microorganism |
| CN109652342A (en) * | 2019-02-01 | 2019-04-19 | 福建农林大学 | A method of Bt liquid fermentation medium is prepared by raw material of vegetable castoff |
-
1999
- 1999-09-14 CN CN 99119084 patent/CN1120664C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100367859C (en) * | 2006-01-17 | 2008-02-13 | 北京大学 | Method for preparing bacillus thuringiensis microbiological pesticide by tumeric hydrolysis waste liquor |
| CN102308851A (en) * | 2010-06-29 | 2012-01-11 | 中国科学院生态环境研究中心 | Bacillus thuringiensis insecticide produced by using sweet potato starch wastewater |
| CN102796683A (en) * | 2012-08-07 | 2012-11-28 | 福建农林大学 | Glucoside fermentation medium for bacillus thuringiensis |
| CN107090420A (en) * | 2017-06-07 | 2017-08-25 | 青岛农业大学 | A kind of fermentation culture method of bacillus thuringiensis |
| CN107090420B (en) * | 2017-06-07 | 2021-01-26 | 青岛农业大学 | Fermentation culture method of bacillus thuringiensis |
| CN109234198A (en) * | 2018-10-12 | 2019-01-18 | 柴彦亮 | A method of utilizing swine manure wastewater, aquaculture wastewater fermented and cultured microorganism |
| CN109652342A (en) * | 2019-02-01 | 2019-04-19 | 福建农林大学 | A method of Bt liquid fermentation medium is prepared by raw material of vegetable castoff |
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| Publication number | Publication date |
|---|---|
| CN1120664C (en) | 2003-09-10 |
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