CN109221135A - A kind of drug and preparation and application method for accelerating the healing of oil tea autumn branches - Google Patents
A kind of drug and preparation and application method for accelerating the healing of oil tea autumn branches Download PDFInfo
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- CN109221135A CN109221135A CN201811157112.2A CN201811157112A CN109221135A CN 109221135 A CN109221135 A CN 109221135A CN 201811157112 A CN201811157112 A CN 201811157112A CN 109221135 A CN109221135 A CN 109221135A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Agronomy & Crop Science (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to graft technology field, the drug and preparation and application method of a kind of quickening oil tea autumn branches healing are disclosed, by 25mg/L Nafusaku, 25mg/L butyric acid potassium and 0.05mg/L 2,4-D sodium composition；It is that analysis is pure.In the clear water of 1L, 25mg Nafusaku, 25mg butyric acid potassium and 0.05mg 2,4-D sodium are sequentially added；After addition, stirring and dissolving；It places and refrigerates 4-10 DEG C under cryogenic.After having cut stock and scion, with stock notch speed libation at an ancient wedding ceremony formula solution, fast transfer stock to scion notch brings formula solution sufficiently to fill scion notch, is repeated twice by stock；Whip graft is amplexiformed, and the forming layer of stock notch is at least aligned with scion notch forming layer on one side, is fixed with plant graft clip, grafting can be completed.25 days or so after grafting, graft union healing, than being improved 30 days or so without formula processing；Survival rate is 95% or more, improves 8% or more compared with without formula processing.
Description
Technical field
The invention belongs to graft technology field more particularly to it is a kind of accelerate oil tea autumn branches healing drug and prepare and
Application method.
Background technique
Currently, the prior art commonly used in the trade is such that oil tea is cross-pollinatd plant, the economy of sexual propagation offspring
Trait segregation is obvious, therefore produces and Nurse seed grafting or cuttage progress oil tea seedling breeding and popularization is mainly taken to answer
With.Oil tea cutting propagation has 50 years of researches and applicating history in China, but Camellia oleifera Cutting Seedling Raising does not solve morning at present
Phase richness root technology, root system is undeveloped, and the surrival rate of afforestation is low.Therefore, for a long time, in the effect of Camellia oleifera Cutting Seedling Raising and its afforestation
With some disputes are always existed in meaning, in production application it is still less.Currently, oil-tea sprout anvil graft technology is mainly deposited
The following aspects the problem of: the grafting of oil-tea sprout anvil is generally concentrated at 2-4 month, seasonal strong, and the anniversary of scion utilizes
Rate is not high, seasonal scarce work also easily occurs；Oil-tea sprout stock grafting seedling graft union is from base portion only 1-1.5cm or so, when plantation
Soil covering interface is easily returned, the insufficient phenomenon of healing often occurs in interface in addition, this easily feels stem rot or root-rot after seedling is planted
It is sick and dead.Graft union can be improved in autumn branches technology, and can not solved oil-tea sprout preferably by grafting seasonal effect
Anvil graft technology is subject to seasonal restrictions, the low problem of graft union.But oil tea autumn branches wound healing is slow, generally requires 60 days left sides
It is right could notch engagement, survival rate not exclusively or the risk of grafting failure and transfers 80% or so, this adds increased graft union
What is connect manages and protects cost.
In conclusion problem of the existing technology is: seasonal strong, scion the anniversary utilization rate of oil-tea sprout anvil grafting
Low, graft union is low easily to feel stem rot or root rot and death；Oil tea autumn branches can solve oil-tea sprout anvil graft technology shortcoming,
But its interface callus is formed slow or is difficult to form callus so that healing is slow, the graft union process of plant by environment and
The growth characteristics of itself influence.The wherein biological nature of itself, including callus form slow or are difficult to form callus, transfer
Forming layer is thin in termination process, it is difficult to alignment etc..Oil tea autumn branches are exactly because callus forms slow or is difficult to form callus
Tissue, so that healing is slow to need ability notch engagement in 60 days or so, it is difficult to which forming callus cannot then survive；Callus shape
At slow, the time is long, increases interface by the risk that germ infects and increases temperature and humidity and influenced the wind that callus is formed
Danger, these are all the low reasons of survival rate, and survival rate is 80% or so.Interface is difficult to form callus or healing time
It is long, it increases graft union not exclusively or cost is managed and protected in the risk of grafting failure and grafting.
Solve the difficulty and meaning of above-mentioned technical problem:
The graft union process of plant is influenced by environment and the growth characteristics of itself.The wherein biological nature of itself, including
Callus forms slow or is difficult to form callus, and forming layer is thin in grafting procedures, it is difficult to alignment etc..Oil tea autumn branches are just
It is because callus forms slow or is difficult to form callus, so that healing is slow or grafting is unsuccessful.Therefore it must promote oil
Tea plantlets grafting interface quickly forms callus.The method for handling graft union by growth hormone, is to speed up callus shape
At approach.But different plants, the response that callus is formed to growth hormone are different；Different growth hormone and combination,
Different amounts etc., the effect generated to plant callus are also not quite similar.
The present invention is the best hormone prescription for promoting the healing of oil tea autumn branches mouth obtained on the basis of abundant experiment,
And its simple application method.By the processing of the formula, the time of autumn branches healing can be accelerated, to improve oil tea seedling
The success rate of grafting, reduce grafting manages and protects cost.
Summary of the invention
In view of the problems of the existing technology, accelerate the drug and match that oil tea autumn branches heal the present invention provides a kind of
System and application method.
The invention is realized in this way a kind of drug for accelerating the healing of oil tea autumn branches, the quickening oil tea seedling are transferred
The drug for connecing healing is made of 25mg/L Nafusaku, 25mg/L butyric acid potassium and 0.05mg/L 2,4-D sodium.
Another object of the present invention is to provide a kind of preparation method of drug for accelerating the healing of oil tea autumn branches,
It is described accelerate oil tea autumn branches healing drug preparation method the following steps are included:
Step 1 sequentially adds 25mg Nafusaku, 25mg butyric acid potassium and 0.05mg 2,4-D in the clear water of 1L
Sodium；
Step 2, after addition, stirring and dissolving；It places and refrigerates 4-10 DEG C under cryogenic.
Another object of the present invention is to provide a kind of application method of drug for accelerating the healing of oil tea autumn branches,
The application method of the drug for accelerating the healing of oil tea autumn branches the following steps are included: grafted using whip graft or the mode of amplexiforming,
After having cut stock and scion, with stock notch speed libation at an ancient wedding ceremony formula solution, stock is shifted to scion notch, formula is brought by stock
Solution sufficiently fills scion notch；Stock notch speed libation at an ancient wedding ceremony formula solution, repetitive displacement is twice；Formula solution fills scion and cuts
After mouthful, engage stock with scion, the forming layer of stock notch is at least aligned with scion notch forming layer on one side, with plant graft
Clip is fixed, and grafting is completed；The seedling for completing grafting is put on seedbed, by plastic film Small plastic shed moisturizing, southern 5-8 month seedling
The degree of shading of bed is maintained at 85% or more, other months 50-70%；It is primary to lift film watering southern 5-8 month every 7 days, other
It does not need in month to lift film watering.
Another object of the present invention is to provide a kind of stocks of drug using the quickening oil tea autumn branches healing
The oil tea autumn branches for being 0.2-0.4cm with scion size.
In conclusion advantages of the present invention and good effect are as follows: graft union can be improved in autumn branches technology, and is not transferred
Seasonal effect is connect, oil-tea sprout anvil graft technology can be preferably solved and be subject to seasonal restrictions, the low easily susceptible problem of graft union.This
Invention is used by the proportion of a variety of growth hormone, can achieve the effect that wound healing at 25 days or so, at without formula
Reason improves 30 days or so, significantly shortens the time of grafting survival, reduces the risk of grafting management cost and grafting failure；
Success rate reaches 95% or more, improves 8%-10% compared with without formula processing.In addition, by 25mg/L Nafusaku, 25mg/L
Butyric acid potassium and 0.05mg/L 2,4-D sodium composition are soluble in water, and the method for handling graft union is simple to operation.
Therefore, the present invention produces and uses convenient, is easy to receive.
Detailed description of the invention
Fig. 1 is the preparation method flow chart of the drug of quickening oil tea autumn branches healing provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
It is slow for oil tea autumn branches wound healing, need 60 days or so could notch engagement, survival rate 80% or so,
It increases graft union not exclusively or grafts the risk to fail and graft the problem of managing and protecting cost.The present invention is in order to accelerate seedling
The time of graft union is used by the proportion of a variety of growth hormone, can achieve the effect that wound healing at 25 days or so,
The time of grafting survival is significantly improved, success rate reaches 95% or more.
Application principle of the invention is explained in detail with reference to the accompanying drawing.(this section will be rewritten)
The drug provided in an embodiment of the present invention for accelerating the healing of oil tea autumn branches is by 25mg/L Nafusaku, 25mg/L
Butyric acid potassium and 0.05mg/L 2,4-D sodium composition；It is that analysis is pure.
As shown in Figure 1, the preparation method of the drug provided in an embodiment of the present invention for accelerating the healing of oil tea autumn branches includes
Following steps:
S101: in the clear water of 1L, 25mg Nafusaku, 25mg butyric acid potassium and 0.05 mg 2,4-D are sequentially added
Sodium；
S102: after addition, stirring and dissolving；It places and refrigerates 4-10 DEG C under cryogenic.
Be suitable for stock and the scion size of the drug provided in an embodiment of the present invention for accelerating the healing of oil tea autumn branches be
The oil tea autumn branches of 0.2-0.4cm.Grafting method is whip graft or amplexiforms.After having cut stock and scion, with stock notch speed
Libation at an ancient wedding ceremony formula solution, fast transfer stock to scion notch bring formula solution sufficiently to fill scion notch by stock.General anvil
The wooden notch speed libation at an ancient wedding ceremony formula solution, repetitive displacement is twice.After formula solution sufficiently fills scion notch, connect stock with scion
It closes, the forming layer of stock notch is at least aligned with scion notch forming layer on one side, is fixed with plant graft clip, be can be completed and transfer
It connects.The seedling for completing grafting is put on seedbed, by plastic film Small plastic shed moisturizing, southern 5-8 month the degree of shading in seedbed keep
85% or more, other months 50-70%.Lifted film watering southern 5-8 month every 7 days once, other months do not need to lift film
Watering.
Application principle of the invention is further described below with reference to specific implementation example of the invention.
The drug of quickening oil tea autumn branches healing provided in an embodiment of the present invention, preparation method and use method include following step
It is rapid:
1 nursery requires
The Kaiyang plot for being not easy ponding should be selected as nursery.It is black that nursery need to be equipped with 85%-90% sunshade degree for top
The simple sunshade of high flat-top or arch of color sunshade net.Ridging is seedbed, the high 10-15cm in seedbed ridge, wide 120- under canopy
150cm, ridge face cover mulch.
2. stock is cultivated
Stock container nursery is carried out using the forest seedling growth disk in the cave of 52 × 28cm, 32 or 50.Seedling medium is Light media.Anvil
Wood should select the local camellia oleifera cultivar good with scion affinity.The seed acquired on stock variety plant, through 800 times of carbendazim medicines
After ten minutes, being seeded in will be in the forest seedling growth disk of good matrix for agent soaking disinfection.It is long to 10cm or more trunk diameter to seedling
It can be used for grafting when 2mm-4mm.
The preparation of 3 formulas
3.1 experimental materials and reagent
Nafusaku, butyric acid potassium, 2,4-D sodium are that analysis is pure.
The preparation of 3.2 formulas
Formula is that the mixing containing 20mg/L Nafusaku, 20mg/L butyric acid potassium and 0.05mg/L 2,4-D sodium is molten
Liquid.Prepare example: in the clear water (pH value neutral) of 1L, sequentially add 20mg Nafusaku, 20mg butyric acid potassium and
When latter drug is added, dissolution former drug should be sufficiently stirred in 0.05mg 2,4-D sodium.Prepared formula can be placed in
(4-10 DEG C) is refrigerated under cryogenic conditions, but must be used in 7 days.
4. grafting and formula treatment process
The present invention should be under weather conditions of the temperature higher than 10 DEG C.Grafting method is whip graft.
4.1 fringe bars acquire and cut fringe
Fringe bar should be the local oil tea breeding of local popularization, and have preferable affinity with stock.Selection 1 year or 2 years
Raw axillary bud is full and diameter 2mm-4mm branch.Branch is cut into long 2-4cm scion, one leaf of scion one (armpit) bud, on
End is mitered into the inclined-plane of a length of 1.5cm or so in lower end axillary bud opposite side away from 2mm-3mm at axillary bud；Then, about oblique
Face middle, penetraction depth 1.0cm parallel with fringe bar or so, opens the notch of long 1.0cm or so.Fringe bar axillary bud is answered full.
Shearing fringe bar should be immediately placed in clear water or cover moisturizing with wet cloth, and be grafted within 1.0 or so hours.
4.2 cut anvil
Seedling should reach stock requirement.Seedling top trunk is wiped out, only retains 10-20cm base portion as stock.On trunk
Blade can retain if do not influenced to cut anvil operation.It splays in stock upper end, is cut into the inclined-plane of a length of 1.5cm or so；So
Afterwards, the notch of long 1.0cm or so about is opened in inclined-plane middle, penetraction depth 1.0cm parallel with stock or so.
4.3 engagements are handled with formula
After having cut stock and scion, with stock notch speed libation at an ancient wedding ceremony formula solution, fast transfer stock passes through to scion notch
Stock brings formula solution sufficiently to fill scion notch.General stock notch speed libation at an ancient wedding ceremony formula solution, repetitive displacement is twice.Match
After square solution sufficiently fills scion notch, engage stock with scion, the forming layer of stock notch at least one side and scion notch
Forming layer alignment, is fixed with plant graft clip, grafting can be completed.
4.4 managing after grafting
By equipped with the seedlings nursing plate that grafting seedling is completed, fitly it is placed on the seedbed got ready.Grafting seedling is sprayed
+ 800 times of liquid agricultural streptomycin solution of 800 times of liquid carbendazim prevent graft union infection from rotting.High 50cm or so is erected on seedbed
Small plastic shed, cover transparent plastic foil, seal, to keep high humility in arched shed.Southern 5-8 month seedbed degree of shading keep
85% or more, other months 50-70%.Lifted film watering southern 5-8 month every 7 days once, other months do not need to lift film
Watering.
It grafts 1-2 months or so, when the expansion of grafting axillary bud, can check the interface healing state of grafting.Such as 90%
The above healing completely, can lift film.
It should not be docked before healing and transfer seedling application liquid manure.After healing, sunshade net is gradually removed, can suitably spray or is poured with nitrogen
Liquid manure based on fertilizer, to promote axillary bud sprouting.When height of seedling 30cm or more can be out of the garden afforestation.
Application effect of the invention is explained in detail below with reference to experiment.
One, experimental method and material
Experimental period: three batches of graftings are carried out in September in 2017 3 days, on January 5th, 2018 and on May 1st, 2018 respectively.
Test place: Haikou City, Hainan Province Qiongshan District Hainan Shanxi Academy of Agricultural Sciences nursery.
Experimental material: fringe bar is the oil tea breeding " excellent No. 1 of fine jade section " Jing Guo provincial identification, and stock is same strain Hainan
The seed seedling-raising of tea oil tree.
Experimental design: being carried out in a manner of whip graft, to be control without formula processing.Each grafting method sets three weights
It is multiple.50 plants of oil teas of each repetition.
Record and statistics: observation in every seven days is primary, and record starts the time of healing and the time of all healed.Finally count
Strain number is survived, graft survival rate is calculated.
All steps of experiment press progress of the present invention.
Two, experimental result
(1) influence of the formula processing to healing time
As it can be seen from table 1 treated that healing time is dramatically speeded up for formula, compared with clear water processing, healing time shifts to an earlier date 26-
29d.After formula handles notch, the time of healing is completed between 20-26d.And without formula processing, complete the time of healing
Between 48-55d.
In addition, grafting season also will affect the graft union time；There is certain relationship with temperature of shed, 5-7 month is sea
In the high temperature month in mouth city, temperature is between 25-38 DEG C, and maximum temperature is between 38-47 DEG C in film；And the month Haikou 1-2 temperature
Annual minimum, temperature is between 10-18 DEG C, and maximum temperature is between 16-27 DEG C in film.Show that a certain range of high temperature is conducive to
Accelerate the healing of graft union in the present invention.But whether in which grafting, treated that healing time is intended to than not for formula in season
It handles and shifts to an earlier date through formula.
1 Grafting experiments result of table
(2) influence of the formula processing to grafting success rate
As it can be seen from table 1 in the present invention, be formulated the survival rate of processing than clear water processing be higher by 8%-10% it
Between, wherein the survival rate of processing is formulated between 96%-100%, and the survival rate of clear water processing is between 88%-96%.This
Outside, the survival rate of high temperature in May, grafting is minimum；And the temperature at Haikou in January is 10-18 DEG C, temperature of shed at this time is more
Properly, the survival rate of grafting is also higher, can reach 100%.As it can be seen that high temperature season grafting can reduce graft survival rate.Study carefully its original
Cause, it may be possible to which high temperature can make notch withered or pathogen breeding, increase the probability of invasion notch.
In conclusion formula processing can shorten graft union time 26-29d, graft survival rate 8%-10% is improved.Suitably
High temperature can be accelerated to heal, but the also corresponding survival rate for reducing grafting.The use of this patent formula can shorten healing time,
Reduce the probability of infection of incisional wound necrosis.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (4)
1. a kind of drug for accelerating the healing of oil tea autumn branches, which is characterized in that the medicine for accelerating the healing of oil tea autumn branches
Object is made of 25mg/L Nafusaku, 25mg/L butyric acid potassium and 0.05mg/L 2,4-D sodium.
2. a kind of preparation method for the drug for accelerating the healing of oil tea autumn branches as described in claim 1, which is characterized in that described
Accelerate oil tea autumn branches healing drug preparation method the following steps are included:
Step 1 sequentially adds 25mg Nafusaku, 25mg butyric acid potassium and 0.05mg 2,4-D sodium in the clear water of 1L；
Step 2, after addition, stirring and dissolving；It places and refrigerates 4-10 DEG C under cryogenic.
3. a kind of application method for the drug for accelerating the healing of oil tea autumn branches as described in claim 1, which is characterized in that described
The application method for accelerating the drug of oil tea autumn branches healing carries out the following steps are included: grafting by whip graft or in a manner of amplexiforming,
After having cut stock and scion, with stock notch speed libation at an ancient wedding ceremony formula solution, stock is shifted to scion notch, formula is brought by stock
Solution sufficiently fills scion notch；Stock notch speed libation at an ancient wedding ceremony formula solution, repetitive displacement is twice；Formula solution fills scion and cuts
After mouthful, engage stock with scion, the forming layer of stock notch is at least aligned with scion notch forming layer on one side, with plant graft
Clip is fixed, and grafting is completed；The seedling for completing grafting is put on seedbed, and plastic film Small plastic shed moisturizing, the southern china 5-8 month are passed through
The degree of shading in part seedbed is maintained at 85% or more, other months 50-70%；Lifted film watering one southern china 5-8 month every 7 days
It is secondary, it does not need in other months to lift film watering.
4. it is a kind of using described in claim 1 quickening oil tea autumn branches healing drug stock and scion size be 0.2-
The oil tea autumn branches of 0.4cm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113892355A (en) * | 2021-09-17 | 2022-01-07 | 云南省农业科学院热带亚热带经济作物研究所 | Auxiliary agent for coffee grafting and preparation method thereof |
| CN117941695A (en) * | 2024-03-22 | 2024-04-30 | 海南省农业科学院三亚研究院(海南省实验动物研究中心) | Method for improving seed yield of fresh camellia oleifera fruits, medicament and application of medicament |
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