CN109220548A - Residue is extracted as Medium for Ganoderma lucidum of major ingredient and preparation method thereof using agilawood essential oil - Google Patents
Residue is extracted as Medium for Ganoderma lucidum of major ingredient and preparation method thereof using agilawood essential oil Download PDFInfo
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- CN109220548A CN109220548A CN201811208424.1A CN201811208424A CN109220548A CN 109220548 A CN109220548 A CN 109220548A CN 201811208424 A CN201811208424 A CN 201811208424A CN 109220548 A CN109220548 A CN 109220548A
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- essential oil
- ganoderma lucidum
- residue
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- Environmental Sciences (AREA)
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- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
Residue is extracted as the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil the invention discloses a kind of, include following components according to mass percent: agilawood essential oil extracts residue 77~87%, wheat bran 10~20%, lime 2.00% and gypsum 1.00%.The present invention extracts residue as main compost cultivating ganoderma using agilawood essential oil, the nutritional ingredient of agalloch eaglewood can be fully absorbed, agalloch eaglewood medicinal health health effect is transferred to ganoderma lucidum, to further promote the health functions of ganoderma lucidum, improves the planting benefit of agalloch eaglewood and ganoderma lucidum.Further extracting residue dosage present invention defines agilawood essential oil makes the growth of cultivating ganoderma and biological transformation ratio all preferable.
Description
Technical field
It is the present invention relates to a kind of Medium for Ganoderma lucidum and preparation method thereof, in particular to a kind of to be with agilawood essential oil extraction residue
Medium for Ganoderma lucidum of major ingredient and preparation method thereof.
Background technique
Agalloch eaglewood is the resin generated after suspension culture of Aquilaria sinensis tree old stem is injured, is mixed with consolidating for resin (grease) ingredient and wood substance component
State condensation product is commonly called as.Suspension culture of Aquilaria sinensis is used as medicine with resiniferous timber, is certified products source and the domestic product of domestic Chinese medicine agalloch eaglewood
Unique plant resources of Chinese medicine agalloch eaglewood.The agalloch eaglewood that suspension culture of Aquilaria sinensis is formed is known as buta-buta or Chinese agalloch eaglewood, and it is long-drawn-out to be that China continues to use history
Long precious Chinese medicine and fragrance.Agalloch eaglewood is widely used, in addition to for Chinese medicine, is also used to fragrance and craftwork engraving, in recent years
Agalloch eaglewood value rises violently, and reaches the degree of " a piece of ten thousand gold medal ".Agalloch eaglewood medicinal material market demand is big, but the period formed is long, natural wild
It increasingly reduces in production-goods source.As protection of resources and meet the counter-measure of the market demand, the ground such as Guangdong, Guangxi, Hainan are big at present
Wide artificial growth agallochum is pushed, plantation amount increases substantially year by year.
Magical effect of agalloch eaglewood is mostly derived from agalloch eaglewood containing sesquiterpenoids and chromone compounds, both at
Divide and is primarily present in agalloch eaglewood volatile oil.At present in addition to the wood artwork of part agalloch eaglewood, most of agalloch eaglewood product need to be by agalloch eaglewood
Essential oil is extracted from agalloch eaglewood xylem and is processed again.Conventional agilawood essential oil extracting method have steam distillation,
95% alcohol reflux extraction process, Benzene-ethanol extraction method, petroleum ether infusion process etc., and extraction by steam distillation agilawood essential oil is simply held
Easy to operate, at low cost, the essential oil quality produced is preferable.After agilawood essential oil high-volume output, residue treatment is asked after extraction of essential oil
Topic receives significant attention.
Ganoderma lucidum is the drying fructification of On Polyporaceae red sesame or purple sesame, is a kind of medicine of preciousness in China's medical treasure-house
Edible mushroom.The conventional production methods of ganoderma lucidum are broad-leaved cultivation basswood, as " bacterium woods contradiction " is prominent, " wildwood protected project "
Implement and the emergence and development of Juncao technology, the production of ganoderma lucidum have turned to substituting stuff cultivation be leading.Currently, weed tree sawdust, cotton
Sub- shell, ramulus mori, corncob etc. are used ganoderma lucidum generation material production, have saved a large amount of linden resource, have effectively alleviated " bacterium woods lance
It shield " and protects forest resources.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without answering
When being considered as recognizing or imply that the information constitutes the prior art already known to those of ordinary skill in the art in any form.
Summary of the invention
Residue is difficult after the present invention is extracted for agilawood essential oil in the prior art, and processing needs cost, agalloch eaglewood processing surplus
The problems such as excess resource utilization rate is low is invented a kind of using agilawood essential oil extraction residue as the Medium for Ganoderma lucidum of major ingredient and its preparation
Method, it is intended to obtain one kind using agilawood essential oil and extract residue as main compost cultivating ganoderma, by agalloch eaglewood medicinal health health
Effect is transferred to ganoderma lucidum, to promote the Medium for Ganoderma lucidum of the health functions of ganoderma lucidum.
To achieve the above object, technical solution provided by the invention is as follows:
It is a kind of that residue is extracted as the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, include following components according to mass percent:
Agilawood essential oil extracts residue 77~87%, wheat bran 10~20%, lime 2.00% and gypsum 1.00%.
Preferably, it is agalloch eaglewood whitewood through crushing, extraction by steam distillation agalloch eaglewood that the agilawood essential oil, which extracts residue,
Remaining residue after essential oil is dried to gained after mass water content≤20%.
Preferably, described that residue is extracted as the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, include according to mass percent
Following components: agilawood essential oil extracts residue 77%, wheat bran 20.00%, lime 2.00% and gypsum 1.00%.
It is a kind of that residue is extracted as the preparation method of the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, include following operating procedure:
(1) weigh following components according to mass percent: agilawood essential oil extract residue 77~87%, wheat bran 10~20%,
Then agilawood essential oil is extracted residue, wheat bran, lime and gypsum and is mixed evenly, added by lime 2.00% and gypsum 1.00%
Water adjusts gained culture base-material water content, and adjusting pH value by the lime in ingredient is 7.5；
(2) compost obtained by step (1) is packed, ties sack, sterilizing extracts residue as major ingredient to get using agilawood essential oil
Medium for Ganoderma lucidum.
Preferably, gained compost mass water content is 60~65% in step (1) plus after water management mixing.
Compared with prior art, the invention has the following beneficial effects:
The present invention extracts residue as main compost cultivating ganoderma using agilawood essential oil, can fully absorb the nutrition of agalloch eaglewood at
Point, agalloch eaglewood medicinal health health effect is transferred to ganoderma lucidum, to further promote the health functions of ganoderma lucidum, improves agalloch eaglewood
With the planting benefit of ganoderma lucidum.Further present invention defines agilawood essential oil extract residue dosage make cultivating ganoderma growth and
Biological transformation ratio is all preferable.
Specific embodiment
It is described in detail With reference to embodiment, it is to be understood that protection scope of the present invention is not by specific
The limitation of embodiment.Agilawood essential oil used in the examples extracts residue and comes from Guangxi Bobai County agalloch eaglewood timber processing space, by
Agalloch eaglewood whitewood remaining residue after crushing, extraction by steam distillation agilawood essential oil, is dried spare；
Remaining timber after the agalloch eaglewood sawdust used is felled for agalloch eaglewood whitewood, carves silk, using the sawdust for crushing formation；
The red ganoderma lucidum of strains tested is provided by institute of microbiology of Guangxi Academy of Agricultural Sciences breed of edible fungus center；
Other raw and auxiliary materials are commercially available gained.
Embodiment 1
A kind of to extract residue as the preparation method of the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, operating procedure is as follows:
(1) weigh following components according to mass percent: above-mentioned spare agilawood essential oil extracts residue 87%, wheat bran
10%, then agilawood essential oil is extracted residue, wheat bran, lime and stone by lime 2.00% and gypsum 1.00%, total material dry weight 25kg
Cream is mixed evenly, and adds water, and gained compost mass water content is 60~65% after control mixing, passes through the lime in ingredient
Adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, every bag of total material dry weight 25kg, ties sack, sterilized to get with agalloch eaglewood
Extraction of essential oil residue is the Medium for Ganoderma lucidum of major ingredient.
Embodiment 2
A kind of to extract residue as the preparation method of the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, operating procedure is as follows:
(1) weigh following components according to mass percent: above-mentioned spare agilawood essential oil extracts residue 82%, wheat bran
15%, then agilawood essential oil is extracted residue, wheat bran, lime and stone by lime 2.00% and gypsum 1.00%, total material dry weight 25kg
Cream is mixed evenly, and adds water, and gained compost mass water content is 60~65% after control mixing, passes through the lime in ingredient
Adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, every bag of total material dry weight 25kg, ties sack, sterilized to get with agalloch eaglewood
Extraction of essential oil residue is the Medium for Ganoderma lucidum of major ingredient.
Embodiment 3
A kind of to extract residue as the preparation method of the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, operating procedure is as follows:
(1) weigh following components according to mass percent: above-mentioned spare agilawood essential oil extracts residue 77%, wheat bran
20%, then agilawood essential oil is extracted residue, wheat bran, lime and stone by lime 2.00% and gypsum 1.00%, total material dry weight 25kg
Cream is mixed evenly, and adds water, and gained compost mass water content is 60~65% after control mixing, passes through the lime in ingredient
Adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, every bag of total material dry weight 25kg, ties sack, sterilized to get with agalloch eaglewood
Extraction of essential oil residue is the Medium for Ganoderma lucidum of major ingredient.
Comparative example 1
A kind of preparation method of Medium for Ganoderma lucidum, operating procedure are as follows:
(1) following components is weighed according to mass percent: agalloch eaglewood sawdust 77%, wheat bran 20%, lime 2.00% and gypsum
Then 1.00%, total material dry weight 25kg agalloch eaglewood sawdust, wheat bran, lime and gypsum are mixed evenly, add water, control mixing
Gained Compost moisture content is 60~65% afterwards, and adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, every bag of total material dry weight 25kg, ties sack, sterilizing to get.
Comparative example 2
A kind of preparation method of Medium for Ganoderma lucidum, operating procedure are as follows:
(1) following components is weighed according to mass percent: weed tree sawdust 77%, wheat bran 20%, lime 2.00% and gypsum
Then 1.00%, total material dry weight 25kg weed tree sawdust, wheat bran, lime and gypsum are mixed evenly, add water, after control mixing
Gained Compost moisture content is 60%, and adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, every bag of total material dry weight 25kg, ties sack, sterilizing to get.
Service test
1, the various embodiments described above 1~3, the preparation gained culture medium temperature of comparative example 1~2 are dropped to 28~30 DEG C, i.e.,
It can be inoculated with, i.e., be tested to each red ganoderma lucidum of inoculation of medium of preparation gained, each embodiment preparation gained cultivates base weight
It is 3 times multiple:
(a) it is inoculated with, every bag of inoculum concentration is 20% of culture medium quality obtained by above-mentioned preparation；
(b) bacterium bag: being placed in culturing room after inoculation and be protected from light culture according to a conventional method by Mycelium culture, and room temperature is controlled 25
~28 DEG C, relative air humidity is 65%~70%；Miscellaneous bacteria will be infected if there is living contaminants in time by observing hypha growth condition
Bacterium bag pick elimination, in order to avoid infect other bacterium bags；
(c) go out sesame management: conventional method
(d) harvest: when ganoderma lucidum cap no longer increases, edge white circle disappears, while dispersing with a large amount of Reishi sporule
When, it is the mark of ganoderma lucidum fruitbody maturation, should harvests at this time, two damp ganoderma lucidum fruitbodies is harvested altogether, by ganoderma lucidum fruitbody in 60 DEG C
Drying to constant weight, the analysis for yield and quality.
2, mycelium length and biological efficiency observation are noted down
Using simple interest mensuration record Mycelium Growth of Ganoderma lucidum speed in each culture medium prescription (day growth rate mm/d),
The hypha growth condition of different disposal (is compared to each other respectively, indicates mycelial density and life with "+" number by mycelial growth potential and density
Growing way is strong and weak, and "+" more multilist shows that mycelial density is bigger and growth potential is stronger).Management of producing mushroom carries out according to a conventional method with harvesting, remembers
Record counts the yield of two damp mushrooms before each formula, calculation biology efficiency.
Average daily growth rate=mycelium morphology factor/the cultivated days of mycelia；
Biological efficiency (%)=(dry mushroom weight/siccative weight) × 100.
Then significant difference analysis is carried out using duncan's new multiple range method, obtains data shown in Tables 1 and 2:
The mycelial growth situation of ganoderma lucidum on each embodiment culture medium of table 1
Note: being illustrated respectively in 0.01 and 0.05 horizontal upper significant difference with the mother that writes of different size after column of figure in table 1, under
Together.
++ indicate that mycelium growth vigor is strong, it is sturdy, pure white, neat；+ indicating that mycelium growth vigor is stronger, sturdy, pure white but growth is not
It is enough neat.
As shown in Table 1, each embodiment preparation gained culture medium is suitable for Mycelium Growth of Ganoderma lucidum, but the length of each culture medium ganoderma lucidum
Gesture, long speed are different:
Mycelium growth vigor: it is certain poor that growth potential, color, density of the Ganoderma lucidum mycelium on the culture medium obtained by the preparation of each embodiment have
Different: mycelia is more sparse on 1 gained culture medium of embodiment, color is partially light, and growing way is slightly weak compared with other culture mediums；And other four
Ganoderma lucidum mycelium all shows preferable growth potential on a culture medium, and mycelia growth is vigorous, front end is neat, pure white dense, sturdy has
Power.
Mycelium length: obtained by the preparation of each embodiment in culture medium, 1 gained culture medium Mycelium Growth of Ganoderma lucidum of embodiment most
Slowly, the per day speed of growth is 8.7mm, extremely significant to be slower than other formulas；And Ganoderma lucidum mycelium is per day on other four culture mediums
The speed of growth is not significant in 0.05 and 0.01 horizontal upper difference.Illustrate that the content of the wheat bran in culture medium prescription grows mycelia
It is affected, in a certain range, content is higher, and Mycelium Growth of Ganoderma lucidum is faster.
Ganoderma lucidum volume variance conspicuousness compares on each embodiment culture medium of table 2
As shown in Table 2, in the culture medium obtained by the preparation of each embodiment, ganoderma lucidum average product is with 3 gained culture medium of embodiment
Highest, dry product ganoderma lucidum biological efficiency is up to 6.32%, significant with other formula variations in 0.05 level, in 0.01 level
Except with 2 gained culture medium difference of embodiment it is not significant in addition to, it is significant with other formula variations；Followed by 2 gained culture medium of embodiment,
Dry product ganoderma lucidum biological efficiency is 5.80%, 0.05 it is horizontal upper and 0.01 it is horizontal above and 1 gained culture medium difference of embodiment not
Significantly, but it is significantly higher than 2 gained culture medium of 1 gained culture medium of comparative example and comparative example；Lower yield is comparison
2 gained culture medium of 1 gained culture medium of embodiment and comparative example, difference between the two is not significant, cultivates with obtained by embodiment 1
Basis is not different significant.Illustrate that agilawood essential oil extracts the good raw material that residue is cultivating ganoderma, wheat bran additive amount properly exists in use
When 15-20%, ganoderma lucidum yield is significantly higher than agalloch eaglewood sawdust (1 gained culture medium of comparative example) not through processing and general
Weed tree sawdust (5 gained culture medium of comparative example), and agalloch eaglewood sawdust not through processing and the cultivation spirit of general weed tree sawdust
Sesame effect is the same, and volume variance is not significant.
Agilawood essential oil extracts residue and refers to that agilawood essential oil is passed through the methods of steam distillation from agalloch eaglewood xylem to be mentioned
Residue after taking out, since it must just be crushed before extraction, and fineness degree is precisely required by ganoderma lucidum substituting stuff cultivation,
It can be reduced when being accordingly used in cultivation of glossy ganoderma and crush this program.In addition, lasting steam high temperature makes in agilawood essential oil extraction process
The effective component of agalloch eaglewood sawdust is degraded, while having been killed and largely having been grown unfavorable miscellaneous bacteria to ganoderma lucidum, and spirit is not only advantageous to
Sesame growth, and reduce fermenting procedure in general cultivation of glossy ganoderma, not only saved the time but also saved cost.
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and its actually answering
With so that those skilled in the art can be realized and utilize a variety of different exemplary implementation schemes of the invention and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (5)
1. a kind of extract residue as the Medium for Ganoderma lucidum of major ingredient using agilawood essential oil, which is characterized in that include according to mass percent
Following components: agilawood essential oil extracts residue 77~87%, wheat bran 10~20%, lime 2.00% and gypsum 1.00%.
2. Medium for Ganoderma lucidum according to claim 1, it is characterised in that: it is that agalloch eaglewood is white that the agilawood essential oil, which extracts residue,
Wood remaining residue after crushing, extraction by steam distillation agilawood essential oil is dried to gained after water content≤20%.
3. Medium for Ganoderma lucidum according to claim 1, it is characterised in that: described to extract residue as major ingredient using agilawood essential oil
Medium for Ganoderma lucidum, include following components according to mass percent: agilawood essential oil extracts residue 77%, wheat bran 20.00%, lime
2.00% and gypsum 1.00%.
4. the preparation method of Medium for Ganoderma lucidum as described in claim 1, which is characterized in that include following operating procedure:
(1) weigh following components according to mass percent: agilawood essential oil extracts residue 77~87%, wheat bran 10~20%, lime
2.00% and gypsum 1.00%, agilawood essential oil is then extracted into residue, wheat bran, lime and gypsum and is mixed evenly, water tune is added
Section gained culture base-material water content, adjusting pH value is 7.5；
(2) compost obtained by step (1) is packed, sterilizing extracts residue as the Medium for Ganoderma lucidum of major ingredient to get using agilawood essential oil.
5. the preparation method according to claim 4, it is characterised in that: gained is cultivated in step (1) plus after water management mixing
Expect that water content is 60~65%.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960159A (en) * | 2012-11-28 | 2013-03-13 | 西安福安创意咨询有限责任公司 | Planting method for producing agricultural products containing Aquilaria sinensis |
| TWI577797B (en) * | 2013-04-11 | 2017-04-11 | Studies on the Culture Methods and Culture Substrate of | |
| CN107353060A (en) * | 2017-07-13 | 2017-11-17 | 深圳前海珍稀沉香生物科技有限公司 | Mushroom culture composition, agalloch eaglewood mushroom and agalloch eaglewood mushroom cultural method |
| CN107466677A (en) * | 2017-08-22 | 2017-12-15 | 张桂清 | A kind of method of cedar sawdust cultivating ganoderma |
| CN108076963A (en) * | 2017-10-26 | 2018-05-29 | 贵州大学 | A kind of red sesame culture medium and preparation method thereof |
-
2018
- 2018-10-17 CN CN201811208424.1A patent/CN109220548A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960159A (en) * | 2012-11-28 | 2013-03-13 | 西安福安创意咨询有限责任公司 | Planting method for producing agricultural products containing Aquilaria sinensis |
| TWI577797B (en) * | 2013-04-11 | 2017-04-11 | Studies on the Culture Methods and Culture Substrate of | |
| CN107353060A (en) * | 2017-07-13 | 2017-11-17 | 深圳前海珍稀沉香生物科技有限公司 | Mushroom culture composition, agalloch eaglewood mushroom and agalloch eaglewood mushroom cultural method |
| CN107466677A (en) * | 2017-08-22 | 2017-12-15 | 张桂清 | A kind of method of cedar sawdust cultivating ganoderma |
| CN108076963A (en) * | 2017-10-26 | 2018-05-29 | 贵州大学 | A kind of red sesame culture medium and preparation method thereof |
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