CN109220497A - A kind of cuttage breeding method of Cortex Ilicis Rotundae - Google Patents

A kind of cuttage breeding method of Cortex Ilicis Rotundae Download PDF

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CN109220497A
CN109220497A CN201811376632.2A CN201811376632A CN109220497A CN 109220497 A CN109220497 A CN 109220497A CN 201811376632 A CN201811376632 A CN 201811376632A CN 109220497 A CN109220497 A CN 109220497A
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matrix
cuttage
cutting
root
cortex ilicis
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张洺瑄
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00—Vegetative propagation
    • A01G2/10—Vegetative propagation by means of cuttings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23—Wood, e.g. wood chips or sawdust
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings

Abstract

The invention belongs to forest genetics technical fields, and in particular to a kind of cuttage breeding method of Cortex Ilicis Rotundae.Daily management and acclimatization and transplants after this method includes acquisition cutting, substrate preparation, matrix and slotting machine disinfection treatment, cuttage, inserts.It joined 40% decomposed cow dung in matrix used in the present invention, CO generated by the decomposition of organic matter2, while increasing CO2Gas is fertile and increases the CO in soil and air using the measure of high-pressure sodium lamp light filling2Concentration and intensity of illumination reinforce photosynthesis, promote the more sugar of cutting accumulation and root-promoting hormone to shift to base portion, to improve rooting rate and root system quality.Present invention optimizes the composition proportion of root-promoting hormone and dosages, have not only guaranteed rooting of cuttings rate and quality, but also effectively reduce expenses, have reduced unnecessary consumption.The method of the present invention has the advantages that science, effective, strong operability, rooting of cuttings rate can be made 81.5% or more, next year, survival rate was 74.6% or more.

Description

A kind of cuttage breeding method of Cortex Ilicis Rotundae
Technical field
The invention belongs to forest genetics technical fields, and in particular to a kind of cuttage breeding method of Cortex Ilicis Rotundae.
Background technique
Cortex Ilicis Rotundae (Ilex latifolia Thunb.) is that Aquifoliaceae (Aquifoliaceae) Ilex (Ilex) is normal Green broad-leaved arbor is NATURAL DISTRIBUTION in a kind of the green of regional preciousness such as China middle and lower reach of Yangtze River Sichuan, Jiangxi, Zhejiang, Anhui Change tree species.According to records, also there is a small amount of NATURAL DISTRIBUTION in the domestic Dabie Mountain in Nanyang City, Henan Province, Funiu Shan Mountain, Tongbai Shan Mountain.Great Ye Chinese ilex trunk is logical straight, and tree-like grace is with luxuriant foliage and spreading branches in leafy profusion, exactly likes wide yulan.1 year middle period, flower, the variation of fruit form and aspect are abundant, very beautiful It sees.Compendium of Material Medica is recorded, and Cortex Ilicis Rotundae is bitter in taste, puckery, cold, there is clearing heat and detoxicating, relieve heat, the function of promoting blood circulation and hemostasis, is domestic Make the important source material of Ilex Latifolia Thunb green plants health-care agent.Cortex Ilicis Rotundae collects ornamental greening, medicine healthy sofa and excellent use material in one Body is a kind of rare tree rare in urban afforestation.
With gradual warming up for global climate, for the relatively deficient north city of evergreen species, Cortex Ilicis Rotundae Exploitation, using being also increasingly taken seriously.Meanwhile serious hope and high-quality natural health drink of the people to improving the ecological environment --- The demand of Ilex Latifolia Thunb is also increasing.But in production practices, since Cortex Ilicis Rotundae conventional manual reproduction technique more falls behind, i.e., Make also only have a small amount of elite stand in the natural Proterozoic such as Zhe Nan.Cortex Ilicis Rotundae system deep dormancy tree species, conventional seeding and seedling raising seed Germination percentage is often less than 30% (Li Xiao storage, the latifolia Thunb Cortex Ilicis Rotundae introduction and Experiment preliminary study such as Huang Libin, Zhu Xichen [J], Jiangsu forestry science and technology, 2003,30 (5): 1-6.)；It can be taken root using cuttings when cutting propagation, but rooting rate is often less than 70%, and most of seedling often wither because of environment discomfort (China of Zhu state Cortex Ilicis Rotundae shoot cutting comparative test preliminary study [J], Zhejiang Forest science and technology, 2004,24 (4): 21-24.) (cutting propagation and study on drought resistance [D] of Chen Wei Ilex several plant, Nanjing, Nanjing Forestry University, 2006.).In addition, the difficulties such as merit of seeding and seedling raising elite stand, especially medicinal ingredient and resistance Equably to obtain heredity in offspring, to not can guarantee the standardization of Cortex Ilicis Rotundae merit and effective medicinal ingredient.
Summary of the invention
The present invention is directed to that the emergence rate occurred in traditional Cortex Ilicis Rotundae reproductive process is low, seedling stage adaptability ability is poor, Optimality The problems such as shape Genomic instability, obtains the method for breeding a large amount of choiceness plant, rooting of cuttings rate can be made 81.5% More than, the nursery stock of breeding is healthy and strong, adaptability ability is strong, and next year, survival rate was 74.6% or more.Conscientiously the number of breeding nursery stock is improved Amount and quality, are the important breakthroughs of Cortex Ilicis Rotundae tradition reproduction technique, realize the target for obtaining more choiceness.
To achieve the above object, the invention adopts the following technical scheme:
A kind of cuttage breeding method of Cortex Ilicis Rotundae, specifically includes the following steps:
Step 1: acquisition cutting: choosing health, no disease and pests harm, axillary bud full current year raw semi-lignified branch June~mid-July Item goes terminal bud to be cut into the cutting of long 8~10cm；2~3 axillary buds of reservation in cutting, upper cut truncation, 45 degree of lower cut bevelings, Upper and lower notch is away from nearest axillary bud 0.5cm or more；Retain 1~2, top leaf, every leaf retains 1/3~1/2.Lower cut and armpit The distance of bud refers to the top of lower cut at a distance from axillary bud.
Cutting is with adopting with if you need to save transport, taking cooling, moist keeping measures with before depositing in shady place.
Step 2: the preparation of matrix: according to quality than sawdust: sandy loam: fertile soil: decomposed cow dung is 1:1:4:4 or saw End: fertile soil: decomposed cow dung is the ratio mixed raw material of 1:5:4, prepares matrix；
Step 3: matrix, slotting machine disinfection treatment: cuttage the last week carries out 10~20cm to slotting machine and turns over, with 0.5% permanganic acid Potassium carries out disinfection；It mixes proportioned matrix thoroughly sieving, the 50% carbendazim wettable powder of 50g is mixed in every cube of matrix Agent is covered 3~4 days with film, takes off film；The matrix disinfected is packed into nutrition cup by the 1d before cuttage；
Step 4: cuttage: selection of time is in June~mid-July；
Growth-promoting root processing: indolebutyric acid and methyl α-naphthyl acetate are configured to the root-promoting hormone aqueous solution of 1000mg/kg；By the quickly dipped life of cutting Root hormone aqueous solution, dipping the time is 5~8 seconds；
With small wooden, Zha Dong, the depth in hole are 4~5cm, the cutting cuttage that will have been dipped with root-promoting hormone in matrix when cuttage Into matrix, compacting, temperature and relative air humidity are debugged in spray water immediately moisturizing, heat preservation；
Step 5: daily management after inserting:
Humidity: keeping leaf wetness using automatic sprayer, and spray head full diameter is the fog spray-head of 2mm, keeps relative humidity 95% or more；
Increase CO2Gas fertilizer: every morning 9~11 point use CO2Occurrence of equipment increases CO2Gas fertilizer；
Supplementary light: daily high-pressure sodium lamp light filling 5h, 6~8 high-pressure sodium lamp light fillings 2h, 16~19 high-pressure sodium lamp light filling 3h；
Sterilizing: 1 800~1000 times of diluted 50% carbendazol wettable powder or methyl support are alternately sprayed every 15d Cloth saliva fungicide；Period if any phenomena such as drying up, going mouldy will remove in time, is changed to spray 1 time every 20d after rooting of cuttings；
Step 6: hardening transplanting:
After culture 2.5~3 months, hardening is carried out in greenhouse；Greenhouse is maintained at 20~28 DEG C during hardening, humidity 80~90%, After 1~3 times/day, 3 months of ventilation, the above whole day of height of seedling 25cm opens ceiling, and spring next year band nutrition cup moves on to outdoor or fixed It plants.
Further, the compound diammonium hydrogen phosphate fertilizer that mass percent is not more than 2% is additionally added in the matrix.
Further, in step 4, the temperature is 25~30 DEG C, and relative air humidity is 80~95%.
Further, length of the cutting cuttage into matrix is the 1/2 of cutting overall length.
Further, the root-promoting hormone is mixed by indolebutyric acid IBA and methyl α-naphthyl acetate NAA according to mass ratio 3:1.
Compared with prior art, the beneficial effects of the present invention are:
(1) it joined 40% decomposed cow dung in matrix, CO generated by the decomposition of organic matter2, while increasing CO2Gas fertilizer and use High-pressure sodium lamp light filling measure increases the CO in soil and air2Concentration and intensity of illumination reinforce photosynthesis, cutting are promoted to accumulate More sugar and root-promoting hormone are shifted to base portion, to improve rooting rate and root system quality.
(2) composition proportion and dosage for optimizing root-promoting hormone had not only guaranteed rooting of cuttings rate and quality, but also have effectively saved and open Branch, reduces unnecessary consumption.
(3) it is directed to Cortex Ilicis Rotundae and breeds feature that is difficult, surviving hardly possible, this method has scientific, effective, strong operability excellent Point can make rooting of cuttings rate 81.5% or more, and next year survival rate is 74.6% or more, and the nursery stock of breeding is healthy and strong, adaptability ability By force, the more high-quality clonal targets of acquisition are realized.
Specific embodiment
The following examples are intended to illustrate the invention, but is not used to limit the scope of protection of the present invention.Unless otherwise specified, real Apply the conventional means that technological means used in example is well known to those skilled in the art.
Refer to the top of lower cut at a distance from axillary bud at a distance from lower cut and axillary bud in embodiment.
The 1 different acquisition time of embodiment, different degree of lignification, different length cutting cuttage survival rate
Step 1: acquisition cutting: it is tender to choose health, no disease and pests harm, axillary bud full current year raw terminal bud, semi-lignified April~September Branch or mature spray, are cut into the cutting of long 8~10cm, 10cm or 10cm or more；
Retain 2~3 axillary buds in cutting, upper and lower notch is away from axillary bud 0.5cm or more, and upper cut truncation, 45 degree of lower cut are tiltedly It cuts；Retain 1~2 leaf of top, every leaf retains 1/3~1/2.Cutting is with before depositing in shady place, with adopting with if you need to save fortune It is defeated, to take cooling, moist keeping measures.
Step 2: the preparation of matrix: according to quality than sawdust: sandy loam: fertile soil: decomposed cow dung is that 1:1:4:4 prepares base Then the compound diammonium hydrogen phosphate fertilizer of mass percent 2% is added in matter in matrix.
Step 3: matrix, slotting machine disinfection treatment: cuttage the last week carries out 10~20cm to slotting machine and turns over, high with 0.5% Potassium manganate carries out disinfection；Proportioned matrix mixes 50% carbendazol wettable powder, every 1 cubic metre of Medium Culture after mixing sieving thoroughly 50 gram of 50% carbendazol wettable powder uniformly is admixed, is covered 3~4 days with film, takes off film.The 1d before cuttage, by what is disinfected Matrix is packed into nutrition cup, puts in warm canopy neat.Start to debug temperature, relative air humidity, makes 25~30 DEG C of temperature, sky Gas relative humidity is 80~95%.
Step 4: cuttage: growth-promoting root processing: the root-promoting hormone that indolebutyric acid and methyl α-naphthyl acetate are configured to 1000mg/kg is water-soluble Liquid；By the quickly dipped root-promoting hormone aqueous solution of cutting, dipping the time is 5~8 seconds；Draw diindyl butyric acid and methyl α-naphthyl acetate in root-promoting hormone aqueous solution Mass ratio be 3:1.
With small wooden, Zha Dong, the depth in hole are 4~5cm, the cutting that will have been dipped with root-promoting hormone in matrix when cuttage Into matrix, the length of insertion is the 1/2 of cutting length for cuttage, compacting, spray water immediately moisturizing, heat preservation.
Step 5: daily management after inserting:
Humidity: keeping leaf wetness using automatic sprayer, and spray head full diameter is the fog spray-head of 2mm, keeps relative humidity 95% or more；
Increase CO2Gas fertilizer: every morning 9~11 point use CO2Occurrence of equipment increases CO2Gas fertilizer；
Supplementary light: daily high-pressure sodium lamp light filling 5h, 6~8 high-pressure sodium lamp light fillings 2h, 16~19 high-pressure sodium lamp light filling 3h；
Sterilizing: 1 800~1000 times of diluted 50% carbendazol wettable powder or methyl support are alternately sprayed every 15d Cloth saliva fungicide；Period if any phenomena such as drying up, going mouldy will remove in time, is changed to spray 1 time every 20d after rooting of cuttings.
Step 6: hardening transplanting:
After culture 2.5~3 months, hardening is carried out in greenhouse；Greenhouse is maintained at 20~28 DEG C during hardening, humidity 80~90%, After 1~3 times/day, 3 months of ventilation, the above whole day of height of seedling 25cm opens ceiling, and spring next year band nutrition cup moves on to outdoor or fixed It plants.
The 1 different acquisition time of table, different degree of lignification, different length cutting cuttage survival rate
The result shows that: the current year life semi-lignified rooting of cuttings rate and next year survival rate that June~July acquires are most Secondly height is the lower cutting of degree of lignification of same time acquisition.Current year, raw semi-lignified rooting of cuttings rate was 81.5%, secondary Year, survival rate was 74.6%, and compared with the lower rooting of cuttings rate raising 23.3% of same period degree of lignification, next year survival rate is improved 132.4%；Height and rugosity can reach requirement after current year raw semi-lignified cutting survives, and ratio of height to diameter is minimum, illustrates seedling The high-quality stalwartness of wood.Therefore it selects in June~acquisition in July Cortex Ilicis Rotundae current year raw semi-lignified branch to be cutting, carries out cuttage Breeding.
The cuttage survival rate of 2 different substrates type of embodiment and the cutting under proportion
Step 1: acquisition cutting: choosing health, no disease and pests harm, axillary bud full current year raw semi-lignified branch June~mid-July Item goes terminal bud to be cut into the cutting of long 8~10cm；Retain 2~3 axillary buds in cutting, upper and lower notch away from axillary bud 0.5cm or more, on Notch truncation, 45 degree of lower cut bevelings；Retain 1~2, top leaf, every leaf retains 1/3~1/2；Cutting is with before depositing in yin Liang Chu, with adopt with, if you need to save transport, to take cooling, moist keeping measures.
Step 2: the preparation of matrix: being prepared according to 6 kinds of matrix types in table 2.
Step 3: matrix, slotting machine disinfection treatment: cuttage the last week carries out 10~20cm to slotting machine and turns over, high with 0.5% Potassium manganate carries out disinfection；Proportioned matrix mixes 50% carbendazol wettable powder, every 1 cubic metre of Medium Culture after mixing sieving thoroughly 50 gram of 50% carbendazol wettable powder uniformly is admixed, is covered 3~4 days with film, takes off film.The 1d before cuttage, by what is disinfected Matrix is packed into nutrition cup, puts in warm canopy neat.Start to debug temperature, relative air humidity, makes 25~30 DEG C of temperature, sky Gas relative humidity is 80~95%.
Step 4: cuttage: growth-promoting root processing: the root-promoting hormone that indolebutyric acid and methyl α-naphthyl acetate are configured to 1000mg/kg is water-soluble Liquid；By the quickly dipped root-promoting hormone aqueous solution of cutting, dipping the time is 5~8 seconds；Draw diindyl butyric acid and methyl α-naphthyl acetate in root-promoting hormone aqueous solution Mass ratio be 3:1.
With small wooden, Zha Dong, the depth in hole are 4~5cm, the cutting that will have been dipped with root-promoting hormone in matrix when cuttage Into matrix, the length of insertion is the 1/2 of cutting length for cuttage, compacting, spray water immediately moisturizing, heat preservation.
Step 5: daily management after inserting:
Humidity: keeping leaf wetness using automatic sprayer, and spray head full diameter is the fog spray-head of 2mm, keeps relative humidity 95% or more；
Increase CO2Gas fertilizer: every morning 9~11 point use CO2Occurrence of equipment increases CO2Gas fertilizer；
Supplementary light: daily high-pressure sodium lamp light filling 5h, 6~8 high-pressure sodium lamp light fillings 2h, 16~19 high-pressure sodium lamp light filling 3h；
Sterilizing: 1 800~1000 times of diluted 50% carbendazol wettable powder or methyl support are alternately sprayed every 15d Cloth saliva fungicide；Period if any phenomena such as drying up, going mouldy will remove in time, is changed to spray 1 time every 20d after rooting of cuttings.
Step 6: hardening transplanting:
After culture 2.5~3 months, hardening is carried out in greenhouse；Greenhouse is maintained at 20~28 DEG C during hardening, humidity 80~90%, After 1~3 times/day, 3 months of ventilation, the above whole day of height of seedling 25cm opens ceiling, and spring next year band nutrition cup moves on to outdoor or fixed It plants.
2 cutting medium type of table and proportion
The result shows that: in No. 2 matrix based on detritus soil and decomposed cow dung, while a small amount of sandy loam and sawdust are matched, it is raw Root rate has dropped 8.32% compared with the 5th scheme, but next year survival rate improves 100.54% or more, and ratio of height to diameter is significantly reduced, and is said The nursery stock of bright breeding is healthy and strong, and adaptability ability is stronger.Secondly be No. 4 matrix, rooting rate and next year survival rate also it is relatively high simultaneously And stablize.Therefore optimal matrix is 2 and 4, i.e., with sawdust: fertile soil: decomposed cow dung is 1:5:4 or sawdust: sandy loam: humic Soil: the compound diammonium hydrogen phosphate fertilizer of 2% (mass percent) is added in decomposed cow dung 1:1:4:4.
The cuttage survival rate of cutting under the different root-promoting hormone formulas of embodiment 3
Step 1: acquisition cutting: choosing health, no disease and pests harm, axillary bud full current year raw semi-lignified branch June~mid-July Item goes terminal bud to be cut into the cutting of long 8~10cm；Retain 2~3 axillary buds in cutting, upper and lower notch away from axillary bud 0.5cm or more, on Notch truncation, 45 degree of lower cut bevelings；Retain 1~2, top leaf, every leaf retains 1/3~1/2；Cutting is with before depositing in yin Liang Chu, with adopt with, if you need to save transport, to take cooling, moist keeping measures.
Step 2: the preparation of matrix: according to quality than sawdust: sandy loam: fertile soil: decomposed cow dung is that 1:1:4:4 prepares base Then the compound diammonium hydrogen phosphate fertilizer of mass percent 2% is added in matter in matrix.
Step 3: matrix, slotting machine disinfection treatment: cuttage the last week carries out 10~20cm to slotting machine and turns over, high with 0.5% Potassium manganate carries out disinfection；Proportioned matrix mixes 50% carbendazol wettable powder, every 1 cubic metre of Medium Culture after mixing sieving thoroughly 50 gram of 50% carbendazol wettable powder uniformly is admixed, is covered 3~4 days with film, takes off film.The 1d before cuttage, by what is disinfected Matrix is packed into nutrition cup, puts in warm canopy neat.Start to debug temperature, relative air humidity, makes 25~30 DEG C of temperature, sky Gas relative humidity is 80~95%.
Step 4: it is water-soluble growth-promoting root processing: cuttage: to prepare root-promoting hormone according to root-promoting hormone type shown in table 3 and concentration Liquid；By the quickly dipped root-promoting hormone aqueous solution of cutting, dipping the time is 5~8 seconds.
With small wooden, Zha Dong, the depth in hole are 4~5cm, the cutting that will have been dipped with root-promoting hormone in matrix when cuttage Into matrix, the length of insertion is the 1/2 of cutting length for cuttage, compacting, spray water immediately moisturizing, heat preservation.
Step 5: daily management after inserting:
Humidity: keeping leaf wetness using automatic sprayer, and spray head full diameter is the fog spray-head of 2mm, keeps relative humidity 95% or more；
Increase CO2Gas fertilizer: every morning 9~11 point use CO2Occurrence of equipment increases CO2Gas fertilizer；
Supplementary light: daily high-pressure sodium lamp light filling 5h, 6~8 high-pressure sodium lamp light fillings 2h, 16~19 high-pressure sodium lamp light filling 3h； Sterilizing: 1 800~1000 times of diluted 50% carbendazol wettable powder or thiophanate methyl are alternately sprayed every 15d Fungicide；Period if any phenomena such as drying up, going mouldy will remove in time, is changed to spray 1 time every 20d after rooting of cuttings.
Step 6: hardening transplanting:
After culture 2.5~3 months, hardening is carried out in greenhouse；Greenhouse is maintained at 20~28 DEG C during hardening, humidity 80~90%, After 1~3 times/day, 3 months of ventilation, the above whole day of height of seedling 25cm opens ceiling, and spring next year band nutrition cup moves on to outdoor or fixed It plants.
3 root-promoting hormone formula of liquid of table
The result shows that: with the quickly dipped root-promoting hormone aqueous solution (indolebutyric acid being configured to by indolebutyric acid and methyl α-naphthyl acetate of cutting Mass ratio with methyl α-naphthyl acetate is 3:1) when, rooting of cuttings rate and next year survival rate are above other root-promoting hormones, than with No. ABT1 Root-inducing powder is that the rooting of cuttings rate of root-promoting hormone improves 2.52%, and next year survival rate increases 26.23%, and nursery stock ratio of height to diameter is also bright It shows to be lower than and uses ABT1 root-inducing powder.
Embodiment 4
The present embodiment is substantially the same manner as Example 3, the difference is that, the quickly dipped root-promoting hormone aqueous solution used of cutting is by Yin The solution of 500mg/kg and 1500mg/kg that diindyl butyric acid and methyl α-naphthyl acetate are configured to, the quality of indolebutyric acid and methyl α-naphthyl acetate in solution Than for 3:1.
4 root-promoting hormone liquid concentration of table
3 cuttage is as a result, the 1000mg/kg~1500mg/kg being configured to by indolebutyric acid and methyl α-naphthyl acetate in conjunction with the embodiments Root-promoting hormone aqueous solution (mass ratio of indolebutyric acid and methyl α-naphthyl acetate be 3:1) effect it is preferable, rooting rate is high, the sturdy hair of root system It reaches, cutting is healthy and strong, and next year survival rate is stablized.To reduce unnecessary waste, thus select indolebutyric acid+methyl α-naphthyl acetate mass ratio for 3:1, concentration are that the root-promoting hormone aqueous solution of 1000mg/kg carries out promoting quickly dipped processing, reach efficient purpose.
The embodiment of the above, only presently preferred embodiments of the present invention, is only used to explain the present invention, not limit The scope of the present invention processed to those of ordinary skill in the art certainly can be according to skill disclosed in this specification Art content, makes other embodiments easily by way of replacing or changing, therefore all in the principle of the present invention and technique item The changes and improvements etc. that part is done, should be included in scope of the present invention patent.

Claims (5)

1. a kind of cuttage breeding method of Cortex Ilicis Rotundae, which is characterized in that specifically includes the following steps:
Step 1: acquisition cutting: choosing health, no disease and pests harm, axillary bud full current year raw semi-lignified branch June~mid-July Item goes terminal bud to be cut into the cutting of long 8~10cm；2~3 axillary buds of reservation in cutting, upper cut truncation, 45 degree of lower cut bevelings, Upper and lower notch is away from nearest axillary bud 0.5cm or more；Retain 1~2, top leaf, every leaf retains 1/3~1/2；
Step 2: the preparation of matrix: according to quality than sawdust: sandy loam: fertile soil: decomposed cow dung is 1:1:4:4 or sawdust: Fertile soil: decomposed cow dung is the ratio mixed raw material of 1:5:4, prepares matrix；
Step 3: matrix, slotting machine disinfection treatment: cuttage the last week carries out 10~20cm to slotting machine and turns over, with 0.5% permanganic acid Potassium carries out disinfection；It mixes proportioned matrix thoroughly sieving, 50% carbendazol wettable powder of 50g is mixed in every cube of matrix, It is covered 3~4 days with film, takes off film；The matrix disinfected is packed into nutrition cup by the 1d before cuttage, debugs temperature and air is opposite Humidity；
Step 4: cuttage: selection of time is in June~mid-July；
Growth-promoting root processing: indolebutyric acid and methyl α-naphthyl acetate are configured to the root-promoting hormone aqueous solution of 1000mg/kg；By the quickly dipped life of cutting Root hormone aqueous solution, dipping the time is 5~8 seconds；
With small wooden, Zha Dong, the depth in hole are 4~5cm, the cutting cuttage that will have been dipped with root-promoting hormone in matrix when cuttage Into matrix, compacting, spray water immediately moisturizing, heat preservation；
Step 5: daily management after inserting:
Humidity: keeping leaf wetness using automatic sprayer, and spray head full diameter is the fog spray-head of 2mm, keeps relative humidity 95% or more；
Increase CO2Gas fertilizer: every morning 9~11 point use CO2Occurrence of equipment increases CO2Gas fertilizer；
Supplementary light: daily 6~8 high-pressure sodium lamp light fillings 2h, 16~19 high-pressure sodium lamp light filling 3h；
Sterilizing: 1 800~1000 times of diluted 50% carbendazol wettable powder or methyl Tobe are alternately sprayed every 15d Saliva fungicide；It is changed to spray 1 time every 20d after rooting of cuttings；
Step 6: hardening transplanting:
It is cultivated 2.5~3 months after taking root, hardening is carried out in greenhouse；Greenhouse is maintained at 20~28 DEG C during hardening, and humidity 80~ 90%, after 1~3 times/day, 3 months of ventilation, the above whole day of height of seedling 25cm opens ceiling, spring next year band nutrition cup move on to it is outdoor or Field planting.
2. a kind of cuttage breeding method of Cortex Ilicis Rotundae according to claim 1, which is characterized in that in the matrix also The compound diammonium hydrogen phosphate fertilizer that mass percent is not more than 2% is added.
3. a kind of cuttage breeding method of Cortex Ilicis Rotundae according to claim 1, which is characterized in that in step 3, the temperature Degree is 25~30 DEG C, and relative air humidity is 80~95%.
4. a kind of cuttage breeding method of Cortex Ilicis Rotundae according to claim 1, which is characterized in that cutting cuttage to matrix In length be cutting overall length 1/2.
5. a kind of cuttage breeding method of Cortex Ilicis Rotundae according to claim 1, which is characterized in that the root-promoting hormone water The mass ratio of indolebutyric acid IBA and methyl α-naphthyl acetate NAA are 3:1 in solution.
CN201811376632.2A 2018-11-19 2018-11-19 A kind of cuttage breeding method of Cortex Ilicis Rotundae Pending CN109220497A (en)

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Citations (4)

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CN106879421A (en) * 2017-03-01 2017-06-23 江苏农林职业技术学院 A kind of purplefruit holly leaf hole tray hardwood cutting and seedling raising method
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Application publication date: 20190118