CN109197575A - The method of Brassica napus recessive gms line and radish selection cross white flower rape - Google Patents
The method of Brassica napus recessive gms line and radish selection cross white flower rape Download PDFInfo
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- CN109197575A CN109197575A CN201811362955.6A CN201811362955A CN109197575A CN 109197575 A CN109197575 A CN 109197575A CN 201811362955 A CN201811362955 A CN 201811362955A CN 109197575 A CN109197575 A CN 109197575A
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- rape
- radish
- white flower
- white
- recessive
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to rape breeding technique fields, disclose the method for a kind of Brassica napus recessive gms line and radish selection cross white flower rape, and choose original parent: cabbage type rape selects stable two based material of recessive cytoblast sterile；Radish selects the white radishes kind for opening white flowers；Distant hybridization: it using pollinating method twice, is carried out in recessive gms line flowering stage；Hybrid seed is harvested, bagging selfing is carried out to the plant for opening white flower fertile in Hybrids F1；In F2 generation, carries out microspore culture, obtains white flower strain material.The present invention effectively reduces the maternal stripping flower bud time, reduces the injury to maternal column cap, exempts influence of the maternal pollen to filial generation；The successful probability of hybrid is improved, the white flower character of radish is introduced into cabbage type rape；The cabbage type rape new material for opening white flower, while the kind of abundant ornamental rape are obtained, ornamental value is improved.
Description
Technical field
The invention belongs to rape breeding technique fields more particularly to a kind of Brassica napus recessive gms line to hybridize with radish
The method of breeding white flower rape.
Background technique
Currently, the prior art commonly used in the trade is such that
Cabbage type rape is the important oil crops of Cruciferae, is the current maximum oil crops of cultivated area in the world
One of, cabbage type rape Brassica napus (AACC, 2n=38) is allotetraploid, is by diploid Chinese cabbage Brassica
Rapa (AA, 2n=20) and diploid wild cabbage Brassica oleracea (CC, 2n=18) hybridization Natural double is formed,
Evolutionary history is shorter, and hereditary basis is narrow.Promote the yield and quality and resistance potentiality of cabbage type rape rape variety
It is badly in need of expanding the hereditary basis of cabbage type rape, it is oil that breeding, which is suitable for agricultural production and the cabbage type rape variety of industrial use,
The target that dish breeder pursues.Rapeseed breeding man always payes attention to the material innovation and breeding of Brassica napus, the competition of present breeding
It will be the integrated innovation competition of new material and new technology.Widen the most important mode of hereditary basis of existing cabbage type rape variety
It is exactly by means such as hybridization, cell fusion, mutagenesis, genetic engineerings.Wherein distant hybridization is rape germplasm innovation and improvement
The Early mature apricot of seed character yellow in mustard type rape, turnip type rape has been transferred to by important means by distant hybridization means
In cabbage type rape variety, the anti-clubroot character of turnip is transferred in cabbage type rape, the sterile character of radish is imported into sweet
Blue type rape.
The method of general routine distant hybridization is to do female parent using by the way of cabage-type rape using normal fertile rape
It carries out, hybridization efficiency is low, is easy to produce pseudostationary, is difficult to receive hybrid seed substantially, even if receiving a small amount of seed, hybrid generation
Breeding difficulty is big.Therefore research selects cross compatibility high, and hybridization success rate is high, high-efficient material and technology, to innovation oil
Dish germ plasm resource plays a significant role.
It produces upper sightseeing tour with the improvement of people ' s living standards to quickly grow, rape sightseeing tour industry development need is more
The rape variety of multi-color, radish (Raphanus sativus L.) Cruciferae Rhaphanus 2 years or annual herb plant, flower
The white flower character of radish, is imported into cabbage type rape variety by white or pink by distant hybridization, is sent out rape tourism business
Exhibition and expansion cabbage type rape gene base resource promote China's rape breeding to have great importance.
In conclusion problem of the existing technology is:
(1) hybridization efficiency is low in the prior art, is easy to produce pseudostationary, is difficult to receive hybrid seed；
(2) amount for receiving seed in the prior art is few, and hybrid generation's breeding difficulty is big；
(3) rape variety of rape sightseeing tour industry development need more multi-color in the prior art.
Solve the difficulty and meaning of above-mentioned technical problem:
In the distant hybridization of rape and radish, true hybrid seed can hardly be obtained by being difficult to succeed, high by selecting
Two based material of recessive cytoblast sterile of compatibility does female parent, can effectively improve the success rate that cabbage type rape hybridizes with radish.Can have
Effect overcomes the case where selecting common cabbage type rape cabage-type rape method to obtain pseudostationary, can be by the success of the shapes such as the white flower of radish
Imported into cabbage type rape variety.The hereditary basis of cabbage type rape can be widened, effectively for educating for cabbage type rape
Kind and the material foundation new using offer.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of Brassica napus recessive gms lines hybridizes with radish
The method of breeding white flower rape,
The invention is realized in this way the side of a kind of Brassica napus recessive gms line and radish selection cross white flower rape
Method:
Step 1, choose original parent: cabbage type rape selects stable two based material GQAB-3 of recessive cytoblast sterile；Radish
Select the white radishes kind for opening white flower；
Distant hybridization: step 2 using pollinating method twice, is carried out in recessive gms line flowering stage, is pollinated for the first time
It is once pollinated again with the pollen of male parent again within second day afterwards；
Step 3, maturity period harvest hybrid seed, the method that Hybrids F1 uses culturing and transplanting seedlings, to opening white flower in Hybrids F1
Fertile plant carries out bagging selfing, obtains F2；
Step 4, the F2 seville orange flower powder that the elite seedling of white flower is opened in selection carry out microspore culture, obtain stable white flower strain
Based material.
Further, in step 1, cabbage type rape selects yielding ability good, and oil content is high, cross compatibility is high, stablizes
Two based material of recessive cytoblast sterile cabbage type rape；
Further, in step 2, using pollinating method twice, after pollinating for the first time second day again with the pollen of male parent again into
The primary pollination of row.
In conclusion advantages of the present invention and good effect are as follows:
A kind of method of Brassica napus recessive gms line and radish selection cross white flower rape provided by the invention uses
Sterile plant in two system of recessive cytoblast sterile with high-affinity makees female parent material and carries out cabbage type rape distant hybridization, can be effective
The maternal stripping flower bud time is reduced, while reducing stripping flower bud and maternal column cap is damaged.Exempt maternal pollen to filial generation simultaneously
It influences.Make female parent to rape than in the prior art to be difficult to receive seed or substantially can not receive to hybridize with radish progress distant hybridization
Seed has greatly etc. (2006) to do female parent with 3 cabbage type rapes recklessly and hybridizes 1206 flowers of cohybridization with radish, obtains 37
Hybridize silique, wherein in vitro culture obtains 25 siliques, and field control obtains 12 siliques, and field compares 12 angles obtained
Fruit, which is confiscated, receives a seed.Make female parent in the present invention with high affine cabbage type rape recessive cytoblast sterile to hybridize with white flower radish,
The successful probability of hybrid can be effectively improved, the merit of radish is introduced into cabbage type rape.There is many in radish
Advantageous character, for example, cytoplasmic male sterility and restoring gene and selfing incompatibility gene, excellent degeneration-resistant and disease-resistant (anti-
Soft rot, black rot, clubroot) insect resistace (anti-root-knot nematode) etc..In plant genetics and breeding, distant hybridization can be by radish
In excellent genes import cabbage type rape, to widen the hereditary basis of cabbage type rape, improve cabbage type rape, pass through this
Hybridization obtains out the cabbage type rape new material of white flower, while the kind of abundant ornamental rape, improves ornamental value.
Detailed description of the invention
Fig. 1 is the breeding side that Brassica napus recessive gms line provided in an embodiment of the present invention hybridizes white flower rape with radish
Method flow chart.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The present invention will be described in further detail with reference to the accompanying drawing；
As shown in Figure 1, Brassica napus recessive gms line provided in an embodiment of the present invention hybridizes the choosing of white flower rape with radish
Educate method:
S101: choose original parent: cabbage type rape selects stable two based material GQAB-3 of recessive cytoblast sterile；Radish choosing
With the white radishes kind for opening white flower；
S102: it distant hybridization: using pollinating method twice, is carried out in recessive gms line flowering stage, after pollinating for the first time
It is once pollinated again with the pollen of male parent again within second day；
S103: the maturity period harvests hybrid seed, and the method that Hybrids F1 uses culturing and transplanting seedlings can to open white flower in Hybrids F1
It educates plant and carries out bagging selfing, obtain F2；
S104: the F2 seville orange flower powder that the elite seedling of white flower is opened in selection carries out microspore culture, obtains stable white flower strain
Material.
In step S101, cabbage type rape provided in an embodiment of the present invention, selection cross compatibility is high, yielding ability is good, contains
High stable two based material of cabbage type rape recessive cytoblast sterile of oil mass；
In step S102, the embodiment of the present invention is provided using pollinating method twice, uses father again within second day after pollinating for the first time
This pollen is once pollinated again.
Application principle of the invention is further described combined with specific embodiments below.
Wild cabbage type recessive cytoblast sterile rape GQAB-3 has many advantages, such as that seedling stage rising trend is prosperous, oil content is high, compatibility is strong, 2012
March in year is pollinated with radish white flower to the sterile plant in GQAB-3, and 50 flowers of pollinating, in May, 2012 harvests 8 siliques, obtains 15
15 seed kinds of acquisition in Nutrition Soil, are emerged 12 plants, when seedling grows to 2-3 piece true leaf, are selected by seed, in September, 2013
Cloudy day is transplanted to experimental plot, 7 days holding ground moistenings after transplanting, it is ensured that and transplanting seedling survives, spring rape florescence in 2014, and 12
The whole strains of strain are bloomed, wherein having one plant to open white flower is sterile plant, having one plant to open white flower normally has powder, remaining 10 plants are normal
There is powder to open chrysanthemum plant, by open white flower have powder plant bagging selfing, 2015 spring year selected in F2 high yield white flower plant into
Row microspores culture obtains stable white flower cabbage type rape strain in 2016.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (3)
1. a kind of method of Brassica napus recessive gms line and radish selection cross white flower rape, which is characterized in that the wild cabbage
Rapeseed plant recessive line with genic sterile hybridizes the selection of white flower rape with radish:
Step 1, choose original parent: cabbage type rape selects stable two based material GQAB-3 of recessive cytoblast sterile；Radish is selected
Open the white radishes kind of white flower；
Step 2, distant hybridization: using pollinating method twice, carrying out in recessive gms line flowering stage, the after pollinating for the first time
It is once pollinated again with the pollen of male parent again within two days；
Step 3, maturity period harvest hybrid seed, the method that Hybrids F1 uses culturing and transplanting seedlings, to opening the fertile of white flower in Hybrids F1
Plant carries out bagging selfing, obtains F2；
Step 4, the F2 seville orange flower powder that the elite seedling of white flower is opened in selection carry out microspore culture, obtain stable white flower strain material
Material.
2. Brassica napus recessive gms line as described in claim 1 hybridizes the selection of white flower rape, feature with radish
It is, in the step 1, cabbage type rape selects yielding ability good, the recessiveness that oil content is high, cross compatibility is high, stable
The cabbage type rape of gms two-line system material.
3. Brassica napus recessive gms line as described in claim 1 hybridizes the selection of white flower rape, feature with radish
It is, in the step 2, using pollinating method twice, is carried out again with the pollen of male parent again within second day after pollinating for the first time primary
Pollination.
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| CN201811362955.6A CN109197575A (en) | 2018-11-16 | 2018-11-16 | The method of Brassica napus recessive gms line and radish selection cross white flower rape |
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| CN201811362955.6A CN109197575A (en) | 2018-11-16 | 2018-11-16 | The method of Brassica napus recessive gms line and radish selection cross white flower rape |
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| JP2008504801A (en) * | 2004-07-05 | 2008-02-21 | アンスティテュ ナシオナル ドゥ ラ ルシェルシュ アグロノミック | A method for producing a double low content recovery line of Brassica napus with high agricultural value |
| CN102475057A (en) * | 2010-11-23 | 2012-05-30 | 华中农业大学 | Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line |
| CN102696474A (en) * | 2012-05-15 | 2012-10-03 | 西北农林科技大学 | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish |
| CN107347632A (en) * | 2017-08-24 | 2017-11-17 | 湖南省作物研究所 | A kind of selection of cabbage type rape radish cytoplasmic sterility restorer and the application in cabbage type rape breeding |
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| CN102475057A (en) * | 2010-11-23 | 2012-05-30 | 华中农业大学 | Selection breeding method for a Brassica napus-turnip cabbage cytoplasmic male sterility line |
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