CN109182314A - A kind of extracting method of honey enzyme activity substance - Google Patents

A kind of extracting method of honey enzyme activity substance Download PDF

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Publication number
CN109182314A
CN109182314A CN201810756792.3A CN201810756792A CN109182314A CN 109182314 A CN109182314 A CN 109182314A CN 201810756792 A CN201810756792 A CN 201810756792A CN 109182314 A CN109182314 A CN 109182314A
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China
Prior art keywords
honey
enzyme system
physiological activator
extracting method
former
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Pending
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CN201810756792.3A
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Chinese (zh)
Inventor
张治刚
刘胜利
高雪琴
刘庆芬
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Zhengzhou Cool Biotechnology Co Ltd
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Zhengzhou Cool Biotechnology Co Ltd
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Priority to CN201810756792.3A priority Critical patent/CN109182314A/en
Publication of CN109182314A publication Critical patent/CN109182314A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004—Glucose oxidase (1.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01006—Catalase (1.11.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of extracting methods of honey enzyme system physiological activator, belong to technical field of enzyme engineering.By to former honey carry out pure water dilution, heat temperature raising, coarse filtration, refined filtration, ultrafiltration and etc. purification processes, honey enzyme system physiological activator is made, to solve the problems, such as that physiological activator concentration is low in former honey, for diabetic, the patients such as uric acid height, gout, fat and some hyperlipemic patients inedibility honey due to the high sugar content of honey, and it needs the nutrition of honey enzyme system physiological activator to provide health and is helped with rehabilitation, while new healthy food source being also provided for general population.

Description

A kind of extracting method of honey enzyme activity substance
Technical field
The techniques such as coarse filtration, refined filtration, ultrafiltration are carried out using honey the present invention relates to a kind of, make honey enzyme system physiological activity object The extracting method category technical field of enzyme engineering of matter.
Background technique
Honey it is complex in composition, it is full of nutrition, have several physiological active substances, be the mankind tradition and ancient natural battalion Support health care product.In honey glucose content be about 35%, fructose 40%, sucrose 2%, dextrin 1%, be all golf calorific value substance.In addition, The several physiological active substances such as nitrogenous compound also rich in and honey enzyme system.
Honey enzyme system specifically includes that invertase, amylase, glucose oxidase, catalase, in addition, there are also restore Enzyme, lipase, albuminoid enzyme etc., these enzymes have highly important effect in metabolism, are that honey unique medical health care is made Main matter.But it due to the high sugar content of honey, limits part and the nutrition of honey enzyme system physiological activator is needed to protect Crowd strong and that honey cannot be taken.These crowds mainly have: 1. diabetic, because honey contains a large amount of monosaccharide, very It is easily accessible blood, the state of an illness can be aggravated.2. the symptoms such as uric acid is high, gout cannot frequently eat honey, because can fall to cause uric acid liter Height aggravates the state of an illness of gout.3. fat and some hyperlipemic patients inedibility honey.
Very good solution of the present invention problem above, provides honey enzyme system physiological activity product for these crowds, preferably Promote the health of these three types of crowds, meanwhile, new healthy food source is also provided for general population.
Summary of the invention
The object of the present invention is to provide a kind of extracting methods of honey enzyme system physiological activator.
A kind of the object of the present invention is achieved like this: extracting method of honey enzyme system physiological activator.
Pretreatment: 1~1.5 times of pure water is added in former honey pot and dilutes and stirs evenly for former honey, is passed through steam liter Temperature is to 38~42 DEG C.
Coarse filtration: diatomite flame filter press is squeezed into pump by pretreated former honey and carries out coarse filtration, diatomite model YC10。
Refined filtration: refined filtration liquid enters 10um sintered pipes device and carries out refined filtration.
Ultrafiltration: the filtrate pump by refined filtration enters ultrafiltration apparatus (pressure is controlled in 0.3~0.6MPa), ultrafiltration membrane model UFIOB9L, material are polysulfones (PS), and molecular cut off 6000Da, concentrate circulating is into green molasses tank.
Collect: by coarse filtration, refined filtration, the circular treatment of ultrafiltration, finally obtained concentrate is that honey enzyme system physiology is living Property substance, is collected into finished pot from green molasses tank.
Detailed description of the invention
Fig. 1, honey enzyme system physiological activator extraction process schematic diagram
Fig. 2, honey enzyme system physiological activator extraction process flow chart
Specific embodiment
Embodiment one
Take 100kg original honey to be put into green molasses tank, be added 100kg pure water dilute and stirs evenly, be passed through steam to dilute simultaneously The honey stirred evenly is heated to 42 DEG C, through pump squeeze into the diatomite flame filter press having had been coated with (diatomite model are as follows: YC10 coarse filtration) is carried out, coarse filtration liquid is sent into 10um sintered pipes device refined filtration, and refined filtration liquid keeps 0.6MPa pressure through pump, is sent into ultrafiltration Device (ultrafiltration membrane model are as follows: UFIOB9L, material are polysulfones (PS), molecular cut off 6000Da), the concentrate circulating after ultrafiltration Into green molasses tank, the circulation by system is concentrated, and final concentrate is honey enzyme system physiological activator.
Embodiment two
Take 100kg original honey to be put into green molasses tank, be added 150kg pure water dilute and stirs evenly, be passed through steam to dilute simultaneously The honey stirred evenly is heated to 38 DEG C, through pump squeeze into the diatomite flame filter press having had been coated with (diatomite model are as follows: YC10 coarse filtration) is carried out, coarse filtration liquid is sent into 10um sintered pipes device refined filtration, and refined filtration liquid keeps 0.3MPa pressure through pump, is sent into ultrafiltration Device (ultrafiltration membrane model are as follows: UFIOB9L, material are polysulfones (PS), molecular cut off 6000Da), the concentrate circulating after ultrafiltration Into green molasses tank, the circulation by system is concentrated, and final concentrate is honey enzyme system physiological activator.
It above are only the specific embodiment of the present invention, but the design concept of the present invention is not limited to this, all benefits It is made a non-material change to the present invention, should all be belonged to behavior that violates the scope of protection of the present invention with this design.

Claims (4)

1. a kind of extracting method of honey enzyme system physiological activator, it is characterised in that following steps:
(1) pre-process: 1~1.5 times of pure water is added in former honey pot and dilutes and stirs evenly for former honey, is passed through steam heating To 38~42 DEG C;
(2) coarse filtration: diatomite flame filter press is squeezed into pump by pretreated former honey and carries out coarse filtration, diatomite model YC10;
(3) refined filtration: refined filtration liquid enters 10um sintered pipes device and carries out refined filtration;
(4) ultrafiltration: the filtrate pump by refined filtration enters ultrafiltration apparatus (pressure is controlled in 0.3~0.6MPa), ultrafiltration membrane model are as follows: UFIOB9L, material are polysulfones (PS), and molecular cut off 6000Da, concentrate circulating is into green molasses tank.
2. a kind of extracting method of honey enzyme system physiological activator according to claim 1, it is characterised in that: in former bee 1~1.5 times of pure water is added in honey jar to dilute and stir evenly.
3. a kind of extracting method of honey enzyme system physiological activator according to claim 1, it is characterised in that: be passed through steaming Vapour is warming up to 38~42 DEG C.
4. a kind of extracting method of honey enzyme system physiological activator according to claim 1, it is characterised in that: by essence The filtrate pump of filter enters ultrafiltration apparatus (pressure is controlled in 0.3~0.6MPa).
CN201810756792.3A 2018-07-11 2018-07-11 A kind of extracting method of honey enzyme activity substance Pending CN109182314A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810756792.3A CN109182314A (en) 2018-07-11 2018-07-11 A kind of extracting method of honey enzyme activity substance

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810756792.3A CN109182314A (en) 2018-07-11 2018-07-11 A kind of extracting method of honey enzyme activity substance

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CN109182314A true CN109182314A (en) 2019-01-11

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110609033A (en) * 2019-10-31 2019-12-24 中国农业科学院蜜蜂研究所 Method for detecting enzyme value of sucrose invertase in honey
CN111434243A (en) * 2019-01-23 2020-07-21 重庆悬泉蜂业有限公司 Deep processing extraction method for extracting active substances from honey

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111434243A (en) * 2019-01-23 2020-07-21 重庆悬泉蜂业有限公司 Deep processing extraction method for extracting active substances from honey
CN110609033A (en) * 2019-10-31 2019-12-24 中国农业科学院蜜蜂研究所 Method for detecting enzyme value of sucrose invertase in honey

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Application publication date: 20190111