CN103110098A - Method for preparing effective component of mottle clam health-care product with hypoglycemic effect - Google Patents
Method for preparing effective component of mottle clam health-care product with hypoglycemic effect Download PDFInfo
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- CN103110098A CN103110098A CN2013100116681A CN201310011668A CN103110098A CN 103110098 A CN103110098 A CN 103110098A CN 2013100116681 A CN2013100116681 A CN 2013100116681A CN 201310011668 A CN201310011668 A CN 201310011668A CN 103110098 A CN103110098 A CN 103110098A
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Abstract
The invention discloses a method for preparing the effective component of a mottle clam health-care product with a hypoglycemic effect. The AGD (Alpha-Glucosaccharase) component contained in a mottle clam is obtained by adopting an enzymolysis technology on the mottle clam, and an AGD inhibitor can enable the postprandial blood sugar peak value of people to be gradually lowered and reduced in fluctuation so that glycosylated hemoglobin contained in blood plasma is reduced. The health-care product processed by utilizing the effective component has good activity inhibition effect on the alpha-glucosaccharase without side effect on a human body and can provide the amino acid needed by the human body, thereby becoming an auxiliary curing health-care product used for a diabetes patient. The method disclosed by the invention has the advantages of easily available raw materials and low price of raw materials, simple processing method, small investment, high additional value and very wide application prospect and can be widely used for producing the health-care product; and in addition, the method disclosed by the invention can stimulate the consumption domestic demand of the mottle clam, thereby accelerating the development of a mottle clam culture industry.
Description
Technical field
The present invention relates to a kind of preparation method of health products active ingredient, particularly a kind of preparation method with variegated clam health products active ingredient of hypoglycemic activity.
Background technology
Variegated clam meat matter is delicate, and delicious flavour is nutritious.In recent years, China's variegated clam cultivation scale constantly enlarges, output is with sharp increase, but the processing mode of present variegated clam is comparatively single, be mainly low value-added fresh goods and freeze and boil converted products, directly affected breeding enterprise economic benefit and farmers' income, hindered clam class cultivation scale and further enlarged.Therefore variegated clam is carried out intensive processing, the development high value-added product is undoubtedly development, grows and optimizes China's shellfish industry, promotes aquaculture and processing industry to work in coordination with increment, cultivates the primary approach of our province marine economy new growth point.
Diabetes are lifelong participation chronic diseases of a kind of common serious harm health, and main manifestations is chronic hyperglycemia and with carbohydrate, fat and protein metabolism disturbance.For patients with NIDDM, control postprandial plasma glucose level and can effectively slow down diabetes process and prevention diabetic complication.Alpha-glucosidase (AGD) is present on the microvillus of intestinal brush border cell, the hydrolysis of the carbohydrate of complexity can be become monose and be absorbed and enter blood.The AGD inhibitor can make people's glycemic peaks after the meal gradually reduce, and fluctuation reduces, and the glycosylated hemoglobin in blood plasma is reduced, thereby the AGD inhibitor is also one of the 5 main non-insulin type of class medicines.The AGD inhibitor of natural origin is a lot, but does not still obtain at present the report of AGD inhibitor by the variegated clam of hydrolysis.
Summary of the invention
The present invention is in order to solve the existing above-mentioned deficiency of prior art, propose a kind ofly can obtain alpha-glucosidase in variegated clam (AGD) composition, and it is prepared into the method for health products core active ingredient, the health products that adopt this active ingredient to prepare have significant hypoglycemic activity, are a kind of very suitable health products for patients with NIDDM.
Technical solution of the present invention is: a kind of preparation method with variegated clam health products active ingredient of hypoglycemic activity, and it is characterized in that: described method is carried out according to following steps:
A, get variegated clam meat, smash to pieces through tissue mashing machine, obtain the clam meat meat gruel,
B, being 1:5-30 according to mass ratio with clam meat meat gruel and deionized water, ratio is evenly puddled, and obtains clam meat homogenate,
C, to add enzyme activity unit in the clam meat homogenate be the pepsin of 1:3000, the pepsic quality that adds is the 1-2.5% of variegated clam meat quality, regulates the pH to 2-3 of mixed liquor, is under the condition of 30-45 ℃ enzymolysis 1-5 hour in temperature, obtain variegated clam meat enzymolysis liquid
D, variegated clam meat enzymolysis liquid is heated to 90-100 ℃, kept 10-15 minute, regulate pH value to 7, the acquisition clam meat enzyme liquid that goes out,
E, with go out enzyme liquid under the condition of 3000-8000 rev/min centrifugal treating 10-30 minute of clam meat, get supernatant,
F, supernatant is carried out drying process, obtain variegated clam health products active ingredient.
The present invention compares with prior art, has following advantage:
The method disclosed in the present can the guidance technology personnel be prepared a kind of variegated clam health products active ingredient with hypoglycemic activity.It adopts zymolysis technique to variegated clam, obtains alpha-glucosidase (AGD) composition in variegated clam, and the AGD inhibitor can make the minimizing that reduces gradually, fluctuates of people's glycemic peaks after the meal, makes the glycosylated hemoglobin reduction in blood plasma.The health products that utilize this active ingredient processing to make have alpha-glucosidase and suppress preferably active, and human body is had no side effect, and the amino acid of needed by human body can be provided simultaneously, therefore can become diabetes patient's auxiliary curing health products.Raw materials wide material sources of the present invention, low price, processing method is simple, small investment, added value is high, can be widely used in the production health product, and application prospect is very wide.It can also pull the consumption domestic demand of variegated clam simultaneously, and then accelerates the development of variegated clam aquaculture industry.
The specific embodiment
The below will illustrate the specific embodiment of the present invention.
Embodiment one
Get fresh and alive variegated clam, get meat after cleaning, smash to pieces through tissue mashing machine and obtain variegated clam meat paste, add in the meat gruel to the deionized water of 20 times of quality and mix, obtaining clam meat homogenate, the pepsin (enzyme activity unit is 1:3000) of the clam meat quality 2% that adds Noise in the clam meat homogenate, and the pH value of mixed liquor is adjusted to 2.0, be that under the condition of 37 ℃, enzymolysis 2 hours obtains variegated clam meat enzymolysis liquid in temperature; Above-mentioned variegated clam meat enzymolysis liquid is heated to 95 ℃, kept 10 minutes, regulate pH to 7.0 with NaOH, obtain the clam meat enzyme liquid that goes out; With the clam meat enzyme liquid centrifugal treating 10 minutes under the condition of 5000 rev/mins of going out, then get supernatant; Supernatant is carried out freeze drying process, obtain solid nutrient and healthcare products active ingredient.
Detect through experiment in vitro, this kind active ingredient can reach more than 50% the inhibiting rate of alpha-glucosidase.
Embodiment two
Get fresh and alive variegated clam, get meat after cleaning, smash to pieces through tissue mashing machine and obtain variegated clam meat paste, add in the meat gruel to the deionized water of 15 times of quality and mix, obtaining clam meat homogenate, the pepsin (enzyme activity unit is 1:3000) of the clam meat quality 1.5% that adds Noise in the clam meat homogenate, and the pH value of mixed liquor is adjusted to 2.0, be that under the condition of 37 ℃, enzymolysis 2.5 hours obtains variegated clam meat enzymolysis liquid in temperature; Above-mentioned variegated clam meat enzymolysis liquid is heated to 100 ℃, kept 10 minutes, regulate pH to 7.0 with NaOH, obtain the clam meat enzyme liquid that goes out; With the clam meat enzyme liquid centrifugal treating 15 minutes under the condition of 4000 rev/mins of going out, then get supernatant; Supernatant is carried out spray drying treatment, obtain solid nutrient and healthcare products active ingredient.
Detect through experiment in vitro, this kind active ingredient can reach more than 50% the inhibiting rate of alpha-glucosidase.
The supernatant that the present invention is obtained can adopt the methods such as reduced pressure concentration, freeze drying or spray-drying to process.
The active ingredient that makes take the method for the invention is added one or more auxiliary material and is made health products as the basis.Described auxiliary material is additive or filler commonly used in food, and as xylitol, starch, dextrin etc., the content of described auxiliary material does not have strict restriction, can be different according to technique and needs.Can granulation or make capsule or make other formulation according to conventional method.
Claims (1)
1. preparation method with variegated clam health products active ingredient of hypoglycemic activity, it is characterized in that: described method is carried out according to following steps:
A, get variegated clam meat, smash to pieces through tissue mashing machine, obtain the clam meat meat gruel,
B, being 1:5-30 according to mass ratio with clam meat meat gruel and deionized water, ratio is evenly puddled, and obtains clam meat homogenate,
C, to add enzyme activity unit in the clam meat homogenate be the pepsin of 1:3000, the pepsic quality that adds is the 1-2.5% of variegated clam meat quality, regulates the pH to 2-3 of mixed liquor, is under the condition of 30-45 ℃ enzymolysis 1-5 hour in temperature, obtain variegated clam meat enzymolysis liquid
D, variegated clam meat enzymolysis liquid is heated to 90-100 ℃, kept 10-15 minute, regulate pH value to 7, the acquisition clam meat enzyme liquid that goes out,
E, with go out enzyme liquid under the condition of 3000-8000 rev/min centrifugal treating 10-30 minute of clam meat, get supernatant,
F, supernatant is carried out drying process, obtain variegated clam health products active ingredient.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100116681A CN103110098A (en) | 2013-01-14 | 2013-01-14 | Method for preparing effective component of mottle clam health-care product with hypoglycemic effect |
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| CN2013100116681A CN103110098A (en) | 2013-01-14 | 2013-01-14 | Method for preparing effective component of mottle clam health-care product with hypoglycemic effect |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319012A (en) * | 2016-08-19 | 2017-01-11 | 江南大学 | Method for preparing Alpha-glucosidase inhibitor from Meretrix protein |
| CN106905417A (en) * | 2017-04-24 | 2017-06-30 | 南京中医药大学 | Peptide for inhibiting of a kind of dipeptidyl peptidase 4 and preparation method thereof is applied with it |
| CN106905416A (en) * | 2017-04-24 | 2017-06-30 | 南京中医药大学 | With active peptide for suppressing dipeptidyl peptidase 4 and preparation method and application |
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| CN1552251A (en) * | 2003-06-03 | 2004-12-08 | 周唯贤 | Hypolycemic and antilipemic clam food with immunity regulation |
| CN101731615A (en) * | 2009-12-30 | 2010-06-16 | 大连水产学院 | Preparation method of clam nutrition health care condiment |
| CN101803788A (en) * | 2010-03-19 | 2010-08-18 | 中国水产科学研究院南海水产研究所 | Keep-alive method of Ruditapes philippinarum |
| KR20120049046A (en) * | 2010-11-08 | 2012-05-16 | 건국대학교 산학협력단 | Antiinflammatory composition comprising enzymatic hydrolysates of ruditapes philippinarum |
| KR20120049045A (en) * | 2010-11-08 | 2012-05-16 | 건국대학교 산학협력단 | Antioxidant composition comprising enzymatic hydrolysates of ruditapes philippinarum |
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2013
- 2013-01-14 CN CN2013100116681A patent/CN103110098A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1552251A (en) * | 2003-06-03 | 2004-12-08 | 周唯贤 | Hypolycemic and antilipemic clam food with immunity regulation |
| CN101731615A (en) * | 2009-12-30 | 2010-06-16 | 大连水产学院 | Preparation method of clam nutrition health care condiment |
| CN101803788A (en) * | 2010-03-19 | 2010-08-18 | 中国水产科学研究院南海水产研究所 | Keep-alive method of Ruditapes philippinarum |
| KR20120049046A (en) * | 2010-11-08 | 2012-05-16 | 건국대학교 산학협력단 | Antiinflammatory composition comprising enzymatic hydrolysates of ruditapes philippinarum |
| KR20120049045A (en) * | 2010-11-08 | 2012-05-16 | 건국대학교 산학협력단 | Antioxidant composition comprising enzymatic hydrolysates of ruditapes philippinarum |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106319012A (en) * | 2016-08-19 | 2017-01-11 | 江南大学 | Method for preparing Alpha-glucosidase inhibitor from Meretrix protein |
| CN106905417A (en) * | 2017-04-24 | 2017-06-30 | 南京中医药大学 | Peptide for inhibiting of a kind of dipeptidyl peptidase 4 and preparation method thereof is applied with it |
| CN106905416A (en) * | 2017-04-24 | 2017-06-30 | 南京中医药大学 | With active peptide for suppressing dipeptidyl peptidase 4 and preparation method and application |
| CN106905417B (en) * | 2017-04-24 | 2020-11-24 | 南京中医药大学 | Dipeptidyl peptidase-4 inhibitory peptide, and preparation method and application thereof |
| CN106905416B (en) * | 2017-04-24 | 2020-11-24 | 南京中医药大学 | Active peptide capable of inhibiting dipeptidyl peptidase-4 and preparation method and application thereof |
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Application publication date: 20130522 |