KR20070116201A - Processing excellent taste and flavor containing vinegar using sea tangle(laminaria japonica) - Google Patents

Processing excellent taste and flavor containing vinegar using sea tangle(laminaria japonica) Download PDF

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KR20070116201A
KR20070116201A KR1020070045819A KR20070045819A KR20070116201A KR 20070116201 A KR20070116201 A KR 20070116201A KR 1020070045819 A KR1020070045819 A KR 1020070045819A KR 20070045819 A KR20070045819 A KR 20070045819A KR 20070116201 A KR20070116201 A KR 20070116201A
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kelp
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    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00Microorganisms ; Processes using microorganisms
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    • C12R2001/02Acetobacter

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Abstract

A method for preparing sea tangle vinegar with good taste is provided to develop the vinegar from sea tangle having excellent nutrition by including physiologically active materials, fucoxanthin, chlorophyll, fucoidan, alginic acid, mannitol, laminarin, various minerals and vitamins and functionality. A method for preparing sea tangle vinegar comprises the steps of: (a) adding apple juice to clear strained rice wine or distilled wine made from sea tangle with mixing to prepare a culture medium; and (b) passing the culture medium through a column filled up with porous ceramics or basalt beads wherein acetic acid bacteria is fixed to be proceeded with the acetic acid fermentation.

Description

맛이 아주 좋은 다시마 식초의 제조방법 { Processing Excellent Taste and Flavor containing Vinegar using sea tangle(Laminaria japonica)}How to make a tasty seaweed vinegar {Processing Excellent Taste and Flavor containing Vinegar using sea tangle (Laminaria japonica)}

다시마 식초 제조에 관한 등록된 특허는 찾아 볼 수 없으나 공개되고 있는 것이 다음과 같이 우리나라 2건, 일본 1건이 특허문헌조사결과 발견되는데 이 것들 모두가 초산발효를 위하여 첨가하는 주정자체를 특별하게 고려하지 않고 또 초산발효도 2번에 걸처 실시되거나, 정치상태로 서서히 진행 시키므로 4주간 정도 소요되고 있다. 특히 다시마의 감칠맛과 생리 활성 물질을 가진 다시마액을 기반으로 하는 다시마 식초 제조에는 상당한 거리가 있는 다시마 액 추출, 제조공정으로 가압/열수, 산/열수, 혹은 알카리/온수 처리등으로 획득되는 다시마 추추액은 그 감칠맛이 상당히 파괴되고 또 알긴산이 많이 추출되어 점도가 높은 액이 되므로 고려 대상이라 사료 되고, 또 일본에서 적용한 냉수로 장시간(20-30시간)추출한 다시마액 역시 장시간 추출에 따른 액의 점도와 맛이 고려 대상이라 사료된다. 이하 그 공개 내용을 간단히 요약 기술하면 다음과 같다.There are no registered patents related to the production of kelp vinegar, but it is disclosed that two cases of Korea and one case of Japan are found as follows. All of these do not take special consideration of the alcohol itself added for acetic acid fermentation. In addition, acetic acid fermentation is also carried out twice, or it takes about four weeks because it gradually progresses to a stationary state. In particular, kelp vinegar based on kelp flavour with kelp taste and physiologically active substance is extracted from kelp liquor which has a considerable distance, and it is obtained by pressurized / hot water, acid / hot water, or alkaline / hot water treatment. The extract is considered to be considerably deteriorated and its rich flavor is extracted and the alginic acid is extracted to make it a highly viscous liquid. Also, the kelp liquid extracted for a long time (20-30 hours) with cold water applied in Japan also has a viscosity And taste are considered to be considered. The following is a brief summary of the disclosure.

공개특허공보 특2002-0076364(2002.10.11) 다시마 분말을 열수로 1시간 추출 여과하여 Brix 2.5로 조절 하고 에칠알코홀 5%, 초산4%, 포도당 3%, 혹은 5% 그외 영양재 2종을 첨가하여 섭씨30도에서 1주 발효 중초를 만들고 이를 다시마추추액과 50 대 50으로 혼합하여 2차 초산 발효를 섭씨30도에서 20일간 실시 다시마 식초를 제조하는 방법.The kelp powder was extracted and filtered by hot water for 1 hour, adjusted to Brix 2.5, and 5% ethyl alcohol, 4% acetate, 3% glucose, or 5% other nutrients were added. 1 week fermented medium vinegar at 30 degrees Celsius to mix it with 50 to 50 kelp extract and the second acetic acid fermentation method carried out for 20 days at 30 degrees Celsius.

공개 특허 공보 10-2005-0065964(2005.06.30)다시마유래의 올리고당과 식이섬유를 함유한 다시마 식초의 제조방법은 다시마가수분해물을 이용한 다시마식초의 제조로 그 방법은 상기한 방법과 대동소이하다. 즉 가압/열수, 강산/열수, 혹은 강알카리/온수로 추출한 다시마액에 당류 5-20%, 주정5-12%, 초산 2-4%, 및 영양재 2-3종을 첨가한 배지를 사용하여 중초발효를 5일간 실시하고 이를 다시마 추출액과 50:50으로 혼합하여 10일간 초산발효를 실시하여 획득하는 다시마식초.The production method of kelp vinegar containing oligosaccharide and dietary fiber derived from Dashima is similar to the method described above, which is prepared from kelp hydrolyzate. That is, a medium containing 5-20% of sugar, 5-12% of alcohol, 2-4% of acetic acid, and 2-3 kinds of nutrients was added to kelp liquid extracted with pressurized / hot water, strong acid / hot water, or strong alkali / hot water. The fermented seaweed is fermented for 5 days and mixed with kelp extract at 50:50 to obtain acetic acid fermentation for 10 days.

일본 공개특허공보 특개2003-153683(2003.05.27) 昆布酢의 製造方法에 따르면 다시마 추출액, 주정, 및 사과즙을 배지로하여 초산균을 접종 통기 정치배양을 4주간 실시하여 초산 4.2%, 맥아즙을 첨가하여 초산 5%의 다시마 식초 제조방법. 일본의 다시마 추출액은 저온에서 20-30시간 실시하고 있어 미생물의 오염은 상당하게 제한 될지 모르나 장시간의 추출로 알긴산 등의 추출이 예상되어 깔끔하고 감칠맛이 나는 다시마 식초에는 거리가 있는것으로 사료된다.According to the method of Japanese Patent Application Laid-Open No. 2003-153683 (2003.05.27), the aerobic inoculation of acetic acid bacterium was carried out using kelp extract, spirit, and apple juice as a medium for 4 weeks to add 4.2% acetic acid and wort. Seaweed vinegar production method of 5% acetic acid. The kelp extract in Japan is carried out at low temperature for 20-30 hours, so the contamination of microorganisms may be considerably limited, but the extraction of alginic acid is expected with long time extraction.

이상 다시마 식초의 제조방법을 검토하면 첫째 다시마 추출액 제조에 개선의 여지가 있다, 즉 다시마에 함유된 맛성분을 단시간내 추출 농축하여 요구되는 맛과 농도를 달성하고 맛에 장애가 되는 폴리머의 추출을 제한하는 방법이 필요하며, 한편으로는 일반 주정을 사용(6-9%) 초산발효 하는것으로는 다시마 식초로서의 향과 맛이 다시마를 주정발효하여 양조한 청주 혹은 증류주를 사용하는것에 따르지 못하 는 점 등을 고려 해야 할것이며, 다시마 추추액에 어느정도 익숙한(길 드려진) 초산균을 계속 사용할 수 있는 방법을 고려한다면 개선의 여지가 충분이 있다고 사료된다.If the above method of preparing kelp vinegar is examined, there is room for improvement in the preparation of kelp extract, that is, extracting and concentrating the flavor components contained in the kelp in a short time to achieve the required taste and concentration and limit the extraction of polymers that interfere with taste. On the other hand, acetic acid fermentation using ordinary spirits (6-9%) does not depend on the use of sake or brewed sake brewed by brewing kelp or distilled liquor. Consideration should be given to the use of acetic acid bacteria, which are somewhat familiar with kelp weights, and there is ample room for improvement.

아주 맛이 좋은 다시마 식초를 경재적으로 량산(제조)하는 공법을 개발하는 것으로 다시마의 감칠맛과 영양성분을 어느 정도 함유하면서도 맛을 내는데 바람직하지 않는 알긴산 등이 포함되는것을 제한하기 위하여 다시마 액 추출시간을 짧은 시간으로 단축(바람직하게는 1시간)하고 한편 맛과 농도를 달성하기위하여 이를 저온(섭씨 50도)에서 진공 농축하여 맛과 향 및 비타민 등이 살아있는 다시마 추출액을 준비하고, 다시마를 주정발효에 사용하여 양조한 다시마 술[이 분활 출원특허와 같이 분활출원되는 방법으로 제조되는 다시마 술(청주 혹은 증류주)], 그리고 초산발효에 촉매와 역활을 하는 사과즙을 잘 혼합하여 준비한 배지를 현무암에 고정화화 초산균담체를 사용 연속적으로 식초를 양조하여 경재적이면서도 아주 맛이 좋은 다시마 식초를 제조하는 것이다.Development of a method to produce a very good taste of kelp vinegar economically, to limit the inclusion of alginic acid, which is undesirable to taste while containing some of the rich flavor and nutritional ingredients of kelp In order to shorten (preferably 1 hour) and to achieve taste and concentration, it is concentrated under vacuum at low temperature (50 degrees Celsius) to prepare kelp extract with flavor, flavor and vitamins, and fermented kelp Immobilization of kelp liquor brewed using kelp [Ken liquor liquor (Cheongju or distilled liquor) produced by the method of filing for application as described in this filing patent application) and apple juice which plays a role in the fermentation of acetic acid with a catalyst well mixed in basalt Continuously brew vinegar using acetic acid bacteria carrier to produce economical and very tasty kelp vinegar To manufacture.

다시마를 추출, 농축하여 다시마 진액을 만드는 첫단계 작업을 위시하여 다시마 술을 양조하는 2 단계 작업과, 그리고 이를 식초로 발효하는 잘 자라는 초산균의 다공질 세라믹 혹은 현무압 구슬에 고정화 시키는 3 단계작업, 그리고 식초 양조에 적합한 배지를 조제하여 연속적인 초산발효로 아주 맛있는 다시마 식초를 양조하는 4 단계로 구성한다. 이를 더 상세하게 기술하고 실시 예를 추가하여 이해 를 도우기 위함인데 이 발명이 이에 국한하는것은 아니다.Two steps of brewing kelp liquor, including the first step of extracting and concentrating kelp and making kelp liquor, and three steps of immobilizing it on porous ceramics or basalt beads of acetic acid bacteria that ferment with vinegar. A medium suitable for vinegar brewing is prepared and consists of four stages of brewing a very delicious kelp vinegar with continuous acetic acid fermentation. This is to describe in more detail and to add an embodiment to help understanding but the invention is not limited thereto.

정선된 다시마를 깨끗한 물수건으로 오물을 제거하고 보통명함 넓이로 절단하여 추출탱크에 넣고 찬 식수를 10-20배 투여하여 1시간 서서히 교반 추출하여 농축기로 이송 진공상태, 섭씨50도의 환경에서 농축하여 Brix: 5.0, pH6.5 다시마액을 획득토록한다.Selected kelp is removed with clean wipes and cut into normal business area, put into the extraction tank, and 10 ~ 20 times cold drinking water is administered. The extract is stirred slowly for 1 hour and transferred to a concentrator. Concentrated in a vacuum and 50 ° C. : 5.0, pH6.5 Obtain kelp solution.

다시마 술은 분리 출원하는 다시마 술 제조방법에 따라 제조되는 다시마 청주 혹은 증류주의 주정농도를 고려하여 6-9%의 주정이 발효배지에 조제되록 준비한다.Kelp liquor is prepared to prepare 6-9% alcohol in fermentation medium in consideration of alcohol concentration of kelp liquor liquor or distilled liquor prepared according to the method for preparing kelp liquor separately.

다시마 청주를 자연 발효하여 획득된 식초로 부터 초산균주를 분리하여 다음에 명기한 초산균주 배양 액체배지를 사용하여 상온에서 48-72시간 흔들배양, 기하급수로 자라는 단계에서 멸균된 세라믹스 혹은 현무압 구슬을 투입하고 구슬담체에 초산균을 고정화 시키기위하여 계속하여 배양시켜 적정한 초산균 종균이 고정화 되도록한다.Separation of acetic acid strain from vinegar obtained by naturally fermenting kelp sake, and then sterilized ceramics or basal beads in the stage of shaking culture at room temperature for 48-72 hours using a designated acetic acid culture culture medium. Incubate and continue to incubate to immobilize the acetic acid bacteria in the bead carrier so that the appropriate acetic acid spawn is immobilized.

초산 균주 액체 배양 배지 조성: 다시마 진액 65-75%, 사과 즙: 10-20%, 주정(95%); 6-12%, 농축된 단술 4-6%.Acetic acid strain liquid culture medium composition: kelp juice 65-75%, apple juice: 10-20%, alcohol (95%); 6-12%, concentrated monotherapy 4-6%.

식초 발효는 다공질 현무암에 고정화한 초산균주의 활성이 좋을때를 포착하여 가능하면 준비된 배지를 연속 공급하여 순조롭게 초산 발효가 진행되도록한다. 즉 다시마 진액; 75%, 사과즙; 15%, 다시마 술(주정농도 95%); 6%, 맥아 농축물 3%을 미리 준비하여두고 담체에 초산균을 고정화하여 유리 칼럼에 충진하고 즉시 배양액을 위에서 중력에 의하여 흐르도록하여 초산 발효가 진행되도록한다. 고 정화한 담체의 수량에 따라 2-5개의 유리 칼럼을 상단의 발효 탱크로 부터 분활하여 하단의 발효탱크로 흐르게 하면 보다 빠른기간에 초산발효가 완료됨을 고려하고 아래 탱크에서 위 탱크로는 순환 펌프를 사용하여 배출량을 고려 상단 탱크에 고갈이 발생되지 않도록 펌프용량을 고려 설비해야한다.Vinegar fermentation captures the good activity of the acetic acid strains immobilized on the porous basalt, and if possible, continuously supplies the prepared medium so that acetic acid fermentation proceeds smoothly. Namely kelp essence; 75%, apple juice; 15% kelp liquor (95% alcohol concentration); 6% and malt concentrate 3% are prepared in advance, acetic acid bacteria are immobilized on a carrier, filled in a glass column, and the culture solution is immediately flowed by gravity from above to allow acetic acid fermentation to proceed. Depending on the number of highly purified carriers, 2-5 glass columns are split from the upper fermentation tank and flowed into the lower fermentation tank. Considering the emissions, the pump capacity should be installed to avoid exhaustion of the upper tank.

실시예 1. 다시마 추출액, 발효 매체, 및 초산균 고정화 준비와 초산 발효. Example 1. Kelp extract, fermentation medium, and acetic acid immobilization preparation and acetic acid fermentation.

정선된 다시마 10kg를 깨끗한 물수건으로 오물을 제거하고 보통명함 넓이로 절단하여 추출탱크에 넣고 찬 식수를 200릿터를 투여하여 1시간 서서히 교반 추출하여 농축기로 이송 진공상태, 섭씨50도의 환경에서 농축하여 Brix: 5.0, pH6.5 다시마액을 40릿터획득하게되었다.10kg of selected kelp is removed with clean wipes, cut into normal business area, placed in the extraction tank, and 200 liters of cold drinking water is administered and stirred slowly for 1 hour, then transferred to the concentrator. Concentrated in a vacuum and 50 degrees Celsius environment, Brix : 40 liters of 5.0 and pH6.5 kelp liquids were obtained.

분리 출원하는 다시마 술을 제조하는 방법에 따라 양조된 청주(주정 9%) 750미리릿터에 사과즙 200미리 릿터, 단술(맥아액) 50미리릿터를 혼합하여 500ml 삼각배양훌라스코에 250 미리릿터씩 나누어 넣어 4개를 준비하고, 다시마 청주에 자연 발효한 식초로 부터 분리한 초산균을 접종하여 상온에서 흔들 배양기로 3일간배양하여 초산균이 기하급수적으로 자라기 시작하는 것으로 예측되어 멸균 처리된 현무암 구슬(직경 약 2cm)를 15개 씩 넣고 계속 발효를 진행 6일째 구슬에 옮겨붙은 초산균의 상태가 구슬을 엷은 막으로 코팅한 것 같이 보여 이를 2개 유리 칼럼(3cm X 40Cm)에 옮겨 넣고 배양에 사용한 배지와 같은 배양액 5릿터를 순환시키면서 초산 발효를 진행 시킨 결과 14일이지나면서 식초함량이 4%를 넘어 15일에 구슬에 배양액이 잠기도록 남겨 두고 식초를 수학하게 되었다. 이의 성분 분석 결과는 표 1, 2와 같이 나타났으며 아주 맛이 좋은 다시마 식초로 평가 할 수 있었다.According to the method for preparing kelp liquor separately, 200 milliliters of apple juice and 50 milliliters of tangerine (malt liquor) were mixed with 750 milliliters of brewed sake (9% alcohol) and divided into 250 milliliters of 500 ml triangular culture hulasco. Prepare 4 pieces, inoculate the acetic acid bacteria isolated from naturally fermented vinegar into kelp and cultivate at room temperature for 3 days with a shake incubator to predict that the acetic acid bacteria start to grow exponentially. 2 cm) was added to each other and the fermentation continued. On the 6th day, the state of acetic acid bacteria transferred to the beads appeared to be coated with a thin film, which was transferred to two glass columns (3cm X 40Cm) and the same medium used for the culture. After fermentation of acetic acid while circulating 5 liters of culture solution, after 14 days, the vinegar content exceeded 4% and left the culture solution soaked in beads on the 15th day. I came to math. The results of the component analysis are shown in Tables 1 and 2 and could be evaluated as a very tasty kelp vinegar.

다시마 식초의 일반 성분 및 미네랄 함량(표 1)General Components and Mineral Contents of Kelp Vinegar (Table 1)

Figure 112007504297345-PAT00001
Figure 112007504297345-PAT00001

다시마 식초의 아미노산 함량 (표 2)Amino Acid Content of Kelp Vinegar (Table 2)

Figure 112007504297345-PAT00002
Figure 112007504297345-PAT00002

실시 예 2: 다시마 술로 증류한 주정을 사용한 식초 제조. Example 2: Preparation of vinegar using spirits distilled by kelp liquor.

다시마 추출액 7 릿터에 사과 즙 2 릿터, 단술 0.3릿터, 다시마 주정(95%); 0.7릿터를 혼합하여 배지로 준비하여 이미 실시예 1에서 사용한 유리칼럼에 충진된 고정화한 초산균에다 같은 요령으로 5릿터만 순환시키면서 발효를 15일간 진행 시킨 결과 실시예 1의 식초 보다 투명하면서 맛이 더 좋은 식초가 양조 되었다.7 liters of kelp extract, 2 liters of apple juice, 0.3 liters of liquor, kelp alcohol (95%); 0.7 liters were mixed and prepared as a medium, and the fermentation process was carried out for 15 days while only 5 liters were circulated with the same technique as the immobilized acetic acid bacteria filled in the glass column used in Example 1. Good vinegar was brewed.

아주 맛이 좋은 다시마 식초를 경재적이며 연속적으로 량산하는 공정과 기법을 개발하였는데 이는 공개된 다시마식초 양조 공정보다 기간이 짧고 량산이 가능하여 경재성이 우수하다. 다시마 추출액을 열처리하지 않고 짧은 시간 추출하고 저온에서 진공 농축함으로써 맛을 좋게하는 성분을 잘 보전할 수 있었으며 니끼한 맛을 주는 알긴산이 거이 함유되지 않아 깔금하고 감칠맛이 나는 다시마 식초가 생산될 수 있었다고 사료되며, 연이나 초산균을 다공질 구슬에 고정화하여 반복 사용할 수 있어 연속적인 생산이 가능할뿐만 아니라 일정한 품질의 제품을 경재적으로 생산이 가능하도록 기초를 수립하게되였다. 그리고 다시마에 함유된 맛을 좋게 하는 타우린, 굴루탐산, 및 아스팔긴산 등이 다시마 식초에 상당량 함유된것을 확인 할 수 있었으며 특히 하이드록시 푸로린(Hydroxyproline)은 감기에 감염되었을 때 몸이 상처를 치유하기 위하여 푸로린(Prolune)에 비타민 C를 작용시켜 만들어 치유하는데 필수 불가결한 물질인데 이것이 상당량(96.7mg%) 함유되어있어 감기 환자 치유 및 예방에 좋은 음식이될것으로 사료된다.We have developed a process and technique to produce a very tasty kelp vinegar economically and continuously, which is shorter than the published kelp vinegar brewing process. By extracting kelp extract for a short time without heat treatment and vacuum concentrating at low temperature, it was able to conserve the flavoring ingredients well, and it was possible to produce kelp and savory kelp vinegar because it contains no alginic acid, which gives a taste of taste. In addition, it can be used repeatedly by immobilizing lead or acetic acid bacteria in porous beads, and the foundation was established to enable continuous production as well as to produce products of constant quality. In addition, it was found that taurine, gurutamic acid, and aspartic acid, which enhance the taste of kelp, are contained in kelp vinegar, and especially, hydroxypurine (Hydroxyproline), the body heals wounds when cold is infected. It is indispensable for healing by making vitamin C by applying proline to it. It is considered to be a good food for healing and preventing cold patients because it contains a considerable amount (96.7mg%).

다시마를 사용하여 다시마 술을 양조하고 이의 일부를 아주 맛이 좋은 식초 양조에 활용할 수 있게 된다면 다시마의 수요가 증가하여 다시마 양식어민들에게 상당한 도움이 될 것으로 관측되고, 또 감기같은 질병은 대부분의 사람들이 시달리는 질병일이라 이를 빨리 치유할 수 있게 된다면 모든 국문들에게 큰 희소식이 되지 않을까, 그래서 다시마 식초양조에 새로운 장이 열리지 않을까 그게 기대 된다.If kelp can be used to brew a kelp liquor and use a portion of it for brewing very tasty vinegar, the demand for kelp will increase and it will be a great help for kelp farmers. This is a plagued disease, so if it can be cured quickly, it will be a great news for all Koreans, so it is hoped that a new chapter will open for vinegar production.

Claims (2)

다시마 술 즉 다시마를 사용하여 만든 청주 혹은 증류주에 사과즙을 첨가하여 혼합한 것을 배지로 준비하고 이를 초산균이 고정화된 다공질 세라믹스 혹은 현무암 구슬을 충진한 칼럼을 통과시키면서 초산발효가 진행하도록하여 양조하는 식초로 다시마의 감칠맛과 기능성을 지닌 다시마 식초의 제조 방법It is a vinegar that is prepared by adding apple juice to sake or distilled liquor made using kelp liquor as a medium, and then fermenting acetic acid while passing it through a column filled with porous ceramics or basalt beads immobilized with acetic acid bacteria. How to prepare kelp vinegar with rich taste and functionality of kelp 상기 청구항 1에서 명기한 방법을 적용하여 양조되는 다시마 식초.Kelp vinegar brewed by applying the method specified in claim 1.
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KR100914428B1 (en) * 2008-10-30 2009-09-01 이현석 Preparation method of drinking water for functional chicken and egg production treated by using natural ripening vinegar
CN102660444A (en) * 2012-05-30 2012-09-12 吕占祥 Healthcare wine and preparation method thereof
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KR101254696B1 (en) * 2010-10-26 2013-04-15 주식회사 한진산업 Method of preparing seaweed makgeolli
KR101361160B1 (en) * 2012-05-31 2014-02-27 주식회사 에이치티티엔 Method for brewing koji using phellinus linteus mycelial rice and seaweeds and method for prepairing fermented alcohol using thereof

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KR100914428B1 (en) * 2008-10-30 2009-09-01 이현석 Preparation method of drinking water for functional chicken and egg production treated by using natural ripening vinegar
CN102660444A (en) * 2012-05-30 2012-09-12 吕占祥 Healthcare wine and preparation method thereof
CN102660444B (en) * 2012-05-30 2015-07-22 吕占祥 Healthcare wine and preparation method thereof
CN105368693A (en) * 2015-11-22 2016-03-02 吴定叶 Boletus edulis nutritious rice vinegar

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