CN106173721B - A kind of soy molasses fermented tea fermented beverage and preparation method thereof - Google Patents

A kind of soy molasses fermented tea fermented beverage and preparation method thereof Download PDF

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Publication number
CN106173721B
CN106173721B CN201610504114.9A CN201610504114A CN106173721B CN 106173721 B CN106173721 B CN 106173721B CN 201610504114 A CN201610504114 A CN 201610504114A CN 106173721 B CN106173721 B CN 106173721B
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soy molasses
fermented
tea
corn
liquid
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CN106173721A (en
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徐速
程建军
董勇
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Northeast Agricultural University
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Northeast Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The present invention provides a kind of using soy molasses as bion beverage of primary raw material and preparation method thereof, belongs to technical field of beverage.Be with corn powder saccharification liquid culture black tea mother bacterial liquid then again using soy molasses as culture medium it is bacterium source with fermented tea, prepares novel characteristic soy molasses fermented tea fermented beverage.By implementing the present invention, the soy molasses, corn powder saccharification liquid and fermented tea of purifying are combined, three's effect complements each other, and the tea beverage health-care efficacy of formation is more shown, food and traditional black-tea fungus drink made of the far super simple soybean of resultant effect and corn.Simultaneously, the soy molasses fermented tea fermented beverage products fermentation time prepared is short, form is ideal, product remains the original nutrition of soybean, corn and functional component well, easy to digest and absorb, sweet mouthfeel is agreeable to the taste, and palatability is very good, target user is extremely wide, is a kind of dual function ecological health drink for having both beverage and having medical efficacy.

Description

A kind of soy molasses fermented tea fermented beverage and preparation method thereof
Technical field
The invention belongs to technical field of beverage, and in particular to a kind of using soy molasses and corn fine powder as the hair of primary raw material Ferment beverage and preparation method thereof.
Background technique
Contain a large amount of nutraceutical agents in corn, in addition to containing carbohydrate, protein, fat, carrotene Outside, also containing nutriments such as riboflavin in corn.Brainstrust has carried out nutriture value to a variety of staple foods such as corn, rice, wheat The indices of value and health-care effect compare, as a result, it has been found that, the vitamin content in corn is very high, respectively rice, wheat 5 times, 10 times, in maize flour contain a large amount of amino acid and glutathione, the side effect that anticancer drug can be inhibited to generate human body, It can also inhibit tumour growth.German nutrition and health care association research shows that, in all staple foods, the nutritive value of corn and health care Act on highest.Corn contains 7 kinds " anatrophics " i.e. calcium, glutathione, vitamin, magnesium, selenium, vitamin E and fatty acid.It is abundant Calcium can play the effect of blood pressure lowering.Carrotene contained in corn is converted into vitamin A after being absorbed by the body, and has anti- Cancer effect.Plant cellulose can accelerate the discharge of carcinogen He other poisonous substances.Natural VE then has promotion cell division, It delays senescence, the function of reducing serum cholesterol, prevent cutaneous lesions, moreover it is possible to mitigate artery sclerosis and brain function decline." life Element ", selenium is except famous in addition to " king of anticancer ", selenium also has strengthen immunity, prevents diabetes, cardiovascular and cerebrovascular disease, prevention and treatment The effects of hepatopathy, protection liver.Lutern that corn contains, zeaxanthin can fight eyes aging, stimulate brain cell, increase The mental and memory of strong man, glutathione contained in corn, is anticancer factor, it can make in conjunction with some other substance Lose carcinogenicity
Soy molasses are the by-products of ethanol water extraction process production soy protein concentrate, and dark brown, quality is sticky, character Exactly like Sugar cane molasses, therefore referred to as soy molasses.Soy molasses ingredient is complicated, both contains carbohydrate, protein, rouge The nutritional ingredients such as class, minerals, and contain isoflavones, soyabean oligosaccharides, soybean lecithin, phytosterol, soyasaponins etc. Functional components.Wherein account for about the 57.3% of its dry weight, including stachyose 18.6%, cotton containing carbohydrate in soy molasses Seed sugar 9.68%, sucrose 28.4% and a small amount of glucose, fructose, galactolipin etc..And the functional components in soy molasses Manyfold is improved in the more original soybean of concentration in soy molasses, therefore, soy molasses are the pairs for having very much Development volue Product resource.But current soy molasses application range is extremely limited, make in addition to minority makees cheap feed and raw produce is sold more It is discharged for waste liquid, not only waste of resource, but also brings environmental pollution.Its by-product of soybean molasses is adequately utilized, to it in this patent The added value of processing product has preferable promotion.
The black tea mother bacterial liquid be using corn powder saccharification liquid as carbon source, it is a variety of micro- through acetic acid bacteria, saccharomycete and lactic acid bacteria etc. Made of biological co-fermentation.Wherein, extract, active probiotic and its metabolism in black tea mother bacterial liquid containing some corn Product, these substances mainly include gluconic acid, acetic acid, glucose, fructose, protein, amino acid, vitamin, microelement, Ethyl alcohol and carbon dioxide etc..Fermented tea is also rich in the nutrients such as vitamin C, vitamin B, and containing there are many beneficial to human body micro- Biology, therefore be adjustable human physiological functions, boosts metabolism, helps digest, and prevents artery sclerosis, anticancer, health salubrity, As a popular health care drinks around the world.Fermented tea can treat a variety of chronic diseases, as hypertension, artery sclerosis, Coronary heart disease, diabetes, constipation, hemorrhoid, obesity, alopecia areata, white hair, cataract, rheumatic arthritis, gastritis, dysentery, anaemia, Riboflavin deficiency etc..
Currently, there are no a kind of drinks for combining three kinds of corn fine powder, soy molasses and fermented tea substance effects on the market Material.In addition, the utilization rate in the by-product of soybean molasses of industrial production soy protein concentrate is not high, it is most of to be discarded, thus Produce environmental pollution.It is longer at 10 days or so the time required to tradition culture black tea mother bacterial liquid, in industrial practice, expend the time More, time cost is high.In view of deficiency in the prior art, urgent need wants a kind of healthcare function more comprehensive, and resultant effect is good, right Environmental pollution is small, the low soy molasses fermented tea fermented beverage of time cost.
Summary of the invention
Aiming at the shortcomings in the prior art, the present invention provides a kind of soy molasses fermented tea fermented beverage and its preparation sides Method.Technical problem to be solved by the present invention lies in provide a kind of soy molasses that can reduce environmental pollution, increase added value, Time cost saving, health-care efficacy more show, food and traditional black-tea fungus drink made of the far super single-item of resultant effect, together When sweet mouthfeel, it is easy to digest absorb, palatability it is strong, target user is wider.
The present invention is raw material using soy molasses, to carry out through black tea mother bacterial liquid made of corn powder saccharification liquid culture fermented tea The preparation of a kind of delicious taste, balanced, with excellent health functions the soy molasses fermented tea fermented beverage of nutrition is made in fermentation Method.Soybean and corn added value can preferably be improved to the utilization of soy molasses and corn simultaneously, create it is good economical and Social benefit.
The present invention is achieved through the following technical solutions:
A kind of preparation method of soy molasses fermented tea fermented beverage, which is characterized in that raw material includes corn powder saccharification liquid, pure Soy molasses, fermented tea and the pure water of change;
Preparation method includes the following steps:
(1) black tea mother bacterial liquid culture: being mixed in proportion with pure water with corn powder saccharification liquid, stirred evenly, sterilized, cooling Black tea mother bacterial liquid culture medium is obtained afterwards, then connects fermented tea strain, and sterile culture 70h~90h is obtained red under conditions of 25 DEG C~35 DEG C Tea mother bacterial liquid;
(2) preparation of soy molasses culture medium: the soy molasses of purifying are mixed in proportion with pure water, and are stirred equal It is even, it is cooling after sterilizing;
(3) preparation of soy molasses red tea fungus fermented date liquid: after fermented tea mother liquor for fermentation 70h~90h, according to 5%~20% (v/v) inoculum concentration is inoculated into soy molasses culture medium, and sterile heat-preservation fermentation 6d~8d, obtains pH under the conditions of 25 DEG C~35 DEG C The soy molasses red tea fungus fermented date liquid being worth between 3.0~3.6;
(4) preparation of soy molasses fermented beverage: the soy molasses fermentation liquid that fermentation ends are obtained successively carries out gauze Coarse filtration, centrifugal filtration, film filtering simultaneously obtain soy molasses fermented tea fermented beverage through detection bottling.
The preparation method of the soy molasses fermented tea fermented beverage, which is characterized in that the corn powder saccharification liquid, The preparation method is as follows:
(1) raw material selects: selecting commercially available high-grade maize fine powder;
(2) it is gelatinized: the corn fine powder of a parts by weight and the water of three parts by weight being mixed, stirs evenly, is heated at 55 DEG C It is gelatinized within 30 minutes;
(3) it liquefies: adding the α-amylase of 4000U/g in the cornmeal mush that gelatinization is completed in 95 DEG C of heat preservations liquefaction 90min, obtain Corn fine powder liquefaction products;
(4) it is saccharified: corn fine powder liquefaction products is boiled into enzyme deactivation, be cooled to 65 DEG C, addition 104The carbohydrase of U/g is 65 DEG C heat preservation saccharification 120min, obtain corn fine powder saccharified liquid;
(5) filter, be centrifuged: by corn fine powder saccharified liquid, boil enzyme deactivation, through 10 layers of filtered through gauze, and through 3600r/min from The heart takes supernatant, obtains corn powder saccharification liquid.
The preparation method of the soy molasses fermented tea fermented beverage, the corn powder saccharification liquid of selection are soluble solid Content is the corn powder saccharification liquid of 24 ° of Bx.
A kind of preparation method of soy molasses fermented tea fermented beverage, the soy molasses preparation side of the purifying Method are as follows: soy molasses raw material is centrifuged 5min through 3600r/min, the soy molasses for taking supernatant that must purify.
The preparation method of the soy molasses fermented tea fermented beverage, the soy molasses of the purifying of selection are soluble solid Soy molasses of the shape object content in 45 ° of Bx~47 ° Bx.
The preparation method of the soy molasses fermented tea fermented beverage, black tea mother bacterial liquid culture described in step (1) The dosage of corn powder saccharification liquid is 20%~35% (v/v), the fermented tea strain inoculum concentration for preparing black tea mother bacterial liquid in base For 10%~15% (v/v).
The preparation method of the soy molasses fermented tea fermented beverage, soy molasses culture medium described in step (2) Weight percent containing soy molasses is 20%~30%.
The preparation method of the soy molasses fermented tea fermented beverage, in step (4), the gauze is 10 layers, described The centrifugation rate of centrifugal filtration is 2500r/min, centrifugation time 3min, and the membrane aperture that the film filtering is selected is 0.22 μm.
Fig. 1 show preparation technology flow chart of the invention.
The soy molasses fermented tea fermented beverage that the method obtains.
Compared with the existing technology, the beneficial technical effect of the present invention lies in:
1, not high in the utilization rate of the by-product of soybean molasses of industrial production soy protein concentrate, it is most of to be discarded, from And environmental pollution is produced, the present invention is that raw material production is novel using the by-product of soybean molasses of industrial production soy protein concentrate Beverage makes full use of it, not only reduces environmental pollution, also increases the added value of soy molasses.Simultaneously to soy molasses and The utilization of corn can preferably improve soybean and corn added value, create good economic and social benefit.
2, longer at 10 days or so the time required to tradition culture black tea mother bacterial liquid, black tea is made in the method applied in the present invention The time of mother bacterial liquid at 3 days or so, substantially reduces incubation time of the black tea mother bacterial liquid as seed liquor, when preferably having saved Between cost.
3, the beverage formulated using soy molasses, corn powder saccharification liquid and fermented tea all has significant guarantor to human body diseases Strong effect, after three organically combines, effect complements each other again, and the nutrition-balanced tea beverage health-care efficacy of formation is more evident It is aobvious, food and traditional black-tea fungus drink made of the far super simple soybean of resultant effect and corn, while form is ideal, mouthfeel It is sour-sweet, palatability is strong, it is easy to digest absorb, target user it is wider, be that a kind of dual function for having both beverage and having medical efficacy is raw State health beverages.
Detailed description of the invention
Fig. 1 is production technological process of the present invention.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and It is apparent.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art answer It should be appreciated that without departing from the spirit and scope of the invention can details to technical solution of the present invention and form repair Change or replace, but these modifications and replacement are fallen within the protection scope of the present invention.
Raw material sources
Soy molasses raw material: it is purchased from grand celebration Song Nen food biotechnology Co., Ltd
Fermented tea strain: it is purchased from Urumchi fermented tea research institute
The preparation of 1 24 ° of Bx corn powder saccharification liquids of embodiment
1) raw material selects: selecting commercially available high-grade maize fine powder;
2) it is gelatinized: the corn fine powder of a parts by weight and the water of three parts by weight being mixed, stirs evenly, heats 30 at 55 DEG C Minute is gelatinized;
3) liquefy: the α-amylase of by weight percentage 1% amount addition 4000U/g is 95 in the cornmeal mush that gelatinization is completed DEG C heat preservation liquefaction 90min, obtain corn fine powder liquefaction products;
4) it is saccharified: corn fine powder liquefaction products being boiled into enzyme deactivation, are cooled to 65 DEG C, by weight percentage 0.5% amount adds Add 104The carbohydrase of U/g obtains corn fine powder saccharified liquid in 65 DEG C of heat preservations saccharification 120min;
5) filter, be centrifuged: by corn fine powder saccharified liquid, boil enzyme deactivation, through 10 layers of filtered through gauze, and through 3600r/min from The heart takes supernatant, obtains the corn powder saccharification liquid that soluble solid content is 24 ° of Bx.
A kind of preparation method of the soy molasses fermented tea fermented beverage of embodiment 2
The soy molasses fermented tea fermented beverage is by corn powder saccharification liquid, the soy molasses of purifying, fermented tea and pure Water purification is prepared in accordance with the following methods, is as shown in Figure 1 preparation technology flow chart of the invention.
(1) black tea mother bacterial liquid culture
The soluble solid content that embodiment 1 is prepared is the corn powder saccharification liquid of 24 ° of Bx according to 30% (v/v's) Additive amount is mixed with pure water, is stirred evenly, and is obtained black tea mother bacterial liquid culture medium after boiling sterilization, cooling, is according to inoculum concentration 10% (v/v) accesses fermented tea strain, and sterile culture 70h under conditions of 30 DEG C obtains black tea mother bacterial liquid.
(2) preparation of soy molasses culture medium
Soy molasses raw material is centrifuged 5min through 3600r/min, and taking supernatant to obtain soluble solid content is the pure of 47 ° of Bx The soy molasses of change.The soy molasses for the purifying that the soluble solid content that 30g is added in 70mL water is 47 ° of Bx, and Even stirring, it is cooling after boiling.
(3) preparation of soy molasses red tea fungus fermented date liquid
After fermented tea mother liquor for fermentation 70h, according to the inoculum concentration of 8% (v/v), it is inoculated in soy molasses culture medium, 30 Sterile heat-preservation fermentation 6d under conditions of DEG C obtains the soy molasses red tea fungus fermented date liquid that pH value is 3.4.
(4) preparation of soy molasses fermented beverage
The soy molasses fermentation liquid obtained after fermentation is successively centrifuged through 10 layers of gauze coarse filtration, 2500r/min Soy molasses fermented tea fermented beverage is obtained after 3min filtering, 0.22 μm of film filtering and through detection bottling.
A kind of preparation method of the soy molasses fermented tea fermented beverage of embodiment 3
The soy molasses fermented tea fermented beverage is by corn powder saccharification liquid, the soy molasses of purifying, fermented tea and pure Water purification is prepared in accordance with the following methods:
(1) black tea mother bacterial liquid culture
The soluble solid content that embodiment 1 is prepared is the corn powder saccharification liquid of 24 ° of Bx according to 30% (v/v's) Additive amount is mixed with pure water, is stirred evenly, and is obtained black tea mother bacterial liquid culture medium after boiling sterilization, cooling, is according to inoculum concentration 10% (v/v) accesses fermented tea strain, and sterile culture 80h under conditions of 28 DEG C obtains black tea mother bacterial liquid.
(2) preparation of soy molasses culture medium
Soy molasses raw material is centrifuged 5min through 3600r/min, and taking supernatant to obtain soluble solid content is the pure of 45 ° of Bx The soy molasses of change.The soy molasses for the purifying that the soluble solid content that 25g is added in 80mL water is 45 ° of Bx, and Even stirring, it is cooling after boiling.
(3) preparation of soy molasses red tea fungus fermented date liquid
After fermented tea mother liquor for fermentation 80h, according to the inoculum concentration of 13% (v/v), it is inoculated in soy molasses culture medium, 26 Sterile heat-preservation fermentation 7d under conditions of DEG C obtains the soy molasses red tea fungus fermented date liquid that pH value is 3.5.
(4) preparation of soy molasses fermented beverage
The soy molasses fermentation liquid obtained after fermentation is successively centrifuged through 10 layers of gauze coarse filtration, 2500r/min Soy molasses fermented tea fermented beverage is obtained after 3min filtering, 0.22 μm of film filtering and through detection bottling.
A kind of preparation method of the soy molasses fermented tea fermented beverage of embodiment 4
The soy molasses fermented tea fermented beverage is by corn powder saccharification liquid, the soy molasses of purifying, fermented tea and pure Water purification is prepared in accordance with the following methods:
(1) black tea mother bacterial liquid culture
The soluble solid content that embodiment 1 is prepared is the corn powder saccharification liquid of 24 ° of Bx according to 35% (v/v's) Additive amount is mixed with pure water, is stirred evenly, and is obtained black tea mother bacterial liquid culture medium after boiling sterilization, cooling, is according to inoculum concentration 13% (v/v) accesses fermented tea strain, and sterile culture 85h under conditions of 32 DEG C obtains black tea mother bacterial liquid.
(2) preparation of soy molasses culture medium
Soy molasses raw material is centrifuged 5min through 3600r/min, and taking supernatant to obtain soluble solid content is the pure of 46 ° of Bx The soy molasses of change.The soy molasses for the purifying that the soluble solid content that 60g is added in 160mL water is 46 ° of Bx, and Even stirring, it is cooling after boiling.
(3) preparation of soy molasses red tea fungus fermented date liquid
After fermented tea mother liquor for fermentation 85h, according to the inoculum concentration of 18% (v/v), it is inoculated in soy molasses culture medium, 32 Sterile heat-preservation fermentation 7d under conditions of DEG C obtains the soy molasses red tea fungus fermented date liquid that pH value is 3.6.
(4) preparation of soy molasses fermented beverage
The soy molasses fermentation liquid obtained after fermentation is successively centrifuged through 10 layers of gauze coarse filtration, 2500r/min 3min filtering, 0.22 μm of film filtering simultaneously obtain soy molasses fermented tea fermented beverage through detection bottling.
A kind of preparation method of the soy molasses fermented tea fermented beverage of embodiment 5
The soy molasses fermented tea fermented beverage is by corn powder saccharification liquid, the soy molasses of purifying, fermented tea and pure Water purification is prepared in accordance with the following methods:
(1) black tea mother bacterial liquid culture
The soluble solid content that embodiment 1 is prepared is the corn powder saccharification liquid of 24 ° of Bx according to 24% (v/v's) Additive amount is mixed with pure water, is stirred evenly, and is obtained black tea mother bacterial liquid culture medium after boiling sterilization, cooling, is according to inoculum concentration 15% (v/v) accesses fermented tea strain, and sterile culture 80h under conditions of 28 DEG C obtains black tea mother bacterial liquid.
(2) preparation of soy molasses culture medium
Soy molasses raw material is centrifuged 5min through 3600r/min, and taking supernatant to obtain soluble solid content is the pure of 45 ° of Bx The soy molasses of change.The soy molasses for the purifying that the soluble solid content that 90g is added in 280mL water is 45 ° of Bx, and Even stirring, it is cooling after boiling.
(3) preparation of soy molasses red tea fungus fermented date liquid
After fermented tea mother liquor for fermentation 80h, according to the inoculum concentration of 11% (v/v), it is inoculated in fermentation medium, at 28 DEG C Under the conditions of sterile heat-preservation fermentation 8d, obtain pH value be 3.3 soy molasses red tea fungus fermented date liquid.
(4) preparation of soy molasses fermented beverage
The soy molasses fermentation liquid obtained after fermentation is successively centrifuged through 10 layers of gauze coarse filtration, 2500r/min 3min filtering, 0.22 μm of film filtering simultaneously obtain soy molasses fermented tea fermented beverage through detection bottling.
The soy molasses fermented tea fermented beverage being prepared according to 1-5 the method for the embodiment of the present invention, nutrition is balanced, Form ideal, sweet mouthfeel, palatability are strong, it is easy to digest absorb, food made of the far super simple soybean of its resultant effect and corn And traditional black-tea fungus drink, while target user is wider, is a kind of dual function ecology for having both beverage and having medical efficacy Health beverages.

Claims (5)

1. a kind of preparation method of soy molasses fermented tea fermented beverage, which is characterized in that be by corn powder saccharification liquid, purifying it is big Beans molasses, fermented tea and pure water are prepared in accordance with the following methods:
(1) black tea mother bacterial liquid culture: corn powder saccharification liquid being mixed with pure water, is stirred evenly, and obtains fermented tea after sterilized, cooling Mother liquor culture medium, then fermented tea strain is accessed, sterile culture 70h~90h obtains black tea mother bacterial liquid under conditions of 25 DEG C~35 DEG C;
(2) preparation of soy molasses culture medium: the soy molasses of purifying being mixed with pure water, and are stirred evenly, cold after sterilizing But;
(3) preparation of soy molasses red tea fungus fermented date liquid: after fermented tea mother liquor for fermentation 70h~90h, according to 5%~20% (v/v) Inoculum concentration be inoculated into soy molasses culture medium, sterile heat-preservation fermentation 6d~8d, obtains pH value under the conditions of 25 DEG C~35 DEG C Soy molasses red tea fungus fermented date liquid between 3.0~3.6;
(4) preparation of soy molasses fermented beverage: the soy molasses red tea fungus fermented date liquid that fermentation ends are obtained successively carries out yarn Cloth coarse filtration, centrifugal filtration, film filtering simultaneously obtain soy molasses fermented tea fermented beverage through detection bottling;
Wherein, the corn powder saccharification liquid is prepared in accordance with the following methods:
(a) raw material selects: selecting commercially available high-grade maize fine powder;
(b) it is gelatinized: the corn fine powder of a parts by weight and the water of three parts by weight being mixed, stirred evenly, 30 points are heated at 55 DEG C Clock is gelatinized;
(c) it liquefies: adding the α-amylase of 4000U/g in the cornmeal mush that gelatinization is completed in 95 DEG C of heat preservations liquefaction 90min, obtain corn Fine powder liquefaction products;
(d) it is saccharified: corn fine powder liquefaction products is boiled into enzyme deactivation, be cooled to 65 DEG C, addition 104The carbohydrase of U/g is kept the temperature at 65 DEG C Be saccharified 120min, obtains corn fine powder saccharified liquid;
(e) it filters, be centrifuged: by corn fine powder saccharified liquid, enzyme deactivation is boiled, through 10 layers of filtered through gauze, and through 3600r/min centrifuging and taking Supernatant obtains corn powder saccharification liquid;
Wherein, the soluble solid content of the soy molasses of the purifying is in 45 ° of Bx~47 ° Bx;
Wherein, corn powder saccharification liquid additive amount is 20%~35% (v/v) in black tea mother bacterial liquid culture medium described in step (1);
Wherein, the fermented tea strain inoculum concentration that black tea mother bacterial liquid is prepared described in step (1) is 10%~15% (v/v);
Wherein, weight percent of the soy molasses culture medium containing soy molasses described in step (2) is 20%~30%.
2. a kind of preparation method of soy molasses fermented tea fermented beverage according to claim 1, which is characterized in that described The soy molasses of purifying be prepared in accordance with the following methods: soy molasses raw material is centrifuged 5min through 3600r/min, takes supernatant Soluble solid content is obtained in the soy molasses of the purifying of 45 ° of Bx~47 ° Bx.
3. the preparation method of soy molasses fermented tea fermented beverage according to claim 1, which is characterized in that the jade of selection Rice saccharified liquid is the corn powder saccharification liquid that soluble solid content is 24 ° of Bx.
4. the preparation method of soy molasses fermented tea fermented beverage according to claim 1, which is characterized in that claim Gauze employed in gauze coarse filtration described in step (4) is 10 layers in 1;Centrifugation rate is in the centrifugal filtration 2500r/min, centrifugation time 3min;The membrane aperture that the film filtering is selected is 0.22 μm.
5. the soy molasses fermented tea fermented beverage that method according to any of claims 1-4 is prepared.
CN201610504114.9A 2016-06-30 2016-06-30 A kind of soy molasses fermented tea fermented beverage and preparation method thereof Expired - Fee Related CN106173721B (en)

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CN107467460A (en) * 2017-09-20 2017-12-15 安徽三芋农业发展有限公司 A kind of manufacture craft of sweet potato syrup fermented tea fermented beverage
CN107691704A (en) * 2017-10-18 2018-02-16 中国科学院昆明植物研究所 A kind of black-tea fungus drink and preparation method thereof
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