CN109153969B - 用于测定雄性能育性状态的方法和检验试剂盒 - Google Patents
用于测定雄性能育性状态的方法和检验试剂盒 Download PDFInfo
- Publication number
- CN109153969B CN109153969B CN201780024041.6A CN201780024041A CN109153969B CN 109153969 B CN109153969 B CN 109153969B CN 201780024041 A CN201780024041 A CN 201780024041A CN 109153969 B CN109153969 B CN 109153969B
- Authority
- CN
- China
- Prior art keywords
- mode
- positioning
- sperm
- vitro
- positioning mode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000035558 fertility Effects 0.000 title claims abstract description 236
- 238000000034 method Methods 0.000 title claims abstract description 107
- 238000012360 testing method Methods 0.000 title description 9
- 230000004807 localization Effects 0.000 claims abstract description 158
- 230000008010 sperm capacitation Effects 0.000 claims abstract description 81
- 238000013507 mapping Methods 0.000 claims abstract description 6
- 238000000338 in vitro Methods 0.000 claims description 255
- 210000004027 cell Anatomy 0.000 claims description 172
- 210000000582 semen Anatomy 0.000 claims description 68
- 239000003550 marker Substances 0.000 claims description 59
- 239000012528 membrane Substances 0.000 claims description 59
- 208000000509 infertility Diseases 0.000 claims description 49
- 239000000834 fixative Substances 0.000 claims description 43
- 239000003795 chemical substances by application Substances 0.000 claims description 37
- 210000000170 cell membrane Anatomy 0.000 claims description 36
- 238000009826 distribution Methods 0.000 claims description 32
- 230000036512 infertility Effects 0.000 claims description 32
- 230000004899 motility Effects 0.000 claims description 26
- TYMABNNERDVXID-DLYFRVTGSA-N Panipenem Chemical compound C([C@@H]1[C@H](C(N1C=1C(O)=O)=O)[C@H](O)C)C=1S[C@H]1CCN(C(C)=N)C1 TYMABNNERDVXID-DLYFRVTGSA-N 0.000 claims description 24
- 229930182558 Sterol Natural products 0.000 claims description 22
- 150000003432 sterols Chemical class 0.000 claims description 22
- 235000003702 sterols Nutrition 0.000 claims description 22
- 231100000535 infertility Toxicity 0.000 claims description 19
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 18
- 229920002866 paraformaldehyde Polymers 0.000 claims description 18
- ODLHGICHYURWBS-LKONHMLTSA-N trappsol cyclo Chemical group CC(O)COC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](COCC(C)O)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)COCC(O)C)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1COCC(C)O ODLHGICHYURWBS-LKONHMLTSA-N 0.000 claims description 18
- 102000009016 Cholera Toxin Human genes 0.000 claims description 17
- 108010049048 Cholera Toxin Proteins 0.000 claims description 17
- 239000006185 dispersion Substances 0.000 claims description 16
- 229920000858 Cyclodextrin Polymers 0.000 claims description 15
- 239000007769 metal material Substances 0.000 claims description 14
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 13
- 229910001424 calcium ion Inorganic materials 0.000 claims description 13
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 12
- YZOUYRAONFXZSI-SBHWVFSVSA-N (1S,3R,5R,6R,8R,10R,11R,13R,15R,16R,18R,20R,21R,23R,25R,26R,28R,30R,31S,33R,35R,36R,37S,38R,39S,40R,41S,42R,43S,44R,45S,46R,47S,48R,49S)-5,10,15,20,25,30,35-heptakis(hydroxymethyl)-37,39,40,41,42,43,44,45,46,47,48,49-dodecamethoxy-2,4,7,9,12,14,17,19,22,24,27,29,32,34-tetradecaoxaoctacyclo[31.2.2.23,6.28,11.213,16.218,21.223,26.228,31]nonatetracontane-36,38-diol Chemical compound O([C@@H]([C@H]([C@@H]1OC)OC)O[C@H]2[C@@H](O)[C@@H]([C@@H](O[C@@H]3[C@@H](CO)O[C@@H]([C@H]([C@@H]3O)OC)O[C@@H]3[C@@H](CO)O[C@@H]([C@H]([C@@H]3OC)OC)O[C@@H]3[C@@H](CO)O[C@@H]([C@H]([C@@H]3OC)OC)O[C@@H]3[C@@H](CO)O[C@@H]([C@H]([C@@H]3OC)OC)O3)O[C@@H]2CO)OC)[C@H](CO)[C@H]1O[C@@H]1[C@@H](OC)[C@H](OC)[C@H]3[C@@H](CO)O1 YZOUYRAONFXZSI-SBHWVFSVSA-N 0.000 claims description 9
- 108090000371 Esterases Proteins 0.000 claims description 9
- 108010031186 Glycoside Hydrolases Proteins 0.000 claims description 9
- 102000005744 Glycoside Hydrolases Human genes 0.000 claims description 9
- 108090001060 Lipase Proteins 0.000 claims description 9
- 102000004882 Lipase Human genes 0.000 claims description 9
- 239000004367 Lipase Substances 0.000 claims description 9
- 101710163270 Nuclease Proteins 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 9
- 102000007562 Serum Albumin Human genes 0.000 claims description 9
- 108010071390 Serum Albumin Proteins 0.000 claims description 9
- 239000003172 expectorant agent Substances 0.000 claims description 9
- 235000019421 lipase Nutrition 0.000 claims description 9
- 229940066491 mucolytics Drugs 0.000 claims description 9
- 102000030914 Fatty Acid-Binding Human genes 0.000 claims description 8
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 8
- 102000015779 HDL Lipoproteins Human genes 0.000 claims description 8
- 108010010234 HDL Lipoproteins Proteins 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 238000004043 dyeing Methods 0.000 claims description 8
- 108091022862 fatty acid binding Proteins 0.000 claims description 8
- 230000001605 fetal effect Effects 0.000 claims description 8
- 238000002073 fluorescence micrograph Methods 0.000 claims description 8
- 239000002502 liposome Substances 0.000 claims description 8
- 150000003904 phospholipids Chemical class 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 8
- 238000010186 staining Methods 0.000 claims description 8
- 210000003954 umbilical cord Anatomy 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000003306 harvesting Methods 0.000 claims description 5
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 4
- 230000000750 progressive effect Effects 0.000 claims description 3
- 230000001850 reproductive effect Effects 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 230000019100 sperm motility Effects 0.000 claims description 3
- 238000009007 Diagnostic Kit Methods 0.000 claims 6
- 238000002360 preparation method Methods 0.000 claims 6
- 239000011148 porous material Substances 0.000 claims 1
- 230000004044 response Effects 0.000 abstract description 8
- 230000006698 induction Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 124
- 239000002609 medium Substances 0.000 description 29
- 239000010902 straw Substances 0.000 description 21
- 230000008859 change Effects 0.000 description 19
- 208000021267 infertility disease Diseases 0.000 description 18
- 230000002159 abnormal effect Effects 0.000 description 15
- 230000035935 pregnancy Effects 0.000 description 12
- 238000011534 incubation Methods 0.000 description 10
- 230000006870 function Effects 0.000 description 9
- 238000003556 assay Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000002372 labelling Methods 0.000 description 7
- 238000009612 semen analysis Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 230000009027 insemination Effects 0.000 description 5
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 5
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 4
- 239000007995 HEPES buffer Substances 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 4
- 150000002270 gangliosides Chemical class 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 210000003101 oviduct Anatomy 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000003100 immobilizing effect Effects 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 108091008672 sterol hormone receptors Proteins 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 102000009027 Albumins Human genes 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 239000007640 basal medium Substances 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000002714 localization assay Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000012800 visualization Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 210000004340 zona pellucida Anatomy 0.000 description 2
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 239000011547 Bouin solution Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- -1 KSOM Substances 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 238000001604 Rao's score test Methods 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000014447 acrosomal vesicle exocytosis Effects 0.000 description 1
- 150000001299 aldehydes Chemical group 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 229940097362 cyclodextrins Drugs 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 230000002996 emotional effect Effects 0.000 description 1
- 230000028023 exocytosis Effects 0.000 description 1
- 210000005002 female reproductive tract Anatomy 0.000 description 1
- 210000002458 fetal heart Anatomy 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- LRBQNJMCXXYXIU-QWKBTXIPSA-N gallotannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@H]2[C@@H]([C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-QWKBTXIPSA-N 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007762 localization of cell Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000816 peptidomimetic Substances 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000007261 regionalization Effects 0.000 description 1
- 230000009933 reproductive health Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/08—Sphingolipids
- G01N2405/10—Glycosphingolipids, e.g. cerebrosides, gangliosides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/367—Infertility, e.g. sperm disorder, ovulatory dysfunction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physiology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211661340.XA CN115896228A (zh) | 2016-02-17 | 2017-02-17 | 用于测定雄性能育性状态的方法和检验试剂盒 |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662296420P | 2016-02-17 | 2016-02-17 | |
| US62/296420 | 2016-02-17 | ||
| US201662322252P | 2016-04-14 | 2016-04-14 | |
| US62/322252 | 2016-04-14 | ||
| US201662328890P | 2016-04-28 | 2016-04-28 | |
| US62/328890 | 2016-04-28 | ||
| PCT/US2017/018325 WO2017143157A1 (en) | 2016-02-17 | 2017-02-17 | Methods and test kits for determining male fertility status |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211661340.XA Division CN115896228A (zh) | 2016-02-17 | 2017-02-17 | 用于测定雄性能育性状态的方法和检验试剂盒 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109153969A CN109153969A (zh) | 2019-01-04 |
| CN109153969B true CN109153969B (zh) | 2023-01-10 |
Family
ID=59561394
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780024041.6A Active CN109153969B (zh) | 2016-02-17 | 2017-02-17 | 用于测定雄性能育性状态的方法和检验试剂盒 |
| CN202211661340.XA Pending CN115896228A (zh) | 2016-02-17 | 2017-02-17 | 用于测定雄性能育性状态的方法和检验试剂盒 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211661340.XA Pending CN115896228A (zh) | 2016-02-17 | 2017-02-17 | 用于测定雄性能育性状态的方法和检验试剂盒 |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10539555B2 (ja) |
| EP (1) | EP3417051A4 (ja) |
| JP (3) | JP6941616B2 (ja) |
| KR (1) | KR102641616B1 (ja) |
| CN (2) | CN109153969B (ja) |
| AU (1) | AU2017220013A1 (ja) |
| CA (1) | CA3013944A1 (ja) |
| MA (1) | MA44256A (ja) |
| MX (3) | MX2018010003A (ja) |
| NZ (1) | NZ745219A (ja) |
| TW (2) | TWI748992B (ja) |
| WO (1) | WO2017143157A1 (ja) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019263395A1 (en) * | 2018-05-02 | 2020-11-26 | Androvia Lifesciences, Llc | Methods and test kits for determining male fertility status |
| CN109212006A (zh) * | 2018-08-24 | 2019-01-15 | 北京艾普希隆生物科技有限公司 | 一种单细胞琼脂糖凝胶电泳试剂盒 |
| CN109182255A (zh) * | 2018-10-16 | 2019-01-11 | 云南农业大学 | 一种非动物源性树鼩精子体外获能液及其应用 |
| AU2019370491A1 (en) * | 2018-11-02 | 2021-06-03 | Androvia Lifesciences, Llc | Identifying status of male fertility by determining sperm capacitation and companion collection kit |
| US20220155313A1 (en) * | 2019-03-22 | 2022-05-19 | Westfäellsche Wilhelms-Universität Münster | Catsper-activity-test for determining male infertility |
| EP3958756A4 (en) * | 2019-04-30 | 2023-02-22 | Androvia Lifesciences, Llc | USE OF THE CAP SCORES IN IDENTIFYING A PRODUCTIVE APPROACH IN MEN SUFFERING FROM VARICOCELE |
| CN111733203B (zh) * | 2020-06-02 | 2021-05-18 | 青岛复诺生物医疗有限公司 | 一种精子顶体反应检测方法 |
| CN112036384B (zh) * | 2020-11-04 | 2021-02-05 | 成都朴华科技有限公司 | 精子头部形态识别方法、装置及设备 |
| US20230093352A1 (en) * | 2021-09-21 | 2023-03-23 | Cytolutions, Llc | Sexed Sperm Bulk Separation Systems |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994086A (en) * | 1994-03-30 | 1999-11-30 | North Shore University Hospital | Method for assessing infertility by binding of mannose to sperm cells |
| WO2008142396A1 (en) * | 2007-05-18 | 2008-11-27 | Anthony Denis Maher | Methods of assessing male fertility using a uridine marker |
| WO2016044392A1 (en) * | 2014-09-17 | 2016-03-24 | Cornell University | Identifying status of male fertility by determining sperm capacitation |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6051603A (en) | 1998-01-20 | 2000-04-18 | Wayne Hughes Institute | Vanadium (IV) metallocene complexes having sperm motility inhibiting activity |
| US6835717B2 (en) | 2000-03-08 | 2004-12-28 | The Johns Hopkins University School Of Medicine | β-cyclodextrin compositions, and use to prevent transmission of sexually transmitted diseases |
| US20020064849A1 (en) | 2000-09-05 | 2002-05-30 | Herr John C. | Human soluble testicular adenylyl cyclase |
| AU2003239901A1 (en) | 2002-05-28 | 2003-12-12 | Kathy M. Ensrud | Crisp polypeptides as contraceptives and inhibitors of sperm capacitation |
| US6913932B2 (en) | 2002-08-23 | 2005-07-05 | Beckman Coulter, Inc. | Formaldehyde-ammonium salt complexes for the stabilization of blood cells |
| US8367313B2 (en) * | 2003-07-23 | 2013-02-05 | Cornell University | Method of determining sperm capacitation |
| CA2533542C (en) | 2003-07-23 | 2013-06-11 | Cornell Research Foundation, Inc. | Method of determining sperm capacitation |
| EP1733528A1 (en) | 2004-04-05 | 2006-12-20 | TELEFONAKTIEBOLAGET LM ERICSSON (publ) | Method, communication device and system for address resolution mapping in a wireless multihop ad hoc network. |
| WO2010132800A1 (en) * | 2009-05-14 | 2010-11-18 | Streck, Inc. | Specimen container, system, and method |
-
2017
- 2017-02-17 KR KR1020187026493A patent/KR102641616B1/ko active IP Right Grant
- 2017-02-17 CN CN201780024041.6A patent/CN109153969B/zh active Active
- 2017-02-17 WO PCT/US2017/018325 patent/WO2017143157A1/en active Application Filing
- 2017-02-17 CN CN202211661340.XA patent/CN115896228A/zh active Pending
- 2017-02-17 NZ NZ745219A patent/NZ745219A/en unknown
- 2017-02-17 US US15/435,875 patent/US10539555B2/en active Active
- 2017-02-17 AU AU2017220013A patent/AU2017220013A1/en not_active Abandoned
- 2017-02-17 JP JP2018543191A patent/JP6941616B2/ja active Active
- 2017-02-17 TW TW106105366A patent/TWI748992B/zh active
- 2017-02-17 MA MA044256A patent/MA44256A/fr unknown
- 2017-02-17 EP EP17753900.4A patent/EP3417051A4/en active Pending
- 2017-02-17 CA CA3013944A patent/CA3013944A1/en active Pending
- 2017-02-17 MX MX2018010003A patent/MX2018010003A/es unknown
- 2017-02-17 TW TW110145620A patent/TW202238126A/zh unknown
-
2018
- 2018-08-17 MX MX2022012411A patent/MX2022012411A/es unknown
- 2018-08-17 MX MX2022012412A patent/MX2022012412A/es unknown
-
2019
- 2019-12-13 US US16/714,319 patent/US12044677B2/en active Active
-
2020
- 2020-02-17 JP JP2020024269A patent/JP7182573B2/ja active Active
-
2021
- 2021-09-06 JP JP2021144674A patent/JP2021191295A/ja not_active Ceased
-
2024
- 2024-06-12 US US18/741,095 patent/US20240329035A1/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5994086A (en) * | 1994-03-30 | 1999-11-30 | North Shore University Hospital | Method for assessing infertility by binding of mannose to sperm cells |
| WO2008142396A1 (en) * | 2007-05-18 | 2008-11-27 | Anthony Denis Maher | Methods of assessing male fertility using a uridine marker |
| WO2016044392A1 (en) * | 2014-09-17 | 2016-03-24 | Cornell University | Identifying status of male fertility by determining sperm capacitation |
Non-Patent Citations (2)
| Title |
|---|
| Implication of Hongres1 Protein in Quassin-Induced Male Reproductive Abnormality in Rats;Obembe OO等;《Endocrinology & Metabolic Syndrome》;20140501;第3卷(第2期);1-7 * |
| Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation;Enrique Amaya等;《Journal of Visualized Experiments》;20100821(第42期);1-4 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TW202238126A (zh) | 2022-10-01 |
| US10539555B2 (en) | 2020-01-21 |
| AU2017220013A1 (en) | 2018-08-30 |
| US20170234857A1 (en) | 2017-08-17 |
| MX2022012412A (es) | 2022-10-27 |
| US20200116707A1 (en) | 2020-04-16 |
| JP2019507343A (ja) | 2019-03-14 |
| WO2017143157A1 (en) | 2017-08-24 |
| KR20180114138A (ko) | 2018-10-17 |
| CA3013944A1 (en) | 2017-08-24 |
| JP2021191295A (ja) | 2021-12-16 |
| CN115896228A (zh) | 2023-04-04 |
| US12044677B2 (en) | 2024-07-23 |
| MX2022012411A (es) | 2022-10-27 |
| JP2020072758A (ja) | 2020-05-14 |
| JP7182573B2 (ja) | 2022-12-02 |
| TWI748992B (zh) | 2021-12-11 |
| EP3417051A1 (en) | 2018-12-26 |
| JP6941616B2 (ja) | 2021-09-29 |
| MX2018010003A (es) | 2018-11-09 |
| CN109153969A (zh) | 2019-01-04 |
| US20240329035A1 (en) | 2024-10-03 |
| KR102641616B1 (ko) | 2024-02-28 |
| EP3417051A4 (en) | 2019-12-25 |
| MA44256A (fr) | 2018-12-26 |
| TW201741665A (zh) | 2017-12-01 |
| NZ745219A (en) | 2023-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109153969B (zh) | 用于测定雄性能育性状态的方法和检验试剂盒 | |
| JP7473233B2 (ja) | 精子受精能獲得を判定することによるオスの受精能状態の特定 | |
| JP7168453B2 (ja) | 哺乳類受精を達成するためのアプローチおよび授精のための期間を識別するための方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 40002338 Country of ref document: HK |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |