CN109112070B

CN109112070B - Flammulina velutipes strain

Info

Publication number: CN109112070B
Application number: CN201810880150.4A
Authority: CN
Inventors: 王波
Original assignee: Soil and Fertilizer Research Institute SAAS
Current assignee: Soil and Fertilizer Research Institute SAAS
Priority date: 2018-08-03
Filing date: 2018-08-03
Publication date: 2021-10-08
Anticipated expiration: 2038-08-03
Also published as: CN109112070A

Abstract

The patent content relates to a breeding process, a growth period, a yield, fruiting body morphological characteristics, SSR molecular markers and the like of a needle mushroom factory culture strain F5C27, wherein the strain is preserved in the China Committee for culture Collection of microorganisms with the preservation number as follows: CGMCC 14554. The bred needle mushroom (Flammulina velutipes) excellent strain F5C27 has the characteristics of short growth period, high yield, high quality and the like, and is suitable for industrial bottle cultivation.

Description

Flammulina velutipes strain

Technical Field

The invention relates to a needle mushroom strain.

Background

The golden mushroom is known as flammulina velutipes (Fr.) Sing, and is a delicious food and a better health-care food. The needle mushroom is cold in nature, sweet in taste and salty in flavor, has the effects of tonifying liver, benefiting intestines and stomach and resisting cancer, and is mainly used for treating diseases such as liver diseases, gastrointestinal inflammation, ulcer, tumors and the like. The flammulina velutipes has higher zinc content, and is helpful for preventing male prostate diseases. The needle mushroom is also a high-potassium low-sodium food, can prevent and treat hypertension, and is beneficial to the old. The content of the amino acid in the needle mushroom is very rich and higher than that in the common mushrooms, particularly the content of lysine is very high, and the lysine has the function of promoting the intelligence development of children. Recent research shows that the flammulina velutipes also has a good anticancer effect.

The flammulina velutipes is one of the main edible fungi cultivated in China and is also the variety with the largest industrial production yield, at present, the flammulina velutipes strains cultivated in China mainly come from abroad, thousands of yuan of intellectual property use cost needs to be paid, and the flammulina velutipes variety with the independent intellectual property in China is bred, so that the flammulina velutipes strain is the key for promoting the development of the edible fungi industry in China.

Disclosure of Invention

The invention provides a needle mushroom strain with short growth period, high yield and excellent quality, which is suitable for industrial bottle cultivation.

The Flammulina velutipes strain F5C27 is classified and named as Flammulina velutipes, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, has the address of No. 3 Xilu No.1 of Shangyang district, Beijing, China, and has the preservation date of 2017, 9 and 18 days, and the preservation number is as follows: CGMCC NO. 14554.

The flammulina velutipes strain F5C27 is an excellent strain screened by using parents F531 and F9231 through hybridization.

The parent used in the invention is from the institute of soil fertilizer of academy of agricultural sciences of Sichuan province.

The cross breeding method of the invention comprises the following steps: a homokaryon of F9231 is obtained by a monospore separation method, the homokaryon F9231m27 of F9231 and a binuclear of F531 are subjected to pairing hybridization to obtain a hybrid strain F5C27, and the hybrid strain is determined to be an excellent strain through 3 batches of cultivation, fruiting, screening and production tests and is suitable for industrial bottle cultivation.

Drawings

FIG. 1 shows morphological features of fruiting bodies of F5C 27.

FIG. 2 is a clustering dendrogram based on genetic similarity coefficients of 25 pairs of SSR primers.

Detailed Description

The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.

The specific implementation mode is as follows: the Flammulina velutipes strain F5C27 of the embodiment is classified and named as Flammulina velutipes, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and has the address of No. 3 Xilu-Chen No.1 of the sunward area, Beijing, China, the preservation date of 2017, 9 months and 18 days, and the preservation number of the Flammulina velutipes strain is: CGMCC NO. 14554.

1. Process for selective breeding

The parents F531 and F9231 are from the institute of soil fertilizer of agricultural academy of sciences of Sichuan province, and are materials created by a cross breeding method; a homokaryon of F9231 is obtained by a monospore separation method, the homokaryon F9231m27 of F9231 and a binuclear of F531 are subjected to pairing hybridization to obtain a hybrid strain F5C27, and the hybrid strain is determined to be an excellent strain through 3 batches of cultivation, fruiting, screening tests and production tests.

2. Growth period, yield and morphological characteristics

2.1 growth phase to yield comparison

The growth period and morphology by comparing F5C27 with F531 and F9231 are shown in table 1.

TABLE 1 comparison of growth period, yield, biological efficiency of F5C27 with parents and controls

Variety of (IV) C	Growth period (Tian)	Average yield (g/bottle)	Average biological efficiency%
				F5C27	46	517.29	161.58
F531	51	436.67	136.46
				F9231	43	348.94	109.04
FRY161 control group	51	446.63	148.10

As can be seen, F5C27 was 46 days, 5 days earlier than F531 and control FRY161, and 2 days later than F9231; the average yield is 517.29 g/bottle, and the biological efficiency is 161.58%; compared with the control FRY161, the yield is increased by 9.14 percent, compared with the parent F531, the yield is increased by 18.46 percent, and compared with the parent F9231, the yield is increased by 48.25 percent.

2.2 morphological characteristics of fruiting bodies

The fruiting body is white, is not affected by light, has hemispherical pileus, inward-rolling edge, is not easy to open, has average diameter of 0.53cm, average height of 0.35cm, medium-thick, dry and hard stipe, no fluff at base, small adhesion degree, compact stipe base, white section, average length of stipe of 16.2cm, and average diameter of 0.19cm (as shown in figure 1).

SSR molecular marker analysis

The results of analysis between 25 pairs of SSR primer pairs F5C27 and the parents show that 16 pairs of SSR primers have polymorphism, wherein F5C27 has 11 pairs of specific primers with the parents, which are respectively Fv12, Fv20, Fv22, Fv30, Fv35, Fv49, Fv53, Fv56, Fv62, Fv 71 and Fv 72, as shown in table 2, and genetic similarity coefficients and clustering analysis show that the genetic similarity coefficients between F5C27 and the parents F9231 and F531 are respectively 0.632 and 0.825, and the genetic similarity coefficients between F5C27 and the parents are respectively 0.632 and 0.825, which are true hybrids, as shown in table 3 (as shown in fig. 2).

TABLE 2 Flammulina velutipes hybrid F5C27 and SSR molecular marker analysis between parents

TABLE 3 analysis of genetic similarity coefficients between F5C27 and parents

	F5C27	F531	F9231
				F5C27	1.000
F531	0.825	1.000
				F9231	0.632	0.702	1.000

Claims

1. A strain of needle mushroom is characterized in that the strain is needle mushroom: (A)Flammulina velutipes) F5C27, deposited in the China Committee for culture Collection of microorganisms with the deposition number: CGMCC 14554.