CN109105264B - 一种杜鹃兰再生植株的快速培育方法 - Google Patents
一种杜鹃兰再生植株的快速培育方法 Download PDFInfo
- Publication number
- CN109105264B CN109105264B CN201811301934.3A CN201811301934A CN109105264B CN 109105264 B CN109105264 B CN 109105264B CN 201811301934 A CN201811301934 A CN 201811301934A CN 109105264 B CN109105264 B CN 109105264B
- Authority
- CN
- China
- Prior art keywords
- rhododendron
- culture
- illumination
- seeds
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 46
- 230000008929 regeneration Effects 0.000 title claims abstract description 15
- 238000011069 regeneration method Methods 0.000 title claims abstract description 15
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 241000245165 Rhododendron ponticum Species 0.000 title 1
- 241000208422 Rhododendron Species 0.000 claims abstract description 61
- 238000012258 culturing Methods 0.000 claims abstract description 19
- 230000006698 induction Effects 0.000 claims abstract description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 42
- 238000005286 illumination Methods 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 29
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 238000009630 liquid culture Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 230000003203 everyday effect Effects 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 229930024421 Adenine Natural products 0.000 claims description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 5
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 5
- 229960000643 adenine Drugs 0.000 claims description 5
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 5
- 235000018417 cysteine Nutrition 0.000 claims description 5
- 230000012010 growth Effects 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 239000012883 rooting culture medium Substances 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 244000205754 Colocasia esculenta Species 0.000 claims description 4
- 235000006481 Colocasia esculenta Nutrition 0.000 claims description 4
- 235000013575 mashed potatoes Nutrition 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 230000000052 comparative effect Effects 0.000 description 11
- 230000004069 differentiation Effects 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 4
- 241000846172 Cremastra appendiculata Species 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 241000732800 Cymbidium Species 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000544061 Cuculus canorus Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000846171 Cremastra Species 0.000 description 1
- 208000006877 Insect Bites and Stings Diseases 0.000 description 1
- 240000001155 Opuntia dillenii Species 0.000 description 1
- 235000006544 Opuntia dillenii Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241001250596 Pleione Species 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811301934.3A CN109105264B (zh) | 2018-11-02 | 2018-11-02 | 一种杜鹃兰再生植株的快速培育方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811301934.3A CN109105264B (zh) | 2018-11-02 | 2018-11-02 | 一种杜鹃兰再生植株的快速培育方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109105264A CN109105264A (zh) | 2019-01-01 |
CN109105264B true CN109105264B (zh) | 2022-01-11 |
Family
ID=64853707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811301934.3A Active CN109105264B (zh) | 2018-11-02 | 2018-11-02 | 一种杜鹃兰再生植株的快速培育方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109105264B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109699498B (zh) * | 2019-03-11 | 2021-09-03 | 成都大学 | 一种杜鹃兰愈伤组织的培养方法 |
CN110100733B (zh) * | 2019-05-29 | 2022-03-01 | 成都大学 | 一种以杜鹃兰组培根为外植体快速诱导杜鹃兰再生植株的方法 |
CN112655555A (zh) * | 2020-12-18 | 2021-04-16 | 武汉双螺旋生物科技有限公司 | 一种提高杜鹃兰组培苗炼苗成活率的方法 |
CN113615577B (zh) * | 2021-09-16 | 2022-09-27 | 广西壮族自治区农业科学院 | 一种增加杜鹃兰原球茎数量的方法 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1623373A (zh) * | 2004-12-20 | 2005-06-08 | 南京大学 | 杜鹃兰离体培养与快速繁殖生物技术方法 |
CN1817110A (zh) * | 2006-01-18 | 2006-08-16 | 贵州省农业科学院生物技术研究所 | 杜鹃兰的组织培养与快速繁殖方法 |
CN101138322A (zh) * | 2007-08-09 | 2008-03-12 | 贵州省生物技术研究所 | 杜鹃兰组培根状茎菌根化育苗技术 |
CN100407905C (zh) * | 2006-05-11 | 2008-08-06 | 南京大学 | 杜鹃兰人工种子制作方法 |
CN101695283A (zh) * | 2009-11-10 | 2010-04-21 | 北京师范大学 | 文心兰花梗芽组培用的培养基和组培苗繁殖方法 |
CN103651121A (zh) * | 2013-11-19 | 2014-03-26 | 安徽振扬农林生态开发有限公司 | 一种白及分化、壮苗培养基 |
CN106035088A (zh) * | 2016-06-17 | 2016-10-26 | 丽江海贝瑞生物科技有限公司 | 一种白芨组培快繁方法及运用 |
CN106171998A (zh) * | 2016-07-21 | 2016-12-07 | 贵州大学 | 一种诱导杜鹃兰原球茎芽簇增殖的方法 |
CN107873521A (zh) * | 2017-12-25 | 2018-04-06 | 绵阳市蜀创农业科技有限公司 | 一种杜鹃兰种子组培快繁方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2566868A1 (en) * | 2004-05-28 | 2005-12-15 | University Of Toledo, The | A method for producing direct in vitro flowering and viable seed from cotyledon, radicle, and leaf explants and plants produced therefrom |
JP2015535837A (ja) * | 2012-10-01 | 2015-12-17 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | 土壌処理施用方法および種子処理施用方法におけるアントラニルアミド化合物の使用 |
CN112655555A (zh) * | 2020-12-18 | 2021-04-16 | 武汉双螺旋生物科技有限公司 | 一种提高杜鹃兰组培苗炼苗成活率的方法 |
-
2018
- 2018-11-02 CN CN201811301934.3A patent/CN109105264B/zh active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1623373A (zh) * | 2004-12-20 | 2005-06-08 | 南京大学 | 杜鹃兰离体培养与快速繁殖生物技术方法 |
CN1817110A (zh) * | 2006-01-18 | 2006-08-16 | 贵州省农业科学院生物技术研究所 | 杜鹃兰的组织培养与快速繁殖方法 |
CN100407905C (zh) * | 2006-05-11 | 2008-08-06 | 南京大学 | 杜鹃兰人工种子制作方法 |
CN101138322A (zh) * | 2007-08-09 | 2008-03-12 | 贵州省生物技术研究所 | 杜鹃兰组培根状茎菌根化育苗技术 |
CN101695283A (zh) * | 2009-11-10 | 2010-04-21 | 北京师范大学 | 文心兰花梗芽组培用的培养基和组培苗繁殖方法 |
CN103651121A (zh) * | 2013-11-19 | 2014-03-26 | 安徽振扬农林生态开发有限公司 | 一种白及分化、壮苗培养基 |
CN106035088A (zh) * | 2016-06-17 | 2016-10-26 | 丽江海贝瑞生物科技有限公司 | 一种白芨组培快繁方法及运用 |
CN106171998A (zh) * | 2016-07-21 | 2016-12-07 | 贵州大学 | 一种诱导杜鹃兰原球茎芽簇增殖的方法 |
CN107873521A (zh) * | 2017-12-25 | 2018-04-06 | 绵阳市蜀创农业科技有限公司 | 一种杜鹃兰种子组培快繁方法 |
Non-Patent Citations (2)
Title |
---|
In vitro Micropropagation of Cremastra appendiculata;Mingsheng Zhang等;《2005年全国植物生长物质研讨会》;20050401;第18页 * |
山慈菇(杜鹃兰)快速繁殖技术体系的建立;廖婷婷;《中国优秀硕士学位论文全文数据库(电子期刊)农业科技辑》;20121015(第10期);第10页第2.1.1节、第15页第1段、第20页第3.2.2节、第42页第5.1节 * |
Also Published As
Publication number | Publication date |
---|---|
CN109105264A (zh) | 2019-01-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109105264B (zh) | 一种杜鹃兰再生植株的快速培育方法 | |
CN114051932A (zh) | 一种以茶树带腋芽茎段为外植体建立高效快繁体系的方法 | |
CN112273231A (zh) | 一种诱导天门冬丛生芽增殖与植株再生的方法 | |
CN100374011C (zh) | 切花百合组培种苗一次成苗的方法 | |
CN104885948A (zh) | 一种油茶子叶节直接再生植株的方法 | |
CN111657151A (zh) | 一种元宝枫快速成苗方法 | |
CN113243295B (zh) | 朱顶红组培繁育方法 | |
CN101986830B (zh) | 一种洋葱组织培养的方法 | |
CN108739408A (zh) | 一种提高大叶金丝楸有效新梢分化数量的组织培养方法 | |
CN112655555A (zh) | 一种提高杜鹃兰组培苗炼苗成活率的方法 | |
CN103155869A (zh) | 甜樱桃砧木Colt组织培养方法 | |
CN102613087A (zh) | 生物组织培养繁育考来木的方法 | |
CN1154413C (zh) | 魔芋组培苗批量化生产及组培苗栽培技术 | |
CN109362568B (zh) | 一种铁线莲“樱桃唇”的组培快繁方法 | |
CN100391333C (zh) | 安祖花无菌苗组织培养和试管苗炼苗移栽方法 | |
CN101543184A (zh) | 一种魔芋的开放式组织培养方法 | |
CN111165356B (zh) | 一种牡丹的组织培养繁殖方法 | |
CN114586684A (zh) | 一种三倍体桉树新品种“京桂一号桉”的组培快繁方法 | |
CN109548655B (zh) | 苦郎树的组织培养方法 | |
CN113016610A (zh) | 一种藜麦下胚轴离体再生方法 | |
CN112772418A (zh) | 一种秋石斛兰花芽组织培养快速繁殖的方法 | |
CN111557242A (zh) | 一种莲组培苗培养与快速扩繁的方法 | |
CN110583481A (zh) | 一种诱导辽东楤木体细胞胚发生及植株再生的方法 | |
CN110786244A (zh) | 一种杜娟兰种苗组培快繁方法 | |
CN1471812A (zh) | 柚木微繁方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240511 Address after: No. 46, Group 6, Hongyan Village, Renyi Town, Tianquan County, Ya'an City, Sichuan Province, 625500 Patentee after: Sichuan Tieli Agricultural Development Co.,Ltd. Country or region after: China Address before: 610106 Sichuan province Chengdu City Shiling Town East Patentee before: CHENGDU University Country or region before: China Patentee before: TIANQUAN COUNTY TONGCHUANG HEAVY BUILDING PLANTING FARMERS PROFESSIONAL COOPERATIVE |
|
TR01 | Transfer of patent right |