CN109089852A - A method of the algae base waterflooding cultivated plant for wastewater treatment - Google Patents
A method of the algae base waterflooding cultivated plant for wastewater treatment Download PDFInfo
- Publication number
- CN109089852A CN109089852A CN201810731538.8A CN201810731538A CN109089852A CN 109089852 A CN109089852 A CN 109089852A CN 201810731538 A CN201810731538 A CN 201810731538A CN 109089852 A CN109089852 A CN 109089852A
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- Prior art keywords
- plant
- waterflooding
- wastewater treatment
- water
- waste water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The present invention provides a kind of methods of algae base waterflooding cultivated plant for wastewater treatment, microalgae is inoculated in waste water, hyperoxia water environment is built by the oxygen that the photosynthesis of microalgae discharges, then plant is directly launched in cultivating container, the overground part of plant is partially flooded with and is cultivated in water.Since this method can be relieved waterflooding to the stress of plant, plant remains to grow under flooding condition.The waterflooding cultural method is not necessarily to fixed plant, greatlies simplify plant culture, management and harvest, while can be in waste water middle-high density cultivated plant, to improve the restoration of the ecosystem efficiency of waste water.The water planting that the present invention peomotes engineering application and crop of the plant in waste water reparation produces.
Description
Technical field
The invention belongs to plant waterflooding cultivation field, in particular to a kind of algae base waterflooding cultivated plant for wastewater treatment
Method.
Background technique
Hydroponic system has that small by such environmental effects, convenient for system regulation and easy infection soil species does not plant common disease pest
The advantages such as evil.Since water planting crops yield and quality is all apparently higher than soil planting crop, agricultural, commodities market is to scale water planting
Demand cumulative year after year.Agriculture scale water planting market is at about 5 times of past 10 years intramedullary expansion, and global economy value is total at present
About 8,000,000,000 dollars of volume.In addition, water culture technology has been used for the restoration of the ecosystem of various wastewater.One step of key of water planting crops exists
In the field planting of crop, i.e., plant basal part of stem is fixed with cystosepiment or wound with sponge strip/non-woven fabrics, enter the depth of water with control plant
Degree.Its existing main problem is: 1, fixed crop is time-consuming and laborious, easy damaged crop root；2, crop is not fixed firmly, and increases
Management difficulty；3, it is unfavorable for transplanting, management and harvest；4, when plant is repaired for waste water, high-density planting is helped to improve
Remediation efficiency, and be colonized and limit cultivation of plants density.
However, the flooding stress effect generated can seriously inhibit crop if being not fixed plant directly carries out waterflooding cultivation
Growth, crop are difficult to survive.When plant is in waterflooding state, root system of plant and overground part anoxic generate anaerobic respiration, thus
High concentration ethylene and other harmful substances (hydrogen sulfide, acetic acid, butyric acid etc.) are generated, plant growth is seriously inhibited；Third in blade
Dialdehyde (MDA) content increases, and leads to the forfeiture of cell membrane selective absorbing function and the leakage of electrolyte inside cell, influences plant
A variety of physiological activities；Meanwhile ethyl alcohol, acetaldehyde, the glucoside generated in plant can destroy active oxygen metabolism system in plant
The balance of system, superoxide dismutase (SOD) and other defence enzyme activities decline and generate a large amount of free radicals, and chloroplaset is caused to drop
Solution, crop photosynthesis are suppressed, cause the chlorosis of leaf, turn to be yellow, wither and fall, Plant death when serious.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of algae base waterflooding cultivation for wastewater treatment
The method of plant.
The purpose of the present invention is achieved through the following technical solutions: a kind of algae base waterflooding cultivation for wastewater treatment
The method of plant, includes the following steps:
(1) it prepares nutrient solution: nutrient solution is made in nutritive salt or wastewater dilution；
(2) it dispenses nutrient solution: nutrient solution is dispensed into each cultivating container；
(3) it is inoculated with green alga: microalgae is inoculated into nutrient solution with the concentration of 0.125g/L；
(4) it transplants plant: plant directly being launched in cultivating container, the overground part of plant is partially flooded in water；
(5) in 180 μm of ol m of illumination-2s-1, mean temperature is 28.3 DEG C, under conditions of relative humidity 36.7-85.2% into
Row culture.
Further, nutrient solution is made by 1:3 dilution in nutritive salt or waste water and water.
Further, the volume of the cultivating container is 3L.
Further, cultivating container is the container of light-permeable.
Further, the algae of inoculation is the green alga screened from waste water.
Further, plant will directly be launched in cultivating container when transplanting, without fixation, Activities of Some Plants overground part floods
Not in water.
The present invention has the advantages that the present invention is based on the mutual Megumi effects of the species of algae and plant, by algae and plant in same one
It is cultivated in system, the growth of the carbon dioxide promotion algae of root system of plant breathing release and photosynthesis, and the photosynthesis of algae
The oxygen of release fundamentally has additional nutrients the oxygen content in liquid, eliminates under waterflooding state low oxygen concentration to the stress of crop
Effect promotes the absorption of plant root system development, nutrient and moisture and the growth of crop.The invention has the following advantages: (1)
Without manually fixing crop, directly progress waterflooding cultivation greatlies simplify the composition and operational management of hydroponic system, reduces water
Train the cost of production；(2) in waste water middle-high density cultivated plant, to improve the remediation efficiency of waste water, plant can be conducive to and existed
Engineering application in waste water reparation.
Detailed description of the invention
Fig. 1 is the iris plant that embodiment culture obtains；A left side is control group plant, and the right side is experimental group plant.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.Without departing from the spirit and substance of the case in the present invention, to method of the invention, step
Or the modifications or substitutions that condition is done, the scope of the invention is belonged to, unless otherwise specified, technological means used in embodiment is
Conventional means familiar to those skilled in the art.
Embodiment:
The present embodiment cultivates iris with green alga-Pig raising wastewater culture systems waterflooding, naturally it is also possible to using nutritive salt come generation
For waste water.
(1) nutrient solution is prepared in two groups of cultivating containers (every group is 3, and culture vessel capacity is 3L): using water as solute,
Nutrient solution is made by 1:3 dilution proportion in waste water；
(2) it is inoculated with green alga, green alga is inoculated into the 3 of experimental group with the concentration of 0.125g/L by the green alga screened from waste water
In a cultivating container, green alga is not added in control group；
(3) it transplants crop: the iris of 18 plants (plant height is about 54cm) being directly placed into cultivating container, waterflooding height is
12cm, every container move into 3 plants of plant；
(4) two groups of cultivating containers are placed in 180 μm of ol m of illumination-2s-1, mean temperature is 28.3 DEG C, relative humidity
It is cultivated under conditions of 36.7-85.2%, the waterflooding height of plant is 12cm；Iris waterflooding Duan Ye is measured at regular intervals
Piece chlorophyll content, green alga biomass, culture solution NH4- N concentration and microbe species.After culture 22 days, iris is harvested.Such as
Shown in Fig. 1, the growing way of the iris (right figure) of experimental group is significantly better than control group (left figure).Through detecting, experimental group after waterflooding cultivation
The fresh weight average value of iris is 76.0 ± 4.3g/ plants (n=3), and control group is 48.4 ± 4.7g/ plants (n=3)；Experimental group iris
The chlorophyll content of waterflooding section blade is 48.1 ± 8.6, and control group iris is by flooding stress due to being influenced, and chlorophyll is average
Content gradually decreases down 23.8 ± 15.8 (n=3) with incubation time；After culture, the average frustule of experimental group green algae
Concentration is 1.74 × 107A/mL, waste water NH4- N concentration is respectively less than 5mg/L, and the testing result of microorganism shows when culture
Between when being 15d, institute's pathogen is all removed substantially in culture solution, the relative abundance of other microorganisms in addition to a few bacterium
Also it is remarkably decreased, and control group NH4- N concentration still keeps higher level, and mean concentration is 105.9 ± 26.1mg/L (n=3).
By embodiment it is found that the present invention is based on the species Hu Megumi of algae and crop effect, without artificial field planting, directly by crop
Waterflooding is cultivated in green alga-Pig raising wastewater cultivating system, and both algae-crops are mutually promoted, and avoid flooding stress to crop
Harm, is effectively simplified the composition and operational management of hydroponic system, reduces the cost of water planting production；Waste water is being effectively treated
Meanwhile harvesting crops and green alga, output is high, high financial profit.
Claims (5)
1. a kind of method of the algae base waterflooding cultivated plant for wastewater treatment, which comprises the steps of:
(1) it prepares nutrient solution: nutrient solution is made in nutritive salt or wastewater dilution；
(2) it dispenses nutrient solution: nutrient solution is dispensed into each cultivating container；
(3) it is inoculated with green alga: microalgae is inoculated into nutrient solution with the concentration of 0.125g/L；
(4) it transplants plant: plant directly being launched in cultivating container, the overground part of plant is partially flooded in water.
(5) in 180 μm of ol m of illumination-2s-1, mean temperature is 28.3 DEG C, is trained under conditions of relative humidity 36.7-85.2%
It supports.
2. a kind of method of the algae base waterflooding cultivated plant for wastewater treatment as described in claim 1, which is characterized in that will
Nutritive salt or waste water and water are diluted by 1:3 is made nutrient solution.
3. a kind of method of the algae base waterflooding cultivated plant for wastewater treatment as described in claim 1, which is characterized in that institute
The volume for stating cultivating container is 3L.
4. a kind of method of the algae base waterflooding cultivated plant for wastewater treatment as described in claim 1, which is characterized in that institute
State the container that cultivating container is light-permeable.
5. a kind of method of the algae base waterflooding cultivated plant for wastewater treatment as described in claim 1, which is characterized in that connect
The microalgae of kind is the green alga obtained from waste water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810731538.8A CN109089852B (en) | 2018-07-05 | 2018-07-05 | Method for cultivating plants in algae-based flooding manner for wastewater treatment |
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| CN201810731538.8A CN109089852B (en) | 2018-07-05 | 2018-07-05 | Method for cultivating plants in algae-based flooding manner for wastewater treatment |
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| CN109089852A true CN109089852A (en) | 2018-12-28 |
| CN109089852B CN109089852B (en) | 2020-07-24 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110294576A (en) * | 2019-07-29 | 2019-10-01 | 新余山海企业服务有限公司 | A kind of river ecological water source purifying raft |
| CN114906939A (en) * | 2022-06-13 | 2022-08-16 | 广西大学 | Method for treating anaerobic effluent of molasses alcohol wastewater by using desmodium GXU-A4 coupled plant hydroponic system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415322A (en) * | 2011-08-18 | 2012-04-18 | 北京市农林科学院 | Method for oxygen deficiency stress of plant root system |
| CN104719110A (en) * | 2015-02-15 | 2015-06-24 | 浙江大学 | Ecological water planting method based on crop-algae mutual growing function |
-
2018
- 2018-07-05 CN CN201810731538.8A patent/CN109089852B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415322A (en) * | 2011-08-18 | 2012-04-18 | 北京市农林科学院 | Method for oxygen deficiency stress of plant root system |
| CN104719110A (en) * | 2015-02-15 | 2015-06-24 | 浙江大学 | Ecological water planting method based on crop-algae mutual growing function |
Non-Patent Citations (5)
| Title |
|---|
| 张甲耀等: "《环境微生物 下》", 31 December 2008, 武汉大学出版社 * |
| 爱生活的喵喵: "水培绿萝这样养，着了魔似的疯长，四季常青不烂根，轻松爆盆！", 《百度》 * |
| 王艳: "《走进大自然·水生植物》", 31 May 2013, 吉林出版集团有限责任公司 * |
| 肖艳: "《园林绿化湿地水生植物》", 31 March 2017, 广东科技出版社 * |
| 邬志星: "《家庭养花完全手册》", 31 August 2006, 上海科学普及出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110294576A (en) * | 2019-07-29 | 2019-10-01 | 新余山海企业服务有限公司 | A kind of river ecological water source purifying raft |
| CN114906939A (en) * | 2022-06-13 | 2022-08-16 | 广西大学 | Method for treating anaerobic effluent of molasses alcohol wastewater by using desmodium GXU-A4 coupled plant hydroponic system |
| CN114906939B (en) * | 2022-06-13 | 2023-12-05 | 广西大学 | Method for treating molasses alcohol wastewater anaerobic effluent by using chain belt alga GXU-A4 coupled plant hydroponic system |
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| CN109089852B (en) | 2020-07-24 |
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