CN102415322A - Method for oxygen deficiency stress of plant root system - Google Patents
Method for oxygen deficiency stress of plant root system Download PDFInfo
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- CN102415322A CN102415322A CN2011102370601A CN201110237060A CN102415322A CN 102415322 A CN102415322 A CN 102415322A CN 2011102370601 A CN2011102370601 A CN 2011102370601A CN 201110237060 A CN201110237060 A CN 201110237060A CN 102415322 A CN102415322 A CN 102415322A
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Abstract
The invention discloses a method for oxygen deficiency stress of a plant root system. The method comprises the following steps of: (a) placing the plant root system in a hydroponic liquid of a vessel; and (b) covering a plant oil seal layer on the liquid level of the hydroponic liquid. According to the method for the oxygen deficiency stress of the plant root system, which is disclosed by the invention, air can be isolated from oxygen of the hydroponic liquid around the plant root system, and thus the content of dissolved oxygen around the root system is gradually reduced along with the metabolism of a plant body; the state of water logging injury of a plant can be better simulated; and the content of the dissolved oxygen around the root system can be monitored in real time.
Description
Technical field
The present invention relates to a kind of method of simulating plant damage or crop failure caused by waterlogging, the method that particularly a kind of root system of plant anoxic is coerced.
Background technology
In the prior art, there are dissolved oxygen adjusting apparatus, two flower-pot cover method and waterflooding not to have ground on the research water logging injury of plant processing method.First method can only be controlled at the dissolved oxygen in the water planting liquid within the particular concentration scope, and can not the dissolved oxygen in the water planting liquid be controlled at a stable numerical point; Though second kind of state of well having simulated damage or crop failure caused by waterlogging with the third mode is limited to the technological development level of dissolved oxygen sensing in the present soil, the very difficult moment is monitored out root system of plant dissolved oxygen content on every side.
Summary of the invention
To the deficiency that exists in the prior art; The object of the present invention is to provide a kind of well state of simulating plant damage or crop failure caused by waterlogging; The content of dissolved oxygen around the monitoring plant root system constantly again; Can also the oxygen of water planting liquid around air and the plant root is isolated, the method that dissolved oxygen content reduces along with plant corpus self metabolism gradually around the root system of plant root system of plant anoxic is coerced.
Technical scheme of the present invention is achieved in that the method that a kind of root system of plant anoxic is coerced, and it is characterized in that, comprises the steps:
(a) root system of plant is placed the water planting liquid in the container;
(b) cover plant oil oil sealing layer on the liquid level of said water planting liquid.
The method that above-mentioned root system of plant anoxic is coerced in step (b) afterwards, also comprises step (c): the content of dissolved oxygen and the concentration of regulating dissolved oxygen in the said water planting liquid around the monitoring plant root system.
The method that above-mentioned root system of plant anoxic is coerced, said vegetable oil oil sealing layer thickness is 0.2～5 centimetre.
The method that above-mentioned root system of plant anoxic is coerced, root system of plant all are submerged in the said water planting liquid.
The method that above-mentioned root system of plant anoxic is coerced, from root system of plant upwards 0.5～5 centimetre the stem of plant be immersed in the said water planting liquid.
The method that above-mentioned root system of plant anoxic is coerced utilizes the content and the utilization of dissolved oxygen on every side of dissolved oxygen sensing appearance monitoring plant root system to transfer the oxygen device in said water planting liquid, to supply with oxygen, to regulate the concentration of dissolved oxygen in the said water planting liquid.
The method that above-mentioned root system of plant anoxic is coerced comprises the steps:
(a) water planting liquid is placed container; Then root system of plant is placed the water planting liquid in the container; Dissolved oxygen sensing portion with the dissolved oxygen sensing appearance is positioned in the container with accent oxygen device again; The two ends of wireway are connected with accent oxygen device with oxygen pump respectively, and the gas dispersion pipe fluid conducting of wireway and accent oxygen device, utilize oxygen pump can control the speed of oxygenation in water planting liquid; And timer is connected with oxygen pump; Utilize timer can control the time of oxygenation in water planting liquid, the liquid level of water planting liquid does not have the gas outlet of gas dispersion pipe of toning oxygen device so that can be distributed to smoothly in the water planting liquid from the gas that the outlet of gas dispersion pipe is come out, thereby can in water planting liquid, supply with oxygen through the gas dispersion pipe;
(b) cover plant oil oil sealing layer on the liquid level of water planting liquid;
(c) utilize the dissolved oxygen sensing appearance monitoring plant root system content of dissolved oxygen on every side; Utilize the concentration of transferring the oxygen device to regulate dissolved oxygen in the said water planting liquid: when needing complement lysis oxygen in the water planting liquid; With oxygen pump, timer and switch opens, the oxygen that gets into water planting liquid through wireway and gas dispersion pipe can be dispersed in the water planting liquid smoothly; When needs stop in water planting liquid complement lysis oxygen, oxygen pump, timer and switch are closed.
The invention has the beneficial effects as follows: (1) shows through experiment repeatedly: vegetable oil oil sealing layer not only can secluding air, and plant corpus itself is not exerted an influence; The method of coercing with root system of plant anoxic of the present invention can not produce the pollutant effect plant strain growth; And it is easy to operation, economical and practical.(2) the good state of simulating plant damage or crop failure caused by waterlogging; The content of dissolved oxygen around the monitoring plant root system constantly again; Can also the oxygen of water planting liquid around air and the plant root is isolated, make root system of plant dissolved oxygen content reduction gradually on every side along with plant corpus self metabolism.(3) utilize the method that root system of plant anoxic of the present invention coerces can the simulating plant damage or crop failure caused by waterlogging, simulating plant be coerced the whole process of coercing to anoxic again from the normal condition to the hypoxemia easily.(4) can make that dissolved oxygen in the water planting liquid is minimum to remain on 0.5 milligram every liter, and highly stable; The effect phase was up to more than 12 days, can study a series of researchs about water logging injury of plant under the limiting value situation.
Description of drawings
The sketch map of method in practical application that Fig. 1 coerces for root system of plant anoxic of the present invention.
Among the figure: the 1-oxygen pump, the 2-power supply, the 3-timer, 4-dissolved oxygen sensing appearance, 41-dissolved oxygen sensing portion, the 5-container, 6-transfers oxygen device, 61-gas dispersion pipe, 7-vegetable oil oil sealing layer, 8-plant, 9-water planting liquid, 10-wireway, 101-switch.
Embodiment
In conjunction with accompanying drawing the present invention is done further explanation:
The method that present embodiment root system of plant anoxic is coerced comprises the steps:
(a) water planting liquid 9 is placed container 5; Then root system of plant is placed the water planting liquid 9 in the container 5; Dissolved oxygen sensing portion 41 with dissolved oxygen sensing appearance 4 is positioned in the container 5 with accent oxygen device 6 again; The two ends of wireway 10 are connected with accent oxygen device 6 with oxygen pump 1 respectively, and wireway 10 and gas dispersion pipe 61 fluid communication of transferring oxygen device 6, utilize oxygen pump 1 can control the speed of oxygenation in water planting liquid 9; And timer 3 is connected with oxygen pump 1; Utilize timer 3 can control the time of oxygenation in water planting liquid 9, the liquid level of water planting liquid 9 does not have the gas outlet of gas dispersion pipe 61 of toning oxygen device 6 so that can be distributed to smoothly in the water planting liquid 9 from the gas that the outlet of gas dispersion pipe 61 is come out, thereby can in water planting liquid 9, supply with oxygen through gas dispersion pipe 61; Need to prove: the degree of depth of said water planting liquid 9 is to set according to plant size and plant quantity, can flood plant root, can not flood the leaf of plant, does not have 5 centimetres of root systems to be advisable with interior; The plant 8 of experiment usefulness evenly distributes at said container 5;
(b) cover plant oil oil sealing layer 7 on the liquid level of said water planting liquid 9;
(c) make the dissolved oxygen sensing portion 41 of dissolved oxygen sensing appearance 4 be arranged in said water planting liquid 9, so that utilize the dissolved oxygen sensing appearance 4 monitoring plant root systems content of dissolved oxygen on every side; Utilize the concentration of transferring oxygen device 6 to regulate dissolved oxygen in the said water planting liquid 9; Said gas dispersion pipe 61 is two or more, and the internal diameter of said gas dispersion pipe 61 is less than the internal diameter of said wireway 10; Making it possible in said water planting liquid 9, to supply with a large amount of oxygen through said wireway 10 so also disperses can be dissolved in effectively in the said water planting liquid 9 afterwards through said gas dispersion pipe 61.Said timer 3 can be set duration of ventilation according to the experiment needs; Said oxygen pump 1 can be set the logical oxygen speed of gear adjusting of ventilation according to the time needs; On said wireway 10, switch 101 is installed so that control the break-make of gas in the said wireway 10.Power supply 2 is respectively to said oxygen pump 1 and said timer 3 power supplies.
When needing complement lysis oxygen in the said water planting liquid 9, oxygen pump 1, timer 3 and switch 101 are opened, can be dispersed in smoothly in the said water planting liquid 9 through said wireway 10 and the oxygen that said gas dispersion pipe 61 gets into said water planting liquid 9; When needs stop in said water planting liquid 9 complement lysis oxygen, said oxygen pump 1, said timer 3 and said switch 101 are closed.Utilize said oxygen pump 1 can control the speed of oxygenation in said water planting liquid 9, thereby can control the concentration of dissolved oxygen in the said water planting liquid 9; Utilize said timer 3 can control the time of oxygenation in said water planting liquid 9, thereby can satisfy the needs of various experiments; Utilize said dissolved oxygen sensing appearance 4 can monitor the dissolved oxygen in the said water planting liquid 9 constantly, minimum point is the minimum critical value of dissolved oxygen concentration in the dissolved oxygen change curve.
When needing complement lysis oxygen in the water planting liquid 9; Stopper and switch 101 that cylindrical shape is separated on the cavity are opened; Do not have vegetable oil oil sealing layer 7 because cylindrical shape is separated on water planting liquid 9 surfaces in the cavity this moment; The oxygen that gets into water planting liquid 9 through gas dispersion pipe 61 can be dispersed in the water planting liquid 9 (having solved because when having vegetable oil oil sealing layer 7 on the liquid level of water planting liquid 9, bubble floats over the technical barrier that is difficult to break between water planting liquid 9 and the vegetable oil oil sealing layer 7) smoothly; When needs stop in water planting liquid 9 complement lysis oxygen; Switch 101 cuts out; The upper end jam-pack of cylindrical shape being separated cavity with stopper; And cylindrical shape is separated cavity take off and be positioned over the water planting liquid 9 (stopper can prevent here vegetable oil gets into cylindrical shape in the process separate in the cavity) from base, should remain cylindrical shape and separate the lower end of cavity and be in the water planting liquid 9, influence supplemental oxygen in water planting liquid 9 to avoid cylindrical shape to separate getting into vegetable oil in the cavity.
In the present embodiment, said vegetable oil oil sealing layer 7 thickness are any number between 0.2～5 centimetre; All can realize the goal of the invention of the invention; Preferred said vegetable oil oil sealing layer 7 thickness are 0.3～1 centimetre.If said vegetable oil oil sealing layer 7 thickness are too little, the oil sealing effect compares relatively poor, and in use (when especially getting seedling) has outside air and contact with said water planting liquid 9.If said vegetable oil oil sealing layer 7 thickness are too big, be immersed in the vegetable oil having long axis, certain influence can be arranged the normal growth of plant corpus.
Root system of plant all is submerged in the said water planting liquid 9 in the present embodiment; Upwards having the stem of 0.5～5 centimetre plant 8 to be immersed in the said water planting liquid 9 from root system of plant (is that root system of plant all is submerged in the said water planting liquid 9; And upwards there is the stem of 0.5～5 centimetre plant 8 to be immersed in the said water planting liquid 9 from root system of plant); Like this can so that said water planting liquid 9 can the submerged plant root system one section axis of top, avoid contacting with root system of plant owing to rocking of said vegetable oil oil sealing layer 7 makes vegetable oil.
The method that present embodiment root system of plant anoxic is coerced has following advantage: (1) vegetable oil oil sealing layer 7 not only can secluding air, and plant corpus itself is not exerted an influence; The method of coercing with root system of plant anoxic of the present invention can not produce the pollutant effect plant strain growth; And it is easy to operation, economical and practical.(2) the good state of simulating plant damage or crop failure caused by waterlogging; The content of dissolved oxygen around the monitoring plant root system constantly again; Can also the oxygen of water planting liquid around air and the plant root is isolated, make root system of plant dissolved oxygen content reduction gradually on every side along with plant corpus self metabolism.(3) utilize the method that root system of plant anoxic of the present invention coerces can the simulating plant damage or crop failure caused by waterlogging, simulating plant be coerced the whole process of coercing to anoxic again from the normal condition to the hypoxemia easily.(4) method of present embodiment can make things convenient for simulating plant from the normal condition to the hypoxemia, to coerce the whole process of coercing to anoxic again; Neither can cause water planting liquid to pollute, also can not influence plant growing.Can make that dissolved oxygen in the water planting liquid is minimum to remain on 0.5 milligram every liter, and highly stable; The effect phase was up to more than 12 days, can study a series of researchs about water logging injury of plant under the limiting value situation.The residing external environment of plant can be regulated and control (such as intensity of illumination, humidity, temperature, light irradiation time etc.) as required voluntarily.Plant external environment of living in can influence the dissolved oxygen in the water planting liquid, and still, the Changing Pattern of its dissolved oxygen is roughly the same, all can make it drop to critical value (0.5 milligram every liter).
The said container 5 that method in the present embodiment is used can be selected glass or made of iron as required.In addition, said vegetable oil oil sealing layer 7 is a soya-bean oil oil sealing layer, also can be other vegetable oil.
Claims (7)
1. the root system of plant anoxic method of coercing is characterized in that, comprises the steps:
(a) root system of plant is placed the water planting liquid (9) in the container (5);
(b) cover plant oil oil sealing layer (7) on the liquid level of said water planting liquid (9).
2. the method that root system of plant anoxic according to claim 1 is coerced is characterized in that, in step (b) afterwards, also comprises step (c): the content of dissolved oxygen and the concentration of regulating dissolved oxygen in the said water planting liquid (9) around the monitoring plant root system.
3. the method that root system of plant anoxic according to claim 1 is coerced is characterized in that, said vegetable oil oil sealing layer (7) thickness is 0.2～5 centimetre.
4. the method for coercing according to the arbitrary described root system of plant anoxic of claim 1-3 is characterized in that root system of plant all is submerged in the said water planting liquid (9).
5. the method that root system of plant anoxic according to claim 4 is coerced is characterized in that, from root system of plant upwards 0.5～5 centimetre the stem of plant (8) be immersed in the said water planting liquid (9).
6. the method that root system of plant anoxic according to claim 2 is coerced; It is characterized in that; Utilize the content and the utilization of dissolved oxygen on every side of dissolved oxygen sensing appearance (4) monitoring plant root system to transfer oxygen device (6) in said water planting liquid (9), to supply with oxygen, to regulate the concentration of dissolved oxygen in the said water planting liquid (9).
7. the method that root system of plant anoxic according to claim 6 is coerced is characterized in that, comprises the steps:
(a) water planting liquid (9) is placed container (5); Then root system of plant is placed the water planting liquid (9) in the container (5); Dissolved oxygen sensing portion (41) and accent oxygen device (6) with dissolved oxygen sensing appearance (4) is positioned in the container (5) again; The two ends of wireway (10) are connected with accent oxygen device (6) with oxygen pump (1) respectively; And wireway (10) and gas dispersion pipe (61) fluid communication of transferring oxygen device (6); Utilize oxygen pump (1) can control the speed of oxygenation in water planting liquid (9), and timer (3) is connected with oxygen pump (1), utilize timer (3) can control the time of oxygenation in water planting liquid (9); The liquid level of water planting liquid (9) does not have the gas outlet of gas dispersion pipe (61) of toning oxygen device (6) so that the gas that comes out from the gas outlet of gas dispersion pipe (61) can be distributed in the water planting liquid (9) smoothly, thereby can pass through gas dispersion pipe (61)) in water planting liquid (9), supply with oxygen;
(b) cover plant oil oil sealing layer (7) on the liquid level of water planting liquid (9);
(c) utilize dissolved oxygen sensing appearance (4) the monitoring plant root system content of dissolved oxygen on every side; Utilize the concentration of transferring oxygen device (6) to regulate dissolved oxygen in the said water planting liquid (9): when needing complement lysis oxygen in the water planting liquid (9); With oxygen pump (1), timer (3) and switch (101)) open, through wireway (10) and gas dispersion pipe (61)) oxygen that gets into water planting liquid (9) can be dispersed in the water planting liquid (9) smoothly; When needs stop in water planting liquid (9) complement lysis oxygen, oxygen pump (1), timer (3) and switch (101) are closed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102370601A CN102415322B (en) | 2011-08-18 | 2011-08-18 | Method for oxygen deficiency stress of plant root system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102370601A CN102415322B (en) | 2011-08-18 | 2011-08-18 | Method for oxygen deficiency stress of plant root system |
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| CN102415322A true CN102415322A (en) | 2012-04-18 |
| CN102415322B CN102415322B (en) | 2013-01-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011102370601A Expired - Fee Related CN102415322B (en) | 2011-08-18 | 2011-08-18 | Method for oxygen deficiency stress of plant root system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103598598A (en) * | 2013-11-08 | 2014-02-26 | 安徽立泰食品科技有限公司 | Negative pressure processing method of tender meat |
| CN104807967A (en) * | 2015-05-12 | 2015-07-29 | 北京电子科技职业学院 | Device and method for simulating emission reduction effect of vegetation on gas pollutants |
| CN109089852A (en) * | 2018-07-05 | 2018-12-28 | 浙江大学 | A method of the algae base waterflooding cultivated plant for wastewater treatment |
| CN115500222A (en) * | 2022-11-17 | 2022-12-23 | 潍坊学院 | Cultivation method of high-quality tomatoes |
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2011
- 2011-08-18 CN CN2011102370601A patent/CN102415322B/en not_active Expired - Fee Related
Non-Patent Citations (4)
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| 孙艳军: "根际低氧胁迫对网纹甜瓜幼苗伤害生理的研究", 《中国优秀博硕士学位论文全文数据库(硕士)-农业科技辑》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598598A (en) * | 2013-11-08 | 2014-02-26 | 安徽立泰食品科技有限公司 | Negative pressure processing method of tender meat |
| CN104807967A (en) * | 2015-05-12 | 2015-07-29 | 北京电子科技职业学院 | Device and method for simulating emission reduction effect of vegetation on gas pollutants |
| CN104807967B (en) * | 2015-05-12 | 2016-08-24 | 北京电子科技职业学院 | A kind of vegetation is to gas pollutant emission reduction effect analog and method |
| CN109089852A (en) * | 2018-07-05 | 2018-12-28 | 浙江大学 | A method of the algae base waterflooding cultivated plant for wastewater treatment |
| CN115500222A (en) * | 2022-11-17 | 2022-12-23 | 潍坊学院 | Cultivation method of high-quality tomatoes |
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| CN102415322B (en) | 2013-01-09 |
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Granted publication date: 20130109 Termination date: 20170818 |