CN109089576A - Utilize the technique of hardwood cutting breeding zelkova schneideriana - Google Patents
Utilize the technique of hardwood cutting breeding zelkova schneideriana Download PDFInfo
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Abstract
The invention belongs to plant cuttage technical field, disclose the technique with hardwood cutting breeding zelkova schneideriana comprising following steps: cutting time selects late March to early April；Sturdy fork branch, the side shoot selected on 5 years breeder mother trees make cutting, intercept cutting length 12-15cm, rugosity 0.5-1.0cm, and at least containing 2 or more healthy and strong full axillary buds in each cutting, lower clip is trimmed to 45 degree of bevelings；It is placed in plant hormone and impregnates 6-9 hours；It is inserted into matrix of taking root, insertion matrix of taking root depth is the half of cutting length, is sprinkled profoundly water；Slotting machine is arranged in greenhouse, and temperature is 25-30 DEG C, light application time 6-8h, intensity of illumination 8000-10000Lux；It cuttage the latter moon, moves to outdoor hardening two months, checks situation of taking root.The present invention uses stiff wood spring sowing technique, and material source is extensive, and rooting rate is high.
Description
Technical field
The invention belongs to plant cuttage technical fields, and in particular to utilize the technique of hardwood cutting breeding zelkova schneideriana.
Background technique
Zelkova schneideriana also known as water elm are the deciduous tree tree species of Ulmaceae Zelkova.Arbor, up to 30 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is up to 100 centimetres；
Bark canescence is taupe gray, scales off in irregular；Annual shoot puce or sepia are dredged by pubescence, after gradually
It falls off；Leaf papery is to ground paper matter, and size shape makes a variation very big, oval, oval or ovum shape lanceolar, 2-9 centimetres long, wide 1-
4 centimetres, apex is tapering or shape of tail is tapering, base portion some slightly deflections, dilute round or shallow heart, and there is crenation sawtooth at edge, has short
Tip, 8-14 pairs of lateral vein；The concave coat of middle arteries above, below it is hairless.Petiole is 4-9 millimeters long, by pubescence.Male flower has extremely short
Stalk, about 3 millimeters of diameter, perianth is split to middle part, perianth sliver 6-7, is not waited greatly, outside is lacked by fine, soft fur, degeneration ovary；The nearly nothing of female flower
Stalk, about 1.5 millimeters of diameter, tapel 4-5, outside is by fine, soft fur, and ovary is by fine, soft fur.Drupe, above deflection, recess, the milli of diameter about 4
Rice, tool back abdomen ridge, net rib is obvious, hairless, has the perianth harbored.April at florescence, fruiting period October.Zelkova schneideriana tree performance is dignified, tall and big hero
Big, autumn leaves becomes maroon, is the fine tree species of ornamental autumn leaves.Can isolated planting, group planting park and square lawn, building is other makees front yard
Shade tree；Make scenic forest with evergreen species mixed planting；Shade tree, noise-reducing and dustproof are made in avenue system pavement, road.Zelkova schneideriana side shoot sprouts energy
Power is strong, after its trunk cutting strunk, can form a large amount of side shoot, is to make the excellent vegetable material of potted landscape, but it takes off basin or company
Basin plantation is arranged in pairs or groups in gardens or with artificial hillock, scape stone, can improve its ornamental value.
The zelkova schneideriana grain of wood is thin, and matter is hard, can be water-fast, for bridge, furniture woods；Stem hide fiber synthetic cotton and rope.Beech
Sapling phase lateral root is flourishing, long and intensive, drought-resistant barren, Gu soil, wind loading rating are strong, can be used as shelter belt tree species and water and soil is protected
Tree species are held to be promoted.Zelkova schneideriana is also used as the tree species of mixed forest, such as zelkova schneideriana and Chinese scholartree mixed forest, can make full use of sky
Between and room, can preferably play protection benefit, can enhance capacity to combat natural adversities, improve land occupation condition, sufficiently
The quality and quantity that forestry products are improved using land resource and illumination resource realizes that economic interests maximize.
Zelkova schneideriana category has 6 kinds in Asia, and China's production zelkova schneideriana, leaflet beech, water elm and 4 kinds of Taiwan beech are distributed mainly on sweet
Respectful, Shaanxi, the Hubei west and south, Hunan Sichuan, Yunnan, Guizhou, Shandong, Anhui, Jiangsu, Zhejiang, Taiwan.Zhejiang area is with light leaf
Beech are main.
The breeding of the current zelkova schneideriana in China is mainly based on seedling, however, zelkova schneideriana seedling individual difference is very big, with growing directly from seeds
Seedling breeding is difficult to keep the merit of elite stand, and this greatly limits the popularizations and application of this fine tree species of zelkova schneideriana.
Cuttage is a kind of method that application prospect is most wide in traditional asexual process.Compared with grafting, underground layering etc., it has
Have the advantages that simple and easy, reproduction speed is not limited by tree species fastly, or not breeding coefficient is higher, at low cost.With artificial synthesized growth
Element succeed in developing and to the understanding of cuttage rooting mechanism, the equipment such as artificial spraying device and autocontrol temperature, humidity, illumination
Occur, the cutting propagation of many tree species difficult to take root obtains very ten-strike.It has been well documented that both at home and abroad, many reproducting tree species are all
Technique on Cuttage Propagation nursery can be used and for production of afforesting.In recent years, with the development of Developing Clonal Forestry, in particular with children
Change theory and technology to be broken, cuttage increasingly causes the concern of countries in the world, and cuttage combines with tissue cultures and has become woods
The wooden breeding, nursery field modern technologies frame.And only understand fully the mechanism of cuttage root-taking, can just cuttage technique be made constantly to create
Newly, reproductive number and quality are improved, to adapt to new development need.
The principal element for determining cutting propagation success rate includes cuttings selects, cuttings time, hormone and cutting medium etc.
Several respects.It include inserting in spring sowing and summer in zelkova schneideriana cuttage, spring sowing uses stiff wood, and the summer, which inserts, uses spray；Stiff wood has materials amount big
Feature, but it is lower relative to shoot cutting survival rate.Cutting medium of taking root has extremely important in plant cuttage propagation
Effect, different cutting mediums have a great impact to the cuttage survival rate of cuttings.The matrix of cuttage not only includes the soil of solid
Earth river sand, perlite, vermiculite, bog moss, clinker etc. further include that the water of liquid and nutrient solution and gaseous mist are inserted.They
Retention ability, gas permeability, water penetration, heat insulating ability, antibacterial etc. all respectively have feature.The selection of various cutting mediums should be according to difference
The cuttage root-takings of tree species require it is different depending on, use, be retained with creating a kind of ventilation, good heat insulating producing upper polyhybird,
Less with or without germ, and have the environmental condition of certain fertility concurrently." Zhang Chuntao etc., the Technique on Cuttage Propagation of zelkova schneideriana are economical for document
Woods is studied 2011 " perlite, peat soil, vermiculite and river sand common in zelkova schneideriana cutting medium etc. are tested, finally
Select perlite: peat soil for 3:1 mixture as matrix, highest rooting rate can reach 76%.Chinese patent technology
" CN104186164A " discloses a kind of method of zelkova schneideriana cuttage and seedling culture, wherein cutting medium select sand and yellow soil according to
The mixture of 1:4 finds, rooting rate is only capable of reaching 50% or so by verifying.The cutting medium of the prior art exists mostly takes root
The lower defect of rate, study carefully its there may be the reason of specifically include that matrix ventilation Water-saving effect difference and phytopathogen infection
Root etc..How matrix is improved, is the prior art urgently technical issues that need to address to improve zelkova schneideriana rooting rate.
Summary of the invention
The technical issues of proposing for the prior art, the present invention improves matrix, accordingly, provides and utilizes stiff wood
The technique of cutting propagation zelkova schneideriana.
The present invention is achieved by the following technical solution:
With the technique of hardwood cutting breeding zelkova schneideriana comprising following steps:
Cutting time selects late March to early April；Sturdy fork branch, the side shoot selected on 5 years breeder mother trees make cutting, and interception is inserted
Spike length degree 12-15cm, rugosity 0.5-1.0cm, at least containing 2 or more healthy and strong full axillary buds in each cutting, lower clip is repaired
It is cut to 45 degree of bevelings；It is placed in plant hormone and impregnates 6-9 hours；It is inserted into matrix of taking root, insertion matrix of taking root depth is
The half of cutting length, sprinkles profoundly water；Slotting machine is arranged in greenhouse, and temperature is 25-30 DEG C, light application time 6-8h, illumination
Intensity is 8000-10000Lux；It cuttage the latter moon, moves to outdoor hardening two months, checks situation of taking root.
Further, the matrix of taking root includes the raw material of following parts by weight: 1-2 parts of bacteria agent, 20-30 parts of vermiculite,
10-15 parts of perlite, 10-15 parts of peat soil.
Further, the preparation method of the matrix of taking root includes the following steps: first to impregnate vermiculite into the water
30min then takes out, then is mixed evenly with bacteria agent, perlite and peat soil, is placed at sun-shading ventilating and saves.
Further, the bacteria agent is prepared in accordance with the following steps: corncob being ground, crosses 20 meshes, then
It is added in the water of 3-5 times of weight, 300rpm stirs 10min, is then allowed to stand 30min, then add and account for 30% parts by weight of corncob
Wheat bran, steam treatment 10min, then cooled to room temperature, obtains fermentation medium；By sporocytophaga myxococcides seed liquor
Fermentation medium is successively inoculated into trichoderma harzianum seed liquor and carries out fermented and cultured, and inoculum concentration is 5-7%, fermented and cultured
Temperature is 30 DEG C, time 12h, mixing speed 100rpm；Then Pseudomonas fluorescens seed liquor and wax-like bud are successively inoculated with
Spore bacillus seed liquor, inoculum concentration are 6-8%, continue fermented and cultured 12h, stop fermentation, obtain bacteria agent.
Preferably,
The group of the plant hormone is divided into 200-300mg/L IBA+200-300mg/L 6-BA.
Preferably,
The outdoor hardening condition are as follows: select the sunshade net that shading rate is 50% to carry out sunshade, temperature is maintained at 20-30 DEG C.
Preferably,
In the cuttage period, water spray 1-2 times daily, keeps cuttings fresh and slotting machine moistens；20d after cuttage uses nutrition
Liquid sprays plant blade face, nutrient composition are as follows: N 180mg/L, P 25mg/L, K 50mg/L；It is sprayed using sprayer, body
Product is 2L/m2；50d and 80d after cuttage, each repeat spraying 1 time.
Preferably,
The condition of the steam treatment are as follows: pressure 0.4MPa, temperature are 130 DEG C, time 10min.
The specifically used bacterial strain of embodiment of the present invention is sporocytophaga myxococcides ATCC10011, trichoderma harzianum
CGMCC No.8081, Pseudomonas fluorescens ATCC13525, Bacillus cercus ACCC11077.Strain of the present invention
To be commercially available from CGMCC, ATCC and other commercial sources.The present invention can also be similar using the other function of same kind
Bacterial strain complete the present invention.Bacterial strain seed liquor of the present invention can prepare according to conventional methods in the art.
Technical solution of the present invention brings a series of beneficial effect by improvements over the prior art, mainly includes following
Several aspects:
The present invention improves matrix of taking root, and cottage method is simple and feasible, can rapidly promote cuttage rooting, and raising survives
Rate.
In order to improve cutting propagation efficiency, generally technique can be inserted using spring sowing and summer in different seasons.Compared with the summer inserts,
The runner of spring sowing derives from the longer elite stand of the age of tree, from a wealth of sources, but needs hormone soaking time more long, and due to spring sowing
When, cutting metabolism is relatively slow, and pathogen is relatively fewer, can suitably reduce the content of bacteria agent in matrix；The present invention
By the way of indoor culture+outdoor hardening, so that seedlings root is flourishing, seedling body is healthy and strong, improves cuttage survival rate.
Matrix plays an important role during cuttage root-taking, and the matrix of cuttage root-taking should have heat preservation, moisturizing, lead to
The good environment of permeability.In the mixed-matrix of perlite and vermiculite, the thermal insulation property of matrix is improved, and increases moisture retention,
Further improve the rooting rate of cuttings, but be decreased obviously in average root long and quantity of taking root, the particle of perlite and hardness compared with
Greatly, and nutrient content is less, cannot effectively fix root system, be unfavorable for the growth of root system；It can be improved by adding peat soil
The above problem, effectively fixed root system, and nutrient is provided, but peat soil conductivity is higher, is unfavorable for taking root, and can breed
Phytopathogen；The present invention passes through addition bacteria agent, it is suppressed that the growth of phytopathogen provides nutriment, and contains
A large amount of fibrous matter can be retained moisturizing, improve gas permeability, additionally it is possible to reduce the conductivity of peat soil.
Sporocytophaga myxococcides are grown by primary carbon source of cellulose, can be produced with symbiosis with trichoderma harzianum
Raw a variety of biologically active enzyme systems, such as: cellulase, chitinase, zytase can effectively degraded cellulose produce
Raw sugar, moreover it is possible to inhibit the diseases such as root rot, gray mold, rotten mildew；Pseudomonas fluorescens can prevent and treat plant fungal disease,
Phenol piperazine acid can be generated, nitre pyrrole presses against the substances such as rhzomorph, pyoluteorin, being capable of antagonistic phytopathogen；Bacillus cercus
Antibiotic, bacteriocin and hydrolase etc. can be generated.
Cellulose hemicellulose component is higher in corncob, and wheat bran is low in cost, containing nitrogen source required for strain growth and
Vitamin substances, the present invention are inoculated with sporocytophaga myxococcides and trichoderma harzianum first, two kinds of bacterial strains can symbiosis, collaboration
Fermented cellulosic component, generation carbohydrate is total to the Pseudomonas fluorescens of subsequent inoculations as carbon source and Bacillus cercus uses, and four
Kind bacterial strain cooperative fermentation agricultural wastes, can provide nutrient, and be able to suppress the breeding of phytopathogen for cutting medium,
Rotting for adventitious root is avoided, to improve cuttage survival rate.
Detailed description of the invention
Fig. 1: influence of the matrix of taking root to zelkova schneideriana rooting rate；
Fig. 2: matrix of taking root averagely takes root to cuttings the influence of item number；
Fig. 3: matrix of taking root is averaged the influence of root long to cuttings.
Specific embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.Product and method of the invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified in bright content, spirit and scope or appropriate changes and combinations, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
With the technique of hardwood cutting breeding zelkova schneideriana comprising following steps:
Using water elm as research object, cutting time selects March 25, and sturdy fork branch, the side shoot selected on 5 years breeder mother trees are inserted
Item intercepts cutting length 12cm, rugosity 0.5-1.0cm, at least containing 2 or more healthy and strong full axillary buds in each cutting, under
Clip is trimmed to 45 degree of bevelings.It is impregnated 9 hours in plant hormone (200mg/L IBA+200mg/L 6-BA)；It is inserted into and takes root
In matrix, insertion matrix of taking root depth is the half of cutting length, is sprinkled profoundly water；Slotting machine is arranged in greenhouse, temperature 25-
30 DEG C, light application time 6h, intensity of illumination 10000Lux.Cuttage the latter moon, move to outdoor hardening two months, inspection is taken root
Situation, it is 50% sunshade net that outdoor hardening, which selects shading rate, and temperature is maintained at 20-30 DEG C.
In cuttage, water spray 1-2 times daily, keeps cuttings fresh and slotting machine moistens；20d after cuttage is sprayed using nutrient solution
Spill plant blade face, nutrient composition are as follows: N 180mg/L, P 25mg/L, K 50mg/L, phosphate fertilizer is with NH4H2PO4Form is added, potassium
Fertilizer is with KNO3Form is added, and nitrogenous fertilizer is divided by NH4H2PO4And KNO3Form outer, insufficient section (NH is added4)2SO4Supplement；Make
It is sprayed with sprayer, volume 2L/m2.50d and 80d after cuttage, each repeat spraying 1 time.
The matrix of taking root includes the raw material of following parts by weight: 1.5 parts of bacteria agent, 20 parts of vermiculite, 10 parts of perlite, mud
10 parts of charcoal soil；
The preparation method of the matrix of taking root includes the following steps:
First vermiculite is impregnated into 30min into the water, then taken out, then is mixed with bacteria agent, perlite and peat soil
Uniformly, it is placed at sun-shading ventilating and saves.
The bacteria agent is prepared in accordance with the following steps:
Corncob is ground, 20 meshes is crossed, is then added in the water of 3 times of weight, 300rpm stirs 10min, is then allowed to stand
30min, then the wheat bran for accounting for 30% parts by weight of corncob is added, steam treatment 10min, then cooled to room temperature, is fermented
Culture medium；Sporocytophaga myxococcides seed liquor and trichoderma harzianum seed liquor are successively inoculated into fermentation medium to ferment
Culture, inoculum concentration is 7%, and the temperature of fermented and cultured is 30 DEG C, time 12h, mixing speed 100rpm；Then it successively connects
Kind Pseudomonas fluorescens seed liquor and Bacillus cercus seed liquor, inoculum concentration are 6%, continue fermented and cultured 12h, stop hair
Ferment obtains bacteria agent；The concentration of the seed liquor of each bacterial strain is controlled 2 × 108CFU/ml；The condition of the steam treatment
Are as follows: pressure 0.4MPa, temperature are 130 DEG C, time 10min.
Embodiment 2
With the technique of hardwood cutting breeding zelkova schneideriana comprising following steps:
Using water elm as research object, cutting time selects April 5, and sturdy fork branch, the side shoot selected on 5 years breeder mother trees are inserted
Item intercepts cutting length 15cm, rugosity 0.5-1.0cm, at least containing 2 or more healthy and strong full axillary buds in each cutting, under
Clip is trimmed to 45 degree of bevelings.It is impregnated 6 hours in plant hormone (300mg/L IBA+300mg/L 6-BA)；It is inserted into and takes root
In matrix, insertion matrix of taking root depth is the half of cutting length, is sprinkled profoundly water；Slotting machine is arranged in greenhouse, temperature 25-
30 DEG C, light application time 6-8h, intensity of illumination 8000-10000Lux.Cuttage the latter moon, move to outdoor hardening two months,
Inspection is taken root situation, and it is 50% sunshade net that outdoor hardening, which selects shading rate, and temperature is maintained at 20-30 DEG C.
In cuttage, water spray 1-2 times daily, keeps cuttings fresh and slotting machine moistens；20d after cuttage is sprayed using nutrient solution
Spill plant blade face, nutrient composition are as follows: N 180mg/L, P 25mg/L, K 50mg/L, phosphate fertilizer is with NH4H2PO4Form is added, potassium
Fertilizer is with KNO3Form is added, and nitrogenous fertilizer is divided by NH4H2PO4And KNO3Form outer, insufficient section (NH is added4)2SO4Supplement；Make
It is sprayed with sprayer, volume 2L/m2.50d and 80d after cuttage, each repeat spraying 1 time.
The matrix of taking root includes the raw material of following parts by weight: 2 parts of bacteria agent, 25 parts of vermiculite, 15 parts of perlite, peat
10 parts of soil；
The preparation method of the matrix of taking root includes the following steps:
First vermiculite is impregnated into 60min into the water, then taken out, then is mixed with bacteria agent, perlite and peat soil
Uniformly, it is placed at sun-shading ventilating and saves.
The bacteria agent is prepared in accordance with the following steps:
Corncob is ground, 20 meshes is crossed, is then added in the water of 5 times of weight, 300rpm stirs 10min, is then allowed to stand
60min, then the wheat bran for accounting for 50% parts by weight of corncob is added, high steam processs 10min, then cooled to room temperature, obtains
Fermentation medium；Sporocytophaga myxococcides seed liquor and trichoderma harzianum seed liquor are successively inoculated into fermentation medium to carry out
Fermented and cultured, inoculum concentration are 5%, and the temperature of fermented and cultured is 30 DEG C, time 12h, mixing speed 100rpm；Then according to
Secondary inoculation Pseudomonas fluorescens seed liquor and Bacillus cercus seed liquor, inoculum concentration are 8%, continue fermented and cultured 12h, stop
It only ferments, obtains bacteria agent；The concentration of the seed liquor of each bacterial strain is controlled 1 × 108CFU/ml；The item of the steam treatment
Part are as follows: pressure 0.4MPa, temperature are 130 DEG C, time 10min.
Embodiment 3
The situation of taking root of zelkova schneideriana hardwood cuttings of the present invention:
Different groups are set, influence of the different matrix of taking root to taking root is detected.
Experimental group: matrix of taking root prepared by embodiment 1；
Control group 1: 20 parts of vermiculite, 10 parts of perlite, 20 parts of peat soil, remaining is the same as embodiment 1；
Control group 2: 3 parts of bacteria agent, 10 parts of perlite, 20 parts of peat soil；
Control group 3: 3 parts of bacteria agent, 20 parts of vermiculite, 10 parts of perlite；
Control group 4: 3 parts of bacteria agent, 10 parts of perlite, 20 parts of peat soil；
For the other cuttage technique of each group with embodiment 1, each group cuttings number is 200, the cuttage feelings of specific each group matrix
As shown in figure 1 to 3, compared with control group 1-4, experimental group cuttage root-taking effect is best, and radical is more longer, and root system is more sent out for condition
It reaches, fibrous root is more, and rooting rate can reach 94.0%；Control group 1 does not use bacteria agent, and rooting rate is only 63.5%, and fibrous root is less,
Root long is shorter；Control group 2-4 bacteria agent despite the use of, but various aspects index still lags behind experimental group, main cause
It is only with two kinds of combinations in vermiculite, perlite, peat soil, rooting rate is 80% or so.It can by the above results
To show being to be most suitable for zelkova schneideriana stiff wood using 10 parts of 10 parts+peat soil of 1.5 parts+vermiculite of bacteria agent, 20 parts+perlite of combination
The matrix of cuttage.
Embodiment 4
The effect test of each bacterial strain in bacteria agent of the present invention:
Each group setting:
Experimental group: matrix prepared by embodiment 2；
Control group 1: not adding sporocytophaga myxococcides seed liquor, remaining is the same as embodiment 2；
Control group 2: not adding trichoderma harzianum seed liquor, remaining is the same as embodiment 2；
Control group 3: not adding Pseudomonas fluorescens seed liquor, remaining is the same as embodiment 2；
Control group 4: not adding Bacillus cercus seed liquor, remaining is the same as embodiment 2.
Each group cuttage technique is the same as embodiment 2.Concrete outcome is shown in Table 1:
Table 1
| Group | It takes root number | Mean elements/item | Average root long/item |
| Experimental group | 192 | 3.74 | 5.32 |
| Control group 1 | 175 | 3.61 | 5.24 |
| Control group 2 | 181 | 3.57 | 5.27 |
| Control group 3 | 174 | 3.49 | 5.31 |
| Control group 4 | 172 | 3.53 | 5.18 |
Conclusion: as shown in table 1, experimental group of the present invention take root number at most, number be 192, rooting rate reaches 96%, with only with
The bacteria agent of the control group 1-4 of three kinds of bacterial strains compares, and experimental group uses four kinds of strain fermentation corncobs and wheat bran, improves
The root system quality of zelkova schneideriana cuttings, take root number, radical and each index of length reach highest；The above results illustrate selection of the present invention
Four kinds of bacterial strains can be that raw material ferment using corncob and wheat bran, synergisticing performance is good, in conjunction with vermiculite, perlite and mud
Charcoal three kinds of components of soil, can promote zelkova schneideriana cuttage rooting, improve the rooting rate of zelkova schneideriana hardwood cutting.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify to technical solution documented by previous embodiment or equivalent replacement of some of the technical features；And
These are modified or replaceed, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (8)
1. with the technique of hardwood cutting breeding zelkova schneideriana comprising following steps:
Cutting time selects late March to early April；Sturdy fork branch, the side shoot selected on 5 years breeder mother trees make cutting, and interception is inserted
Spike length degree 12-15cm, rugosity 0.5-1.0cm, at least containing 2 or more healthy and strong full axillary buds in each cutting, lower clip is repaired
It is cut to 45 degree of bevelings；It is placed in plant hormone and impregnates 6-9 hours；It is inserted into matrix of taking root, insertion matrix of taking root depth is
The half of cutting length, sprinkles profoundly water；Slotting machine is arranged in greenhouse, and temperature is 25-30 DEG C, light application time 6-8h, illumination
Intensity is 8000-10000Lux；It cuttage the latter moon, moves to outdoor hardening two months, checks situation of taking root.
2. technique according to claim 1, which is characterized in that the matrix of taking root includes the raw material of following parts by weight: raw
1-2 parts of object microbial inoculum, 20-30 parts of vermiculite, 10-15 parts of perlite, 10-15 parts of peat soil.
3. technique according to claim 2, which is characterized in that the preparation method of the matrix of taking root includes the following steps:
First vermiculite is impregnated into 30min into the water, then taken out, then is mixed evenly with bacteria agent, perlite and peat soil,
It is placed at sun-shading ventilating and saves.
4. technique according to claim 2 or 3, which is characterized in that the bacteria agent is prepared in accordance with the following steps:
Corncob is ground, 20 meshes is crossed, is then added in the water of 3-5 times of weight, 300rpm stirs 10min, is then allowed to stand
30min, then the wheat bran for accounting for 30% parts by weight of corncob is added, steam treatment 10min, then cooled to room temperature, is fermented
Culture medium；Sporocytophaga myxococcides seed liquor and trichoderma harzianum seed liquor are successively inoculated into fermentation medium to ferment
Culture, inoculum concentration are 5-7%, and the temperature of fermented and cultured is 30 DEG C, time 12h, mixing speed 100rpm；Then successively
It is inoculated with Pseudomonas fluorescens seed liquor and Bacillus cercus seed liquor, inoculum concentration is 6-8%, continue fermented and cultured 12h, stop
It only ferments, obtains bacteria agent.
5. technique according to claim 1, which is characterized in that the group of the plant hormone is divided into 200-300mg/L IBA+
200-300mg/L 6-BA。
6. technique according to claim 1, which is characterized in that the outdoor hardening condition are as follows: selecting shading rate is 50%
Sunshade net carry out sunshade, temperature is maintained at 20-30 DEG C.
7. technique according to claim 1, which is characterized in that in the cuttage period, water spray 1-2 times daily keeps inserting
Fringe is fresh and slotting machine is wet；20d after cuttage sprays plant blade face, nutrient composition are as follows: N 180mg/L, P using nutrient solution
25mg/L,K 50mg/L；It is sprayed using sprayer, volume 2L/m2；50d and 80d after cuttage, each repeat spraying 1
It is secondary.
8. technique according to claim 4, which is characterized in that the condition of the steam treatment are as follows: pressure 0.4MPa, temperature
Degree is 130 DEG C, time 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810977650.XA CN109089576A (en) | 2018-08-27 | 2018-08-27 | Utilize the technique of hardwood cutting breeding zelkova schneideriana |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810977650.XA CN109089576A (en) | 2018-08-27 | 2018-08-27 | Utilize the technique of hardwood cutting breeding zelkova schneideriana |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009011165A (en) * | 2007-06-29 | 2009-01-22 | Tokyo Electric Power Co Inc:The | Method for raising pot seedling of gramineae plant, and greening method |
| CN106106075A (en) * | 2016-06-23 | 2016-11-16 | 安徽鸿基农业科技发展有限公司 | A kind of beech tree Technique on Cuttage Propagation |
-
2018
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009011165A (en) * | 2007-06-29 | 2009-01-22 | Tokyo Electric Power Co Inc:The | Method for raising pot seedling of gramineae plant, and greening method |
| CN106106075A (en) * | 2016-06-23 | 2016-11-16 | 安徽鸿基农业科技发展有限公司 | A kind of beech tree Technique on Cuttage Propagation |
Non-Patent Citations (7)
| Title |
|---|
| 何培新: "《高级微生物学》", 31 August 2017, 中国轻工业出版社 * |
| 尚庆茂: "尚庆茂博士"蔬菜集约化穴盘育苗技术"系列讲座 第四讲 育苗基质的科学配制", 《中国蔬菜》 * |
| 张广增: "《1992-2011中国园林植物保护二十年》", 30 November 2011, 天津科学技术出版社 * |
| 张文: "榉树实用育苗技术初探", 《绿色科技》 * |
| 彭春生等: "《树状月季》", 31 December 2006, 中国农业出版社 * |
| 王德斌等: "《资源生物学》", 30 September 2008, 云南科技出版社 * |
| 魏自民等: "《生活垃圾微生物强化堆肥技术》", 31 July 2008, 中国环境科学出版社 * |
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