CN109085346A - A kind of electrochemical immunoanalytical instrument and its analysis method - Google Patents
A kind of electrochemical immunoanalytical instrument and its analysis method Download PDFInfo
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- CN109085346A CN109085346A CN201811088805.0A CN201811088805A CN109085346A CN 109085346 A CN109085346 A CN 109085346A CN 201811088805 A CN201811088805 A CN 201811088805A CN 109085346 A CN109085346 A CN 109085346A
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Abstract
The invention provides a kind of electrochemical immunoanalytical instrument and its analysis method, including separator and detection device, separator includes reaction solution pipeline, the sterile waterpipe and separating liquid pipeline being connected to reaction solution pipeline, several third electromagnet are set gradually on reaction solution pipeline, setting separation control solenoid valve on separating liquid pipeline on the right side of right end third electromagnet, the prominent sterile waterpipe of reaction solution pipe end forms protruding portion, 4th electromagnet is set on protruding portion, on-off by controlling third electromagnet keeps immunomagnetic beads mobile to reaction solution pipe end, detection device is arranged in separating liquid pipe end, detection device includes the second circulation motor being successively set on separating liquid pipeline from front to back, third giant magnetoresistance chip module, to electrode, the graphite being arranged on the tube wall opposite with the separating liquid pipeline to electrode is equipped with The 5th electromagnet is arranged in graphene sensor for alkene sensor.Analyzer described in the invention is compact-sized, and measuring accuracy is high.
Description
Technical field
The invention belongs to electrochemical immunoanalytical equipment technical field, more particularly, to a kind of electrochemical immunoanalytical
Instrument and its analysis method.
Background technique
The concentration of antigen/antibody in biofluid and its health status, physiological function, physiological period etc. have direct pass
System, so the concentration of quantitative detection antigen/antibody is significant to the mankind.Antigen/antibody detection in human biological fluids at present
Technology mainly has: ELISA technology, chemiluminescence immunoassay technology, immunochromatography technique etc., but these methods are all
There is respective advantage and defect.
As the detection technique of a new generation, electrochemical immunoanalytical determination techniques have high sensitivity, selective good, detection
The features such as quick, shows wide application prospect in fields such as biomedicine, environmental monitoring, food inspections.In recent years
Come, the research work of electrochemical detection method achieves huge progress, and performance and type have also obtained quick development, so
And there are still some shortcomings for existing electrochemical immunoanalytical instrument, as test error is larger, detection accuracy is low, on-line checking difficulty
It is big etc..
Summary of the invention
In view of this, the invention is directed to a kind of electrochemical immunoanalytical instrument and its analysis method, on solving
State technical problem.
In order to achieve the above objectives, the technical solution of the invention is achieved in that
A kind of electrochemical immunoanalytical instrument, including separator and detection device, separator include reaction solution pipeline, with
The sterile waterpipe and separating liquid pipeline of reaction solution pipeline connection, set gradually several third electromagnet on reaction solution pipeline,
Separate pipeline total solenoid valve is set on the separating liquid pipeline on the right side of right end third electromagnet, and reaction solution pipe end protrusion is sterile
Waterpipe forms protruding portion, and the 4th electromagnet is arranged on protruding portion, the on-off by controlling third electromagnet make immunomagnetic beads to
Reaction solution pipe end is mobile, and detection device is arranged in separating liquid pipe end, and detection device includes being successively set on from front to back
Second circulation motor, third giant magnetoresistance chip module on separating liquid pipeline, to electrode, and be equipped with the separating liquid pipeline to electrode
The 5th electromagnet is arranged in graphene sensor for the graphene sensor being arranged on opposite tube wall.
Further, reaction solution pipeline front end is connect with reaction unit, and reaction unit includes the reacting pipe of annular,
The liquid feeding end of reacting pipe is connected to input duct, and the outlet end of reacting pipe is connected to reaction solution pipeline, in reacting pipe
Series connection access first circulation motor, is arranged the first giant magnetoresistance chip module, opposite with first circulation motor on reacting pipe
First electromagnet is set above the reacting pipe of side, the second electricity of setting on the reaction solution pipeline close to reacting pipe export direction
Magnet.
Further, the input duct is connected to sample pipe, immunomagnetic beads pipeline and cleaning fluid pipe respectively.
Further, setting is used to that the first waste solution channel for cleaning reacting pipe waste liquid to be discharged on the reaction solution pipeline,
The second waste solution channel is set on reaction solution pipeline between sterile waterpipe and the first waste solution channel.
It further, further include controller, controller includes MCU unit, energy supply control module and signal processing module, respectively
The solenoid valve of control pipeline on-off is respectively set on pipeline, the solenoid valve, electromagnet and circulation motor in all pipelines lead to respectively
It crosses energy supply control module and is connected to MCU unit, all giant magnetoresistance chip modules pass through signal processing module, and to be connected to MCU mono-
Member, MCU unit are connect with power module.
Further, the third electromagnet is from left to right uniformly arranged on reaction solution pipeline, and third electromagnet is
Electromagnetism ring or bare electromagnet or gradient electromagnetic field mould group.
Further, the 4th electromagnet is electromagnetism ring, and protruding portion is blind pipe, and electromagnetism ring is set on blind pipe, or
4th electromagnet is bare electromagnet, and protruding portion is flat tube, and bare electromagnet is fixed in flat tube.
The analysis method of electrochemical immunoanalytical instrument, mainly including the following steps:
S1, MCU unit open sample pipe solenoid valve, immunomagnetic beads pipe electromagnetic valve and the total solenoid valve of input duct, open
First circulation motor is opened, sample solution and immunomagnetic beads solution are mixed into input duct, when immunomagnetic beads solution and sample are molten
After liquid is enough, each solenoid valve is closed；
S2, MCU unit control the on-off of the first electromagnet, and first circulation motor drives mixed solution to follow in reacting pipe
Circulation is dynamic, after completion of the reaction, closes the first electromagnet, opens the second electromagnet, and immunomagnetic beads are attracted by the second electromagnet, and first
Giant magnetoresistance chip module measures the concentration of immunomagnetic beads in reacting pipe, most of when mean intensity value is lower than given threshold b
Immunomagnetic beads are collected；
S3, the second electromagnet is closed, opens the total solenoid valve of reaction solution pipeline, sterile water is passed through and drives from cleaning fluid pipe
Immunomagnetic beads flow into reaction solution pipeline, and MCU unit controls each third electromagnet and individually or simultaneously generates magnetic field according to certain timing,
From left to right successively absorption immunomagnetic beads keep it mobile to reaction solution pipeline right end；
S4, MCU unit open the 4th electromagnet, open the total solenoid valve of separate pipeline, the second waste solution channel solenoid valve and nothing
Bacterium waterpipe solenoid valve, immunomagnetic beads enter separating liquid pipeline, and part sterile water will be redirected back into reaction liquid pipeline and from second
Waste solution channel discharge；
The signal of S5, the second giant magnetoresistance chip module acquisition immunomagnetic beads quantity, are transmitted to MCU by signal processing module
Unit opens separating liquid pipe electromagnetic valve, the nothing containing immunomagnetic beads when magnetic bead mean concentration is less than given threshold c in solution
Bacterium water enters separating liquid pipeline, closes separating liquid pipe electromagnetic valve；
S6, detection control solenoid valve is opened, opens second circulation motor, third giant magnetoresistance chip module detects the number of magnetic bead
Amount is transferred to accumulation calculating in MCU unit through signal processing module, opens the 5th electromagnet, will enter the immunomagnetic beads of pipeline
It is adsorbed in graphene sensor, when third giant magnetoresistance chip module detects that the average value of immunomagnetic beads concentration is less than default threshold
When value d, detection control solenoid valve and second circulation motor are closed, the accumulated value d at current time is saved.
Further, in the step S1, signal processing module acquires the first giant magnetoresistance chip module and detects immunomagnetic beads
The signal of quantity, signal processing module will acquire signal and be input to MCU unit progress accumulation process, when accumulated value reaches setting threshold
When value a, show that immunomagnetic beads solution is enough, closes sample pipe solenoid valve, immunomagnetic beads pipe electromagnetic valve and input duct
Total solenoid valve.
Further, in the step S3, the third electromagnet of left end first is powered, by the immune magnetic in reaction solution pipeline
Pearl is adsorbed at the pipeline among the third electromagnet of left end first, and the third electromagnet is kept to be powered, and opens left end second
Immunomagnetic beads are adsorbed onto the position among two electromagnet, disconnect the third electromagnet of left end first by third electromagnet, keep the
Two third electromagnet are powered, and immunomagnetic beads are adsorbed onto the position among second third electromagnet, successively the remaining electricity of on-off
Magnet, until immunomagnetic beads are moved to the position among the third electromagnet of right end.
Compared with the existing technology, a kind of electrochemical immunoanalytical instrument and its analysis method described in the invention have with
Lower advantage:
(1) analyzer described in the invention precisely measures immunomagnetic beads by using giant magnetoresistance chip module
Change, reduces test error, remain effective test sample to the greatest extent, improve the sensitivity of instrument, improve inspection
Efficiency is surveyed, and multiple giant magnetoresistance chip modules are arranged in equipment from front to back, by the detection to different point immunomagnetic beads, to anti-
It answers, the test error that may cause of magnetic bead is lost in separation process compensated, keep test data more accurate, improve equipment
Precision, reduce testing cost, the device structure is compact, is conducive to a wide range of promote.
(2) reaction unit described in the invention is realized by control of the controller to each solenoid valve and each electromagnet
The reaction of automation and lock out operation, controller control each electromagnet timing on-off and simultaneously cooperate first circulation motor, make to be immunized
Magnetic bead comes into full contact with sample solution, reacts more thorough, by the way that electromagnet is arranged in annular reaction pipeline exit, and meanwhile it is molten
Liquid recycle stream is dynamic, keeps the separation of immunomagnetic beads more thorough.
(3) separator described in the invention is driven immune by carrying out the control of timing on-off to multiple electromagnet
Magnetic bead is moved to reaction solution pipe end, with common liquid drive compared with, separate it is more thorough, after separate magnetic bead, to divide
Carry out back flush from pipeline, pipeline-cleaning is more thorough, with it is traditional take liquid needle to design compared with, realize online separation, point
Faster from speed, separative efficiency is improved.
(4) detection device described in the invention is detected using graphene sensor, improves the sensitive of detection device
It spends, solution constant flow in detection process, real-time is good, realizes on-line checking.
Detailed description of the invention
The attached drawing for constituting a part of the invention is used to provide to further understand the invention, present invention wound
The illustrative embodiments and their description made are used to explain the present invention creation, do not constitute the improper restriction to the invention.?
In attached drawing:
Fig. 1 is the structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of reaction unit part；
Fig. 3 is the structural block diagram for controlling reaction unit；
Fig. 4 is the structural schematic diagram of separator part；
Fig. 5 is the structural block diagram for controlling separator；
Fig. 6 is the structural schematic diagram of detection device part.
Description of symbols:
1, input duct；11, sample pipe；111, sample pipe solenoid valve；12, immunomagnetic beads pipeline；121, magnetic is immunized
Pearl pipe electromagnetic valve；13, cleaning fluid pipe；131, cleaning fluid pipe solenoid valve；14, the total solenoid valve of input duct；
2, reaction unit；21, reacting pipe；22, first circulation motor；23, the first giant magnetoresistance chip module；24, first
Electromagnet；25, the second electromagnet；
3, reaction solution pipeline；31, the first waste solution channel；311, waste solution channel solenoid valve；32, the second waste solution channel；321,
Second waste solution channel solenoid valve；33, the total solenoid valve of reaction solution pipeline；34, protruding portion；
4, separator；41, third electromagnet；42, the total solenoid valve of separate pipeline；43, sterile waterpipe；431, sterile water
Pipe electromagnetic valve；44, the 4th electromagnet；45, the second giant magnetoresistance chip module；
5, separating liquid pipeline；51, separating liquid pipe electromagnetic valve；
6, detection device；61, second circulation motor；62, third giant magnetoresistance chip module；63, to electrode；64, graphene
Sensor；65, the 5th electromagnet；66, detection control solenoid valve.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the invention can
To be combined with each other.
In the description of the invention, it is to be understood that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description the invention and simplifies description, rather than indicate
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For the limitation to the invention.In addition, term " first ", " second " etc. are used for description purposes only, and should not be understood as indicating
Or it implies relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " second " etc. are defined as a result,
Feature can explicitly or implicitly include one or more of the features.In the description of the invention, unless separately
It is described, the meaning of " plurality " is two or more.
In the description of the invention, it should be noted that unless otherwise clearly defined and limited, term " peace
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the invention.
The present invention will be described in detail below with reference to the accompanying drawings and embodiments creates.
As shown in Figure 1, a kind of electrochemical immunoanalytical instrument, including pass through the sequentially connected reaction unit 2 of pipeline, separation dress
Set 4 and detection device 6, as shown in Fig. 2, reaction unit 2 include annular reacting pipe 21, one end of reacting pipe 21 be arranged into
Liquid mouth, inlet are connected to input duct 1,1 other end of input duct respectively with sample pipe 11, immunomagnetic beads pipeline 12 and clear
Washing lotion pipeline 13 is connected to, and the solenoid valve of control pipeline opening and closing is respectively set on each pipeline, have respectively sample pipe solenoid valve 111,
Immunomagnetic beads pipe electromagnetic valve 121, cleaning fluid pipe solenoid valve 131 and the total solenoid valve 14 of input duct, reacting pipe 21 are another
End setting liquid outlet, liquid outlet are connected to reaction solution pipeline 3, the series connection access first circulation motor on the reacting pipe 21 of side
22, the first giant magnetoresistance chip module 23 is arranged on the reacting pipe 21 ipsilateral with first circulation motor 22, in the anti-of the other side
It answers and the first electromagnet 24 is set above pipeline 21, the second electromagnet 25 is set on the reaction solution pipeline 3 close to liquid outlet direction.
The first waste solution channel 31 for cleaning solution waste liquid to be discharged is set on reaction solution pipeline 3, on the first waste solution channel 31
First waste solution channel solenoid valve 311 is set, the total solenoid valve 33 of reaction solution pipeline is set on reaction solution pipeline 3, reaction solution pipeline 3
The other end connects separator 4.
As shown in figure 4, separator 4 includes the sterile waterpipe 43 being connected to 3 other end of reaction solution pipeline and separating liquid
Pipeline 5, sterile waterpipe 43 and 5 coaxial inner conductor of separating liquid pipeline and vertical with the axial line of reaction solution pipeline 3, in sterile water pipe
Second waste solution channel 32, sterile waterpipe 43, separating liquid are set on the reaction solution pipeline 3 between road 43 and the first waste solution channel 31
Sterile water pipe electromagnetic valve 431, the separating liquid pipeline for controlling each pipeline on-off are respectively set on pipeline 5 and the second waste solution channel 32
Solenoid valve 51 and the second waste solution channel solenoid valve 321, the reaction liquid pipe between the second waste solution channel 32 and sterile waterpipe 43
Several third electromagnet 41 are set on road 3, and third electromagnet 41 is connected to controller, controller energy by energy supply control module
It is enough so that immunomagnetic beads is from left to right gradually moved to reaction solution pipeline 3 according to the on-off of certain timing control third electromagnet 41
The total solenoid valve 42 of separate pipeline is arranged on the separating liquid pipeline 5 on the right side of right end third electromagnet 41 in end.
The prominent sterile waterpipe 43 in 3 end of reaction solution pipeline forms protruding portion 34, and the 4th electromagnet is arranged on protruding portion 34
44, immunomagnetic beads can be adsorbed on the side wall of the sterile waterpipe 43 joined with reaction solution pipeline 3 by the 4th electromagnet 44, nothing
Sterile water is passed through in bacterium waterpipe 43 to clean immunomagnetic beads, and the second giant magnetoresistance chip dies are arranged on separating liquid pipeline 5
Group 45, the second giant magnetoresistance chip module 45 is connected to controller.
Third electromagnet 41 is from left to right uniform to be arranged on reaction solution pipeline 3, third electromagnet 41 be electromagnetism ring or
Person's bare electromagnet or gradient electromagnetic field mould group.
4th electromagnet 44 is electromagnetism ring, and protruding portion 34 is blind pipe, and electromagnetism ring is set on blind pipe or the 4th electromagnet
44 be bare electromagnet, and protruding portion 34 is flat tube, and bare electromagnet is fixed in flat tube.
Detection device 6 is arranged in the other end of separating liquid pipeline 5, as shown in fig. 6, detection device 6 includes being connected on separating liquid
Second circulation motor 61 in pipeline 5 sets gradually detection control electricity on the separating liquid pipeline 5 at 61 rear of second circulation motor
Magnet valve 66 and third giant magnetoresistance chip module 62, on the upper wall of the separating liquid pipeline 5 at 62 rear of third giant magnetoresistance chip module
And graphene sensor is arranged on the lower wall for being equipped with the separating liquid pipeline 5 opposite to 63 middle position of electrode to electrode 63 in setting
64, the 5th electromagnet 65 is set below graphene sensor 64, the setting opposite to electrode 63 and graphene sensor 64 point
On the tube wall of chaotropic pipeline 5, the end of separating liquid pipeline 5 is connect with waste collecting device, and detection device 6 further includes electrification
Work station is learned, electrochemical workstation is connected to controller.
The quantity of third electromagnet 41 can depending on the specification of pipeline, separator 4 can series multistage use to increase
Add the wash number to immunomagnetic beads.
As shown in Figure 3 and Figure 5, which further includes controller, and controller includes MCU unit, energy supply control module and letter
Number processing module, solenoid valve, electromagnet and circulation motor in all pipelines pass through energy supply control module to be respectively connected to MCU mono-
Member, all giant magnetoresistance chip modules, electrochemical workstation pass through signal processing module and are connected to MCU unit, MCU unit with
Power module connection, power module provide electric energy for MCU unit.
The analysis method of electrochemical immunoanalytical instrument, mainly including the following steps:
Reaction process: drive immunomagnetic beads in reacting pipe 21 using first circulation motor 22, electromagnet, each solenoid valve
It mixes, reaction, using the on-off enrichment and separation magnetic bead of electromagnet and solenoid valve, is detected using the first giant magnetoresistance chip module 23
The quantity of immunomagnetic beads cleans pipeline using cleaning solution, collects immunomagnetic beads.
MCU unit opens sample pipe solenoid valve 111, immunomagnetic beads pipe electromagnetic valve 121 and the total solenoid valve of input duct
14, first circulation motor 22 is opened, sample solution and immunomagnetic beads solution are mixed into input duct 1, by T1 time, sample-adding
It finishes, closes sample pipe solenoid valve 111, at the same time, signal processing module acquires the detection of the first giant magnetoresistance chip module 23
The signal of immunomagnetic beads quantity, signal processing module will acquire signal and be input to MCU unit progress accumulation process, when accumulated value reaches
When to given threshold a, immunomagnetic beads solution is enough, closes immunomagnetic beads pipe electromagnetic valve 121 and the total solenoid valve of input duct
14。
Using T2 as the on-off of the first electromagnet of time interval controls 24, first circulation motor 22 quickly drives mixed MCU unit
It closes solution to circulate in reacting pipe 21, the interval on-off of the first electromagnet 24 can change immunomagnetic beads in the solution
Motion profile forms turbulent flow, so that immunomagnetic beads and sample solution is reacted more abundant, after the T3 time, end of reaction is closed
Close the first electromagnet 24.
MCU unit opens the second electromagnet 25, and immunomagnetic beads continue under the slow drive of first circulation motor 22 anti-
It answers in pipeline and circulates, by being attracted when the second 25 position of electromagnet, be gathered in 21 exit of reacting pipe, meanwhile,
One giant magnetoresistance chip module 23 measures the concentration of immunomagnetic beads in reacting pipe 21, when mean intensity value is lower than given threshold b,
It is believed that magnetic bead has been completely segregated, cleaning fluid pipe solenoid valve 131 is opened, the first waste solution channel solenoid valve 311 is opened, clear
Washing lotion pipeline 13 is passed through cleaning solution, and cleaning input duct 1, reacting pipe 21 and immunomagnetic beads, waste liquid is via the first waste solution channel
31 discharges.
The T4 time is waited, after the completion of cleaning, the second electromagnet 25 is closed, closes the first waste solution channel solenoid valve 311, open
The total solenoid valve 33 of reaction solution pipeline, sterile water enter input duct 1 via cleaning fluid pipe 13, and immunomagnetic beads is driven to flow to point
From device 4.
Separation process: controller controls several third electromagnet 41 according to certain timing on-off, passes through the rule of generation
It restrains electromagnetic field and drives mobile separation of the immunomagnetic beads in mixed solution, the second giant magnetoresistance chip module 45 detects immunomagnetic beads
Quantity.
For reaction solution in reaction solution pipeline 3, MCU unit controls each third electromagnet 41 according to certain timing individually or simultaneously
Magnetic field is generated, from left to right successively absorption immunomagnetic beads keep it mobile to 3 right end of reaction solution pipeline.
The first electromagnet 41 of left end first is powered, and the immunomagnetic beads in reaction solution pipeline 3 are adsorbed onto the first electromagnet
At pipeline among 24；It keeps first, left side third electromagnet 41 to be powered, opens the third electromagnet 41 of left end second, will exempt from
Epidemic disease magnetic bead is adsorbed onto the position among two magnet rings；The third electromagnet 41 of left end first is disconnected, second third electromagnet is kept
41 are powered, and immunomagnetic beads are adsorbed onto the position among second third electromagnet 41；Successively on-off residue electromagnet, until exempting from
Epidemic disease magnetic bead is moved to the position among the third electromagnet 41 of right end, opens the total solenoid valve 42 of separate pipeline, opens the 4th electricity
Magnet 44 opens the second waste solution channel solenoid valve 321, opens sterile water pipe electromagnetic valve 431 and injects sterile water, in the 4th electromagnetism
Under the collective effect of the vertical direction impact force of the horizontal direction attraction and sterile water of iron 44, immunomagnetic beads enter separation liquid pipe
Road 5, part sterile water will be redirected back into reaction solution pipeline 5 and be discharged from the second waste solution channel 32, complete the cleaning of immunomagnetic beads
And separation.
Second giant magnetoresistance chip module 45 acquires the signal of immunomagnetic beads quantity, is transmitted to MCU by signal processing module
Unit shows that most of magnetic bead has been collected when magnetic bead mean concentration is less than given threshold c in solution, opens separating liquid
Immunomagnetic beads are drained into detection device 6, close separating liquid pipe electromagnetic valve 51, continue to be passed through sterile water, beat by pipe electromagnetic valve 51
The second waste solution channel solenoid valve 321 is opened, the cleaning to each pipeline in separator 4 is completed.
Detection process: it drives immunomagnetic beads solution by separating liquid pipeline 5 using second circulation motor 61, uses the 5th electricity
Immunomagnetic beads are adsorbed on 64 surface of graphene sensor by magnet 65, detection are realized in electrochemical workstation, and will test letter
Number being transmitted to MCU unit by signal processing module is shown, participates in detection by 62 pairs of third giant magnetoresistance chip module
The quantity of immunomagnetic beads measures, thus the detection caused by the immunomagnetic beads being lost in pretreatment process in concentration calculation
Error compensates, and improves detection accuracy.
Detection control solenoid valve 66 is opened, second circulation motor 61 is opened and immunomagnetic beads solution is driven to lead in detection device 6,
Immunomagnetic beads are by the magnet magnetized rear generation electromagnetic field in third giant magnetoresistance chip module 62, so as to realize to magnetic bead quantity
Measurement, be transferred to accumulation calculating in MCU unit through signal processing module, open third electromagnet 41, exempting from for pipeline will be entered
Epidemic disease magnetic bead is adsorbed in graphene sensor 64.
When third giant magnetoresistance chip module 62 detects the average value of immunomagnetic beads concentration less than preset threshold d, show
All magnetic beads have all entered detection device 6, close detection control solenoid valve 66 and second circulation motor 61, save current time
Accumulated value d.
Start electrochemical workstation, electric signal is sent into MCU unit by amplification, the filtering of signal processing module.
After completing detection, open detection control solenoid valve 66 and second circulation motor 61 and to be passed through cleaning solution clear to pipeline
It washes.
The analyzer carries out precise quantification to immunomagnetic beads by using giant magnetoresistance chip module, reduces test error,
Effective test sample is remained to the greatest extent, improves the sensitivity of instrument, improves detection efficiency, and equipment arrives in the past
After multiple giant magnetoresistance chip modules are set, by the detection to different point immunomagnetic beads, to losing magnetic in reaction, separation process
The test error that pearl may cause is compensated, and keeps test data more accurate, improves the precision of equipment, reduce detection at
This, the device structure is compact, is conducive to a wide range of promote.
Reaction unit 2 by control of the MCU controller to each solenoid valve and each electromagnet, realize automation reaction and
Lock out operation, controller control each electromagnet timing on-off and cooperate first circulation motor 22, make immunomagnetic beads and sample solution
It comes into full contact with, reacts more thorough, by the way that electromagnet is arranged in 21 exit of annular reaction pipeline, while solution circulation flow is dynamic,
Keep the separation of immunomagnetic beads more thorough.
Separator 4 drives immunomagnetic beads to 3 end of reaction solution pipeline by carrying out the control of timing on-off to multiple electromagnet
End motion, compared with common liquid drives, separation is more thorough, after separating magnetic bead, carries out back flush to separate lines,
Pipeline-cleaning is more thorough, with it is traditional take liquid needle to design compared with, realize online separation, separating rate faster, improves point
From efficiency.
Detection device 6 is detected using graphene sensor 64, improves the sensitivity of detection device 6, molten in detection process
Liquid constant flow, real-time is good, realizes on-line checking.
The foregoing is merely the preferred embodiments of the invention, are not intended to limit the invention creation, all at this
Within the spirit and principle of innovation and creation, any modification, equivalent replacement, improvement and so on should be included in the invention
Protection scope within.
Claims (10)
1. a kind of electrochemical immunoanalytical instrument, it is characterised in that: including separator (4) and detection device (6), separator
It (4) include reaction solution pipeline (3), the sterile waterpipe (43) and separating liquid pipeline (5) that are connected to reaction solution pipeline (3), anti-
It answers and sets gradually several third electromagnet (41) on liquid pipe road (3), the separating liquid pipeline on the right side of right end third electromagnet (41)
(5) the total solenoid valve of separate pipeline (42) are set on, the prominent sterile waterpipe (43) in reaction solution pipeline (3) end forms protruding portion
(34), on protruding portion (34) be arranged the 4th electromagnet (44), by control the first electromagnet (41) on-off make immunomagnetic beads to
Reaction solution pipeline (3) end is mobile, and detection device (6) are arranged in separating liquid pipeline (5) end, and detection device (6) includes arriving in the past
It is successively set on second circulation motor (61) on separating liquid pipeline (5), third giant magnetoresistance chip module (62) afterwards, to electrode
(63), graphene sensor (64) are set on the tube wall opposite with the separating liquid pipeline (5) to electrode (63) is equipped with, graphene passes
5th electromagnet (65) is set on sensor (64).
2. electrochemical immunoanalytical instrument according to claim 1, it is characterised in that: reaction solution pipeline (3) front end with
Reaction unit (2) connection, reaction unit (2) include the reacting pipe (21) of annular, the liquid feeding end and feed liquor of reacting pipe (21)
Pipeline (1) connection, the outlet end of reacting pipe (21) are connected to reaction solution pipeline (3), the series connection access in reacting pipe (21)
First circulation motor (22) is arranged the first giant magnetoresistance chip module (23) on reacting pipe (21), with first circulation motor
(22) the first electromagnet (24) are set above the reacting pipe (21) of opposite side, close to the anti-of reacting pipe (21) export direction
It answers and the second electromagnet (25) is set on liquid pipe road (3).
3. electrochemical immunoanalytical instrument according to claim 2, it is characterised in that: the input duct (1) respectively with sample
Quality control road (11), immunomagnetic beads pipeline (12) are connected to cleaning fluid pipe (13).
4. electrochemical immunoanalytical instrument according to claim 1, it is characterised in that: be arranged on the reaction solution pipeline (3)
For the first waste solution channel (31) of cleaning solution waste liquid to be discharged, between sterile waterpipe (43) and the first waste solution channel (31)
Second waste solution channel (32) are set on reaction solution pipeline (3).
5. electrochemical immunoanalytical instrument according to claim 1-4, it is characterised in that: it further include controller, control
Device processed includes MCU unit, energy supply control module and signal processing module, and the electromagnetism of control pipeline on-off is respectively set on each pipeline
Valve, solenoid valve, electromagnet and circulation motor in all pipelines pass through energy supply control module respectively and are connected to MCU unit, own
Giant magnetoresistance chip module pass through signal processing module and be connected to MCU unit, MCU unit is connect with power module.
6. electrochemical immunoanalytical instrument according to claim 1, it is characterised in that: the third electromagnet (41) from a left side to
On reaction solution pipeline (3), third electromagnet (41) is electromagnetism ring or bare electromagnet or gradient electromagnetic for right uniform setting
Field mould group.
7. electrochemical immunoanalytical instrument according to claim 1, it is characterised in that: the 4th electromagnet (44) is electromagnetism
Ring, protruding portion (34) are blind pipe, and electromagnetism ring is set on blind pipe or the 4th electromagnet (44) is bare electromagnet, protruding portion
It (34) is flat tube, bare electromagnet is fixed in flat tube.
8. the analysis method of electrochemical immunoanalytical instrument, it is characterised in that: main including the following steps:
S1, MCU unit open sample pipe solenoid valve (111), immunomagnetic beads pipe electromagnetic valve (121) and the total electromagnetism of input duct
Valve (14) is opened first circulation motor (22), and sample solution and immunomagnetic beads solution are mixed into input duct (1), when immune
After magnetic bead solution and sample solution are enough, each solenoid valve is closed；
S2, MCU unit control the on-off of the first electromagnet (24), and first circulation motor (22) drives mixed solution in reacting pipe
(21) it is circulated in, after completion of the reaction, closes the first electromagnet (24), open the second electromagnet (25), immunomagnetic beads are by the
Two electromagnet (25) attract, and the first giant magnetoresistance chip module (23) measures the concentration of immunomagnetic beads in reacting pipe (21), when flat
When equal concentration value is lower than given threshold b, most of immunomagnetic beads are collected；
S3, the second electromagnet (25) are closed, opened the total solenoid valve of reaction solution pipeline (33), sterile water is logical from cleaning fluid pipe (13)
Enter and immunomagnetic beads is driven to flow into reaction solution pipeline (3), it is independent according to certain timing that MCU unit controls each third electromagnet (41)
Or magnetic field is generated simultaneously, from left to right successively absorption immunomagnetic beads keep it mobile to reaction solution pipeline (3) right end；
S4, MCU unit open the 4th electromagnet (44), open the total solenoid valve of separate pipeline (42), the second waste solution channel solenoid valve
(321) enter separating liquid pipeline (5) with sterile water pipe electromagnetic valve (431), immunomagnetic beads, part sterile water will be redirected back into instead
It answers liquid pipeline and is discharged from the second waste solution channel (32)；
The signal of S5, the second giant magnetoresistance chip module (45) acquisition immunomagnetic beads quantity, are transmitted to MCU by signal processing module
Unit is opened separating liquid pipe electromagnetic valve (51) when magnetic bead mean concentration is less than given threshold c in solution, contains immunomagnetic beads
Sterile water enter separating liquid pipeline (5), close separating liquid pipe electromagnetic valve (51)；
S6, detection control solenoid valve (66) is opened, opened second circulation motor (61), third giant magnetoresistance chip module (62) detection
Magnetic bead quantity is transferred to accumulation calculating in MCU unit through signal processing module, opens the 5th electromagnet (65), will enter pipeline
Immunomagnetic beads be adsorbed on graphene sensor (64), when third giant magnetoresistance chip module (62) detects immunomagnetic beads concentration
Average value when being less than preset threshold d, close detection control solenoid valve (66) and second circulation motor (61), preservation current time
Accumulated value d.
9. the analysis method of electrochemical immunoanalytical instrument according to claim 8, it is characterised in that: in the step S1,
Signal processing module acquires the signal of the first giant magnetoresistance chip module (23) detection immunomagnetic beads quantity, and signal processing module will be adopted
Collection signal is input to MCU unit and carries out accumulation process, when accumulated value reaches given threshold a, shows immunomagnetic beads solution foot
Amount closes sample pipe solenoid valve (111), immunomagnetic beads pipe electromagnetic valve (121) and the total solenoid valve of input duct (14).
10. the analysis method of electrochemical immunoanalytical instrument according to claim 8, it is characterised in that: in the step S3,
The third electromagnet (41) of left end first is powered, and the immunomagnetic beads in reaction solution pipeline (3) are adsorbed onto the third of left end first
At the intermediate pipeline of electromagnet (41), the third electromagnet (41) is kept to be powered, opens the third electromagnet (41) of left end second,
Immunomagnetic beads are adsorbed onto the position among two electromagnet, disconnect the third electromagnet (41) of left end first, are kept for second the
Three electromagnet (25) are powered, and immunomagnetic beads are adsorbed onto the intermediate position of second third electromagnet (41), successively on-off is remaining
Electromagnet, until immunomagnetic beads are moved to the position among the third electromagnet (41) of right end.
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