CN113030050B - Cell detection device with self-flushing structure and flushing method - Google Patents
Cell detection device with self-flushing structure and flushing method Download PDFInfo
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- CN113030050B CN113030050B CN202110270694.0A CN202110270694A CN113030050B CN 113030050 B CN113030050 B CN 113030050B CN 202110270694 A CN202110270694 A CN 202110270694A CN 113030050 B CN113030050 B CN 113030050B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
Abstract
The present invention provides a cell detection device having a self-flushing structure and a flushing method, comprising: the device comprises a magnetic enrichment module, a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample injection EP tube, a liquid storage bottle and a flushing unit; by adopting the structural design integrating the enrichment magnetic force and the fluorescence technology and combining a scientific counting method, the possible human errors caused by transferring other operation links in the middle are greatly reduced, and meanwhile, by adopting the design of a flushing unit, the detection technology of different samples to be detected can be continuously carried out for many times, so that the application range and the detection speed of the cell detection device are greatly improved; the integral recycling frequency of the equipment is improved, and the detection and counting cost is reduced.
Description
Technical Field
The invention belongs to the technical field of cell detection, and particularly relates to a cell detection device with a self-flushing structure and a flushing method.
Background
The concentration of cells or bacteria is an important physical parameter, and has strict and definite industry standard in the fields of food, medicine, immunodetection and the like.
Currently, detection methods of immune cell methods, flow cytometry, PCR quantitative detection or immunomagnetic bead enrichment combined with chemical light or fluorescence methods are more common; but the immune cell method has the advantages of small cell number detection and low efficiency; the sensitivity of flow cytometry detection is low; the PCR detection method has long time consumption, complex operation and low pollution level, and the existing detection method is difficult to accurately detect the sample, so that the enrichment treatment is required before the detection; in addition, some cells to be detected have non-culture property, and cannot be detected by adopting a traditional flat plate detection method;
the immunomagnetic beads have the advantages of good magnetic induction capability, superparamagnetism, high separability, high specificity, no influence on cell activity and the like, are gradually applied to cell concentration detection, the immunomagnetic beads are formed by coupling the surface activation of the magnetic beads with active proteins, and then the dye is utilized to penetrate the cell membrane of dead bacteria or damaged bacteria and combine with DNA thereof, so that the interference of other non-target microorganisms can be avoided, the detection accuracy is improved, but a plurality of separation devices are used in the current combined detection method, such as: firstly, a magnetic force device is used for enriching immune magnetic beads, then an enriched sample is taken out, and then a fluorescence imaging microscope or a fluorescence detection instrument is used for quantitative detection, so that the method has the advantages of more manual participation links, low efficiency and long time consumption.
Therefore, the cell quantitative detection device with high integration degree, low concentration and high concentration sample detection simultaneously, and high speed and high efficiency is one of the important problems how to ensure the repeated, circulating and uninterrupted use of the device and can give consideration to the pipeline cleaning and detection of the fluid fluorescent sorting device and the magnetic bead fluorescent sorting device.
Disclosure of Invention
In order to overcome the technical defects, the invention provides the cell detection device with the self-flushing structure and the flushing method, which adopt the structural design integrating the enrichment magnetic force, the fluid control and the fluorescence technology, and combine a scientific counting method, thereby greatly reducing the possible human errors caused by other operation links of intermediate transfer, and simultaneously adopting the flushing structural design, can continuously carry out the detection technology of different samples to be detected for many times, and greatly improve the application range and the detection speed of the cell detection device.
A cell detection device having a self-flushing structure and a flushing method, wherein:
a cell detection device having a self-flushing structure, comprising: the device comprises a magnetic enrichment module, a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample injection EP tube, a liquid storage bottle and a flushing unit;
as an illustration, the computer may also employ other processor units;
further, the magnetic force enrichment module is used for generating a magnetic force field, so that after a sample to be detected in the sample cavity is combined with the magnetic beads through a target object in the sample cavity, a side adsorption effect is generated under the action of the magnetic force field;
as an illustration, the magnetic force enrichment module is designed and manufactured by adopting an electromagnet structure or a permanent magnet structure;
further, the fluorescence excitation module is used for carrying out fluorescence excitation on the dyed target object and generating fluorescence;
further, the fluorescence detection imaging module is used for receiving the detected fluorescence and generating a fluorescence image;
further, the sample cavity is arranged in the magnetic force field of the magnetic force enrichment module and is used for containing a sample to be detected;
further, the displacement module is used for carrying the fluorescence detection imaging module and carrying out plane scanning on the cavity wall of the sample cavity;
as an illustration, the displacement module may also be used to carry the sample cavity, and perform a relative displacement of the fluorescence detection imaging module in a flat scanning operation to form a planar scanning effect;
further, the computer is used for carrying out algorithm processing on the fluorescence image so as to realize sample counting;
further, the fluid module is used for controlling the sample to be detected in the sample injection EP pipe to flow into the sample cavity, and is also used for controlling the waste liquid in the sample cavity to flow out, and the waste liquid is collected through the liquid storage bottle.
As an illustration, the fluid module performs fluid flow control by controlling the first, second, and third infusion pumps;
in actual use, the samples to be tested are various and are respectively stored in different sample injection EP pipes, the samples to be tested are transferred into the sample cavity from the different sample injection EP pipes one by one through the fluid module, so that the samples to be tested can remain in the PVC pipeline to cause pipeline pollution, and the detection of the samples to be tested next time can cause result deviation, and therefore, the invention also designs a flushing unit;
further, the flushing unit includes: the device comprises a first infusion pump, a second infusion pump, a third infusion pump, a flushing bottle and a three-way device;
further, one end of the first infusion pump is communicated with one end pipeline of the flushing bottle, the other end of the first infusion pump is communicated with a pipeline at one end of the three-way device, and the two ends of the three-way device are communicated with a pipeline of the sample injection EP pipe; the three-way end of the three-way device is communicated with the sample cavity pipeline; one end of the second infusion pump is communicated with the sample injection EP pipe; the other end of the second infusion pump is communicated with the sample cavity pipeline; one end of the third infusion pump is communicated with the sample cavity pipeline; the other end of the third infusion pump is communicated with the liquid storage bottle pipeline;
further, the flushing bottle is used for containing a cleaning solution;
as an example, a cell detection device with a self-flushing structure may further include: the device comprises a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample inlet tube, a liquid storage bottle and a flushing unit; the magnetic enrichment module is not needed, and the magnetic enrichment module is only used for fluorescent imaging observation without magnetic bead combination;
a washing method of a cell detection device with a self-washing structure, which is based on the washing after the cell detection method and the cell counting method are executed, comprises the following steps:
step one, controlling a magnetic force enrichment module to remove a magnetic force field around a sample cavity;
as an illustration, the magnetic force enrichment module of the electromagnetic structure can cancel the magnetic force field around the sample cavity by the mode of power off;
as an illustration, the magnetic force enrichment module of the permanent magnetic structure can remove the magnetic force field around the sample cavity by displacing the magnetic force enrichment module of the permanent magnetic structure;
as an illustration, when a cell detection device with a self-flushing structure for fluorescent imaging observation without magnetic bead binding is flushed, the flushing operation can be completed without controlling the operation of the magnetic force enrichment module, i.e. omitting the step one;
step two, a fluid module controls the first infusion pump, the second infusion pump and the third infusion pump to be started, the fluid module is provided with a flushing time, and when the flushing time is up, the fluid module controls the first infusion pump, the second infusion pump and the third infusion pump to be closed;
step three, the first infusion pump pumps the cleaning solution in the flushing bottle, and the cleaning solution is shunted into the sample injection EP pipe through the two-way end of the three-way device to clean the inside of the sample injection EP pipe;
step four, the second infusion pump pumps the cleaning solution in the sample injection EP pipe in real time, and the cleaning solution is transmitted to a sample cavity to clean the inside of the sample cavity;
when the high-concentration sample is washed and detected, the fluid module closes the second infusion pump because the sample injection EP pipe is not used for operation, and the washing solution is recovered through the liquid storage bottle after the sample cavity is directly washed through the first infusion pump, the third infusion pump and the three-way device;
step five, the third infusion pump pumps the cleaning solution in the sample cavity in real time, and transmits the cleaning solution to a liquid storage bottle for uniform recovery;
step six, after the flushing time is over, starting a fluorescence excitation module and a fluorescence detection imaging module, and repeating the step one to the step six when detecting that the fluorescence imaging exists between the sample injection EP tube and the sample cavity; when fluorescence imaging of the sample injection EP tube and the sample cavity is not detected, the cleaning is indicated, and the cleaning operation is finished;
as an illustration, the fluorescence imaging may be range-valued through a reasonable reserved interval of residual values, and there may be some residual value fluorescence imaging during the actual cleaning operation.
The invention has the beneficial effects that:
1. the invention has less manual participation links and high integration degree, and effectively avoids the problem of sample pollution caused by excessive manual participation links in the existing cell detection work;
2. through scientific structural design and combined with optimized detection and counting means, the defect of low cell detection and counting precision is effectively improved;
3. the invention adopts the design of an automatic cleaning structure, greatly improves the integral recycling frequency of the equipment and reduces the detection and counting cost.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a cell detection device with a self-washing structure according to the present invention
FIG. 2 is a graph showing the fluorescence effect of a target sample in a cell detection method according to the present invention
Detailed Description
The preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Referring to fig. 1 to 2, a cell detection apparatus with a self-washing structure and a washing method are provided, wherein:
a cell detection device having a self-flushing structure, comprising: the magnetic enrichment module 101, the fluorescence excitation module 102, the fluorescence detection imaging module 103, the sample cavity 104, the displacement module 105, the computer 106, the fluid module 107, the sample injection EP tube 108, the liquid storage bottle 109 and the flushing unit;
as an illustration, the computer 106 may employ other processor units as well;
further, the magnetic force enrichment module 101 is configured to generate a magnetic force field, so that after a sample to be detected in the sample cavity 104 is combined with the magnetic beads through a target object therein, a side adsorption effect is generated under the action of the magnetic force field;
as an illustration, the magnetic force enrichment module 101 is made by adopting an electromagnet structural design;
further, the fluorescence excitation module 102 is configured to perform fluorescence excitation on the dyed target object and generate fluorescence;
further, the fluorescence detection imaging module 103 is configured to receive the detected fluorescence and generate a fluorescence image;
further, the sample cavity 104 is disposed in the magnetic force field of the magnetic force enrichment module, and is used for holding a sample to be measured;
further, the displacement module 105 is configured to carry the fluorescence detection imaging module 103, and perform a planar scan on a cavity wall of the sample cavity 104;
as an illustration, the displacement module 105 may also be used to carry the sample cavity 104, and perform a relative displacement of the fluorescence detection imaging module 103 in a flat scanning operation to form a planar scanning effect;
further, the computer 106 is configured to perform an algorithm on the fluorescence image, so as to implement sample counting;
further, the fluid module 107 is configured to control the sample to be tested in the sample injection EP tube 108 to flow into the sample cavity 104, and is further configured to control the waste liquid in the sample cavity 104 to flow out, and collect the waste liquid through the liquid storage bottle 109.
As an illustration, the fluid module 107 performs fluid flow control by controlling the first infusion pump 111, the second infusion pump 112, and the third infusion pump 113;
in actual use, the samples to be tested are various and are respectively stored in different sample injection EP pipes, and the samples to be tested are transferred into the sample cavity from different sample injection EP pipes one by one through the fluid module 107, so that the samples to be tested can remain in the PVC pipeline to cause pipeline pollution, and the detection of the samples to be tested next time can cause result deviation, therefore, the invention also designs a flushing unit;
further, the flushing unit includes: a first infusion pump 111, a second infusion pump 112, a third infusion pump 113, a infusion bottle 110, and a three-way device 114;
further, one end of the first infusion pump 111 is in pipeline communication with one end of the infusion bottle 110, the other end of the first infusion pump 111 is in pipeline communication with one end of the three-way device 114, and the two ends of the three-way device 114 are in pipeline communication with the sample injection EP pipe 108; the three-way end of the three-way device 114 is in pipeline communication with the sample cavity 104; one end of the second infusion pump 112 is in pipeline communication with the sample injection EP tube 108; the other end of the second infusion pump 112 is in pipeline communication with the sample cavity 104; one end of the third infusion pump 113 is in pipeline communication with the sample cavity 104; the other end of the third infusion pump 113 is in pipeline communication with the liquid storage bottle 109;
further, the rinse bottle 110 is used for containing a cleaning solution;
as an example, a cell detection device with a self-flushing structure may further include: the device comprises a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample inlet tube, a liquid storage bottle and a flushing unit, wherein the replenishment schematic diagram is shown; the magnetic enrichment module is not needed, and the magnetic enrichment module is only used for fluorescent imaging observation without magnetic bead combination;
a washing method of a cell detection device with a self-washing structure, which is based on the washing after the cell detection method and the cell counting method are executed, comprises the following steps:
step one, controlling a magnetic force enrichment module 101 to remove a magnetic force field around a sample cavity 104;
as an illustration, the magnetic force enrichment module of the electromagnetic structure can cancel the magnetic force field around the sample cavity by the mode of power off;
as an illustration, the magnetic force enrichment module of the permanent magnetic structure can remove the magnetic force field around the sample cavity by displacing the magnetic force enrichment module of the permanent magnetic structure;
as an illustration, when a cell detection device with a self-flushing structure for fluorescent imaging observation without magnetic bead binding is flushed, the flushing operation can be completed without controlling the operation of the magnetic force enrichment module, i.e. omitting the step one;
step two, the fluid module 107 controls the first infusion pump 111, the second infusion pump 112 and the third infusion pump 113 to be turned on, the fluid module 107 is provided with a flushing time, and when the flushing time is up, the fluid module 107 controls the first infusion pump 111, the second infusion pump 112 and the third infusion pump 113 to be turned off;
step three, the first infusion pump 111 pumps the cleaning solution in the flushing bottle 110, and shunts the cleaning solution into the sample injection EP tube 108 through the two ends of the three-way device 114, so as to clean the inside of the sample injection EP tube 108;
step four, the second infusion pump 112 extracts the cleaning solution in the sample injection EP tube 108 in real time, and transmits the cleaning solution to the sample cavity 104, so as to clean the interior of the sample cavity 104;
when the sample is washed and detected, the fluid module 107 closes the second infusion pump 112 due to the operation of not using the sample injection EP pipe, directly washes the sample cavity 104 through the first infusion pump 111, the third infusion pump 113 and the three-way device 114, and then recovers the washing solution through the liquid storage bottle 109;
step five, the third infusion pump 113 extracts the cleaning solution in the sample cavity 104 in real time, and transmits the cleaning solution to the liquid storage bottle 109, so as to uniformly recycle the cleaning solution;
step six, after the flushing time is over, starting the fluorescence excitation module 102 and the fluorescence detection imaging module 103, and repeating the steps one to five when detecting that the fluorescence imaging exists between the sample injection EP tube 108 and the sample cavity 104; when fluorescence imaging of the sample introduction EP tube 108 and the sample cavity 104 is not detected, the cleaning is indicated, and the cleaning operation is finished;
as an illustration, the fluorescence imaging may be range-valued through a reasonable reserved interval of residual values, and there may be some residual value fluorescence imaging during the actual cleaning operation.
In order to better explain the working principle of the cell detection device with the self-flushing structure, the following description is presented by the cooperation of detection and counting operations:
a method of cell detection comprising: a high-concentration sample detection method and a low-concentration sample detection method;
the method for detecting the high-concentration sample to be detected comprises the following steps:
step one, after a sample to be detected is pretreated by the front end, combining a target object in the sample to be detected with magnetic beads;
as an illustration, the concentration of the magnetic beads should be greater than the concentration of the target in the sample to be tested;
step two, performing fluorescent staining treatment on the sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads;
step three, injecting a quantitative sample to be detected into the sample cavity 104, and placing the sample cavity 104 into the magnetic field range of the magnetic enrichment module 101; at this time, the magnetic beads combined with the target and the redundant magnetic beads not combined with the target are adsorbed to the cavity wall at one side of the sample cavity 104 close to the magnetic force field;
as an illustration, the magnetic force enrichment module 101 adopts an electromagnet structure, and generates a magnetic force field after being electrified; the magnetic force field disappears after the power is off, and compared with a magnetic force enrichment module of a permanent magnetic structure, the effect of the electromagnetic structure is better;
step four, because the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescence interference; therefore, after the magnetic enrichment module 101 is in effect, the fluidic module 107 is opened, and the waste liquid in the sample cavity 104 is discharged to the liquid storage bottle 109;
step five, starting the fluorescence excitation module 102 to enable the target on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample;
and step six, receiving the fluorescence by adopting a fluorescence detection imaging module 103 to generate a fluorescence image, and transmitting the fluorescence image to the computer 106 for graphic processing and counting display.
The method has simple and convenient process operation, large image scanning area and is more suitable for high-concentration samples.
The method for detecting the low-concentration sample to be detected comprises the following steps:
step one, after a sample to be detected is pretreated by the front end, combining a target object in the sample to be detected with magnetic beads;
as an illustration, the concentration of the magnetic beads should be greater than the concentration of the target in the sample to be tested;
step two, performing fluorescent staining treatment on the sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads;
step three, sealing a quantitative sample to be measured in a sample injection EP pipe 108, wherein the sample to be measured is controlled by the fluid module 107 and sequentially transferred into a sample cavity 104 from the sample injection EP pipe until all the samples to be measured are transferred; at this time, the magnetic beads combined with the target and the redundant magnetic beads not combined with the target are sequentially adsorbed on the cavity wall at one side of the sample cavity 104 close to the magnetic force field;
step four, because the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescence interference; therefore, the residual waste liquid of the sample to be measured in the sample cavity 104 is completely discharged into the liquid storage bottle 109 through the fluid module 107;
step five, starting the fluorescence excitation module 102 to enable the target on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample;
and step six, receiving the fluorescence by adopting a fluorescence detection imaging module 107, generating a fluorescence image, and transmitting the fluorescence image to the computer 106 for graphic processing and counting display.
The method has small scanning area, and is more suitable for low-concentration samples through the enrichment process.
As an illustration, the sample-in EP tube 108 may be replaced with other sealing architectures;
a cell counting method, which carries out algorithm processing on fluorescent images through a computer 106 so as to realize sample counting; the method comprises the following specific steps:
step one, the fluorescence imaging effect is shown in figure 2; the coverage area of the fluorescent image on the cavity wall is often larger than one imaging field of view, and the cavity wall of the sample cavity 104 needs to be subjected to planar scanning to complete counting statistics;
step two, realizing the plane scanning action of the cavity wall through the displacement module 105; the plane scanning action of the displacement module 105 is calculated as follows:
firstly, setting the imaging size width of a single fluorescent image as W and the height as H;
next, when the displacement module 105 moves to the upper left scanning edge of the sample chamber 104, the motion system coordinates are recorded as (X 0 ,Y 0 ) When the displacement module 105 moves to the upper right scanning edge of the sample chamber 104, the motion system coordinate is recorded as (X 1 ，Y 1 ) When the displacement module 105 moves to the lower left scanning edge of the sample chamber 104, the motion system coordinate is recorded as (X 2 ，Y 2 ) The method comprises the steps of carrying out a first treatment on the surface of the From the left edge to the right edge of the sample chamber 104, the displacement module 105 needs to be calculatedMaximum number of shifts n= (X) 1 -X 0 ) Moving from the upper edge to the lower edge of the sample chamber 104, the displacement module 105 needs to be moved a maximum number of times m= (Y) 2 -Y 0 )/H；
Finally, because the scanning area of the sample cavity 104 is large, in order to ensure that the target sample can be imaged clearly in the full scanning stroke, Z-direction focusing is needed to obtain high-quality imaging, so that fluorescence counting is accurate, and because the concentration content of the target sample is uncertain, certain imaging areas have no fluorescence at all, and counting operation cannot be completed by using the traditional automatic focusing, the invention adopts a positioning compensation method to perform diagonal compensation;
the positioning compensation method comprises the following steps:
(1) is arranged at (X) 0 ,Y 0 ) The position, the Z direction position of clear imaging is Z 0 The method comprises the steps of carrying out a first treatment on the surface of the In (X) 1 ，Y 1 ) The position, Z-direction clear position is Z X The method comprises the steps of carrying out a first treatment on the surface of the In (X) 2 ，Y 2 ) The clear position of the position imaging is Z y ；
(2) When the displacement module 105 scans the ith row (1.ltoreq.i.ltoreq.N) and the jth column (1.ltoreq.j.ltoreq.M) of the sample cavity 104, the coordinates of each motion axis are:
X＝X 0 +i*W；
Y＝Y 0 +j*H；
Z＝Z 0 +i*(Z x -Z 0 )/N+j*(Z y -Z 0 )/M；
after compensation calculation, clear scanning imaging of the whole chamber range can be realized;
step three, after the displacement module 105 scans a certain position of the cavity wall of the sample cavity 104, the state of the sample on the current cavity wall is transmitted into the computer 106 through the fluorescence detection imaging module 103, and the fluorescence target is extracted through an image processing algorithm; accumulating the fluorescence identification results of each scanning surface to obtain a current counting result;
as an illustration, the position of the sample chamber 104 is not limited to being flat, but may be vertical or at other angles; the magnetic enrichment module 101, the fluorescence excitation module 102 and the fluorescence detection imaging module 103 are consistent with the angular positions of the sample cavity 104, so that the magnetic enrichment module 101 and the fluorescence excitation module 102 are ensured to be effective, and the fluorescence detection imaging module 103 can accurately image;
for a better illustration of the principles of the present invention, the following are set forth by way of examples: example 1:
high concentration sample detection method data:
after the front end pretreatment of the sample to be tested, combining the target in the sample to be tested with the magnetic beads; the concentration of the magnetic beads is larger than that of the target substances in the sample to be detected; carrying out fluorescent staining treatment on a sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads; 1ml of a sample to be detected is injected into the sample cavity 104, the volume of the sample cavity is 1.5ml, and the sample cavity 104 is placed in the magnetic field range of the magnetic enrichment module 101; at this time, the magnetic beads combined with the target and the redundant magnetic beads not combined with the target are adsorbed to the cavity wall at one side of the sample cavity 104 close to the magnetic force field; the magnetic force enrichment module 101 adopts an electromagnet structure, and generates a magnetic force field after being electrified; after the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescent interference; therefore, after the magnetic enrichment module 101 is in effect, the fluidic module 107 is opened, and the waste liquid in the sample cavity 104 is discharged to the liquid storage bottle 109; starting the fluorescence excitation module 102 to enable the target on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample; the fluorescence detection imaging module 103 is used for receiving the fluorescence generated fluorescence image, and the fluorescence image is transmitted to the computer 106 for graphic processing and counting display.
Example 2
Low concentration test sample detection
After the front end pretreatment of the sample to be tested, combining the target in the sample to be tested with the magnetic beads; the concentration of the magnetic beads is larger than that of the target substances in the sample to be detected; carrying out fluorescent staining treatment on a sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads; sealing 1ml of sample to be tested in a sample injection EP pipe 108, wherein the sample to be tested is controlled by the fluid module 107 and sequentially transferred into a sample cavity 104 from the sample injection EP pipe, and the sample cavity 104 adopts a volume of 1.5ml until all the samples to be tested are transferred; at this time, the magnetic beads combined with the target and the redundant magnetic beads not combined with the target are sequentially adsorbed on the cavity wall at one side of the sample cavity 104 close to the magnetic force field; after the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescent interference; therefore, the residual waste liquid of the sample to be measured in the sample cavity 104 is completely discharged into the liquid storage bottle 109 through the fluid module 107; starting the fluorescence excitation module 102 to enable the target on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample; receiving the fluorescence by adopting a fluorescence detection imaging module 107, generating a fluorescence image, and transmitting the fluorescence image to the computer 106 for graphic processing and counting display; the method is more suitable for low-concentration samples through the enrichment process.
The foregoing description of the preferred embodiments of the present invention has been presented only to facilitate the understanding of the principles of the invention and its core concepts, and is not intended to limit the scope of the invention in any way, however, any modifications, equivalents, etc. which fall within the spirit and principles of the invention should be construed as being included in the scope of the invention.
Claims (10)
1. A cell detection device having a self-flushing structure, comprising: the device comprises a magnetic enrichment module, a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample injection EP tube, a liquid storage bottle and a flushing unit;
the magnetic force enrichment module is used for generating a magnetic force field, so that a sample to be detected in the sample cavity is combined with the magnetic beads through a target object in the sample cavity, and then a side adsorption effect is generated under the action of the magnetic force field; the fluorescence excitation module is used for carrying out fluorescence excitation on the dyed target object and generating fluorescence; the fluorescence detection imaging module is used for receiving the detected fluorescence and generating a fluorescence image; the sample cavity is arranged in the magnetic force field of the magnetic force enrichment module and is used for containing a sample to be detected; the displacement module is used for carrying the fluorescence detection imaging module and carrying out plane scanning on the cavity wall of the sample cavity; the computer is used for carrying out algorithm processing on the fluorescent image so as to realize sample counting; the fluid module is used for controlling a sample to be detected in the sample injection EP pipe to flow into the sample cavity and controlling waste liquid in the sample cavity to flow out, and collecting the waste liquid through the liquid storage bottle; the fluid module performs liquid flow control by controlling the first infusion pump, the second infusion pump and the third infusion pump;
the flush unit includes: the device comprises a first infusion pump, a second infusion pump, a third infusion pump, a flushing bottle and a three-way device; one end of the first infusion pump is communicated with one end pipeline of the flushing bottle, the other end of the first infusion pump is communicated with a pipeline at one end of the three-way device, and the two ends of the three-way device are communicated with a pipeline of the sample injection EP pipe; the three-way end of the three-way device is communicated with the sample cavity pipeline; one end of the second infusion pump is communicated with the sample injection EP pipe; the other end of the second infusion pump is communicated with the sample cavity pipeline; one end of the third infusion pump is communicated with the sample cavity pipeline; the other end of the third infusion pump is communicated with the liquid storage bottle pipeline; the flushing bottle is used for containing the cleaning solution.
2. The device of claim 1, wherein the computer is further configured to employ a processor unit.
3. The device of claim 1, wherein the magnetic enrichment module is an electromagnet structure.
4. The device of claim 1, wherein the displacement module is configured to carry the sample chamber and perform a flat scanning operation on the fluorescence detection imaging module to form a flat scanning effect.
5. A cell detection device having a self-flushing structure, comprising: the device comprises a fluorescence excitation module, a fluorescence detection imaging module, a sample cavity, a displacement module, a computer, a fluid module, a sample inlet tube, a liquid storage bottle and a flushing unit; removing a magnetic enrichment module for fluorescent imaging observation without magnetic bead combination; the flush unit includes: the device comprises a first infusion pump, a second infusion pump, a third infusion pump, a flushing bottle and a three-way device; one end of the first infusion pump is communicated with one end pipeline of the flushing bottle, the other end of the first infusion pump is communicated with a pipeline at one end of the three-way device, and the two ends of the three-way device are communicated with a pipeline of the sample injection EP pipe; the three-way end of the three-way device is communicated with the sample cavity pipeline; one end of the second infusion pump is communicated with the sample injection EP pipe; the other end of the second infusion pump is communicated with the sample cavity pipeline; one end of the third infusion pump is communicated with the sample cavity pipeline; the other end of the third infusion pump is communicated with the liquid storage bottle pipeline; the flushing bottle is used for containing the cleaning solution.
6. A washing method of a cell detection device having a self-washing structure, the method comprising the steps of:
step one, controlling a magnetic force enrichment module to remove a magnetic force field around a sample cavity;
step two, a fluid module controls the first infusion pump, the second infusion pump and the third infusion pump to be started, the fluid module is provided with a flushing time, and when the flushing time is up, the fluid module controls the first infusion pump, the second infusion pump and the third infusion pump to be closed;
step three, the first infusion pump pumps the cleaning solution in the flushing bottle, and the cleaning solution is shunted into the sample injection EP pipe through the two-way end of the three-way device to clean the inside of the sample injection EP pipe;
step four, the second infusion pump pumps the cleaning solution in the sample injection EP pipe in real time, and the cleaning solution is transmitted to a sample cavity to clean the inside of the sample cavity;
when the high-concentration sample is washed and detected, the fluid module closes the second infusion pump because the sample injection EP pipe is not used for operation, and the washing solution is recovered through the liquid storage bottle after the sample cavity is directly washed through the first infusion pump, the third infusion pump and the three-way device;
step five, the third infusion pump pumps the cleaning solution in the sample cavity in real time, and transmits the cleaning solution to a liquid storage bottle for uniform recovery;
step six, after the flushing time is finished, starting a fluorescence excitation module and a fluorescence detection imaging module, repeating the step one to the step five when detecting that the fluorescence imaging exists between the sample injection EP tube and the sample cavity, and indicating that the sample injection EP tube and the sample cavity are cleaned when the fluorescence imaging does not exist between the sample injection EP tube and the sample cavity, and finishing the cleaning operation;
the cell detection method comprises the following steps: a high-concentration sample detection method and a low-concentration sample detection method;
the method for detecting the high-concentration sample to be detected comprises the following steps:
step one, after a sample to be detected is pretreated by the front end, combining a target object in the sample to be detected with magnetic beads;
step two, performing fluorescent staining treatment on the sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads;
step three, injecting a quantitative sample to be detected into a sample cavity, and placing the sample cavity into the magnetic field range of the magnetic enrichment module; at this time, the magnetic beads combined with the target object and the redundant magnetic beads not combined with the target object are adsorbed on the cavity wall at one side of the sample cavity close to the magnetic force field;
step four, because the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescence interference; therefore, after the magnetic enrichment module is effective, the fluid module is started, and the waste liquid in the sample cavity is discharged to the liquid storage bottle;
step five, starting a fluorescence excitation module to enable a target object on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample;
step six, adopting a fluorescence detection imaging module to receive the fluorescence to generate a fluorescence image, and transmitting the fluorescence image to the computer for graphic processing and counting display;
the method for detecting the low-concentration sample to be detected comprises the following steps:
step one, after a sample to be detected is pretreated by the front end, combining a target object in the sample to be detected with magnetic beads;
step two, performing fluorescent staining treatment on the sample to be detected, so that the target object can exhibit fluorescence after being combined with the magnetic beads;
sealing a quantitative sample to be measured in a sample injection EP tube, wherein the sample to be measured is controlled by the fluid module and sequentially transferred into a sample cavity from the sample injection EP tube until all the samples to be measured are transferred; at the moment, the magnetic beads combined with the target object and the redundant magnetic beads not combined with the target object are sequentially adsorbed on the cavity wall at one side, close to the magnetic force field, of the sample cavity;
step four, because the sample to be measured is dyed, more fluorescent impurities are generated in the sample to be measured, and the fluorescent impurities generate fluorescence interference; so, the residual waste liquid of the sample to be detected in the sample cavity is completely discharged into the liquid storage bottle through the fluid module;
step five, starting a fluorescence excitation module to enable a target object on the cavity wall to generate fluorescence; at this time, the sample which is adsorbed on the cavity wall and exhibits fluorescence is the target sample;
step six, adopting a fluorescence detection imaging module to receive the fluorescence, generating a fluorescence image, and transmitting the fluorescence image to the computer for pattern processing and counting display;
the cell counting method is that the computer carries out algorithm processing on the fluorescent image, thereby realizing sample counting; comprising the following steps:
step one, the coverage area of a fluorescent image on the cavity wall is always larger than one imaging visual field, and the cavity wall of a sample cavity needs to be subjected to plane scanning to complete counting statistics;
secondly, realizing the plane scanning action of the cavity wall through the displacement module; the plane scanning action of the displacement module is calculated as follows:
firstly, setting the imaging size width of a single fluorescent image as W and the height as H;
next, when the displacement module moves to the upper left scanning edge of the sample chamber, the motion system coordinate is recorded as (X 0 ,Y 0 ) When the displacement module moves to the upper right scanning edge of the sample cavity, the coordinate of the movement system is recorded as (X 1 ，Y 1 ) When the displacement module moves to the left lower scanning edge of the sample cavity, the coordinate of the movement system is recorded as (X 2 ，Y 2 ) The method comprises the steps of carrying out a first treatment on the surface of the From the left edge to the right edge of the sample cavity, the displacement module needs to be moved a maximum number of times n= (X) 1 -X 0 ) Moving from the upper edge to the lower edge of the sample chamber, the displacement module needs to be moved a maximum number of times m= (Y) 2 -Y 0 )/H；
Finally, because the scanning area of the sample cavity is large, in order to ensure that the target sample can be imaged clearly in the full scanning stroke, Z-direction focusing is needed to obtain high-quality imaging, so that the fluorescence counting is accurate, and because the concentration content of the target sample is uncertain, certain imaging areas have no fluorescence at all and can not finish the counting operation by using the traditional automatic focusing, the diagonal compensation is performed by adopting a positioning compensation method;
the positioning compensation method comprises the following steps:
(1) is arranged at (X) 0 ,Y 0 ) The position, the Z direction position of clear imaging is Z 0 The method comprises the steps of carrying out a first treatment on the surface of the In (X) 1 ，Y 1 ) The position, Z-direction clear position is Z X The method comprises the steps of carrying out a first treatment on the surface of the In (X) 2 ，Y 2 ) The clear position of the position imaging is Z y ；
(2) When the displacement module scans the ith row (i is less than or equal to 1 and is less than or equal to N) and the jth column (j is less than or equal to 1 and is less than or equal to M) of the sample cavity, the coordinates of each motion axis are as follows:
X＝X 0 +i*W；
Y＝Y 0 +j*H；
Z＝Z 0 +i*(Z x -Z 0 )/N+j*(Z y -Z 0 )/M；
after compensation calculation, clear scanning imaging of the whole chamber range can be realized;
step three, after the displacement module scans a certain position of the cavity wall of the sample cavity, the state of the sample on the current cavity wall is transmitted into a computer through the fluorescence detection imaging module, and fluorescence target extraction is carried out through an image processing algorithm; and accumulating the fluorescence identification results of all the scanning surfaces to obtain the current counting result.
7. The method according to claim 6, wherein the fluorescence imaging is performed by setting a range value in a reasonable retention range of the residual value.
8. The method according to claim 6, wherein the magnetic force enrichment module of the electromagnetic structure can remove the magnetic force field around the sample chamber in a power-off mode.
9. The method according to claim 6, wherein the magnetic force field around the sample chamber is removed by displacing the magnetic force enrichment module of the permanent magnet structure.
10. The method according to claim 6, wherein the washing operation is performed without controlling the operation of the magnetic force enrichment module when washing the cell detection device for fluorescent imaging observation without magnetic bead binding, and without omitting the step one.
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06300760A (en) * | 1993-04-15 | 1994-10-28 | Matsushita Electric Ind Co Ltd | Automatic fluorescence detector |
| WO1995031481A1 (en) * | 1994-05-18 | 1995-11-23 | The Research And Development Institute, Inc. | Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms |
| CN101672841A (en) * | 2008-09-09 | 2010-03-17 | 北京朔望科技有限公司 | Detection instrument and detection method for biological sample |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| CN101773861A (en) * | 2010-04-02 | 2010-07-14 | 华中科技大学 | Microfluidic sample feeding method, device and application thereof |
| WO2011105507A1 (en) * | 2010-02-24 | 2011-09-01 | 財団法人神奈川科学技術アカデミー | Cell analyzer |
| CN202049159U (en) * | 2011-04-11 | 2011-11-23 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterium sorting and labeling device based on immune method |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| CN103901189A (en) * | 2014-04-16 | 2014-07-02 | 北京化工大学 | Automatic immunodetection micro-fluidic chip fluorescent quantification detection device |
| WO2014120956A1 (en) * | 2013-01-30 | 2014-08-07 | Asta Fluidic Technologies, Inc. | Microfluidic-based fetal red blood cell detection |
| CN105738637A (en) * | 2016-02-01 | 2016-07-06 | 北京理工大学 | Full-automatic capillary electrophoresis device suitable for separation analysis of space biological sample |
| CN108279230A (en) * | 2018-02-02 | 2018-07-13 | 哈尔滨工业大学 | A kind of micro-fluidic type water quality toxicity analysis and detection device and its determination method |
| CN110456076A (en) * | 2019-09-12 | 2019-11-15 | 太仓极光克赛生物技术有限公司 | The method of multiple antibody immune magnetic beads enrichment detection CTCs |
| WO2020133635A1 (en) * | 2018-12-26 | 2020-07-02 | 深圳天依生命健康科技有限公司 | Fully automatic sperm cell detector |
-
2021
- 2021-03-13 CN CN202110270694.0A patent/CN113030050B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06300760A (en) * | 1993-04-15 | 1994-10-28 | Matsushita Electric Ind Co Ltd | Automatic fluorescence detector |
| WO1995031481A1 (en) * | 1994-05-18 | 1995-11-23 | The Research And Development Institute, Inc. | Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms |
| CN101672841A (en) * | 2008-09-09 | 2010-03-17 | 北京朔望科技有限公司 | Detection instrument and detection method for biological sample |
| CN101750450A (en) * | 2008-12-17 | 2010-06-23 | 中国科学院大连化学物理研究所 | Automatic sampling device for array capillary electrophoresis |
| WO2011105507A1 (en) * | 2010-02-24 | 2011-09-01 | 財団法人神奈川科学技術アカデミー | Cell analyzer |
| CN101773861A (en) * | 2010-04-02 | 2010-07-14 | 华中科技大学 | Microfluidic sample feeding method, device and application thereof |
| CN202049159U (en) * | 2011-04-11 | 2011-11-23 | 中国人民解放军军事医学科学院卫生装备研究所 | Automatic bacterium sorting and labeling device based on immune method |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| WO2014120956A1 (en) * | 2013-01-30 | 2014-08-07 | Asta Fluidic Technologies, Inc. | Microfluidic-based fetal red blood cell detection |
| CN103901189A (en) * | 2014-04-16 | 2014-07-02 | 北京化工大学 | Automatic immunodetection micro-fluidic chip fluorescent quantification detection device |
| CN105738637A (en) * | 2016-02-01 | 2016-07-06 | 北京理工大学 | Full-automatic capillary electrophoresis device suitable for separation analysis of space biological sample |
| CN108279230A (en) * | 2018-02-02 | 2018-07-13 | 哈尔滨工业大学 | A kind of micro-fluidic type water quality toxicity analysis and detection device and its determination method |
| WO2020133635A1 (en) * | 2018-12-26 | 2020-07-02 | 深圳天依生命健康科技有限公司 | Fully automatic sperm cell detector |
| CN110456076A (en) * | 2019-09-12 | 2019-11-15 | 太仓极光克赛生物技术有限公司 | The method of multiple antibody immune magnetic beads enrichment detection CTCs |
Non-Patent Citations (5)
| Title |
|---|
| 光纤嵌入式电泳芯片激光诱导荧光检测系统;杨飞;杨晓博;闫卫平;;仪表技术与传感器(第05期);全文 * |
| 全自动顺序注射在线四道样品/试剂引入装置的研制及应用;江林;田芹;裴晓华;王建华;;现代科学仪器(第04期);全文 * |
| 固相细胞计数法――一种快速检测单细胞细菌的食品微生物新检测方法;王晓英;国外医学.卫生学分册(第03期);全文 * |
| 基于磁分选技术和间接免疫荧光技术的肺癌A549细胞的分离检测;周朝辉;付聪颖;贾能勤;;上海师范大学学报(自然科学版)(第04期);全文 * |
| 微流控免疫芯片富集/捕获肠出血性大肠杆菌O157∶H7的研究;管潇;张惠静;毕颖楠;张莉;郝敦玲;韦劲宇;;第三军医大学学报(第10期);全文 * |
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