CN108828032A - A kind of online electrochemical detection device, method and electrochemical immunoanalytical instrument - Google Patents
A kind of online electrochemical detection device, method and electrochemical immunoanalytical instrument Download PDFInfo
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- 238000000835 electrochemical detection Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title abstract description 17
- 239000011324 bead Substances 0.000 claims abstract description 68
- 238000001514 detection method Methods 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 47
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 19
- 239000002699 waste material Substances 0.000 claims description 23
- 238000004140 cleaning Methods 0.000 claims description 18
- 238000012545 processing Methods 0.000 claims description 16
- 239000008223 sterile water Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 5
- 230000005518 electrochemistry Effects 0.000 claims description 4
- 238000009825 accumulation Methods 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 abstract description 35
- 238000012360 testing method Methods 0.000 abstract description 13
- 238000000926 separation method Methods 0.000 abstract description 12
- 230000035945 sensitivity Effects 0.000 abstract description 7
- 239000000243 solution Substances 0.000 description 56
- 239000000523 sample Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 230000005672 electromagnetic field Effects 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003447 ipsilateral effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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Abstract
The invention provides a kind of online electrochemical detection device, method and electrochemical immunoanalytical instrument, including separating liquid pipeline, second circulation motor, third giant magnetoresistance chip module are set gradually on separating liquid pipeline from front to back and to electrode, graphene sensor is set on the tube wall opposite with the separating liquid pipeline to electrode is equipped with, the 5th electromagnet is set in graphene sensor.Detection device described in the invention is detected using graphene sensor, improve the sensitivity of detection device, immunomagnetic beads are precisely quantified using giant magnetoresistance chip module, the test error that loss magnetic bead may cause in reaction, separation process is compensated, keep test data more accurate, the precision of equipment is improved, testing cost is reduced.
Description
Technical field
The invention belongs to online technical field of electrochemical detection, fills more particularly, to a kind of online Electrochemical Detection
It sets, method and electrochemical immunoanalytical instrument.
Background technique
The concentration of antigen/antibody in biofluid and its health status, physiological function, physiological period etc. have direct pass
System, so the concentration of quantitative detection antigen/antibody is significant to the mankind.The antigen/antibody in biofluid detects skill at present
Art mainly has：ELISA technology, chemiluminescence immunoassay technology, immunochromatography technique etc., but these methods have
Respective advantage and defect.
As the detection technique of a new generation, electrochemical immunoanalytical determination techniques have high sensitivity, selective good, detection
The features such as quick, shows wide application prospect in fields such as biomedicine, environmental monitoring, food inspections.In recent years
Come, the research work of electrochemical detection method achieves huge progress, and performance and type have also obtained quick development.So
And current instrument generallys use shifting sample, the mode of cleaning cooperates electrochemical workstation to be detected, due to depositing for liquid channel system
Cost, the volume of equipment greatly increase, and detection time is relatively long, and a possibility that cross contamination is also larger between different samples.
Demand to quick, sensitive electrochemical detection method is increasingly increasing, and on-line checking is one of very good solution side
Case.
Online electrochemical detection method mainly has following advantage compared with other technologies：1) it without pipetting, avoids and exempts from
Epidemic disease magnetic bead is largely lost, and improves detection sensitivity；2) good compatibility, high degree of automation；3) detection speed is fast；4) model is applied
It encloses wide；5) error caused by manual operation factor is reduced, however, it is still not perfect for the device of online Electrochemical Detection, need
Design a kind of device of higher online Electrochemical Detection of detection sensitivity.
Summary of the invention
In view of this, the invention is directed to a kind of online electrochemical detection device, method and electro-chemistry immunity point
Analyzer, to solve the above technical problems.
In order to achieve the above objectives, the technical solution of the invention is realized in：
A kind of online electrochemical detection device, including separating liquid pipeline set gradually on separating liquid pipeline from front to back
Two circulation motors, third giant magnetoresistance chip module and to electrode, and are equipped with the separating liquid pipeline opposite to electrode middle position
Graphene sensor is set on tube wall, the 5th electromagnet is set in graphene sensor.
Further, it is arranged on the upper wall of the separating liquid pipeline at the third giant magnetoresistance chip module rear to electrode,
Graphene sensor is set on the lower wall for being equipped with the separating liquid pipeline opposite to electrode middle position.
Further, it is arranged on the separating liquid pipeline between second circulation motor and third giant magnetoresistance chip module
Detection control solenoid valve.
Further, the end of the separating liquid pipeline is connect with waste collecting device.
Further, further include controller and electrochemical workstation, controller include MCU unit, energy supply control module and
Signal processing module, detection control solenoid valve, the 5th electromagnet pass through energy supply control module with second circulation motor respectively and connect
To MCU unit, electrochemical workstation and third giant magnetoresistance chip module pass through signal processing module respectively and are connected to MCU unit,
MCU unit is connect with power module.
A kind of electrochemical immunoanalytical instrument, including above-mentioned online electrochemical detection device.
A kind of detection method of online electrochemical detection device mainly includes following steps：
S1, the sterile water containing immunomagnetic beads enter separating liquid pipeline, open detection control solenoid valve, open second circulation horse
It reaches, third giant magnetoresistance chip module detects the measurement of magnetic bead quantity, is transferred in MCU unit the meter that adds up through signal processing module
It calculates；
S2, the 5th electromagnet is opened, the immunomagnetic beads for entering pipeline is adsorbed in graphene sensor, when the huge magnetic of third
When resistance chip module detects that the average value of immunomagnetic beads concentration is less than preset threshold d, show that all magnetic beads have all entered detection
Device closes detection control solenoid valve and second circulation motor, saves the accumulated value d at current time；
S3, starting electrochemical workstation, electric signal are real-time by the amplification of signal processing module, filtering feeding MCU unit
Display, monitoring；
S4, it completes after detecting, open detection control solenoid valve and second circulation motor are passed through cleaning solution to pipeline cleaning.
Compared with the existing technology, a kind of online electrochemical detection device, method described in the invention and electrochemistry are exempted from
Epidemic disease analyzer has the advantage that：
(1) analyzer described in the invention carries out precise volume to immunomagnetic beads by using giant magnetoresistance chip module
Change, reduces test error, remain effective test sample to the greatest extent, improve the sensitivity of instrument, improve inspection
Efficiency is surveyed, and multiple giant magnetoresistance chip modules are arranged in equipment from front to back, by the detection to different point immunomagnetic beads, to anti-
It answers, the test error that may cause of magnetic bead is lost in separation process compensated, keep test data more accurate, improve equipment
Precision, reduce testing cost, the device structure is compact, is conducive to a wide range of promote.
(2) detection device described in the invention is detected using graphene sensor, improves the sensitive of detection device
It spends, solution constant flow in detection process, real-time is good, realizes on-line checking.
Detailed description of the invention
The attached drawing for constituting a part of the invention is used to provide to further understand the invention, present invention wound
The illustrative embodiments and their description made are used to explain the present invention creation, do not constitute the improper restriction to the invention.?
In attached drawing：
Fig. 1 is the structural schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of reaction unit part；
Fig. 3 is the structural block diagram for controlling reaction unit；
Fig. 4 is the structural schematic diagram of separator part；
Fig. 5 is the structural block diagram for controlling separator；
Fig. 6 is the structural schematic diagram of detection device part.
Description of symbols：
1, input duct；11, sample pipe；111, sample pipe solenoid valve；12, immunomagnetic beads pipeline；121, magnetic is immunized
Pearl pipe electromagnetic valve；13, cleaning fluid pipe；131, cleaning fluid pipe solenoid valve；14, the total solenoid valve of input duct；
2, reaction unit；21, reacting pipe；22, first circulation motor；23, the first giant magnetoresistance chip module；24, first
Electromagnet；25, the second electromagnet；
3, reaction solution pipeline；31, the first waste solution channel；311, waste solution channel solenoid valve；32, the second waste solution channel；321,
Second waste solution channel solenoid valve；33, the total solenoid valve of reaction solution pipeline；34, protruding portion；
4, separator；41, third electromagnet；42, the total solenoid valve of separate pipeline；43, sterile waterpipe；431, sterile water
Pipe electromagnetic valve；44, the 4th electromagnet；45, the second giant magnetoresistance chip module；
5, separating liquid pipeline；51, separating liquid pipe electromagnetic valve；
6, detection device；61, second circulation motor；62, third giant magnetoresistance chip module；63, to electrode；64, graphene
Sensor；65, the 5th electromagnet；66, detection control solenoid valve.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the invention can
To be combined with each other.
In the description of the invention, it is to be understood that term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description the invention and simplifies description, rather than indicate
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For the limitation to the invention.In addition, term " first ", " second " etc. are used for description purposes only, and should not be understood as indicating
Or it implies relative importance or implicitly indicates the quantity of indicated technical characteristic." first ", " second " etc. are defined as a result,
Feature can explicitly or implicitly include one or more of the features.In the description of the invention, unless separately
It is described, the meaning of " plurality " is two or more.
In the description of the invention, it should be noted that unless otherwise clearly defined and limited, term " peace
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the invention.
The present invention will be described in detail below with reference to the accompanying drawings and embodiments creates.
As shown in Figure 1, a kind of electrochemical immunoanalytical instrument, including pass through the sequentially connected reaction unit 2 of pipeline, separation dress
Set 4 and detection device 6, as shown in Fig. 2, reaction unit 2 include annular reacting pipe 21, one end of reacting pipe 21 be arranged into
Liquid mouth, inlet are connected to input duct 1,1 other end of input duct respectively with sample pipe 11, immunomagnetic beads pipeline 12 and clear
Washing lotion pipeline 13 is connected to, and the solenoid valve of control pipeline opening and closing is respectively set on each pipeline, have respectively sample pipe solenoid valve 111,
Immunomagnetic beads pipe electromagnetic valve 121, cleaning fluid pipe solenoid valve 131 and the total solenoid valve 14 of input duct, reacting pipe 21 are another
End setting liquid outlet, liquid outlet are connected to reaction solution pipeline 3, the series connection access first circulation motor on the reacting pipe 21 of side
22, the first giant magnetoresistance chip module 23 is arranged on the reacting pipe 21 ipsilateral with first circulation motor 22, in the anti-of the other side
It answers and the first electromagnet 24 is set above pipeline 21, the second electromagnet 25 is set on the reaction solution pipeline 3 close to liquid outlet direction.
The first waste solution channel 31 for cleaning solution waste liquid to be discharged is set on reaction solution pipeline 3, on the first waste solution channel 31
First waste solution channel solenoid valve 311 is set, the total solenoid valve 33 of reaction solution pipeline is set on reaction solution pipeline 3, reaction solution pipeline 3
The other end connects separator 4.
As shown in figure 4, separator 4 includes the sterile waterpipe 43 being connected to 3 other end of reaction solution pipeline and separating liquid
Pipeline 5, sterile waterpipe 43 and 5 coaxial inner conductor of separating liquid pipeline and vertical with the axial line of reaction solution pipeline 3, in sterile water pipe
Second waste solution channel 32, sterile waterpipe 43, separating liquid are set on the reaction solution pipeline 3 between road 43 and the first waste solution channel 31
Sterile water pipe electromagnetic valve 431, the separating liquid pipeline for controlling each pipeline on-off are respectively set on pipeline 5 and the second waste solution channel 32
Solenoid valve 51 and the second waste solution channel solenoid valve 321, the reaction liquid pipe between the second waste solution channel 32 and sterile waterpipe 43
Several third electromagnet 41 are set on road 3, and third electromagnet 41 is connected to controller, controller energy by energy supply control module
It is enough so that immunomagnetic beads is from left to right gradually moved to reaction solution pipeline 3 according to the on-off of certain timing control third electromagnet 41
The total solenoid valve 42 of separate pipeline is arranged on the separating liquid pipeline 5 on the right side of right end third electromagnet 41 in end.
The prominent sterile waterpipe 43 in 3 end of reaction solution pipeline forms protruding portion 34, and the 4th electromagnet is arranged on protruding portion 34
44, immunomagnetic beads can be adsorbed on the side wall of the sterile waterpipe 43 joined with reaction solution pipeline 3 by the 4th electromagnet 44, nothing
Sterile water is passed through in bacterium waterpipe 43 to clean immunomagnetic beads, and the second giant magnetoresistance chip dies are arranged on separating liquid pipeline 5
Group 45, the second giant magnetoresistance chip module 45 is connected to controller.
Third electromagnet 41 is from left to right uniform to be arranged on reaction solution pipeline 3, third electromagnet 41 be electromagnetism ring or
Person's bare electromagnet or gradient electromagnetic field mould group.
4th electromagnet 44 is electromagnetism ring, and protruding portion 34 is blind pipe, and electromagnetism ring is set on blind pipe or the 4th electromagnet
44 be bare electromagnet, and protruding portion 34 is flat tube, and bare electromagnet is fixed in flat tube.
Detection device 6 is arranged in the other end of separating liquid pipeline 5, as shown in fig. 6, detection device 6 includes being connected on separating liquid
Second circulation motor 61 in pipeline 5 sets gradually detection control electricity on the separating liquid pipeline 5 at 61 rear of second circulation motor
Magnet valve 66 and third giant magnetoresistance chip module 62, on the upper wall of the separating liquid pipeline 5 at 62 rear of third giant magnetoresistance chip module
And graphene sensor is arranged on the lower wall for being equipped with the separating liquid pipeline 5 opposite to 63 middle position of electrode to electrode 63 in setting
64, the 5th electromagnet 65 is set below graphene sensor 64, the setting opposite to electrode 63 and graphene sensor 64 point
On the tube wall of chaotropic pipeline 5, the end of separating liquid pipeline 5 is connect with waste collecting device, and detection device 6 further includes electrification
Work station is learned, electrochemical workstation is connected to controller.
The quantity of third electromagnet 41 can depending on the specification of pipeline, separator 4 can series multistage use to increase
Add the wash number to immunomagnetic beads.
As shown in Figure 3 and Figure 5, which further includes controller, and controller includes MCU unit, energy supply control module and letter
Number processing module, solenoid valve, electromagnet and circulation motor in all pipelines pass through energy supply control module to be respectively connected to MCU mono-
Member, all giant magnetoresistance chip modules, electrochemical workstation pass through signal processing module and are connected to MCU unit, MCU unit with
Power module connection, power module provide electric energy for MCU unit.
The analysis method of electrochemical immunoanalytical instrument mainly includes following steps：
Reaction process：Drive immunomagnetic beads in reacting pipe 21 using first circulation motor 22, electromagnet, each solenoid valve
It mixes, reaction, using the on-off enrichment and separation magnetic bead of electromagnet and solenoid valve, is detected using the first giant magnetoresistance chip module 23
The quantity of immunomagnetic beads cleans pipeline using cleaning solution, collects immunomagnetic beads.
MCU unit opens sample pipe solenoid valve 111, immunomagnetic beads pipe electromagnetic valve 121 and the total solenoid valve of input duct
14, first circulation motor 22 is opened, sample solution and immunomagnetic beads solution are mixed into input duct 1, by T1 time, sample-adding
It finishes, closes sample pipe solenoid valve 111, at the same time, signal processing module acquires the detection of the first giant magnetoresistance chip module 23
The signal of immunomagnetic beads quantity, signal processing module will acquire signal and be input to MCU unit progress accumulation process, when accumulated value reaches
When to given threshold a, immunomagnetic beads solution is enough, closes immunomagnetic beads pipe electromagnetic valve 121 and the total solenoid valve of input duct
14。
Using T2 as the on-off of the first electromagnet of time interval controls 24, first circulation motor 22 quickly drives mixed MCU unit
It closes solution to circulate in reacting pipe 21, the interval on-off of the first electromagnet 24 can change immunomagnetic beads in the solution
Motion profile forms turbulent flow, so that immunomagnetic beads and sample solution is reacted more abundant, after the T3 time, end of reaction is closed
Close the first electromagnet 24.
MCU unit opens the second electromagnet 25, and immunomagnetic beads continue under the slow drive of first circulation motor 22 anti-
It answers in pipeline and circulates, by being attracted when the second 25 position of electromagnet, be gathered in 21 exit of reacting pipe, meanwhile,
One giant magnetoresistance chip module 23 measures the concentration of immunomagnetic beads in reacting pipe 21, when mean intensity value is lower than given threshold b,
It is believed that magnetic bead has been completely segregated, cleaning fluid pipe solenoid valve 131 is opened, the first waste solution channel solenoid valve 311 is opened, clear
Washing lotion pipeline 13 is passed through cleaning solution, and cleaning input duct 1, reacting pipe 21 and immunomagnetic beads, waste liquid is via the first waste solution channel
31 discharges.
The T4 time is waited, after the completion of cleaning, the second electromagnet 25 is closed, closes the first waste solution channel solenoid valve 311, open
The total solenoid valve 33 of reaction solution pipeline, sterile water enter input duct 1 via cleaning fluid pipe 13, and immunomagnetic beads is driven to flow to point
From device 4.
Separation process：Controller controls several third electromagnet 41 according to certain timing on-off, passes through the rule of generation
It restrains electromagnetic field and drives mobile separation of the immunomagnetic beads in mixed solution, the second giant magnetoresistance chip module 45 detects immunomagnetic beads
Quantity.
For reaction solution in reaction solution pipeline 3, MCU unit controls each third electromagnet 41 according to certain timing individually or simultaneously
Magnetic field is generated, from left to right successively absorption immunomagnetic beads keep it mobile to 3 right end of reaction solution pipeline.
The first electromagnet 41 of left end first is powered, and the immunomagnetic beads in reaction solution pipeline 3 are adsorbed onto the first electromagnet
At pipeline among 24；It keeps first, left side third electromagnet 41 to be powered, opens the third electromagnet 41 of left end second, will exempt from
Epidemic disease magnetic bead is adsorbed onto the position among two magnet rings；The third electromagnet 41 of left end first is disconnected, second third electromagnet is kept
41 are powered, and immunomagnetic beads are adsorbed onto the position among second third electromagnet 41；Successively on-off residue electromagnet, until exempting from
Epidemic disease magnetic bead is moved to the position among the third electromagnet 41 of right end, opens the total solenoid valve 42 of separate pipeline, opens the 4th electricity
Magnet 44 opens the second waste solution channel solenoid valve 321, opens sterile water pipe electromagnetic valve 431 and injects sterile water, in the 4th electromagnetism
Under the collective effect of the vertical direction impact force of the horizontal direction attraction and sterile water of iron 44, immunomagnetic beads enter separation liquid pipe
Road 5, part sterile water will be redirected back into reaction solution pipeline 5 and be discharged from the second waste solution channel 32, complete the cleaning of immunomagnetic beads
And separation.
Second giant magnetoresistance chip module 45 acquires the signal of immunomagnetic beads quantity, is transmitted to MCU by signal processing module
Unit shows that most of magnetic bead has been collected when magnetic bead mean concentration is less than given threshold c in solution, opens separating liquid
Immunomagnetic beads are drained into detection device 6, close separating liquid pipe electromagnetic valve 51, continue to be passed through sterile water, beat by pipe electromagnetic valve 51
The second waste solution channel solenoid valve 321 is opened, the cleaning to each pipeline in separator 4 is completed.
Detection process：It drives immunomagnetic beads solution by separating liquid pipeline 5 using second circulation motor 61, uses the 5th electricity
Immunomagnetic beads are adsorbed on 64 surface of graphene sensor by magnet 65, detection are realized in electrochemical workstation, and will test letter
Number being transmitted to MCU unit by signal processing module is shown, participates in detection by 62 pairs of third giant magnetoresistance chip module
The quantity of immunomagnetic beads measures, thus the detection caused by the immunomagnetic beads being lost in pretreatment process in concentration calculation
Error compensates, and improves detection accuracy.
Detection control solenoid valve 66 is opened, second circulation motor 61 is opened and immunomagnetic beads solution is driven to lead in detection device 6,
Immunomagnetic beads are by the magnet magnetized rear generation electromagnetic field in third giant magnetoresistance chip module 62, so as to realize to magnetic bead quantity
Measurement, be transferred to accumulation calculating in MCU unit through signal processing module, open third electromagnet 41, exempting from for pipeline will be entered
Epidemic disease magnetic bead is adsorbed in graphene sensor 64.
When third giant magnetoresistance chip module 62 detects the average value of immunomagnetic beads concentration less than preset threshold d, show
All magnetic beads have all entered detection device 6, close detection control solenoid valve 66 and second circulation motor 61, save current time
Accumulated value d.
Start electrochemical workstation, electric signal is sent into MCU unit by amplification, the filtering of signal processing module.
After completing detection, open detection control solenoid valve 66 and second circulation motor 61 and to be passed through cleaning solution clear to pipeline
It washes.
The analyzer carries out precise quantification to immunomagnetic beads by using giant magnetoresistance chip module, reduces test error,
Effective test sample is remained to the greatest extent, improves the sensitivity of instrument, improves detection efficiency, and equipment arrives in the past
After multiple giant magnetoresistance chip modules are set, by the detection to different point immunomagnetic beads, to losing magnetic in reaction, separation process
The test error that pearl may cause is compensated, and keeps test data more accurate, improves the precision of equipment, reduce detection at
This, the device structure is compact, is conducive to a wide range of promote.
Reaction unit 2 by control of the MCU controller to each solenoid valve and each electromagnet, realize automation reaction and
Lock out operation, controller control each electromagnet timing on-off and cooperate first circulation motor 22, make immunomagnetic beads and sample solution
It comes into full contact with, reacts more thorough, by the way that electromagnet is arranged in 21 exit of annular reaction pipeline, while solution circulation flow is dynamic,
Keep the separation of immunomagnetic beads more thorough.
Separator 4 drives immunomagnetic beads to 3 end of reaction solution pipeline by carrying out the control of timing on-off to multiple electromagnet
End motion, compared with common liquid drives, separation is more thorough, after separating magnetic bead, carries out back flush to separate lines,
Pipeline-cleaning is more thorough, with it is traditional take liquid needle to design compared with, realize online separation, separating rate faster, improves point
From efficiency.
Detection device 6 is detected using graphene sensor 64, improves the sensitivity of detection device 6, molten in detection process
Liquid constant flow, real-time is good, realizes on-line checking.
The foregoing is merely the preferred embodiments of the invention, are not intended to limit the invention creation, all at this
Within the spirit and principle of innovation and creation, any modification, equivalent replacement, improvement and so on should be included in the invention
Protection scope within.
Claims (7)
1. a kind of online electrochemical detection device, it is characterised in that：Including separating liquid pipeline (5), on separating liquid pipeline (5) in the past
Second circulation motor (61), third giant magnetoresistance chip module (62) are set gradually after and to electrode (63), and be equipped with to electrode
(63) graphene sensor (64) are set on the opposite tube wall of separating liquid pipeline (5), the is arranged in graphene sensor (64)
Five electromagnet (65).
2. online electrochemical detection device according to claim 1, it is characterised in that：In the third giant magnetoresistance chip dies
Setting is opposite to electrode (63) middle position with being equipped with to electrode (63) on the upper wall of the separating liquid pipeline (5) at group (62) rear
Separating liquid pipeline (5) lower wall on be arranged graphene sensor (64).
3. online electrochemical detection device according to claim 1, it is characterised in that：It is described in second circulation motor (61)
Setting detection control solenoid valve (66) on separating liquid pipeline (5) between third giant magnetoresistance chip module (62).
4. online electrochemical detection device according to claim 1, it is characterised in that：The end of the separating liquid pipeline (5)
End is connect with waste collecting device.
5. online electrochemical detection device according to claim 1, it is characterised in that：It further include controller and electrochemistry work
It stands, controller includes MCU unit, energy supply control module and signal processing module, detection control solenoid valve (66), the 5th electromagnetism
Iron (65) and second circulation motor (61) are connected to MCU unit, electrochemical workstation and third by energy supply control module respectively
Giant magnetoresistance chip module (62) is connected to MCU unit by signal processing module respectively, and MCU unit is connect with power module.
6. a kind of electrochemical immunoanalytical instrument, it is characterised in that：Including the described in any item online electrochemistry inspections of claim 1-5
Survey device.
7. a kind of detection method of online electrochemical detection device, it is characterised in that：It mainly include following steps：
S1, the sterile water containing immunomagnetic beads enter separating liquid pipeline (5), open detection control solenoid valve (66), open second and follow
Ring motor (61), third giant magnetoresistance chip module (62) detect the measurement of magnetic bead quantity, it is mono- to be transferred to MCU through signal processing module
Accumulation calculating in member；
S2, the 5th electromagnet (65) is opened, the immunomagnetic beads for entering pipeline is adsorbed on graphene sensor (64), third is worked as
When giant magnetoresistance chip module (62) detects that the average value of immunomagnetic beads concentration is less than preset threshold d, show all magnetic beads all
Into detection device (6), detection control solenoid valve (66) and second circulation motor (61) are closed, the accumulated value at current time is saved
d；
S3, starting electrochemical workstation, electric signal passes through the amplification of signal processing module, MCU unit real-time display is sent into filtering,
Monitoring；
S4, after completing detection, to be passed through cleaning solution clear to pipeline for open detection control solenoid valve (66) and second circulation motor (61)
It washes.
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