CN109077957A - It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution and its preparation and application - Google Patents
It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution and its preparation and application Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention disclose it is a kind of have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution and its preparation and application, belong to cosmetic field.The Chinese medicine composition fermentation liquid used for cosmetic is by including that the traditional Chinese medicinal components of following parts by weight are made: 10~40 parts of the sophora bud, 5~30 parts of ginkgo leaf, 5~30 parts of wild chrysanthemum, 5~30 parts of the achene of Siberian cocklebur, 5~30 parts of Radix Glycyrrhizae.The Chinese medicine composition fermentation liquid used for cosmetic has the characteristics that natural safety.The present invention is that guidance carries out prescription with Chinese medicine monarch theory, is acted synergistically by plurality of Chinese, obtains resistant to pollution purpose in terms of the comprehensive effect such as clearing heat and detoxicating, removing free radical, antiallergic, uvioresistant, antibacterial, promotion skin healing.By scientific composition, Chinese medicine composition fermentation liquid of the invention is applied to manufactured cosmetics in cosmetics, has effects that injury of the good anti-air pollution to skin.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition fermentation liquids, specifically a kind of to have effects that the used for cosmetic of antipollution
Chinese medicine composition fermentation liquid and its preparation and application, belong to cosmetic field.
Background technique
In recent years, skin sufferer number sharply increases, and traces it to its cause very much, Air Contamination is therein important one
Ring, air pollution are easy to cause following skin problem: first is that leading to skin allergy: the floating material allergy of a large amount of disperses in air,
Cause skin pore blocking and allergic symptom, make one to feel itch, plays erythema wheal, or even triggering nettle rash and eczema.Second is that
Cause the aging of skin: pollutants in air, such as haze that skin can be made to generate the extremely unstable free radical of excess, these are certainly
By base by the DNA of damage cell membrane and human body own health, thus accelerate organism aging process, the aging including skin, such as skin
Obscure, pigmentation, skin are easy relaxation, microgroove or wrinkle occur, have dry skin, even result in skin canceration.Whether three lead
Cause skin to be easy infection: haze causes the particle in air excessive, and degree of light transmission can reduce, and makes the ultraviolet light for reaching Near Ground
It is weakened.Various viruses in near-earth air are swum in this way, and bacterium and fungi become to be not easy to be killed by ultraviolet light.For constitution
It is poor, there is a skin allergy, for the people of skin injury, this quasi-microorganism reaches skin with the dust particles of air, and adheres to
In skin surface, the infection of skin, such as epifolliculitis, sweat stain are easily caused, herpe simplex etc. has the people of fash that can aggravate.
So to resist injury of the outside contamination to skin, should from toxin expelling, antiallergic, remove free radical, isolation ultraviolet,
Antibacterial and cleaning etc. is set about.
Summary of the invention
In order to overcome the disadvantages and deficiencies of the prior art, in conjunction with Traditional Chinese medical theory basis, primary and foremost purpose of the invention exists
A kind of has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution in providing.The Chinese medicine composition fermentation liquid used for cosmetic
Have the characteristics that natural safe.
Another object of the present invention is to provide a kind of above-mentioned Chinese medicine composition used for cosmetic hairs with antipollution
The preparation method of zymotic fluid.
Has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution a further object of the present invention is to provide a kind of
Application.
The purpose of the invention is achieved by the following technical solution:
It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by including in following parts by weight
Medicine component is made: 10~40 parts of the sophora bud, 5~30 parts of ginkgo leaf, and 5~30 parts of wild chrysanthemum, 5~30 parts of the achene of Siberian cocklebur, Radix Glycyrrhizae 5~30
Part.
Preferably, described to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by including following weight
The traditional Chinese medicinal components of number are made: 25~30 parts of the sophora bud, 15~20 parts of ginkgo leaf, and 17~20 parts of wild chrysanthemum, 18~20 parts of the achene of Siberian cocklebur,
10~20 parts of Radix Glycyrrhizae.
Preferably, described to have effects that the Chinese medicine composition fermentation liquid (I) used for cosmetic of antipollution, by including following heavy
The traditional Chinese medicinal components of amount number are made: 28 parts of the sophora bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum, 18 parts of the achene of Siberian cocklebur, 17 parts of Radix Glycyrrhizae.
Preferably, described to have effects that the Chinese medicine composition fermentation liquid (II) used for cosmetic of antipollution, by including following
The traditional Chinese medicinal components of parts by weight are made: 30 parts of the sophora bud, 20 parts of ginkgo leaf, 20 parts of wild chrysanthemum, 20 parts of the achene of Siberian cocklebur, 10 parts of Radix Glycyrrhizae.
Preferably, described to have effects that the Chinese medicine composition fermentation liquid (III) used for cosmetic of antipollution, by including following
The traditional Chinese medicinal components of parts by weight are made: 25 parts of the sophora bud, 15 parts of ginkgo leaf, 20 parts of wild chrysanthemum, 20 parts of the achene of Siberian cocklebur, 20 parts of Radix Glycyrrhizae.
Preferably, compound bacteria used in the fermentation is lactobacillus plantarum and S. cervisiae；
Preferably, lactobacillus plantarum and S. cervisiae be in proportion (1~5) in the compound bacteria: 1 composition, compound bacteria
Liquid concentration is 106~108cfu/mL。
The second object of the present invention, which is to provide, above-mentioned has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution
Preparation method.
To reach this purpose of the invention, present invention employs fermentation extraction methods, i.e., Chinese medicine composition ferment
After processing, Chinese medicine composition fermentation liquid is obtained using filter, sterilizing and centrifugal separation.Specifically comprise the following steps:
1. compound bacteria is added in the form of bacterium solution, the composite bacteria liquid be lactobacillus plantarum and S. cervisiae in proportion
(1~5): 1 composition, composite bacteria liquid concentration are 106~108cfu/mL；
2. stock: by the removal of impurities of each Chinese medicine material, cleaning, drying, being crushed to 80~200 mesh, mix, obtain Chinese traditional medicine composition
Object；Compound concentration is 106~108The composite bacteria liquid of cfu/mL, pH value are adjusted to 6.5~7.5；
3. fermentation: according to the ratio mixing composite bacteria liquid, Chinese medicine composition and water, in 28~42 DEG C, 50~300r/min item
It ferments 36~48 hours in part, obtains fermentation liquid；
4. purification: by gained filtering fermentation liquor, filtrate makes in 105~120 DEG C of at a temperature of 20~35min of high pressure sterilization
Strain inactivation；Ferment filtrate after sterilizing is centrifuged 20 under conditions of 3000~8000r/min, centrifugation radius are 5~20cm
~35min abandons precipitating, collects supernatant, is concentrated with film, concentrate macroporous resin adsorption, then with volume fraction for 10~
95% ethyl alcohol repeatedly elutes, and collects eluent, and film is concentrated into the concentrate with Chinese medicine composition identical weight, add with it is dense
The butanediol of contracting liquid identical weight dissolves, and filtering as has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution.
Preferably, step 1. described in composite bacteria liquid be that 3:1 is formed in proportion for lactobacillus plantarum and S. cervisiae；
Preferably, step 3. in, ferment 36~48 hours in 28~35 DEG C, 100r/min condition, obtain fermentation liquid；
The amount ratio of the composite bacteria liquid, Chinese medicine composition and water is 20mL~60mL:20g~60g:200~600g.
Preferably, the amount ratio of the composite bacteria liquid, Chinese medicine composition and water is 25mL~40mL:30g~45g:300
~400g.
Preferably, the amount ratio of the Chinese medicine composition fermentation liquid (I), composite bacteria liquid, Chinese medicine composition and water is 30mL:
40g:300g.
Preferably, the Chinese medicine composition fermentation liquid (II), the amount ratio of composite bacteria liquid, Chinese medicine composition and water are
40mL:45g:400g.
Preferably, the Chinese medicine composition fermentation liquid (III), the amount ratio of composite bacteria liquid, Chinese medicine composition and water are
25mL:30g:300g.
Preferably, step 4. described in centrifugation radius be 8~12cm.
Preferably, step 4. in, filtrate inactivates strain in 110~120 DEG C of at a temperature of high pressure sterilization 30min；It will go out
Ferment filtrate after bacterium is centrifuged 30min under conditions of 6000~8000r/min, centrifugation radius are 9cm, abandons and precipitates, in collection
Clear liquid.
The third object of the present invention, which is to provide, has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution is being made up
Application in product.
To reach this purpose of the invention, present invention employs following technical solutions: described to have effects that antipollution
Chinese medicine composition fermentation liquid used for cosmetic is preparing the application in cosmetics, has effects that antipollution containing above-mentioned in cosmetics
Chinese medicine composition fermentation liquid used for cosmetic, additive amount are 0.1~15% based on mass fraction.
The present invention also provides a kind of cosmetics, which, which contains, above-mentioned has effects that the Chinese medicine group used for cosmetic of antipollution
Close object fermentation liquid.
Preferably, which, which contains, above-mentioned has effects that the makeup of antipollution based on mass fraction for 0.1~15%
Product Chinese medicine composition fermentation liquid.
The cosmetics are as follows: aqua, type of latex type, cream kind, gel class, powder class, powder compacting class, facial mask, spray-like,
Essence class washes shield class.
Specifically, the present invention provides a kind of antipollution facial mask, the component including following mass fraction meter:
| Component | Mass fraction/% |
| Chinese medicine composition fermentation liquid of the invention | 5 |
| Glycerol | 6.00 |
| 1,3- propylene glycol | 4.00 |
| Sodium Hyaluronate | 0.10 |
| Xanthan gum | 0.10 |
| Hydroxyethyl cellulose | 0.10 |
| Dipotassium glycyrrhizinate | 0.50 |
| Aloe extract | 1.00 |
| 1,2- hexylene glycol | 0.80 |
| Parahydroxyacet-ophenone | 0.50 |
| Deionized water | Surplus |
Specifically, the present invention provides a kind of antipollution face cream, the component including following mass fraction meter:
| Ingredient | Mass fraction (%) |
| Arachidic alcohol glucoside | 1.5 |
| PEG-100 stearate | 1.2 |
| Stearine | 1 |
| Cetearyl glucoside | 0.8 |
| Shea butter | 0.5 |
| White pond flower seed oil | 3 |
| Parleam | 3 |
| Cyclomethicone | 4 |
| Dimethyl silicone polymer | 2 |
| Methyl hydroxybenzoate | 0.2 |
| Nipasol | 0.08 |
| EDTA‐2Na | 0.02 |
| Glycerol | 5 |
| 1,3 butylene glycol | 3 |
| Sodium Hyaluronate | 0.05 |
| Carbomer (CA) | 0.2 |
| Phenoxyethanol | 0.3 |
| Aminomethyl propanol | 0.12 |
| Essence | 0.1 |
| Chinese medicine composition fermentation liquid of the invention | 5 |
| Deionized water | Surplus |
Mechanism of the invention is:
Traditional Chinese medicine beautifying theory thinks, good skin not only needs external modification, it is often more important that skin is from inside to outside
Health.Although the reason of forming skin problem be it is various, strong skin itself be to a certain extent can resist it is outer
Boundary's stimulation, so, Chinese medicine antipollution is the process of a comprehensively regulating, is to be resisted by enhancing the immunity of skin to reach
The ability of outside contamination.
The present invention is that guidance carries out prescription with Chinese medicine monarch theory, and using the sophora bud as monarch drug in a prescription, the sophora bud is to toxin expelling and acceleration
Metabolism has special effect, can accelerate skin metabolism toxin expelling, and has extraordinary toxin-expelling and face nourishing, free radical resisting, resists
Ultraviolet light and repair tissue regeneration effect；Ginkgo leaf and wild chrysanthemum are ministerial drug jointly, and ginkgo leaf has very strong removing free
Base, anti-aging, strengthen immunity and good antiallergic effect, wild chrysanthemum have broad-spectrum antibacterial action, have to Propiobacterium etc.
Very strong inhibiting effect；The achene of Siberian cocklebur is adjutant, has very strong relieving exterior syndrome and dispelling cold, expelling wind and eliminating dampness, itching-relieving efficacies；Radix Glycyrrhizae is to make
Medicine has clearing heat and detoxicating, anti-inflammatory, free radical resisting and other effects, has the function of coordinating the drug actions of a prescription.The above all medicines share, this strong solid member,
Conditioning is nourished, using strong skin as antipollution sheet；To clean toxin expelling, anti-inflammatory sterilization, antiallergic of releiving is the mark of antipollution.This hair
Bright Chinese medicine composition fermentation liquid has extraordinary clearing heat and detoxicating, removing free radical, antiallergic, uvioresistant, antibacterial, promotion skin
The comprehensive effect such as skin healing, makes an addition in cosmetics, anti-air pollution obvious effect.
Can change material composition molecular structure especially with fermentation extraction method, promote raw material effect, reduce toxicity and
Irritation, the advantages such as optimization color, taste, is a kind of comparatively ideal traditional Chinese medicine extraction new process, is conducive to promote and apply.
The present invention has the following advantages and effects with respect to the prior art:
The present invention is that guidance carries out prescription with Chinese medicine monarch theory, is acted synergistically by plurality of Chinese, from heat-clearing solution
Poison obtains resistant to pollution purpose in terms of removing the comprehensive effect such as free radical, antiallergic, uvioresistant, antibacterial, promotion skin healing.
By scientific composition, Chinese medicine composition fermentation liquid of the invention is applied to manufactured cosmetics in cosmetics, is had good
The injury effect of anti-air pollution to skin.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
The test method of specific experiment condition is not specified in the following example, usually according to conventional laboratory conditions or according to system
Make experiment condition proposed by factory.
Lactobacillus plantarum used in embodiment (Lactobacillus plantarum), adds biology purchased from Changzhou beneficial bacteria
Science and Technology Ltd., model NJ2205；
S. cervisiae used in embodiment (Saccharomyces cerevisiae) is purchased from the triumphant microorganism of Guangdong ring
Science and Technology Ltd., model ATCC9763；
Propionibacterium acnes used in embodiment (Propionibacterium acnes) are purchased from the triumphant micro- life of Guangdong ring
Object Science and Technology Ltd., model GIM1.162.
Embodiment 1 is a kind of to have effects that the Chinese medicine composition fermentation liquid (I) used for cosmetic of antipollution and preparation method
It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by the Chinese medicine including following parts by weight
Material component is made: 28 parts of the sophora bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum, 18 parts of the achene of Siberian cocklebur, 17 parts of Radix Glycyrrhizae.
The preparation method is as follows:
1. compound bacteria is added in the form of bacterium solution, the composite bacteria liquid be lactobacillus plantarum and S. cervisiae in proportion
3:1 composition, composite bacteria liquid concentration are 106~108cfu/mL。
2. stock: Chinese medicine total weight is 100g, by the removal of impurities of each Chinese medicine material, cleaning, drying, is crushed to 100 mesh, mixes,
Obtain Chinese medicine composition；Compound concentration is 106~108The composite bacteria liquid of cfu/mL, pH value are adjusted to 6.5~7.5.
3. fermentation: according to the ratio mixing composite bacteria liquid, Chinese medicine composition and water, sent out in 32 DEG C, the shaking table of 100r/min
Ferment 40 hours, obtain fermentation liquid.
4. purification: by gained filtering fermentation liquor, filtrate inactivates strain in 110 DEG C of at a temperature of high pressure sterilization 30min.
Ferment filtrate after sterilizing is centrifuged 30min under conditions of 6000r/min, centrifugation radius are 9cm, precipitating is abandoned, collects supernatant
Liquid is concentrated with film, and concentrate is with macroreticular resin (AB-8 macroporous absorbent resin, be purchased from Tianjin pula moral Biotechnology Co., Ltd)
Absorption, then repeatedly eluted with the ethyl alcohol that volume fraction is 10~95%, eluent is collected, film is concentrated into identical as Chinese medicine composition
The concentrate of weight is added and is dissolved with the butanediol of concentrate identical weight, Chinese medicine composition fermentation liquid as of the invention
(Ⅰ)。
Wherein, the amount ratio of composite bacteria liquid, Chinese medicine composition and water is 30mL:40g:300g.
Embodiment 2 is a kind of to have effects that the Chinese medicine composition fermentation liquid (II) used for cosmetic of antipollution and preparation method
It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by the Chinese medicine including following parts by weight
Material component is made: 30 parts of the sophora bud, 20 parts of ginkgo leaf, 20 parts of wild chrysanthemum, 20 parts of the achene of Siberian cocklebur, 10 parts of Radix Glycyrrhizae.
The preparation method is as follows:
1. compound bacteria is added in the form of bacterium solution, the composite bacteria liquid be lactobacillus plantarum and S. cervisiae in proportion
3:1 composition, composite bacteria liquid concentration are 106~108cfu/mL。
2. stock: Chinese medicine total weight is 100g, and each Chinese medicine material removal of impurities, cleaning, drying are crushed to 80 mesh, mix, obtain
Chinese medicine composition；Compound concentration is 106~108The composite bacteria liquid of cfu/mL, pH value are adjusted to 6.5~7.5.
3. fermentation: according to the ratio mixing composite bacteria liquid, Chinese medicine composition and water, sent out in 35 DEG C, the shaking table of 100r/min
Ferment 48 hours, obtain fermentation liquid.
4. purification: by gained filtering fermentation liquor, filtrate inactivates strain in 110 DEG C of at a temperature of high pressure sterilization 30min.
Ferment filtrate after sterilizing is centrifuged 30min under conditions of 6000r/min, centrifugation radius are 9cm, precipitating is abandoned, collects supernatant
Liquid is concentrated with film, concentrate macroporous resin adsorption, then is repeatedly eluted with the ethyl alcohol that volume fraction is 10~95%, and collection is washed
De- liquid, film are concentrated into the concentrate with Chinese medicine composition identical weight, add and dissolve with the butanediol of concentrate identical weight,
Filtering, Chinese medicine composition fermentation liquid (II) as of the invention.
Wherein, the amount ratio of composite bacteria liquid, Chinese medicine composition and water is 40mL:45g:400g
Embodiment 3 has effects that the Chinese medicine composition fermentation liquid (III) used for cosmetic of antipollution and preparation method
It is a kind of to have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by the Chinese medicine including following parts by weight
Material component is made: 25 parts of the sophora bud, 15 parts of ginkgo leaf, 20 parts of wild chrysanthemum, 20 parts of the achene of Siberian cocklebur, 20 parts of Radix Glycyrrhizae.
The preparation method is as follows:
1. compound bacteria is added in the form of bacterium solution, the composite bacteria liquid be lactobacillus plantarum and S. cervisiae in proportion
3:1 composition, composite bacteria liquid concentration are 106~108cfu/mL；
2. stock: Chinese medicine total weight is 100g, by the removal of impurities of each Chinese medicine material, cleaning, drying, is crushed to 200 mesh, mixes,
Obtain Chinese medicine composition；Compound concentration is 106~108The composite bacteria liquid of cfu/mL, pH value are adjusted to 6.5~7.5.
3. fermentation: according to the ratio mixing composite bacteria liquid, Chinese medicine composition and water, sent out in 28 DEG C, the shaking table of 100r/min
Ferment 36 hours, obtain fermentation liquid.
4. purification: by gained filtering fermentation liquor, filtrate inactivates strain in 120 DEG C of at a temperature of high pressure sterilization 30min.
Ferment filtrate after sterilizing is centrifuged 30min under conditions of 8000r/min, centrifugation radius are 9cm, precipitating is abandoned, collects supernatant
Liquid is concentrated with film, concentrate macroporous resin adsorption, then is repeatedly eluted with the ethyl alcohol that volume fraction is 10~95%, and collection is washed
De- liquid, film are concentrated into the concentrate with Chinese medicine identical weight, add and dissolve with the butanediol of concentrate identical weight, filtering,
Chinese medicine composition fermentation liquid (III) as provided by the invention.
Wherein, the amount ratio of composite bacteria liquid, Chinese medicine composition and water is 25mL:30g:300g.
The preparation of 1~5 single medicinal material fermentation liquid of comparative example
Chinese medicine material sophora bud 100g, ginkgo leaf 100g, wild chrysanthemum 100g, achene of Siberian cocklebur 100g and Radix Glycyrrhizae 100g are weighed respectively.
Fermentation extraction is carried out according to preparation method described in embodiment 1, single medicinal material fermentation liquid is successively made respectively.
6 traditional Chinese medicine composition extract liquid of comparative example
According to the consistent Chinese medicine composition dosage of embodiment 1, i.e., Chinese medicine composition is by the Chinese medicine including following weight
Component is made: 28 parts of the sophora bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum, 18 parts of the achene of Siberian cocklebur, 17 parts of Radix Glycyrrhizae.
Preparation method: by the removal of impurities of each Chinese medicine material, cleaning, drying, being crushed to 100 mesh, is 8:1 addition by liquid material mass ratio
Deionized water after impregnating 2.5h, carries out room temperature extraction with high pressure homogenization method machine in the case where 60MPa extracts pressure, extracting solution through from
It after heart separation, is concentrated with film, concentrate macroporous resin adsorption, then is repeatedly eluted with the ethyl alcohol that volume fraction is 10~95%,
Eluent is collected, film is concentrated into extracting solution 100g, adds the dissolution of 100g butanediol, and traditional Chinese medicine composition extract liquid is made in filtering.
Test example 1 inhibits propionibacterium acnes efficacy assessments
1, the preparation of Chinese medical extract sample
A kind of have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution and comparative example 1 for prepared by embodiment 1
~5 preparation single medicinal material fermentation liquid, comparative example 6 prepare traditional Chinese medicine composition extract liquid be formulated as mass fraction be 0.2%,
0.5%, 1.0%, 1.5%, 2.0% sample aqueous solution, it is spare.
2, to the test method of propionibacterium acnes rejection ability
It respectively takes 7 kinds of sample aqueous solution 1mL to be added separately in sterile glass test tube, topples over the previously prepared improvement of 8mL respectively
GAM broth bouillon (Japanese Nissui, number ZZM-05433).Taking-up has divided pure and in 35 DEG C of constant temperature CO2Incubator culture
The clinical propionibacterium acnes of 48h take the injection of 1mL bacteria suspension that the improvement GAM broth bouillon of sample aqueous solution has been added to try respectively
Guan Zhong does negative control with a teat glass without bacteria suspension.Every kind of sample culturing base successively connects since low concentration
Kind propionibacterium acnes, are placed in 35 DEG C of constant temperature CO as early as possible after inoculation2Incubator culture 48h.After culture, improvement GAM meat is first extracted
Liquid in soup culture medium carries out preliminary identification experiment, then is reflected by VITEK full automatic microorganism analysis system, ANI anaerobic bacteria
It is fixed to block the killing situation for determining propionibacterium acnes, as a result as shown in table 1 below.
The killing situation of 1 propionibacterium acnes of table
A kind of has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution to Cuo the result shows that prepared by the present invention
Sore Propionibacterium has obvious inhibitory effect, and the synergistic effect between composition is significant, obviously high to propionibacterium acnes rejection ability
In single medicinal material fermentation liquid prepared by comparative example 1~5.In addition, the fungistatic effect of embodiment 1 is substantially better than comparative example 6, in explanation
After everfermentation, bacteriostasis efficacy is remarkably reinforced drug composition.
Test example 2 removes free radical effect assessment
1, the preparation of sample
Single medicinal material fermentation liquid, right prepared by the Chinese medicine composition fermentation liquid of Examples 1 to 3 preparation and comparative example 1~5
Traditional Chinese medicine composition extract liquid prepared by ratio 6 is made into the sample aqueous solution that mass fraction degree of being is 1%, carries out experiment below.
2, ultra-oxygen anion free radical Scavenging activity is evaluated
The Tris-HCl buffer 4.5mL for taking 0.05mol/L pH=8.2, preheats 20min in 25 DEG C of water-baths.Again plus
Enter 1mL sample aqueous solution and the pyrogallol solution of 0.4mL 25mmol/L, after mixing, reacts 5min in 25 DEG C of water-baths, add
The HCl 1.0mL for entering 8mol/L terminates reaction.Make reference with Tris-HCl buffer, absorbance value is surveyed at 299nm.Blank pair
Sample is substituted according to the solvent of 1mL sample aqueous solution.Clearance rate (D) is calculated according to formula (2).
Ultra-oxygen anion free radical clearance rate (%)=× 100% (2) [1- (A2/A1)]
A1 is the absorbance value of blank control in formula；A2 is the absorbance value of drug.
3, the Scavenging activity of hydroxy radical is evaluated
2mmol/L FeSO is sequentially added in 25mL colorimetric cylinder43mL, 1mmol/L H2O23mL shakes up, and is subsequently added into
6mmol/L salicylic acid 3mL, shakes up, and takes out after 37 DEG C of heating water bath 15min, surveys its absorbance；It is separately added into certain density
Prepare liquid shakes up, and continues heating water bath 15min, takes out and surveys its absorbance.Following formula is removing of the prepare liquid to hydroxy radical (OH)
Rate:
Scavenging action to hydroxyl free radical (%)=[A1-A2- (A1-A3)]/A1 × 100% (3)
A1 is the absorbance value of reaction system before non-dosing product in formula；A2 is the absorbance that drug removes system after OH
Value；A3 is the absorbance value that system after OH is removed in blank control.
4, experimental result
Anti-senescence function evaluation experimental is as shown in table 2.
2 anti-senescence function evaluation experimental result of table
| Sample | To ultra-oxygen anion free radical clearance rate, % | To Scavenging action to hydroxyl free radical, % |
| Embodiment 1 | 95.6 | 92.7 |
| Embodiment 2 | 93.8 | 91.2 |
| Embodiment 3 | 94.6 | 90.7 |
| Comparative example 1 | 89.3 | 82.9 |
| Comparative example 2 | 88.6 | 84.2 |
| Comparative example 3 | 82.6 | 83.4 |
| Comparative example 4 | 62.4 | 42.6 |
| Comparative example 5 | 82.7 | 83.9 |
| Comparative example 6 | 84.2 | 82.3 |
Examples 1 to 3 shows that Chinese medicine composition fermentation liquid of the invention can be potent with the comparison of 1~5 experimental result of comparative example
Free radical is removed, there is good anti-senescence function；And certainly than the removing of single medicinal material fermentation liquid made from comparative example 1~5
It is good by base effect, illustrate to play synergistic effect after optimum organization.Examples 1 to 3 and 6 experimental result of comparative example
Comparison shows that after everfermentation, the effect of the removing free radical of Chinese medicine composition is been significantly enhanced.
3 antiallergic effect assessment of test example
Antiallergic effect is evaluated using the inhibition test to hyaluronidase.Hyaluronidase (commercially available) is hyaluronic acid
Hydrolase, there is close to contact for its generation with inflammation, allergy.By measurement plant antiallergic moisture-keeping composition to hyalomitome
The inhibiting rate of sour enzyme can determine indirectly plant antiallergic moisture-keeping composition to the inhibiting effect of skin allergy.
Testing program: using Elson-Morgan improved method extract to the inhibiting effect test side of hyaluronidase
Method carries out.Take 0.1mL 0.25mmol/L CaCl237 DEG C of heat insulating culture 20min of solution and 0.5mL hyaluronic acid enzyme solution；Respectively
Fermentation liquid or extracting solution 0,0.5,1.0,1.5,2.0mL prepared by embodiment 1 and comparative example 1~6 is added, continues 37 DEG C of heat preservation trainings
Support 20min；0.5mL Sodium Hyaluronate liquid 37 DEG C of heat preservations 30min, room temperature 5min are added；0.1mL 0.4mol/L is added
NaOH solution and 0.5mL acetylacetone,2,4-pentanedione solution are placed in boiling water bath after heating 15min and wake up with a start cooling 5min with ice water immediately；Add
Enter Ehrlich's reagent 1mL, and be diluted with dehydrated alcohol to 5mL, place 20min colour developing, with spectrophotometric determination its
Absorbance value.
Extract is calculated as follows to the inhibiting rate of hyaluronidase:
Inhibiting rate (%)=[(A-B)-(C-D)]/(A-B) × 100%
In formula, A is the absorbance of contrast solution (Sodium Hyaluronate+hyaluronidase+deionized water)；B is control blank
The absorbance of solution (hac buffer+deionized water)；C is that (potassium hyaluronate+hyaluronidase+sample is molten for sample solution
Liquid) absorbance；D is the absorbance of sample blank solution (hac buffer+sample solution).
Test result is shown in Table 3.
Result of the table 3 to the inhibiting rate of hyaluronidase
Examples 1 to 3 shows that Chinese medicine composition fermentation liquid of the invention can be potent with the comparison of 1~5 experimental result of comparative example
Inhibit hyaluronidase, has the function of good antiallergic；And than the inhibition of single medicinal material fermentation liquid made from comparative example 1~5
Hyaluronic acid enzyme effect is good, illustrates to play synergistic effect after optimum organization.Examples 1 to 3 and comparative example 6 are real
It tests Comparative result to show after everfermentation, the effect of the inhibition hyaluronidase of Chinese medicine composition is been significantly enhanced.
4 irritation evaluation experimental of test example
Fermentation liquid irritation is evaluated using rabbit erythrocyte hemolytic experiment.Concrete operations are as follows: taking 1~6 He of comparative example respectively
The fermentation liquid or 500 μ L of extracting solution of Examples 1 to 3 preparation are placed in 2mL centrifuge tube, then are separately added into 2% rabbit erythrocyte (city
Sell) 500 μ L of suspension, jog mixes, is placed in 37 DEG C of constant incubators after incubating 3h, and 2000r/min is centrifuged 3min, draws supernatant
Liquid, using distilled water as positive control, light absorption value is measured at wavelength 414nm, is calculated by formula using physiological saline as negative control
Haemolysis degree.The results are shown in Table 4.
4 haemolysis degree result of table
Examples 1 to 3 shows Chinese medicine composition fermentation liquid haemolysis ratio of the invention with the comparison of 1~5 experimental result of comparative example
Example is more much lower than single medicinal material fermentation liquid made from comparative example 1~5, and irritation is much smaller.Examples 1 to 3 and comparative example 6 are tested
Comparative result shows that after everfermentation, the irritation of Chinese medicine composition fermentation liquid is significantly reduced.
1 antipollution facial mask of Application Example
A kind of antipollution face pack prescription of the Chinese medicine composition fermentation liquid of effect containing antipollution is shown in Table 5.
5 antipollution face pack prescription of table
| Component | Mass fraction/% |
| Chinese medicine composition fermentation liquid (I) of the invention | 5 |
| Glycerol | 6.00 |
| 1,3- propylene glycol | 4.00 |
| Sodium Hyaluronate | 0.10 |
| Xanthan gum | 0.10 |
| Hydroxyethyl cellulose | 0.10 |
| Dipotassium glycyrrhizinate | 0.50 |
| Aloe extract | 1.00 |
| 1,2- hexylene glycol | 0.80 |
| Parahydroxyacet-ophenone | 0.50 |
| Deionized water | Surplus |
The preparation method is as follows:
(1) xanthan gum and Sodium Hyaluronate are dispersed in advance in glycerol, then with dipotassium glycyrrhizinate, hydroxy ethyl fiber
Element, Chinese medicine composition fermentation liquid (I) are put into deionized water together, and stirring and dissolving is transparent to being uniformly dispersed；
(2) parahydroxyacet-ophenone is dissolved by heating in 1,3-PD and 1,2- hexylene glycol in advance, investment to step (1)
In the solution, stirring and dissolving is uniform；
(3) aloe extract is put into again, is evenly stirred until and is completely dissolved；
(4) color, pH, fragrance, the physical and chemical indexes such as appearance, after the assay was approved 200 mesh filtering and discharging are examined.
2 antipollution face cream of Application Example
Table 6 provides the antipollution prescription for skin cream containing Chinese medicine composition fermentation liquid of the invention.
6 antipollution prescription for skin cream of table
| Ingredient | Mass fraction (%) |
| Arachidic alcohol glucoside | 1.5 |
| PEG-100 stearate | 1.2 |
| Stearine | 1 |
| Cetearyl glucoside | 0.8 |
| Shea butter | 0.5 |
| White pond flower seed oil | 3 |
| Parleam | 3 |
| Cyclomethicone | 4 |
| Dimethyl silicone polymer | 2 |
| Methyl hydroxybenzoate | 0.2 |
| Nipasol | 0.08 |
| EDTA‐2Na | 0.02 |
| Glycerol | 5 |
| 1,3 butylene glycol | 3 |
| Sodium Hyaluronate | 0.05 |
| Carbomer (CA) | 0.2 |
| Phenoxyethanol | 0.3 |
| Aminomethyl propanol | 0.12 |
| Essence | 0.1 |
| Chinese medicine composition fermentation liquid (I) of the invention | 5 |
| Deionized water | Surplus |
Preparation method:
(1) 1,3-BDO and Sodium Hyaluronate are evenly stirred until in advance it is transparent, carbomer be made into mass fraction be 1%
Aqueous dispersions；
(2) by Oil soluble materials (arachidic alcohol glucoside, PEG-100 stearate, stearine, cetearyl
Glucoside, shea butter, white pond flower seed oil, Parleam, cyclomethicone, dimethyl silicone polymer, oxybenzene
Methyl esters, Nipasol) it puts into oily phase pot and is heated to 80 DEG C, stirring is to dissolving completely, for oily phase；
(3) will through 1,3 butylene glycol that step (1) is handled well, Sodium Hyaluronate, Chinese medicine composition fermentation liquid (I) and other
Aqueous Phase Raw Material (EDTA-2Na, glycerol, aminomethyl propanol) investment emulsion pot is heated to 80 DEG C, and stirring is water phase to dissolving completely；
(4) it is mutually quickly pumped into oily into the emulsion pot of step (3), after addition, with the rate of 4000~6000rpm
It emulsifying 5~10 minutes, is persistently stirred after emulsification with the rate of 1000~2000rpm and starts to cool down；
(5) when being down to 65 DEG C, the carbomer aqueous dispersions for being 1% through step (1) prepared mass fraction are added, with
The rate homogeneous of 4000~6000rpm 2 minutes；
(6) 45 DEG C being cooled to temperature, being added essence, Phenoxyethanol, stirring is to being uniformly dissolved；
(7) vacuum defoamation after temperature is down to 35 DEG C, inspection color, fragrance, the physical and chemical indexes such as appearance, after the assay was approved
200 mesh filtering and dischargings.
The common facial mask of Comparison study example 1
In addition to without other than Chinese medicine composition fermentation liquid (I) of the invention, other formula components, dosage, preparation method and answer
It is consistent with embodiment 1.
1 anti-acne effect of effect example, irritation evaluation and human skin photoelastic evaluation
Evaluation object: selecting face to have acne, age is young man 60 of 15~30 years old, wherein male 30, women
30, stochastic averagina is divided into A group and two groups of B group, every group of 30 people.
Evaluation method: using double-blind study, and A group uses the antipollution facial mask of the preparation of Application Example 1 sooner or later daily, and B group is daily
Sooner or later the common facial mask prepared with Comparison study example 1；Massage face is until facial mask liquid is all absorbed by the skin after removing facial mask.
Evaluate the course for the treatment of: anti-acne effect evaluation is evaluated with 20 days as a treatment course, irritation with 20 days as a treatment course, human body
Skin elasticity evaluation is as a treatment course with 7 weeks.
Anti-acne effect evaluation criterion: 1. effective: 70% or more acne fading；2. effectively: 30% or more acne fading；3. nothing
Effect: 30% or less acne fading.
Irritation evaluation criterion: it is checked according to 7 standard of table.It has carried out testing for 20 days altogether.
7 irritation evaluation criterion of table
| Grade | Standard of perfection |
| 0 | Negative reaction: non-stimulated, without erythema |
| 1 | Suspicious reaction: slight erythema |
| 2 | Weakly positive reaction: erythema |
| 3 | Strong positive reaction: erythema, papule, blister |
| 4 | Extremely strong positive reaction: serious edema, bulla |
Human skin photoelastic evaluation standard: skin elasticity tester the 2nd, 4,6 week progress skin after applying facial mask is used
Flexibility test calculates the average increment rate of each group volunteer skin elasticity.
Anti-acne effect evaluation result: antipollution facial mask effective 6 of the preparation of Application Example 1 account for 20%；Effective 15,
Account for 50%；No effect 9 accounts for 30%.Total effective rate reaches 70%；Common facial mask effective 0 of the preparation of Comparison study example 1, effective 2
Example, invalid 28, efficient only 6.7%.Illustrate that the traditional chinese medicine composition of the invention fermentation liquid has good anti-acne effect.
Irritation evaluation result: as shown in table 8.
8 irritation evaluation result of table
| Allergy grade | Comparison study example 1 | Application Example 1 |
| 0 grade number | 25 | 30 |
| 1 grade number | 4 | 0 |
| 2 grade numbers | 1 | 0 |
| 3 grade numbers | 0 | 0 |
| 4 grade numbers | 0 | 0 |
As seen from the results in Table 8, the common of the preparation of Comparison study example 1 of Chinese medicine composition fermentation liquid of the invention is not added
There are allergic phenomenas for facial mask, and antipollution face prepared by the Application Example 1 for being added to Chinese medicine composition fermentation liquid of the invention
There is not allergic phenomena in film, has effects that antiallergic.
Human skin photoelastic evaluation result: as shown in table 9.
9 skin elasticity test result of table
As shown in Table 9, the antipollution prepared using the Application Example 1 for being added to Chinese medicine composition fermentation liquid of the invention
The skin elasticity increment rate of volunteer does not add the Comparison study of Chinese medicine composition fermentation liquid of the invention than using after facial mask
Common facial mask prepared by example 1 is obviously improved, and has good anti-aging effects.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, it is characterised in that: described is used for cosmetic
Chinese medicine composition fermentation liquid is by including that the traditional Chinese medicinal components of following parts by weight are made: 10~40 parts of the sophora bud, 5~30 parts of ginkgo leaf,
5~30 parts of wild chrysanthemum, 5~30 parts of the achene of Siberian cocklebur, 5~30 parts of Radix Glycyrrhizae.
2. according to claim 1 have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, it is characterised in that:
Described has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, by the Chinese medicine group including following parts by weight
Divide and is made: 25~30 parts of the sophora bud, 15~20 parts of ginkgo leaf, 17~20 parts of wild chrysanthemum, 18~20 parts of the achene of Siberian cocklebur, Radix Glycyrrhizae 10~20
Part.
3. according to claim 1 or 2 have effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution, feature exists
In:
Compound bacteria used in the fermentation is lactobacillus plantarum and S. cervisiae；
Lactobacillus plantarum and S. cervisiae be in proportion (1~5) in the compound bacteria: 1 composition, and composite bacteria liquid concentration is 106~
108cfu/mL。
4. the described in any item preparations with the Chinese medicine composition fermentation liquid used for cosmetic of antipollution of claims 1 to 3
Method, it is characterised in that:
Using fermentation extraction method, i.e., after Chinese medicine composition being carried out fermentation process, obtained using filter, sterilizing and centrifugal separation
Chinese medicine composition fermentation liquid；
Specifically comprise the following steps:
1. compound bacteria is added in the form of bacterium solution, the composite bacteria liquid be lactobacillus plantarum and S. cervisiae in proportion (1~
5): 1 composition, composite bacteria liquid concentration are 106~108cfu/mL；
2. stock: by the removal of impurities of each Chinese medicine material, cleaning, drying, being crushed to 80~200 mesh, mix, obtain Chinese medicine composition；Match
Concentration processed is 106~108The composite bacteria liquid of cfu/mL, pH value are adjusted to 6.5~7.5；
3. fermentation: according to the ratio mixing composite bacteria liquid, Chinese medicine composition and water, in 28~42 DEG C, 50~300r/min condition
Fermentation 36~48 hours, obtains fermentation liquid；
4. purification: by gained filtering fermentation liquor, filtrate makes strain in 105~120 DEG C of at a temperature of 20~35min of high pressure sterilization
Inactivation；By the ferment filtrate after sterilizing 3000~8000r/min, centrifugation radius be 5~20cm under conditions of centrifugation 20~
35min, abandon precipitating, collect supernatant, be concentrated with film, concentrate macroporous resin adsorption, then with volume fraction be 10~95%
Ethyl alcohol repeatedly elute, collect eluent, film is concentrated into the concentrate with Chinese medicine composition identical weight, adds and concentrate
The butanediol of identical weight dissolves, and filtering as has effects that the Chinese medicine composition fermentation liquid used for cosmetic of antipollution.
5. the preparation method with the Chinese medicine composition fermentation liquid used for cosmetic of antipollution according to claim 4,
It is characterized by:
The amount ratio of the composite bacteria liquid, Chinese medicine composition and water is 20mL~60mL:20g~60g:200~600g；
Step 1. described in composite bacteria liquid be that 3:1 is formed in proportion for lactobacillus plantarum and S. cervisiae；
Step 3. in, ferment 36~48 hours in 28~35 DEG C, 100r/min condition, obtain fermentation liquid.
6. prepared by the described in any item Chinese medicine composition fermentation liquids used for cosmetic with antipollution of claims 1 to 3
Application in cosmetics.
7. application according to claim 6, it is characterised in that:
Described has effects that additive amount of the Chinese medicine composition fermentation liquid used for cosmetic of antipollution in cosmetics with quality point
Number is calculated as 0.1~15%.
8. a kind of cosmetics, it is characterised in that: have effects that the makeup of antipollution containing claims 1 to 3 is described in any item
Product Chinese medicine composition fermentation liquid.
9. cosmetics according to claim 8, it is characterised in that:
The cosmetics, which contain, has effects that the Chinese medicine composition used for cosmetic of antipollution based on mass fraction for 0.1~15%
Fermentation liquid；
The cosmetics are as follows: aqua, type of latex type, cream kind, gel class, powder class, powder compacting class, facial mask, spray-like, essence
Class washes shield class.
10. cosmetics according to claim 8 or claim 9, it is characterised in that:
The cosmetics be antipollution facial mask, the component including following mass fraction meter:
The cosmetics be antipollution face cream, the component including following mass fraction meter:
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| CN108904376A (en) * | 2018-08-02 | 2018-11-30 | 广东轻工职业技术学院 | It is a kind of to have effects that the Chinese medicine compound prescription fermentation liquid used for cosmetic of oil control and acne removal and its preparation and application |
| CN111481492A (en) * | 2020-06-12 | 2020-08-04 | 广州鹰远生物科技有限公司 | Composition containing traditional Chinese medicine compound extract and application thereof |
| CN114831908A (en) * | 2022-04-28 | 2022-08-02 | 江苏农牧科技职业学院 | Ginkgo episperm anti-aging acne-removing cream and preparation method thereof |
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