CN109055277B - 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 - Google Patents
一种从土壤中筛选嗜酸耐重金属固氮菌的方法 Download PDFInfo
- Publication number
- CN109055277B CN109055277B CN201811081231.4A CN201811081231A CN109055277B CN 109055277 B CN109055277 B CN 109055277B CN 201811081231 A CN201811081231 A CN 201811081231A CN 109055277 B CN109055277 B CN 109055277B
- Authority
- CN
- China
- Prior art keywords
- culture
- strain
- acidophilic
- heavy metal
- azotobacter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 56
- 239000002689 soil Substances 0.000 title claims abstract description 56
- 241000589151 Azotobacter Species 0.000 title claims abstract description 46
- 238000012216 screening Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 52
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 230000002378 acidificating effect Effects 0.000 claims abstract description 27
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 239000002253 acid Substances 0.000 claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 15
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 12
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000011248 coating agent Substances 0.000 claims abstract description 7
- 238000000576 coating method Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 18
- 239000002244 precipitate Substances 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 11
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 229910018890 NaMoO4 Inorganic materials 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 9
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 229910052603 melanterite Inorganic materials 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 9
- 239000011686 zinc sulphate Substances 0.000 claims description 9
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229910017009 AsCl3 Inorganic materials 0.000 claims description 4
- 229910021556 Chromium(III) chloride Inorganic materials 0.000 claims description 4
- 108010010803 Gelatin Proteins 0.000 claims description 4
- HDSBZMRLPLPFLQ-UHFFFAOYSA-N Propylene glycol alginate Chemical compound OC1C(O)C(OC)OC(C(O)=O)C1OC1C(O)C(O)C(C)C(C(=O)OCC(C)O)O1 HDSBZMRLPLPFLQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000011636 chromium(III) chloride Substances 0.000 claims description 4
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 4
- 229910052564 epsomite Inorganic materials 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 235000010409 propane-1,2-diol alginate Nutrition 0.000 claims description 4
- 239000000770 propane-1,2-diol alginate Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 abstract description 9
- 238000000746 purification Methods 0.000 abstract description 3
- 239000002609 medium Substances 0.000 description 9
- 230000020477 pH reduction Effects 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811081231.4A CN109055277B (zh) | 2018-09-17 | 2018-09-17 | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811081231.4A CN109055277B (zh) | 2018-09-17 | 2018-09-17 | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109055277A CN109055277A (zh) | 2018-12-21 |
CN109055277B true CN109055277B (zh) | 2021-09-24 |
Family
ID=64761953
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811081231.4A Active CN109055277B (zh) | 2018-09-17 | 2018-09-17 | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109055277B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112745860A (zh) * | 2021-02-23 | 2021-05-04 | 潍坊科技学院 | 一种基于石墨烯的酸化土壤调理剂 |
CN113337419B (zh) * | 2021-04-26 | 2023-06-13 | 沈阳华清源农业发展有限公司 | 一种土壤酸化地区用微生物菌剂的制备方法 |
CN115820493A (zh) * | 2022-12-01 | 2023-03-21 | 安徽农业大学 | 一种固氮菌富集和用纤维素培养固氮菌降解染料的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104862250A (zh) * | 2015-05-08 | 2015-08-26 | 周洪波 | 一种嗜酸铁氧化微生物复合菌剂及其制备方法 |
CN108018250A (zh) * | 2018-01-29 | 2018-05-11 | 武汉新禹智水环保科技有限公司 | 一株嗜酸氧化亚铁硫杆菌及其在环境治理中的应用 |
-
2018
- 2018-09-17 CN CN201811081231.4A patent/CN109055277B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104862250A (zh) * | 2015-05-08 | 2015-08-26 | 周洪波 | 一种嗜酸铁氧化微生物复合菌剂及其制备方法 |
CN108018250A (zh) * | 2018-01-29 | 2018-05-11 | 武汉新禹智水环保科技有限公司 | 一株嗜酸氧化亚铁硫杆菌及其在环境治理中的应用 |
Non-Patent Citations (2)
Title |
---|
Genome-Directed Isolation of the Key Nitrogen Fixer Leptospirillum ferrodiazotrophum sp.nov.froma an Acidophilic Microbial Community;Gene W,et al;《Applied and Environmental Microbiology》;20051031;全文 * |
几种特定生物学功能的微生物的筛选与鉴定;王婧;《中国优秀硕士学位论文全文数据库》;20130215;全文 * |
Also Published As
Publication number | Publication date |
---|---|
CN109055277A (zh) | 2018-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109055277B (zh) | 一种从土壤中筛选嗜酸耐重金属固氮菌的方法 | |
CN105713862B (zh) | 可降解吡啶和氨氮的菌株及其应用 | |
CN108220169B (zh) | 一种降解聚苯乙烯菌种的分离筛选及其鉴定方法 | |
CN110904004B (zh) | 一株产海藻糖水解酶的细菌及其选育方法和应用 | |
CN106754481B (zh) | 从冰菜中筛选耐高盐固氮菌株的方法及相关培养基 | |
CN103540544A (zh) | 一株可降解吡啶的根瘤杆菌、选育方法及其应用 | |
US20240060153A1 (en) | Acidomyces Acidothermus and Its Application in Leaching Copper-containing Pollutants from Waste Circuit Boards | |
CN107937302B (zh) | 一种提高重金属有效性的细菌d10及其应用 | |
CN107384804B (zh) | 赤霉菌nt-1及其应用 | |
CN110343645B (zh) | 一株菌株短小芽孢杆菌sem-7及其应用 | |
CN109536392B (zh) | 杂色曲霉zjb16085及合成r-2-(4-羟基苯氧基)丙酸的应用 | |
CN117417838A (zh) | 一种好氧反硝化真菌共培养物、制备方法及其应用 | |
CN111849844A (zh) | 一种地衣芽孢杆菌a10201及其应用 | |
CN115305226B (zh) | 一株降解烟碱并产氢的抗辐射不动杆菌zj-22及其应用 | |
CN115141770B (zh) | 一种能够降解畜禽废水中cod的特基拉芽孢杆菌h1及其应用 | |
CN116478862A (zh) | 一株降解黄曲霉毒素的菌株、制剂及降解黄曲霉毒素的方法 | |
CN114410508B (zh) | 一种油脂降解菌及其筛选方法与应用 | |
CN104694435A (zh) | 一株具有三氮唑降解功能的申氏杆菌及其应用 | |
CN109880752B (zh) | 一种异养硝化-好氧反硝化菌及其鉴定方法 | |
CN105670965B (zh) | 一种具有铁还原能力的菌种及其应用 | |
CN110042063B (zh) | 一种粉红粘帚霉(Clonostachys rosea)菌株YZC3及其应用 | |
CN109749959B (zh) | 一种具有固氮活性的菌株hb161398及其应用 | |
CN110669716A (zh) | 一种耐高浓度苯酚、重金属和耐低温红球菌分离方法 | |
CN111254102A (zh) | 柠檬明串珠菌及其在沉淀淀粉浆液中的应用 | |
CN113862185B (zh) | 一种耐盐节杆菌及其固氮应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: 230000 No.20 Tongling Road, Yaohai District, Hefei City, Anhui Province Patentee after: Anhui Construction Engineering Transportation and Navigation Group Co.,Ltd. Patentee after: ANHUI University Patentee after: ANHUI CONSTRUCTION ENGINEERING GROUP HOLDINGS Co.,Ltd. Address before: 230011 No. 20, Tongling Road, Hefei, Anhui Patentee before: ANHUI PROVINCE TRANSPORTATION HARBOR ENGINEERING CO.,LTD. Patentee before: ANHUI University Patentee before: ANHUI CONSTRUCTION ENGINEERING GROUP HOLDINGS Co.,Ltd. |