CN109053239A - A kind of preparation method of mosquito eradication potting soil - Google Patents
A kind of preparation method of mosquito eradication potting soil Download PDFInfo
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- CN109053239A CN109053239A CN201810900204.9A CN201810900204A CN109053239A CN 109053239 A CN109053239 A CN 109053239A CN 201810900204 A CN201810900204 A CN 201810900204A CN 109053239 A CN109053239 A CN 109053239A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses a kind of preparation methods of mosquito eradication potting soil, belong to mosquito eradication applied technical field.For the present invention with urea and a variety of bacterium etc. for raw material, the bacteria agent processed of fermenting attracts mosquito in conjunction with other active constituents, then kills mosquito, finally decomposes the intracorporal tissue of mosquito and is nutrient and kills germ entrained by it, reduces the propagation of disease;Bentonite is modified, poly- lysine is cooperated to effectively improve the anti-microbial property of system, pass through mosquito by excitement, spasm, paralysis to dead process, reach effect exterminating mosquito, the albumen that a variety of pairs of mosquito bodies have decomposition is extracted by raw material of long octopus salivary gland, it is translated into simple nutrient, lures mosquito ingredient to achieve the effect that compound to lure mosquito so that the system such as caustic soda, pigskin grease is compound.The present invention solves the problems, such as that currently used mosquito killing liquid function and effect are single, effect exterminating mosquito is bad.
Description
Technical field
The invention belongs to mosquito eradication applied technical fields, and in particular to a kind of preparation method of mosquito eradication potting soil.
Background technique
Mosquito belongs to Insecta Diptera Dulicidae, and there are about 3000 kinds in the whole world.The female mosquito sucked blood is dengue fever, malaria, Huang
The vector of the other pathogens such as pyreticosis, filariasis, encephalitis B.Mosquito it is main harm be spread the disease, it was found that
The disease of mosquitoes spread is up to as many as more than 80 kinds.There are about 700,000,000 people every year infects various diseases by mosquito, and in every 17 people, just has
1 people dies of the various diseases infected by mosquito.Especially in torrid areas, multiple infectious disease is often transmitted to the mankind by mosquito.
Mainly prevent mosquito close to human body using pest repellant in daily life.Existing mosquito repellent class product mosquito repellent
Water is using artificial synthesized chemicals Metadelphene as the product of primary raw material.Metadelphene is a kind of widely used insecticide, is changed
Studying point has certain toxic side effect to human body, is even more especially that can not look down upon to the harmfulness of children and the elderly.Therefore
It is necessary to develop the good and more environmentally-friendly mosquito repellent product of repelling effect.Mosquito-repellent water liquid product is many kinds of currently on the market, but
It is that function and effect are single, effect exterminating mosquito is bad.
Summary of the invention
The technical problems to be solved by the invention: for currently used mosquito killing liquid function and effect are single, effect exterminating mosquito not
Good problem provides a kind of preparation method of mosquito eradication potting soil.
In order to solve the above technical problems, the present invention is using technical solution as described below:
A kind of preparation method of mosquito eradication potting soil includes the following steps:
(1) according to parts by weight, successively take 30 ~ 50 parts of stalks, 20 ~ 30 parts of wormcasts, 15 ~ 30 parts of duck excrements, 20 ~ 40 parts of urea,
20 ~ 35 parts of bacteria agents, 50 ~ 75 parts of water are mixed, and are protected from light ventilation and stack, obtain stack retting material, take heap in mass ratio for 1:6 ~ 10
Material and water mixing are macerated, boiling is heated, filters while hot, obtain excrement corrosive liquid, it is spare;
(2) 6 ~ 10:1 in mass ratio takes sodium bentonite, auxiliary agent to pulverize and sieve, and take sieving particle is that 1:3 ~ 6:5 adds by volume
Entering HCl solution, sodium citrate solution mixing, constant temperature is stirred to react, filters, obtain filter cake, filter cake is taken to be washed with water, and it is dry, it obtains sour
It soaks bentonite and takes acidleach bentonite to mix with solvent A for 1:30 ~ 50 in mass ratio in 55 ~ 60 DEG C, constant temperature stirring, filtering obtains
Filter residue takes filter residue to wash, dry, obtains modified alta-mud, spare;
(3) glandula digestive of fresh long octopus is taken, is rinsed, homogenate obtains slurry, takes slurry 1:10 in mass ratio ~ 15 that solvent B mixing is added
Stirring, obtains mixed liquor, takes mixed liquor 7 ~ 12:1 in mass ratio that ammonium sulfate is added, saltouts, and dialyses, centrifugation, and supernatant is taken to be added
The ammonium sulfate of clear liquid quality 30 ~ 50%, saltouts, and dialyses, centrifugation, takes centrifugation object refrigeration, obtains active matter, take active matter in mass ratio
Essential oil, the mixing of Tris-HCl buffer is added in 1:4 ~ 7:15, is evaporated in vacuo, obtains concentrate, spare;
(4) 1:10 ~ 15:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 75 ~ 90 DEG C, heat preservation is cold
But, saponification grease is obtained, in 35 ~ 50 DEG C, 5:1:1 ~ 2:20 ~ 30 take saponification grease, sodium metasilicate, detergent alkylate sulphur in mass ratio
Sour sodium, water are mixed, and obtain premixed liquid, and in 55 ~ 70 DEG C, 6:0.1 ~ 0.3:1 in mass ratio ~ 3:0.1 takes premixed liquid, adjuvant, pre-
Handle rice washing water, sizing material is mixed, ultrasonication obtains and compound lures mosquito ingredient;
(5) according to the mass fraction, take 30 ~ 50 parts of garden soil, 20 ~ 40 parts of clays, 15 ~ 30 parts of activated sludge, 1 ~ 4 part of fragrance, 5 ~
10 parts of allethrins, 8 ~ 15 parts of ES Esbiothrins, 6 ~ 10 parts of poly- lysines, 20 ~ 40 parts it is compound lure mosquito ingredient, 10 ~ 20 parts it is standby
Excrement corrosive liquid, 15 ~ 25 parts of spare modified alta-muds, 15 ~ 30 portions of spare concentrates, 20 ~ 40 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, the 20 ~ 30% of premix soil property amount is taken to rely with poly-
Propylhomoserin, it is compound lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, the mixing of spare concentrate, obtain upper layer
Soil takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin,
Up to mosquito eradication potting soil.
Stalk in the step (1): wheat stalk, cotton stalk, any one in rice straw.
Bacteria agent in the step (1): according to the mass fraction, 2 ~ 5 parts of thermophilic lactobacillis, 4 ~ 8 parts of plant cream bars are taken
Bacterium, 2 ~ 5 parts of photosynthetic bacterias, 8 ~ 15 parts of Bacillus pasteuriis, 5 ~ 10 parts of molasses, 15 ~ 25 parts of yeast extracts, 1 ~ 4 part of sodium chloride, 0.2 ~
0.6 part of sodium nitrite, 0.4 ~ 0.8 part of calcium chloride, 800 ~ 1000 parts of sterile water mixing, shaking table culture are sealed by fermentation to get biology
Microbial inoculum.
Auxiliary agent in the step (2): 5:1 ~ 2 take potassium humate, sodium citrate mixing to get auxiliary agent in mass ratio.
Solvent A in the step (2): 12 ~ 16:1 in mass ratio takes silver nitrate solution, sodium secondary alkyl sulfonate mixing, i.e.,
Obtain solvent A.
Solvent B: 12 ~ 18:1 in mass ratio in the step (3) takes PBS buffer solution, NaCl mixing to get solvent B.
Essential oil in the step (3): 5:1 ~ 2 take wintersweet flower essential oil and Lavender to mix to get essence in mass ratio
Oil.
Adjuvant in the step (4): 1:1 ~ 3:1:12 ~ 18 take cellulose, sodium alkyl sulfate, cocounut oil in mass ratio
Alkyl oxalamide, glycerol mixing are to get adjuvant.
Pretreatment rice washing water in the step (4): taking fresh rice washing water sealed light-transmitting solarization, discharges, and filtering takes filter
Liquid vacuum concentration, obtains concentrate, and 5 ~ 8:1 in mass ratio takes concentrate and ammonium carbonate to mix to get pretreatment rice washing water.
Sizing material in the step (4): lac, peach gum, any one in xanthan gum.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention is with urea and a variety of bacterium etc. for raw material, and ferment bacteria agent processed, in stack retting effect, heating process, high fertile processed
Imitate excrement corrosive liquid, can produce a large amount of carbon dioxide, can lower jaw antenna to mosquito arrange unique sensory cell and formed by force
Strong stimulation, cooperate eight enol ingredients in the essential oil in subsequent step, it is compound lure mosquito ingredient, can pinniform antenna factory to mosquito
Organ generates fascination effect, and in conjunction with other active constituents, the present invention attracts mosquito, then kills mosquito, finally decomposes in mosquito body
Tissue be nutrient and kill germ entrained by it, reduce the propagation of disease;
(2) present invention is modified bentonite, in modifying process, is distributed in bentonite channel using hydrochloric acid solution removal
The acid-soluble and part organic impurities in portion dredges bentonite duct, recycles silver nitrate solution to be modified, keeps low electricity price more than half
Ion exchange occurs for the silver ion and bentonite interlayer cation of diameter, under sodium secondary alkyl sulfonate auxiliary, so that stratiform bentonite
It removes, be dispersed into thinner monolithic, and there is bigger specific surface area, be conducive to the circulation transmitting between each nutrient, on the other hand
The presence of silver ion can cooperate poly- lysine to effectively improve the anti-microbial property of system, in use, can be to mosquito certainly
The germ that body carries carries out killing effect, the interference of its growth to potting can be effectively avoided, along with the biology of soil system
Effect, allethrin, the distillation of ES Esbiothrin ingredient, passes through mosquito by excitement, spasm, paralysis to dead process, reaches
Effect exterminating mosquito extracts the albumen that a variety of pairs of mosquito bodies have decomposition by raw material of long octopus salivary gland, is translated into letter
Monoculture point lures mosquito ingredient so that the system such as caustic soda, pigskin grease is compound, can the sweat odor molecule to human body simulate, and in soil
Under the action of can play slow releasing function to scent molecule again so that smell is thin out, but the strong mosquito of susceptibility is acted on obvious, reached
To the compound effect for luring mosquito, the pretreatment rice washing water itself in ingredient can give soil to provide nutrient abundant, and decomposable mosquito
Daughter lactones insoluble macromolecular substance is the easy absorbing material of small molecule, promotes the growth of potting.
Specific embodiment
Stalk: wheat stalk, cotton stalk, any one in rice straw.
Bacteria agent: according to the mass fraction, 2 ~ 5 parts of thermophilic lactobacillis, 4 ~ 8 parts of lactobacillus plantarums, 2 ~ 5 parts of photosynthetic bacterias, 8 are taken
~ 15 parts of Bacillus pasteuriis, 5 ~ 10 parts of molasses, 15 ~ 25 parts of yeast extracts, 1 ~ 4 part of sodium chloride, 0.2 ~ 0.6 part of sodium nitrite, 0.4 ~
0.8 part of calcium chloride, 800 ~ 1000 parts of sterile water mixing are sealed by fermentation 3 ~ 6 days with 200 ~ 250r/min shaking table culture to get life
Object microbial inoculum.
Solvent A: 12 ~ 16:1 in mass ratio take mass fraction be 10% silver nitrate solution, sodium secondary alkyl sulfonate mixing to get
Solvent A.
Solvent B: 12 ~ 18:1 in mass ratio takes the PBS buffer solution that pH value is 6.7 ~ 7.2, NaCl to mix to get solvent B.
5:1 ~ 2 take wintersweet flower essential oil and Lavender to mix to get essential oil in mass ratio.
Pretreatment rice washing water: taking fresh rice washing water in closing transparent vessel, solarization 3 ~ 5 days, discharges, and filtering takes filtrate
In 55 ~ 70 DEG C, the 35 ~ 50% of original volume are concentrated in vacuo to, obtain concentrate, 5 ~ 8:1 in mass ratio takes concentrate and ammonium carbonate mixed
It closes to get pretreatment rice washing water.
Adjuvant: 1:1 ~ 3:1:12 ~ 18 take cellulose, sodium alkyl sulfate, Cocoalkyl oxalamide, glycerol in mass ratio
Mixing is to get adjuvant.
Auxiliary agent: 5:1 ~ 2 take potassium humate, sodium citrate mixing to get auxiliary agent in mass ratio.Sizing material: lac, peach gum, Huang
Any one in virgin rubber.
A kind of preparation method of mosquito eradication potting soil, includes the following steps:
(1) according to parts by weight, successively take 30 ~ 50 parts of stalks, 20 ~ 30 parts of wormcasts, 15 ~ 30 parts of duck excrements, 20 ~ 40 parts of urea,
20 ~ 35 parts of bacteria agents, 50 ~ 75 parts of water are mixed in container, logical in being protected from light after stirring 20 ~ 30min with 300 ~ 400r/min revolving speed
It is stacked 7 ~ 10 days at wind, obtains stack retting material, take stack retting material and water to mix for 1:6 ~ 10 in mass ratio, in 95 ~ 100 DEG C of heating boilings 30
~ 45min, is filtered while hot, obtains excrement corrosive liquid, spare;
(2) 6 ~ 10:1 in mass ratio takes sodium bentonite, auxiliary agent to crushed 200 meshes in pulverizer, takes sieving particle by volume
Sodium citrate solution than HCl solution, mass fraction 25% for 1:3 ~ 6:5 addition concentration 0.01mol/L is mixed in reaction kettle,
In 45 ~ 55 DEG C, with 400 ~ 600r/min under the conditions of, constant temperature is stirred to react 2 ~ 4h, filtering, obtain filter cake, take filter cake deionized water
After washing is in neutrality to cleaning solution, 70 ~ 90 DEG C of oven dryings are transferred to constant weight, acidleach bentonite are obtained, in 55 ~ 60 DEG C, by quality
Than taking acidleach bentonite to mix with solvent A for 1:30 ~ 50, under the conditions of 300 ~ 500r/min, constant temperature is stirred to react 45 ~ 60min,
Filtering, obtains filter residue, after taking filter residue to be washed with deionized filter residue 3 ~ 5 times, moves to 100 ~ 110 DEG C of oven dryings to constant weight, must change
Property bentonite, it is spare;
(3) glandula digestive for taking fresh long octopus, with water rinse 2 ~ 5 times after, be put into refiner and be homogenized, obtain slurry, take slurry by
Mass ratio 1:10 ~ 15 is added the PBS buffer solution that pH value is 6.7 ~ 7.2 and mixes, with 400 ~ 600r/min, 25 ~ 50min of magnetic agitation,
Mixed liquor, take mixed liquor 7 ~ 12:1 in mass ratio that ammonium sulfate is added, saltout, after 4 ~ 8 DEG C of dialysed overnights, in 1000 ~
2000r/min is centrifuged 10 ~ 15min, takes the ammonium sulfate of supernatant addition supernatant quality 30 ~ 50% is secondary to saltout, thoroughly in 4 ~ 8 DEG C
After analysis, it is centrifuged 10 ~ 15min in 1000 ~ 2000r/min, takes centrifugation object in 1 ~ 4 DEG C of refrigerator cold-storage 20 ~ for 24 hours, obtains active matter, take
The Tris-HCl buffer that wintersweet flower essential oil is added in active matter 1:4 ~ 7:15 in mass ratio, pH is 6.5 ~ 6.9 mixes, in 40 ~ 55
It DEG C is evaporated in vacuo to the 30 ~ 45% of original volume, obtains concentrate, it is spare;
(4) 1:10 ~ 15:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 75 ~ 90 DEG C, and heat preservation 1 ~
3h, natural cooling obtain saponification grease, and in 35 ~ 50 DEG C, 5:1:1 ~ 2:20 ~ 30 take saponification grease, sodium metasilicate, ten in mass ratio
Dialkyl benzene sulfonic acids sodium, water mixing, with 400 ~ 600r/min, 20 ~ 45min of magnetic agitation, obtain premixed liquid, in 55 ~ 70 DEG C, by matter
Amount takes premixed liquid, adjuvant, pretreatment rice washing water, sizing material to be mixed than 6:0.1 ~ 0.3:1 ~ 3:0.1, with 45 ~ 55kHz ultrasonic wave
15 ~ 25min is handled, obtains and compound lures mosquito ingredient;
(5) according to the mass fraction, take 30 ~ 50 parts of garden soil, 20 ~ 40 parts of clays, 15 ~ 30 parts of activated sludge, 1 ~ 4 part of fragrance, 5 ~
10 parts of allethrins, 8 ~ 15 parts of ES Esbiothrins, 6 ~ 10 parts of poly- lysines, 20 ~ 40 parts it is compound lure mosquito ingredient, 10 ~ 20 parts it is standby
Excrement corrosive liquid, 15 ~ 25 parts of spare modified alta-muds, 15 ~ 30 portions of spare concentrates, 20 ~ 40 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, the 20 ~ 30% of premix soil property amount is taken to rely with poly-
Propylhomoserin, it is compound lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, the mixing of spare concentrate, obtain upper layer
Soil takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin,
Up to mosquito eradication potting soil.
Stalk: wheat stalk.
Bacteria agent: according to the mass fraction, 2 parts of thermophilic lactobacillis, 4 parts of lactobacillus plantarums, 2 parts of photosynthetic bacterias, 8 parts of Pasteur are taken
Bacillus, 5 parts of molasses, 15 parts of yeast extracts, 1 part of sodium chloride, 0.2 part of sodium nitrite, 0.4 part of calcium chloride, 800 parts of sterile waters are mixed
It closes, with 200r/min shaking table culture, is sealed by fermentation 3 days to get bacteria agent.
Solvent A: it is 10% silver nitrate solution, sodium secondary alkyl sulfonate mixing to get solvent that 12:1 in mass ratio, which takes mass fraction,
A。
Solvent B: 12:1 in mass ratio takes the PBS buffer solution that pH value is 6.7, NaCl to mix to get solvent B.
5:1 in mass ratio takes wintersweet flower essential oil and Lavender to mix to get essential oil.
Pretreatment rice washing water: take fresh rice washing water in closing transparent vessel in, solarization 3 days, discharge, filtering, take filtrate in
55 DEG C, it is concentrated in vacuo to the 35% of original volume, obtains concentrate, 5:1 in mass ratio takes concentrate and ammonium carbonate to mix to get pre- place
Manage rice washing water.
Adjuvant: 1:1:1:12 in mass ratio takes cellulose, sodium alkyl sulfate, Cocoalkyl oxalamide, glycerol mixing,
Up to adjuvant.
Auxiliary agent: 5:1 in mass ratio takes potassium humate, sodium citrate mixing to get auxiliary agent.Sizing material: lac.
A kind of preparation method of mosquito eradication potting soil, includes the following steps:
(1) according to parts by weight, successively take 30 parts of stalks, 20 parts of wormcasts, 15 parts of duck excrements, 20 parts of urea, 20 parts of bacteria agents,
50 parts of water are mixed in container, after stirring 20min with 300r/min revolving speed, are stacked 7 days in being protected from light ventilation, stack retting material are obtained, by matter
Amount ratio is that 1:6 takes stack retting material and water to mix, and in 95 DEG C of heating boiling 30min, filters while hot, obtains excrement corrosive liquid, spare;
(2) 6:1 in mass ratio takes sodium bentonite, auxiliary agent to crushed 200 meshes in pulverizer, take sieving particle be by volume
The HCl solution of concentration 0.01mol/L is added in 1:3:5, the sodium citrate solution of mass fraction 25% is mixed in reaction kettle, in 45 DEG C,
Under the conditions of 400r/min, constant temperature is stirred to react 2h, and filtering obtains filter cake, takes during filter cake is washed with deionized to cleaning solution and is in
Property after, be transferred to 70 DEG C of oven dryings to constant weight, obtain acidleach bentonite, in 55 DEG C, in mass ratio for 1:30 take acidleach bentonite with
Solvent A mixing, under the conditions of 300r/min, constant temperature is stirred to react 45min, and filtering obtains filter residue, filter residue is taken to be washed with deionized
After filter residue 3 times, 100 DEG C of oven dryings are moved to constant weight, obtain modified alta-mud, it is spare;
(3) glandula digestive for taking fresh long octopus is put into refiner and is homogenized, obtain slurry, take slurry by matter after being rinsed 2 times with water
It measures and the PBS buffer solution mixing that pH value is 6.7 is added than 1:10, with 400r/min magnetic agitation 25min, obtain mixed liquor, take mixing
Ammonium sulfate is added in liquid 7:1 in mass ratio, saltouts, and after 4 DEG C of dialysed overnights, is centrifuged 10min in 1000r/min, takes supernatant
The ammonium sulfate of liquid addition supernatant quality 30% is secondary to saltout, and after 4 DEG C of dialysis, is centrifuged 10min in 1000r/min, takes centrifugation
Object obtains active matter in 1 DEG C of refrigerator cold-storage 20h, takes active matter 1:4:15 in mass ratio that wintersweet flower essential oil is added, pH is 6.5
The mixing of Tris-HCl buffer obtains concentrate in 40 DEG C of vacuum evaporation to the 30% of original volume, spare;
(4) 1:10:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 75 DEG C, keeps the temperature 1h, naturally cold
But, saponification grease is obtained, in 35 DEG C, 5:1:1:20 in mass ratio takes saponification grease, sodium metasilicate, neopelex, water
Mixing, with 400r/min magnetic agitation 20min, obtains premixed liquid, and in 55 DEG C, 6:0.1:1:0.1 in mass ratio takes premixed liquid, auxiliary
Agent, pretreatment rice washing water, sizing material are mixed, and with 45kHz ultrasonication 15min, obtain and compound lure mosquito ingredient;
(5) according to the mass fraction, 30 parts of garden soil, 20 parts of clays, 15 parts of activated sludge, 1 part of fragrance, 5 parts of allethrins, 8 are taken
Part ES Esbiothrin, 6 parts of poly- lysines, 20 parts it is compound lure mosquito ingredient, 10 parts of spare excrement corrosive liquids, 15 parts it is spare
Modified alta-mud, 15 portions of spare concentrates, 20 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, takes 20% and poly- bad ammonia of premix soil property amount
It is sour, compound to lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, spare concentrate mixing, topsoil is obtained,
It takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin, i.e.,
Obtain mosquito eradication potting soil.
Stalk: cotton stalk.
Bacteria agent: according to the mass fraction, 5 parts of thermophilic lactobacillis, 8 parts of lactobacillus plantarums, 5 parts of photosynthetic bacterias, 15 parts bars are taken
Family name bacillus, 10 parts of molasses, 25 parts of yeast extracts, 4 parts of sodium chloride, 0.6 part of sodium nitrite, 0.8 part of calcium chloride, 1000 parts it is sterile
Water mixing is sealed by fermentation 6 days with 250r/min shaking table culture to get bacteria agent.
Solvent A: it is 10% silver nitrate solution, sodium secondary alkyl sulfonate mixing to get solvent that 16:1 in mass ratio, which takes mass fraction,
A。
Solvent B: 18:1 in mass ratio takes the PBS buffer solution that pH value is 7.2, NaCl to mix to get solvent B.
5:2 in mass ratio takes wintersweet flower essential oil and Lavender to mix to get essential oil.
Pretreatment rice washing water: take fresh rice washing water in closing transparent vessel in, solarization 5 days, discharge, filtering, take filtrate in
70 DEG C, it is concentrated in vacuo to the 50% of original volume, obtains concentrate, 8:1 in mass ratio takes concentrate and ammonium carbonate to mix to get pre- place
Manage rice washing water.
Adjuvant: 1:3:1:18 in mass ratio takes cellulose, sodium alkyl sulfate, Cocoalkyl oxalamide, glycerol mixing,
Up to adjuvant.
Auxiliary agent: 5:2 in mass ratio takes potassium humate, sodium citrate mixing to get auxiliary agent.Sizing material: peach gum.
A kind of preparation method of mosquito eradication potting soil, includes the following steps:
(1) according to parts by weight, successively take 50 parts of stalks, 30 parts of wormcasts, 30 parts of duck excrements, 40 parts of urea, 35 parts of bacteria agents,
75 parts of water are mixed in container, after stirring 30min with 400r/min revolving speed, are stacked 10 days in being protected from light ventilation, stack retting material are obtained, by matter
Amount ratio is that 1:10 takes stack retting material and water to mix, and in 100 DEG C of heating boiling 45min, filters while hot, obtains excrement corrosive liquid, spare;
(2) 10:1 in mass ratio takes sodium bentonite, auxiliary agent to crushed 200 meshes in pulverizer, takes sieving particle by volume
It is mixed for the HCl solution of 1:6:5 addition concentration 0.01mol/L, the sodium citrate solution of mass fraction 25% in reaction kettle, in 55
DEG C, with 600r/min under the conditions of, constant temperature is stirred to react 4h, and filtering obtains filter cake, takes filter cake to be washed with deionized to cleaning solution to be in
After neutrality, 90 DEG C of oven dryings are transferred to constant weight, acidleach bentonite is obtained, in 60 DEG C, takes acidleach bentonite in mass ratio for 1:50
It is mixed with solvent A, under the conditions of 500r/min, constant temperature is stirred to react 60min, and filtering obtains filter residue, filter residue is taken to be washed with deionized water
After washing filter residue 5 times, 110 DEG C of oven dryings are moved to constant weight, obtain modified alta-mud, it is spare;
(3) glandula digestive for taking fresh long octopus is put into refiner and is homogenized, obtain slurry, take slurry by matter after being rinsed 5 times with water
It measures and the PBS buffer solution mixing that pH value is 7.2 is added than 1:15, with 600r/min magnetic agitation 50min, obtain mixed liquor, take mixing
Ammonium sulfate is added in liquid 12:1 in mass ratio, saltouts, and after 8 DEG C of dialysed overnights, is centrifuged 15min in 2000r/min, takes supernatant
The ammonium sulfate of liquid addition supernatant quality 50% is secondary to saltout, and after 8 DEG C of dialysis, is centrifuged 15min in 2000r/min, takes centrifugation
Object for 24 hours, obtains active matter in 4 DEG C of refrigerator cold-storages, takes active matter 1:7:15 in mass ratio that wintersweet flower essential oil is added, pH is 6.9
The mixing of Tris-HCl buffer obtains concentrate in 55 DEG C of vacuum evaporation to the 45% of original volume, spare;
(4) 1:15:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 90 DEG C, keeps the temperature 3h, naturally cold
But, saponification grease is obtained, in 50 DEG C, 5:1:2:30 in mass ratio takes saponification grease, sodium metasilicate, neopelex, water
Mixing, with 600r/min magnetic agitation 45min, obtains premixed liquid, and in 70 DEG C, 6:0.3:3:0.1 in mass ratio takes premixed liquid, auxiliary
Agent, pretreatment rice washing water, sizing material are mixed, and with 55kHz ultrasonication 25min, obtain and compound lure mosquito ingredient;
(5) according to the mass fraction, take 50 parts of garden soil, 40 parts of clays, 30 parts of activated sludge, 4 parts of fragrance, 10 parts of allethrins,
15 parts of ES Esbiothrins, 10 parts of poly- lysines, 40 parts it is compound lure mosquito ingredient, 20 parts of spare excrement corrosive liquids, 25 parts it is spare
Modified alta-mud, 30 portions of spare concentrates, 40 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, takes 30% and poly- bad ammonia of premix soil property amount
It is sour, compound to lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, spare concentrate mixing, topsoil is obtained,
It takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin, i.e.,
Obtain mosquito eradication potting soil.
Stalk: rice straw.
Bacteria agent: according to the mass fraction, 3 parts of thermophilic lactobacillis, 5 parts of lactobacillus plantarums, 3 parts of photosynthetic bacterias, 11 parts bars are taken
Family name bacillus, 7 parts of molasses, 17 parts of yeast extracts, 3 parts of sodium chloride, 0.4 part of sodium nitrite, 0.6 part of calcium chloride, 900 parts of sterile waters
Mixing is sealed by fermentation 4 days with 230r/min shaking table culture to get bacteria agent.
Solvent A: it is 10% silver nitrate solution, sodium secondary alkyl sulfonate mixing to get solvent that 14:1 in mass ratio, which takes mass fraction,
A。
Solvent B: 15:1 in mass ratio takes the PBS buffer solution that pH value is 6.9, NaCl to mix to get solvent B.
5:1.5 in mass ratio takes wintersweet flower essential oil and Lavender to mix to get essential oil.
Pretreatment rice washing water: take fresh rice washing water in closing transparent vessel in, solarization 4 days, discharge, filtering, take filtrate in
65 DEG C, it is concentrated in vacuo to the 45% of original volume, obtains concentrate, 7:1 in mass ratio takes concentrate and ammonium carbonate to mix to get pre- place
Manage rice washing water.
Adjuvant: 1:2:1:15 in mass ratio takes cellulose, sodium alkyl sulfate, Cocoalkyl oxalamide, glycerol mixing,
Up to adjuvant.
Auxiliary agent: 5:1.5 in mass ratio takes potassium humate, sodium citrate mixing to get auxiliary agent.Sizing material: xanthan gum.
A kind of preparation method of mosquito eradication potting soil, includes the following steps:
(1) according to parts by weight, successively take 40 parts of stalks, 25 parts of wormcasts, 20 parts of duck excrements, 30 parts of urea, 25 parts of bacteria agents,
60 parts of water are mixed in container, after stirring 25min with 350r/min revolving speed, are stacked 9 days in being protected from light ventilation, stack retting material are obtained, by matter
Amount ratio is that 1:8 takes stack retting material and water to mix, and in 105 DEG C of heating boiling 35min, filters while hot, obtains excrement corrosive liquid, spare;
(2) 8:1 in mass ratio takes sodium bentonite, auxiliary agent to crushed 200 meshes in pulverizer, take sieving particle be by volume
The HCl solution of concentration 0.01mol/L is added in 1:5:5, the sodium citrate solution of mass fraction 25% is mixed in reaction kettle, in 50 DEG C,
Under the conditions of 500r/min, constant temperature is stirred to react 3h, and filtering obtains filter cake, takes during filter cake is washed with deionized to cleaning solution and is in
Property after, be transferred to 80 DEG C of oven dryings to constant weight, obtain acidleach bentonite, in 57 DEG C, in mass ratio for 1:40 take acidleach bentonite with
Solvent A mixing, under the conditions of 400r/min, constant temperature is stirred to react 50min, and filtering obtains filter residue, filter residue is taken to be washed with deionized
After filter residue 4 times, 105 DEG C of oven dryings are moved to constant weight, obtain modified alta-mud, it is spare;
(3) glandula digestive for taking fresh long octopus is put into refiner and is homogenized, obtain slurry, take slurry by matter after being rinsed 3 times with water
It measures and the PBS buffer solution mixing that pH value is 6.9 is added than 1:12, with 500r/min magnetic agitation 35min, obtain mixed liquor, take mixing
Ammonium sulfate is added in liquid 9:1 in mass ratio, saltouts, and after 6 DEG C of dialysed overnights, is centrifuged 13min in 1500r/min, takes supernatant
The ammonium sulfate of liquid addition supernatant quality 40% is secondary to saltout, and after 6 DEG C of dialysis, is centrifuged 13min in 1500r/min, takes centrifugation
Object obtains active matter in 3 DEG C of refrigerator cold-storage 22h, takes active matter 1:5:15 in mass ratio that wintersweet flower essential oil is added, pH is 6.7
The mixing of Tris-HCl buffer obtains concentrate in 45 DEG C of vacuum evaporation to the 35% of original volume, spare;
(4) 1:12:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 80 DEG C, keeps the temperature 2h, naturally cold
But, obtain saponification grease, in 40 DEG C, 5:1:1.5:25 in mass ratio take saponification grease, sodium metasilicate, neopelex,
Water mixing, with 500r/min magnetic agitation 35min, obtains premixed liquid, and in 65 DEG C, 6:0.2:2:0.1 in mass ratio takes premixed liquid, auxiliary
Agent, pretreatment rice washing water, sizing material are mixed, and with 50kHz ultrasonication 19min, obtain and compound lure mosquito ingredient;
(5) according to the mass fraction, 40 parts of garden soil, 30 parts of clays, 25 parts of activated sludge, 3 parts of fragrance, 7 parts of allethrins, 9 are taken
Part ES Esbiothrin, 8 parts of poly- lysines, 30 parts it is compound lure mosquito ingredient, 15 parts of spare excrement corrosive liquids, 20 parts it is spare
Modified alta-mud, 25 portions of spare concentrates, 25 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, takes 25% and poly- bad ammonia of premix soil property amount
It is sour, compound to lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, spare concentrate mixing, topsoil is obtained,
It takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin, i.e.,
Obtain mosquito eradication potting soil.
Comparative example: the mosquito killing liquid of company of Chongqing City production.
The mosquito killing liquid of the mosquito eradication potting soil of embodiment preparation and comparative example is subjected to mosquito eradication experiment test,
Test object: Culex quinquefasciatus 200;Closed transparent vessel: 40cm × 30cm × 30cm;Since Culex quinquefasciatus happiness night inhales
Blood mosquito kind, laboratory darkness is protected from light during test, and covers closed container with black cloth.The testing crew right hand smears plant mosquito repellent
Liquid protrudes into the closed container for filling 100 Culex quinquefasciatus, while being put into the closed container for filling 100 Culex quinquefasciatus
Mosquito eradication potting soil is as control.Test situation is observed in 1h, 2h, 3h, 4h respectively, checks flight mosquito number in closed container.
Effect exterminating mosquito=(flight mosquito number in 100- closed container) × 100%, effect exterminating mosquito test data is shown in Table 1.
Table 1:
Effect exterminating mosquito | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
1h | 100% | 100% | 100% | 95% |
2h | 97% | 96% | 98% | 82% |
3h | 89% | 88% | 89% | 70% |
4h | 80% | 83% | 85% | 53% |
In conclusion as shown in Table 1, mosquito eradication potting soil effect exterminating mosquito prepared by the present invention is good, it is worth advocating using energetically.
Claims (10)
1. a kind of preparation method of mosquito eradication potting soil, which is characterized in that the preparation method includes the following steps:
(1) according to parts by weight, successively take 30 ~ 50 parts of stalks, 20 ~ 30 parts of wormcasts, 15 ~ 30 parts of duck excrements, 20 ~ 40 parts of urea,
20 ~ 35 parts of bacteria agents, 50 ~ 75 parts of water are mixed, and are protected from light ventilation and stack, obtain stack retting material, take heap in mass ratio for 1:6 ~ 10
Material and water mixing are macerated, boiling is heated, filters while hot, obtain excrement corrosive liquid, it is spare;
(2) 6 ~ 10:1 in mass ratio takes sodium bentonite, auxiliary agent to pulverize and sieve, and take sieving particle is that 1:3 ~ 6:5 adds by volume
Entering HCl solution, sodium citrate solution mixing, constant temperature is stirred to react, filters, obtain filter cake, filter cake is taken to be washed with water, and it is dry, it obtains sour
It soaks bentonite and takes acidleach bentonite to mix with solvent A for 1:30 ~ 50 in mass ratio in 55 ~ 60 DEG C, constant temperature stirring, filtering obtains
Filter residue takes filter residue to wash, dry, obtains modified alta-mud, spare;
(3) glandula digestive of fresh long octopus is taken, is rinsed, homogenate obtains slurry, takes slurry 1:10 in mass ratio ~ 15 that solvent B mixing is added
Stirring, obtains mixed liquor, takes mixed liquor 7 ~ 12:1 in mass ratio that ammonium sulfate is added, saltouts, and dialyses, centrifugation, and supernatant is taken to be added
The ammonium sulfate of clear liquid quality 30 ~ 50%, saltouts, and dialyses, centrifugation, takes centrifugation object refrigeration, obtains active matter, take active matter in mass ratio
Essential oil, the mixing of Tris-HCl buffer is added in 1:4 ~ 7:15, is evaporated in vacuo, obtains concentrate, spare;
(4) 1:10 ~ 15:3 in mass ratio takes caustic soda, water, pigskin grease to be mixed evenly, and is warming up to 75 ~ 90 DEG C, heat preservation is cold
But, saponification grease is obtained, in 35 ~ 50 DEG C, 5:1:1 ~ 2:20 ~ 30 take saponification grease, sodium metasilicate, detergent alkylate sulphur in mass ratio
Sour sodium, water are mixed, and obtain premixed liquid, and in 55 ~ 70 DEG C, 6:0.1 ~ 0.3:1 in mass ratio ~ 3:0.1 takes premixed liquid, adjuvant, pre-
Handle rice washing water, sizing material is mixed, ultrasonication obtains and compound lures mosquito ingredient;
(5) according to the mass fraction, take 30 ~ 50 parts of garden soil, 20 ~ 40 parts of clays, 15 ~ 30 parts of activated sludge, 1 ~ 4 part of fragrance, 5 ~
10 parts of allethrins, 8 ~ 15 parts of ES Esbiothrins, 6 ~ 10 parts of poly- lysines, 20 ~ 40 parts it is compound lure mosquito ingredient, 10 ~ 20 parts it is standby
Excrement corrosive liquid, 15 ~ 25 parts of spare modified alta-muds, 15 ~ 30 portions of spare concentrates, 20 ~ 40 parts of water;
(6) garden soil, clay, activated sludge mixing are first taken, premix earth material is obtained, the 20 ~ 30% of premix soil property amount is taken to rely with poly-
Propylhomoserin, it is compound lure mosquito ingredient, spare excrement corrosive liquid, spare modified alta-mud, the mixing of spare concentrate, obtain upper layer
Soil takes the topsoil of obtained quality to be placed on remaining premix earth material, is finally uniformly put into fragrance, allethrin, ES Esbiothrin,
Up to mosquito eradication potting soil.
2. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that the straw in the step (1)
Stalk: wheat stalk, cotton stalk, any one in rice straw.
3. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that the life in the step (1)
Object microbial inoculum: according to the mass fraction, 2 ~ 5 parts of thermophilic lactobacillis, 4 ~ 8 parts of lactobacillus plantarums, 2 ~ 5 parts of photosynthetic bacterias, 8 ~ 15 parts of Pasteur are taken
Bacillus, 5 ~ 10 parts of molasses, 15 ~ 25 parts of yeast extracts, 1 ~ 4 part of sodium chloride, 0.2 ~ 0.6 part of sodium nitrite, 0.4 ~ 0.8 part of chlorination
Calcium, 800 ~ 1000 parts of sterile water mixing, shaking table culture are sealed by fermentation to get bacteria agent.
4. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that helping in the step (2)
Agent: 5:1 ~ 2 take potassium humate, sodium citrate mixing to get auxiliary agent in mass ratio.
5. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that molten in the step (2)
Agent A: 12 ~ 16:1 in mass ratio takes silver nitrate solution, sodium secondary alkyl sulfonate mixing to get solvent A.
6. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that molten in the step (3)
Agent B: 12 ~ 18:1 in mass ratio takes PBS buffer solution, NaCl mixing to get solvent B.
7. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that the essence in the step (3)
Oil: 5:1 ~ 2 take wintersweet flower essential oil and Lavender to mix to get essential oil in mass ratio.
8. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that auxiliary in the step (4)
Agent: in mass ratio 1:1 ~ 3:1:12 ~ 18 take cellulose, sodium alkyl sulfate, Cocoalkyl oxalamide, glycerol mixing to get
Adjuvant.
9. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that pre- in the step (4)
Processing rice washing water: taking fresh rice washing water sealed light-transmitting solarization, discharges, and filtering takes filter vacuum to be concentrated, obtains concentrate, by quality
Concentrate and ammonium carbonate is taken to mix to get pretreatment rice washing water than 5 ~ 8:1.
10. a kind of preparation method of mosquito eradication potting soil according to claim 1, which is characterized in that in the step (4)
Sizing material: lac, peach gum, any one in xanthan gum.
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CN115152643A (en) * | 2022-08-12 | 2022-10-11 | 杭州高爷家小炮宠物用品有限责任公司 | Preparation process of cereal puffed cat litter |
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CN106035425A (en) * | 2016-06-12 | 2016-10-26 | 查日开 | Mosquito-repellent incense liquid with plant essential oil and method for preparing mosquito-repellent incense liquid |
CN106973941A (en) * | 2017-05-03 | 2017-07-25 | 明光市飞洲新材料有限公司 | A kind of attapulgite modified efficient mosquito lures agent and preparation method thereof of going out |
CN108358727A (en) * | 2018-03-21 | 2018-08-03 | 合肥雪立歆农业科技有限公司 | A kind of soil conditioner and preparation method thereof for flower planting |
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2018
- 2018-08-09 CN CN201810900204.9A patent/CN109053239A/en not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106035425A (en) * | 2016-06-12 | 2016-10-26 | 查日开 | Mosquito-repellent incense liquid with plant essential oil and method for preparing mosquito-repellent incense liquid |
CN106973941A (en) * | 2017-05-03 | 2017-07-25 | 明光市飞洲新材料有限公司 | A kind of attapulgite modified efficient mosquito lures agent and preparation method thereof of going out |
CN108358727A (en) * | 2018-03-21 | 2018-08-03 | 合肥雪立歆农业科技有限公司 | A kind of soil conditioner and preparation method thereof for flower planting |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115152643A (en) * | 2022-08-12 | 2022-10-11 | 杭州高爷家小炮宠物用品有限责任公司 | Preparation process of cereal puffed cat litter |
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Application publication date: 20181221 |