CN109044948A - 一种自体胶原蛋白水光针及其制备方法 - Google Patents
一种自体胶原蛋白水光针及其制备方法 Download PDFInfo
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- CN109044948A CN109044948A CN201811163499.2A CN201811163499A CN109044948A CN 109044948 A CN109044948 A CN 109044948A CN 201811163499 A CN201811163499 A CN 201811163499A CN 109044948 A CN109044948 A CN 109044948A
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Abstract
本发明实施例公开了一种自体胶原蛋白水光针及其制备方法，所述自体胶原蛋白水光针包括以下重量份数的组分：自体胶原蛋白10‑20份、玻尿酸10‑20份、生物多糖胶5‑10份、羊胎素提取液5‑10份、左旋C原液3‑8份、葡萄籽提取物2‑3份、果聚糖2‑5份、β‑葡聚糖2‑5份、维生素C 1‑5份、氨基酸2‑5份、辅酶2‑3份、抗敏剂2‑4份、抗氧化剂2‑5份、防晒剂1‑3份、保湿剂3‑8份，该水光针由人体静脉血提取的自体胶原蛋白制成，源自自体，不会产生排异性，含有细胞修复和生长所需的多种营养成分，在加速皮肤和粘膜创伤愈合、促进细胞生长、促进伤口愈合和增强免疫系统方面有较强的的功效。
Description
技术领域
本发明涉及水光针美容美肤技术领域，具体涉及一种自体胶原蛋白水光针及其制备方法。
背景技术
水光针美容是一种注射类的护肤疗法，注射时要借助专门的仪器“水光枪”，将需要的美容针剂注射到紧贴表皮下的真皮层，注射后能让皮肤变得水润，光亮而得名，水光针主要是以注射玻尿酸为主，玻尿酸又称为透明质酸，是人体组织中自然存在的物质，它可以吸取自身重量1000倍的水分，具有惊人的吸水、锁水和水分循环的作用，除了玻尿酸之外，通常还会注入胶原蛋白，胶原蛋白可以起到填充和支撑弹性纤维的作用，保持皮肤的弹性和光滑，但是由于自身免疫系统对于外部介质的排异性，很可能会出现红、肿、疼痛等副作用，如果体内营养不足还可能导致术后修复紊乱，导致皮肤塌陷、不规律增生和部分细胞严重缺乏营养使得皮层坏死产生凹凸不平，另外由于皮下注射引起的创口如果没有得到较好的护理也会发生感染、发炎等症状。
本发明实施例提出了一种自体胶原蛋白水光针，由人体静脉血提取的富集大量血小板和胶原蛋白的自体胶原蛋白制成，可刺激胶原蛋白、弹性纤维、胶质等的产生，促使组织再生，源自自体，不会产生排异性，无毒无副作用，同时含有细胞修复和生长所需的多种营养成分，能促进细胞的增殖分化，帮助角质细胞分化、存活、延缓衰老，还辅以抗敏剂、抗氧化剂份、防晒剂、保湿剂等成分，能消炎镇痛，防晒保湿，有效防止创口感染，在加速皮肤和粘膜创伤愈合、促进细胞生长、促进伤口愈合和增强免疫系统方面有较强的的功效。
发明内容
本发明实施例的目的在于提供一种自体胶原蛋白水光针及其制备方法，用以解决现有的水光针注射药剂存在排异性，会出现红、肿、疼痛等副作用，营养不足导致术后修复紊乱、皮肤凹陷不平，注射创口易发生感染等问题。
为实现上述目的，本发明实施例提供一种自体胶原蛋白水光针：所述自体胶原蛋白水光针包括以下重量份数的组分：自体胶原蛋白10-20份、玻尿酸10-20份、生物多糖胶5-10份、羊胎素提取液5-10份、左旋C原液3-8份、葡萄籽提取物2-3份、果聚糖2-5份、β-葡聚糖2-5份、维生素C 1-5份、氨基酸2-5份、辅酶2-3份、抗敏剂2-4份、抗氧化剂2-5份、防晒剂1-3份、保湿剂3-8份。
优选的，所述氨基酸选自甘氨酸、丙氨酸、脯氨酸、羟赖氨酸和羟脯氨酸中的一种或几种。
优选的，所述辅酶选自辅酶Q10、烟酰胺和维生素B2中的一种或几种。
优选的，所述抗敏剂选自奥亭敏、甘草酸二钾和粉防己提取物中的一种或几种。
优选的，所述抗氧化剂选自花青素、超氧化物歧化酶、水溶性维生素E和茶多酚中的一种或几种。
优选的，所述防晒剂选自甘光草定、芸香叶苷和虎杖苷中的一种或几种。
优选的，所述保湿剂选自丙三醇、海藻糖和泛醇中的一种或几种。
本发明实施例还提出了一种自体胶原蛋白水光针的制备方法，所述制备方法包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按比例混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)按照各组分相应的重量份数称取各组分，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至290-380mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
优选的，所述步骤1)中，血浆层和白膜层液体按照质量比2:1混合。
本发明实施例具有如下优点：
本发明实施例提出了一种自体胶原蛋白水光针，由人体静脉血提取的富集大量血小板和胶原蛋白的自体胶原蛋白制成，血浆层主要包含血浆、水、蛋白质、盐类和各种离子等，白膜层主要包括富含血小板区、富含淋巴细胞区、富含单核细胞区和富含粒细胞区，提取的血浆层和白膜层混合液中含有大量胶原蛋白，同时富含血小板和各种生长因子，进入真皮浅层后，可刺激胶原蛋白、弹性纤维、胶质等的产生，促使组织再生，源自自体，不会产生排异性，无毒无副作用，生物多糖胶与玻尿酸互补协同，能实现长效锁水保湿，所加入的羊胎素提取液、左旋C原液、葡萄籽提取物、果聚糖、β-葡聚糖、维生素C、氨基酸2、辅酶，能提供细胞修复和生长所需的多种营养成分，促进细胞的增殖分化，帮助角质细胞分化、存活、延缓衰老，另外还加入有抗敏剂、抗氧化剂份、防晒剂、保湿剂，能消炎镇痛，防晒保湿，有效防止创口感染，在加速皮肤和粘膜创伤愈合，促进细胞生长、促进伤口愈合和增强免疫系统方面有较强的的功效。
具体实施方式
以下实施例用于说明本发明，但不用来限制本发明的范围。
实施例1
本实施例的一种自体胶原蛋白水光针的制备方法，包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按质量比2:1混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)称取自体胶原蛋白10份、玻尿酸10份、生物多糖胶5份、羊胎素提取液5份、左旋C原液3份、葡萄籽提取物2份、果聚糖2份、β-葡聚糖2份、维生素C 1份、甘氨酸1份、丙氨酸1份、辅酶Q102份、奥亭敏2份、茶多酚2份、甘光草定1份、丙三醇1份、泛醇2份，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至290-380mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得将各组分溶于注射用水中混合，调节溶液的PH值至7.0±0.5，渗透压调至350mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
实施例2
本实施例的一种自体胶原蛋白水光针的制备方法，包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按质量比2:1混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)称取自体胶原蛋白20份、玻尿酸20份、生物多糖胶10份、羊胎素提取液10份、左旋C原液8份、葡萄籽提取物3份、果聚糖5份、β-葡聚糖5份、维生素C 5份、甘氨酸1份、丙氨酸1份、脯氨酸1份、羟赖氨酸1份、羟脯氨酸1份、辅酶Q102份、烟酰胺1份、奥亭敏2份、甘草酸二钾1份、粉防己提取物1份、花青素3份、茶多酚2份、芸香叶苷2份、虎杖苷1份、丙三醇3份、海藻糖3份、泛醇2份，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至330mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
实施例3
本实施例的一种自体胶原蛋白水光针的制备方法，包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按质量比2:1混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)称取自体胶原蛋白15份、玻尿酸15份、生物多糖胶8份、羊胎素提取液8份、左旋C原液6份、葡萄籽提取物3份、果聚糖3份、β-葡聚糖3份、维生素C 3份、甘氨酸1份、丙氨酸1份、脯氨酸1份、羟赖氨酸1份、羟脯氨酸1份、辅酶Q102份、维生素B21份、奥亭敏2份、甘草酸二钾1份、超氧化物歧化酶2份、茶多酚1份、甘光草定2份、海藻糖3份、泛醇3份，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至300mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
实施例4
本实施例的一种自体胶原蛋白水光针的制备方法，包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按质量比2:1混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)称取自体胶原蛋白15份、玻尿酸20份、生物多糖胶8份、羊胎素提取液7份、左旋C原液5份、葡萄籽提取物3份、果聚糖3份、β-葡聚糖3份、维生素C 4份、甘氨酸1份、丙氨酸1份、脯氨酸1份、羟赖氨酸1份、羟脯氨酸1份、辅酶Q102份、奥亭敏2份、花青素3份、芸香叶苷1份、虎杖苷1份、泛醇3份，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至340mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
实施例5
本实施例的一种自体胶原蛋白水光针的制备方法，包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按质量比2:1混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)称取自体胶原蛋白20份、玻尿酸20份、生物多糖胶10份、羊胎素提取液8份、左旋C原液5份、葡萄籽提取物3份、果聚糖4份、β-葡聚糖4份、维生素C4份、甘氨酸1份、丙氨酸1份、脯氨酸1份、羟赖氨酸1份、羟脯氨酸1份、烟酰胺2份、奥亭敏2份、超氧化物歧化酶1份、水溶性维生素E 1份、甘光草定1份、海藻糖2份、泛醇3份，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至350mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
虽然，上文中已经用一般性说明及具体实施例对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。
Claims (9)
1.一种自体胶原蛋白水光针，其特征在于，所述自体胶原蛋白水光针包括以下重量份数的组分：自体胶原蛋白10-20份、玻尿酸10-20份、生物多糖胶5-10份、羊胎素提取液5-10份、左旋C原液3-8份、葡萄籽提取物2-3份、果聚糖2-5份、β-葡聚糖2-5份、维生素C 1-5份、氨基酸2-5份、辅酶2-3份、抗敏剂2-4份、抗氧化剂2-5份、防晒剂1-3份、保湿剂3-8份。
2.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述氨基酸选自甘氨酸、丙氨酸、脯氨酸、羟赖氨酸和羟脯氨酸中的一种或几种。
3.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述辅酶选自辅酶Q10、烟酰胺和维生素B2中的一种或几种。
4.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述抗敏剂选自奥亭敏、甘草酸二钾和粉防己提取物中的一种或几种。
5.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述抗氧化剂选自花青素、超氧化物歧化酶、水溶性维生素E和茶多酚中的一种或几种。
6.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述防晒剂选自甘光草定、芸香叶苷和虎杖苷中的一种或几种。
7.根据权利要求1所述的一种自体胶原蛋白水光针，其特征在于，所述保湿剂选自丙三醇、海藻糖和泛醇中的一种或几种。
8.根据权利要求1-7中任一所述的一种自体胶原蛋白水光针的制备方法，其特征在于，所述制备方法包括以下步骤：
1)自体胶原蛋白的制备：通过采血针对人体进行采血，血液经首次离心后进入全血分离器，并在全血分离器内进行二次离心，全血离心分为三层，自上而下依次为血浆层、白膜层和红细胞层，将血浆层、白膜层和红细胞层的液体分别通过导管导入不同的储存单元分开存放，由储存单元取出适量血浆层和白膜层液体按比例混合，并经层析单元进行初步纯化、透滤单元进一步纯化即得所需的自体胶原蛋白，置于4℃下冷藏。
2)按照各组分相应的重量份数称取各组分，将除了自体胶原蛋白之外的其他组分溶于注射用水中混合，再将称取好的自体胶原蛋白与其他组分的混合液混合，调节溶液的PH值至7.0±0.5，渗透压调至290-380mOsm/kg·H2O，再经微滤膜除菌过滤、分装、再灭菌既得。
9.根据权利要求8所述的一种自体胶原蛋白水光针的制备方法，其特征在于，所述步骤1)中，血浆层和白膜层液体按照质量比2:1混合。
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Application publication date: 20181221 |