CN109042665A - A kind of method and application for promoting tomato silicon to absorb with epiphysin - Google Patents
A kind of method and application for promoting tomato silicon to absorb with epiphysin Download PDFInfo
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- CN109042665A CN109042665A CN201810603913.0A CN201810603913A CN109042665A CN 109042665 A CN109042665 A CN 109042665A CN 201810603913 A CN201810603913 A CN 201810603913A CN 109042665 A CN109042665 A CN 109042665A
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- tomato
- epiphysin
- silicon
- reagent
- absorb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method and its application for promoting tomato silicon to absorb with epiphysin, comprising the following steps: (1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;(2) during 08:00-10:00, configured epiphysin reagent is sprayed on the tomato seedling blade face of scarce silicon strip part or is added in the scarce silicon nutrient solution of Growth of Tomato Seedling, absorption of the tomato to element silicon in nutrient solution can be improved by adding external source epiphysin in the present invention, the Net Photosynthetic Rate of tomato is improved simultaneously, increase the content of blade Determination of Chlorophyll, due to the promotion of tomato element silicon absorbability, silicone content increases in plant pericarp and pulp, so as to effectively prevent the diseases such as blossom-end rot to occur, improve the disease resistance ability of tomato, be conducive to the growth and development of tomato.
Description
Technical field
The present invention relates to crop planting technical field, it is specially a kind of with epiphysin promote tomato silicon absorb method and
It is applied.
Background technique
Tomato is one of the main vegetables kind cultivated extensively in the world, and tomato plant life can be remarkably promoted by rationally applying silicon
It is long, it blooms ahead of time, increases the content of tomato yield, fruit Vc, beta carotene and lycopene, in addition, silicon is to tomato growth
And leaf photosynthesis and transpiration tool have a certain impact.Silicon is the second element abundant in the earth's crust and soil, but mostly with
Silicate crystalline or precipitation form exist, and the content for the effective silicon that can be absorbed and used by plants is lower, and root system of plant is with active
Mode absorbs SiO3 2-, after a series of chemical reaction, generate dioxide/silica gel (SiO2·nH2O) and it is distributed in plant
On epidermal cell and cell wall.
Epiphysin (n-acetyl-5-methoxytryptamine, Melatonin, MT) is mainly the pine by mammal and the mankind
A kind of structure that fruit body generates is simple, low molecular weight indole hormone, is equally widely present in plant, although content is few
(usually with the presence of pg or ng level) but plays very important effect in terms of Physiological effect, increase stress resistance of plant.It takes off black
Element has very strong anti-radical action, can activate the anti-of crop under the Abiotic stress conditions such as high temperature, low temperature, arid, dim light
Oxidase active.MT can also safeguard the integrality of chloroplast structure under Abiotic stress conditions, guarantee the normal fortune of Photosynthetic
Turn.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for promoting tomato silicon to absorb with epiphysin to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the invention provides the following technical scheme: a kind of side for promoting tomato silicon to absorb with epiphysin
Method, comprising the following steps:
(1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;
(2) during 08:00-10:00, configured epiphysin reagent is sprayed on to the tomato seedling blade face of scarce silicon strip part
Or it adds in the scarce silicon nutrient solution of Growth of Tomato Seedling.
Preferably, the added solvent is that ethyl alcohol and methanol mixed configuration form, and mixed proportion is 5:3.
Preferably, the configuration method of the epiphysin reagent is first to dissolve epiphysin with added solvent, finally with distillation
Water carries out constant volume to the mixed liquor of epiphysin and additive reagent.
Preferably, in the step (2) when tomato growth to 2 leaf, 1 heart and Growth of Tomato Seedling are bloomed to the first flower spike
The epiphysin reagent of epiphysin reagent and 150 μm of ol/L that spraying concentration is 100 μm of ol/L, spray are respectively corresponded to tomato seedling
Applying number is 2-3 times, and is sprayed once every 3-5 days.
Preferably, a kind of method for promoting tomato silicon to absorb with epiphysin is on promoting Development of Tomato resistance
Using.
Compared with prior art, the beneficial effects of the present invention are:
(1) by spraying external source epiphysin to tomato plants, the content of tomato leaf Determination of Chlorophyll is improved, tomato is accelerated
Net Photosynthetic Rate promotes tomato assimilative capacicy, shortens the growth cycle of tomato.
(2) absorptivity that element silicon in nutrient solution can be improved increases each organ silicone content of tomato, and tomato is higher
The generation of the diseases such as blossom-end rot can be effectively prevented in silicone content, improves tomato disease resistance ability.
(3) epiphysin reagent is configured by added solvent and can reduce epiphysin to tomato plants with distilled water constant volume
The toxicity of seedling.
(4) inherently a kind of antioxidant of spectrum of epiphysin reagent, no pollution to the environment, and external source supplement take off black
Tomato be can be improved after element to the resistance of greenhouse abiotic stress.
Detailed description of the invention
Fig. 1 is under the conditions of lacking silicon and external source supplements tomato leaf chlorophyll content comparison diagram after epiphysin;
Fig. 2 is under the conditions of lacking silicon and external source supplements tomato leaf Net Photosynthetic Rate comparison diagram after epiphysin;
Fig. 3 is under the conditions of lacking silicon and external source supplements tomato peel silicone content comparison diagram after epiphysin;
Fig. 4 is under the conditions of lacking silicon and external source supplements tomato pulp silicone content comparison diagram after epiphysin.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-4, a kind of embodiment provided by the invention: be technical solution in the embodiment of the present invention below into
Row clearly and completely describes, it is clear that described embodiments are only a part of the embodiments of the present invention, rather than whole realities
Apply example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work
Every other embodiment, shall fall within the protection scope of the present invention.
Embodiment one
The application that epiphysin promotes silicon to absorb in tomato growth
(1) nursery: full, uniform tomato (Solanum lycopersicum L.) ' rouge and powder champion ' kind is chosen
Son is chosen the neat seed that germinates and is sowed at equipped with tomato dedicated substrate (coco bran: mushroom slag: pearl using conventional method presoaking and germinating
Rock=2V:2V:1V) in nursery.It is washed with deionized water root matrix after neat emergence, is trained using Hoagland nutrient solution
It supports.It will wherein 1/2 seedling be put into the nutrient solution of scarce silicon (1/2Hoagland), remainder is put into normal Hoagland nutrition
Liquid compares;
(2) it prepares epiphysin reagent: being the proportional arrangement added solvent of 5:3 first, in accordance with ethyl alcohol and methanol, then will take off black
Element is dissolved with added solvent, finally carries out constant volume with mixed liquor of the distilled water to epiphysin and additive reagent, and prepare out concentration
For the epiphysin reagent of 100 μm of ol/L and 150 μm of ol/L, and prepared epiphysin reagent is placed on to 4 DEG C or so of sealing ring
Border is saved;
(3) spray: when growth of seedling to 21 heart of leaf, morning 08:00-10:00 spraying concentration is taking off for 100 μm of ol/L
Melanocyte reagent was sprayed once every 3 days, is sprayed altogether 2 times, and until growth of seedling is bloomed to the first flower spike, selection is same again
Sample time point spraying concentration is the epiphysin reagent of 150 μm of ol/L, sprayed 1 time, sprays 3 times every 5 days;
(4) leaf chlorophyll and Net Photosynthetic Rate detection: florescence 10:00-11:00 samples blade, takes under growing point the
Five leaves measure tomato leaf chlorophyll content and net photosynthetic rate;
(5) silicone content detects: fruiting period, takes Tomato Root System, stalk, blade, pericarp, pulp separation drying, weighs drying
Sample 0.3g is crushed, porcelain crucible is put into, 300 DEG C of pre- ashing 3h, are warming up to 550 DEG C of ashing 4h in Muffle furnace.Sample will be ashed
With 48ml 0.08mol/L H2SO4It washes in clean polyethylene bottle, adds 2ml 40%HF, shaking table shaken overnight, filtering.It takes
2ml filtrate is transferred in new polyethylene bottle, and 50ml 0.32%H is added3BO3, vibrate 2h.10ml mixed liquor is taken, 5ml is added
H2SO4Ammonium molybdate mixed liquor [H2SO4: ammonium molybdate 1:1 (v/v), H2SO4Concentration 0.08mol/L, (NH4)6Mo7O24·4H2O is dense
Spend 20g/L], oscillating reactions 5min, is then added 3.3% tartaric acid of 5ml and 5ml 0.4% restores type ascorbic acid, and oscillation is anti-
25min is answered, absorbance is measured at 811nm, 3 repetitions calculate average value.
(6) according to above step measure under the conditions of scarce silicon and external source supplement epiphysin after Tomato Root System, stalk, blade silicon contain
Amount comparison diagram is illustrated in fig. 1 shown below:
Note: with significant (P < 0.05) of lowercase letter indication difference different after column data in table
As can be seen from the figure spraying epiphysin to scarce silicon tomato can be improved the content of chlorophyll and the net light of tomato
Rate is closed, promotes the absorption of element silicon during Development of Tomato, due to the promotion of tomato element silicon absorbability, plant fruit
Silicone content increases in skin and pulp, so as to effectively prevent the diseases such as blossom-end rot to occur, improves the disease resistance ability of tomato, has
Conducive to the growth and development of tomato.
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art,
It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc.
With replacement, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this
Within the protection scope of invention.
Claims (5)
1. a kind of method for promoting tomato silicon to absorb with epiphysin, it is characterised in that: the following steps are included:
(1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;
(2) during 08:00-10:00, configured epiphysin reagent is sprayed on to tomato seedling blade face or the throwing of scarce silicon strip part
It is added in the scarce silicon nutrient solution of Growth of Tomato Seedling.
2. according to claim 1 it is a kind of with epiphysin promote tomato silicon absorb method, it is characterised in that: it is described add it is molten
Agent is that ethyl alcohol and methanol mixed configuration form, and mixed proportion is 5:3.
3. a kind of method for promoting tomato silicon to absorb with epiphysin according to claim 1, it is characterised in that: the epiphysin
The configuration method of reagent is first to dissolve epiphysin with added solvent, the finally mixing with distilled water to epiphysin and additive reagent
Liquid carries out constant volume.
4. a kind of method for promoting tomato silicon to absorb with epiphysin according to claim 1, it is characterised in that: the step
(2) tomato seedling is respectively corresponded when tomato growth to 2 leaf, 1 heart and Growth of Tomato Seedling are bloomed to the first flower spike in spray it is dense
Degree is the epiphysin reagent of 100 μm of ol/L and the epiphysin reagent of 150 μm of ol/L, and spraying number was 2-3 times, and every 3-5 days
It sprays primary.
5. a kind of method for promoting tomato silicon to absorb with epiphysin described in claim 1 is on promoting Development of Tomato resistance
Application.
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Cited By (1)
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CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
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2018
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
CN110679364B (en) * | 2019-09-27 | 2022-03-25 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
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