CN109042665A - A kind of method and application for promoting tomato silicon to absorb with epiphysin - Google Patents

A kind of method and application for promoting tomato silicon to absorb with epiphysin Download PDF

Info

Publication number
CN109042665A
CN109042665A CN201810603913.0A CN201810603913A CN109042665A CN 109042665 A CN109042665 A CN 109042665A CN 201810603913 A CN201810603913 A CN 201810603913A CN 109042665 A CN109042665 A CN 109042665A
Authority
CN
China
Prior art keywords
tomato
epiphysin
silicon
reagent
absorb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201810603913.0A
Other languages
Chinese (zh)
Other versions
CN109042665B (en
Inventor
徐宁
张方园
王闯
曹娜
刘敏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liaocheng Vocational and Technical College
Original Assignee
Liaocheng Vocational and Technical College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liaocheng Vocational and Technical College filed Critical Liaocheng Vocational and Technical College
Priority to CN201810603913.0A priority Critical patent/CN109042665B/en
Publication of CN109042665A publication Critical patent/CN109042665A/en
Application granted granted Critical
Publication of CN109042665B publication Critical patent/CN109042665B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of method and its application for promoting tomato silicon to absorb with epiphysin, comprising the following steps: (1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;(2) during 08:00-10:00, configured epiphysin reagent is sprayed on the tomato seedling blade face of scarce silicon strip part or is added in the scarce silicon nutrient solution of Growth of Tomato Seedling, absorption of the tomato to element silicon in nutrient solution can be improved by adding external source epiphysin in the present invention, the Net Photosynthetic Rate of tomato is improved simultaneously, increase the content of blade Determination of Chlorophyll, due to the promotion of tomato element silicon absorbability, silicone content increases in plant pericarp and pulp, so as to effectively prevent the diseases such as blossom-end rot to occur, improve the disease resistance ability of tomato, be conducive to the growth and development of tomato.

Description

A kind of method and application for promoting tomato silicon to absorb with epiphysin
Technical field
The present invention relates to crop planting technical field, it is specially a kind of with epiphysin promote tomato silicon absorb method and It is applied.
Background technique
Tomato is one of the main vegetables kind cultivated extensively in the world, and tomato plant life can be remarkably promoted by rationally applying silicon It is long, it blooms ahead of time, increases the content of tomato yield, fruit Vc, beta carotene and lycopene, in addition, silicon is to tomato growth And leaf photosynthesis and transpiration tool have a certain impact.Silicon is the second element abundant in the earth's crust and soil, but mostly with Silicate crystalline or precipitation form exist, and the content for the effective silicon that can be absorbed and used by plants is lower, and root system of plant is with active Mode absorbs SiO3 2-, after a series of chemical reaction, generate dioxide/silica gel (SiO2·nH2O) and it is distributed in plant On epidermal cell and cell wall.
Epiphysin (n-acetyl-5-methoxytryptamine, Melatonin, MT) is mainly the pine by mammal and the mankind A kind of structure that fruit body generates is simple, low molecular weight indole hormone, is equally widely present in plant, although content is few (usually with the presence of pg or ng level) but plays very important effect in terms of Physiological effect, increase stress resistance of plant.It takes off black Element has very strong anti-radical action, can activate the anti-of crop under the Abiotic stress conditions such as high temperature, low temperature, arid, dim light Oxidase active.MT can also safeguard the integrality of chloroplast structure under Abiotic stress conditions, guarantee the normal fortune of Photosynthetic Turn.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for promoting tomato silicon to absorb with epiphysin to solve above-mentioned background skill The problem of being proposed in art.
To achieve the above object, the invention provides the following technical scheme: a kind of side for promoting tomato silicon to absorb with epiphysin Method, comprising the following steps:
(1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;
(2) during 08:00-10:00, configured epiphysin reagent is sprayed on to the tomato seedling blade face of scarce silicon strip part Or it adds in the scarce silicon nutrient solution of Growth of Tomato Seedling.
Preferably, the added solvent is that ethyl alcohol and methanol mixed configuration form, and mixed proportion is 5:3.
Preferably, the configuration method of the epiphysin reagent is first to dissolve epiphysin with added solvent, finally with distillation Water carries out constant volume to the mixed liquor of epiphysin and additive reagent.
Preferably, in the step (2) when tomato growth to 2 leaf, 1 heart and Growth of Tomato Seedling are bloomed to the first flower spike The epiphysin reagent of epiphysin reagent and 150 μm of ol/L that spraying concentration is 100 μm of ol/L, spray are respectively corresponded to tomato seedling Applying number is 2-3 times, and is sprayed once every 3-5 days.
Preferably, a kind of method for promoting tomato silicon to absorb with epiphysin is on promoting Development of Tomato resistance Using.
Compared with prior art, the beneficial effects of the present invention are:
(1) by spraying external source epiphysin to tomato plants, the content of tomato leaf Determination of Chlorophyll is improved, tomato is accelerated Net Photosynthetic Rate promotes tomato assimilative capacicy, shortens the growth cycle of tomato.
(2) absorptivity that element silicon in nutrient solution can be improved increases each organ silicone content of tomato, and tomato is higher The generation of the diseases such as blossom-end rot can be effectively prevented in silicone content, improves tomato disease resistance ability.
(3) epiphysin reagent is configured by added solvent and can reduce epiphysin to tomato plants with distilled water constant volume The toxicity of seedling.
(4) inherently a kind of antioxidant of spectrum of epiphysin reagent, no pollution to the environment, and external source supplement take off black Tomato be can be improved after element to the resistance of greenhouse abiotic stress.
Detailed description of the invention
Fig. 1 is under the conditions of lacking silicon and external source supplements tomato leaf chlorophyll content comparison diagram after epiphysin;
Fig. 2 is under the conditions of lacking silicon and external source supplements tomato leaf Net Photosynthetic Rate comparison diagram after epiphysin;
Fig. 3 is under the conditions of lacking silicon and external source supplements tomato peel silicone content comparison diagram after epiphysin;
Fig. 4 is under the conditions of lacking silicon and external source supplements tomato pulp silicone content comparison diagram after epiphysin.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-4, a kind of embodiment provided by the invention: be technical solution in the embodiment of the present invention below into Row clearly and completely describes, it is clear that described embodiments are only a part of the embodiments of the present invention, rather than whole realities Apply example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without making creative work Every other embodiment, shall fall within the protection scope of the present invention.
Embodiment one
The application that epiphysin promotes silicon to absorb in tomato growth
(1) nursery: full, uniform tomato (Solanum lycopersicum L.) ' rouge and powder champion ' kind is chosen Son is chosen the neat seed that germinates and is sowed at equipped with tomato dedicated substrate (coco bran: mushroom slag: pearl using conventional method presoaking and germinating Rock=2V:2V:1V) in nursery.It is washed with deionized water root matrix after neat emergence, is trained using Hoagland nutrient solution It supports.It will wherein 1/2 seedling be put into the nutrient solution of scarce silicon (1/2Hoagland), remainder is put into normal Hoagland nutrition Liquid compares;
(2) it prepares epiphysin reagent: being the proportional arrangement added solvent of 5:3 first, in accordance with ethyl alcohol and methanol, then will take off black Element is dissolved with added solvent, finally carries out constant volume with mixed liquor of the distilled water to epiphysin and additive reagent, and prepare out concentration For the epiphysin reagent of 100 μm of ol/L and 150 μm of ol/L, and prepared epiphysin reagent is placed on to 4 DEG C or so of sealing ring Border is saved;
(3) spray: when growth of seedling to 21 heart of leaf, morning 08:00-10:00 spraying concentration is taking off for 100 μm of ol/L Melanocyte reagent was sprayed once every 3 days, is sprayed altogether 2 times, and until growth of seedling is bloomed to the first flower spike, selection is same again Sample time point spraying concentration is the epiphysin reagent of 150 μm of ol/L, sprayed 1 time, sprays 3 times every 5 days;
(4) leaf chlorophyll and Net Photosynthetic Rate detection: florescence 10:00-11:00 samples blade, takes under growing point the Five leaves measure tomato leaf chlorophyll content and net photosynthetic rate;
(5) silicone content detects: fruiting period, takes Tomato Root System, stalk, blade, pericarp, pulp separation drying, weighs drying Sample 0.3g is crushed, porcelain crucible is put into, 300 DEG C of pre- ashing 3h, are warming up to 550 DEG C of ashing 4h in Muffle furnace.Sample will be ashed With 48ml 0.08mol/L H2SO4It washes in clean polyethylene bottle, adds 2ml 40%HF, shaking table shaken overnight, filtering.It takes 2ml filtrate is transferred in new polyethylene bottle, and 50ml 0.32%H is added3BO3, vibrate 2h.10ml mixed liquor is taken, 5ml is added H2SO4Ammonium molybdate mixed liquor [H2SO4: ammonium molybdate 1:1 (v/v), H2SO4Concentration 0.08mol/L, (NH4)6Mo7O24·4H2O is dense Spend 20g/L], oscillating reactions 5min, is then added 3.3% tartaric acid of 5ml and 5ml 0.4% restores type ascorbic acid, and oscillation is anti- 25min is answered, absorbance is measured at 811nm, 3 repetitions calculate average value.
(6) according to above step measure under the conditions of scarce silicon and external source supplement epiphysin after Tomato Root System, stalk, blade silicon contain Amount comparison diagram is illustrated in fig. 1 shown below:
Note: with significant (P < 0.05) of lowercase letter indication difference different after column data in table
As can be seen from the figure spraying epiphysin to scarce silicon tomato can be improved the content of chlorophyll and the net light of tomato Rate is closed, promotes the absorption of element silicon during Development of Tomato, due to the promotion of tomato element silicon absorbability, plant fruit Silicone content increases in skin and pulp, so as to effectively prevent the diseases such as blossom-end rot to occur, improves the disease resistance ability of tomato, has Conducive to the growth and development of tomato.
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc. With replacement, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this Within the protection scope of invention.

Claims (5)

1. a kind of method for promoting tomato silicon to absorb with epiphysin, it is characterised in that: the following steps are included:
(1) with the epiphysin reagent that added solvent configuration concentration is 100-150 μm of ol/L;
(2) during 08:00-10:00, configured epiphysin reagent is sprayed on to tomato seedling blade face or the throwing of scarce silicon strip part It is added in the scarce silicon nutrient solution of Growth of Tomato Seedling.
2. according to claim 1 it is a kind of with epiphysin promote tomato silicon absorb method, it is characterised in that: it is described add it is molten Agent is that ethyl alcohol and methanol mixed configuration form, and mixed proportion is 5:3.
3. a kind of method for promoting tomato silicon to absorb with epiphysin according to claim 1, it is characterised in that: the epiphysin The configuration method of reagent is first to dissolve epiphysin with added solvent, the finally mixing with distilled water to epiphysin and additive reagent Liquid carries out constant volume.
4. a kind of method for promoting tomato silicon to absorb with epiphysin according to claim 1, it is characterised in that: the step (2) tomato seedling is respectively corresponded when tomato growth to 2 leaf, 1 heart and Growth of Tomato Seedling are bloomed to the first flower spike in spray it is dense Degree is the epiphysin reagent of 100 μm of ol/L and the epiphysin reagent of 150 μm of ol/L, and spraying number was 2-3 times, and every 3-5 days It sprays primary.
5. a kind of method for promoting tomato silicon to absorb with epiphysin described in claim 1 is on promoting Development of Tomato resistance Application.
CN201810603913.0A 2018-06-12 2018-06-12 Method for promoting tomato silicon absorption by melatonin and application Active CN109042665B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810603913.0A CN109042665B (en) 2018-06-12 2018-06-12 Method for promoting tomato silicon absorption by melatonin and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810603913.0A CN109042665B (en) 2018-06-12 2018-06-12 Method for promoting tomato silicon absorption by melatonin and application

Publications (2)

Publication Number Publication Date
CN109042665A true CN109042665A (en) 2018-12-21
CN109042665B CN109042665B (en) 2020-10-16

Family

ID=64820697

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810603913.0A Active CN109042665B (en) 2018-06-12 2018-06-12 Method for promoting tomato silicon absorption by melatonin and application

Country Status (1)

Country Link
CN (1) CN109042665B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679364A (en) * 2019-09-27 2020-01-14 昆明理工大学 Method for improving leaf disease resistance of panax notoginseng and application of method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104823738A (en) * 2015-04-13 2015-08-12 浙江大学 Application of melatonin in reducing cadmium accumulation in plants
CN105766410A (en) * 2016-03-16 2016-07-20 山东农业大学 Method for utilizing melatonin for promoting facility tomato fruit setting and growth
CN105900989A (en) * 2016-04-18 2016-08-31 浙江大学 Application of melatonin in improvement of absorption of sulfur by vegetables
CN107637598A (en) * 2017-09-26 2018-01-30 山东农业大学 Application of the epiphysin in promoting grafted tomato to survive and grow

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104823738A (en) * 2015-04-13 2015-08-12 浙江大学 Application of melatonin in reducing cadmium accumulation in plants
CN105766410A (en) * 2016-03-16 2016-07-20 山东农业大学 Method for utilizing melatonin for promoting facility tomato fruit setting and growth
CN105900989A (en) * 2016-04-18 2016-08-31 浙江大学 Application of melatonin in improvement of absorption of sulfur by vegetables
CN107637598A (en) * 2017-09-26 2018-01-30 山东农业大学 Application of the epiphysin in promoting grafted tomato to survive and grow

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BI-LI CAO等: "Effects of silicon on absorbed light allocation, antioxidant enzymesand ultrastructure of chloroplasts in tomato leaves under simulateddrought stress", 《SCIENTIA HORTICULTURAE》 *
丁飞: "褪黑素缓解番茄低温与水分胁迫机理研究", 《中国博士学位论文全文数据库 农业科技辑》 *
姜超强等: "褪黑素与植物抗逆性研究进展", 《生物技术通报》 *
巩彪 等: "园艺作物褪黑素的研究进展", 《中国农业科学》 *
杜天浩: "外源褪黑素对NaCl胁迫下番茄幼苗生理代谢和产量及品质的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
沈琼: "黄瓜果实表面蜡粉形成及其与硅吸收分配的关系研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110679364A (en) * 2019-09-27 2020-01-14 昆明理工大学 Method for improving leaf disease resistance of panax notoginseng and application of method
CN110679364B (en) * 2019-09-27 2022-03-25 昆明理工大学 Method for improving leaf disease resistance of panax notoginseng and application of method

Also Published As

Publication number Publication date
CN109042665B (en) 2020-10-16

Similar Documents

Publication Publication Date Title
CN105052655B (en) Oil tea implantation methods
CN106797861A (en) A kind of oil tea implantation methods
CN105379599A (en) High yield method for vigorous rootstock long-branch form apples
CN107372602A (en) A kind of rice growth regulator and preparation method thereof
CN110169346A (en) A kind of cherry and tomato soilless culture method of suitable torrid areas
CN103641636B (en) Nano light carbon agent and preparation method thereof
CN104938184A (en) Method for cultivating pakchoi which is highly rich in vitamin C
CN109042665A (en) A kind of method and application for promoting tomato silicon to absorb with epiphysin
CN114794113A (en) Use of 2-amino-3-phenylbutyric acid or derivatives thereof as plant growth regulators
CN110063194A (en) A kind of implantation methods of the multi-level fruit setting of citrus
CN106386343A (en) Seedling growing method for Callistemon rigidus
CN103070070A (en) Cultivation method of seedless roxburgh roses
CN109673654B (en) Application of progesterone as crop drought resistance agent and method for improving crop drought resistance
CN104322230A (en) Manual cultivation method of Chinese yew
CN114223543B (en) Cultivation method of weak light-resistant tomato variety
CN110521473A (en) The photosynthetic research method of the red oak in North America under a kind of raising drought stress
CN103782746A (en) Cultivation method of spraying exogenous material 6-BA on leaf surface to improve water logging resistance of cucumber
CN108713464A (en) A kind of low light tolerance tomato planting method
CN106550830A (en) The plantation of selenium-rich rice and new cultural method
CN107517836A (en) A kind of nutrient solution for gardens vegetable soilless culture and preparation method thereof
KR20040103146A (en) Cultivating process of Gombo-Baechu
CN106688556A (en) High-yield cultivation method of aralia elata seem in barren land
CN106538331A (en) A kind of hardening off method of black fruit lyceum plantlet in vitro
CN109793005A (en) Dormancy awakening chemical ongrape and the method for releasing grape winter buds dormancy ahead of time in facility
CN113854301B (en) Cabbage type rape seed initiator, initiation method and application

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant