CN109042072A - A kind of utilization is cracked rice the method for cultivating needle mushroom - Google Patents
A kind of utilization is cracked rice the method for cultivating needle mushroom Download PDFInfo
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- CN109042072A CN109042072A CN201811270192.2A CN201811270192A CN109042072A CN 109042072 A CN109042072 A CN 109042072A CN 201811270192 A CN201811270192 A CN 201811270192A CN 109042072 A CN109042072 A CN 109042072A
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- culture medium
- hydrogen phosphate
- needle mushroom
- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to field of biotechnology, and in particular to a kind of utilization is cracked rice the method for cultivating needle mushroom.This method includes culture medium preparation, spice, sterilizing, inoculation, bacterium germination and fruiting；Its culture medium prescription is as follows: include: by weight 20 parts crack rice, 27 ~ 33 portions of nutrient solutions；Wherein nutrient solution prescription is by mass percentage are as follows: glucose 2.0% ~ 2.5%, beef extract 0.4% ~ 0.6%, dipotassium hydrogen phosphate 0.18% ~ 0.22%, disodium hydrogen phosphate 0.14% ~ 0.19%, surplus are water, and the sum of each component is 100%, pH natural.Culture medium is covered with to foreshortening to the total time that former base is differentiated to form fructification 20 days or so using the Flammulina velutipes mycelium for culture medium cultivation of originally cracking rice, the Growth of Flammulina Velutipes period is greatly shortened compared with routine culture, it is simple to provide culture matrix manufacturing by the present invention simultaneously, it is easy to operate, it produces and processes at low cost, it can adapt to the factorial production and use, increase economic efficiency.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of utilization is cracked rice the method for cultivating needle mushroom.
Background technique
Needle mushroom also known as dried mushroom, plain mushroom, structure bacterium, cyanide, hair handle money bacterium, are subordinate to Basidiomycota, agaric guiding principle, agaric
Mesh steeps head Cordycepps, money Pseudomonas.Needle mushroom has higher nutritive value, medical value and health-care efficacy, is rich in protein, carbon
Hydrate, mineral element, vitamin and crude fibre, fat content is lower, and cholesterol-free, makees with numerous pharmacology
With, be more universal dual-purpose of drug and food fungi, have wide development prospect.The training used when the cultural method of needle mushroom and cultivation
Supporting base has important influence to the growth of needle mushroom.In traditional needle mushroom production process, cultural method frequently with solid spawn,
The speed of growth is slow, and the period is longer, and the formula of culture medium for cultivating is complicated, and complicated for operation, economic cost is high, and liquid spawn has life
Long fireballing feature, can be greatly improved production efficiency using this method.Due to the energy of needle mushroom decomposition of cellulose and lignin
Power is very strong, so all the time, mushroom grower is all using sawdust and cotton seed hulls as culturing raw material in production.But nowadays, " bacterium one
Woods " contradiction becomes increasingly conspicuous, and sawdust and cotton seed hulls price are also one to rise and rise again, and increases the cost of mushroom grower plantation needle mushroom, therefore
Each production unit is all attached great importance to look for cheap alternative materials.Contain a large amount of starch, protein, minerals in cracking rice
Etc. nutritional ingredients, be a kind of good edible fungus culturing raw material and needle mushroom cultivated with Broken rice as its raw material, avoid fructification by
The pollution residual to heavy metal and agriculture, the needle mushroom of cultivation is pollution-free, more trusts.
Summary of the invention
The purpose of the present invention is to provide a kind of production is simple, produce and process it is at low cost, it is full of nutrition, it is with short production cycle and
The golden mushroom plantation method of safety non-pollution is increased economic efficiency with reducing the production cost of needle mushroom.
In order to solve the above problem the present invention adopts the following technical scheme:
A kind of utilization is cracked rice the method for cultivating needle mushroom, and this method includes that culture medium preparation, spice, sterilizing, inoculation, bacterium germination core go out
Mushroom.
Its culture medium prescription is as follows: include: by weight 20 parts crack rice, 27 ~ 33 portions of nutrient solutions.Wherein nutrient solution prescription
By mass percentage are as follows: glucose 2.0% ~ 2.5%, beef extract 0.4% ~ 0.6%, dipotassium hydrogen phosphate 0.18% ~ 0.22%, phosphoric acid hydrogen
Disodium 0.14% ~ 0.19%, surplus are water, and the sum of each component is 100%, pH natural.
Specific implementation step is as follows:
(1) glucose of above-mentioned parts by weight, beef extract, dipotassium hydrogen phosphate, disodium hydrogen phosphate the preparation of culture medium: are weighed in water
It is configured to nutrient solution, weighs crack rice and be configured to golden mushroom plantation culture medium in proportion, 121 DEG C of sterilizings in high-pressure sterilizing pot
It is spare to take out cooling by 30min；
(2) it is inoculated with: being operated under gnotobasis, inoculum concentration 20%-25%；
(3) bacterium germination: it is protected from light culture 6-8d under 23-25 DEG C of environment, covers with matrix to mycelia and uncaps fruiting；
(4) management of producing mushroom: covering with culture material to mycelia, opens sealing cover and light, relative air humidity control 90%~95%,
Temperature regulates and controls at 8 DEG C~12 DEG C, with the illumination of 400~500 luxs or scattering light, reinforces ventilation, former base is promoted to be formed
And sporophore growth.
The present invention has the advantages that
1, green safe, from pollution, the speed of growth is fast.At this stage, the Main Cultivation raw material of needle mushroom is cotton seed hulls, wheat is bulging,
The agricultural residues such as corn stalk, there are the safety problems such as heavy metals exceeding standard and pesticide residue, are cultivated using it as raw material, due to life
Object enrichment may cause heavy metal and the residual content of agriculture in needle mushroom exceeded.The present invention cultivates gold with Broken rice as its raw material
Needle mushroom avoids the fructification pollution residual by heavy metal and agriculture.
2, nutrition-allocated proportion is reasonable, and it is fast to walk bacterium speed for liquid spawn inoculation.Using the golden mushroom for culture medium cultivation of originally cracking rice
Filament length expires culture medium to foreshortening to the total time that former base is differentiated to form fructification 14 days or so, improves production efficiency.
3, process flow is simple, is convenient for factory culture.Method cultivation needle mushroom eliminates traditional spice link,
To crack rice be added according to the ratio with nutrient solution in high-temperature resistance plastice box in 121 DEG C of progress steam sterilizings can and with plastic casing for hold
Device, eliminates the techniques such as loop-ringed bag-culture, mycelium stimulation, and process flow is simply easy to carry out large-scale planting production.
4, it plants needle mushroom compared with bag to compare, more aesthetically pleasing and culture medium is not easy to cause insect pest with Broken rice as its raw material, therefore, can
As and cultivation promoted with the needle mushroom potting that is integrated is watched.
Specific embodiment
Embodiment 1
Its culture medium prescription is as follows: addition is cracked rice and the nutrient solution of various composition of nutritive substances, and specific nutrient solution prescription presses quality
Percentages are as follows: glucose 2.0%, beef extract 0.4%, dipotassium hydrogen phosphate 0.22%, disodium hydrogen phosphate 0.19%, surplus are water, respectively
The sum of component is 100%, pH naturally, then in parts by weight, 27 portions of nutrient solutions are added in every 20 parts mixing of cracking rice.
Specific implementation step is as follows:
(1) glucose of above-mentioned parts by weight, beef extract, dipotassium hydrogen phosphate, disodium hydrogen phosphate the preparation of culture medium: are weighed in water
It is configured to nutrient solution, weighs crack rice and be configured to golden mushroom plantation culture medium in proportion, 121 DEG C of sterilizings in high-pressure sterilizing pot
It is spare to take out cooling by 30min；
(2) it is inoculated with: being operated under gnotobasis, inoculum concentration 20%；
(3) bacterium germination: it is protected from light culture 7d under 25 DEG C of environment, covers with matrix to mycelia and uncaps fruiting；
(4) management of producing mushroom: covering with culture material to mycelia, opens sealing cover and light, relative air humidity control 90%~95%,
Temperature regulates and controls at 8 DEG C~12 DEG C, with the illumination of 400~500 luxs or scattering light, reinforces ventilation, former base is promoted to be formed
And sporophore growth.
Embodiment 2
Its culture medium prescription is as follows: addition is cracked rice and the nutrient solution of various composition of nutritive substances, and specific nutrient solution prescription presses quality
Percentage calculates are as follows: glucose 2.5%, beef extract .6%, dipotassium hydrogen phosphate 0.18%, disodium hydrogen phosphate 0.14%, surplus are water, respectively
The sum of component is 100%, pH naturally, 30 portions of nutrient solutions then are added according to every 20 parts mixing of cracking rice.
Specific implementation step is as follows:
(1) glucose of above-mentioned parts by weight, beef extract, dipotassium hydrogen phosphate, disodium hydrogen phosphate the preparation of culture medium: are weighed in water
It is configured to nutrient solution, weighs crack rice and be configured to golden mushroom plantation culture medium in proportion, 121 DEG C of sterilizings in high-pressure sterilizing pot
It is spare to take out cooling by 30min；
(2) it is inoculated with: being operated under gnotobasis, inoculum concentration 25%；
(3) bacterium germination: it is protected from light culture 8d under 25 DEG C of environment, covers with matrix to mycelia and uncaps fruiting；
(4) management of producing mushroom: covering with culture material to mycelia, opens sealing cover and light, relative air humidity control 90%~95%,
Temperature regulates and controls at 8 DEG C~12 DEG C, with the illumination of 400~500 luxs or scattering light, reinforces ventilation, former base is promoted to be formed
And sporophore growth.
Embodiment 3
Addition is cracked rice and the nutrient solution of various composition of nutritive substances, specific nutrient solution prescription are calculated by mass percentage are as follows: grape
Sugared 2.0%, beef extract 0.5%, dipotassium hydrogen phosphate 0.20%, disodium hydrogen phosphate 0.19%, surplus are water, and the sum of each component is 100%,
PH is naturally, then in parts by weight, 33 portions of nutrient solutions are added in every 20 parts mixing of cracking rice.
Specific implementation step is as follows:
(1) glucose of above-mentioned parts by weight, beef extract, dipotassium hydrogen phosphate, disodium hydrogen phosphate the preparation of culture medium: are weighed in water
It is configured to nutrient solution, weighs crack rice and be configured to golden mushroom plantation culture medium in proportion, 121 DEG C of sterilizings in high-pressure sterilizing pot
It is spare to take out cooling by 30min；
(2) it is inoculated with: being operated under gnotobasis, inoculum concentration 22%；
(3) bacterium germination: it is protected from light culture 8d under 24 DEG C of environment, covers with matrix to mycelia and uncaps fruiting；
(4) management of producing mushroom: covering with culture material to mycelia, opens sealing cover and light, relative air humidity control 90%~95%,
Temperature regulates and controls at 8 DEG C~12 DEG C, with the illumination of 400~500 luxs or scattering light, reinforces ventilation, former base is promoted to be formed
And sporophore growth.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (3)
- A kind of method for cultivating needle mushroom 1. utilization is cracked rice, which is characterized in that this method include culture medium preparation, spice, sterilizing, Inoculation, bacterium germination and fruiting；Its culture medium prescription is as follows: include: by weight 20 parts crack rice, 27 ~ 33 portions of nutrient solutions；Wherein seek Nutrient solution component is by mass percentage are as follows: and glucose 2.0% ~ 2.5%, beef extract 0.4% ~ 0.6%, dipotassium hydrogen phosphate 0.18% ~ 0.22%, disodium hydrogen phosphate 0.14% ~ 0.19%, surplus are water, and the sum of each component is 100%, pH natural.
- The method for cultivating needle mushroom 2. a kind of utilization according to claim 1 is cracked rice, which is characterized in that step is embodied in it It is rapid as follows:(1) glucose of above-mentioned parts by weight, beef extract, dipotassium hydrogen phosphate, disodium hydrogen phosphate the preparation of culture medium: are weighed in water It is configured to nutrient solution, weighs crack rice and be configured to golden mushroom plantation culture medium in proportion, 121 DEG C of sterilizings in high-pressure sterilizing pot It is spare to take out cooling by 30min；(2) it is inoculated with: being operated under gnotobasis, Needle mushroom strain inoculum concentration is 20%-25%；(3) bacterium germination: it is protected from light culture 6-8d under 23-25 DEG C of environment, covers with matrix to mycelia and uncaps fruiting；(4) management of producing mushroom: covering with culture material to mycelia, opens sealing cover and light, relative air humidity control 90%~95%, Temperature regulates and controls at 8 DEG C~12 DEG C, with the illumination of 400~500 luxs or scattering light, reinforces ventilation, former base is promoted to be formed And sporophore growth.
- The method for cultivating needle mushroom 3. a kind of utilization according to claim 1 is cracked rice, which is characterized in that the step (2) In Needle mushroom strain be liquid spawn.
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| CN201811270192.2A CN109042072A (en) | 2018-10-29 | 2018-10-29 | A kind of utilization is cracked rice the method for cultivating needle mushroom |
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| CN201811270192.2A CN109042072A (en) | 2018-10-29 | 2018-10-29 | A kind of utilization is cracked rice the method for cultivating needle mushroom |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168613A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Fast manufacture method for liquid strain |
| CN103907476A (en) * | 2014-03-26 | 2014-07-09 | 上海惠芬果蔬专业合作社 | Method for bonsai-type cultivation of Coprinus comatus and culture mediums for cultivation of Coprinus comatus |
| CN106966774A (en) * | 2017-04-28 | 2017-07-21 | 福建农林大学 | A kind of method using cultivation flat mushroom of cracking rice |
| CN107996284A (en) * | 2017-12-28 | 2018-05-08 | 山东常生源菌业有限公司 | A kind of seafood mushroom production method |
-
2018
- 2018-10-29 CN CN201811270192.2A patent/CN109042072A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168613A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Fast manufacture method for liquid strain |
| CN103907476A (en) * | 2014-03-26 | 2014-07-09 | 上海惠芬果蔬专业合作社 | Method for bonsai-type cultivation of Coprinus comatus and culture mediums for cultivation of Coprinus comatus |
| CN106966774A (en) * | 2017-04-28 | 2017-07-21 | 福建农林大学 | A kind of method using cultivation flat mushroom of cracking rice |
| CN107996284A (en) * | 2017-12-28 | 2018-05-08 | 山东常生源菌业有限公司 | A kind of seafood mushroom production method |
Non-Patent Citations (1)
| Title |
|---|
| 田时炳等: "《蔬菜栽培技术》", 31 August 2018, 中国三峡出版社 * |
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Application publication date: 20181221 |
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