CN109042066A - Organic tremella cultivates technique - Google Patents
Organic tremella cultivates technique Download PDFInfo
- Publication number
- CN109042066A CN109042066A CN201811018177.9A CN201811018177A CN109042066A CN 109042066 A CN109042066 A CN 109042066A CN 201811018177 A CN201811018177 A CN 201811018177A CN 109042066 A CN109042066 A CN 109042066A
- Authority
- CN
- China
- Prior art keywords
- days
- room temperature
- tremella
- organic
- cultivates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of organic tremella cultivation technique, comprising the following steps: a, culture medium preparation；B, culture medium packs；C, sterilizing cooling；D, strain selects；E, strain is inoculated with；F, germination period culture；G, setting date culture；H, the puberty cultivates；I, be open bait；J, environmental Kuznets Curves；K, young ear period management；L, at ear period management；M, stop wet appearance；N, it harvests.The present invention can fully ensure that the nutrition in organic tremella cultivation by the selection to medium component；By the control to temperature and humidity in organic tremella cultivation, illumination and water, the growing environment of organic tremella is fully ensured that, increase yield rate.
Description
Technical field
The invention belongs to domestic fungus cultivating technical fields, and in particular to a kind of organic tremella cultivation technique.
Background technique
Organic tremella is a kind of common edible mushroom, since it can be enhanced human immunity, and enhancing tumor patient pair
The tolerance of radiation and chemotherapy, therefore pursued by the majority of consumers.Simultaneously as albumen rich in organic tremella
Matter vitamin, thus tremella powder also have the function of it is anti-ageing go wrinkle and Firm, often apply can also remove freckle, chloasma.With market
Demand it is increasing, the cultivation of organic tremella also becomes more and more important.Currently, common are machine tremella cultivates technical maturity
Tremella poor quality and yield it is also not high.Therefore, it is necessary to a kind of new cultivation techniques, to guarantee the quality and production of organic tremella
Amount.
Summary of the invention
The technical problems to be solved by the invention are in view of the above shortcomings of the prior art, to provide a kind of organic tremella training
Technique is planted, guarantees also to can guarantee yield while quality.
The technical scheme adopted by the invention is that: a kind of organic tremella cultivation technique, comprising the following steps:
A, preparation of culture medium: cotton seed hulls 88%, wheat bran 10%, land plaster 1% and sucrose 1% are mixed according to weight percent
Siccative is synthesized, and is mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantee pH value is 5.8-6.2；
B, culture medium packs: culture medium pre-temperature, and the low pressure that blender is packed into the ㎝ specification of 12.5cm × 55 after mixing evenly is poly-
Ethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
C, sterilizing cooling: after three sections of sterilizer sterilizings, chilling room cools；
D, strain selects: selection cell age -9 days 6 days, and the tremella strain that the produce agaric time is 15 days by the pure mycelia of tremella and is mixed
Raw bacterium incense ashes mycelia is spare after mixing evenly；
E, strain is inoculated with: in clean room, being accessed strain blend compounds cloth with 1 ㎝ inoculator of bore and is sealed pocket；
F, germination period culture: after inoculation in 3 days, 22 DEG C -25 DEG C of room temperature are controlled, keeps space relative humidity 50%；
G, setting date culture: -7 days 4 days after inoculation, 23 DEG C -25 DEG C of room temperature are controlled, keeps space relative humidity
60%；
H, the puberty cultivates: 8-10 days after inoculation, controlling 22 DEG C -23 DEG C of room temperature, keeps space relative humidity 60%；
I, be open bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade along pocket
Surrounding cuts 1 ㎝ of film；
J, environmental Kuznets Curves: after expanding cave, controlling 20 DEG C -22 DEG C of room temperature, keep space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
K, young ear period management: expand behind cave -5 days 4 days, control 22 DEG C -25 DEG C of room temperature, keep space relative humidity
85%-90% starts the micro- spray 72min of ultrasonic wave at interval of 3h-4h, at interval of 3h-4h ventilation 72min；
L, at ear period management: expand behind cave 15 days, controls 22 DEG C -25 DEG C of room temperature, start the micro- spray of ultrasonic wave at interval of 3h,
During water spray, it is always on illumination；
M, stop wet appearance: when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand by fructification center without agglomeration sense and table
Bright auricle be transferred to when all unfolding stopping water spray, control 25 DEG C -28 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
N, harvest: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, when fresh weight 150g-250g, Ji Kejin
Row harvesting.
In one embodiment, in the step a, by cotton seed hulls 78%, corncob 10%, wheat bran 10%, land plaster
1% and sucrose 1% be mixed into siccative according to weight percent.
In one embodiment, in step c, three sections of sterilizings are specific as follows:
First stage sterilising temp is 103 DEG C, sterilization time 1h, and second stage sterilising temp is 105 DEG C, sterilization time
For 1h, phase III sterilising temp is 105 DEG C, keeps the temperature 12h, enters chilling room cooling after shutting down 1h.
In one embodiment, in step f, 23 DEG C of room temperature are controlled.
In one embodiment, in step g, 24 DEG C of room temperature are controlled.
In one embodiment, in step h, 22.5 DEG C of room temperature are controlled.
In one embodiment, in step j, 21 DEG C of room temperature are controlled.
In one embodiment, in step k, 23 DEG C of room temperature are controlled, keeps space relative humidity 87%, at interval of
3.5h starts the micro- spray 72min of ultrasonic wave, at interval of 3.5h ventilation 72min.
In one embodiment, in step l, 23 DEG C of room temperature are controlled.
In one embodiment, in step m, 26 DEG C of room temperature are controlled.
The beneficial effects of the present invention are:
1, by the selection to medium component, the nutrition in organic tremella cultivation can be fully ensured that；
2, by the control to temperature and humidity in organic tremella cultivation, illumination and water, the life of organic tremella is fully ensured that
Long environment increases yield rate.
Specific embodiment
Below in conjunction with specific embodiment, invention is further described in detail.
Embodiment 1:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 88%, wheat bran 10%, land plaster 1% and sucrose 1% according to weight hundred
Divide than being mixed into siccative, and be mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantee pH value is 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 22 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 23 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 22 DEG C of room temperature, keep space relative humidity 60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 20 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 22 DEG C of room temperature, keep space relative humidity
85%, the micro- spray 72min of ultrasonic wave is started at interval of 3h, at interval of 3h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 22 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 25 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
Embodiment 2:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 88%, wheat bran 10%, land plaster 1% and sucrose 1% according to weight hundred
Divide than being mixed into siccative, and be mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantee pH value is 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 23 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 24 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 22.5 DEG C of room temperature, keep space relative humidity
60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 21 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 23 DEG C of room temperature, keep space relative humidity
87%, the micro- spray 72min of ultrasonic wave is started at interval of 3.5h, at interval of 3.5h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 23 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 26 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
Embodiment 3:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 88%, wheat bran 10%, land plaster 1% and sucrose 1% according to weight hundred
Divide than being mixed into siccative, and be mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantee pH value is 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 25 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 25 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 23 DEG C of room temperature, keep space relative humidity 60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 22 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 25 DEG C of room temperature, keep space relative humidity
90%, the micro- spray 72min of ultrasonic wave is started at interval of 4h, at interval of 4h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 25 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 28 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
Embodiment 4:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 78%, corncob 10%, wheat bran 10%, land plaster 1% and sucrose 1%
It is mixed into siccative according to weight percent, and is mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantees pH value
For 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 22 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 23 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 22 DEG C of room temperature, keep space relative humidity 60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 20 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 22 DEG C of room temperature, keep space relative humidity
85%, the micro- spray 72min of ultrasonic wave is started at interval of 3h, at interval of 3h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 22 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 25 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
Embodiment 5:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 78%, corncob 10%, wheat bran 10%, land plaster 1% and sucrose 1%
It is mixed into siccative according to weight percent, and is mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantees pH value
For 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 23 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 24 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 22.5 DEG C of room temperature, keep space relative humidity
60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 21 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 23 DEG C of room temperature, keep space relative humidity
87%, the micro- spray 72min of ultrasonic wave is started at interval of 3.5h, at interval of 3.5h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 23 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 26 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
Embodiment 6:
A kind of organic tremella cultivation technique, comprising the following steps:
Step 1: preparation of culture medium: by cotton seed hulls 78%, corncob 10%, wheat bran 10%, land plaster 1% and sucrose 1%
It is mixed into siccative according to weight percent, and is mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantees pH value
For 5.8-6.2；
Step 2: culture medium packs: culture medium pre-temperature, blender are packed into the low of the ㎝ specification of 12.5cm × 55 after mixing evenly
Pressure polyethylene forms bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
Step 3: sterilizing cooling: three sections of sterilizer sterilizings are sent into folded bag unwheeling, first stage sterilising temp is 103 DEG C,
Sterilization time is 1h, and second stage sterilising temp is 105 DEG C, sterilization time 1h, and phase III sterilising temp is 105 DEG C, is protected
Warm 12h enters chilling room cooling after shutting down 1h；
Step 4: strain selects: selection cell age -9 days 6 days, the tremella strain that the produce agaric time is 15 days, by the pure mycelia of tremella
Raw bacterium incense ashes mycelia is spare after mixing evenly with mixing；
Step 5: strain is inoculated with: in clean room, accessing strain blend compounds cloth with 1 ㎝ inoculator of bore and seal pocket；
Step 6: germination period culture: after inoculation in 3 days, controlling 25 DEG C of room temperature, keep space relative humidity 50%；
Step 7: setting date culture: -7 days 4 days after inoculation, controlling 25 DEG C of room temperature, keep space relative humidity
60%；
Step 8: the puberty cultivates: 8-10 days after inoculation, controlling 23 DEG C of room temperature, keep space relative humidity 60%；
Step 9: opening bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade edge
Pocket surrounding cuts 1 ㎝ of film；
Step 10: environmental Kuznets Curves: after expanding cave, 22 DEG C of room temperature are controlled, keeps space relative humidity 85%, at interval of
2min divulges information 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
Step 11: young ear period management: expanding behind cave -5 days 4 days, control 25 DEG C of room temperature, keep space relative humidity
90%, the micro- spray 72min of ultrasonic wave is started at interval of 4h, at interval of 4h ventilation 72min；
Step 12: at ear period management: expanding behind cave 15 days, control 25 DEG C of room temperature, it is micro- to start ultrasonic wave at interval of 3h
It sprays, during water spray, is always on illumination.
Step 13: stopping wet appearance: feeling by fructification center without agglomeration when fructification grows to 15 ㎝ of diameter, 8 ㎝ of thickness, hand
And show to be transferred to stopping water spray when auricle is all unfolded, control 28 DEG C of room temperature, keep space relative humidity 80% with
Under, at interval of 30min vented exhaust 20min, when operation, opens illumination；
Step 14: harvesting: inoculation -47 days or so 40 days, fructification diameter reach 10cm-15cm, fresh weight 150g-250g
When, it can be harvested.
In above-described embodiment, cotton seed hulls must select it is fresh, without go rotten it is rotten, without the agglomeration that drenches with rain, quality meets " edible mushroom
Culturing raw material cotton seed hulls " DB 35/T 1312-2013 standard, corncob can be cooperated in right amount, auxiliary material is preferably selected thick preferably wheat bran
Skin.
During tremella strain selects, there are certain association, comparing result such as tables for cell age, produce agaric number of days and survival rate
1:
1 tremella strain cell age of table influences produce agaric
It is above-mentioned it can be seen that cell age why the extracting rate time is slow within 4-5 days, survival rate is low, show that cell age is short, when inoculation due to
Mycelia mix it is cataclasm split, healing is slow, so hypoevolutism；And 10-13 days, the produce agaric time 14 days, survival rate was lower, showed mycelia
In aging, lose vigor.And 6-9 days produce agaric time was 15 days, survival rate is up to 98%, it follows that a best cell age is answered
Selection 6-9 days, advantageous optimum period produce agaric improve survival rate.
The purpose of tremella strain stirring strain is that the pure mycelia of tremella is allowed to be uniformly mixed with raw bacterium incense ashes mycelia is mixed, such produce agaric
Rate is high, otherwise will appear only long mycelia, loses produce agaric.
18 days or so after inoculation, opened pocket adhesive plaster was originally sealed after inoculation, and cut 1 ㎝ of film along pocket surrounding with blade,
Make in outside air osmotic bag.It tears off the seal after inoculation and expands the number of days in cave and affect produce agaric rate, comparing result such as table 2:
Table 2, which tears off the seal, to be expanded the influence of cave time and produce agaric and compares
Wherein, the time: refer to days post inoculation；Pocket performance: to expand cave 24 hours as standard；Produce agaric rate is to expand behind cave as mark
It is quasi-.
Mycelium inoculation external world oxygen after flaring accelerates metabolism, and long speed is accelerated, and bacterium temperature is 2 DEG C -3 higher than room temperature in bag
℃；Therefore the indoor limit is not less than 18 DEG C, not higher than 23 DEG C only, avoids room temperature too high or too low and causes and spit brilliant droplet or black
Droplet, the ultimate rotten base of a fruit.At ear phase room temperature not less than 20 DEG C and not higher than 28 DEG C.
Organic tremella that this technique is cultivated is generally 45 days or so from harvesting is seeded to, fructification maturity symbol when mature
Are as follows: auricle all stretches, and performance is loose, stops growing, does not have microtia stamen；It is similar to peony or chrysanthemum, color is fresh white or cream-coloured,
It is slightly elastic.Fructification diameter is up to 10cm -15cm, fresh weight 150g -250g, after fructification is mature, can give out a large amount of white
Color basidiospore.In addition it is also possible to be determined according to the weight of bacterium bag；Full ripe fructification, bacterium bag are held in the hand very gently,
Show that nutrient has been decomposed to run out of.Reaching this standard should harvest in time.
A specific embodiment of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (10)
1. a kind of organic tremella cultivates technique, it is characterised in that: the following steps are included:
A, preparation of culture medium: cotton seed hulls 88%, wheat bran 10%, land plaster 1% and sucrose 1% are mixed into according to weight percent dry
Material, and it is mixed into culture medium according to the weight ratio of siccative and water 1:1.1-1.2, guarantee pH value is 5.8-6.2；
B, culture medium packs: culture medium pre-temperature, blender are packed into the low pressure polyethylene of the ㎝ specification of 12.5cm × 55 after mixing evenly
Form bag, per it is packed enter siccative 0.7kg, being added after water and controlling its weight is 1.6kg, is packed into folded bag unwheeling；
C, sterilizing cooling: after three sections of sterilizer sterilizings, chilling room cools；
D, strain selects: selection cell age -9 days 6 days, and the tremella strain that the produce agaric time is 15 days by the pure mycelia of tremella and mixes raw bacterium
Incense ashes mycelia is spare after mixing evenly；
E, strain is inoculated with: in clean room, being accessed strain blend compounds cloth with 1 ㎝ inoculator of bore and is sealed pocket；
F, germination period culture: after inoculation in 3 days, 22 DEG C -25 DEG C of room temperature are controlled, keeps space relative humidity 50%；
G, setting date culture: -7 days 4 days after inoculation, 23 DEG C -25 DEG C of room temperature are controlled, keeps space relative humidity 60%；
H, the puberty cultivates: 8-10 days after inoculation, controlling 22 DEG C -23 DEG C of room temperature, keeps space relative humidity 60%；
I, be open bait: -18 days 12 days after inoculation, the adhesive plaster that pocket is sealed after inoculation being opened, and with blade along pocket surrounding
Cut 1 ㎝ of film；
J, environmental Kuznets Curves: after expanding cave, controlling 20 DEG C -22 DEG C of room temperature, keep space relative humidity 85%, logical at interval of 2min
Wind 1 time, every 3min illumination 1 time, illuminance 100LX-200LX；
K, young ear period management: expanding behind cave -5 days 4 days, control 22 DEG C -25 DEG C of room temperature, keep space relative humidity 85%-90%,
The micro- spray 72min of ultrasonic wave is started at interval of 3h-4h, at interval of 3h-4h ventilation 72min；
L, at ear period management: expanding behind cave 15 days, control 22 DEG C -25 DEG C of room temperature, start the micro- spray of ultrasonic wave at interval of 3h, spray water
In the process, it is always on illumination；
M, stop wet appearance: when fructification grow to 15 ㎝ of diameter, 8 ㎝ of thickness, hand by fructification center without agglomeration feel and show ear
It is transferred to stopping water spray when piece is all unfolded, controls 25 DEG C -28 DEG C of room temperature, keeps space relative humidity 80% hereinafter, every
Illumination is opened when 30min vented exhaust 20min, operation；
N, harvest: inoculation -47 days or so 40 days, fructification diameter reaches 10cm-15cm, when fresh weight 150g-250g, can be adopted
It receives.
2. organic tremella according to claim 1 cultivates technique, it is characterised in that:, can also be by cotton seed hulls in the step a
78%, corncob 10%, wheat bran 10%, land plaster 1% and sucrose 1% are mixed into siccative according to weight percent.
3. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step c, three sections of sterilizings are specific
It is as follows:
First stage sterilising temp is 103 DEG C, sterilization time 1h, and second stage sterilising temp is 105 DEG C, and sterilization time is
1h, phase III sterilising temp are 105 DEG C, keep the temperature 12h, enter chilling room cooling after shutting down 1h.
4. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step f, control room temperature 23
℃。
5. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step g, control room temperature 24
℃。
6. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step h, control room temperature 22.5
℃。
7. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step j, control room temperature 21
℃。
8. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step k, control room temperature 23
DEG C, space relative humidity 87% is kept, the micro- spray 72min of ultrasonic wave is started at interval of 3.5h, at interval of 3.5h ventilation 72min.
9. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step l, control room temperature 23
℃。
10. organic tremella according to claim 1 cultivates technique, it is characterised in that: in step m, control room temperature 26
℃。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811018177.9A CN109042066A (en) | 2018-09-03 | 2018-09-03 | Organic tremella cultivates technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811018177.9A CN109042066A (en) | 2018-09-03 | 2018-09-03 | Organic tremella cultivates technique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109042066A true CN109042066A (en) | 2018-12-21 |
Family
ID=64758268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811018177.9A Pending CN109042066A (en) | 2018-09-03 | 2018-09-03 | Organic tremella cultivates technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109042066A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754296A (en) * | 2019-12-19 | 2020-02-07 | 大连森源菌业有限公司 | Tremella fuciformis cultivation method |
| CN111670749A (en) * | 2020-07-09 | 2020-09-18 | 福建天天源生物科技有限公司 | Herbal tremella planting method |
| CN113711850A (en) * | 2021-09-18 | 2021-11-30 | 四川工大西南食品研究有限责任公司 | Tremella fuciformis cultivation method, tremella fuciformis, culture medium and application |
| CN114451218A (en) * | 2022-02-08 | 2022-05-10 | 福建信龙农产品开发有限公司 | Tremella fuciformis cultivation material and tremella fuciformis cultivation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972210A (en) * | 2012-12-12 | 2013-03-20 | 张德品 | New tremella cultivation method |
| CN103535184A (en) * | 2013-08-24 | 2014-01-29 | 江剑峰 | White fungus cultivation method |
| CN106489526A (en) * | 2016-10-27 | 2017-03-15 | 浙江瑞丰农业开发股份有限公司 | A kind of industrially cultivating method of Tremella |
| CN107711293A (en) * | 2017-10-31 | 2018-02-23 | 广西吉朋投资有限公司 | A kind of white fungus compost and preparation method |
| CN108370842A (en) * | 2016-11-17 | 2018-08-07 | 艾光海 | A kind of cultivating white fungus method |
-
2018
- 2018-09-03 CN CN201811018177.9A patent/CN109042066A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972210A (en) * | 2012-12-12 | 2013-03-20 | 张德品 | New tremella cultivation method |
| CN103535184A (en) * | 2013-08-24 | 2014-01-29 | 江剑峰 | White fungus cultivation method |
| CN106489526A (en) * | 2016-10-27 | 2017-03-15 | 浙江瑞丰农业开发股份有限公司 | A kind of industrially cultivating method of Tremella |
| CN108370842A (en) * | 2016-11-17 | 2018-08-07 | 艾光海 | A kind of cultivating white fungus method |
| CN107711293A (en) * | 2017-10-31 | 2018-02-23 | 广西吉朋投资有限公司 | A kind of white fungus compost and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 丁湖广 丁荣辉: "《名优特色菇耳栽培新法》", 31 August 2004 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754296A (en) * | 2019-12-19 | 2020-02-07 | 大连森源菌业有限公司 | Tremella fuciformis cultivation method |
| CN111670749A (en) * | 2020-07-09 | 2020-09-18 | 福建天天源生物科技有限公司 | Herbal tremella planting method |
| CN113711850A (en) * | 2021-09-18 | 2021-11-30 | 四川工大西南食品研究有限责任公司 | Tremella fuciformis cultivation method, tremella fuciformis, culture medium and application |
| CN114451218A (en) * | 2022-02-08 | 2022-05-10 | 福建信龙农产品开发有限公司 | Tremella fuciformis cultivation material and tremella fuciformis cultivation method |
| CN114451218B (en) * | 2022-02-08 | 2023-07-25 | 福建信龙农产品开发有限公司 | Tremella cultivation material and tremella cultivation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109042066A (en) | Organic tremella cultivates technique | |
| CN104285678B (en) | A kind of method of flat mushroom Super-high-yielding Cultivation | |
| CN102318505B (en) | Ganoderma lucidum potted landscape cultivation method | |
| CN103907478B (en) | The method that flat mushroom bonsai type is cultivated and the substratum for cultivating white mushroom | |
| CN104920067A (en) | High-yield black fungus cultivating method | |
| CN105237090B (en) | Phellinus solid state bacterial turns the breeding method of liquid culture medium | |
| CN102090266B (en) | High-efficient cultivation method of schizophyllum commune | |
| CN102863273B (en) | Field cultivating black fungus culture medium in Tibet region and cultivation method for field cultivating black fungus in Tibet region | |
| CN104160872B (en) | A kind of method for planting almond abalone mushroom of safety and environmental protection | |
| CN106083394A (en) | A kind of method utilizing reed powder to make Enoki mushroom cultivation material | |
| CN104303840B (en) | A kind of cultural method of dish dress Flammulina velutiper (Fr.) Sing | |
| CN109997608A (en) | A kind of implantation methods of edible mushroom | |
| CN106718028A (en) | A kind of golden ear high-yield seeds production cultural method | |
| CN106747848A (en) | South Korea's Phellinus bacterium plastic bag cultivation method | |
| CN103044126A (en) | Lentinus edodes cultivation material and making method thereof | |
| CN106718045A (en) | A kind of method for cultivating mushroom | |
| CN106576905A (en) | Shiitake mushroom cultivation method | |
| CN105875199A (en) | High-and-stable-yield cultivation method for Schizophyllumcommuneh | |
| CN106242824A (en) | A kind of planting almond abalone mushroom material and use this planting material produce Pleurotus eryngii implantation methods | |
| CN106754409B (en) | Method for cultivating and planting holothuria leucorrhoeae | |
| CN111149619B (en) | Cultivation method of pholiota nameko | |
| CN108739060A (en) | A method of the branch mushroom culture that matrimony vine powder, Chinese wolfberry carpopodium fruit leaf and the trimming process generated with matrimony vine processing generates | |
| CN107646523A (en) | A kind of cultural method of mushroom with abundant selenium | |
| CN105359836B (en) | Fruiting method is induced during wild matsutake artificial cultivation | |
| CN110291922A (en) | Breeding method is planted in a kind of high protein tremella room |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181221 |