CN109007139A - A kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method - Google Patents
A kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method Download PDFInfo
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- CN109007139A CN109007139A CN201810618156.4A CN201810618156A CN109007139A CN 109007139 A CN109007139 A CN 109007139A CN 201810618156 A CN201810618156 A CN 201810618156A CN 109007139 A CN109007139 A CN 109007139A
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- selenium
- selenoprotein
- plant
- peptide
- selenoaminoacid
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention provides a kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method, selects selenium-enriched tea leaf, tea tree branch, is cleaned with pure water；It is crushed with pulverizer, selenium-rich particle is made；Selenium-rich particle is cooled to 16 DEG C -18 DEG C, grinds and selenium-enriched plant sauce is made, pure water is added in selenium-enriched plant sauce and is stirred 30 minutes in low temperature blender, dissolves it sufficiently, selenium-enriched plant slurry is then obtained by filtering；Edible ethanol is added in the plant selenoprotein produced, hybrid filtering obtains the selenoprotein of purity is high, high purity selenium albumen obtained is dissolved under alkaline condition, be added alkali protease be mixed evenly, 32 DEG C -36 DEG C of constant temperature 40 minutes, by aqueous selenium peptide selenoaminoacid mixture be put into freeze dryer be lyophilized 50 minutes, it is dehydrated to 10%, dry product selenium peptide selenoaminoacid is obtained, organic selenium peptide selenium egg amino acid is finally extracted, present invention process is simple, manufacture craft is simple, is suitable for promoting the use of.
Description
Technical field
The present invention relates to a kind of raw material extracting method more particularly to a kind of organic seleniums, the mixing of selenoprotein selenium peptide selenoaminoacid
Object extracting method.
Background technique
Selenium is trace mineral nutrient needed by human.It is thought that selenium is Harmful Trace Elements in the past, but card now
It is a kind of indispensable element of human body in fact.Selenium and vitamin E are both antioxidant, also turn out that it draws to protein is lacked
The underfed children risen have therapeutic effect, have resistant function to certain possible carcinogens.People is slight or moderate lacks
Selenium, sign or symptom are unobvious.The issuable disease of human body selenium deficiency: energy deficiency malnutrition, blood hemolytic anemia, gram
Mountain disease, Kaschin-Beck disease, hypertension, ischemic heart disease, cirrhosis, pancreatitis, fibroma, cancer, myomata, not pregnant disease, glycosuria
Disease, cataract etc.；The intake of organic selenium has the advantage that in life
(1), removing toxic substances, gas defence, antipollution, remove bodily waste, garbage toxins, can mitigate chemical carcinogen, pesticide and
The toxic side effect of cancer object is connect, selenium is natural antidote；
(2), selenium improves red blood cell oxygen carrying capacity, and the hemalbumin of the haemocyte in blood, red blood cell is protected to will not be oxidized,
Its oxygen carrying capacity is just strong, solves the problems, such as the cerebral anoxia of brain worker, reduces the fatigue phenomenon of depression；
(3), the advantage with protection eyes, prevention and treatment eye illness and cancer-resisting；
(4), liver is protected, appropriate selenium-supply can finally remove about 90% hepatitis B, and appropriate selenium-supply can play
Acceleration of alcohol catabolism, and then protect liver；
(5), it delays senescence, selenium cooperates vitamin E, can play stronger antioxidation, effective delaying cell aging；Also
It can safeguard that male reproductive system, selenium are known as gold in males, male reproductive system selenium content accounts for human body selenium content
20%-40%, selenium are important nutrition and the male reproductive system immune function element of Sperm specific enzyme metabolism；
(6), pancreas islet is protected, selenium deficiency can cause the primary lesion of some islet cells synthesis and secretin function, selenium-supply
Pancreas islet can be protected；Selenium can manage property hands at the back sufficient edema, allevating angina pectoris, reduce blood pressure.
By experimental studies have found that, the plant organic selenium rate of being absorbed by the body is 20 times of inorganic selenium.Through clinical test: organic
Selenium is largely taken in harmless for a long time, and organic selenium can monthly take in 500-1000ug, and inorganic selenium is largely taken in and will be occurred
Selenosis.Developed country has not had to nutritional supplement of the simple inorganic salt form as selenium, and the developed countries such as Japan, U.S. are
Forbidden adding the inorganic seleniums such as sodium selenite in food.And the domestic biological the technique of extracting about organic selenium is opposite at present
It is less, so, a kind of economical rationality, the organic selenium of function admirable, selenoprotein selenium peptide selenoaminoacid mixture extracting method become
Entire society's urgent problem to be solved.
Summary of the invention
To solve the above problems, the present invention provides a kind of organic selenium, selenoprotein selenium peptide selenoaminoacid mixture extracting method.
The present invention provides a kind of organic selenium, selenoprotein selenium peptide selenoaminoacid mixture extracting method, comprising the following steps:
1) removal insect pest, withered, disease, inferior fresh selenium-enriched tea leaf and tea tree branch are selected, it will be fresh with pure water
Selenium-enriched tea leaf and tea tree branch wash clean, after moisture drains, are made selenium-rich particle；
2) after selenium-rich particle obtained in step (1) being cooled to 16 DEG C -18 DEG C, low speed colloid mill grinding butter, system are then used
Obtain selenium-enriched plant sauce；
3) pure water with selenium-enriched plant sauce homogenous quantities is added in selenium-enriched plant sauce obtained in step (2), is put into low temperature
30 minutes are stirred in blender to abundant dissolution, obtain selenium-enriched plant mixed liquor；
4) after with gauze filter to selenium-enriched plant mixed liquor obtained in step (3) filtering, selenium-enriched plant slurry is obtained；
5) cellulase is added in step (4) in selenium-enriched plant obtained slurry and is uniformly mixed, is put into stainless steel barrel
After standing 20min, TG enzyme is added in stainless steel barrel, stirring evenly makes 34 DEG C -36 DEG C of constant temperature in bucket, wait react 30 minutes, side
For the reaction of stirring side until cellulose sufficiently decomposes, mixture A is made in proteins coagulation；
6) carbodiimides that selenium-enriched plant starches 2/1000ths weight is added made from step (5) in mixture A, makes egg
White matter dehydration, is come out Separation of Proteins using molecule bag filter, obtains plant selenoprotein；
7) edible ethanol of equal quality is added in the plant selenoprotein obtained into step (6), makes its full and uniform mixing,
Make protein precipitation, obtains sediment A；Separation of Proteins is come by sediment A with molecule bag filter, high-purity can be obtained
Plant selenoprotein；
8) after two centesimal sodium bicarbonates being added in plant selenoprotein made from step (7), 2 times of plants are added
The pure water of object selenoprotein is completely dissolved plant selenoprotein under alkaline condition, is subsequently added into the thousand of Selenium in Plants albumen quality
/ tri- alkali proteases are mixed evenly, 32 DEG C -36 DEG C of constant temperature 40 minutes；Until plant selenoprotein digests completely, obtain
Obtain mixed liquid B；
9) mixed liquid B in step (8) is heated to 80 DEG C until having selenium peptide and selenoaminoacid to be precipitated completely from mixed liquid B
Until, water salt, selenium peptide and selenoaminoacid will be separated with molecule bag filter, and obtain mixture C；
10) mixed liquor C obtained in step (9) is put into 50 minutes in freeze dryer and is lyophilized, be dehydrated to 10%, can be obtained
Organic selenium peptide selenium egg amino acid.
Further, the selenium-rich particle is the 1mm particle that production is crushed by double-pole pulverizer.
Further, the quality of the cellulase in the step (5) accounts for thousand points of selenium-enriched plant slurry quality in step (4)
Three, the quality of the TG enzyme in the step (5) accounts for 6/1000ths of selenium-enriched plant slurry quality in step (4).
Further, the mixture C is the mixture of water salt, selenium peptide and selenoaminoacid.
Further, the organic selenium peptide selenium egg amino acid in the step (10) is the mixing of selenoprotein and selenomethionine
Object.
Beneficial effects of the present invention:
Organic selenium of the invention, selenoprotein selenium peptide selenoaminoacid mixture are mentioned from fresh selenium-enriched tea leaf and tea tree branch
It takes, cost of manufacture is cheap, manufacture craft is simple, can produce on a large scale, is suitable for promoting the use of, solves organic in traditional technology
The problems such as extraction of selenium is difficult.
Specific embodiment
The present invention will be further described combined with specific embodiments below.
Embodiment 1: the present embodiment provides a kind of organic seleniums, selenoprotein selenium peptide selenoaminoacid mixture extracting method, including
Following steps:
1) removal insect pest, withered, disease, inferior fresh selenium-enriched tea leaf and tea tree branch are selected, it will be fresh with pure water
Selenium-enriched tea leaf and tea tree branch wash clean, after moisture drains, are made selenium-rich particle；
2) after selenium-rich particle obtained in step (1) being cooled to 16 DEG C -18 DEG C, low speed colloid mill grinding butter, system are then used
Obtain selenium-enriched plant sauce；
3) pure water with selenium-enriched plant sauce homogenous quantities is added in selenium-enriched plant sauce obtained in step (2), is put into low temperature
30 minutes are stirred in blender to abundant dissolution, obtain selenium-enriched plant mixed liquor；
4) after with gauze filter to selenium-enriched plant mixed liquor obtained in step (3) filtering, selenium-enriched plant slurry is obtained；
5) cellulase is added in step (4) in selenium-enriched plant obtained slurry and is uniformly mixed, is put into stainless steel barrel
After standing 20min, TG enzyme is added in stainless steel barrel, stirring evenly makes 34 DEG C -36 DEG C of constant temperature in bucket, wait react 30 minutes, side
For the reaction of stirring side until cellulose sufficiently decomposes, mixture A is made in proteins coagulation；
6) carbodiimides that selenium-enriched plant starches 2/1000ths weight is added made from step (5) in mixture A, makes egg
White matter dehydration, is come out Separation of Proteins using molecule bag filter, obtains plant selenoprotein；
7) edible ethanol of equal quality is added in the plant selenoprotein obtained into step (6), makes its full and uniform mixing,
Make protein precipitation, obtains sediment A；Separation of Proteins is come by sediment A with molecule bag filter, high-purity can be obtained
Plant selenoprotein；
8) after two centesimal sodium bicarbonates being added in plant selenoprotein made from step (7), 2 times of plants are added
The pure water of object selenoprotein is completely dissolved plant selenoprotein under alkaline condition, is subsequently added into the thousand of Selenium in Plants albumen quality
/ tri- alkali proteases are mixed evenly, 32 DEG C -36 DEG C of constant temperature 40 minutes；Until plant selenoprotein digests completely, obtain
Obtain mixed liquid B；
9) mixed liquid B in step (8) is heated to 80 DEG C until having selenium peptide and selenoaminoacid to be precipitated completely from mixed liquid B
Until, water salt, selenium peptide and selenoaminoacid will be separated with molecule bag filter, and obtain mixture C；
10) mixed liquor C obtained in step (9) is put into 50 minutes in freeze dryer and is lyophilized, be dehydrated to 10%, can be obtained
Organic selenium peptide selenium egg amino acid.
The selenium-rich particle is the 1mm particle that production is crushed by double-pole pulverizer.Cellulase in the step (5)
Quality account for 3/1000ths of the slurry quality of selenium-enriched plant in step (4), the quality of the TG enzyme in the step (5) accounts for step (4)
6/1000ths of middle selenium-enriched plant slurry quality.The mixture C is the mixture of water salt, selenium peptide and selenoaminoacid, the step
(10) the organic selenium peptide selenium egg amino acid in is the mixture of selenoprotein and selenomethionine.
The present invention and its embodiments have been described above, this description is not restricted.All in all if originally
The those of ordinary skill in field is enlightened by it, without departing from the spirit of the invention, is not inventively designed
Frame mode similar with the technical solution and embodiment, are within the scope of protection of the invention.
Claims (5)
1. a kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method, which is characterized in that including following step
It is rapid:
1) removal insect pest, withered, disease, inferior fresh selenium-enriched tea leaf and tea tree branch are selected, with pure water by fresh selenium-rich
Tealeaves and tea tree branch wash clean, after moisture drains, are made selenium-rich particle；
2) after selenium-rich particle obtained in step (1) being cooled to 16 DEG C -18 DEG C, low speed colloid mill grinding butter is then used, richness is made
Selenium plant paste；
3) pure water with selenium-enriched plant sauce homogenous quantities is added in selenium-enriched plant sauce obtained in step (2), is put into low temperature stirring
30 minutes are stirred in machine to abundant dissolution, obtain selenium-enriched plant mixed liquor；
4) after with gauze filter to selenium-enriched plant mixed liquor obtained in step (3) filtering, selenium-enriched plant slurry is obtained；
5) cellulase is added in step (4) in selenium-enriched plant obtained slurry and is uniformly mixed, be put into stainless steel barrel and stand
After 20min, TG enzyme is added in stainless steel barrel, stirring evenly makes 34 DEG C -36 DEG C of constant temperature in bucket, wait react 30 minutes, side stirring
Until cellulose sufficiently decomposes, mixture A is made in proteins coagulation for side reaction；
6) carbodiimides that selenium-enriched plant starches 2/1000ths weight is added made from step (5) in mixture A, makes protein
Dehydration, is come out Separation of Proteins using molecule bag filter, obtains plant selenoprotein；
7) edible ethanol of equal quality is added in the plant selenoprotein obtained into step (6), makes its full and uniform mixing, makes egg
White matter precipitating, obtains sediment A；Separation of Proteins is come by sediment A with molecule bag filter, the plant of high-purity can be obtained
Object selenoprotein；
8) after two centesimal sodium bicarbonates being added in plant selenoprotein made from step (7), 2 times of Selenium in Plants are added
The pure water of albumen is completely dissolved plant selenoprotein under alkaline condition, be subsequently added into Selenium in Plants albumen quality thousand/
Three alkali proteases are mixed evenly, 32 DEG C -36 DEG C of constant temperature 40 minutes；Until plant selenoprotein digests completely, mixed
Close liquid B；
9) mixed liquid B in step (8) is heated to 80 DEG C is up to having selenium peptide and selenoaminoacid to be precipitated completely from mixed liquid B
Only, water salt, selenium peptide and selenoaminoacid will be separated with molecule bag filter, and will obtain mixture C；
10) mixed liquor C obtained in step (9) is put into 50 minutes in freeze dryer and is lyophilized, be dehydrated to 10%, can be obtained organic
Selenium peptide selenium egg amino acid.
2. a kind of organic selenium according to claim 1, selenoprotein selenium peptide selenoaminoacid mixture extracting method, feature exist
In the selenium-rich particle is the 1mm particle for crushing production by double-pole pulverizer.
3. a kind of organic selenium according to claim 1, selenoprotein selenium peptide selenoaminoacid mixture extracting method, feature exist
In the quality of the cellulase in the step (5) accounts for 3/1000ths of selenium-enriched plant slurry quality in step (4), the step
(5) quality of the TG enzyme in accounts for 6/1000ths of selenium-enriched plant slurry quality in step (4).
4. a kind of organic selenium according to claim 1, selenoprotein selenium peptide selenoaminoacid mixture extracting method, feature exist
In the mixture C is the mixture of water salt, selenium peptide and selenoaminoacid.
5. a kind of organic selenium according to claim 1, selenoprotein selenium peptide selenoaminoacid mixture extracting method, feature exist
In the organic selenium peptide selenium egg amino acid in the step (10) is the mixture of selenoprotein and selenomethionine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810618156.4A CN109007139A (en) | 2018-06-15 | 2018-06-15 | A kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810618156.4A CN109007139A (en) | 2018-06-15 | 2018-06-15 | A kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method |
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| CN201810618156.4A Withdrawn CN109007139A (en) | 2018-06-15 | 2018-06-15 | A kind of organic selenium, selenoprotein and selenium peptide selenoaminoacid mixture extracting method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909251A (en) * | 2020-08-14 | 2020-11-10 | 江西省农业科学院农产品质量安全与标准研究所 | Rice selenium-rich gene OsHSE2-1 and application thereof |
| CN112841658A (en) * | 2021-01-25 | 2021-05-28 | 安徽硒瑞恩生物科技有限公司 | Nucleic acid selenium compound and preparation method thereof |
| CN113142587A (en) * | 2021-04-21 | 2021-07-23 | 余明朝 | Tea selenium formula |
| CN113968649A (en) * | 2021-10-20 | 2022-01-25 | 林欣 | Selenium-enriched drinking water and preparation method thereof |
| CN113975367A (en) * | 2021-08-02 | 2022-01-28 | 周子人 | Red zero-valent elemental nano-selenium oral liquid and preparation method and application thereof |
| CN114271372A (en) * | 2021-09-05 | 2022-04-05 | 江苏中农科食品工程股份有限公司 | Preparation method of plant selenoprotein product with hyaluronic acid as carrier |
| CN115843917A (en) * | 2022-11-30 | 2023-03-28 | 湖北硒盟健康科技有限公司 | Extraction method and application of selenoprotein and selenopeptide in cardamine violifolia |
-
2018
- 2018-06-15 CN CN201810618156.4A patent/CN109007139A/en not_active Withdrawn
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909251A (en) * | 2020-08-14 | 2020-11-10 | 江西省农业科学院农产品质量安全与标准研究所 | Rice selenium-rich gene OsHSE2-1 and application thereof |
| CN111909251B (en) * | 2020-08-14 | 2021-10-22 | 江西省农业科学院农产品质量安全与标准研究所 | Rice selenium-rich gene OsHSE2-1 and application thereof |
| CN112841658A (en) * | 2021-01-25 | 2021-05-28 | 安徽硒瑞恩生物科技有限公司 | Nucleic acid selenium compound and preparation method thereof |
| CN113142587A (en) * | 2021-04-21 | 2021-07-23 | 余明朝 | Tea selenium formula |
| CN113975367A (en) * | 2021-08-02 | 2022-01-28 | 周子人 | Red zero-valent elemental nano-selenium oral liquid and preparation method and application thereof |
| CN114271372A (en) * | 2021-09-05 | 2022-04-05 | 江苏中农科食品工程股份有限公司 | Preparation method of plant selenoprotein product with hyaluronic acid as carrier |
| CN114271372B (en) * | 2021-09-05 | 2024-04-02 | 江苏中农科食品工程股份有限公司 | Preparation method of plant selenoprotein product with hyaluronic acid as carrier |
| CN113968649A (en) * | 2021-10-20 | 2022-01-25 | 林欣 | Selenium-enriched drinking water and preparation method thereof |
| CN115843917A (en) * | 2022-11-30 | 2023-03-28 | 湖北硒盟健康科技有限公司 | Extraction method and application of selenoprotein and selenopeptide in cardamine violifolia |
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Application publication date: 20181218 |