CN108977389A - A method of killing wheel animalcule in process of culturing spirulina - Google Patents
A method of killing wheel animalcule in process of culturing spirulina Download PDFInfo
- Publication number
- CN108977389A CN108977389A CN201810912782.4A CN201810912782A CN108977389A CN 108977389 A CN108977389 A CN 108977389A CN 201810912782 A CN201810912782 A CN 201810912782A CN 108977389 A CN108977389 A CN 108977389A
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- CN
- China
- Prior art keywords
- spirulina
- wheel animalcule
- electric shock
- culturing
- killing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A kind of method that killing wheel animalcule in process of culturing spirulina belongs to spirulina breeding technical field, and this method use the spirulina liquid in the direct current electric shock spirulina culture pond that DC voltage is 24V, the wheel animalcule in killing spirulina liquid；Concrete operation method: the electric shock carries out once every other day, need to carry out 3-6 electric shock, and electric shock is the both ends that yin-yang the two poles of the earth of direct current are inserted into spirulina liquid lie farthest away respectively every time, is shocked by electricity 15 minutes；The electric-shocking method of wheel animalcule, has no toxic side effect in killing process of culturing spirulina provided by the invention, and good disinsection effect will not remain in culturing liquid, to prevent pollution of food chain；The method that electric shock of the invention kills wheel animalcule can make spirulina breeding production capacity promote 30% or more, improve traditional cultural method while rapidly and efficiently administering wheel animalcule, reduce the series of problems such as the high cost of tradition cultivation.
Description
Technical field
The invention belongs to spirulina breeding technical fields, specifically a kind of that wheel animalcule is killed in process of culturing spirulina
Method.
Background technique
Spirulina is a kind of low water plant, is grown under environment with high salt and high-alkali, has been had 3,500,000,000 years away from the present
History；The pH value for being suitble to it to grow is between 9.5-10.5.Viable microorganism is seldom in such a case, and wheel animalcule is it
One；In cultivating spirulina, wheel animalcule can cause destructive injury to spiral frond.
Traditional killing wheel animalcule is all addition ammonium bicarbonate, and ammonia is allowed to kill wheel animalcule, but when killing wheel animalcule
The usage amount of ammonium bicarbonate hold it is bad and can to spiral frond generate poisoning and cause certain injury, and this method can only at
Worm plays a role, be to wheel animalcule spore it is inoperative, wheel animalcule spore can hatch into adult again over time.Wheel animalcule
Fertility is again extremely strong, if repeatedly with ammonium bicarbonate kill wheel animalcule will generate variation to spiral frond and to influence one whole
The yield and quality in year.So maximum pest and disease damage is exactly wheel animalcule for cultivating spirulina.In the culturing liquid of spirulina originally
Body just has a biological chain (could also say that food chain), and wheel animalcule is the top for belonging to food chain.
Country's spirulina breeding enterprise governance wheel animalcule effective ways are few at present, in actual production, insect pest in order to prevent,
Chemosterilant is usually utilized to administer algae insect pest, but since cultivation dealer lacks healthy aquaculture consciousness, blindly large dosage is anti-
It is used repeatedly using pesticide and chemosterilant or even many illegal drugs again, causes algae medicament residue phenomenon occur, cause
There are serious security risks for aquatic products.
Summary of the invention
It is an object of the invention to overcome shortcoming in the prior art, one kind is provided and is killed in process of culturing spirulina
It goes out the method for wheel animalcule.
In order to achieve the object of the present invention, we will adopt the following technical scheme that and be implemented.
A method of killing wheel animalcule in process of culturing spirulina, it is characterised in that: this method is shocked by electricity using direct current
Spirulina liquid in spirulina culture pond kills the wheel animalcule in spirulina liquid.
Further, the DC voltage is 24V.
Further, the concrete operation method of the method: the electric shock carries out primary every other day, need to carry out 3~6 times, every time
Electric shock is the both ends of lie farthest away in the spirulina liquid that yin-yang the two poles of the earth of direct current are respectively connected in spirulina culture pond, electric shock
5~25 minutes.
Further, the optimal electric shock time is 15 minutes.
Further, the interval number of days every other day is preferably 1~3 day.
Further, the optimal interval number of days is 1 day.
Further, the optimal number of shocks is 4 times.
Beneficial effect
The motor method of wheel animalcule, has no toxic side effect in killing process of culturing spirulina provided by the invention, good disinsection effect,
It will not remain in culturing liquid, to prevent pollution of food chain.The method that electric shock of the invention kills wheel animalcule, rapidly and efficiently
While administering wheel animalcule, spirulina breeding production capacity can be made to promote 30% or more, improve traditional cultural method, reduce tradition
The series of problems such as the high cost of cultivation.
Specific embodiment
In conjunction with the embodiments, the present invention is described further.
In one embodiment, a method of in process of culturing spirulina kill wheel animalcule, use DC voltage for
Spirulina liquid in the direct current electric shock spirulina culture pond of 24V, kills the wheel animalcule in spirulina liquid.
In one embodiment, the concrete operation method of the method: the electric shock carried out once, to carry out 4 times every 1 day
Electric shock, electric shock is the both ends that yin-yang the two poles of the earth of direct current are inserted into spirulina liquid lie farthest away respectively every time, is shocked by electricity 15 minutes.
Its principle is can be by most spore and almost all of imago killing in normal electric shock；Even if there is residual
And the spore that do not kill, it shocks by electricity and is killed again when can also hatch during its hatching or just into larva.
In the above-described embodiments, number of shocks and electric shock interval number of days determination: mainly those skilled in the art with
By means of the experience of cultivating spirulina, finds out and take turns the incubation cycle of worm's ovum in process of culturing spirulina to determine, incubation cycle
Determination be to be determined according to the temperature of spirulina liquid existing for wheel worm's ovum, according to the incubation cycle of wheel worm's ovum, setting electric shock
The case where number and electric shock interval time, the present invention is according to the spirulina cultivated, using above-mentioned concrete operation method: every 1
It is once shocked by electricity, 8 days carry out 4 times electric shock, will maximumlly by spirulina liquid wheel animalcule and its spore thoroughly kill
It goes out clean.
Claims (7)
1. a kind of method for killing wheel animalcule in process of culturing spirulina, it is characterised in that: this method is using direct current electric shock spiral shell
The spirulina liquid in algae culture pond is revolved, the wheel animalcule in spirulina liquid is killed.
2. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 1, it is characterised in that: described
DC voltage is 24V.
3. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 1, it is characterised in that: described
The concrete operation method of method: the electric shock carries out once every other day, need to carry out 3~6 times, and electric shock is by the yin-yang of direct current every time
The two poles of the earth are respectively connected to the both ends of lie farthest away in the spirulina liquid in spirulina culture pond, shock by electricity ten~25 minutes.
4. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 3, it is characterised in that: described
Interval number of days every other day is preferably 1~3 day.
5. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 4, it is characterised in that: described
Being spaced number of days optimal interval number of days is 1 day.
6. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 3, it is characterised in that: described
The optimal number of number of shocks is 4 times.
7. a kind of method for killing wheel animalcule in process of culturing spirulina according to claim 3, it is characterised in that: described
Electric shock time optimal selects 15 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810912782.4A CN108977389A (en) | 2018-08-03 | 2018-08-03 | A method of killing wheel animalcule in process of culturing spirulina |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810912782.4A CN108977389A (en) | 2018-08-03 | 2018-08-03 | A method of killing wheel animalcule in process of culturing spirulina |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108977389A true CN108977389A (en) | 2018-12-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810912782.4A Pending CN108977389A (en) | 2018-08-03 | 2018-08-03 | A method of killing wheel animalcule in process of culturing spirulina |
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| CN (1) | CN108977389A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534438A (en) * | 2020-04-16 | 2020-08-14 | 华南农业大学 | Method for efficiently killing protozoa in living unicellular algae and application |
Citations (4)
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| JP2006230328A (en) * | 2005-02-28 | 2006-09-07 | Hokkaido Univ | Method for sterilizing rotifera monogenital egg |
| WO2010038912A2 (en) * | 2008-10-03 | 2010-04-08 | Sekine Toshirou | Microalgae culture method and device |
| CN103109810A (en) * | 2011-11-16 | 2013-05-22 | 中国科学院海洋研究所 | Rotifer killing medicine, and preparation method and application thereof |
| CN107439426A (en) * | 2017-08-23 | 2017-12-08 | 何亮 | A kind of cultural method of loach |
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2018
- 2018-08-03 CN CN201810912782.4A patent/CN108977389A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006230328A (en) * | 2005-02-28 | 2006-09-07 | Hokkaido Univ | Method for sterilizing rotifera monogenital egg |
| WO2010038912A2 (en) * | 2008-10-03 | 2010-04-08 | Sekine Toshirou | Microalgae culture method and device |
| CN103109810A (en) * | 2011-11-16 | 2013-05-22 | 中国科学院海洋研究所 | Rotifer killing medicine, and preparation method and application thereof |
| CN107439426A (en) * | 2017-08-23 | 2017-12-08 | 何亮 | A kind of cultural method of loach |
Non-Patent Citations (1)
| Title |
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| D. REGO等: "Control of predators in industrial scale microalgae cultures with Pulsed Electric Fields", 《BIOELECTROCHEMISTRY》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534438A (en) * | 2020-04-16 | 2020-08-14 | 华南农业大学 | Method for efficiently killing protozoa in living unicellular algae and application |
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