CN108938508B

CN108938508B - Fig fruit extract with sun-screening and synergistic effects and application thereof

Info

Publication number: CN108938508B
Application number: CN201811196187.1A
Authority: CN
Inventors: 李丽; 董银卯; 孟宏; 黄晓羽
Original assignee: Beijing Technology and Business University
Current assignee: Beijing Technology and Business University
Priority date: 2018-10-15
Filing date: 2018-10-15
Publication date: 2021-03-30
Anticipated expiration: 2038-10-15
Also published as: CN108938508A

Abstract

The invention relates to a fig fruit extract and application thereof. The fig fruit extract is used as a natural plant raw material and added into a sunscreen product, has broad-spectrum sunscreen synergistic capability, and can properly reduce the dosage of a physical and chemical sunscreen agent. The sunscreen product has good stability and safety, good concealer and brightening effects, good skin feel and smell, reduced irritation risk, and wide market application prospect.

Description

Fig fruit extract with sun-screening and synergistic effects and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a fig fruit extract with sun-screening and synergistic effects and application thereof.

Background

Because the current commercial sunscreen products are dominated by physical and chemical sunscreens, the using amount of the sunscreen products has a plurality of influences on the aspects of skin feel, safety and the like, and the natural plant raw materials are applied to the sunscreen products in a great trend along with the pursuit of people on green safety concepts. Plant-derived sunscreen ingredients mainly comprise alkaloids, fatty acids, steroids, polyphenols, polysaccharides and polypeptides, and when used alone, the sunscreen factor (SPF) is low, so that an ideal sunscreen effect cannot be achieved.

Fig used as a medicine and food homologous fig is praised as the human health guard of the 21 st century because of being rich in various nutritional elements such as fiber, polyphenol, vitamin, organic acid and the like. The current research situation mainly aims at fig leaves, the emphasis is on the medical fields of evaluation of antibiosis, anticancer, cytotoxicity and the like, and the emphasis is on adding the perfume type flavoring agent into products in the aspect of cosmetics.

The effect Of fig fruit Extract-Containing emulsions In the formulation on human Skin Parameters, including Skin melanin, erythema, epidermal hydration and sebum secretion, was studied In Khan, H., Akhtar, N., & Ali, A. (2014.) Effects Of extracts on human Skin Parameters, including Skin melanin, erythema, epidermal hydration and sebum secretion. The basic formula containing 4% fig fruit extract was applied to the cheek skin of random volunteers for 8 weeks as a test reagent and as a control. Test results show that the water-in-oil emulsion containing fig fruit extract in the formula can effectively reduce the skin melanin content, reduce the epidermis moisture loss, reduce the grease secretion and enhance the skin hydration. It is therefore readily apparent that some of the fig extracts are used in the prior art of cosmetics, and their moisturizing and whitening effects have only been studied; there is no concern about the application of fig fruit extract in cosmetics and its synergistic effect on sunscreen agents, i.e., its use in sunscreen products.

The patent application No. 201711499544.7 discloses a novel sunscreen synergistic composition. The composition comprises radix Arnebiae extract, rhizoma Zingiberis recens extract, bisabolol and gallocatechin gallate, has no sunscreen effect, but can be added into conventional sunscreen agent to greatly improve sunscreen index. The invention mainly screens the components with synergistic effect in the composition and the optimal addition amount of the corresponding components, discusses the sunscreen synergistic effect of the composition in different substrates, but does not research the synergistic effect of the fig fruit extract on different sunscreen formulations.

Therefore, in view of the above situation, the fig fruit extract is applied to sunscreen products, and the synergistic effect of the fig fruit extract on different sunscreen formulations is explored, so that the fig fruit extract has very important significance for reducing the addition amount of sunscreen agents in cosmetic formulations, reducing the irritation of cosmetics to skin and achieving the concept of green, safe and sunscreen.

The invention is provided under the technical background, is obviously different from the prior art, and the invention uses the fig and fig fruit extract for preparing the sun-screening product, and unexpectedly finds that the invention has obvious synergistic effect on different types of sun-screening agents.

Disclosure of Invention

Aiming at the market demand and the defects of the prior art, the invention aims to develop the sunscreen synergistic effect of fig and fig fruit extracts by adding the fig and fig fruit extracts into physical, chemical and physicochemical compound sunscreen formulas in a gradient manner.

The invention provides application of figs in preparation of a sun-screening product.

Furthermore, the invention implements the application of different parts of the fig in the preparation of sunscreen products.

Hereinafter, fig fruits are used as a representative example to describe the technical solution of the present invention, but it does not mean that other parts of fig cannot achieve the same or similar effects achieved by fig fruits. That is, extracts from other parts of the fig plant can achieve the same or similar effects as the fig fruit extracts described below.

As a particular, exemplary set of embodiments, the present invention provides the use of a fig fruit extract in the preparation of a sunscreen product.

The invention adopts the following steps in the implementation process, including but not limited to: fig fruit extract extracted according to the extraction method disclosed by publicly published papers, patents and other publicly known legal public approaches; fig fruit extract obtained by a legal gifting route; ficus carica fruit extract obtained by a legal commercial purchase route.

The above various approaches to obtaining fig fruit extract can achieve the same or similar technical effects in this group of embodiments.

The group of embodiments is illustrated below by way of example of commercially available fig fruit extracts.

The fig fruit extract obtained by the commercial purchase way is purchased from Shaanxi Xian health raw material workshop, and the product goods number is F6008, and the batch number is 201803001A.

In the implementation process of the invention, the commercially purchased fig fruit extract is measured, and comprises fig fruit total flavone, fig fruit total polysaccharide and fig fruit total polyphenol, wherein the contents of the fig fruit total flavone, the fig fruit total polysaccharide and the fig fruit total polyphenol are 0.0064mg/mL, 1.46mg/mL and 0.0047mg/mL respectively.

The fig fruit extract with the content reaches a certain technical effect aiming at a certain technical problem in the group of embodiments; the fig fruit extract with other component contents can also obtain corresponding technical effects in other technical problems.

In the practice of this group of embodiments, fig fruit extract is used in combination with a sunscreen agent to prepare the sunscreen product described above.

That is, the sunscreen product includes a sunscreen agent and fig fruit extract.

The sunscreen agent is a physical sunscreen agent, a chemical sunscreen agent or a physical and chemical compound sunscreen agent.

The physical sunscreen agents include, but are not limited to, titanium dioxide.

The chemical sunscreen agent includes but is not limited to ethylhexyl methoxycinnamate.

The physical and chemical compound sunscreen agent is a mixture of a physical sunscreen agent and a chemical sunscreen agent;

the physical and chemical compound sunscreen agent comprises but is not limited to ethylhexyl methoxycinnamate and titanium dioxide.

In some embodiments, the physical sunscreen agent comprises titanium dioxide.

In other specific embodiments, the chemical sunscreen agent comprises ethylhexyl methoxycinnamate.

In still other embodiments, the physicochemical combination sunscreen agent is ethylhexyl methoxycinnamate with titanium dioxide.

In some more specific embodiments, the physical sunscreen agent is titanium dioxide, which is present in the sunscreen product in an amount of 4% to 25% by weight, and the fig fruit extract is present in the sunscreen product in an amount of 0.01% to 1.13% by weight.

In other more specific embodiments, the chemical sunscreen agent is ethylhexyl methoxycinnamate in an amount from 4% to 10% by weight of the sunscreen product, and the fig fruit extract is from 0.01% to 1.11% by weight of the sunscreen product.

In still more specific embodiments, the physicochemical compounded sunscreen agent is ethylhexyl methoxycinnamate and titanium dioxide, the titanium dioxide accounts for 5-25% by weight of the sunscreen product, the ethylhexyl methoxycinnamate accounts for 5-10% by weight of the sunscreen product, and the fig fruit extract accounts for 0.01-1.07% by weight of the sunscreen product.

The sunscreen product may also include any additive conventionally used in the art that may be used therein.

Compared with the prior art, the invention has the following beneficial effects:

(1) in the implementation process, the fig fruit extract and the sun-screening agent are mixed and used in the sun-screening product, and the weight percentage of the fig fruit extract and the sun-screening agent is controlled, so that the sun-screening effect of the sun-screening product can be effectively improved.

(2) The fig fruit extract used as a natural plant raw material for a sunscreen product has potential capacities of antibiosis, antioxidation, anti-aging and whitening, namely has visual effects of concealing and no need of adding essence, can properly reduce the dosage of physical and chemical sunscreens, reduces the risk of cosmetic irritation caused by the increase of the dosage of the sunscreens to a certain extent, and has wide market application prospect.

Detailed Description

The fig fruit extract (hereinafter referred to as fruit extract a) used herein is purchased from shanxi west' an healthy raw material house (the extract is obtained by extracting 1 kg of product from 10 kg of raw material, additives such as fig preservative are soluble in water, and fig fruit source is fig of moraceae plant). The apparatus and reagents used in the present invention are shown in Table 1.

TABLE 1 test instruments and reagents

1. Extract solubility test

1.1 lipid solubility test

1.00g of the fruit extract A was weighed in 10mL of anhydrous ethanol, and stirred uniformly, a large amount of precipitate appeared, and the mixture was layered upon slight standing.

1.2 Water solubility test

Weighing 1.00g of fruit extract A in 10mL of deionized water, adding the fruit extract A in a gradient manner per 10mL of deionized water, heating and stirring at 70 ℃ for 10min, performing ultrasonic treatment for 5min (ultrasonic treatment is 28 ℃ and 70% power), and when the deionized water is added to 60mL, a clear, transparent and special-smell brown yellow uniform solution is presented, and a small amount of small-particle substances are arranged at the bottom of a standing beaker. When the fruit extract A is added to 6g of the glycerin compound solution according to the gradient of every 1g, the small particles at the cup bottom are almost completely dissolved.

In summary, in the present invention, 1.00g of fruit extract A can be completely dissolved in 60mL of deionized water and 6g of glycerol.

2. Preparation of sunscreen products

To exclude interference that the fig fruit extract itself may have sunscreen capacity, the highest added concentration of fruit extract a was added to the formulation without any sunscreen ingredients, set as a blank group. Table 2 is the blank component.

TABLE 2 blank group Components

Sunscreen products were prepared by mixing fig fruit extracts of different content with different sunscreen ingredients, respectively, to obtain the following 3 sets of examples.

Example set 1

TABLE 3 Effect on titanium dioxide

Example group 2

TABLE 4 Effect of ethylhexyl p-methoxycinnamate

EXAMPLE group 3

TABLE 5 Effect on physicochemical sunscreen combinations (ethylhexyl methoxycinnamate + titanium dioxide)

Comparative example group 1

TABLE 6 Effect on benzophenone-3

Comparative example 2

Manxileidun new aqua sun protection lotion (SPF ═ 25).

2.1 preparation Process

The above blanks, examples 1-3 and comparative example 1 were prepared as follows:

(1) respectively weighing phase A and phase B according to formula and dosage, and respectively heating to 85 deg.C;

(2) pouring the phase A into the phase B, homogenizing at 5600r/min for 5min, adding polysorbate-60, homogenizing for 1min, cooling to 40 deg.C, adding phenoxyethanol and 1,2 hexanediol, stirring at 300r/min, cooling to 28 deg.C, and packaging into 10mL centrifuge tubes to obtain sunscreen product.

2.2SPF value test

Currently, methods for testing the SPF index of the sun-screening product at home and abroad include a human body testing method and an in-vitro instrument testing method, which are respectively restricted by different factors. However, the human body test method has the defects of long time consumption, troublesome operation, high cost, large individual difference, difficult selection of tested groups and the like. In contrast, the in vitro instrument test protocol is a little superior. The clinical trial proves the relevance of the SPF index in-vitro instrument test method and the human body test method of the sunscreen cosmetic. The results show that the two have good correlation (r is 0.9616), and the SPF values measured by the two methods have no significant difference (0.40 < P < 0.50). Therefore, the test uses an in vitro instrument test method to evaluate the SPF index of the previous sample, the sun protection index analyzer can automatically calculate the SPF value, Boots Star, MPF value and absorbance, the UVA/UVB test value can be completely displayed, and test reports and graphs can be printed. The test environment is a constant temperature and humidity chamber, the temperature is controlled at (20 +/-2) DEG C, and the relative humidity is controlled at (50 +/-5)%.

2.2.1 coated Carrier investigation

Before the actual measurement, 3 sets of parallel tests were carried out on one sample using medical tape and a Polymethylmethacrylate (PMMA) plate, respectively, to examine the stability of the two carriers according to the measurement procedure. The results show that the values measured using PMMA plates are less deviating than with the tape method, so that the support used in the following tests is a PMMA plate.

2.2.2 test procedure

Coating a sample: each of the samples of examples 1 to 3 and comparative example 1 was sampled at 2mg/cm using a dedicated spotter²And (3) putting on a PMMA plate, wearing medical finger sleeves to uniformly smear, wherein no visible sample exists on the finger sleeves before and after smearing, all samples are smeared by adopting the same method, and each sample is subjected to 3 groups of parallel tests. And (5) placing the smeared sample plate in a dark environment to naturally form a film for 20min to be detected.

Correcting the instrument: before testing, the calibration filter disc carried by the instrument is used for scanning 6 points at the edge and the center of the PMMA hollow plate under a 125W continuous xenon lamp light source to calibrate, so that the accuracy of the test is ensured.

Test samples: each sample was tested using an SPF-290s Sun protection index Analyzer in the same manner as the calibrated instrument. The instrument draws 6 light wavelengths MPF (Monochromatic Protection Factor) for each sample, and related indexes of the sample are calculated by instrument matching software.

To minimize the measurement error, the samples obtained from the formulation gradient test were placed in a dark environment at room temperature for 48h to ensure stability of the paste or solution, to obtain table 7.

TABLE 7 measured SPF values

Note: the SPF value for the blank was found to be 0.67 by assay.

Group of examples A0, B0 and C0 are contrasted with the other examples in the corresponding group of examples. From the blank set and the measured SPF values in table 7, the following conclusions can be drawn:

(1) the fig fruit extract has no sun-screening effect;

(2) the sunscreen synergistic objects of the fig fruit extract are selective, and the synergistic effect is optimal when the weight percentage of the fig fruit extract and the sunscreen agent in a sunscreen product is within a certain range, the fig fruit extract has a sunscreen synergistic linear result on titanium dioxide or ethylhexyl methoxycinnamate, and a sunscreen synergistic linear result on benzophenone-3;

(3) the fig fruit extract has a linear synergistic effect on a linearly synergistic sunscreen combination (ethylhexyl methoxycinnamate + titanium dioxide), and the synergistic effect is optimal when the fig fruit extract and the sunscreen account for a certain weight percentage in the sunscreen product.

3 stability evaluation of sunscreen products

The present inventors examined the stability of the sunscreen products of example groups 1-3, comparative example group 1 and comparative example 2. The samples obtained in the examples 1 to 3, the comparative example 1 and the comparative example 2 were placed in 7 conditions of room temperature, room temperature and dark environment, light, heat environment (45 + -1 deg.C opaque air-drying box), refrigerated 4 deg.C, frozen environment (-15 + -1 deg.C), alternate cooling and heating (-15 + -1 deg.C and 45 + -1 deg.C 24 h), and the characteristic changes were observed after a certain period of time, and the results of the examination are shown in tables 8 and 9.

TABLE 8 sunscreen product 28 day stability observations

TABLE 9 Observation of the stability of sunscreen products over 78 days

Note: "-" indicates no change from the initial state; "1" means there is a little floc; "2" indicates a slightly reduced sweetness in a special flavor; "3" means that the bottom is slightly darker in color than the upper layer; wherein 1 and 3 are shaken up and then are restored to the initial state.

The stability observation result of the fig sunscreen product shows that the fig extract is used in the sunscreen product, and the sunscreen product has good stability and no change of floccules, smell, color and the like. Also, comparative example 2 is also superior in stability. The sunscreen product of comparative example 1 was relatively poor in stability, and had problems of little floc, slightly reduced sweetness in a peculiar smell, slightly darker bottom color than the upper layer, and the like in both 28 days and 78 days.

4 evaluation of safety of Fig fruit extract

The safety of the fig fruit extract is detected by an erythrocyte hemolytic blood coagulation test.

Red blood cell hemolysis (RBC) is one of the alternatives to Draize test, which evaluates the damage of chemicals to eye tissue cells by measuring the amount of hemoglobin exudation and the degree of deformation, and the RBC test is mainly used for the eye irritation research of chemicals such as cosmetic products and raw materials internationally.

The reagent used in the invention is fig fruit extract compound solution, and the method is a standard erythrocyte hemolysis blood coagulation test method. As is clear from Table 10, the extract solution was non-irritating and highly safe.

TABLE 10 Fig fruit extract Compound solution Red blood cell hemolysis test (RBC) results

5 evaluation of efficacy of sunscreen product

5.1 test reagents

The products of groups A1, B4, C3, A0, B0 and C0 in examples 1-3, and D1 in comparative example 1 and comparative example 2 were randomly selected.

5.2 test methods

The 18 volunteers meeting the requirements are found for trial, blind test (unnamed) is adopted, the product sample is smeared on the skin (such as the inner side of an arm, the back of a hand and the like) of a human body, and corresponding evaluation is given to the overall feeling of the product, the specific subjective feelings such as absorption effect, spreading property, moistening effect, fineness, glossiness, viscosity, concealing property, freshness and the like. The evaluation of each item was carried out with a scoring system: preferably 9 to 10 minutes; preferably 7 to 8 minutes; generally 5 to 6 points; worse 3-4 points; very poor 1-2 points.

5.3 test data

The human trials were combined for populations of different skin types, whose sensory usage evaluations are shown in table 11:

TABLE 11 sensory evaluation of products

Note: sensory evaluation data in the table are average values of evaluation scores of 18 volunteers.

5.4 conclusion of the test

Tests show that after the fig fruit extract is added, the fineness and the concealer score of the cosmetic are over 9 points, and the satisfaction degree is good; the coating and refreshing degree are both 7-8 minutes, so that the paint is satisfactory; the absorption effect, the moistening effect, the glossiness and the viscosity are all 8-10 minutes, the overall feeling of the selected people on the target product is good, and the sun-screening product is superior to the sun-screening product without the fig fruit extract. The manxifulon new aquamarine sunscreen lotion has relatively high SPF value and good stability, but the product efficacy is relatively weaker than that of the lotion.

In conclusion, the sunscreen product disclosed by the invention meets the marketing requirement of cosmetics through stability and safety tests, has a good concealing and brightening effect, is good in skin feel and smell, and has a wide application prospect.

The above embodiments are only for illustrating the technical solutions of the present invention, and are not to be construed as limiting the scope of the present invention, and those skilled in the art can make simple modifications or equivalent substitutions on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. Synergistic use of fig fruit extract for sunscreen agents in the preparation of sunscreen products;

the sunscreen product comprises a sunscreen agent, wherein the sunscreen agent is a physical sunscreen agent, a chemical sunscreen agent or a physical and chemical compound sunscreen agent;

the physical sun-screening agent is titanium dioxide; the chemical sun-screening agent is ethylhexyl methoxycinnamate; the physical and chemical compound sunscreen agent is a mixture of titanium dioxide and ethylhexyl methoxycinnamate;

the fig fruit extract contains fig fruit total flavonoids, fig fruit total polysaccharides and fig fruit total polyphenols.

2. Use according to claim 1, characterized in that: wherein the fig fruit extract accounts for 0.01-1.13% of the sunscreen product.

3. Use according to claim 2, characterized in that: wherein the fig fruit extract accounts for 0.01-1.11% of the sunscreen product.