CN108934805B - High-quality eucommia ulmoides planting method - Google Patents

High-quality eucommia ulmoides planting method Download PDF

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Publication number
CN108934805B
CN108934805B CN201810366946.8A CN201810366946A CN108934805B CN 108934805 B CN108934805 B CN 108934805B CN 201810366946 A CN201810366946 A CN 201810366946A CN 108934805 B CN108934805 B CN 108934805B
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eucommia ulmoides
grape seed
seed liquid
days
treatment
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CN108934805A (en
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王宗正
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Anhui Zongzheng Agriculture Technology Development Co ltd
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Anhui Zongzheng Agriculture Technology Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a high-quality eucommia ulmoides planting method, which relates to the technical field of traditional Chinese medicine planting, wherein 8-age eucommia ulmoides is subjected to drought stress treatment, the water content of the eucommia ulmoides is kept sufficient in 1 month, the drought treatment is started for 30-40 days in the beginning of 2 months, then grape seed liquid is adopted to spray the surfaces of the eucommia ulmoides leaves for 5 times continuously, the spraying of the grape seed liquid is stopped at intervals of 2 days every time, the eucommia ulmoides is subjected to normal watering, the drought treatment is started for 35-45 days in the beginning of 9 months, then the grape seed liquid is adopted to spray the surfaces of the eucommia ulmoides leaves for 5 times continuously, the spraying of the grape seed liquid is stopped at intervals of 2 days every time, the second treatment period is adopted, the eucommia ulmoides is subjected to normal watering, and eucommia ulmoides barks are taken when the age is full 10, namely; the invention obviously improves the content of the flavone and the gutta-percha in the eucommia ulmoides and improves the quality of the eucommia ulmoides.

Description

High-quality eucommia ulmoides planting method
Technical Field
The invention belongs to the technical field of traditional Chinese medicine planting, and particularly relates to a high-quality eucommia ulmoides planting method.
Background
Eucommia bark is deciduous tree of family eucommia of order Eucommiaceae of class Hamamelidae of class Magnoliaceae, also known as Chinese rippled bark, floss silk bark, Sixian, Sec, kapok, Sixian, and Silianpi, and is a famous and precious medicinal plant in China, with the continuous and intensive research on eucommia bark in various fields of medical treatment, industry, health care and the like, especially gutta-percha with gum-plastic duality has attracted people's extensive attention, besides the gutta-percha, the eucommia bark also contains chlorogenic acid, flavonoid compounds and other effective components with the effects of resisting bacteria, benefiting gallbladder, stopping bleeding, increasing leukocytes and resisting viruses, and the quercetin in the flavonoid compounds has the effect of resisting free radicals and the like.
However, the eucommia ulmoides planted in the prior art has relatively low content of eucommia ulmoides gum and eucommia ulmoides flavonoids, and cannot meet the market demand.
Disclosure of Invention
The invention aims to provide a high-quality eucommia ulmoides planting method aiming at the existing problems.
The invention is realized by the following technical scheme:
a high-quality eucommia ulmoides planting method comprises the steps of carrying out drought stress treatment on eucommia ulmoides at 8 ages, keeping water quantity sufficient in eucommia ulmoides in 1 month, carrying out drought treatment for 30-40 days at the beginning of 2 months, then adopting grape seed liquid to spray the surfaces of eucommia ulmoides leaves for 5 times continuously, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a first treatment period, then normally watering the eucommia ulmoides, carrying out drought treatment for 35-45 days at the beginning of 9 months, then adopting the grape seed liquid to spray the surfaces of the eucommia ulmoides leaves for 5 times continuously, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a second treatment period, then normally watering the eucommia ulmoides, and taking eucommia ulmoides bark when the eucommia ulmoides is full of 10 ages.
Further, the grape seed liquid is prepared from the following components in parts by weight: 15-20 parts of grape seeds, 0.15-0.19 part of mellitic acid, 1.3-1.5 parts of brassinosteroid and 100 parts of water.
Further, the preparation method of the grape seed liquid comprises the following steps:
(1) drying the grapes until the water content is 15%, then crushing, and sieving with a 120-mesh sieve to obtain grape seed powder;
(2) carrying out reflux extraction on the grape seed powder twice by taking a 50% ethanol solution as a solvent, wherein the volume ratio of the solvent to the grape seed powder is 300 mL: 80g, extracting for 3.5 hours, then combining the two extracting solutions, then filtering to obtain filtrate, treating the filtrate by microwave for 10-15s, and then freeze-drying to obtain solid extract;
(3) dissolving the obtained solid in water, adding benzene hexacarboxylic acid and brassinosteroids, and stirring uniformly to obtain the grape seed liquid.
Further, the microwave power is 350W.
Furthermore, the drought treatment is not carried out by human water supply, and meanwhile, the rain water is prevented from falling onto the eucommia ulmoides by human intervention during rainfall.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the first treatment period is 320-350mL for each plant.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the second treatment period is 380mL for each plant.
Further, the normal watering is performed once every 3-5 days, and the amount of water for each watering is 500 mL.
Compared with the prior art, the invention has the following advantages: the production of eucommia ulmoides flavonoids is a complex biological process, relates to a plurality of metabolic pathways, and needs the participation of a plurality of enzymes and hormones, the eucommia ulmoides is treated in a drought stress treatment mode, so that the eucommia ulmoides generates a plurality of stress regulation reactions under the drought stress, then even the grape seed liquid prepared by the method is sprayed on the surface of the eucommia ulmoides subjected to the drought stress, the grape seed liquid contains a plurality of active ingredients, the signal transmission process in the eucommia ulmoides under the stress can be effectively promoted, the transpiration effect of the eucommia ulmoides plants can be reduced, the synthesis and accumulation of flavonoids and gutta percha in the eucommia ulmoides plants can be promoted, meanwhile, the physiological reaction under the stress of the eucommia ulmoides can be rapidly relieved, the content of the flavonoids and the gutta percha in the eucommia ulmoides is obviously improved, and the quality of the eucommia ulmoides is.
Detailed Description
Example 1
A high-quality eucommia ulmoides planting method includes the steps of conducting drought stress treatment on eucommia ulmoides at 8 ages, keeping water quantity sufficient in eucommia ulmoides in 1 month, conducting drought treatment for 30 days at the beginning of 2 months, then adopting grape seed liquid to spray the surfaces of eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a first treatment period, then conducting normal watering on the eucommia ulmoides, conducting drought treatment for 35 days at the beginning of 9 months, then adopting the grape seed liquid to spray the surfaces of the eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a second treatment period, then conducting normal watering on the eucommia ulmoides, and starting taking eucommia ulmoides barks when the eucommia ulmoides is full of 10 ages.
Further, the grape seed liquid is prepared from the following components in parts by weight: 15 parts of grape seeds, 0.15 part of mellitic acid, 1.3 parts of brassinosteroids and 100 parts of water.
Further, the preparation method of the grape seed liquid comprises the following steps:
(1) drying the grapes until the water content is 15%, then crushing, and sieving with a 120-mesh sieve to obtain grape seed powder;
(2) carrying out reflux extraction on the grape seed powder twice by taking a 50% ethanol solution as a solvent, wherein the volume ratio of the solvent to the grape seed powder is 300 mL: 80g, extracting for 3.5 hours, then combining the two extracting solutions, then filtering to obtain filtrate, treating the filtrate by microwave for 10s, and then freeze-drying to obtain solid extract;
(3) dissolving the obtained solid in water, adding benzene hexacarboxylic acid and brassinosteroids, and stirring uniformly to obtain the grape seed liquid.
Further, the microwave power is 350W.
Furthermore, the drought treatment is not carried out by human water supply, and meanwhile, the rain water is prevented from falling onto the eucommia ulmoides by human intervention during rainfall.
Furthermore, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the first treatment period is 320mL per plant.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the second treatment period is 360mL for each plant.
Further, the normal watering is once every 3 days, and the amount of water for each time is 500 mL.
Example 2
A high-quality eucommia ulmoides planting method includes the steps of conducting drought stress treatment on eucommia ulmoides at 8 ages, keeping water quantity sufficient in eucommia ulmoides in 1 month, conducting drought treatment for 40 days at the beginning of 2 months, then adopting grape seed liquid to spray the surfaces of eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a first treatment period, then conducting normal watering on the eucommia ulmoides, conducting drought treatment for 45 days at the beginning of 9 months, then adopting the grape seed liquid to spray the surfaces of the eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a second treatment period, then conducting normal watering on the eucommia ulmoides, and starting taking eucommia ulmoides barks when the eucommia ulmoides is full of 10 ages.
Further, the grape seed liquid is prepared from the following components in parts by weight: grape seed 20, mellitic acid 0.19, brassinosteroid 1.5 and water 100.
Further, the preparation method of the grape seed liquid comprises the following steps:
(1) drying the grapes until the water content is 15%, then crushing, and sieving with a 120-mesh sieve to obtain grape seed powder;
(2) carrying out reflux extraction on the grape seed powder twice by taking a 50% ethanol solution as a solvent, wherein the volume ratio of the solvent to the grape seed powder is 300 mL: 80g, extracting for 3.5 hours, then combining the two extracting solutions, then filtering to obtain filtrate, treating the filtrate by microwave for 15s, and then freeze-drying to obtain solid extract;
(3) dissolving the obtained solid in water, adding benzene hexacarboxylic acid and brassinosteroids, and stirring uniformly to obtain the grape seed liquid.
Further, the microwave power is 350W.
Furthermore, the drought treatment is not carried out by human water supply, and meanwhile, the rain water is prevented from falling onto the eucommia ulmoides by human intervention during rainfall.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the first treatment period is 350mL per plant.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the second treatment period is 380mL for each plant.
Further, the normal watering is performed once every 3-5 days, and the amount of water for each watering is 500 mL.
Example 3
A high-quality eucommia ulmoides planting method includes the steps of conducting drought stress treatment on eucommia ulmoides at 8 ages, keeping water quantity sufficient for the eucommia ulmoides in 1 month, conducting drought treatment for 35 days at the beginning of 2 months, then adopting grape seed liquid to spray the surfaces of eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a first treatment period, then conducting normal watering on the eucommia ulmoides, conducting drought treatment for 40 days at the beginning of 9 months, then adopting the grape seed liquid to spray the surfaces of the eucommia ulmoides leaves, continuously spraying for 5 times, wherein 2 days are spaced each time, then stopping spraying the grape seed liquid to serve as a second treatment period, then conducting normal watering on the eucommia ulmoides, and starting taking eucommia ulmoides barks when the eucommia ulmoides is full of 10 ages.
Further, the grape seed liquid is prepared from the following components in parts by weight: grape seed 18, mellitic acid 0.16, brassinosteroid 1.4 and water 100.
Further, the preparation method of the grape seed liquid comprises the following steps:
(1) drying the grapes until the water content is 15%, then crushing, and sieving with a 120-mesh sieve to obtain grape seed powder;
(2) carrying out reflux extraction on the grape seed powder twice by taking a 50% ethanol solution as a solvent, wherein the volume ratio of the solvent to the grape seed powder is 300 mL: 80g, extracting for 3.5 hours, then combining the two extracting solutions, then filtering to obtain filtrate, treating the filtrate by microwave for 12s, and then freeze-drying to obtain solid extract;
(3) dissolving the obtained solid in water, adding benzene hexacarboxylic acid and brassinosteroids, and stirring uniformly to obtain the grape seed liquid.
Further, the microwave power is 350W.
Furthermore, the drought treatment is not carried out by human water supply, and meanwhile, the rain water is prevented from falling onto the eucommia ulmoides by human intervention during rainfall.
Further, the spraying amount of the grape seed liquid spraying the surface of the eucommia ulmoides leaves in the first treatment period is 330mL per plant.
Further, the spraying amount of the grape seed liquid for spraying the surface of the eucommia ulmoides leaves in the second treatment period is 370mL for each plant.
Further, the normal watering is performed once every 4 days, and the amount of water for each watering is 500 mL.
Comparative example 1: the differences from example 1 are only that the spraying time and the spraying amount of the grape seed liquid are not changed without drought stress treatment.
Comparative example 2: only the difference from embodiment 1 is that only the first treatment cycle treatment is performed.
Comparative example 3: only the difference from embodiment 1 is that only the second treatment cycle treatment is performed.
Comparative example 4: the only difference from example 1 is that the grape seed liquid was replaced with clear water.
Comparative example 5: the only difference from example 1 is that mellitic acid is not added to the grape seed liquid.
Comparative example 6: the difference from example 1 is that grape seed solution is not added with grape seed solid extract.
The bark of eucommia bark of the same position is taken from eucommia bark of the same age of 10 years after the eucommia bark of the same growth vigor is treated by the method of the embodiment and the comparative example, the content of gutta percha and the content of eucommia flavone in each group of 3g of eucommia bark are detected, and the comparison is carried out:
extracting the eucommia ulmoides gum for 2 hours by using petroleum ether; extracting eucommia ulmoides flavonoids for 2 hours by using an ethanol solution with the volume fraction of 70 percent;
TABLE 1
Figure DEST_PATH_IMAGE002
As can be seen from the above Table 1, the treatment method of the present invention can effectively increase the content of gutta-percha and the content of eucommia ulmoides flavonoids in eucommia ulmoides.

Claims (8)

1. A high-quality eucommia ulmoides planting method is characterized in that 8-age eucommia ulmoides is subjected to drought stress treatment, water quantity of the eucommia ulmoides is kept sufficient in 1 month, drought treatment is carried out for 30-40 days at the beginning of 2 months, then grape seed liquid is sprayed on the surfaces of eucommia ulmoides leaves for 5 times continuously, the spraying of the grape seed liquid is stopped at intervals of 2 days each time, the first treatment period is used, the eucommia ulmoides is watered normally, then drought treatment is carried out for 35-45 days at the beginning of 9 months, then the grape seed liquid is sprayed on the surfaces of the eucommia ulmoides leaves for 5 times continuously, the spraying of the grape seed liquid is stopped at intervals of 2 days each time, the second treatment period is used, the eucommia ulmoides is watered normally, and eucommia ulmoides bark is taken when the eucommia ulmoides is full of 10 ages.
2. The method for planting high-quality eucommia ulmoides as claimed in claim 1, wherein the grape seed liquid is prepared from the following components in parts by weight: 15-20 parts of grape seeds, 0.15-0.19 part of mellitic acid, 1.3-1.5 parts of brassinosteroid and 100 parts of water.
3. The method for planting high-quality eucommia ulmoides as claimed in claim 2, wherein the preparation method of the grape seed liquid comprises the following steps:
(1) drying the grapes until the water content is 15%, then crushing, and sieving with a 120-mesh sieve to obtain grape seed powder;
(2) carrying out reflux extraction on the grape seed powder twice by taking a 50% ethanol solution as a solvent, wherein the volume ratio of the solvent to the grape seed powder is 300 mL: 80g, extracting for 3.5 hours, then combining the two extracting solutions, then filtering to obtain filtrate, treating the filtrate by microwave for 10-15s, and then freeze-drying to obtain solid extract;
(3) dissolving the obtained solid in water, adding benzene hexacarboxylic acid and brassinosteroids, and stirring uniformly to obtain the grape seed liquid.
4. The method for planting high-quality eucommia ulmoides as claimed in claim 3, wherein the microwave power is 350W.
5. The method for planting high-quality eucommia ulmoides as claimed in claim 1, wherein the drought treatment is not artificial water supply, and human intervention is performed during rainfall to prevent rainwater from falling on the eucommia ulmoides.
6. The method as claimed in claim 1, wherein the amount of the grape seed solution sprayed on the surface of the eucommia ulmoides leaves in the first treatment period is 320-350mL per plant.
7. The method as claimed in claim 1, wherein the amount of the grape seed solution sprayed on the surface of the eucommia ulmoides leaves in the second treatment period is 380mL for each plant, 360-.
8. The method for planting high-quality eucommia ulmoides as claimed in claim 1, wherein the normal watering is performed once every 3-5 days, and the amount of water per watering is 500 mL.
CN201810366946.8A 2018-04-23 2018-04-23 High-quality eucommia ulmoides planting method Expired - Fee Related CN108934805B (en)

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AR067433A1 (en) * 2007-04-18 2009-10-14 Performance Plants Inc A PLANT THAT HAS TOLERANCE INCREASED TO THERMAL VOLTAGE.
CN102517144A (en) * 2011-11-28 2012-06-27 宁夏大学 Method for extracting oil and procyanidin from grape seeds
CN105230453A (en) * 2015-08-31 2016-01-13 南京江宁台湾农民创业园发展有限公司 Planting method for dendrobium officinale
CN106465632A (en) * 2016-09-06 2017-03-01 合肥元政农林生态科技有限公司 A kind of implantation methods of high yield Radix hemerocalis plicatae
CN106961972A (en) * 2017-03-30 2017-07-21 上海孙桥现代农业联合发展有限公司 A kind of implantation methods for improving Celery content

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