CN108934783A - A kind of oyster mushroom culture medium and preparation method thereof - Google Patents

A kind of oyster mushroom culture medium and preparation method thereof Download PDF

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Publication number
CN108934783A
CN108934783A CN201811157888.4A CN201811157888A CN108934783A CN 108934783 A CN108934783 A CN 108934783A CN 201811157888 A CN201811157888 A CN 201811157888A CN 108934783 A CN108934783 A CN 108934783A
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Prior art keywords
culture medium
parts
oyster mushroom
mushroom culture
preparation
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CN201811157888.4A
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Chinese (zh)
Inventor
周红
张晓燕
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Anhui Copper Grass Flower Modern Agriculture Technology Co Ltd
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Anhui Copper Grass Flower Modern Agriculture Technology Co Ltd
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Priority to CN201811157888.4A priority Critical patent/CN108934783A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of oyster mushroom culture medium, raw material includes: reed root, corn stover, corncob, ramulus cinnamomi, straw, perilla leaf, lotus leaf, vinasse, ginger powder, corn flour, coix seed powder, urea, calcium superphosphate, gypsum, lime.The invention also discloses a kind of preparation methods of oyster mushroom culture medium.The present invention is full of nutrition, and Growth of Pleurotus Mycelium and sporophore growth can be promoted to develop, and the oyster mushroom biological efficiency cultivated is high, good in economic efficiency.

Description

A kind of oyster mushroom culture medium and preparation method thereof
Technical field
The present invention relates to agricultural product technical fields, and in particular to a kind of oyster mushroom culture medium and preparation method thereof.
Background technique
Edible mushroom is to constitute the good essential biology of the ecosystem, and China is Edible Fungi and consumption big country, mesh First more than 50 kinds of cultivation type, certain status is occupied in the national economic development, it has also become in agricultural after planting industry, aquaculture Another pillar industry, but traditional compost is mostly used weed tree sawdust, cotton seed hulls, and it is raw that these materials much can not meet edible mushroom The needs of production.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of oyster mushroom culture medium and preparation method thereof, battalion It supports and enriches, Growth of Pleurotus Mycelium and sporophore growth can be promoted to develop, improve the yield and quality of oyster mushroom, biological efficiency It is high.
The invention proposes a kind of oyster mushroom culture medium, raw material includes: 20-30 parts of reed roots, 60-70 parts of corns by weight Stalk, 15-30 part corncob, 6-10 parts of ramulus cinnamomi, 20-35 parts of straw, 10-15 parts of perilla leafs, 10-20 parts of lotus leaves, 15-30 portions of wine Grain, 2-6 parts of ginger powders, 5-10 portions of corn flour, 2-8 parts of coix seed powders, 2-6 parts of urea, 1-2 parts of calcium superphosphate, 1-3 parts of gypsum, 2-4 Part lime.
The invention also provides a kind of preparation methods of oyster mushroom culture medium, include the following steps:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into The particle of 3-6mm size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water that 3-9 times is measured, obtain spice Liquid;
S2, straw, perilla leaf, lotus leaf are crushed, mixed-powder A, hybrid particles B is added, when spice liquid is added in sieving Stirring, is subsequently added into lime and stirs evenly, and stands, and vinasse, ginger powder, corn flour, coix seed powder are then added, and is uniformly mixed, Add clear water, obtains mixture;
S3, mixture is built up to trapezoidal heap, jack is ventilative, and fermentation material can be obtained in fermentation, turning;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, atmospheric steam sterilizing, Then oyster mushroom culture medium can be obtained in natural cooling.
Preferably, in S1, corn stover, corncob are respectively crushed into the particle of 3-6mm size.
Preferably, in S1, urea, calcium superphosphate, gypsum are dissolved in the water that 3-9 times is measured.
Preferably, in S2, the mesh number of the sieving is 20-40 mesh.
Preferably, in S2, the time of the standing is 1-2h.
Preferably, in S2, adding clear water to water content is 60-65%.
Preferably, in S3, mixture is built up into trapezoidal heap, jack is ventilative, heat preservation when mixture temperature rises to 60-65 DEG C Ferment 18-22h, turning, and the heat-preservation fermentation 18-22h, then turning again when mixture temperature rises to 60-65 DEG C again repeats 3- 4 times, fermentation material can be obtained.
Preferably, in S3, the bottom of the trapezoidal heap is 1.2-1.5m, upper low for 0.8-1m, a height of 0.6-1.0m.
Preferably, in S4, to fermentation material cooled to room temperature, it is packed into bacterium bag, compacting, and fasten bacterium bag both ends, be placed in 100 DEG C of atmospheric steams sterilizing 10-14h, then naturally cool to 20-24 DEG C, oyster mushroom culture medium can be obtained.
The present invention makes full use of reed root, corn stover, corncob, ramulus cinnamomi, straw, perilla leaf, lotus leaf, vinasse, ginger powder, jade The raw materials such as rice flour, coix seed powder, it is full of nutrition, it can satisfy the nutritional requirement of Growth of Pleurotus Mycelium and sporophore growth development, And raw material sources are extensive, cheap, at low cost, high conversion rate, also reduced caused by burning to a certain degree to atmosphere, soil The pollution of earth, water quality, environment, lengthening manufacturing chain increase industry added value;Wherein, the present invention is added to reed root, perilla leaf, osmanthus Branch, ginger powder are used in conjunction with, and reed root is cold in nature, and perilla leaf, ramulus cinnamomi, ginger powder are warm-natured, and compatible use, which can produce, enhances its antibacterial and growth-promoting Long effect reduces or avoids culture medium to the inhibitory effect of mycelia to enhance the effect of the anti-pathogenic bacteria of culture medium, improve to jade The utilization rate of the raw materials such as rice stalk, corncob, corn flour, promotes the growth and development of oyster mushroom, in addition, also adding egg in culture medium The content of the nutriments such as white matter, vitamin B1, B2, C, asparagine, polysaccharide, furfural and water-soluble saccharides, further Promote growth, improves the quality and yield of oyster mushroom.
Specific embodiment
In the following, technical solution of the present invention is described in detail by specific embodiment.
Embodiment 1
A kind of oyster mushroom culture medium, raw material include: 30 parts of reed roots, 60 parts of corn stovers, 30 parts of corncobs, 6 by weight Part ramulus cinnamomi, 35 parts of straw, 10 parts of perilla leafs, 20 parts of lotus leaves, 15 parts of vinasse, 6 parts of ginger powders, 5 portions of corn flour, 8 parts of coix seed powders, 2 Part urea, 2 parts of calcium superphosphate, 1 part of gypsum, 4 parts of lime.
The preparation method of the oyster mushroom culture medium, includes the following steps:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into The particle of 3mm size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water of 9 times of amounts, obtain spice liquid;
S2, straw, perilla leaf, lotus leaf are crushed, crosses 20 meshes, mixed-powder A, hybrid particles B is added, spice is added in side The stirring of liquid side, is subsequently added into lime and stirs evenly, and stands 2h, and vinasse, ginger powder, corn flour, coix seed powder, mixing is then added Uniformly, adding clear water to water content is 60%, obtains mixture;
S3, mixture is built up to trapezoidal heap, lower bottom width 1.5m, upper low width 0.8m, high 1.0m, jack is ventilative, mixture warm Degree heat-preservation fermentation 22h when rising to 60 DEG C, turning, the heat-preservation fermentation 22h again when mixture temperature rises to 60 DEG C again, then turn over Heap is repeated 3 times, and fermentation material can be obtained;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, be placed in 100 DEG C of normal pressures Then steam sterilizing 14h naturally cools to 20 DEG C, oyster mushroom culture medium can be obtained.
Embodiment 2
A kind of oyster mushroom culture medium, raw material include: 20 parts of reed roots, 70 parts of corn stovers, 15 parts of corncobs, 10 by weight Part ramulus cinnamomi, 20 parts of straw, 15 parts of perilla leafs, 10 parts of lotus leaves, 30 parts of vinasse, 2 parts of ginger powders, 10 portions of corn flour, 2 parts of coix seed powders, 6 Part urea, 1 part of calcium superphosphate, 3 parts of gypsum, 2 parts of lime.
The preparation method of the oyster mushroom culture medium, includes the following steps:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into The particle of 6mm size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water of 3 times of amounts, obtain spice liquid;
S2, straw, perilla leaf, lotus leaf are crushed, crosses 40 meshes, mixed-powder A, hybrid particles B is added, spice is added in side The stirring of liquid side, is subsequently added into lime and stirs evenly, and stands 1h, and vinasse, ginger powder, corn flour, coix seed powder, mixing is then added Uniformly, adding clear water to water content is 65%, obtains mixture;
S3, mixture is built up to trapezoidal heap, lower bottom width 1.2m, upper low width 1m, high 0.6m, jack is ventilative, mixture temperature Heat-preservation fermentation 18h when rising to 65 DEG C, turning, the heat-preservation fermentation 18h again when mixture temperature rises to 65 DEG C again, then turn over Heap is repeated 4 times, and fermentation material can be obtained;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, be placed in 100 DEG C of normal pressures Then steam sterilizing 10h naturally cools to 24 DEG C, oyster mushroom culture medium can be obtained.
Embodiment 3
A kind of oyster mushroom culture medium, raw material include: 26 parts of reed roots, 62 parts of corn stovers, 25 parts of corncobs, 7 by weight Part ramulus cinnamomi, 30 parts of straw, 11 parts of perilla leafs, 18 parts of lotus leaves, 20 parts of vinasse, 5 parts of ginger powders, 6 portions of corn flour, 7 parts of coix seed powders, 3 Part urea, 2 parts of calcium superphosphate, 2 parts of gypsum, 3 parts of lime.
The preparation method of the oyster mushroom culture medium, includes the following steps:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into The particle of 4mm size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water of 8 times of amounts, obtain spice liquid;
S2, straw, perilla leaf, lotus leaf are crushed, crosses 30 meshes, mixed-powder A, hybrid particles B is added, spice is added in side The stirring of liquid side, is subsequently added into lime and stirs evenly, and stands 1.5h, and vinasse, ginger powder, corn flour, coix seed powder are then added, and mixes It closes uniformly, adding clear water to water content is 64%, obtains mixture;
S3, mixture is built up to trapezoidal heap, lower bottom width 1.3m, upper low width 0.9m, high 0.7m, jack is ventilative, mixture warm Degree heat-preservation fermentation 19h when rising to 64 DEG C, turning, the heat-preservation fermentation 19h again when mixture temperature rises to 63 DEG C again, then turn over Heap is repeated 4 times, and fermentation material can be obtained;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, be placed in 100 DEG C of normal pressures Then steam sterilizing 11h naturally cools to 23 DEG C, oyster mushroom culture medium can be obtained.
Embodiment 4
A kind of oyster mushroom culture medium, raw material include: 24 parts of reed roots, 67 parts of corn stovers, 20 parts of corncobs, 9 by weight Part ramulus cinnamomi, 25 parts of straw, 14 parts of perilla leafs, 14 parts of lotus leaves, 25 parts of vinasse, 3 parts of ginger powders, 8 portions of corn flour, 4 parts of coix seed powders, 5 Part urea, 1 part of calcium superphosphate, 2 parts of gypsum, 3 parts of lime.
The preparation method of the oyster mushroom culture medium, includes the following steps:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into The particle of 5mm size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water of 5 times of amounts, obtain spice liquid;
S2, straw, perilla leaf, lotus leaf are crushed, crosses 30 meshes, mixed-powder A, hybrid particles B is added, spice is added in side The stirring of liquid side, is subsequently added into lime and stirs evenly, and stands 1.5h, and vinasse, ginger powder, corn flour, coix seed powder are then added, and mixes It closes uniformly, adding clear water to water content is 62%, obtains mixture;
S3, mixture is built up to trapezoidal heap, lower bottom width 1.4m, upper low width 0.9m, high 0.9m, jack is ventilative, mixture warm Degree heat-preservation fermentation 20h when rising to 62 DEG C, turning, the heat-preservation fermentation 21h again when mixture temperature rises to 62 DEG C again, then turn over Heap is repeated 3 times, and fermentation material can be obtained;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, be placed in 100 DEG C of normal pressures Then steam sterilizing 12h naturally cools to 22 DEG C, oyster mushroom culture medium can be obtained.
Comparative example 1
Compared with Example 4, reed root, perilla leaf, ramulus cinnamomi, ginger powder are not added, and other conditions are identical with embodiment 4.
Test example 1
1-4 of the embodiment of the present invention is respectively adopted and the culture medium therein of comparative example 1 carries out mushroom cultivation, observes the life of oyster mushroom Long situation is simultaneously recorded, and the results are shown in Table 1.
Table 1
Project Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
Mycelial growth rate (cm/d) 1.22 1.15 1.12 1.26 0.83
Purseful number of days (d) 24 25 25 24 29
Fruiting time (d) 30 31 31 30 36
Biological efficiency (%) 133.6 131.2 129.8 133.5 89.5
Bacterium bag pollution rate (%) 0.6 0.7 0.5 0.6 1.9
As can be seen from Table 1, culture medium of the present invention is full of nutrition, and Growth of Pleurotus Mycelium and sporophore growth can be promoted to send out It educates, mycelial growth rate is fast, and culture medium utilization rate is high and pollution rate is low, and the Yield of Pleurotus Ostreatus of institute's output is high, high-quality, economic benefit It is good.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (10)

1. a kind of oyster mushroom culture medium, which is characterized in that its raw material includes: 20-30 parts of reed roots, 60-70 parts of corn stalks by weight Stalk, 15-30 part corncob, 6-10 parts of ramulus cinnamomi, 20-35 parts of straw, 10-15 parts of perilla leafs, 10-20 parts of lotus leaves, 15-30 portions of wine Grain, 2-6 parts of ginger powders, 5-10 portions of corn flour, 2-8 parts of coix seed powders, 2-6 parts of urea, 1-2 parts of calcium superphosphate, 1-3 parts of gypsum, 2-4 Part lime.
2. a kind of preparation method of oyster mushroom culture medium according to claim 1, which comprises the steps of:
S1, reed root, ramulus cinnamomi are pulverized respectively, is mixed to get mixed-powder A;Corn stover, corncob are respectively crushed into 3-6mm The particle of size mixes to obtain hybrid particles B;Urea, calcium superphosphate, gypsum are dissolved in the water that 3-9 times is measured, obtain spice liquid;
S2, straw, perilla leaf, lotus leaf are crushed, sieving is added mixed-powder A, hybrid particles B, stirs when spice liquid is added It mixes, be subsequently added into lime and stir evenly, stand, vinasse, ginger powder, corn flour, coix seed powder are then added, be uniformly mixed, add Clear water obtains mixture;
S3, mixture is built up to trapezoidal heap, jack is ventilative, and fermentation material can be obtained in fermentation, turning;
S4, to fermentation material cooled to room temperature, be packed into bacterium bag, compacting, and fasten bacterium bag both ends, atmospheric steam sterilizing, then Oyster mushroom culture medium can be obtained in natural cooling.
3. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S1, by corn stover, corncob It is respectively crushed into the particle of 3-6mm size.
4. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S1, by urea, calcium superphosphate, Gypsum is dissolved in the water that 3-9 times is measured.
5. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S2, the mesh number of the sieving is 20-40 mesh.
6. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S2, the time of the standing is 1-2h。
7. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S2, add clear water to the water content to be 60-65%.
8. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S3, mixture is built up trapezoidal Heap, jack is ventilative, heat-preservation fermentation 18-22h when mixture temperature rises to 60-65 DEG C, turning, when mixture temperature rises again Heat-preservation fermentation 18-22h, then turning again when to 60-65 DEG C, repeats 3-4 times, fermentation material can be obtained.
9. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S3, the bottom of the trapezoidal heap It is upper low for 0.8-1m, a height of 0.6-1.0m for 1.2-1.5m.
10. the preparation method of oyster mushroom culture medium according to claim 1, which is characterized in that in S4, to fermentation material natural cooling To room temperature, it is packed into bacterium bag, compacting, and fastens bacterium bag both ends, is placed in 100 DEG C of atmospheric steam sterilizing 10-14h, then natural cooling To 20-24 DEG C, oyster mushroom culture medium can be obtained.
CN201811157888.4A 2018-09-30 2018-09-30 A kind of oyster mushroom culture medium and preparation method thereof Pending CN108934783A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115404175A (en) * 2022-10-12 2022-11-29 北京雷力海洋生物新产业股份有限公司 Fermentation medium of trichoderma viride, trichoderma agent and preparation method of trichoderma agent

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CN103340099A (en) * 2013-07-24 2013-10-09 四川省砚山中药饮片有限公司 Method for planting oyster mushrooms by using corncob with straw
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CN104472216A (en) * 2014-12-04 2015-04-01 黎平县黎丰菌业专业合作社 Pleurotus ostreatus cultivation method
CN104557318A (en) * 2015-01-25 2015-04-29 邬金飞 Preparation method of mushroom cultivation material
CN105432321A (en) * 2015-12-14 2016-03-30 唐山市农业科学研究院 Oyster mushroom culture medium processing method
CN106278467A (en) * 2016-08-16 2017-01-04 郑州市蔬菜研究所 A kind of mushroom cultivation material and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102173886A (en) * 2011-01-20 2011-09-07 青岛农业大学 Oyster mushroom culture medium as well as fermentation inoculum and application thereof
CN103371053A (en) * 2012-04-29 2013-10-30 何寒 Method for cultivating green natural oyster mushrooms by using pure-length rice straws
CN103340099A (en) * 2013-07-24 2013-10-09 四川省砚山中药饮片有限公司 Method for planting oyster mushrooms by using corncob with straw
CN103483074A (en) * 2013-09-13 2014-01-01 遵义师范学院 Edible mushroom culture material and production method thereof
CN104472216A (en) * 2014-12-04 2015-04-01 黎平县黎丰菌业专业合作社 Pleurotus ostreatus cultivation method
CN104557318A (en) * 2015-01-25 2015-04-29 邬金飞 Preparation method of mushroom cultivation material
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CN106278467A (en) * 2016-08-16 2017-01-04 郑州市蔬菜研究所 A kind of mushroom cultivation material and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115404175A (en) * 2022-10-12 2022-11-29 北京雷力海洋生物新产业股份有限公司 Fermentation medium of trichoderma viride, trichoderma agent and preparation method of trichoderma agent

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