CN108929164A - A kind of bio-bacterial manure and preparation method thereof preventing and treating eggplant root rot - Google Patents
A kind of bio-bacterial manure and preparation method thereof preventing and treating eggplant root rot Download PDFInfo
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- CN108929164A CN108929164A CN201810907536.XA CN201810907536A CN108929164A CN 108929164 A CN108929164 A CN 108929164A CN 201810907536 A CN201810907536 A CN 201810907536A CN 108929164 A CN108929164 A CN 108929164A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/007—Fertilisers made from animal corpses, or parts thereof from derived products of animal origin or their wastes, e.g. leather, dairy products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The present invention provides a kind of bio-bacterial manure and preparation method thereof for preventing and treating eggplant root rot, belong to technical field of fertilizers, the raw material comprising following parts by weight: 50~150g of luffa, 2~20g of bent essence, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, 5~15g of non-pathogenic fusarium bacterium.The present invention passes through the microbial synergies such as carrier luffa and song essence, ferment, symbiosis is formed with eggplant, bent essence and ferment decompose the field planting of the nutriment and microorganism that generate, improve eggplant Nutrient Absorption efficiency, then the ecological niche of fusarium solanae is occupied using non-pathogenic fusarium bacterium, effectively inhibit the proliferation of pathogen, bio-bacterial manure of the invention can be used for improving eggplant quality and increase its yield.
Description
Technical field
The present invention relates to technical field of fertilizers, in particular to a kind of bio-bacterial manure for preventing and treating eggplant root rot and its preparation side
Method.
Background technique
Eggplant is the important industrial crops in China, to be that the main vegetables of daily life congratulate flavouring.It is with fusarium solanae
Main soil-borne disease is an important factor for restricting eggplant production, and in order to improve eggplant yield and prevention and treatment pest and disease damage, farmer is being planted
A large amount of chemical fertilizer and pesticide are put into during planting, with a large amount of applications of chemical fertilizer, pesticide, is guaranteeing and increasing the same of plant growth
When, a series of adverse effects are brought to soil environment and crop, subtract soil shortage organic matter, hardened, acidification, adhesion strength
Weak, nutrient loss, crop cannot be metabolized chemical fertilizer element etc. completely.In order to reduce chemical fertilizer and applications of pesticide amount, life is had developed
Object bacterial manure, specific microorganism, vegetable-derived components are added to novel, efficient, the safe microorganism-formed in organic fertilizer to be had
Machine object fertilizer is rich in a large amount of benefit materials, comprising: a variety of organic acids, peptides and abundant including nitrogen, phosphorus, potassium
Nutrient can not only provide nutrition for crops, and fertilizer efficiency is long, can increase and update the soil organism, promote microorganism
Breeding, improves the physicochemical property and bioactivity of soil, but existing eggplant is not high with microbial manure fertilizer efficiency, and water-retaining property
It can be poor.
China Patent No. CN201710337309.3 discloses a kind of production of anti-eggplant bacterial wilt complex micro organism fungicide
Technique, including colloid bacillus cereus, trichoderma harzianum, streptomyces microflavus, chitosan oligosaccharide, plant greasy filth plant ash；Utilize chitosan oligosaccharide
The disease resistance of plant is induced, plant stress-resistance ability is enhanced, still, chitosan oligosaccharide has broad-spectrum antibacterial activity, not only inhibits to eggplant
Harmful microorganism also will appear certain killing effect to beneficial microbe, so that structure of soil microbial community be made to lack of proper care；
Secondly, preventing and treating bacterial wilt, certain journey using biological control combination bacterial strain-colloid bacillus cereus, trichoderma harzianum, streptomyces microflavus
Degree reduces the influence to structure of soil microbial community, but bacterial strain uses therefor has a single function, and lacks microorganism to organic matter
Decomposition causes fertilizer efficiency to reduce, and reduces eggplant to the absorption efficiency of nutriment；Furthermore micro- life in the actual production process
The loss of object is bigger, not easy to maintain, and effective bacterial content is too low, keeps its getting fat resistant effect limited.
Therefore, exploitation can either increase soil fertility, and improve soil, preserve the ecological environment, and improve crop yield and quality,
The bio-bacterial manure of the soil-borne disease of energy antagonism crop again, for promoting agricultural benign cycle and sustainable development with important meaning
Justice.
Summary of the invention
The purpose of the present invention is to provide a kind of bio-bacterial manures and preparation method thereof for preventing and treating eggplant root rot, pass through carrier
The microbial synergies such as luffa and song essence, ferment form symbiosis with eggplant, and bent essence and ferment decompose the nutriment generated
And the field planting of microorganism, eggplant Nutrient Absorption efficiency is improved, the life of fusarium solanae is then occupied using non-pathogenic fusarium bacterium
State position, effectively inhibits the proliferation of pathogen, and bio-bacterial manure of the invention can be used for improving eggplant quality and increase its yield.
Above-mentioned technical purpose of the invention has the technical scheme that
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: 50~150g of luffa, bent essence
2~20g, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-pathogenic fusarium bacterium 5
~15g.
Further, the luffa is magnetic luffa.
Further, the activated biomass is humus, yolk, at least two in six ring stones.
Further, the N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea.
Further, plant ash, calcium superphosphate, urea adding proportion (by mass) be 5~7:1:4.
A kind of preparation method for the bio-bacterial manure preventing and treating eggplant root rot, comprising the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, bent smart fermentation liquid, shaken cultivation is added in luffa；
S3, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Further, the luffa is magnetic luffa, the preparation method comprises the following steps: deionized water is added in the reactor
100g、Fe3O4 0.2g、MgSO4 0.15g、MnSO4 0.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse at suspension,
Luffa is added, mixed solution is ultrasonically treated, then filters, is dry, obtaining magnetic luffa.
Further, the condition of the step S2 shaken cultivation are as follows: 25~37 DEG C, 40~100rpm, shaken cultivation 48~
72h。
Further, the condition of the step S3 compost fermentation are as follows: first aerobic fermentation 2d, 20~30d of subsequent decomposed fermentation,
Period, every 2d turning was primary.
A kind of application for the bio-bacterial manure preventing and treating eggplant root rot, the bio-bacterial manure is for inhibiting eggplant root rot.
The beneficial effects of the present invention are:
1. using luffa as carrier, luffa has biggish specific surface area, flourishing pore structure and degradable right
It is environmental-friendly, it is added in organic fertilizer, water retention in soil and the confinement capabilities to nutrient, increase organic carbon can be improved
Accumulation and influence microbial population development and stable state and enhance improve soil microbe quantity and activity etc., Jin Ergai
Kind soil aggregate structure, oxygen content, aeration status, fertility etc., promote plant growth, improve yield.
2. be fixed to luffa surface by the microorganism that luffa adsorbs ferment and Qu Jingzhong, one improves biology
Amount, microorganism is stable and not easily runs off, and resistance to murder by poisoning ability is strong, and two carry out surface aggregation microorganism degrades under aerobic/anoxia condition
Organic matter in soil increases the content of available N and P, potassium, sulphur etc. in soil, increase ASI systematic approach, while inside
Microbial degradation loofah fiber is bred for the microbe survival on surface, and the activity of being kept fixed microorganism is unattenuated, until
Luffa degradation is finished, the microorganism Spontaneous release of absorption increases diversity of soil microorganism into soil.
3. first co-culturing Qu Jingyu luffa, ferment compost is added, to make microorganism and the luffa of Qu Jingzhong
It comes into full contact with, is adsorbed in the porous structure of luffa, the high active enzyme of secretion makes the cellulose dissolution of luffa and secretes one
A little biotic factors etc., provide nutrient for subsequent ferment Microorganism colonization, can also make the cell membrane swelling of plant duff
Softening destroys, and makes to contain the protein in cell and carbohydrate release, decomposes for ferment, then improve nutrition in soil
The utilization rate of element, and promote raw material quick composting, shorten the compost time；Secondly, by the degradation of enzyme to luffa into
Row is modified, and makes its rough surface, and very Big mutation rate occurs for hole, small to one nanometer, greatly to tens nanometers or even some tens of pm, shape
The microenvironment that life can be inhabited at microorganism is conducive to the attachment of subsequent ferment microorganism and non-pathogenic fusarium bacterium；Another party
Fiber unformed partial hydrolysis in face makes the hole of crystal region become larger, and helps to improve water-retaining property and water absorption.
4. the proliferation of pathogen can be effectively suppressed using non-pathogenic fusarium bacterium as biocontrol microorganisms, with fusarium solanae exist compared with
High niche overlap, therefore fusarium solanae available resources can be made to reduce, reached by preferentially seizing the resource in rhizosphere soil
To the purpose for inhibiting fusarium solanae, and there is no the nutrient competition with other microorganisms, the ecologic structure of indigenous flora is not influenced.
5. forming symbiosis by the way that the microorganisms such as bent essence, ferment and eggplant are added, bent essence and ferment decompose the battalion generated
The field planting for supporting substance and microorganism, have stimulated the root growth of eggplant and increases root absorption area, improve the nutrient of eggplant
Then absorption efficiency occupies the ecological niche of fusarium solanae using non-pathogenic fusarium bacterium, effectively inhibit the proliferation of pathogen, promotes
The growth and development of eggplant can be used for improving eggplant quality in bio-bacterial manure agriculturally of the invention and increase its yield, has
Huge economic benefit.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with specific embodiment.Obviously, described
Embodiment be only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiment of the present invention, ability
Domain those of ordinary skill every other embodiment obtained without creative efforts, belongs to guarantor of the present invention
The range of shield.
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: 50~150g of luffa, bent essence
2~20g, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-pathogenic fusarium bacterium 5
~15g.
Luffa is the natural vascular bundle that mature sponge gourd fruit removes the cellulosic reticular structure obtained after crust and seed
Tissue, is made of 60% cellulose, 30% hemicellulose and 10% lignin, has on morphosis continuous hollow
Microcellular structure, formed vascular bundle simultaneously generate multichannel and multistage pore system, this unique space structure makes its large specific surface area,
Specific surface area average value is up to 123m2/ g, and adsorption capacity is strong, it is 3 times or so of material self weight that water absorption is big, mean water absorption
Rate 2106g/m2, average resolution factor 98.27%, no pollution to the environment.The present invention using luffa as microbe carrier have with
Lower benefit:
First, luffa rough surface has porous structure, provides habitat and space for the existence of microorganism, be
The growth and breeding of peculiar microorganism provides breeding ground, increases Soil Biodiversity, while regulating and controlling the physics and chemistry of Soil Micro-environment
Property stores moisture and nutrient, influences and regulate and control the growth and development and metabolism of edaphon, and then improve soil fertility；Together
The fiber of Shi Zuowei microbe carrier, luffa is relatively high to the compatibility of microorganism, is conducive to microorganism attachment, biological load
Amount is high, and cellulose is degraded during soil reaction, will be slow release carbon source, supplies microorganism growth and development and metabolism,
Conducive to microbial activities, soil microbe quantity and activity can be improved.
Second, after the vesicular texture of luffa, suitable porosity make it apply farmland, be conducive to increase soil total
Porosity, capillary porosity and aeration porosity can quickly absorb moisture to improve the wicking of soil, and be maintained at soil
In earth hole, the effective moisture content and moisture holding capacity of soil are improved, and luffa is rich in cellulose, hemicellulose and lignin etc.
Hydroaropic substance is also beneficial to save moisture.
Third, luffa delay bio-bacterial manure release in the soil and leaching loss as carrier sticking part nutrient；Sponge gourd
Network generates absorption to mineral cation by its surface acidity functional group and acts on withholding, reduces the dissolution of water-soluble nutrient ions
Migration, avoids the leaching loss of nutrient, and continues in the soil and slowly discharge, and the sustained release for being equivalent to nutrient carries
Body improves utilization rate of fertilizer to achieve the effect that keep fertility；Secondly, luffa is similar to sponge state, in organic fertilizer fermentation
In the process, microcosmic effect can be carried out with other material compositions, the loose and porous structure having can improve the knot of organic fertilizer
Structure makes organic fertilizer after shaping, and sponge state institutional framework is also presented can effectively change after entering applying fertilizer in soil
The hardened state of kind soil, forms loose porous soil texture, improves oxygen content, oxidation-reduction quality and the aeration status of soil
Deng to increase the yield of soil fertility and crops.
4th, soil granular is soil organic colloid such as humus and inorganic colloid such as calcium carbonate, calcium sulfate etc. by more
The electrostatic interaction of valence cation, which bonds, to be formed, after luffa adsorbs mineral cation, be agron, activated biomass,
Calcium superphosphate etc. provides bridging substance-bivalent cation abundant, and the electrostatic attraction for making it through positive and negative charge quickly agglomerates,
Soil organic-inorganic glue compound is formed, soil granular is promoted to be formed, the formation of soil granular can keep soil from packing together, and save
Moisture, the consumption and accumulation for coordinating nutrient reduce nutrition loss, provide tilth to a soil.
In short, luffa has biggish specific surface area, flourishing pore structure and degradable environmentally friendly, addition
Into organic fertilizer, water retention in soil can be improved and to the confinement capabilities of nutrient, the accumulation of increase organic carbon and shadow
The development of sound microbial population and stable state and the activity etc. for enhancing edaphon, and then improve soil texture, oxygen content, lead to
Vaporous condition, fertility etc. promote plant growth.But to promote the increase of crop yield, it is to be unable to reach that luffa, which is administered alone,
, need the synergistic effect of mineral nutrient element and microorganism such as ferment.
Ferment, also known as ferment fertilizer are a kind of microbial manures, by nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, saccharomycete, unwrapping wire
What bacterium, fungi formed can generate the profitable strains of a variety of catalytic decomposition enzymes, these biological floras energy in culture reproductive process
Ten several inhomogeneous enzymes such as amylase, protease, lipase, lactalase are generated, catalytic decomposition weeds, plant duff etc. have
Machine substance, and the chemical substances such as chemical fertilizer, pesticide can be decomposed, the minerals such as shale, zeolite, bentonite can also be decomposed, are allowed to
The effective component utilized for plant is converted into short time, release stores a large amount of fixed phosphorus potassium and micro member in the soil
Element increases soil nutrient availability, while microorganism breeds at an amazing speed in the soil, and the secretion of generation can promote soil
The formation of earth crumb structure, eliminates hardened, and then increases soil permeability, water-retaining property, builds up fertility with sustainability etc., improving
Crop growth environment, the effect for reducing fertilizer and pesticide amount.
Bent essence, using prolease activity and the higher aspergillus oryzae of saccharifying enzymic activity and aspergillus niger by dry, separation and Extraction
Effective spore, sealed package form, and spore count is up to 2 × 1010A/g or more is usually used in fermentation and prepares soy sauce, improvement face
The flavor of sauce, beans sauce；Bent essence based on aspergillus oryzae and aspergillus niger, the pectases of two kinds of bacterial strains, hemicellulase, cellulase,
Protease, the enzyme activity of carbohydrase are all higher, and pectase, cellulase etc. can make the cellulose dissolution of luffa, are subsequent
Ferment Microorganism colonization provides nutrition, and the cell membrane swelling of plant duff can also be made to soften and destroyed, make to contain in cell
Protein and carbohydrate release, for ferment decompose, then improve Nutrient Elements in Soil utilization rate, secondly, to silk
Melon network is modified.
The proliferation of pathogen can be effectively suppressed as biocontrol microorganisms for non-pathogenic fusarium bacterium, higher with fusarium solanae presence
Niche overlap reduce fusarium solanae available resources, reach suppression therefore by preferentially seizing resource in rhizosphere soil
The purpose of fusarium solanae processed.Under the conditions of optimum temperature and nutrient concentration, inhibition energy of the non-pathogenic fusarium bacterium to fusarium solanae
Power is strong.
The present invention forms symbiosis by the way that the microorganisms such as bent essence, ferment and eggplant are added, and bent essence and ferment, which decompose, to be generated
Nutriment and microorganism field planting, have stimulated the root growth of eggplant and increase root absorption area, improve eggplant
Nutrient Absorption efficiency, resist eggplant root system it is unsound phenomena such as, then occupy fusarium solanae using non-pathogenic fusarium bacterium
Ecological niche effectively inhibits the proliferation of pathogen, promotes eggplant growth and development, can use in bio-bacterial manure agriculturally of the invention
In the quality for improving eggplant and increase its yield, there is huge economic benefit.
The luffa is magnetic luffa.First luffa is immersed in solion, keeps its adsorption certain
Metal cation helps to activate cellulase, improves enzymatic activity, shortens the compost time.
The activated biomass is humus, yolk, at least two in six ring stones.Humus is organic matter through microorganism
The colloidal substance that decomposition and inversion is formed, generally black or burgundy, are the chief component of the soil organism, humus master
It to be made of nutrients such as carbon, hydrogen, oxygen, nitrogen, sulphur, phosphorus, humus has the cohesiveness of appropriateness, is to form crumb structure good
Good cementing agent, secondly, the antimicrobial effect of eating of humus energy, it is horizontal to keep the soil organism, but also help guarantee nitrogen
With other required nutrition, these all exist in humus complexs combination of antidecomposition.The part turned to be yellow inside yolk, that is, egg,
Rich in microelements such as liposoluble vitamin, monounsaturated fatty acids, phosphatide, phosphorus, iron, yolk is in addition to being rich in nutrient, energy
It dramatically increases outside the content of organic matter in soil, the phosphatide being rich in has certain protective effect to microorganism, can be improved micro-
The activity of biology.Six ring stones are the condensates with four big energy: energy one, the far infrared that launch wavelength is 8-15 μm are made
For water, can activated water molecule, so that water clusters is become smaller, be more suitable for soil absorption, act on microorganism, ferment is promoted to generate,
Activate microbial cell；Redox reaction, and coupling collar easily occur for energy two, permanent generating negative ions, variable valency metal Fe
Hydrone in border forms a large amount of anion, has the function of inhibiting or killing soil-borne disease bacterium；Energy three, six ring stones contain
The nutrients such as microelement " germanium " and calcium, magnesium, sodium, potassium, iron, selenium, zinc improve soil ion type；Energy four generates biology
Electricity.
The N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea.Improve soil using plant ash
And supplement potash fertilizer, calcium superphosphate supplement phosphate fertilizer, urea supplements nitrogenous fertilizer.
The plant ash, calcium superphosphate, urea adding proportion (by mass) be 5~7:1:4.
The bio-bacterial manure is for inhibiting eggplant root rot.
A kind of preparation method for the bio-bacterial manure preventing and treating eggplant root rot, comprising the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, bent smart fermentation liquid, shaken cultivation is added in luffa；
S3, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
The present invention compared with the prior art in, will each raw material mix after carry out compost fermentation together, have the advantages: first
Qu Jingyu luffa is co-cultured, ferment compost is added, so that the microorganism of Qu Jingzhong be made to come into full contact with luffa, is adsorbed
In the porous structure of luffa, and using luffa as nutriment, the degradation such as eccrine fiber element enzyme, pectase, carbohydrase is fine
Tie up the carbohydrate that element is small molecule and secrete some biotic factors etc., nutrient is provided for subsequent ferment Microorganism colonization,
It is secondary, luffa is modified by the degradation of enzyme, after enzymatic hydrolysis, a large amount of pectin, cellulose dissolution, lignin are also gone
It removes, surface is relatively rough, and very Big mutation rate occurs for hole, and it is small to one nanometer, greatly to tens nanometers or even some tens of pm, become micro-
Biology can inhabit the microenvironment of life, be conducive to the attachment of subsequent ferment microorganism and non-pathogenic fusarium fermented liquid；It is another
The unformed partial hydrolysis of aspect fiber makes the hole of crystal region become larger, and helps to improve water-retaining property and water absorption.
The present invention is fixed to luffa surface by the microorganism that luffa adsorbs ferment and Qu Jingzhong, and one improves
Biomass, microorganism is stable and not easily runs off, and resistance to murder by poisoning ability is strong, and two carry out surface aggregation microorganism under aerobic/anoxia condition
Organic matter in degradation soil, increases the content of available N and P, potassium, sulphur etc. in soil, increases ASI systematic approach, while interior
The microbial degradation loofah fiber in portion is bred for the microbe survival on surface, and the activity of being kept fixed microorganism is unattenuated,
Until luffa degradation is finished, the microorganism Spontaneous release of absorption increases diversity of soil microorganism into soil.
The luffa is magnetic luffa, the preparation method comprises the following steps: deionized water 100g, Fe is added in the reactor3O4
0.2g、MgSO4 0.15g、MnSO4 0.15g、CoCl2 0.2g、ZnSO4Sponge gourd is added at suspension in 0.2g, ultrasonic disperse
Network is ultrasonically treated mixed solution, then filters, is dry, obtaining magnetic luffa.
The condition of the step S2 shaken cultivation are as follows: 25~37 DEG C, 40~100rpm, 48~72h of shaken cultivation.
The condition of the step S3 compost fermentation are as follows: first aerobic fermentation 2d, subsequent 20~30d of decomposed fermentation, during which every 2d
Turning is primary.
A kind of application for the bio-bacterial manure preventing and treating eggplant root rot, the bio-bacterial manure is for inhibiting eggplant root rot.
One, embodiment
Embodiment 1
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: luffa 100g, bent essence 10g,
Ferment 20g, activated biomass 20g (humus 10g, yolk 10g), N P and K ternary compound fertilizer 35g, non-pathogenic fusarium bacterium
10g, the N P and K ternary compound fertilizer mix by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urea
Adding proportion (by mass) is 6:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, bent smart fermentation liquid, 30 DEG C, 70rpm, shaken cultivation 60h is added in luffa；
S3, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 25d, and during which every 2d turning is primary；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Embodiment 2
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: magnetic luffa 50g, bent essence
2g, ferment 10g, activated biomass 10g (humus 5g, yolk 5g), N P and K ternary compound fertilizer 50g, non-pathogenic fusarium bacterium
5g, the N P and K ternary compound fertilizer mix by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urea
Adding proportion (by mass) is 5:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, it prepares magnetic luffa: deionized water 100g, Fe being added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, bent smart fermentation liquid, 25 DEG C, 40rpm, shaken cultivation 72h is added in magnetic luffa；
S4, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 30d, and during which every 2d turning is primary；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Embodiment 3
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: magnetic luffa 80g, bent essence
6g, ferment 15g, activated biomass 15g ((humus 8g, yolk 7g)), N P and K ternary compound fertilizer 42g, non-pathogenic fusarium
Bacterium 8g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urea
Adding proportion (by mass) be 6:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, it prepares magnetic luffa: deionized water 100g, Fe being added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO40.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, bent smart fermentation liquid, 28 DEG C, 60rpm, shaken cultivation 66h is added in magnetic luffa；
S4, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 28d, and during which every 2d turning is primary；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Embodiment 4
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: magnetic luffa 100g, bent essence
10g, ferment 20g, activated biomass 20g (humus 10g, yolk 10g), N P and K ternary compound fertilizer 35g, non-pathogenic fusarium
Bacterium 10g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urea
Adding proportion (by mass) be 6:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, it prepares magnetic luffa: deionized water 100g, Fe being added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO4 0.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, bent smart fermentation liquid, 30 DEG C, 70rpm, shaken cultivation 60h is added in magnetic luffa；
S4, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 25d, and during which every 2d turning is primary；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Embodiment 5
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: magnetic luffa 120g, bent essence
15g, ferment 25g, activated biomass 25g (humus 15g, six ring stone 10g), N P and K ternary compound fertilizer 27g, non-pathogenic sickle
Spore bacterium 13g, the N P and K ternary compound fertilizer are mixed by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urine
The adding proportion (by mass) of element is 6:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, it prepares magnetic luffa: deionized water 100g, Fe being added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO4 0.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, bent smart fermentation liquid, 32 DEG C, 85rpm, shaken cultivation 54h is added in magnetic luffa；
S4, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 23d, and during which every 2d turning is primary；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Embodiment 6
A kind of bio-bacterial manure preventing and treating eggplant root rot, the raw material comprising following parts by weight: magnetic luffa 150g, bent essence
20g, ferment 30g, activated biomass 30g (yolk 15g, six ring stone 15g), N P and K ternary compound fertilizer 20g, non-pathogenic fusarium
Bacterium 15g.The N P and K ternary compound fertilizer is mixed by plant ash, calcium superphosphate, urea, plant ash, calcium superphosphate, urea
Adding proportion (by mass) be 7:1:4；Preparation method includes the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of the left side 0.5~2cm
Right column；
S2, it prepares magnetic luffa: deionized water 100g, Fe being added in the reactor3O4 0.2g、MgSO4 0.15g、
MnSO4 0.15g、CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, surpass to mixed solution at suspension
Then sonication filters, is dry, obtaining magnetic luffa；
S3, bent smart fermentation liquid, 35 DEG C, 100rpm, shaken cultivation 48h is added in magnetic luffa；
S4, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei raw
On the luffa of object, activated biomass is added and is uniformly mixed, adjust water content 50~70%, first aerobic fermentation 2d is then decomposed
Ferment 20d, and during which every 2d turning is primary；
S5, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S6, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
Two, experimental example
It is common vegetable field soil, basic physical and chemical pH6.3, organic matter 20.2g/kg, full nitrogen for examination soil
1.62g/Kg, full phosphorus 0.61g/Kg, full potassium 24.5g/Kg, using pot experiment, every basin fills soil 30kg, plants 4 plants of eggplant, Miao Zuo
Spacing under upper right is 15cm, and test is divided into 8 processing, and 10 repetitions are arranged in each processing；Conventional method pipe is planted by eggplant
Reason measures the influence after its influence and final result to eggplant growing state to eggplant fruit mass before blooming to eggplant,
And repeat to do pot experiment according to above-mentioned experiment, it is inoculated with eggplant fusarium solani, a situation arises for observation eggplant root rot.
Comparative example 1: not applying fertilizer, and is control group；
Comparative example 2: applying N, P, K fertilizer, and amount of application is urea 16g/ basin, and Diammonium phosphate (DAP) 30g/ basin, potassium phosphate is 10g/ basin；
Embodiment 1-6: dose be 40g/ basin, living bacteria count >=300,000,000/gram；
1 different disposal of table is grown to eggplant and the influence of yield
A situation arises to eggplant root rot for 2 different disposal of table
Upper table 1 is the manure trial of embodiment 1-6, comparative example 1-2, it can be deduced that the eggplant of bio-bacterial manure processing of the invention
The total output and contrast ratio of son have different degrees of increase, and the eggplant fruit mass of Processing Example 4 improves at most, reaches
136g, less fertilising control group improve 43.2%；The each measurement index analysis of eggplant plant height, stem thickness, root fresh weight is obvious to be compared
The production effect of ratio 1-2 is good, shows that applying bio-bacterial manure processing has different degrees of facilitation to eggplant growth；Reason
It is to apply bio-bacterial manure to improve the character of soil fertility and eggplant plant, keeps soil nutrient sufficient, crop absorbs greatly
The nutriment of amount, to make to improve upgrowth situation, yield increases.
Upper table 2 is embodiment 1-6, the epidemic prevention and control of comparative example 1-2 is tested, it can be deduced that bio-bacterial manure of the invention is to eggplant
Sub- root rot has good control efficiency, reduces the generation of eggplant pest and disease damage, improves eggplant quality.
Claims (10)
1. a kind of bio-bacterial manure for preventing and treating eggplant root rot, which is characterized in that the raw material comprising following parts by weight: luffa 50~
It is 150g, bent 2~20g of essence, 10~30g of ferment, 10~30g of activated biomass, 20~50g of N P and K ternary compound fertilizer, non-pathogenic
5~15g of property Fusariumsp.
2. bio-bacterial manure according to claim 1, which is characterized in that the luffa is magnetic luffa.
3. bio-bacterial manure according to claim 1, which is characterized in that the activated biomass is humus, yolk, six rings
At least two in stone.
4. bio-bacterial manure according to claim 1, which is characterized in that the N P and K ternary compound fertilizer is by plant ash, mistake
Calcium phosphate, urea mix.
5. bio-bacterial manure according to claim 4, which is characterized in that the adding proportion of plant ash, calcium superphosphate, urea
It (by mass) is 5~7:1:4.
6. a kind of preparation method of bio-bacterial manure as described in claim 1, which comprises the following steps:
S1, by mature loofah peeling, deseeding, cut both ends narrower portion, remainder is cut into a thickness of 0.5~2cm's or so
Column；
S2, bent smart fermentation liquid, shaken cultivation is added in luffa；
S3, ferment fermentation liquid, non-pathogenic fusarium fermented liquid are uniformly sprayed onto step S2 to load there is Qu Jingwei biology
On luffa, activated biomass is added and is uniformly mixed, adjusts water content 50~70%, compost fermentation；
S4, the material heap that N P and K ternary compound fertilizer is added to step S3, are sufficiently stirred, stack 5~7 days naturally；
S5, moisture is evaporated to water content be 30~40% when, be granulated, pack, quality inspection, qualified product sealing storage.
7. the preparation method of bio-bacterial manure according to claim 6, which is characterized in that the luffa is magnetic sponge gourd
Network, the preparation method comprises the following steps: deionized water 100g, Fe is added in the reactor3O4 0.2g、MgSO4 0.15g、MnSO4 0.15g、
CoCl2 0.2g、ZnSO40.2g, ultrasonic disperse are added luffa, are ultrasonically treated to mixed solution, then at suspension
Filtering, drying, obtain magnetic luffa.
8. the preparation method of bio-bacterial manure according to claim 6, which is characterized in that the item of the step S2 shaken cultivation
Part are as follows: 25~37 DEG C, 40~100rpm, 48~72h of shaken cultivation.
9. the preparation method of bio-bacterial manure according to claim 6, which is characterized in that the item of the step S3 compost fermentation
Part are as follows: first aerobic fermentation 2d, 20~30d of subsequent decomposed fermentation, during which every 2d turning is primary.
10. a kind of application of bio-bacterial manure as claimed in any one of claims 1 to 5, which is characterized in that the bio-bacterial manure is used
In inhibition eggplant root rot.
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