CN108892580A - A kind of more bacterium Composite microbial inoculum fertilizers - Google Patents
A kind of more bacterium Composite microbial inoculum fertilizers Download PDFInfo
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention discloses a kind of more bacterium Composite microbial inoculum fertilizers, which is prepared from the following raw materials：Sugar residue 25%-40%, crop material 25%-40%, potassium fulvate 5%-8%, dregs of beans 15%-25%, crushed crude pearlite 8%-15% and microbial inoculum particle 2%-5%, a kind of more bacterium Composite microbial inoculum fertilizers provided by the invention carry out production preparation by using agricultural fertilizer, it can effectively improve fertility, production cost is reduced simultaneously, and the microbial inoculum particle by being prepared separately is come fermentation fertilizer, bacterial activity can be effectively ensured, improve fertilizer and save market, guarantee fertilization effect.
Description
Technical field
The present invention relates to technical field of fertilizers more particularly to a kind of more bacterium Composite microbial inoculum fertilizers.
Background technique
In present agricultural planting and production, fertilizer using more and more common, and recently as organic fertilizer
The development of technology, partial region have the potentiality instead of inorganic fertilizer, can be effectively improved soil texture, avoid hardened.
But existing bacteria agent fertilizer, when producing preparation, the raw material proportioning of selection is complicated, it is impossible to ensure that fertilizer
Fertility, but also the control of inconvenient preparation process are easy to increase production cost, while the microbial bacterial agent preparation process used
Complexity, microbial activity is lower, be easy to cause fertilizer to absorb insufficient, influences fertilization effect.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of more bacterium complex microorganisms proposed
Microbial inoculum fertilizer.
To achieve the goals above, present invention employs following technical solutions：
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 25%-40%, crop material 25%-40%, potassium fulvate 5%-8%, dregs of beans 15%-25%, crushed crude pearlite 8%-15%
With microbial inoculum particle 2%-5%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, and ventilation time is 30 minutes to 45
Minute.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 15%-25%, bacillus megaterium 15%-25%, glue
Freeze sample bacillus 5%-10%, rice aspergillus 3%-8%, colloid bacillus cereus 25%-40% and Streptomyces jingyangensis 25%-
40%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 5%-10%, peptone 5%-10%, sodium chloride 3%-6%, fine jade
Rouge 15%-25% and water 40%-60%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
A kind of more bacterium Composite microbial inoculum fertilizers provided by the invention carry out production preparation by using agricultural fertilizer, can
To effectively improve fertility, while production cost is reduced, and the microbial inoculum particle by being prepared separately is come fermentation fertilizer, it can be effective
Guarantee bacterial activity, improves fertilizer and save market, guarantee fertilization effect.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this
Invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, not
For limiting the present invention.
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 25%-40%, crop material 25%-40%, potassium fulvate 5%-8%, dregs of beans 15%-25%, crushed crude pearlite 8%-15%
With microbial inoculum particle 2%-5%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, ventilation time is 30 minutes
To 45 minutes.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 15%-25%, bacillus megaterium 15%-
25%, bacillusmusilaginosiengineering 5%-10%, rice aspergillus 3%-8%, colloid bacillus cereus 25%-40% and Streptomyces jingyangensis
25%-40%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 5%-10%, peptone 5%-10%, sodium chloride 3%-
6%, agar 15%-25% and water 40%-60%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
A kind of more bacterium Composite microbial inoculum fertilizers provided by the invention carry out production preparation by using agricultural fertilizer, can
To effectively improve fertility, while production cost is reduced, and the microbial inoculum particle by being prepared separately is come fermentation fertilizer, it can be effective
Guarantee bacterial activity, improves fertilizer and save market, guarantee fertilization effect.
Embodiment 1
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 27%, crop material 32%, potassium fulvate 8%, dregs of beans 22%, crushed crude pearlite 8% and microbial inoculum particle 3%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, ventilation time is 30 minutes
To 45 minutes.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 15%, bacillus megaterium 15%, gel-shaped bud
Spore bacillus 6%, rice aspergillus 5%, colloid bacillus cereus 32% and Streptomyces jingyangensis 27%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 7%, peptone 7%, sodium chloride 4%, agar 22% and water
60%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
Embodiment 2
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 33%, crop material 30%, potassium fulvate 5%, dregs of beans 18%, crushed crude pearlite 10% and microbial inoculum particle 4%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, ventilation time is 30 minutes
To 45 minutes.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 20%, bacillus megaterium 17%, gel-shaped bud
Spore bacillus 6%, rice aspergillus 3%, colloid bacillus cereus 25% and Streptomyces jingyangensis 29%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 10%, peptone 10%, sodium chloride 6%, 18% and of agar
Water 56%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
Embodiment 3
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 29%, crop material 32%, potassium fulvate 6%, dregs of beans 18%, crushed crude pearlite 10% and microbial inoculum particle 5%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, ventilation time is 30 minutes
To 45 minutes.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 20%, bacillus megaterium 15%, gel-shaped bud
Spore bacillus 6%, rice aspergillus 4%, colloid bacillus cereus 25% and Streptomyces jingyangensis 30%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 6%, peptone 6%, sodium chloride 3%, agar 25% and water
60%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
Embodiment 4
A kind of more bacterium Composite microbial inoculum fertilizers, more bacterium Composite microbial inoculum fertilizers are prepared from the following raw materials：Sugar
Slag 30%, crop material 32%, potassium fulvate 5%, dregs of beans 15%, crushed crude pearlite 15% and microbial inoculum particle 3%.
Preferably, more bacterium Composite microbial inoculum fertilizers include the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, in 500 rpms to 600 rpms rates
Under, uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats to 80 degrees Celsius to 95 degrees Celsius originally, heating time 30
Minute, sterilization processing then cooled to room temperature, and obtains raw bactericidal material to 45 minutes.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added,
And a certain amount of water is added, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, obtain
Fermentation raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary,
Fermentation temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind progress drying and processing to water content is
8%-12% obtains drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, then crosses 150 mesh to 180 mesh screen mistakes
Filter, obtains fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
Preferably, the ventilation temperature of the S7 step is 30 degrees Celsius to 35 degrees Celsius, ventilation time is 30 minutes
To 45 minutes.
Preferably, the sugar residue includes one or more mixtures of corn sugar residue, malt sugar residue and Klinit dregs.
Preferably, the microbial inoculum particle includes bacillus subtilis 15%, bacillus megaterium 20%, gel-shaped bud
Spore bacillus 5%, rice aspergillus 5%, colloid bacillus cereus 30% and Streptomyces jingyangensis 25%.
Preferably, the preparation method of the microbial inoculum particle includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to
Room temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, under 30 rpms to 45 rpms rates
Stirring, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, sterile water is added, then sodium alginate is being added in oscillation stirring
It with POLYPROPYLENE GLYCOL solution, is cultivated, obtains culture solution.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid in batches
In solution, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
Preferably, the microbial inoculum carrier includes beef extract 8%, peptone 6%, sodium chloride 4%, agar 22% and water
60%.
Preferably, the concentration of the sodium alginate and POLYPROPYLENE GLYCOL is 5%-8%.
Preferably, the crosslinking curing temperature is 35 degrees Celsius to 38 degrees Celsius.
Claims (9)
1. a kind of more bacterium Composite microbial inoculum fertilizers, it is characterised in that：More bacterium Composite microbial inoculum fertilizers are by following original
Material is made：Sugar residue 25%-40%, crop material 25%-40%, potassium fulvate 5%-8%, dregs of beans 15%-25%, crushed crude pearlite
8%-15% and microbial inoculum particle 2%-5%.
2. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 1, it is characterised in that：More bacterium are compound micro-
Bacteria agent fertilizer includes the following steps：
S1, weighing：Sugar residue, crop material and dregs of beans are weighed according to mass percent, it is spare.
S2, crushing：The raw material of weighing is added separately to inside pulverizer, pulverization process is carried out, obtains raw material powder.
S3, mixing：Raw material powder is added to inside high speed mixer, under 500 rpms to 600 rpms rates,
Uniform stirring 30 minutes to 45 minutes, obtain mixed raw material.
S4, pretreatment：Mixing was put into oven heats originally to 80 degrees Celsius to 95 degrees Celsius, heating time is 30 minutes
To 45 minutes, sterilization processing was then cooled to room temperature, and obtained raw bactericidal material.
S5, addition：Raw bactericidal material is put into blender, potassium fulvate, crushed crude pearlite and microbial inoculum particle is then added, and add
Enter a certain amount of water, under 120 rpms to 150 rpms rates, uniform stirring 15 minutes to 20 minutes, is fermented
Raw material.
S6, fermentation：Fermentation raw material is placed into inside fermentor and is fermented, keeps every 12 hours stirring stirrings primary, fermentation
Temperature is 50 degrees Celsius to 55 degrees Celsius, and fermentation time is 3 days to 5 days, obtains fermenting mixture.
S7, dehydration：Fermenting mixture is added to inside drying box, being passed through hot wind and carrying out drying and processing to water content is 8%-
12%, obtain drying composite.
S8, crushing：Pulverizer is added in drying composite and carries out pulverization process, 150 mesh is then crossed to 180 mesh net filtrations, obtains
To fertilizer powder.
S9：It is granulated：Fertilizer powder is added to pelletizer, is granulated, then carrying out packaging can be obtained finished product fertilizer.
3. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 2, it is characterised in that：The S7 step is led to
Air temperature is 30 degrees Celsius to 35 degrees Celsius, and ventilation time is 30 minutes to 45 minutes.
4. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 1, it is characterised in that：The sugar residue includes jade
One or more mixtures of rice sugar residue, malt sugar residue and Klinit dregs.
5. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 1, it is characterised in that：The microbial inoculum particle packet
Include bacillus subtilis 15%-25%, bacillus megaterium 15%-25%, bacillusmusilaginosiengineering 5%-10%, rice aspergillus
3%-8%, colloid bacillus cereus 25%-40% and Streptomyces jingyangensis 25%-40%.
6. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 5, it is characterised in that：The microbial inoculum particle
Preparation method includes the following steps：
P1, microbial inoculum carrier is first heated to 120 degrees Celsius to 130 degrees Celsius, carries out sterilization treatment, then naturally cools to room
Temperature obtains sterilant carriers.
P2, sterilant carriers are mixed with strain, is added in agitator tank, stirred under 30 rpms to 45 rpms rates
It mixes, uniform stirring 5 minutes to 10 minutes, obtains mixed culture medium.
P3, mixed culture medium is added in culture dish, addition sterile water, oscillation stirring, then sodium alginate is being added and is gathering
Propylene alcoholic solution, is cultivated, and culture solution is obtained.
P4, by culture solution by magnetic agitation it is uniform after, be added rapidly to sterile calcium chloride and sterile boric acid solution in batches
In, crosslinking curing is carried out, gel ball is obtained.
P5, gel ball progress frozen dried is taken out, microbial inoculum particle can be obtained.
7. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 6, it is characterised in that：The microbial inoculum carrier packet
Include beef extract 5%-10%, peptone 5%-10%, sodium chloride 3%-6%, agar 15%-25% and water 40%-60%.
8. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 6, it is characterised in that：The sodium alginate and
The concentration of POLYPROPYLENE GLYCOL is 5%-8%.
9. according to a kind of more bacterium Composite microbial inoculum fertilizers of the claim 2, it is characterised in that：The crosslinking curing temperature
Degree is 35 degrees Celsius to 38 degrees Celsius.
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| CN113527002A (en) * | 2021-04-29 | 2021-10-22 | 滨州市京阳生物肥业有限公司 | Preparation method of biological organic fertilizer for preventing and treating cotton verticillium wilt |
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