CN108848732A - A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica - Google Patents
A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica Download PDFInfo
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Abstract
本发明公开一种沙漠原位微生物覆膜技术及其联合沙柳的绿色生态治理方法,其包括步骤:对待治理沙漠区进行区域分割,将所述待治理沙漠区分割为多个植被种植区与多个微生物覆膜沙土区;将沙漠自源菌CGMCC No.15633种子活化,逐级扩大培养;在多个微生物覆膜沙土区内分别喷洒沙漠自源生物胶结剂,每个微生物覆膜沙土区在沙漠自源生物胶结剂的作用下形成微生物覆膜层,固定所述待治理沙漠区的表层沙土;在所述多个植被种植区内分别种植多棵沙柳,每个植被种植区在相应的沙柳作用下形成植被层,固定所述待治理沙漠区的下部沙土层。本发明能对沙漠土进行原位覆膜,防止外来生物入侵压制和排挤本地生物,真正做到以沙治沙的目的。
The invention discloses an in-situ microbial coating technology for deserts and a green ecological treatment method combined with salix salix, which comprises the steps of: dividing the desert area to be treated into regions, dividing the desert area to be treated into multiple vegetation planting areas and Multiple microbial-coated sandy soil areas; activate the seeds of the desert-derived fungus CGMCC No.15633, and expand the cultivation step by step; spray the desert-derived bio-cementing agent in multiple microbial-coated sandy areas, and each microbial-coated sandy area Under the action of the desert's self-derived biological cement, a microbial coating layer is formed to fix the surface sand in the desert area to be controlled; in the multiple vegetation planting areas, a plurality of salix willows are respectively planted, and each vegetation planting area is in a corresponding The vegetation layer is formed under the action of Salix salix, and the lower sand layer of the desert area to be controlled is fixed. The invention can cover the desert soil in situ, prevent foreign organisms from invading, suppressing and crowding out local organisms, and truly achieve the purpose of sand control.
Description
技术领域technical field
本发明涉及一种沙漠的绿色生态治理方法,尤其涉及一种沙漠原位微生物 覆膜技术及其联合沙柳的绿色生态治理方法。The invention relates to a green ecological management method of deserts, in particular to a desert in-situ microbial coating technology and a green ecological management method combined with Salix salix.
背景技术Background technique
在全球各地因风沙问题引起的沙化、侵蚀现象造成了生物的减少以及环境 的破坏,直接或间接的对国家和社会造成了重大经济损失和严重生态问题。因 此,各国在防风固沙领域都投入了大量的人力和物力,目的在于创新沙漠治理 机制,也因此收到了较好的成效。通过对沙漠的治理不仅能够改善生态环境和 农牧业生产条件,更重要的是对促进经济结构调整和社会进步也具有重要意义。The desertification and erosion caused by wind and sand all over the world have caused the reduction of organisms and the destruction of the environment, directly or indirectly causing major economic losses and serious ecological problems to the country and society. Therefore, all countries have invested a lot of manpower and material resources in the field of windbreak and sand fixation, with the purpose of innovating the desert governance mechanism, which has received good results. The management of deserts can not only improve the ecological environment and agricultural and animal husbandry production conditions, but more importantly, it is also of great significance to promote economic structural adjustment and social progress.
沙漠风沙土是风成砂性母质上发育起来的特殊性土壤,颗粒细小、磨圆度 较好、粒间无粘聚力、松散、易流动、易风蚀。国内外有诸多学者对于沙漠荒 漠化治理控制治理问题进行了深入的研究,取得了较好的成果。如,通过研究 大气氮沉降、微生物菌落和沙漠植被三者之间的相互影响机理,寻求助长沙漠 植被的方法,发现氮素的沉降量增加,使得沙漠中养分增加,微生物富集,利 于植被的生长,依据沙漠景观建立概念模型研究对生物土壤结皮分布的地貌控 制等。公开号CN106717226A的专利申请公开了一种应用秸秆草毯进行沙漠绿 化方法,利用秸秆草毯对沙漠进行治理,秸秆草毯内有草种、树种、花种、菜种等,并进行混播,这些种子生根、发芽,可以改善沙漠的绿化和生态。公开号 CN206118663U的专利申请公开了一种沙地植物生态治理及其装置,包括圆环, 所述圆环外侧连接有集水叶片,结构简单,操作方便,为可降解塑料制成,降 解后的塑料还可以为植物提供养料,装置安装方便,不会被风吹走,沙体流动 幅度不大时也可以维持在沙地表面,不轻易破坏,值得推广。以上发明中治理 模式是进行植被防护,植被防护的工作体现在对植物物种的选择上,不足之处 在于完全依靠植物根系的包裹,因沙漠气候条件使得绿色植被成活困难,即使 成活也只能使流动沙丘变成半固定沙丘,未能从根本上截断沙尘的源头,同时 植物固沙会造成自然风场的风向发生改变,扰动的风场在风速减小的区域出现 沙尘的再搬运与再堆积。Desert aeolian soil is a special soil developed on the parent material of aeolian sand, with fine particles, good roundness, no cohesion between particles, loose, easy to flow, and easy to wind erosion. Many scholars at home and abroad have conducted in-depth research on desertification control and governance issues, and achieved good results. For example, through the study of the interaction mechanism among atmospheric nitrogen deposition, microbial colonies and desert vegetation, we sought ways to promote desert vegetation. It was found that the increase in nitrogen deposition will increase the nutrients in the desert and enrich the microorganisms, which is conducive to the growth of vegetation. growth, based on the desert landscape to establish a conceptual model to study the geomorphic control on the distribution of biological soil crusts, etc. The patent application with the publication number CN106717226A discloses a method for desert greening by using a straw blanket, which is used to control the desert. There are grass seeds, tree species, flower seeds, vegetable seeds, etc. in the straw blanket, and they are mixed. The seeds take root and germinate, which can improve the greening and ecology of the desert. The patent application with publication number CN206118663U discloses a kind of sandy land plant ecological management and its device, including a ring, the outside of which is connected with water-collecting blades, simple in structure, easy to operate, made of degradable plastic, and the degraded Plastic can also provide nutrients for plants. The device is easy to install and will not be blown away by the wind. It can also be maintained on the surface of the sand when the flow of the sand body is not large, and it is not easy to damage. It is worth promoting. The management mode in the above invention is to carry out vegetation protection. The work of vegetation protection is reflected in the selection of plant species. The disadvantage is that it completely depends on the package of plant roots. Because of desert climate conditions, it is difficult for green vegetation to survive. Even if it survives, it can only be used. Mobile sand dunes become semi-fixed dunes, which fail to fundamentally cut off the source of sand and dust. At the same time, plant sand fixation will cause the wind direction of the natural wind field to change, and the disturbed wind field will re-transport and re-transport sand and dust in the area where the wind speed decreases. accumulation.
采用化学加固也是沙漠工程中常用的治理措施,公开号CN 101548595 B 的专利申请公开了基于有机复合材料的化学固沙绿化技术的沙漠治理方法,在 撒播植生用绿化物的沙或土层上,喷洒亲水性聚氨酯树脂固化剂溶液,形成多 孔质固化沙层,在没有丰富的地下水源、强风蚀的沙漠特有环境下,可以对沙 进行稳定、有效的固化,实现植物绿化功能和对荒漠化土地的生态恢复及治理。 公开号CN106348872A的专利申请公开了改良沙化土质的土壤治理肥料及其制 备方法,使用制备而成的改良沙化土质的土壤治理肥料能有效提高土壤肥力、降 低沙化程度。但利用化学建材的灌浆来加固土壤,会带来严重的水土污染,对 环境造成不良影响。The use of chemical reinforcement is also a commonly used control measure in desert engineering. The patent application with publication number CN 101548595 B discloses a desert control method based on chemical sand-fixing greening technology based on organic composite materials. On the sand or soil layer where greening materials are sown, spray Hydrophilic polyurethane resin curing agent solution forms a porous solidified sand layer. In the desert-specific environment without abundant groundwater sources and strong wind erosion, it can solidify the sand stably and effectively, realize the function of plant greening and protect the desertified land. Ecological restoration and governance. The patent application of publication number CN106348872A discloses soil control fertilizer for improving desertified soil quality and preparation method thereof, and using the prepared soil control fertilizer for improving sandy soil quality can effectively improve soil fertility and reduce the degree of desertification. However, the use of chemical building materials for grouting to reinforce the soil will cause serious water and soil pollution and cause adverse effects on the environment.
有机物质复合材料也是一种防沙治理手段,公开号CN106358482A的专利 申请公开了一种治理沙漠、沙地的方法,可通过人力或机械向沙漠、沙地表面 以任意顺序洒水和撒固态有机物质,或者直接洒由固态有机物质形成的具有粘 结性及粘附性的溶液,利用搅拌装置将洒有固态有机物质形成的溶液或撒有固 态有机物质和洒水后的沙漠、沙地表面沙体拌匀,使沙漠、沙地表层的沙体改 性。但降解产物具有一定的毒副作用,会给周边生态带来危害。公开号 CN106947492A的专利申请公开了一种复合固沙模型及其制备方法和应用,通 过先在场地上喷播纤维丝,然后在其表面喷洒生物加固液,微生物加固液在沙 土表层及内部结硬,结合纤维丝共同形成加筋结构,克服了原有微生物固沙方 法表层脆性大、耐久性差、易风蚀的缺点,但其使用的生物加固液为外来菌种, 生长难以控制,破坏原生态系统的结构和工程,导致原有生物群落的衰退和消 失,可能对环境造成不可逆转的污染。Organic matter composite materials are also a means of sand control. The patent application with publication number CN106358482A discloses a method for controlling deserts and sandy lands. Water and solid organic matter can be sprinkled on the surface of deserts or sandy lands in any order by manpower or machinery. , or directly sprinkle a cohesive and adhesive solution formed by solid organic substances, use a stirring device to sprinkle the solution formed by solid organic substances or sprinkle solid organic substances and sprinkle sand on the surface of deserts and sandy land Mix well to modify the sand bodies on the surface of deserts and sandy lands. However, the degradation products have certain toxic and side effects, which will bring harm to the surrounding ecology. The patent application with the publication number CN106947492A discloses a composite sand-fixing model and its preparation method and application. By first spraying fiber filaments on the field, and then spraying the biological reinforcement liquid on the surface, the microbial reinforcement liquid will harden on the surface and inside of the sand, combined with Fiber filaments together form a reinforced structure, which overcomes the shortcomings of the original microbial sand fixation method, such as high surface brittleness, poor durability, and easy wind erosion. Engineering, leading to the decline and disappearance of the original biological community, may cause irreversible pollution to the environment.
发明内容Contents of the invention
为了解决现有技术中存在的问题,本发明提出一种沙漠原位微生物覆膜技 术及其联合沙柳的绿色生态治理方法。用筛选自待治理区沙漠的自源菌种,对 沙漠土进行原位覆膜,防止外来生物入侵压制和排挤本地生物,真正做到以沙 治沙的目的。在此技术基础上联合耐旱、抗风蚀、耐沙埋、生长迅速、根茎发 达、防风固沙、保持水土功效显著的植物沙柳固定微生物覆膜层下沙土,为沙 漠治理提供良好的绿色治理联动结构。In order to solve the problems existing in the prior art, the present invention proposes a kind of desert in-situ microbial coating technology and its green ecological management method combined with Salix salix. Use the self-sourced bacterial strains screened from the desert in the area to be treated to cover the desert soil in situ to prevent the invasion of foreign organisms from suppressing and crowding out local organisms, and truly achieve the purpose of sand control. On the basis of this technology, combined with the plant Salix salix, which is resistant to drought, wind erosion, sand burial, rapid growth, well-developed rhizomes, windbreak and sand fixation, and water and soil maintenance, to fix the sand under the microbial film layer, providing a good green governance linkage for desert governance structure.
本发明采用以下技术方案实现:一种沙漠原位微生物覆膜技术及其联合沙 柳的绿色生态治理方法,其包括步骤:The present invention adopts the following technical schemes to realize: a kind of desert in-situ microbial coating technology and its green ecological management method combined with Salix salix, which comprises the steps of:
步骤一:对待治理沙漠区进行区域分割,将所述待治理沙漠区分割为多个 植被种植区与多个微生物覆膜沙土区;Step 1: the desert area to be treated is divided into regions, and the desert area to be controlled is divided into a plurality of vegetation planting areas and a plurality of microorganism-coated sandy soil areas;
步骤二:将沙漠自源菌CGMCC No.15633种子活化,逐级扩大培养;Step 2: activate the seeds of desert self-derived bacteria CGMCC No.15633, and expand the cultivation step by step;
步骤三:在步骤一的基础上,在多个所述微生物覆膜沙土区内分别喷洒沙 漠自源生物胶结剂,每个微生物覆膜沙土区在沙漠自源生物胶结剂的作用下形 成微生物覆膜层,固定所述待治理沙漠区的表层沙土;Step 3: On the basis of step 1, the desert self-sourced bio-cementing agent is sprayed in a plurality of the microbial-coated sandy soil areas, and each microbial-coated sandy soil area forms a microbial coating under the action of the desert self-sourced bio-cementing agent. The film layer fixes the surface sand in the desert area to be controlled;
所述沙漠自源生物胶结剂包括沙漠自源菌液、胶结营养液以及胶结颗粒; 所述沙漠自源菌液由步骤二的沙漠自源菌经过扩培而成;所述胶结营养液由 CO(NH2)2、CaCl2.2H2O物质组成;所述胶结颗粒主要成分为可降解高分子吸水 聚合物;The desert self-sourced bio-cementing agent includes desert self-sourced bacterial liquid, cementing nutrient solution and cementing particles; the desert self-sourced bacterial liquid is formed by expanding the desert self-sourced bacteria in step 2; the cementing nutrient solution is made of CO (NH 2 ) 2 , CaCl 2 .2H 2 O; the main component of the cemented particles is a degradable high-molecular water-absorbing polymer;
步骤四:在所述多个植被种植区内分别种植多棵沙柳,每个植被种植区在 相应的沙柳作用下形成植被层,固定所述待治理沙漠区的下部沙土层。Step 4: plant a plurality of Salix salix in described a plurality of vegetation planting areas respectively, each vegetation planting area forms vegetation layer under the action of corresponding Salix salix, fixes the lower sand layer of described desert area to be controlled.
作为上述方案的进一步改进,多个所述微生物覆膜沙土区与多个所述植被 种植区相互交错分布。As a further improvement of the above-mentioned scheme, a plurality of the microorganism-coated sandy soil areas and a plurality of the vegetation planting areas are interlacedly distributed.
进一步地,多个所述微生物覆膜沙土区阵列式布局,每个微生物覆膜沙土 区的四角分别布局一个所述植被种植区。Further, a plurality of said microorganism-coated sand areas are arranged in an array, and the four corners of each microorganism-coated sand area are respectively arranged with a said vegetation planting area.
作为上述方案的进一步改进,所述胶结颗粒以每平方米50-70克均匀喷洒 入所述微生物覆膜沙土区内;一平方米的所述微生物覆膜沙土区喷洒5-6升沙 漠自源菌液,12-15升的所述胶结营养液。As a further improvement of the above scheme, the cemented particles are evenly sprayed into the microorganism-coated sand area at 50-70 grams per square meter; 5-6 liters of desert self-source Bacteria solution, 12-15 liters of the cemented nutrient solution.
作为上述方案的进一步改进,以所述胶结颗粒喷洒日的后一日为第一日, 在所述第一日,所述沙漠自源菌液和所述胶结营养液以体积比为1:2.5的比例交 替进行喷洒,其交替顺序为先喷洒所述沙漠自源菌液,再喷洒所述胶结营养液, 依次交替进行;所述沙漠自源菌液与所述胶结营养液交替喷洒的间隔时间为1 小时;第二、三、四日每日各自喷洒与第一日相同体积的胶结营养液。As a further improvement of the above scheme, the day after the day of spraying the cemented granules is regarded as the first day, and on the first day, the desert-derived bacterial solution and the cemented nutrient solution are in a volume ratio of 1:2.5 The proportion of spraying alternately, the alternating sequence is to spray the desert self-sourced bacterium liquid first, then spray the cementing nutrient solution, and alternately carry out successively; 1 hour; on the second, third, and fourth day, spray the same volume of cementing nutrient solution as the first day.
进一步地,所述第一日的4小时内共喷洒沙漠自源菌液与胶结营养液各2 次。Further, within 4 hours of the first day, the desert self-derived bacterial solution and the cemented nutrient solution were sprayed twice each.
进一步地,所述胶结颗粒的喷洒过程,所述沙漠自源菌液和所述胶结营养 液这两种液体的配制过程与喷洒过程,各自通过不同喷灌设备分别完成。Further, the spraying process of the cemented particles, the preparation process and the spraying process of the two liquids of the desert self-sourced bacterial liquid and the cemented nutrient solution are respectively completed by different sprinkler irrigation equipment.
作为上述方案的进一步改进,在步骤二中:将沙漠自源菌CGMCC No.15633 种子活化,再接入摇瓶、种子罐及发酵罐逐级扩大培养。As a further improvement of the above scheme, in step 2: activate the seeds of desert autogenous bacteria CGMCC No.15633, and then insert them into shake flasks, seed tanks and fermenters for gradual expansion.
作为上述方案的进一步改进,所述沙漠自源菌液由所述沙漠自源菌经过扩 培并离心后加入新鲜培养基组成,所述新鲜培养基由酵母粉20克/每升、 0.1~1molNaCl、0.05~1molNH4Cl、0.1~1molCO(NH2)2物质组成。As a further improvement of the above scheme, the desert self-sourced bacteria solution is composed of the desert self-sourced bacteria after expansion and centrifugation and adding fresh medium, and the fresh medium is composed of yeast powder 20 grams per liter, 0.1 ~ 1mol NaCl , 0.05 ~ 1mol NH 4 Cl, 0.1 ~ 1mol CO (NH 2 ) 2 material composition.
作为上述方案的进一步改进,所述胶结营养液由0.4mol~1molCO(NH2)2、 0.25mol~0.5molCaCl2.2H2O物质组成。As a further improvement of the above solution, the cementing nutrient solution is composed of 0.4mol˜1mol CO(NH 2 ) 2 , 0.25mol˜0.5mol CaCl 2 .2H 2 O substances.
本发明的优点在于:The advantages of the present invention are:
1、采用从待治理沙漠区的土壤中筛选的沙漠自源微生物(即沙漠自源菌 CGMCCNo.15633)固定待治理沙漠区的表层,形成性能稳定、强度高、保水 性强的微生物覆膜,从根本上阻断沙尘的源头,同时自源微生物的使用减少了 外来微生物对待治理沙漠区造成的影响,实现沙漠治理的原位微生物覆膜技术 在沙漠绿色生态治理中的应用;1. Use the desert-derived microorganisms screened from the soil of the desert area to be treated (that is, the desert-derived bacteria CGMCCNo.15633) to fix the surface layer of the desert area to be treated to form a microbial film with stable performance, high strength, and strong water retention. Fundamentally block the source of sand and dust, and at the same time, the use of self-sourced microorganisms reduces the impact of foreign microorganisms on the treatment of desert areas, and realizes the application of in-situ microbial coating technology for desert control in desert green ecological management;
2、采用沙漠自源微生物固定沙漠表层沙土,添加可降解胶结颗粒所形成的 微生物覆膜层具有良好的透水透气性能,可以将大气水汽或雨水等渗透到下部 沙土层,为种植区植物根系提供足够水气滋养,使得植被的成活率大大得到提 高;2. Use desert microorganisms to fix the desert surface sand and add degradable cementing particles to form a microbial coating layer that has good water permeability and air permeability, and can penetrate atmospheric water vapor or rainwater into the lower sand layer to provide plant roots in the planting area. Sufficient moisture nourishment greatly improves the survival rate of vegetation;
3、以待治理沙漠区的微生物固定沙漠表层沙土,植被根系固定沙漠底层沙 土,二者联合是在不破坏原有的沙地生态自平衡体系的基础上,将绿色微生物 技术与沙漠生态环境治理的完美结合,是沙漠生态系统的绿色治理与恢复中的 原始科技创新。3. The microorganisms in the desert area to be treated fix the desert surface sand, and the vegetation root system fixes the desert bottom sand. The combination of the two is to combine the green microbial technology with the desert ecological environment on the basis of not destroying the original sand ecological self-balancing system. The perfect combination is the original technological innovation in the green governance and restoration of the desert ecosystem.
附图说明Description of drawings
图1为对待治理沙漠区进行区域分割后示意图;Fig. 1 is the schematic diagram after the region segmentation of the desert area to be controlled;
图2为沙漠原位微生物覆膜技术及其联合沙柳的绿色生态治理沙漠治理效 果图;Figure 2 is the desert control effect diagram of in-situ microbial mulching technology and its combination with salix salix for green ecological control of desert;
图3为微生物覆膜层固定沙漠表层土,植物固定沙漠底层土结构示意图;Fig. 3 is the fixed desert topsoil of microbial film layer, and the schematic diagram of plant fixed desert bottom soil structure;
图4为采用本发明提供的方法对微生物固定沙土层贯入阻力测试结果趋势 图。Fig. 4 is the trend chart of the test results of the penetration resistance of microbial fixed sand layer by adopting the method provided by the present invention.
具体实施方式Detailed ways
为了使本发明的目的、技术方案及优点更加清楚明白,以下结合附图及实 施例,对本发明进行进一步详细说明。应当理解,此处所描述的具体实施例仅 用以解释本发明,并不用于限定本发明。In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
实施例1Example 1
本实施例的沙漠原位微生物覆膜技术及其联合沙柳的绿色生态治理方法包 括以下步骤。The green ecological management method of the desert in-situ microbial film covering technology and its combination Salix salix of the present embodiment comprises the following steps.
步骤一:对待治理沙漠区进行区域分割,将所述待治理沙漠区分割为多个 植被种植区与多个微生物覆膜沙土区。Step 1: The desert area to be treated is divided into regions, and the desert area to be treated is divided into multiple vegetation planting areas and multiple microbial-coated sandy soil areas.
多个所述微生物覆膜沙土区与多个所述植被种植区最好相互交错分布,优 选的为多个所述微生物覆膜沙土区阵列式布局,每个微生物覆膜沙土区的四角 分别布局一个所述植被种植区。另外,多个所述微生物覆膜沙土区与多个所述 植被种植区相互交错分布,还可满足当地沙漠景观设计的需求。A plurality of said microorganism-coated sand areas and a plurality of said vegetation planting areas are preferably interlaced with each other, preferably an array layout of a plurality of said microorganism-coated sand areas, and the four corners of each microbial-coated sand area are arranged respectively One said vegetation planting area. In addition, a plurality of the microorganism-coated sandy soil areas and a plurality of the vegetation planting areas are interlacedly distributed, which can also meet the needs of local desert landscape design.
步骤二:将沙漠自源菌CGMCC No.15633种子活化,逐级扩大培养。Step 2: activate the seeds of the desert self-derived fungus CGMCC No.15633, and expand the cultivation step by step.
在本实施例中,将沙漠自源菌CGMCC No.15633种子活化,再接入摇瓶、 种子罐及发酵罐逐级扩大培养。所述菌为具有胶结固化作用的自源微生物,自 源微生物的提取防止了外来生物侵入适宜生长新区后菌群迅速繁殖导致破坏当 地生态安全。菌株gy1#,已于2018年4月18日保藏于中国微生物菌种保藏管 理委员会普通微生物中心(简称为CGMCC),保藏中心登记号为CGMCC No.15633。该菌为葡萄球菌Staphylococcus sp.。In this example, the seeds of the desert self-derived bacterium CGMCC No.15633 were activated, and then inserted into shake flasks, seed tanks and fermenters for gradual expansion. The bacteria are self-derived microorganisms with cementation and solidification effects, and the extraction of self-derived microorganisms prevents the rapid reproduction of the bacterial flora after the invasion of foreign organisms into a suitable growth area, resulting in damage to the local ecological security. The strain gy1# was deposited on April 18, 2018 in the General Microorganism Center of the China Committee for Culture Collection of Microorganisms (CGMCC for short), and the registration number of the deposit center is CGMCC No.15633. The bacterium is Staphylococcus sp.
步骤三:在步骤一的基础上,在多个所述微生物覆膜沙土区内分别喷洒沙 漠自源生物胶结剂,每个微生物覆膜沙土区在沙漠自源生物胶结剂的作用下形 成微生物覆膜层,固定所述待治理沙漠区的表层沙土。Step 3: On the basis of step 1, the desert self-sourced bio-cementing agent is sprayed in a plurality of the microbial-coated sandy soil areas, and each microbial-coated sandy soil area forms a microbial coating under the action of the desert self-sourced bio-cementing agent. The film layer fixes the sand on the surface of the desert area to be controlled.
所述沙漠自源生物胶结剂包括沙漠自源菌液、胶结营养液以及胶结颗粒。 所述沙漠自源菌液由步骤二的沙漠自源菌经过扩培而成,如所述沙漠自源菌液 由所述沙漠自源菌经过扩培并离心后加入新鲜培养基组成,所述新鲜培养基由 酵母粉20克/每升、0.1~1molNaCl、0.05~1molNH4Cl、0.1~1molCO(NH2)2物质 组成。;所述胶结营养液由CO(NH2)2、CaCl2.2H2O物质组成,如所述胶结营养 液由0.4mol~1molCO(NH2)2、0.25mol~0.5molCaCl2.2H2O物质组成。所述胶结 颗粒主要成分为可降解高分子吸水聚合物,均匀喷洒入所述微生物沙漠治理区 的土壤内。The desert-derived bio-cementing agent includes desert-derived bacterial liquid, cementing nutrient solution and cementing particles. The desert self-sourced bacteria liquid is formed by expanding the desert self-sourced bacteria in step 2. For example, the desert self-sourced bacteria liquid is composed of the desert self-sourced bacteria after expansion and centrifugation and adding fresh medium. The fresh medium is composed of 20 g/liter of yeast powder, 0.1-1 mol of NaCl, 0.05-1 mol of NH 4 Cl, and 0.1-1 mol of CO(NH 2 ) 2 . ; The cemented nutrient solution is composed of CO(NH 2 ) 2 , CaCl 2 .2H 2 O substances, for example, the cemented nutrient solution is composed of 0.4mol~1molCO(NH 2 ) 2 , 0.25mol~0.5molCaCl 2 .2H 2 O material composition. The main component of the cemented particles is a degradable high-molecular water-absorbing polymer, which is evenly sprayed into the soil of the microbial desert control area.
以所述胶结颗粒喷洒日的后一日为第一日,在所述第一日,自源菌液和胶 结营养液以体积比为1:2.5的比例交替进行喷洒,具体交替顺序为先喷洒自源菌 液,再喷洒胶结营养液,依次交替进行,所述自源菌液与胶结胶结营养液交替 喷洒的间隔时间为1小时。第二、三、四日每日各自喷洒与第一日相同体积的 胶结营养液。所述胶结颗粒的喷洒和两种胶结液的配制过程与喷洒过程各自通 过不同喷灌设备分别完成。Taking the day after the spraying day of the cemented granules as the first day, on the first day, the self-sourced bacterial solution and the cemented nutrient solution are alternately sprayed with a volume ratio of 1:2.5, and the specific alternating sequence is to spray first The self-sourced bacterial solution is then sprayed with the cementing nutrient solution alternately, and the interval between the alternate spraying of the self-sourced bacterial solution and the cemented nutrient solution is 1 hour. On the second, third, and fourth days, the same volume of cementing nutrient solution as that on the first day was sprayed each day. The spraying of the cementing particles and the preparation process and the spraying process of the two cementing liquids are respectively completed by different sprinkling equipment.
在实施过程中,所述胶结颗粒以每平方米50-70克均匀喷洒入所述微生物 覆膜沙土区内;一平方米的所述微生物覆膜沙土区喷洒5-6升沙漠自源菌液, 12-15升的所述胶结营养液。以所述胶结颗粒喷洒日的后一日为第一日,在所 述第一日,所述沙漠自源菌液和所述胶结营养液以体积比为1:2.5的比例交替进 行喷洒,其交替顺序为先喷洒所述沙漠自源菌液,再喷洒所述胶结营养液,依 次交替进行;所述沙漠自源菌液与所述胶结营养液交替喷洒的间隔时间为1小 时;第二、三、四日每日各自喷洒与第一日相同体积的胶结营养液。所述第一 日的4小时内共喷洒沙漠自源菌液与胶结营养液各2次。所述胶结颗粒的喷洒过程,所述沙漠自源菌液和所述胶结营养液这两种液体的配制过程与喷洒过程, 各自通过不同喷灌设备分别完成。In the implementation process, the cemented particles are evenly sprayed into the microorganism-coated sand area at 50-70 grams per square meter; 5-6 liters of desert self-sourced bacterial liquid are sprayed on one square meter of the microorganism-coated sand area , 12-15 liters of the cementing nutrient solution. Taking the day after the spraying day of the cemented particles as the first day, on the first day, the desert self-sourced bacterial solution and the cemented nutrient solution are alternately sprayed with a volume ratio of 1:2.5, which The alternating sequence is to spray the desert self-sourced bacterial solution first, and then spray the cemented nutrient solution, which are carried out alternately; the interval between the alternate spraying of the desert self-sourced bacterial solution and the cemented nutrient solution is 1 hour; second, On the third and fourth day, the same volume of cementing nutrient solution as that on the first day was sprayed each day. In 4 hours on the first day, spray desert self-source bacterial liquid and cementing nutrient solution respectively 2 times altogether. The spraying process of the cemented granules, the preparation process and the spraying process of the two liquids, the desert-derived bacteria liquid and the cemented nutrient solution, are respectively completed by different sprinkler irrigation equipment.
微生物覆膜层的厚度可根据环境以及治理的目标进行调整。The thickness of the microbial coating layer can be adjusted according to the environment and the goal of governance.
步骤四:在所述多个植被种植区内分别种植多棵沙柳,每个植被种植区在 相应的沙柳作用下形成植被层,固定所述待治理沙漠区的下部沙土层。Step 4: plant a plurality of Salix salix in described a plurality of vegetation planting areas respectively, each vegetation planting area forms vegetation layer under the action of corresponding Salix salix, fixes the lower sand layer of described desert area to be controlled.
沙柳为少数可以在盐碱地生长的植物之一,枝条不怕沙压,萌芽力强,根 系发达,是固定沙土的同时不破坏、不污染沙土本身的材料特性,又能固定沙 土流失、稳定水系的首选植物。至于植被层的密度,可根据环境以及治理的目 标进行调整。Salix salix is one of the few plants that can grow in saline-alkali land. Its branches are not afraid of sand pressure, have strong germination power, and a well-developed root system. It is a material characteristic that fixes sand without destroying or polluting the sand itself. It can also fix sand loss and stabilize water systems. Plants of choice. As for the density of the vegetation layer, it can be adjusted according to the environment and the goal of governance.
通过本发明提供的一种沙漠原位微生物覆膜技术及其联合沙柳的绿色生态 治理方法,对待治理沙漠区分割为多个植被种植区和多个微生物覆膜沙土区, 所述微生物覆膜沙土区在沙漠自源生物胶结剂的作用下形成微生物覆膜层,固 定所治理沙漠区的表层沙土,所述植被种植区在沙柳作用下形成植被层,固定 所述待治理沙漠区的下部沙土层,二者结合,实现对待治理沙漠区的综合治理。Through a kind of desert in-situ microbial coating technology and its green ecological management method combined with Salix salix provided by the present invention, the desert area to be treated is divided into multiple vegetation planting areas and multiple microbial coating sandy soil areas, and the microbial coating The sandy soil area forms a microbial coating layer under the action of the desert's self-sourced biological cement, and fixes the surface sand in the desert area to be controlled. The vegetation planting area forms a vegetation layer under the action of Salix salix, and fixes the lower part of the desert area to be treated. The sandy soil layer, the combination of the two, realizes the comprehensive management of the desert area to be treated.
实施例2Example 2
自然界中部分微生物在新陈代谢的过程中可诱导生成碳酸钙,能将松散的 砂土胶结成具有一定强度、渗透性良好的绿色新型人工材料。Some microorganisms in nature can induce calcium carbonate in the process of metabolism, and can cement loose sandy soil into a new green artificial material with certain strength and good permeability.
由于沙漠土壤是微生物的良好载体,利用微生物可以在多孔介质中生长、 运移和繁殖等特性,分离沙漠中具有固定、粘结效果的自源菌种,生成沙漠微 生物覆膜层固定表层沙土,使得流动沙丘经固定覆膜而成为半固定、固定沙丘, 从根本上阻断沙尘暴的源头。微生物覆膜层表现出与岩土基质亲和性、良好的 透气透水性、保水性等特点,让它成为沙漠生态治理中“会呼吸的天然屏障”。 进一步,甄选耐旱、耐贫瘠植物种植并实现其优化配置,实现原位微生物覆膜 层与沙地绿色植物间相互依存、互为依托,并共同构建沙沙漠生态系统的新格 局。Since desert soil is a good carrier of microorganisms, using the characteristics of microorganisms that can grow, migrate and reproduce in porous media, isolate the self-sourced bacteria with fixation and bonding effects in the desert, and generate a desert microbial coating layer to fix the surface sand. The mobile sand dunes become semi-fixed and fixed dunes after being covered with a fixed film, fundamentally blocking the source of sandstorms. The microbial coating layer has the characteristics of affinity with the rock-soil matrix, good air permeability and water retention, making it a "breathing natural barrier" in desert ecological management. Further, select drought-tolerant and barren-tolerant plants for planting and realize their optimal allocation, realize the interdependence and mutual support between the in-situ microbial film layer and the sandy green plants, and jointly build a new pattern of the sandy desert ecosystem.
基于以上分析,本发明提供一种沙漠原位微生物覆膜技术及其联合沙柳的 绿色生态治理方法,包括如下步骤。Based on the above analysis, the present invention provides a kind of desert in-situ microbial coating technology and its green ecological management method combined with Salix salix, comprising the following steps.
步骤一:对待治理沙漠区进行区域分割,将所述待治理沙漠区分割为多个 植被种植区2与多个微生物覆膜沙土区1。Step 1: The desert area to be treated is divided into regions, and the desert area to be controlled is divided into a plurality of vegetation planting areas 2 and a plurality of microbial film-coated sandy soil areas 1.
如图1所示,多个所述微生物覆膜沙土区1与多个所述植被种植区2互相 交错分布。多个所述微生物覆膜沙土区阵列式布局,在长宽均为10000米的区 域内每隔长宽均为2000米设置阵列式的多个所述微生物覆膜沙土区1,每个微 生物覆膜沙土区1的四角分别布局一个所述植被种植区2,所述植被种植区2 的直径为300米。As shown in Fig. 1, a plurality of said microorganism-coated sandy soil areas 1 and a plurality of said vegetation planting areas 2 are interlacedly distributed. A plurality of said microorganism-coated sandy soil areas are arranged in an array, and in an area whose length and width are 10,000 meters, a plurality of said microbial-coated sandy soil areas 1 of an array type are set every 2,000 meters in length and width. The four corners of the membrane sandy soil area 1 are respectively arranged with a vegetation planting area 2, and the diameter of the vegetation planting area 2 is 300 meters.
步骤二:所述沙漠自源菌(CGMCC No.15633)种子活化,并接入摇瓶和 罐逐级扩大培养。Step 2: The desert self-derived bacterium (CGMCC No.15633) seeds are activated, and inserted into shake flasks and tanks to expand cultivation step by step.
步骤三:对所有所述微生物覆膜区1内喷洒沙漠自源绿色生物胶结剂,形 成一定厚度的微生物覆膜层4,所述的沙漠自源生物胶结剂包括沙漠自源菌液、 胶结营养液以及胶结颗粒。Step 3: Spray desert self-sourced green bio-cementing agent in all the microbial coating areas 1 to form a certain thickness of microbial coating layer 4. The desert self-sourced bio-cementing agent includes desert self-sourced bacterial liquid, cementing nutrients liquid and cemented particles.
在所述微生物覆膜区1内喷洒可降解胶结颗粒,为使微生物固定沙土区固 定层具有良好强度、可塑性和渗透性,将胶结颗粒以每平方米50-70克均匀喷 洒入所述所有待治理微生物覆膜沙土区的土壤内,所述胶结颗粒的主要成分为 可降解高分子吸水聚合物。Spray degradable cementing particles in the microbial film-covered area 1, in order to make the fixed layer in the sandy soil area fixed by the microorganisms have good strength, plasticity and permeability, the cementing particles are evenly sprayed into all the areas to be treated with 50-70 grams per square meter. In controlling the soil in the sandy soil area covered by microorganisms, the main component of the cemented particles is a degradable high molecular water-absorbing polymer.
以保水剂即所述胶结颗粒的喷洒日的后一日为第一日,所述第一日,自源 菌液和胶结营养液以体积比为1:2.5的比例交替进行喷洒,具体为一平方米的微 生物固化区喷洒5-6升的自源菌液,12-15升的胶结营养液,交替顺序为先喷洒 自源菌液,再喷洒胶结营养液,依次交替进行,所述自源菌液与胶结营养液交 替喷洒的间隔时间为1小时,即自源菌液喷洒完一小时后喷洒胶结营养液,再 过一小时喷洒自源菌液,再过一小时喷洒胶结营养液,所述第一天4小时内共 喷洒自源菌液与胶结营养液各2次。第二、三、四日每日各自喷洒与第一日相 同体积的胶结营养液。所述胶结颗粒、自源菌液与胶结营养液的配制过程与喷洒过程各自通过不同喷灌设备分别完成。The water-retaining agent, that is, the day after the spraying day of the cemented granules is regarded as the first day, and on the first day, the self-sourced bacterial solution and the cemented nutrient solution are alternately sprayed at a volume ratio of 1:2.5, specifically one Spray 5-6 liters of self-sourced bacterial solution and 12-15 liters of cemented nutrient solution in the microbial solidification area of square meters. The interval between the alternate spraying of the bacterial solution and the cementing nutrient solution is 1 hour, that is, the cementing nutrient solution is sprayed one hour after the self-sourced bacterial solution is sprayed, the self-sourced bacterial solution is sprayed after another hour, and the cemented nutrient solution is sprayed after another hour. Spray the self-sourced bacterial solution and the cemented nutrient solution twice each within 4 hours on the first day. On the second, third, and fourth days, the same volume of cementing nutrient solution as that on the first day was sprayed every day. The preparation process and spraying process of the cemented granules, the self-sourced bacterial solution and the cemented nutrient solution are respectively completed by different sprinkler irrigation equipment.
所述的自源菌液由沙漠自源微生物经过扩培并离心后加入新鲜培养基组 成,所述新鲜培养基由酵母粉20克/每升、0.1~1molNaCl、0.05~1molNH4Cl、 0.1~1molCO(NH2)2物质组成。所述的胶结营养液由0.4mol~1molCO(NH2)2、 0.25mol~0.5molCaCl2.2H2O物质组成。The self-sourced bacteria liquid is composed of desert self-sourced microorganisms after expansion and centrifugation and adding fresh medium. The fresh medium consists of yeast powder 20 g/liter, 0.1-1molNaCl, 0.05-1molNH4Cl , 0.1-1molNH4Cl, 1molCO(NH 2 ) 2 material composition. The cementing nutrient solution is composed of 0.4mol-1mol CO(NH 2 ) 2 , 0.25mol-0.5mol CaCl 2 .2H 2 O substances.
步骤四:在所有所述植被种植区2内分别种植沙柳,分别形成一定密度的 植被层3。根据沙柳品种和数量为所有沙柳施用底肥,供给沙柳整个生长周期 内所需养分。Step 4: Plant Salix salix in all described vegetation planting areas 2 respectively, form the vegetation layer 3 of certain density respectively. Apply base fertilizer to all Salix salix according to the species and quantity of Salix salix to supply the required nutrients throughout the growth cycle of Salix salix.
所述沙柳品种选取耐旱、抗风蚀、耐沙埋、生长迅速、防风固沙、保持水 土功效显著、1~3年生无病虫害、小头直径≥0.7cm的生长健壮的沙柳枝条,在 春季进行扦插,扦插采用株行距2m×2m(约222株/亩)的配置模式,扦插前 所有所述沙柳枝条使用ABT生根粉溶液进行预处理,扦插后的2年内分别在每 年6月底之前进行一次施肥,所述施肥包括为所有沙柳施有机肥禽或畜粪便, 同时配合施氮、磷、钾肥,有机肥施用量为1.5~3kg/株;氮、磷、钾肥0.2~ 0.3kg/株。The described Salix species selects drought-resistant, wind-erosion-resistant, sand-buried, rapid-growing, wind-proof and sand-fixing, water and soil maintenance effects, 1 to 3 years old without pests and diseases, small head diameter ≥ 0.7cm robust growth Salix branches, in spring Carry out cuttage, cuttage adopts the configuration mode of plant row spacing 2m * 2m (about 222 plants/acre), all described Salix salix branches before cuttingtage use ABT rooting powder solution to carry out pretreatment, carry out before the end of June every year respectively within 2 years after cuttingtage One-time fertilization, the fertilization includes applying organic fertilizer poultry or livestock manure for all Salix salix, and simultaneously applying nitrogen, phosphorus, and potassium fertilizers. The amount of organic fertilizers is 1.5-3kg/plant; nitrogen, phosphorus, and potassium fertilizers are 0.2-0.3kg/plant .
所有植被种植区2内的沙柳成长后形成密度合理的植被层,植被层3用于 固定待治理沙漠区的地下土层即沙土层6,达到植物固定沙丘的效果。Salix salix in all vegetation planting areas 2 grows to form a vegetation layer with reasonable density, and the vegetation layer 3 is used to fix the underground soil layer in the desert area to be controlled, i.e. the sand layer 6, to achieve the effect of plant-fixed sand dunes.
所有的所述植被层3与所有的微生物覆膜层4共同作用固定所述待治理沙 漠区,如图2、3所示。All described vegetation layers 3 work together with all microbial coating layers 4 to fix the desert area to be controlled, as shown in Figures 2 and 3.
对经过上述沙漠原位微生物覆膜技术及其联合沙柳的绿色生态治理方法处 理过的沙漠覆膜层进行贯入仪测试;贯入仪检测微生物覆膜沙土层即微生物覆 膜层4的强度,为减小检测对覆膜效果造成的影响,检测过程从喷洒沙漠绿色 生物胶结剂的第四日开始,直到第七日结束。如图4所示分别为贯入试验贯入 阻力测试结果散点图和趋势图。The penetrometer test is carried out on the desert film layer treated by the above-mentioned desert in-situ microbial film technology and the green ecological management method combined with Salix salix; the penetrometer detects the strength of the microbial film sand layer, that is, the microbial film layer 4 , in order to reduce the impact of the detection on the film coating effect, the detection process starts from the fourth day of spraying the desert green bio-cement and ends on the seventh day. Figure 4 is the scatter diagram and trend diagram of the penetration resistance test results of the penetration test, respectively.
使用10cm长度贯入杆,检测时匀速下压贯入仪,记录贯入杆每厘米处贯 入仪的读数。贯入速度一般为1-1.5cm/s,贯入阻力不应小于17N。现场检测结 果显示,当在第五日对微生物覆膜沙土区进行检测时,所述微生物覆膜区的贯 入阻力达到17N,当在第七日对微生物覆膜区进行检测时,所述微生物覆膜沙 土区的贯入阻力达到47N。Use a penetrating rod with a length of 10 cm, press down on the penetrometer at a constant speed during detection, and record the readings of the penetrometer at each centimeter of the penetrating rod. The penetration speed is generally 1-1.5cm/s, and the penetration resistance should not be less than 17N. On-site test results show that when the microorganism-coated sandy soil area is tested on the fifth day, the penetration resistance of the microbial-coated area reaches 17N, and when the microbial-coated area is tested on the seventh day, the microbial The penetration resistance in the film-covered sandy soil area reaches 47N.
测试结果的判定:当微生物覆膜沙土层贯入阻力达到14-17N时即认为沙漠 原位微生物覆膜层的强度满足使用要求。Judgment of test results: When the penetration resistance of the microbial coating sand layer reaches 14-17N, it is considered that the strength of the in-situ microbial coating layer in the desert meets the requirements for use.
如图2、3所示,通过本发明提供的沙漠原位微生物覆膜技术及其联合沙柳 的绿色生态治理方法,在待治理沙漠区均匀喷洒可降解胶结颗粒,所述的胶结 颗粒具有高保水能力、所吸液体不被简单的物理方法挤出,并且可反复释液吸 液形成“微型水库”,提高沙漠生物覆膜层和植物种植区保液能力,改善沙漠覆 膜层材料脆性大的缺点;对待治理沙漠区分割为多个植被种植区和多个微生物 覆膜沙土区,所述微生物覆膜沙土区在沙漠自源微生物作用下形成原位微生物 固定层,形成的微生物覆膜沙土层可以从根本上阻断沙尘的源头,实现沙漠的 生态治理,另外,采用从待治理沙漠区的土壤中提取的沙漠自源微生物形成的 原位微生物覆膜层固定待治理沙漠区,可以做到以沙治沙,尽可能减少了外来 生物物种对待治理沙漠区造成的不良影响;所述植被种植区在沙柳作用下形成 植被层3,沙柳发达的根系5固定所述待治理沙漠区的地下土层即沙土层6,微 生物覆膜层4与植被层3相互依存、互为依托,共同构建沙漠生态系统的新格 局,二者结合,实现对待治理沙漠区的综合固定,同时,通过对原位微生物覆 膜层进行贯入仪测试,测试结果显示使用本发明所提出的方法,微生物覆膜层 4效果稳定,强度符合使用要求。As shown in Figures 2 and 3, through the desert in-situ microbial coating technology provided by the present invention and the green ecological treatment method combined with salix salix, the degradable cemented particles are evenly sprayed in the desert area to be treated, and the cemented particles have high Water retention capacity, the absorbed liquid is not squeezed out by simple physical methods, and can repeatedly release liquid and absorb liquid to form a "miniature reservoir", improve the liquid retention capacity of desert biological coatings and plant planting areas, and improve the brittleness of desert coating materials Disadvantages; the desert area to be treated is divided into multiple vegetation planting areas and multiple microbial-coated sandy soil areas. The layer can fundamentally block the source of sand and dust and realize the ecological management of the desert. In addition, the in-situ microbial coating layer formed by the desert's own source microorganisms extracted from the soil of the desert area to be treated is used to fix the desert area to be treated. Achieve sand control with sand, reduce the adverse effects of alien biological species on the treatment of desert areas as much as possible; the vegetation planting area forms a vegetation layer 3 under the action of Salix salix, and the developed root system 5 of Salix salix fixes the desert to be treated The underground soil layer in the area is the sand layer 6, the microbial coating layer 4 and the vegetation layer 3 are interdependent and rely on each other, and jointly build a new pattern of the desert ecosystem. The combination of the two realizes the comprehensive fixation of the desert area to be treated. Through the penetrometer test on the in-situ microbial coating layer, the test results show that using the method proposed in the present invention, the microbial coating layer 4 has a stable effect and its strength meets the requirements for use.
以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发 明的精神和原则之内所作的任何修改、等同替换和改进等,均应包含在本发明 的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.
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