CN108848732A - A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica - Google Patents

A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica Download PDF

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CN108848732A
CN108848732A CN201810725502.9A CN201810725502A CN108848732A CN 108848732 A CN108848732 A CN 108848732A CN 201810725502 A CN201810725502 A CN 201810725502A CN 108848732 A CN108848732 A CN 108848732A
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desert
microorganism
sandy soil
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李驰
斯日古楞
高瑜
王燕星
王硕
王翠艳
刘昌军
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Inner Mongolia University of Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • EFIXED CONSTRUCTIONS
    • E02HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02DFOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D3/00Improving or preserving soil or rock, e.g. preserving permafrost soil

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Abstract

The present invention discloses a kind of desert original position microorganism film coating technique and its combines the green ecological administering method of salix monogolica comprising step:It treats Desert Control area and carries out region segmentation, be segmented into multiple vegetation growing areas and multiple microorganism overlay film sandy soil areas to Desert Control differentiation for described;By desert from source bacterium CGMCC No.15633 seed activation, expand culture step by step;Desert is sprayed respectively in multiple microorganism overlay film sandy soil areas from source biology cementing agent, and each microorganism overlay film sandy soil area forms microorganism coating layer, the fixed surface layer sandy soil to Desert Control area under the action of desert is from source biology cementing agent;Plant more salix monogolicas respectively in the multiple vegetation growing area, each vegetation growing area forms vegetable layer, the fixed lower part sandy soil layer to Desert Control area in the case where corresponding salix monogolica acts on.The present invention can carry out overlay film in situ to desert soil, prevent invasive plants from suppressing and squeezing local biology, be truly realized with the purpose of husky sand control.

Description

A kind of desert original position microorganism film coating technique and its green ecological for combining salix monogolica are administered Method
Technical field
The present invention relates to a kind of green ecological administering method in desert more particularly to a kind of desert original position microorganism overlay films Technology and its green ecological administering method for combining salix monogolica.
Background technique
It desertifies caused by all parts of the world is because of windsand problem, erosion causes the reduction of biology and breaking for environment Bad, direct or indirect causes heavy economic losses and serious ecological problem to state and society.Therefore, various countries are in radix saposhnikoviae Field of fixing the sand all has put into a large amount of man power and material, it is therefore intended that therefore innovation control of desert mechanism also has received preferable Effect.It can not only be improved the ecological environment by the improvement to desert and agriculture-stock production condition, it is often more important that promoting to pass through Ji structural adjustment and social progress are also of great significance.
Aeolian Sandy Soil is the particularity soil developed in wind-blown sand sexupara matter, particle is tiny, psephicity is preferable, Intergranular is without cohesive strength, loose, easily flowing, easily wind erosion.There are many scholars to administer the control of desert desertification treatment both at home and abroad to ask Topic conducts in-depth research, and achieves preferable achievement.Such as, it is planted by research Atmospheric Nitrogen Deposition, microbe colony and desert By the mechanism that influences each other between three, the method for seeking to encourage desert vegetation finds that the settling amount of nitrogen increases, so that husky Nutrient increases in desert, microorganism enrichment, conducive to the growth of vegetation, according to the research of Desert Landscape conceputal modeling to biological soil Landforms control of earth skinning distribution etc..The patent application of publication number CN106717226A disclose a kind of application Stalk blanket into Row method for greening desert administers desert using Stalk blanket, there is grass seeds, tree species, the seeds of flowering plants, colza in Stalk blanket Deng, and mixed seeding is carried out, these seeds take root, germinate, and can improve the greening and ecology in desert.Publication number CN206118663U Patent application disclose a kind of sandy vegetation ecological management and its device, including annulus, be connected with and catchment on the outside of the annulus Blade, structure is simple, easy to operate, is made of degradation plastic, and the plastics after degradation can also supply nutrients for plant, dress It sets easy for installation, will not be blown away, husky body flowing amplitude can also maintain sandy land surface when little, do not destroy, be worth easily It must promote.Governance model is to carry out vegetative protection in the above invention, and the work of vegetative protection is embodied in the selection to plant species On, it is disadvantageous in that the package for fully relying on root system of plant, because desert climate condition makes green vegetation survive difficulty, i.e., Making to survive can only also make moving dunes become semifixed dune, fail the source that sand and dust are fundamentally truncated, while plant is solid The wind direction that sand will cause natural wind field changes, the wind field of disturbance the region that wind speed reduces occur sand and dust carry again with It accumulates again.
Using common control measures in chemical consolidation and desert engineer, the patent of 101548595 B of publication number CN Application discloses the desert control method of the chemical sand-fixing greening technology based on organic composite material, plants raw greening broadcasting sowing On the sand or soil layer of object, hydrophilic polyurethane resin curing agent solution is sprayed, Porous is formed and solidifies sand bed, do not enriching Underground water source, high wind erosion the peculiar environment in desert under, sand can be carried out stablizing, effective solidification, realize plant landscape function Energy and ecological recovery and improvement to Desertification Soil.The patent application of publication number CN106348872A discloses improvement and desertifies Soil remediation fertilizer of soil property and preparation method thereof, the soil remediation fertilizer using the improvement desertification soil property being prepared can have Effect increases soil fertility, reduces desertification degree.But soil is reinforced using the grouting of chemical building material, serious water and soil can be brought Pollution, causes adverse effect to environment.
Organic substance composite material is also a kind of sand prevention governing measure, and the patent application of publication number CN106358482A is public A kind of method for having opened Desert Control, sand ground, can by manpower or it is mechanical sprinkled water to desert, sandy land surface with random order and Solid organic materials are spread, or directly spill the solution with caking property and adhesiveness formed by solid organic materials, using stirring The solution of solid organic materials formation will be spilt or sprinkled with the desert after solid organic materials and watering, sandy land surface by mixing device Husky body is mixed thoroughly, keeps the sand on desert, sand ground surface layer modifies.But catabolite has certain toxic side effect, can give periphery ecology Bring harm.The patent application of publication number CN106947492A discloses a kind of composite sand consolidation model and preparation method thereof and answers With being sowed grass seeds by duster fiber filament by first ground on the scene, then spray bioreinforcement liquid on its surface, microorganism reinforces liquid in sandy soil table Layer and internal junction are hard, and reinforced structure is collectively formed in binding fiber silk, overcome original microorganism sand-fixation method surface brittleness it is big, Durability is poor, easily wind erosion the shortcomings that, but its bioreinforcement liquid used is external strain, and growth is difficult to control, and destroys ecosystem The structure and engineering of system lead to original biocenological decline and disappearance, and irreversible pollution may be caused to environment.
Summary of the invention
In order to solve the problems in the existing technology, the present invention propose a kind of desert original position microorganism film coating technique and Its green ecological administering method for combining salix monogolica., from source strain, desert soil is carried out in situ with screened from area desert to be administered Overlay film prevents invasive plants from suppressing and squeezing local biology, is truly realized with the purpose of husky sand control.In this technical foundation Drought-enduring, weather-proof, the resistance to sand of upper joint buries, grow rapidly, rhizome is flourishing, checks winds and fixes drifting sand, the plant for the obvious effect that conserves water and soil sand Sandy soil under willow fixation of microbe coating layer provide good green for control of desert and administer linkage structure.
The present invention is implemented with the following technical solutions:A kind of desert original position microorganism film coating technique and its joint salix monogolica Green ecological administering method comprising step:
Step 1:It treats Desert Control area and carries out region segmentation, be segmented into multiple vegetation kinds to Desert Control differentiation for described Growing area and multiple microorganism overlay film sandy soil areas;
Step 2:By desert from source bacterium CGMCC No.15633 seed activation, expand culture step by step;
Step 3:On the basis of step 1, desert Zi Yuan is sprayed respectively in multiple microorganism overlay film sandy soil areas Biological cementing agent, each microorganism overlay film sandy soil area form microorganism coating layer under the action of desert is from source biology cementing agent, The fixed surface layer sandy soil to Desert Control area;
The desert from source biology cementing agent include desert from source bacterium solution, cementing nutrient solution and cement particle;The sand Desert is passed through by the desert of step 2 from source bacterium from source bacterium solution to spread cultivation;The cementing nutrient solution is by CO (NH2)2、 CaCl2.2H2O material composition;The cement particle main component is degradable macromolecule water absorbent polymer;
Step 4:Plant more salix monogolicas respectively in the multiple vegetation growing area, each vegetation growing area is corresponding Vegetable layer, the fixed lower part sandy soil layer to Desert Control area are formed under salix monogolica effect.
As a further improvement of the foregoing solution, multiple microorganism overlay film sandy soil areas and multiple vegetation are planted The interlaced distribution in area.
Further, multiple microorganism overlay film sandy soil area's array layouts, the four of each microorganism overlay film sandy soil area Angle is laid out the vegetation growing area respectively.
As a further improvement of the foregoing solution, the cement particle is uniformly sprayed into described with every square metre 50-70 grams In microorganism overlay film sandy soil area;One square metre of microorganism overlay film sandy soil area sprinkling 5-6 rises desert from source bacterium solution, 12-15 The cementing nutrient solution risen.
As a further improvement of the foregoing solution, with the latter day of cement particle sprinkling day for first day, described First, the desert was from source bacterium solution and the cementing nutrient solution with volume ratio for 1:2.5 ratio is alternately sprayed, Alternating sequence is first to spray the desert from source bacterium solution, then spray the cementing nutrient solution, successively alternately;The desert The interval time for replacing sprinkling with the cementing nutrient solution from source bacterium solution is 1 hour;Second and third, daily respectively sprinkling on the four with The cementing nutrient solution of same volume on the firstth.
Further, desert is sprayed in 4 hours of described first day altogether from source bacterium solution and cementing nutrient solution each 2 times.
Further, the sprinkling process of the cement particle, the desert from source bacterium solution and the cementing nutrient solution this two The process for preparation and sprinkling process of kind liquid, are respectively completed each by different sprinkling irrigation equipments.
As a further improvement of the foregoing solution, in step 2:Desert is living from source bacterium CGMCC No.15633 seed Change, then accesses shaking flask, seeding tank and fermentor and expand culture step by step.
As a further improvement of the foregoing solution, the desert is passed through from source bacterium by the desert from source bacterium solution and is spread cultivation simultaneously Fresh culture composition is added after centrifugation, the fresh culture is by 20 grams/every liter of yeast powder, 0.1~1molNaCl, 0.05 ~1molNH4Cl, 0.1~1molCO (NH2)2Material composition.
As a further improvement of the foregoing solution, the cementing nutrient solution is by 0.4mol~1molCO (NH2)2、 0.25mol ~0.5molCaCl2.2H2O material composition.
The advantage of the invention is that:
1, using the desert screened from the soil to Desert Control area, from source microorganism, (i.e. desert is from source bacterium CGMCC No.15633) the fixed surface layer to Desert Control area, the microorganism overlay film that forming properties are stable, intensity is high, water-retaining property is strong, from The source of sand and dust is fundamentally blocked, while from the use of source microorganism reducing external microorganism and treats Desert Control area and causing Influence, realize application of the microorganism film coating technique in situ of control of desert in the green ecological management of desert;
2, it adds degradable cement particle from the fixed surface layer of desert sandy soil of source microorganism using desert and is formed by micro- life Object coating layer has good water-permeable and air permeable performance, atmosphere vapour or rainwater etc. can be penetrated into lower part sandy soil layer, for plantation Area's root system of plant provides enough aqueous vapors and nourishes, so that the survival rate of vegetation is improved significantly;
3, with to the fixed surface layer of desert sandy soil of the microorganism in Desert Control area, the fixed desert bottom sandy soil of vegetation root system, two It is on the basis of not destroying original sand ground ecology self equilibrium systems, by green micro organism technology and desert ecological that person, which combines, The perfect combination of environmental improvement is that the green of desert ecological system is administered and the original scientific and technical innovation in recovery.
Detailed description of the invention
Fig. 1 is to treat Desert Control area to carry out schematic diagram after region segmentation;
Fig. 2 is desert original position microorganism film coating technique and its green ecological Desert Control regulation effect figure for combining salix monogolica;
Fig. 3 is the fixed surface layer of desert soil of microorganism coating layer, the fixed desert underearth structural schematic diagram of plant;
Fig. 4 is using method provided by the invention to the fixed sandy soil layer penetration resistance test result tendency chart of microorganism.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, The present invention will be described in further detail.It should be appreciated that described herein, specific examples are only used to explain the present invention, It is not intended to limit the present invention.
Embodiment 1
The desert original position microorganism film coating technique of the present embodiment and its green ecological administering method for combining salix monogolica include with Lower step.
Step 1:It treats Desert Control area and carries out region segmentation, be segmented into multiple vegetation kinds to Desert Control differentiation for described Growing area and multiple microorganism overlay film sandy soil areas.
Multiple microorganism overlay film sandy soil areas are preferably mutually interspersed with multiple vegetation growing areas, preferably It is laid out for multiple microorganism overlay film sandy soil area's arrays, the quadrangle in each microorganism overlay film sandy soil area is laid out one respectively The vegetation growing area.In addition, multiple microorganism overlay film sandy soil areas and multiple interlaced point of vegetation growing areas Cloth can also meet the needs of local Desert Landscape design.
Step 2:By desert from source bacterium CGMCC No.15633 seed activation, expand culture step by step.
In the present embodiment, by desert from source bacterium CGMCC No.15633 seed activation, then access shaking flask, seeding tank and Fermentor expands culture step by step.The bacterium is to prevent from source microorganism from the extraction of source microorganism with cementation solidifying effect Flora, which is rapidly bred, behind adventive intrusion suitable growth new district leads to destroy local ecological safety.Bacterial strain gy1#, in On April 18th, 2018 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as CGMCC), Collection registration number is CGMCC No.15633.The bacterium is staphylococcus Staphylococcus sp..
Step 3:On the basis of step 1, desert Zi Yuan is sprayed respectively in multiple microorganism overlay film sandy soil areas Biological cementing agent, each microorganism overlay film sandy soil area form microorganism coating layer under the action of desert is from source biology cementing agent, The fixed surface layer sandy soil to Desert Control area.
The desert from source biology cementing agent include desert from source bacterium solution, cementing nutrient solution and cement particle.The sand Desert is passed through by the desert of step 2 from source bacterium from source bacterium solution to spread cultivation, and such as the desert is from source bacterium solution by the desert Zi Yuan Bacterium is added fresh culture composition after spreading cultivation and being centrifuged, the fresh culture by 20 grams/every liter of yeast powder, 0.1~ 1molNaCl, 0.05~1molNH4Cl, 0.1~1molCO (NH2)2Material composition.;The cementing nutrient solution is by CO (NH2)2、 CaCl2.2H2O material composition, such as cementing nutrient solution is by 0.4mol~1molCO (NH2)2, 0.25mol~ 0.5molCaCl2.2H2O material composition.The cement particle main component is degradable macromolecule water absorbent polymer, is uniformly sprayed It is spilled into the soil in microorganism control of desert area.
With the latter day of cement particle sprinkling day for first day, at described first day, from source bacterium solution and cementing nutrition Liquid is with volume ratio for 1:2.5 ratio is alternately sprayed, and specific alternating sequence is first to spray from source bacterium solution, then spray cementing Nutrient solution, successively alternately, the interval time for replacing sprinkling with cementing nutrient solution from source bacterium solution are 1 hour.The Two, the cementing nutrient solution of daily respectively sprinkling and same volume on the firstth on the three, four.The sprinkling of the cement particle and two kinds The process for preparation of consolidating fluid is respectively completed from sprinkling process each by different sprinkling irrigation equipments.
In implementation process, the cement particle is uniformly sprayed into the microorganism overlay film sand with every square metre 50-70 grams In native area;One square metre of microorganism overlay film sandy soil area sprinkling 5-6 rises desert from source bacterium solution, and 12-15 rises described cementing Nutrient solution.With the latter day of cement particle sprinkling day for first day, at described first day, the desert from source bacterium solution and The cementing nutrient solution is with volume ratio for 1:2.5 ratio is alternately sprayed, and alternating sequence is first to spray the desert certainly Source bacterium solution, then the cementing nutrient solution is sprayed, successively alternately;It is handed over from source bacterium solution and the cementing nutrient solution in the desert The interval time that tubing flow displacement is spilt is 1 hour;Second and third, the cementing nutrition of daily respectively sprinkling and same volume on the firstth on the four Liquid.Desert is sprayed in 4 hours of described first day altogether from source bacterium solution and cementing nutrient solution each 2 times.The sprinkling of the cement particle Process, the desert is from the process for preparation of source bacterium solution and cementing both liquid of nutrient solution and sprays process, each by Different sprinkling irrigation equipments are respectively completed.
The thickness of microorganism coating layer can be adjusted according to environment and the target of improvement.
Step 4:Plant more salix monogolicas respectively in the multiple vegetation growing area, each vegetation growing area is corresponding Vegetable layer, the fixed lower part sandy soil layer to Desert Control area are formed under salix monogolica effect.
Salix monogolica is that minority can not be afraid of husky pressure in one of salt-soda soil growing plants, branch, and rudiment power is strong, well developed root system, Do not destroyed while being fixed sandy soil, do not pollute the material property of sandy soil itself, but be capable of fixing sandslide, stablize it is water-based Preferred plant.As for the density of vegetable layer, can be adjusted according to environment and the target of improvement.
A kind of desert original position microorganism film coating technique that there is provided through the invention and its green ecological for combining salix monogolica are administered Method treats Desert Control differentiation and is segmented into multiple vegetation growing areas and multiple microorganism overlay film sandy soil areas, the microorganism overlay film Sandy soil area forms microorganism coating layer under the action of desert is from source biology cementing agent, and the surface layer in fixed Desert Control area of institute is husky Soil, the vegetation growing area form vegetable layer in the case where salix monogolica acts on, the fixed lower part sandy soil layer to Desert Control area, and two Person combines, and realizes the comprehensive treatment for treating Desert Control area.
Embodiment 2
Part microorganism can induce during metabolism and generate calcium carbonate in nature, can be by loose sand It is cemented to some strength, the good green novel artificial material of permeability.
Since desert soil is the good carrier of microorganism, can be grown in porous media using microorganism, migrate and The characteristics such as breeding separate in desert with fixed, bond effect from source strain, generate desert microorganism coating layer fixation table Layer sandy soil fundamentally block the source of sandstorm so that moving dunes become semifixed, fixed dune through fixed overlay film. Microorganism coating layer shows the features such as with ground matrix compatibility, good ventilative water permeability, water-retaining property, it is allowed to become sand In unconcerned ecological management " natural barrier that can be breathed ".Further, it selects drought-enduring, impoverishment tolerant planting and realizes that its optimization is matched It sets, realizes interdependence between microorganism coating layer in situ and sand ground green plants, relies on each other, and construct unconcerned life of rustling jointly The new frame of state system.
Based on the above analysis, the present invention provides a kind of desert original position microorganism film coating technique and its combines the green of salix monogolica Ecological treatment method includes the following steps.
Step 1:It treats Desert Control area and carries out region segmentation, be segmented into multiple vegetation kinds to Desert Control differentiation for described Growing area 2 and multiple microorganism overlay film sandy soil areas 1.
As shown in Figure 1, multiple microorganism overlay film sandy soil areas 1 and multiple vegetation growing areas 2 intermesh point Cloth.Multiple microorganism overlay film sandy soil area's arrays are laid out, and are every length and width in the region that length and width are ten thousand metres Multiple microorganism overlay film sandy soil areas 1 of 2000 meters of setting array, the quadrangle difference in each microorganism overlay film sandy soil area 1 It is laid out a vegetation growing area 2, the diameter of the vegetation growing area 2 is 300 meters.
Step 2:Shaking flask is accessed and tank expands step by step from source bacterium (CGMCC No.15633) seed activation in the desert Culture.
Step 3:To desert is sprayed in all microorganism overlay film areas 1 from source green bio cementing agent, formed certain The microorganism coating layer 4 of thickness, the desert from source biology cementing agent include desert from source bacterium solution, cementing nutrient solution and Cement particle.
Degradable cement particle is sprayed in microorganism overlay film area 1, to make the fixed sandy soil area fixing layer tool of microorganism There are good strength, plasticity and permeability, cement particle is uniformly sprayed into every square metre 50-70 grams and described needs to be controlled In the soil for managing microorganism overlay film sandy soil area, the main component of the cement particle is degradable macromolecule water absorbent polymer.
With the latter day of water-retaining agent, that is, cement particle sprinkling day for first day, described first day, from source bacterium solution and Cementing nutrient solution is with volume ratio for 1:2.5 ratio is alternately sprayed, specially one square metre of microorganism curing area sprinkling The cementing nutrient solution risen from source bacterium solution, 12-15 that 5-6 rises, alternating sequence are first to spray from source bacterium solution, then spray cementing nutrition Liquid, successively alternately, the interval time for replacing sprinkling with cementing nutrient solution from source bacterium solution are 1 hour, i.e., from source bacterium solution Cementing nutrient solution is sprayed after having sprayed one hour, then is sprayed after an hour from source bacterium solution, then sprays cementing nutrition after an hour Liquid sprays in described 4 hours first day altogether from source bacterium solution and cementing nutrient solution each 2 times.Second and third, daily respectively sprinkling on the four With the cementing nutrient solution of same volume on the firstth.The cement particle, process for preparation and spray from source bacterium solution and cementing nutrient solution Process is spilt to be respectively completed each by different sprinkling irrigation equipments.
It is described fresh culture is added after spreading cultivation and being centrifuged from source microorganism by desert from source bacterium solution to form, institute Fresh culture is stated by 20 grams/every liter of yeast powder, 0.1~1molNaCl, 0.05~1molNH4Cl, 0.1~1molCO (NH2)2 Material composition.The cementing nutrient solution is by 0.4mol~1molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O substance Composition.
Step 4:Salix monogolica is planted respectively in all vegetation growing areas 2, is respectively formed the vegetable layer of certain density 3.It is that all salix monogolicas apply base fertilizer according to salix monogolica kind and quantity, supplies required nutrient in the entire growth cycle of salix monogolica.
The salix monogolica kind choose drought-enduring, weather-proof, resistance to sand bury, grow rapidly, check winds and fix drifting sand, the effect that conserves water and soil is shown The salix monogolica branch of work, 1~triennial no disease and pests harm, top diameter >=0.7cm robust growth carries out cuttage, cuttage in spring Using the configuration mode of seeding row spacing 2m × 2m (about 222 plants/acre), all salix monogolica branches are molten using ABT root-inducing powder before cuttage Liquid is pre-processed, and carries out single-dose application in 2 after cuttage year before by the end of June every year respectively, the fertilising is including being all Salix monogolica organic fertilizer fowl or animal manure just, while cooperating and applying nitrogen, phosphorus, potash fertilizer, and organic manure application rate is 1.5~3kg/ plants;Nitrogen, phosphorus, 0.2~0.3kg/ plants of potash fertilizer.
Form the reasonable vegetable layer of density after salix monogolica growth in all vegetation growing areas 2, vegetable layer 3 for it is fixed to Sub-surface, that is, the sandy soil layer 6 in Desert Control area, achievees the effect that plant fixed dune.
All vegetable layer 3 and all 4 collective effects of microorganism coating layer are fixed described to Desert Control area, As shown in Figure 2,3.
It is processed to the green ecological administering method by above-mentioned desert original position microorganism film coating technique and its joint salix monogolica Desert coating layer carry out penetrometer test;Penetrometer detects microorganism overlay film sandy soil layer, that is, microorganism coating layer 4 intensity, Influenced caused by overlay film effect to reduce detection, detection process since sprinkling the 4th day of desert green bio cementing agent, Until the 7th end of day.It is as shown in Figure 4 respectively penetration test penetration resistance test result scatter plot and tendency chart.
Using 10cm length injection bar, when detection, at the uniform velocity pushes penetrometer, records the reading of injection bar place's penetrometer per cm Number.Penetrating speed is generally 1-1.5cm/s, and penetration resistance is no less than 17N.On-site test is the results show that when right on 5th When microorganism overlay film sandy soil area is detected, the penetration resistance in microorganism overlay film area reaches 17N, when on 7th to micro- When biological overlay film area is detected, the penetration resistance in the microorganism overlay film sandy soil area reaches 47N.
The judgement of test result:Desert original position is thought when microorganism overlay film sandy soil layer penetration resistance reaches 14-17N The intensity of microorganism coating layer meets requirement.
As shown in Figure 2,3, the green of the desert original position microorganism film coating technique and its joint salix monogolica that provide through the invention Ecological treatment method is uniformly spraying degradable cement particle to Desert Control area, and the cement particle has high water conservation energy Power, institute's imbibition body are not squeezed out by simple physical method, and can be released liquid imbibition repeatedly and be formed " miniature reservoir ", and desert is improved Biological coating layer and plant species growing area liquid-keeping property improve the big disadvantage of desert coating layer material fragility;Treat Desert Control area Multiple vegetation growing areas and multiple microorganism overlay film sandy soil areas are divided into, the microorganism overlay film sandy soil area is micro- from source in desert Microorganism fixing layer in situ is formed under biological effect, the microorganism overlay film sandy soil layer of formation can fundamentally block sand and dust The ecological management in desert is realized in source, in addition, using the desert extracted from the soil to Desert Control area from source microorganism The microorganism coating layer in situ of formation is fixed to Desert Control area, can accomplish to reduce outer next life as far as possible with husky sand control Object species treat adverse effect caused by Desert Control area;The vegetation growing area forms vegetable layer 3 in the case where salix monogolica acts on, husky Fixed sub-surface, that is, the sandy soil layer 6 to Desert Control area of the root system 5 of Liu Fada, microorganism coating layer 4 and vegetable layer 3 Interdependence relies on each other, and the common new frame for constructing desert ecological system, the two combines, and Desert Control area is treated in realization It is comprehensive to fix, meanwhile, by carrying out penetrometer test to microorganism coating layer in situ, test result display uses institute of the present invention The method of proposition, 4 effect stability of microorganism coating layer, intensity meet requirement.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.

Claims (10)

1. a kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica, which is characterized in that its Including step:
Step 1:It treats Desert Control area and carries out region segmentation, be segmented into multiple vegetation growing areas to Desert Control differentiation for described With multiple microorganism overlay film sandy soil areas;
Step 2:By desert from source bacterium CGMCC No.15633 seed activation, expand culture step by step;
Step 3:On the basis of step 1, desert is sprayed respectively in multiple microorganism overlay film sandy soil areas from source biology Cementing agent, each microorganism overlay film sandy soil area form microorganism coating layer under the action of desert is from source biology cementing agent, fixed The surface layer sandy soil to Desert Control area;
The desert from source biology cementing agent include desert from source bacterium solution, cementing nutrient solution and cement particle;The desert is certainly Source bacterium solution is by the desert of step 2 from source bacterium by spreading cultivation;The cementing nutrient solution is by CO (NH2)2、CaCl2.2H2O substance Composition;The cement particle main component is degradable macromolecule water absorbent polymer;
Step 4:Plant more salix monogolicas respectively in the multiple vegetation growing area, each vegetation growing area is in corresponding salix monogolica Vegetable layer, the fixed lower part sandy soil layer to Desert Control area are formed under effect.
2. desert original position microorganism film coating technique as described in claim 1 and its green ecological administering method for combining salix monogolica, It is characterized in that, multiple microorganism overlay film sandy soil areas and multiple interlaced distributions of vegetation growing area.
3. desert original position microorganism film coating technique as claimed in claim 2 and its green ecological administering method for combining salix monogolica, It is characterized in that, multiple microorganism overlay film sandy soil area's array layouts, the quadrangle difference in each microorganism overlay film sandy soil area It is laid out a vegetation growing area.
4. desert original position microorganism film coating technique as described in claim 1 and its green ecological administering method for combining salix monogolica, It is characterized in that, the cement particle is uniformly sprayed into the microorganism overlay film sandy soil area with every square metre 50-70 grams;One is flat The microorganism overlay film sandy soil area sprinkling 5-6 of square rice rises the cementing nutrient solution that desert rises from source bacterium solution, 12-15.
5. desert original position microorganism film coating technique as described in claim 1 or 4 and its green ecological improvement side for combining salix monogolica Method, which is characterized in that with the latter day of cement particle sprinkling day for first day, at described first day, the desert Zi Yuan Bacterium solution and the cementing nutrient solution are with volume ratio for 1:2.5 ratio is alternately sprayed, and alternating sequence is described in first sprinkling Desert is from source bacterium solution, then sprays the cementing nutrient solution, successively alternately;The desert is from source bacterium solution and the cementing nutrition The interval time that liquid alternately sprays is 1 hour;Second and third, the cementing battalion of daily respectively sprinkling and same volume on the firstth on the four Nutrient solution.
6. desert original position microorganism film coating technique as claimed in claim 5 and its green ecological administering method for combining salix monogolica, It is characterized in that, desert is sprayed in 4 hours of described first day altogether from source bacterium solution and cementing nutrient solution each 2 times.
7. desert original position microorganism film coating technique as claimed in claim 5 and its green ecological administering method for combining salix monogolica, It is characterized in that, the sprinkling process of the cement particle, the desert is from source bacterium solution and described both liquid of cementing nutrient solution Process for preparation and sprinkling process, be respectively completed each by different sprinkling irrigation equipments.
8. desert original position microorganism film coating technique as described in claim 1 and its green ecological administering method for combining salix monogolica, It is characterized in that, in step 2:By desert from source bacterium CGMCC No.15633 seed activation, then access shaking flask, seeding tank and Fermentor expands culture step by step.
9. desert original position microorganism film coating technique as described in claim 1 and its green ecological administering method for combining salix monogolica, It is characterized in that, fresh culture group is added from source bacterium by the desert from source bacterium solution in the desert after spreading cultivation and being centrifuged At the fresh culture is by 20 grams/every liter of yeast powder, 0.1~1molNaCl, 0.05~1molNH4Cl, 0.1~1molCO (NH2)2Material composition.
10. desert original position microorganism film coating technique as described in claim 1 and its green ecological administering method for combining salix monogolica, It is characterized in that, the cementing nutrient solution is by 0.4mol~1molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O substance Composition.
CN201810725502.9A 2018-07-04 2018-07-04 A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica Pending CN108848732A (en)

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Application publication date: 20181123