CN109518703B - Microbial mineralization and cementation treatment fluid and treatment method of sandy slope - Google Patents
Microbial mineralization and cementation treatment fluid and treatment method of sandy slope Download PDFInfo
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- CN109518703B CN109518703B CN201910037592.7A CN201910037592A CN109518703B CN 109518703 B CN109518703 B CN 109518703B CN 201910037592 A CN201910037592 A CN 201910037592A CN 109518703 B CN109518703 B CN 109518703B
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D17/00—Excavations; Bordering of excavations; Making embankments
- E02D17/20—Securing of slopes or inclines
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a microbial mineralization cementation treatment fluid and a treatment method of a sandy slope. The microbial mineralization cementation treatment liquid consists of mineralized bacterial liquid, bacillus liquid and calcium source mixed solution which are independent from each other and have the volume ratio of 1 (5-10) to (1-3). The mineralized bacterial liquid is divided into 1# pasteurella bacillus liquid and 2# staphylococcus liquid; the 1# Pasteur bacillus liquid is suitable for treating sandy soil with slope rate less than 1:1 and particle size range d less than 20 mm; the 2# staphylococcus liquid is suitable for treating sandy soil with slope rate less than 1:1 and grain size range of 0.074mm < d < 10 mm. When the invention is used for treating the sandy side slope, firstly, mineralized bacterial liquid is sprayed on the sandy side slope, then, calcium source mixed solution is sprayed, and the mineralized bacterial liquid and the calcium source mixed solution are sprayed twice each day in the sequence, and the mineralized bacterial liquid and the calcium source mixed solution are continuously sprayed for five days until a solidified layer on the sandy side slope reaches a certain thickness, and then, the bacillus bacterial liquid is sprayed.
Description
Technical Field
The invention relates to a green and environment-friendly side slope protection method in the technical field of highway side slope protection, in particular to a microbial mineralization cementing treatment fluid for treating sandy side slopes which are easy to erode and a treatment method for sandy side slopes which are easy to erode by adopting the microbial mineralization cementing treatment fluid.
Background
With the rapid development of economy in China, the construction projects of roads are gradually increased, the construction requirements are higher and higher, but the landslide of the side slope of the roadbed is one of the common diseases of the roadbed of the road, traffic is blocked, the driving safety is endangered, the stability of the side slope has great influence on the overall performance of the roadbed structure, and serious economic loss is brought to local residents. For many sandy slopes, the cohesive force is small, the stability is poor, the sandy slopes are easily washed by rainwater, so that water and soil loss is caused, geological disasters such as collapse and landslide occur, the environment is seriously damaged, and safety accidents are easily caused.
At present, conventional protective measures, namely geosynthetic material protection, asphalt protection, concrete frame, grass planting and other protective technologies are mainly adopted to enhance the overall stability and the scouring resistance of the side slope soil body, but the conventional protective measures have many defects, and for northern cold and arid regions, the conventional protective measures have the disadvantages of less dryness, less rain, more wind and sand, sparse vegetation, strong ground surface erosion and cutting, low grass planting survival rate and extremely poor growth condition, are difficult to maintain the stability of a roadbed, and cannot achieve good protective effects.
Disclosure of Invention
Aiming at the situation, the invention provides a microbial mineralization cementation treatment fluid for treating sandy side slope easy to erode and a method for treating sandy side slope easy to erode by using the microbial mineralization cementation treatment fluid. The method for treating sandy side slope of the invention belongs to effective microorganism side slope ecological protection, which is not only beneficial to beautifying the environment, but also can effectively prevent water and soil loss of the side slope, and plays a role in protecting and stabilizing highway subgrade.
The invention is realized by adopting the following technical scheme: a microorganism mineralized cementation treatment fluid for treating sandy side slopes which are easy to erode consists of mineralized bacterium liquid, bacillus liquid and calcium source mixed solution;
the mineralized bacterium liquid has mineralization capacity and is divided into 1# pasteurella bacillus liquid and 2# staphylococcus liquid: the OD600 value of the 1# Pasteurella barnacii liquid is 0.6-0.8; the OD600 value of the 2# staphylococcus bacterium liquid is 1.0-2.0;
the bacillus liquid has cementing and cohering capacity, and the OD600 value is 1.0-2.0;
the calcium source mixed solution comprises a calcium source and nutrients, and the calcium ion concentration of the calcium source is between 0.25mol/L and 1 mol/L;
wherein the mineralized bacteria liquid, the bacillus liquid and the calcium source mixed solution are mutually independent, and the mineralized bacteria liquid, the bacillus liquid and the calcium source mixed solution areThe spraying volume ratio of (1), (5-10) to (1-3); when the microbial mineralization cementation treatment liquid is used, the spraying amount of the mineralized bacterium liquid is 300-400L/m3Firstly spraying the mineralized bacteria liquid on a sandy slope, then spraying the calcium source mixed solution on the sandy slope sprayed with the mineralized bacteria liquid, and spraying the mineralized bacteria liquid and the calcium source mixed solution twice every day in this order, wherein the spraying time interval of the mineralized bacteria liquid and the calcium source mixed solution is 0.5-1 h every time, and the bacillus liquid is sprayed after the spraying time interval is continuously five days until a solidified layer on the sandy slope reaches a certain thickness.
As a further improvement of the scheme, the mineralized bacterial strain adopted by the mineralized bacterial liquid is domesticated and cultured in the culture medium for 3-4 times, the mineralized bacterial strain is inoculated into the culture medium according to the inoculation amount of 1% -5% every time, the culture temperature is 30-40 ℃, the culture temperature is 180-200 r/min, the pH value is 6.0-6.5, and the culture time is 2 d-4 d.
As a further improvement of the scheme, the culture medium of the 1# pasteurella bacillus liquid comprises yeast powder, (NH)4)2SO40.13M tris buffer solution and deionized water, wherein in each liter of deionized water, the yeast powder accounts for 20g/L, (NH)4)2SO410g/L of tris buffer, and 15.748g/L of 0.13M tris buffer.
As a further improvement of the scheme, the 2# staphylococcus bacteria liquid culture medium comprises 12-18 g/L of yeast powder and 0.05-0.1 mol/L of NH4Cl, 0.1-0.5 mol/L CO (NH)2)2And (4) forming.
As a further improvement of the above scheme, the calcium source is selected from calcium nitrate, calcium chloride and calcium acetate.
As a further improvement of the scheme, the nutrient comprises 0.2 mol/L-0.35 mol/L NH4Cl, 3% -5% NutrientBroth and 0.5-0.8 mol/L CO (NH)2)20.1 mol/L-0.2 mol/L NaHCO3。
As a further improvement of the scheme, the calcium source mixed solution dissolves the calcium source and the water of the nutrients, and the pH value of the calcium source mixed solution is between 6 and 7.
The invention also provides a treatment method of the sandy slope which is easy to erode, which comprises the following steps:
providing any one of the microbial mineralization cementing treatment fluid for treating sandy slopes;
firstly, spraying a mineralized bacterium liquid of the microbial mineralization cementation treatment liquid on a sandy slope;
spraying a calcium source mixed solution of the microorganism mineralization cementation treatment fluid on the sandy slope sprayed with the mineralized bacterium liquid;
spraying the mineralized bacterium liquid and the calcium source mixed solution twice every day in this sequence, wherein the spraying time interval of the mineralized bacterium liquid and the calcium source mixed solution is 0.5-1 h each time, and continuously spraying for 5 days until a solidified layer on a sandy slope reaches a certain thickness, and then spraying the bacillus liquid of the microorganism mineralized cementation treatment liquid;
wherein the spraying amount of the mineralized bacterium liquid is 300-400L/m3The mineralized bacterium liquid is the bacillus liquid, and the calcium source mixed solution is sprayed in a volume ratio of 1 (5-10) to 1-3.
As a further improvement of the above aspect, the thickness of the cured layer is 5cm to 10 cm.
As a further improvement of the scheme, the method for protecting the 1# pasteurella bacillus liquid aims at sandy soil with a slope rate of less than 1:1 and a particle size range d of less than 20 mm;
the 2# staphylococcus liquid treatment method is suitable for sandy soil with a slope gradient of less than 1:1 and a grain size range of 0.074mm and d of less than 10 mm.
The invention has the advantages that:
(1) the bacillus pasteurii, the staphylococcus and the bacillus are all soil-derived bacteria, so that the original ecological environment of soil is not damaged;
(2) the bacillus liquid with cementation capability is added, so that the brittleness of the soil solidified by the traditional technology is further improved, the cementation capability among soil particles is improved, and the stability of the sandy slope is enhanced; the loose sandy soil particles are consolidated under the combined action of mineralization and cementation, so that the sandy soil side slope can be prevented from being eroded and damaged;
(3) the traditional slope curing method is changed, the spraying method is simple to operate, the construction period is short, the manufacturing cost is low, the effect is obvious, the process is realized, the ecological environment protection concept is met, and the application prospect is wide.
Preservation of biological materials
The Bacillus strain provided by the invention is GiLi 1#, the name of the microbe is Bacillus sp, the Bacillus strain is preserved in China general microbiological culture Collection center (CGMCC for short, the address: China academy of sciences, 3, North West Lu No.1 institute of China, North Kogyo, Chaoyang, Beijing) in 27.7.8.7.8.3.A preservation center registration number is CGMCC No. 16169.
Drawings
FIG. 1 is a flow chart of a method for treating a sandy slope which is easy to erode and is made of sand;
FIG. 2 is a diagram of a sandy slope selected in the test;
FIG. 3 is a graph of mineralized bacteria used in the experiment;
FIG. 4 is a microscopic image of the slope after mineralization in the experiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The invention relates to a method for treating a sandy slope which is easy to erode, and please refer to fig. 1, which comprises the following steps:
providing a microorganism mineralized and cemented treatment fluid for treating sandy slopes;
firstly, spraying a mineralized bacterium liquid of the microbial mineralization cementation treatment liquid on a sandy slope;
spraying a calcium source mixed solution of the microorganism mineralization cementation treatment fluid on the sandy slope sprayed with the mineralized bacterium liquid;
spraying the mineralized bacterium liquid and the calcium source mixed solution twice every day in this sequence, wherein the spraying time interval of the mineralized bacterium liquid and the calcium source mixed solution is 0.5-1 h each time, continuously spraying for five days until a solidified layer on a sandy slope reaches a certain thickness (such as 5 cm-10 cm), and then spraying the bacillus liquid of the microbial mineralization cementing treatment liquid;
wherein the spraying amount of the mineralized bacterium liquid is 300-400L/m3The spraying volume ratio of the mineralized bacterium liquid to the bacillus liquid to the calcium source mixed solution is1 (5-10) to (1-3).
The sandy side slope selected in the test is shown in figure 2, the particles are loose and slide and are easy to erode, and the sandy soil with the side slope rate less than 1:1 and the particle size range of 0.074mm less than d less than 10mm is difficult to treat by adopting the traditional method. The invention is suitable for sandy soil with slope rate less than 1:1 and grain size range 0.074mm < d < 10 mm.
The microbial mineralization cementation treatment fluid is different from a common treatment fluid, and firstly, the cementation capability and the mineralization capability are very strong, the manufacturing cost is low, and the construction period is short. And secondly, the microorganism mineralization and cementation treatment liquid consists of mineralized bacterium liquid, bacillus liquid and calcium source mixed solution which are mutually independent. This is one of the key points of the present invention.
When the calcium source mixed solution is used, the spraying volume ratio of the mineralized bacteria liquid to the bacillus liquid to the calcium source mixed solution is1 (5-10) to (1-3), and the spraying amount of the mineralized bacteria liquid is 300-400L/m3. The respective concentrations satisfy the following conditions:
the staphylococcus bacteria liquid has mineralization capability, and the OD600 value is 1.0-2.0;
the bacillus liquid has cementing and cohering capacity, and the OD600 value is 1.0-2.0;
the calcium source mixed solution comprises a calcium source and nutrients, and the calcium ion concentration of the calcium source is between 0.25mol/L and 1 mol/L.
The staphylococcus strains are domesticated and cultured in a culture medium for 3-4 times, the staphylococcus strains are inoculated into the culture medium according to 1-5% of inoculation amount each time, the inoculation time is 24-48 hours each time, and the culture temperature is 30-40 DEG C180-200 r/min, pH6.0-6.5, and the culture time is 2-4 d. In this embodiment, as shown in fig. 3, the staphylococcus mineralized bacteria strain is gy1#, is a known strain, and has been deposited in the general microbiological center of the china microbiological culture collection administration (CGMCC, address: china academy of sciences institute of china, No.1, west way, No. 3, north chen, south china, north, beijing) on 18/4/2018, and the registration number of the deposition center is CGMCC No. 15633. The culture medium comprises 12-18 g/L yeast powder and 0.05-0.1 mol/L NH4Cl, 0.1-0.5 mol/L CO (NH)2)2And (4) forming. The mineralized bacterium liquid is inoculated into the culture medium according to the inoculation amount of 1-5% for amplification culture, and then is uniformly sprayed on the sandy slope at one time.
The calcium source of the calcium source mixed solution is selected from calcium nitrate, calcium chloride, calcium acetate and the like, the concentration of calcium ions is between 0.25mol/L and 1mol/L, and the preferable range is between 0.25mol/L and 0.5 mol/L. The nutrient of the calcium source mixed solution comprises 0.2 mol/L-0.35 mol/L NH4Cl, 3% -5% NutrientBroth and 0.5-0.8 mol/L CO (NH)2)20.1 mol/L-0.2 mol/L NaHCO3. The calcium source mixed solution dissolves calcium source and nutrient water, the pH value of the calcium source mixed solution is 6-7, and natural rainfall, river water, well water, tap water, deionized water and the like can be adopted. The mixed solution of the grape bacterium liquid and the calcium source can be repeated for a plurality of times, so that the thickness of the solidified side slope is 5 cm-10 cm, and the solidified side slope is treated when the soil body is dried and cannot be subjected to post-treatment.
The research and development of the Bacillus liquid is the second key point of the invention, the Bacillus liquid adopts Bacillus sp, the registration number of the preservation center is CGMCC No.16169, the Bacillus liquid is preserved in the China general microbiological culture Collection center in 7-27 months in 2018, and the preservation and survival evidence is obtained. The Bacillus liquid is prepared by culturing Bacillus sp in a liquid culture medium, wherein the liquid culture medium is composed of 15-20 g of yeast powder, 10-15 g of cane sugar and 1000ml of distilled water. And spraying a layer of the bacillus liquid on the outer layer of the solidified layer on the side slope, and then covering a film for natural maintenance for 7 days.
As can be seen from the above, the microorganisms mineralizeThe cementing treatment fluid is a repairing agent with double effects of mineralization and cementing and is used for protecting the sandy slope. The microorganism used for preparing the treatment solution in the invention is not to destroy the original ecological environment of soil. The mineralized bacteria treating liquid is sprayed uniformly on the sandy soil slope which is easy to slide and has low cohesive force, so that CaCO3Depositing in the pores of the sandy soil particles, forming a layer of protective layer containing calcium carbonate deposition on the soil surface, spraying bacillus liquid with cementing capacity to be fused into the pores of the particles, and improving the cohesive force of the sandy slope soil particles, reducing the brittleness of the protective layer after the soil is solidified and enhancing the overall stability of the slope, wherein the solidification period is 5d and the natural maintenance period is 5 d.
Example 2
In this embodiment, the microbial mineralization cementing treatment fluid is composed of a mineralized bacteria treatment fluid with mineralization capability and a bacillus treatment fluid with cementing cohesiveness, and the preparation of the microbial mineralization cementing treatment fluid includes the following steps. The method comprises the following steps: preparing mineralized bacterium liquid.
The 2# strain of the mineralized bacteria is domesticated and cultured in a strain liquid culture medium for 3-4 times, the strain is inoculated into the culture medium according to the inoculation amount of 1% -5% every time, the culture time is 24-48 hours every time, the culture temperature is 30-40 ℃, the culture time is 180-200 r/min, the pH value is 6.0-6.5, and the culture time is 2 d-4 d.
The 2# bacterium liquid culture medium is prepared from 12-18 g/L yeast powder and 0.05-0.1 mol/L NH4Cl, 0.1-0.5 mol/L CO (NH)2)2And (4) forming.
Step two: preparing a calcium source mixed solution.
The calcium source mixed solution comprises a calcium source, nutrients and water.
Wherein the calcium source can be selected from calcium nitrate, calcium chloride, calcium acetate and the like, and the concentration of calcium ions is between 0.25mol/L and 1mol/L, and the preferred range is between 0.25mol and 0.5 mol.
The nutrient comprises 0.2 mol/L-0.35 mol/L NH4Cl, 3% -5% NutrientBroth and 0.5-0.8 mol/L CO (NH)2)20.1 mol/L-0.2 mol/L NaHCO3。
The water can be natural rainfall, river water, well water, tap water, deionized water and the like, and the pH value is 6-7.
Wherein the volume ratio of the mineralized bacterium liquid to the calcium source mixed solution is1 (10-50).
Step three: preparing bacillus liquid with cementing and cohering capacity.
The bacillus liquid adopts a desert self-source solidified strain with cementing and cohering capabilities, so that the cementing capability of sandy soil particles is improved; screening red clay 120-150 cm away from the ground surface from the inner Mongolia desert to obtain the desert self-source solidifying strain, namely Bacillus sp.
Wherein the liquid culture medium consists of 15-20 g of yeast powder, 10-15 g of sucrose and 1000ml of distilled water. The solid culture medium consists of 15-20 g of yeast powder, 10-15 g of ammonium sulfate, Tris15.748g, 15-20 g/L of agar powder and 1000ml of distilled water.
When the microorganism mineralized and cemented treatment fluid is used for treating and controlling sandy slopes which are easy to erode, the steps are as follows:
the method comprises the following steps: spraying mineralized bacterium liquid, inoculating the mineralized bacterium liquid into the culture medium in the first step according to the inoculation amount of 1% -5%, performing amplification culture, adding 20% -30% of fresh culture liquid, diluting and stirring uniformly to enable the OD600 value of the bacterium liquid to be 1.0-2.0, and spraying uniformly onto a sandy slope, wherein the spraying amount of the bacterium liquid is 300-400L/m3；
Step two: spraying the calcium source mixed solution in the step one, mixing and stirring the soluble calcium source solution, the nutrients and the aqueous solution in the step one uniformly, spraying the mixture to a sandy slope containing mineralized bacteria liquid in the step three, wherein the spraying amount of the calcium source mixed solution and the microbial bacteria liquid are sprayed according to the volume ratio of (10-50): 1, bacteria can generate urease in the metabolism process, when calcium ions with certain concentration are contained in the calcium source mixed solution, the calcium ions can be adsorbed by cells, and CaCO with the gelling effect is formed around the bacteria3Crystallizing, namely forming a calcium carbonate protective layer on the surface of the sand layer.
Step three: and (3) re-spraying, namely spraying the mineralized bacterium liquid and the calcium source mixed solution twice every day, wherein the spraying time interval of the mineralized bacterium liquid and the calcium source mixed solution is 0.5-1 h every time, continuously spraying for five days according to the third step and the fourth step, and spraying a bacillus treatment liquid on the outer layer of the solidified layer.
Furthermore, in order to protect the side slope from erosion and improve the treatment effect of mineralized bacteria, the grape bacteria liquid, the calcium source mixed solution and the bacillus liquid can be repeated for multiple times according to the actual soil quality and engineering requirements.
Example 3
Directly pouring mineralized bacteria (CGMCC No.15633) frozen in low temperature refrigerator, as shown in figure 3, into fresh culture medium (prepared from yeast powder 12-18 g/L and 0.05-0.1 mol NH)4Cl、0.1～0.5molCO(NH2) Composition) is cultured for 48 hours, the culture temperature is 30 ℃, 200r/min, the initial pH value is inoculated into a new culture medium according to the inoculum concentration of 1% each time, the activation culture is carried out for 3-4 times and 48 hours each time, then the culture bacterial liquid is expanded according to the area of the required protection slope, and the OD600 value of the activated bacterial liquid is adjusted to 1.0-1.5. The calcium source mixed solution mainly contains calcium chloride as calcium source, calcium ion concentration is 0.5mol, and 0.2-0.35 mol of NH is added4Cl、3％～5％NutrientBroth、0.5mol～0.8molCO(NH2)2、0.1mol～0.2mol NaHCO3And tap water.
The volume ratio of the mineralized bacterium liquid to the bacillus liquid with cementing capacity and the treatment liquid is1 (10-50); uniformly spraying the expanded culture solution on a sandy slope at one time, wherein the spraying amount of the bacteria solution is 400L/m as shown in figure 23And then uniformly mixing and stirring a soluble calcium source solution, a nutrient and an aqueous solution, and spraying the mixture onto a sandy slope containing mineralized bacteria liquid, wherein the spraying amount of the calcium source mixed solution and the microbial bacteria liquid are sprayed according to the volume ratio of (10-50) to 1, so that a calcium carbonate protective layer is formed on the surface of the sand layer.
In order to prevent the side slope from erosion and improve the side slope protection effect of the mineralized bacteria, the mineralized bacteria liquid and the calcium source mixed solution are sprayed twice each day, the calcium source mixed solution can be sprayed after the mineralized bacteria liquid is sprayed once, and the interval between the two times of spraying is 12 hours.
And after the steps are continuously sprayed for five days, spraying a layer of bacillus liquid with cementing capacity on the outer layer of the sandy soil after a solidified layer appears, and then naturally curing for five days. The spraying can be repeated according to the actual condition. The thickness of the solidified side slope reaches 5 cm-10 cm, and the side slope is treated when the soil body is dried in the protection process. The cured edge-broken protective layer has improved strength and no loose and sliding corrosion of particles, as shown in fig. 4.
The invention relates to a mineralized and cemented treatment fluid and an application method thereof in protection of sandy easily-eroded slopes3Crystallizing to achieve the purpose of solidifying the side slope. The mineralized bacteria are soil-derived strains, do not destroy the original ecological environment of soil, and are beneficial to mineralize loose sandy slopes to enable CaCO3The sediment is deposited in the sandy soil particle pores, a protective layer containing calcium carbonate is formed on the surface of the side slope, and spore bacteria liquid with cementing capacity is sprayed to be fused into the particle pores, so that the cohesive force of the sandy side slope soil particles is improved, the brittleness of the protective layer after the soil is solidified is reduced, and the overall stability of the side slope is improved.
The 2# bacterial liquid is suitable for sandy soil with the slope ratio of less than 1:1 and the particle size range of 0.074mm to d of less than 10mm, has good research and application prospects, and is suitable for large-scale popularization in protection of sandy soil slopes.
In conclusion, the microorganism mineralization cementation treatment fluid and the application method thereof in the protection of sandy easily-eroded side slope are characterized in that the used mineralization bacteria (Staphylococcus; gy1 #; preservation center registration number CGMCC No.15633) are screened from the soil, do not destroy the original ecological environment of the soil, and are used for mineralizing loose sandy side slope to enable CaCO3The solidified side slope is deposited in the pores of sandy soil particles, is matched with Bacillus liquid (Bacillus sp.; GiLi 1 #; registration number of preservation center is CGMCC No.16169) with cementing capacity to improve the cohesive force of the sandy side slope soil particles, is suitable for sandy soil with a side slope rate of less than 1:1 and a particle size range of 0.074mm < d < 10mm, has a thickness of 5 cm-10 cm, and is dried on a soil bodyThe invention has good research and application prospect, and is suitable for large-scale popularization of the solidification of the sandy slope soil.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Example 4
Referring to fig. 1, the method for preparing a microbial mineralized and cemented treatment fluid mainly includes the following steps:
firstly, preparing mineralized bacterial liquid with mineralization capacity;
secondly, preparing a calcium source nutrient solution;
and thirdly, preparing the bacillus mucilaginosus treating fluid with the cementing and cohering capacity.
The mineralized bacterium solution, the calcium source nutrient solution and the Bacillus mucilaginosus treatment solution are mutually independent, the OD600 value of the Bacillus mucilaginosus treatment solution is 0.6-0.8, the OD600 value of the Bacillus mucilaginosus treatment solution is 1.0-2.0, and the concentration of calcium ions in the calcium source nutrient solution is 0.25-1 mol/L; when in use, the volume ratio of the pasteurella bacillus treating liquid to the colloid bacillus treating liquid to the calcium source nutrient solution is1 (5-10) to 1-3.
The mineralized bacterium No.1 strain adopts the Bacillus pasteurianus, the mineralized bacterium strain is not the development of the invention, is available, and the invention only utilizes the mineralized bacterium strain. When the pasteurella bacillus treatment solution is prepared, the strain of pasteurella bacillus is adopted to be domesticated and cultured in a bacterial solution culture medium for 3-4 times, the strain is inoculated into the bacterial solution culture medium according to 1-5% of inoculation amount each time, the culture temperature is 30-40 ℃, the culture temperature is 180-200 r/min, the pH value is 6.0-6.5, and the culture time is 2 d-4 d. The bacterial liquid culture medium is prepared from yeast powder and (NH)4)2SO40.13M tris buffer solution and deionized water, wherein in each liter of deionized water, the yeast powder accounts for 20g/L, (NH)4)2SO410g/L of tris buffer, and 15.748g/L of 0.13M tris buffer. P of 0.13M Tris bufferH is 9.0.
In preparing the calcium source nutrient solution, a calcium source is dissolved in a nutrient to form the calcium source nutrient solution. The calcium source can be selected from calcium nitrate, calcium chloride, calcium acetate and the like, and the concentration of calcium ions is between 0.25 and 1mol, and the preferred range is between 0.25 and 0.5 mol. The nutrient may comprise 0.2mol to 0.35mol NH4Cl、3％～5％NutrientBroth、0.5mol～0.8molCO(NH2)2、0.1mol～0.2mol NaHCO3. The water required by the calcium source nutrient solution can be natural rainfall, river water, well water, tap water, deionized water and the like, and the pH value is 6-8.
When the Bacillus mucilaginosus treating fluid is prepared, a strain of Bacillus mucilaginosus is cultured in a liquid culture medium, the liquid culture medium is prepared by dissolving yeast powder and sucrose in distilled water, wherein the yeast powder accounts for 15-20 g/L and the sucrose accounts for 10-15 g/L in each liter of distilled water. The bacillus mucilaginosus is a soil self-source solidifying strain with cementing and cohering capabilities, and the cementing capability of sandy soil particles is improved; and screening red clay 120-150 cm away from the ground surface from the inner Mongolia desert to obtain the desert self-source solidifying strain.
Example 5
The embodiment discloses a method for protecting a sandy slope which is easy to erode, please refer to fig. 2, which includes the following steps:
1. the preparation method of the microbial mineralization cementation treatment fluid of the embodiment 4 is adopted;
2. and (3) protecting the sandy slope by using the microbial cementing treatment fluid.
In this embodiment, how to protect the sandy slope with the microbial cementing treatment fluid is described.
Spraying the bacillus pasteurii treating fluid of the microbial repairing agent on a sandy slope on the first day and the second day, wherein the spraying amount of the bacillus pasteurii treating fluid on the sandy slope every time is 200-400L/m3Spraying the calcium source nutrient solution of the microbial repairing agent, wherein the volume ratio of the pasteurella treatment solution to the calcium source nutrient solution is1 (1-3), repeatedly spraying the pasteurella treatment solution and the calcium source nutrient solution in the way,spraying twice every day for two days.
And on the third day, spraying the Bacillus mucilaginosus treating fluid of the microbial repairing agent, wherein the volume ratio of the Bacillus pasteurianus treating fluid to the Bacillus mucilaginosus treating fluid is1 (5-10).
And on the fourth day and the fifth day, spraying the pasteurella treatment solution and the calcium source nutrient solution twice every day for two days according to the first day and the second day.
On the sixth day, the Bacillus mucilaginosus treatment solution was sprayed, as referred to the third day.
Then natural curing is preferably carried out for five days.
In order to prevent the side slope from erosion and improve the treatment effect of the bacillus pasteurii, the calcium source nutrient solution and the bacillus solution can be repeatedly sprayed for a plurality of times according to the actual soil quality and engineering requirements. If the pasteurella treatment solution and the calcium source nutrient solution are repeatedly sprayed, the spraying is performed twice each day for two days, the spraying is performed for two days continuously, then the spraying of the bacillus mucilaginosus treatment solution is performed again, and the circulation is performed until the thickness of a solidified layer on the sandy slope is 5 cm-10 cm.
The bacillus pasteurii used in the invention belongs to the existing strain, namely Sporosarcina pasteurii, is purchased from American strain bank and is numbered as ATCC No.11859, does not destroy the original ecological environment of soil, is used for mineralizing loose sandy slopes and leading CaCO to be used3The sediment is deposited in the holes of the sand particles and is matched with colloidal bacillus liquid with cementing capacity. The colloidal Bacillus liquid is a new strain, Bacillus sp; GiLi 1 #; the preservation center registration number is CGMCC No.16169, improves cohesive force of sandy slope soil particles, is suitable for sandy soil with a slope rate of less than 1:1 and a particle size range d of less than 20mm, ensures that the solidified slope has a thickness of 5-10 cm, can be treated when the soil body is dry, and cannot be subjected to post-rain treatment.
Claims (7)
1. A microorganism mineralized cementation treatment fluid for treating sandy side slopes which are easy to erode is characterized by comprising mineralized bacterial liquid, colloidal bacillus bacterial liquid and calcium source mixed solution;
the mineralized bacterial liquid is divided into 1# pasteurella bacillus liquid and 2# staphylococcus liquid; the OD600 value of the 1# Pasteurella barnacii liquid is 0.6-0.8; the OD600 value of the 2# staphylococcus bacterium liquid is 1.0-2.0;
the bacillus liquid has cementing and cohering capacity, and the OD600 value is 1.0-2.0;
the calcium source mixed solution comprises a calcium source and nutrients, and the calcium ion concentration of the calcium source is between 0.25mol/L and 1 mol/L;
the mineralized bacterium liquid, the bacillus liquid and the calcium source mixed solution are mutually independent, and the mineralized bacterium liquid, the bacillus liquid and the calcium source mixed solution are sprayed in a volume ratio of 1 (5-10) to 1-3; when the microbial mineralization cementation treatment liquid is used, the spraying amount of the mineralized bacterial liquid is 300-400L/m3Spraying the mineralized bacterial liquid on a sandy slope, spraying the calcium source mixed solution on the sandy slope sprayed with the mineralized bacterial liquid, spraying the mineralized bacterial liquid and the calcium source mixed solution twice each day in this order, wherein the spraying time interval of the mineralized bacterial liquid and the calcium source mixed solution is 0.5-1 h every time, and continuously spraying the bacillus liquid for five days until a cured layer on the sandy slope reaches a certain thickness;
the mineralized bacterial liquid adopts mineralized strains which are domesticated and cultured in a culture medium for 3-4 times, the strains are inoculated into the culture medium according to the inoculation amount of 1% -5% every time, the culture temperature is 30-40 ℃, the culture temperature is 180-200 r/min, the pH value is 6.0-6.5, and the culture time is 2 d-4 d;
the culture medium of the 1# Pasteurella barnacles liquid comprises yeast powder, (NH4)2SO40.13M tris buffer solution and deionized water, wherein each liter of deionized water contains 20g/L yeast powder, (NH4)2SO410g/L of the buffer solution, and 15.748g/L of the 0.13M tris buffer solution;
the 2# staphylococcus liquid culture medium is prepared from 12-18 g/L yeast powder and 0.05-0.1 mol/L NH4Cl, 0.1-0.5 mol/L CO (NH)2)2And (4) forming.
2. The microbial mineralized cementitious treatment fluid according to claim 1, wherein the calcium source is selected from calcium nitrate, calcium chloride, and calcium acetate.
3. The microbial mineralized cementitious treatment fluid according to claim 1, wherein the nutrients comprise 0.2mol/L to 0.35mol/L NH4Cl, 3% -5% NutrientBroth and 0.5-0.8 mol/L CO (NH)2)20.1 mol/L-0.2 mol/L NaHCO3。
4. The microbial mineralization cementing treatment fluid for treating sandy side slope easy to erode as claimed in claim 1, wherein the calcium source mixed solution dissolves calcium source and nutrient water, and the pH value is 6-7.
5. A method for treating a sandy slope which is easy to erode is characterized by comprising the following steps:
providing a microbial mineralization cementing treatment fluid for treating a sandy slope according to any one of claims 1 to 4;
firstly, spraying a mineralized bacterial liquid of the microbial mineralization and cementation treatment liquid on a sandy slope;
spraying a calcium source mixed solution of the microorganism mineralization cementation treatment fluid on the sandy slope sprayed with the mineralization bacterial fluid;
spraying the mineralized bacterial liquid and the calcium source mixed solution twice every day in this sequence, wherein the spraying time interval of the mineralized bacterial liquid and the calcium source mixed solution is 0.5-1 h each time, continuously spraying for 5 days until a solidified layer on a sandy slope reaches a certain thickness, and then spraying the bacillus bacterial liquid of the microorganism mineralized and cemented treatment liquid;
wherein the spraying amount of the mineralized bacterial liquid is 300-400L/m3Spraying the mineralized bacterial liquid, the bacillus bacterial liquid and the calcium source mixed solutionThe volume ratio of the spraying is1 (5-10) to 1-3.
6. The method for protecting a sandy erodible sandy slope as claimed in claim 5 wherein the solidified layer has a thickness of from 5cm to 10 cm.
7. The method for protecting the sandy slope which is easy to erode and erode according to claim 6, wherein the method for protecting the 1# Pasteur bacillus liquid aims at sandy soil with slope rate less than 1:1 and particle size range d less than 20 mm;
or/and;
the method for treating the 2# grape ball bacterial liquid is suitable for sandy soil with the slope rate of less than 1:1 and the particle size range of 0.074mm < d < 10 mm.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007046557A1 (en) * | 2005-10-21 | 2007-04-26 | Jfe Steel Corporation | Slag material, method of producing the same, material for improving the environment using the slag material, environmental improvement method and structural engineering material |
| CN101525582A (en) * | 2008-03-07 | 2009-09-09 | 北京易众恒科技有限公司 | Multi-functional bacterial strain and application thereof |
| CN104403959A (en) * | 2014-09-18 | 2015-03-11 | 深圳大学 | Efficient calcium mineralized alkalophilic bacillus strain and application thereof |
| CN105625435A (en) * | 2014-10-30 | 2016-06-01 | 五冶集团上海有限公司 | Fast forming method for multi-section and multi-layer special-shaped equipment deep foundation pit of marine accumulation island |
| CN108130304A (en) * | 2018-02-10 | 2018-06-08 | 厦门昶青生物科技有限公司 | Bacillus composite bacterial liquid and preparation method thereof |
| CN108823259A (en) * | 2018-07-04 | 2018-11-16 | 内蒙古工业大学 | The calcareous glue of microorganism and preparation method, application, sand column and preparation method |
| CN108865889A (en) * | 2018-07-04 | 2018-11-23 | 内蒙古工业大学 | Method of the desert from the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability |
| CN108848732A (en) * | 2018-07-04 | 2018-11-23 | 内蒙古工业大学 | A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica |
| CN108929703A (en) * | 2018-07-04 | 2018-12-04 | 内蒙古工业大学 | A kind of preparation method of desert original position microorganism coating layer |
| KR20180138339A (en) * | 2017-06-21 | 2018-12-31 | 고려대학교 산학협력단 | Lysinibacillus sp. YS11 and use thereof |
-
2019
- 2019-01-15 CN CN201910037592.7A patent/CN109518703B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007046557A1 (en) * | 2005-10-21 | 2007-04-26 | Jfe Steel Corporation | Slag material, method of producing the same, material for improving the environment using the slag material, environmental improvement method and structural engineering material |
| CN101525582A (en) * | 2008-03-07 | 2009-09-09 | 北京易众恒科技有限公司 | Multi-functional bacterial strain and application thereof |
| CN104403959A (en) * | 2014-09-18 | 2015-03-11 | 深圳大学 | Efficient calcium mineralized alkalophilic bacillus strain and application thereof |
| CN105625435A (en) * | 2014-10-30 | 2016-06-01 | 五冶集团上海有限公司 | Fast forming method for multi-section and multi-layer special-shaped equipment deep foundation pit of marine accumulation island |
| KR20180138339A (en) * | 2017-06-21 | 2018-12-31 | 고려대학교 산학협력단 | Lysinibacillus sp. YS11 and use thereof |
| CN108130304A (en) * | 2018-02-10 | 2018-06-08 | 厦门昶青生物科技有限公司 | Bacillus composite bacterial liquid and preparation method thereof |
| CN108823259A (en) * | 2018-07-04 | 2018-11-16 | 内蒙古工业大学 | The calcareous glue of microorganism and preparation method, application, sand column and preparation method |
| CN108865889A (en) * | 2018-07-04 | 2018-11-23 | 内蒙古工业大学 | Method of the desert from the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability |
| CN108848732A (en) * | 2018-07-04 | 2018-11-23 | 内蒙古工业大学 | A kind of desert original position microorganism film coating technique and its green ecological administering method for combining salix monogolica |
| CN108929703A (en) * | 2018-07-04 | 2018-12-04 | 内蒙古工业大学 | A kind of preparation method of desert original position microorganism coating layer |
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