CN108865889A - Method of the desert from the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability - Google Patents
Method of the desert from the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability Download PDFInfo
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Abstract
The present invention discloses desert from the method for the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability.The separation method of bacterial strain is：From the aeolian sandy soil taken in the wild Ginkgo Biloba of Inner Mongol under 30~50cm of earth's surface, it is put into fluid nutrient medium and vibrates, it is prepared into the dilute solution of various concentration, it is respectively coated on solid medium, carry out constant temperature incubation, picking single colonie is crossed repeatedly, purifying culture 3-5 times, until single colonie occur and being selected as the desert from source mineralising bacterial strain.Fluid nutrient medium is by 10~15g yeast powder, 0.01~0.1molCH3COONa, 0.05~0.1mol NH4Cl, 0.1~0.5mol CO (NH2)2Composition.Solid medium is by 10~15g yeast powder, 0.01~0.1molCH3COONa, 0.05~0.1mol0.1NH4Cl, 0.1~0.5mol CO (NH2)2, 15~20g/L agar powder composition.
Description
Technical field
The present invention relates to the methods more particularly to a seed sand of a kind of separation method of bacterial strain and the consolidation sand of the bacterial strain
Desert is heavy in induction calcium carbonate from the separation method of source mineralising bacterial strain, the verification method of the mineralization ability of the bacterial strain, the bacterial strain
The method of application, bacterial strain inducing precipitation of calcium carbonate consolidation sand in consolidation of forming sediment sand.
Background technique
Soil is the optimum living environment of microorganism, is microorganism " natural medium ", microbial growth and
It is important that its metabolic activity causes mineral element migration, enrichment and microorganism to play in the diagenesis of deposit, mineralization
Effect.Organic matter soil abundant is that microorganism creates a living environment advantageously, and promoting microorganism secretion to go out can
With the mineralized material for being bonded together soil particle, and this microbial mineralization acts on the geotechnical engineering neck that is equally widely used
Domain combines bioengineering to repair and improve soil environment with geotechnical engineering.
Currently, country Southeast China University Qian Chunxiang《It is a kind of to consolidate loose sand particle using phosphate mineralized bacterium》
(ZL201310205389.9) it discloses and obtains phosphate ore using bacillus subtilis bacterium solution by being added disodium phenyl phosphate
Change bacterium, loose sand granular cementation is integral；Fang Xiangwei《Solidify the method for loose coral sand using microorganism》
(ZL20140050192.7) method for solidifying loose coral sand using Bacillus pasteurii is disclosed.In geotechnical engineering field,
Bacillus pasteurii is mainly used in method cured above, achieves preferable achievement, but at present in microorganism curing method
The strain used is more single, and microbe species are various in Aeolian Sandy Soil, can therefrom screen other bacterium with mineralization ability
Kind.
Summary of the invention
In view of this, the present invention proposes a kind of desert from the separation method of source mineralising bacterial strain, the mineralization ability of the bacterial strain
Application, the bacterial strain inducing precipitation of calcium carbonate in induction precipitation of calcium carbonate consolidation sand of verification method, the bacterial strain it is solid
The method for tying sand.
The present invention is implemented with the following technical solutions：A kind of desert is from the separation method of source mineralising bacterial strain, from Inner Mongol sand
The aeolian sandy soil under 30~50cm of earth's surface is taken in desert, is put into fluid nutrient medium and vibrates, and is prepared into the dilute solution of various concentration, point
It is not coated on solid medium, carries out constant temperature incubation, picking single colonie is crossed repeatedly, purifying culture 3-5 times, until occurring single
Bacterium colony is simultaneously selected as the desert from source mineralising bacterial strain；
Wherein, the fluid nutrient medium is by 10~15g yeast powder, 0.01~0.1molCH3COONa, 0.05~0.1mol
NH4Cl, 0.1~0.5mol CO (NH2)2Composition；The solid medium by 10~15g yeast powder, 0.01~
0.1molCH3COONa, 0.05~0.1mol0.1NH4Cl, 0.1~0.5mol CO (NH2)2, 15~20g/L agar powder composition.
As a further improvement of the foregoing solution, aeolian sandy soil, which is put into vibrate in fluid nutrient medium, is：
The aeolian sandy soil is put into fluid nutrient medium and stands for 24 hours after 15~60min of shaking；
Supernatant is taken to be seeded in the new fluid nutrient medium, 30 DEG C~35 DEG C of vibration temperature, 150~200r/min,
Duration 2d~3d, repeatedly shaken cultivation 3 times are cultivated in pH6.0~7.0；
Gradient dilution is at 10-1、10-2、10-3、10-4、10-5Dilution of the soil liquid of dilution as the various concentration
Solution.
As a further improvement of the foregoing solution, constant temperature incubation is：The dilute solution of various concentration is respectively coated on table
Face has 30 DEG C~35 DEG C constant temperature incubation 2d~3d on the plate of solid medium.
Further, the plate, which is put into constant incubator, cultivates.
As a further improvement of the foregoing solution, picking single colonie continues to be seeded in the new fluid nutrient medium, uses
Plate culture crosses separation repeatedly until there is the single colonie dispersed on plate repeatedly, and it is pure that progress is selected from the single colonie of dispersion
Change obtains the desert from source mineralising bacterial strain.
The present invention also provides a kind of verification method of the mineralization ability of bacterial strain, the bacterial strain is to use above-mentioned any desert certainly
The desert that the separation method of source mineralising bacterial strain is separated is from source mineralising bacterial strain；The verification method is：By different single colonies
It is put into after cultivating 2d~3d in fluid nutrient medium, is inoculated with 1~10ml bacterium solution into nutrient solution, the mixed liquor of white precipitate will occur
It stands for 24 hours, and white precipitate substance is identified by XRD；Wherein, the nutrient solution include 3%~6%Nutrient Broth,
0.4mol~0.7molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O, 0.1mol~0.5mol NH4Cl、0.01mol
~0.1mol NaHCO3。
The present invention also provides a kind of application of bacterial strain in induction precipitation of calcium carbonate consolidation sand, and the bacterial strain is using upper
The desert that the separation method for stating any desert from source mineralising bacterial strain is separated is from source mineralising bacterial strain.
The present invention also provides a kind of methods of bacterial strain inducing precipitation of calcium carbonate consolidation sand comprising step：
The sand for selecting partial size 0.074mm~2mm, sand and bacterium solution are mixed evenly and are prepared into sand sample, and
Immerse entire sand sample in nutrient solution, the growth for bacterial strain in bacterium solution provides required nutrition, makes bacterial strain in metabolism
Its mineralization ability is given full play in the process, is constantly induced precipitation of calcium carbonate in sand grains hole, is consolidated sand；Its
In, the bacterial strain is to be separated using the desert as described in any one of claim 1 to 5 from the separation method of source mineralising bacterial strain
Desert out is from source mineralising bacterial strain.
As a further improvement of the foregoing solution, the nutrient solution includes 3%~6%Nutrient Broth, 0.4mol
~0.7molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O, 0.1mol~0.5mol NH4Cl, 0.01mol~
0.1mol NaHCO3。
As a further improvement of the foregoing solution, as bacterial concentration OD600When being 0.6~2.5, sand probe intensity range
For 0.9~4.3MPa.
Bacterial strain disclosed by the invention with mineralization ability and its application in geotechnical engineering.This bacterial strain is staphylococcus
(Staphylococcus), collection registration number is CGMCC No.15633, and bacterial strain gy1# is oneself in Aeolian Sandy Soil
Source bacterial strain, the invention additionally provide the method for staphylococcus gy1# induction precipitation of calcium carbonate consolidation sand, CaCO3It is deposited in sand
In pellet pores, the cohesive strength of sand grains is improved, the original ecological environment of soil is not destroyed from source bacterial strain, can be used for ground work
The solidification of sand in journey has research and application prospect well, and is suitble to skinning and the improvement of large-scale promotion sandy soil earth.
Beneficial effects of the present invention：
1, gy1#, that is, desert can be used as a kind of microorganism fungus kind of new curing soil from source mineralising bacterial strain, and particle coheres
Effect is good, and solidification intensity is high, there is research and application value well.
2, gy1# is screened from source by Aeolian Sandy Soil and is separated, skinning and improvement suitable for sandy soil earth, can avoid external source
Influence of the strain to soil environment.
The preservation of biomaterial
Bacterial strain provided by the present invention is gy1#, is preserved in Chinese microorganism strain preservation pipe on April 18th, 2018
Reason committee common micro-organisms center (referred to as CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese science
Institute of microbiology of institute), collection registration number is CGMCC No.15633.
Detailed description of the invention
Fig. 1 is for desert from source mineralising bacterial strain application NJ method phylogenetic tree construction schematic diagram.
Fig. 2 is from the desert that the separation method of source mineralising bacterial strain separates from the SEM of source mineralising bacterial strain using desert of the present invention
Scanning electron microscope image.
Fig. 3 is using desert from the sand sample schematic diagram after the mineralising bacterial strain mineralising of source.
Fig. 4 is the X-ray diffraction analysis figure of sand sample in Fig. 3；
Fig. 5 is the SEM electron-microscope scanning image of sand sample in Fig. 3.
Fig. 6 is the energy spectrum analysis figure of sand sample in Fig. 3.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, not
For limiting the present invention.
In following embodiment, the solvent in all culture mediums, solution is pure water.
Embodiment 1
The present embodiment discloses a kind of separation method of desert from source mineralising bacterial strain.The bacterial strain is by Inner Mongol desert dust storm
Screening separation obtains in soil, through 16srDNA sequencing (being shown in Table 1) and phylogenetic tree construction, shows that this bacterial strain can be attributed to grape
Coccus (Staphylococcus) (phylogenetic tree is shown in Fig. 1), is named as gy1#.
Bacterial strain gy1# provided by the present invention is preserved in Chinese microorganism strain preservation management on April 18th, 2018
Committee's common micro-organisms center (referred to as CGMCC), collection registration number are CGMCC No.15633.The bacterium is gram
The positive, aerobic bacterium.
Table 1
That is, to what is picked out there is mineralization ability bacterial strain to carry out 16SrDNA sequencing and the building of systematic growth number.
The bacterial strain picked out can be obviously observed by scanning electron microscope：Thallus is uniform in size, average straight for ball-type or ellipse
Diameter is 1.2 μm~1.5 μm, sees Fig. 2.
16SrDNA identification is to carry out Species estimation, the present invention to bacterium using the method for the 16SrDNA sequence of bacterium
The sequencing of middle 16SrDNA is carried out by Sangon Biotech (Shanghai) Co., Ltd., and sequencing result is shown in Table 1.Using NJ
Method phylogenetic tree construction, is shown in Fig. 1, and the bacterial strain filtered out is attributed to staphylococcus (Staphylococcus), is named as
gy1#。
The separation method of the desert from source mineralising bacterial strain is：From the dust storm taken in the wild Ginkgo Biloba of Inner Mongol under 30~50cm of earth's surface
Soil is put into fluid nutrient medium and vibrates, and is prepared into the dilute solution of various concentration, is respectively coated on solid medium, carries out
Constant temperature incubation, picking single colonie are crossed repeatedly, purifying culture 3-5 times, until single colonie occur and being selected as the desert from source mine
Change bacterial strain.
Wherein, the fluid nutrient medium is by 10~15g yeast powder, 0.01~0.1molCH3COONa, 0.05~0.1mol
NH4Cl, 0.1~0.5mol CO (NH2)2Composition；The solid medium by 10~15g yeast powder, 0.01~
0.1molCH3COONa, 0.05~0.1mol0.1NH4Cl, 0.1~0.5mol CO (NH2)2, 15~20g/L agar powder composition.
In the present embodiment, desert from this Isolation and Culture of source mineralising bacterial strain and purifies：By separating and purifying technology from
The microorganism of needs is isolated in the natural microbial mixed.
The microorganism of needs is isolated from the natural microbial mixed by separating and purifying technology.From the wild Ginkgo Biloba of Inner Mongol
The sand of 30~50cm under selection of land table, take 1-5g be put into 100ml fluid nutrient medium (10~15g yeast powder, 0.01~
0.1CH3COONa, 0.05~0.1mol NH4Cl, 0.1~0.5mol CO (NH2)2) in stand after 15~60min of shake well
For 24 hours, supernatant is taken to be seeded in new fluid nutrient medium, 30 DEG C~35 DEG C of best vibration temperature, 150~200r/min, pH6.0
~7.0, cultivate duration 2d~3d.Repeatedly after shaken cultivation 3 times, gradient dilution is at 10-1、10-2、10-3、10-4、10-5It is dilute
The soil liquid for degree of releasing is respectively coated on plate (solid medium：10~15g yeast powder, 0.01~0.1mol CH3COONa、
0.05~0.1mol0.1NH4Cl, 0.1~0.5mol CO (NH2)2, 15~20g/L agar powder) upper 30 DEG C~35 DEG C constant temperature incubations
2d~3d.Picking single colonie continues to be seeded in new fluid nutrient medium, crosses separation repeatedly with plate culture repeatedly until flat
Occurs the single colonie dispersed on plate, and according to the shape of bacterium colony, lustrous surface, edge shape, the difference of the color of bacterium colony selects
Suitable bacterium colony is purified out.
Embodiment 2
It is consolidated from the bacterial strain that the separation method of source mineralising bacterial strain separates in induction precipitation of calcium carbonate using the desert of embodiment 1
Application in sand.Bacterial strain is the improvement and cured microorganism for sand, is a kind of bacterium with mineralization ability, and
The mechanical property that soil is improved in geotechnical engineering, has a good application prospect, and bacterial strain can be applied in induction precipitation of calcium carbonate consolidation
In sand.
Embodiment 3
The present embodiment discloses the mineralising energy of the bacterial strain separated using the desert of embodiment 1 from the separation method of source mineralising bacterial strain
The verification method of power, the verification method are：Different single colonies is put into after cultivating 2d~3d in fluid nutrient medium, inoculation 1
~10ml bacterium solution stands the mixed liquor for white precipitate occur for 24 hours into nutrient solution, and identifies white precipitate substance by XRD；Its
In, the nutrient solution includes 3%~6%Nutrient Broth, 0.4mol~0.7molCO (NH2)2, 0.25mol~
0.5molCaCl2.2H2O, 0.1mol~0.5mol NH4Cl, 0.01mol~0.1mol NaHCO3。
In the present embodiment, the verifying of the mineralization ability of bacterial strain chooses single colonie and detects its mineralization ability, and selecting has
The bacterial strain of mineralization ability.Different single colonies is put into respectively after cultivating 2d~3d in fluid nutrient medium, is inoculated with 1~10ml bacterium
Liquid stands the mixed liquor for white precipitate occur for 24 hours into nutrient solution, and identifies white precipitate substance by XRD etc..
Embodiment 4
The present embodiment discloses the bacterial strain separated using the desert of embodiment 1 from the separation method of source mineralising bacterial strain, the bacterium
The method of strain induction precipitation of calcium carbonate consolidation sand.
The method that bacterial strain inducing precipitation of calcium carbonate consolidates sand includes step：The sand of partial size 0.074mm~2mm is selected,
Sand and bacterium solution are mixed evenly and are prepared into sand sample, and immerses entire sand sample in nutrient solution, is in bacterium solution
The growth of bacterial strain provides required nutrition, and bacterial strain is made to give full play to its mineralization ability, continuous induced carbon in metabolic processes
Sour calcium precipitate consolidates sand in sand grains hole.
In the present embodiment, sand partial size 0.074mm~2mm is selected, sand and bacterium solution are mixed evenly and are prepared into
Sand sample, and entire test specimen is made to immerse nutrient solution (including 3%~6%Nutrient Broth, 0.4mol~0.7molCO
(NH2)2, 0.25mol~0.5molCaCl2.2H2O, 0.1mol~0.5mol NH4Cl, 0.01mol~0.1mol NaHCO3
Deng), required nutrition is provided for the growth of thallus, gy1# is made to give full play to its mineralization ability in metabolic processes, constantly
It induces precipitation of calcium carbonate in sand grains hole, consolidates sand, intensity is improved, and works as bacterial concentration OD600For
When 0.6~2.5, sand test piece intensity range is 0.9~4.3MPa.
Embodiment 5
Sand 150g~160g of partial size 0.074mm~2mm and 40ml bacterium solution are mixed uniformly, and are prepared into having a size of 50
The test specimen of × 50mm, and it is completely immersed in test specimen in solidification nutrient solution, it is passed through oxygen, microorganism is made to fully react with nutrient solution 5
~10 days, the sand test specimen after solidifying can be obtained, see Fig. 3.
In non-confining compression strength test, when OD600 is 2.5, intensity has reached 4.3MPa.Pass through the test specimen after solidifying
X-ray diffraction analysis is carried out, scanning angle is 20 °~80 °, and scanning mode is wide-angle angular instrument, is individually scanned, whiteness master
It to be CaCO3Crystal, crystal-type are A type calcite, see Fig. 4.By SEM electron-microscope scanning image, it is clear that sand grains
Surface and surrounding all there is CaCO3Crystallization, by CaCO between particle and particle3Bonding, the densification that entire sample becomes, and go out
Existing crystal overlapping phenomenon, is shown in Fig. 5.It can be seen that, there are C, O, Si, Cl, Ca element in the substance of generation by EDAX results,
And calcium constituent accounts for 40.25%, sees Fig. 6.
By means of the present invention, sand probe intensity and uniformity effects after a kind of novel grape coccus gy1# solidifies
Preferably, densification is connected between soil particle, cohesive strength increases, and higher integral strength.The present invention demonstrate extracted from soil it is novel
Staphylococcus gy1# is to have fine mineralization ability, and do not destroy the ecological ring of original soil from source bacterial strain in Aeolian Sandy Soil
Border improves the technical of the present invention, is suitble to skinning and the improvement of large-scale promotion sandy soil earth.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of desert is from the separation method of source mineralising bacterial strain, which is characterized in that take 30~50cm of earth's surface from the wild Ginkgo Biloba of Inner Mongol
Under aeolian sandy soil, be put into fluid nutrient medium and vibrate, be prepared into the dilute solution of various concentration, be respectively coated on solid medium
On, constant temperature incubation is carried out, picking single colonie is crossed repeatedly, purifying culture 3-5 times, until single colonie occur and being selected as the desert
From source mineralising bacterial strain；
Wherein, the fluid nutrient medium is by 10~15g yeast powder, 0.01~0.1molCH3COONa, 0.05~0.1mol
NH4Cl, 0.1~0.5mol CO (NH2)2Composition；The solid medium by 10~15g yeast powder, 0.01~
0.1molCH3COONa, 0.05~0.1mol0.1NH4Cl, 0.1~0.5mol CO (NH2)2, 15~20g/L agar powder composition.
2. desert as described in claim 1 is from the separation method of source mineralising bacterial strain, which is characterized in that aeolian sandy soil is put into liquid training
Supporting oscillation in base is：
The aeolian sandy soil is put into fluid nutrient medium and stands for 24 hours after 15~60min of shaking；
Supernatant is taken to be seeded in the new fluid nutrient medium, 30 DEG C~35 DEG C of vibration temperature, 150~200r/min, pH6.0
~7.0, cultivate duration 2d~3d, repeatedly shaken cultivation 3 times；
Gradient dilution is at 10-1、10-2、10-3、10-4、10-5Dilute solution of the soil liquid of dilution as the various concentration.
3. desert as described in claim 1 is from the separation method of source mineralising bacterial strain, which is characterized in that constant temperature incubation is：It is different
The dilute solution of concentration is respectively coated on upper surface with 30 DEG C~35 DEG C constant temperature incubation 2d~3d on the plate of solid medium.
4. desert as claimed in claim 3 is from the separation method of source mineralising bacterial strain, which is characterized in that the plate is put into constant temperature
It is cultivated in incubator.
5. desert as described in claim 1 is from the separation method of source mineralising bacterial strain, which is characterized in that picking single colonie is after continued access
Kind crosses separation repeatedly until there is the single bacterium dispersed on plate repeatedly into the new fluid nutrient medium, with plate culture
It falls, is selected from the single colonie of dispersion and purified to obtain the desert from source mineralising bacterial strain.
6. a kind of verification method of the mineralization ability of bacterial strain, which is characterized in that the bacterial strain is using as in claim 1 to 5
The desert that the separation method of desert described in any one from source mineralising bacterial strain is separated is from source mineralising bacterial strain；The authentication
Method is：Different single colonies is put into after cultivating 2d~3d in fluid nutrient medium, is inoculated with 1~10ml bacterium solution into nutrient solution, it will
The mixed liquor for white precipitate occur is stood for 24 hours, and identifies white precipitate substance by XRD；Wherein, the nutrient solution include 3%~
6%Nutrient Broth, 0.4mol~0.7molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O, 0.1mol~
0.5mol NH4Cl, 0.01mol~0.1mol NaHCO3。
7. a kind of application of bacterial strain in induction precipitation of calcium carbonate consolidation sand, which is characterized in that the bacterial strain is using as weighed
Benefit require any one of 1 to 5 described in desert from source mineralising bacterial strain the desert separated of separation method from source mineralized bacterium
Strain.
8. a kind of method of bacterial strain inducing precipitation of calcium carbonate consolidation sand, which is characterized in that it includes step：
The sand for selecting partial size 0.074mm~2mm, sand and bacterium solution are mixed evenly and are prepared into sand sample, and is made whole
A sand sample immerses in nutrient solution, and the growth for bacterial strain in bacterium solution provides required nutrition, makes bacterial strain in metabolic processes
In give full play to its mineralization ability, constantly induce precipitation of calcium carbonate in sand grains hole, consolidate sand；Wherein,
The bacterial strain is to be isolated using the separation method of desert from source mineralising bacterial strain as described in any one of claim 1 to 5
The desert come is from source mineralising bacterial strain.
9. the method for bacterial strain inducing precipitation of calcium carbonate consolidation sand as claimed in claim 8, which is characterized in that the nutrient solution
Including 3%~6%Nutrient Broth, 0.4mol~0.7molCO (NH2)2, 0.25mol~0.5molCaCl2.2H2O、
0.1mol~0.5mol NH4Cl, 0.01mol~0.1mol NaHCO3。
10. the method for bacterial strain inducing precipitation of calcium carbonate consolidation sand as claimed in claim 8, which is characterized in that when bacterium solution is dense
Spend OD600When being 0.6~2.5, sand probe intensity range is 0.9~4.3MPa.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109518703A (en) * | 2019-01-15 | 2019-03-26 | 内蒙古工业大学 | A kind of administering method of the cementing treatment fluid of microbial mineralization and chiltern side slope |
| CN109576336A (en) * | 2019-01-15 | 2019-04-05 | 内蒙古工业大学 | A kind of Bacillus pasteurii combines solid indigenous method with cementing bacillus |
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| CN109629360A (en) * | 2018-11-26 | 2019-04-16 | 温州大学 | The method that clay roadbed is reinforced using carbon sequestration bacterium mineralising |
| CN109629360B (en) * | 2018-11-26 | 2021-02-26 | 温州大学 | Method for mineralizing and reinforcing clay roadbed by carbon-fixing bacteria |
| CN109518703A (en) * | 2019-01-15 | 2019-03-26 | 内蒙古工业大学 | A kind of administering method of the cementing treatment fluid of microbial mineralization and chiltern side slope |
| CN109576336A (en) * | 2019-01-15 | 2019-04-05 | 内蒙古工业大学 | A kind of Bacillus pasteurii combines solid indigenous method with cementing bacillus |
| CN109576193A (en) * | 2019-01-15 | 2019-04-05 | 内蒙古工业大学 | A kind of microorganism remediation liquid and its application method in chiltern foundation pit slope protection |
| CN109666610A (en) * | 2019-01-15 | 2019-04-23 | 内蒙古工业大学 | Desert solidifies the separation method and its solidification sand proficiency testing method of bacterial strain from source |
| CN109576193B (en) * | 2019-01-15 | 2021-06-18 | 内蒙古工业大学 | Microbial remediation liquid and application method thereof in protection of sandy foundation pit slope |
| CN109518703B (en) * | 2019-01-15 | 2021-07-02 | 内蒙古工业大学 | Microbial mineralization and cementation treatment fluid and treatment method of sandy slope |
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