CN100357444C - Preparation of calcium carbonate by microbe deposition - Google Patents
Preparation of calcium carbonate by microbe deposition Download PDFInfo
- Publication number
- CN100357444C CN100357444C CNB2005100947445A CN200510094744A CN100357444C CN 100357444 C CN100357444 C CN 100357444C CN B2005100947445 A CNB2005100947445 A CN B2005100947445A CN 200510094744 A CN200510094744 A CN 200510094744A CN 100357444 C CN100357444 C CN 100357444C
- Authority
- CN
- China
- Prior art keywords
- lime carbonate
- deposition
- calcium carbonate
- solution
- bacterium liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention discloses a method for preparing calcium carbonate by microorganism deposition. Bacterial strains of Bacillus pasteurii are inoculated to a culture medium containing urea substrate to be in shake cultivation at the temperature of 25 to 37 DEG C, initial pH value is regulated and controlled between 6.0 to 8.0 for the cultivation for 12 to 24 hours, bacterium liquid is taken out, CaCl2 solution is incorporated, generated precipitates are taken out, and the precipitate are filtered and dried to obtain calcium carbonate. The present invention has the advantages of abound resources, simple technology, clean environment, low cost, high purity of prepared calcium carbonate by deposition, high production rate, etc. The present invention can fully utilize microorganism resources in the natural world, be used as fillings, be used for repairing building cracks, and be used as cementing of reclaimed concrete.
Description
Technical field
The present invention relates to a kind of preparation method of lime carbonate, especially a kind of method of utilizing microbe deposition to prepare lime carbonate.
Background technology
Lime carbonate is a kind of important inorganic chemical product, is widely used in industries such as rubber, plastics, printing ink, papermaking, coating, sealing agent, tackiness agent, daily necessities, makeup, food and feed and medicine.At present its preparation technology mainly is divided into mechanical crushing method and chemical reaction method, and positive sternness is faced with that resource is weary frequently, problems such as energy consumption is big, dust is many, product specification is not enough, quality instability in the former production process; And the latter also exists complex process in process of production, and energy consumption is big, the fume emission problem.Therefore, problems such as the resource scarcity that exists in traditional lime carbonate industrial production process, environmental pollution, ecological damage can not be ignored.
Biomineralization is a kind of very general spontaneous phenomenon, and almost each biology can both synthetic mineral.Biomineralization often can form ordered arrangement, that structure is very excellent is natural organic---inorganic composite materials, wherein nearly 2nd/3rd, calcium ore deposit, and quite a few has glued function.They are by the vital movement of himself, and between the surrounding environment medium constantly circulation enzyme is taking place is turning usefulness into, mineralising forms a kind of cementing material gradually---calcite (lime carbonate), pass through the accumulation in very long period again, the most at last the hard rock of the glued formation of the sedimentary loose detrital material of occurring in nature.
Summary of the invention
The invention provides a kind of biological enzyme that utilizes and turn usefulness into, the artificial microbe deposition that utilizes that quickens deposition preparation lime carbonate prepares the method for lime carbonate.
The present invention adopts following technical scheme:
A kind of method of utilizing microbe deposition to prepare lime carbonate, bacterial strain Pasteur bacillus Bacilluspasteurii is seeded to is equipped with on the substratum that contains the urea substrate, under 25～37 ℃, carry out shaking culture, and initial pH is controlled at 6.0～8.0, cultivated 12～24 hours, take out bacterium liquid, mix CaCl
2Solution, last, will produce precipitation and take out, filter, dry, can obtain lime carbonate.
The present invention is seeded to bacterial strain Bacillus pasteurii in the substratum, is nutrition source with the substrate urea in the substratum, produces urase, continuous decomposing urea, cause the suitable rising of pH value in the solution, be more conducive to the growth and breeding of thalline, enzyme turns into also being able to continuous enhancing.In this working cycle, substrate urea constantly decomposes, and makes the CO in the solution
3 2-Concentration constantly increases.In solution, introduce Ca this moment
2+, the continuous immediately chelating Ca of SM (water soluble organic matter) that somatic cells membrane interface place is electronegative
2+, induce partial crystal negatively charged ion (CO
3 2-) concentration further increases, thereby attract more Ca
2+, increase to up to crystal precursor concentration and to be beneficial to coring, deposit CaCO
3Particle, entire reaction is suc as formula shown in 1～formula 3:
The bacterial classification enzymeization
(NH
2)
2CO+2H
2O————→CO
3 2-+2NH
4 + (1)
Cell+Ca
2+＝Cell-Ca
2+ (2)
Cell-Ca
2++CO
3 2-＝Cell-CaCO
3 (3)
Compared with prior art, the present invention has following advantage:
The present invention makes full use of the nature microorganism resource, by the mineralization of biology, quickens deposition preparation lime carbonate, and not only aboundresources, and technology is simple, and clean environment is with low cost, and the lime carbonate purity height of the preparation that deposits, productive rate are also up to arriving nearly 90%.Room temperature condition can deposit the calcite crystal form calcium carbonate that obtains regular spherical down, not only can be used as filler and uses, and can also be used for the repairing of building tear, the gluing of regeneration concrete etc.; 50 ℃～70 ℃ can deposit the meta crystal formation vaterite that obtains lime carbonate down; Introduce nucleator MgSO
4Behind the solution, and adopt the magnetic alr mode, sedimentary calcium carbonate granule size minimum is refined to about 0.3 μ m.
Description of drawings
Fig. 1 is microbe deposition CaCO under the room temperature
3The X-ray diffraction analysis collection of illustrative plates.
Fig. 2 is microbe deposition CaCO under the room temperature
3Observed granule-morphology under scanning electronic microscope, wherein, (a) CaCO
3Particle overall distribution situation; (b) CaCO of surface attachment thalline
3Particle.
Fig. 3 is microbe deposition CaCO under 50 ℃～70 ℃ conditions of constant temperature
3The X-ray diffraction analysis collection of illustrative plates, wherein, A is a vaterite, B is a calcite.
Fig. 4 is microbe deposition CaCO under 50 ℃～70 ℃ conditions of constant temperature
3Observed granule-morphology under scanning electronic microscope wherein, is spherical, square CaCO3 granule-morphology (calcite) (a), (b) is fusiform CaCO3 granule-morphology (vaterite).
Fig. 5 is nucleator MgSO
4Introduce back microbe deposition CaCO
3The X-ray diffraction analysis collection of illustrative plates.
Fig. 6 is nucleator MgSO
4Introduce back microbe deposition CaCO
3Observed granule-morphology under scanning electronic microscope.
Embodiment
A kind of method of utilizing microbe deposition to prepare lime carbonate, bacterial strain Pasteur bacillus Bacilluspasteurii is seeded to is equipped with on the substratum that contains the urea substrate, under 25～37 ℃, carry out shaking culture, and initial pH is controlled at 6.0～8.0, cultivated 12～24 hours, take out bacterium liquid, mix CaCl
2Solution, last, will produce precipitation and take out, filter, dry, can obtain lime carbonate, in the present embodiment, the temperature of shaking culture can be selected 25,30 or 37 ℃, and initial pH is controlled at 6.0,7.0 or 8.0, and incubation time can be selected 12,15,17,20 or 24 hours.In addition, present embodiment can be heated to 50 ℃～70 ℃ (as: 50 ℃, 70 ℃, 55 ℃ or 60 ℃) with the bacterium liquid that takes out, and mixes CaCl under 50 ℃～70 ℃
2Solution; Present embodiment also can mix CaCl under room temperature after taking out bacterium liquid
2Solution; Present embodiment can also mix the MgSO as nucleator in the bacterium liquid that takes out
4Solution, and adopt magnetic to stir 5～10min, concrete magnetic churning time can select 5,7,9 or 10min for use.The composition of above-mentioned substratum and proportioning are: peptone: meat extract: agar: urea: distilled water=0.005: 0.003: 0.015: 0.01～0.10: 1.
Bacterial strain Bacillus pasteurii is seeded to (medium component is as shown in table 1) in the triangular flask that substratum is housed, 25～37 ℃ of shaking culture in the constant-temperature shaking culture case, initial pH condition is controlled at 6.0～8.0, cultivates taking-up in 12～24 hours, mixes CaCl under the normal temperature
2Solution produces precipitation rapidly, with its filtration, oven dry, is calcite type calcium carbonate (Fig. 1) through X-ray diffraction analysis, and purity is very high, and granule-morphology as shown in Figure 2, and is spherical in shape, and particle surface is also adhering to remaining thalline.
Table 1 medium component
| Nutritive substance | Quality (g) | Main effect |
| Peptone meat extract agar urea distilled water | 5.0 3.0 15.0 20.0 1000.0 | Nitrogenous source is provided, carbon source (energy), somatomedin provides nitrogenous source, carbon source (energy), inorganic salt, mix strains A enzyme reaction participation substrate during somatomedin preparation solid medium moisture is provided |
Bacterial strain Bacillus pasteurii is seeded to (medium component is as shown in table 1) in the triangular flask that substratum is housed, 25～37 ℃ of shaking culture in the constant-temperature shaking culture case, initial pH condition is controlled at 6.0～8.0, cultivate taking-up in 12～24 hours, place 50 ℃～70 ℃ Water Tanks with Temp.-controlled, mix CaCl
2Solution produces rapidly precipitation, with its filtration, oven dry, is lime carbonate through X-ray diffraction analysis, and purity is very high, presents two kinds of crystal formations of vaterite and calcite (Fig. 3), granule-morphology as shown in Figure 4, multiple patterns such as spherical in shape, square, fusiform.
Embodiment 4
Bacterial strain Bacillus pasteurii is seeded to (medium component is as shown in table 1) in the triangular flask that substratum is housed, 25～37 ℃ of shaking culture in the constant-temperature shaking culture case, initial pH condition is controlled at 7.0, cultivates taking-up in 12～24 hours, mixes CaCl under the normal temperature
2Solution, and with MgSO
4Solution also mixes wherein as nucleator, adopt magnetic to stir 5～10min, produce precipitation rapidly, its filtration, oven dry, through X-ray diffraction analysis is calcite type calcium carbonate (Fig. 5), purity is very high, and granule-morphology as shown in Figure 6, and is spherical in shape, particle surface is fine and smooth, better dispersed, there is not agglomeration, grain diameter is decreased to about 0.3～1 μ m.
Claims (4)
1, a kind of method of utilizing microbe deposition to prepare lime carbonate, it is characterized in that bacterial strain Pasteur bacillus Bacillus pasteurii is seeded to and be equipped with on the substratum that contains the urea substrate, under 25～37 ℃, carry out shaking culture, and initial pH is controlled at 6.0～8.0, cultivated 12～24 hours, take out bacterium liquid, mix CaCl
2Solution, last, will produce precipitation and take out, filter, dry, can obtain lime carbonate.
2, the method for utilizing microbe deposition to prepare lime carbonate according to claim 1 is characterized in that taking-up bacterium liquid is heated to 50 ℃～70 ℃, and mix CaCl under 50 ℃～70 ℃
2Solution.
3, the method for utilizing microbe deposition to prepare lime carbonate according to claim 1, it is characterized in that taking out bacterium liquid after, under room temperature, mix CaCl
2Solution.
4, the method for utilizing microbe deposition to prepare lime carbonate according to claim 3 is characterized in that mixing the MgSO as nucleator in the bacterium liquid that takes out
4Solution, and adopt magnetic to stir 5～10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100947445A CN100357444C (en) | 2005-10-10 | 2005-10-10 | Preparation of calcium carbonate by microbe deposition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100947445A CN100357444C (en) | 2005-10-10 | 2005-10-10 | Preparation of calcium carbonate by microbe deposition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1778934A CN1778934A (en) | 2006-05-31 |
| CN100357444C true CN100357444C (en) | 2007-12-26 |
Family
ID=36769389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100947445A Active CN100357444C (en) | 2005-10-10 | 2005-10-10 | Preparation of calcium carbonate by microbe deposition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100357444C (en) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302484B (en) * | 2008-01-15 | 2010-11-24 | 东南大学 | Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid |
| CN101234909B (en) * | 2008-02-27 | 2010-06-16 | 东南大学 | Cement-base material microorganism tectorial membrane protection agent, preparing method and application thereof |
| US11795108B2 (en) | 2016-10-31 | 2023-10-24 | Biomason Inc. | Microorganism loaded aggregate and manufacturing methods |
| US10717674B2 (en) | 2010-04-27 | 2020-07-21 | Biomason, Inc. | Methods for the manufacture of colorfast masonry |
| CN102139278A (en) * | 2010-12-14 | 2011-08-03 | 东南大学 | Microbial preparation for mineralized and consolidated copper ions and use method thereof |
| CN102493494B (en) * | 2011-12-12 | 2013-09-04 | 山东建筑大学 | Method for sealing vertical cracks of basement concrete structures by using microbial grouting |
| CN102493493B (en) * | 2011-12-12 | 2013-08-28 | 山东建筑大学 | Method for plugging horizontal fissures of basement concrete structure by grouting microorganisms |
| CN102531432A (en) * | 2012-01-16 | 2012-07-04 | 东南大学 | Microbial gelled material and method for forming magnesite by using same to glue sand grains |
| CN103342484B (en) * | 2013-07-18 | 2014-09-03 | 东南大学 | Method for repairing crack of cement-based material |
| CN104196131B (en) * | 2014-09-11 | 2016-06-01 | 山东建筑大学 | The cast-in-place slab-type apartment building plate of microbiological precipitation of CaCO 3 blocking concrete or soleplate crack method |
| CN104609453B (en) * | 2014-11-25 | 2016-08-24 | 广西华纳新材料科技有限公司 | A kind of preparation method of cubic winnofil |
| CN105837075B (en) * | 2015-07-07 | 2018-04-20 | 东南大学 | A kind of method using microbiological precipitation of CaCO 3 intensifying regenerating concrete fine aggregate |
| CN106193128B (en) * | 2016-07-11 | 2018-09-07 | 东南大学 | Obstruct the repair apparatus and its method of the clay coating cracking of organic pollution volatilization |
| CN106241848B (en) * | 2016-08-30 | 2017-08-15 | 广东高航知识产权运营有限公司 | A kind of method that utilization paramecium prepares high dispersion of nano-calcium carbonate |
| CN106701100B (en) * | 2017-01-09 | 2019-03-29 | 中国神华能源股份有限公司 | A kind of method of Salty Soil Subgrade filler and improvement roadbed |
| CN107083847B (en) * | 2017-04-21 | 2019-08-23 | 广州新城建筑设计院有限公司 | A method of masonry is repaired using oyster shell and microorganism |
| MX2020003991A (en) | 2017-10-18 | 2020-10-07 | Cemex Innovation Holding Ltd | Method to design a self-healing concrete additionated with a permeable concrete containing biological materials. |
| CN108441442A (en) * | 2018-03-14 | 2018-08-24 | 东南大学 | A method of it directly extracting microorganism fungus kind from soil and prepares calcium carbonate |
| CN108404521A (en) * | 2018-04-08 | 2018-08-17 | 赵建平 | A kind of preparation method of acupuncture filter material |
| CN108315355B (en) * | 2018-04-25 | 2023-12-01 | 河海大学 | Method and device for synthesizing stalactite and stalagmite by microorganisms |
| CN108558260B (en) * | 2018-05-24 | 2020-12-01 | 中交四航工程研究院有限公司 | Self-healing concrete complex enzyme microcapsule material and preparation method thereof |
| CN108841870A (en) * | 2018-06-04 | 2018-11-20 | 天津大学 | The preparation method of calcium carbonate |
| CN109295108B (en) * | 2018-09-14 | 2022-02-15 | 天津科技大学 | Method for preparing calcium carbonate with different crystal forms by utilizing microbial mineralization |
| CN109517753B (en) * | 2018-11-14 | 2022-03-08 | 南京师范大学 | Biological vaterite and preparation method and application thereof |
| CN109610658A (en) * | 2018-12-19 | 2019-04-12 | 江苏镇江建筑科学研究院集团股份有限公司 | A method of utilizing microorganism induction tosca repairing concrete crack |
| CN113710290B (en) * | 2019-02-15 | 2024-03-19 | 拜奥梅森股份有限公司 | Non-caloric or non-pressure biosintering |
| CN110860216B (en) * | 2019-10-25 | 2021-10-01 | 江苏大学 | Preparation process and preparation method of microporous inorganic filter membrane |
| CN110951816A (en) * | 2019-11-29 | 2020-04-03 | 东南大学 | Method for regulating grain size of calcite induced and deposited by microorganisms |
| CN111792661B (en) * | 2020-07-29 | 2021-12-14 | 华南农业大学 | Submicron spherical biological calcium carbonate and preparation method and application thereof |
| CN112626160A (en) * | 2020-12-23 | 2021-04-09 | 东南大学 | Method for regulating and controlling adhesion force of calcite deposited by microorganism induction |
| CN113884351B (en) * | 2021-09-03 | 2023-10-27 | 中国矿业大学 | Preparation method of magnetic sensitivity simulated lunar soil based on biological curing technology |
| CN113943125B (en) * | 2021-09-06 | 2023-06-06 | 北京航天恒丰科技股份有限公司 | Microbial agent crack repairing liquid for repairing blast furnace cracks |
| CN115448346B (en) * | 2022-08-11 | 2024-03-12 | 中南大学 | Preparation method of superfine light calcium carbonate |
| CN116140165B (en) * | 2023-02-28 | 2023-08-29 | 上海海事大学 | Biomineralization coating capable of being rapidly synthesized and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1509144A (en) * | 2001-05-15 | 2004-06-30 | \ | Prebiotic and probiotic compositions and method for use in gut-based therapies |
-
2005
- 2005-10-10 CN CNB2005100947445A patent/CN100357444C/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1509144A (en) * | 2001-05-15 | 2004-06-30 | \ | Prebiotic and probiotic compositions and method for use in gut-based therapies |
Non-Patent Citations (2)
| Title |
|---|
| 尿素水解法制备晶须碳酸钙 许兢等.结构化学，第22卷第2期 2003 * |
| 微生物沉积碳酸钙研究 王瑞兴，钱春香，王剑云.东南大学学报（自然科学版），第35卷第增刊1期 2005 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1778934A (en) | 2006-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100357444C (en) | Preparation of calcium carbonate by microbe deposition | |
| Ng et al. | An overview of the factors affecting microbial-induced calcite precipitation and its potential application in soil improvement | |
| Ivanov et al. | Basics of construction microbial biotechnology | |
| Chen et al. | Microbial induced calcium carbonate precipitation (MICP) using pig urine as an alternative to industrial urea | |
| CA2803512C (en) | Biocementation of particulate material in suspension | |
| CN109295108A (en) | The method for preparing different crystal system calcium carbonate using microbial mineralization | |
| CN1923720A (en) | Method of consolidating heavy metal ion by microorganism | |
| CN108220380A (en) | The method for preparing calcium carbonate using two kinds of common mineralisings of microorganism | |
| CN115304297A (en) | Microorganism-loaded aggregates and methods of manufacture | |
| CN110964533B (en) | Composition for preventing soil loss by inducing precipitation of calcium carbonate using microorganism and method for preventing soil loss using same | |
| CN105779356B (en) | A kind of microorganism self-assembled nanometer material and its preparation method and application | |
| Anitha et al. | Bacillus cereus KLUVAA mediated biocement production using hard water and urea | |
| CN108865889A (en) | Method of the desert from the separation method of source mineralising bacterial strain, the verification method and consolidation sand of mineralization ability | |
| CN109942347A (en) | A method of making microbial manure using coal gangue as raw material | |
| CN112626160A (en) | Method for regulating and controlling adhesion force of calcite deposited by microorganism induction | |
| CN104136625A (en) | Method for rapidly and efficiently harvesting green algae using cationic organoclay | |
| CN109110928A (en) | The cultural method of biomembrane on a kind of biologic packing material | |
| Ibrahim et al. | A Literature Review of Bio-cement: Microorganisms, Production, Properties, and Potential Applications. | |
| KR102028285B1 (en) | Method for fixing carbon dioxide using extracellular polymeric substance from microbial growth, and method for reparing microcrack using the extracellular polymeric substance | |
| CN105833832B (en) | A kind of biochemical preparation for administering glutamic acid fermentation sewage | |
| CN104004690A (en) | Nitrobacteria and culturing method for nitrobacteria | |
| CN102139278A (en) | Microbial preparation for mineralized and consolidated copper ions and use method thereof | |
| CN110951816A (en) | Method for regulating grain size of calcite induced and deposited by microorganisms | |
| CN106045201B (en) | The biological treatment of xanthan gum fermentation waste | |
| CN102051382B (en) | Method for preparing spherical barium carbonate powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |