CN112376584A - Sand slope grouting reinforcement method based on combined action of vegetation rhizome and microorganism - Google Patents
Sand slope grouting reinforcement method based on combined action of vegetation rhizome and microorganism Download PDFInfo
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- CN112376584A CN112376584A CN202011109077.4A CN202011109077A CN112376584A CN 112376584 A CN112376584 A CN 112376584A CN 202011109077 A CN202011109077 A CN 202011109077A CN 112376584 A CN112376584 A CN 112376584A
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D17/00—Excavations; Bordering of excavations; Making embankments
- E02D17/20—Securing of slopes or inclines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a sandy slope grouting reinforcement method based on the joint action of vegetation rhizomes and microorganisms, which comprises the following steps: planting a gramineous plant on the slope surface of the sandy slope, cutting off branches at the upper part of the gramineous plant after the gramineous plant naturally withers, reserving a gramineous plant stem with a certain height, and marking a first gramineous plant branch stem and a second gramineous plant branch stem; pouring a mixture of a microbial liquid and a microbial culture solution through a branch stem conduit of the first gramineous plant; and (3) pouring a microbial nutrient solution through the branch stem conduit of the second gramineous plant, wherein the microbial nutrient solution is converged with the microbial solution and the microbial culture solution at the main stem conduit of the gramineous plant and reaches each root branch along with the conduit of the root system. The method has the advantages of low construction cost, environmental friendliness, good curing effect and good mechanical property, and can obviously improve the overall stability of the sandy side slope, thereby achieving the purpose of preventing and controlling the disaster of the side slope.
Description
Technical Field
The invention belongs to the technical field of sandy slope reinforcement, relates to a sandy slope reinforcement method, and particularly relates to a sandy slope grouting reinforcement method based on the joint action of vegetation rhizomes and microorganisms.
Background
The sand side slope has large pores among sand grains, loose structure, poor adhesive force, low shear strength and poor water retention, is widely distributed on two sides of highways, railways, riverways and the like, and is easy to have engineering accidents such as landslide, collapse and the like under the action of external force (rainstorm, earthquake) and even cause economic loss and casualties. Therefore, how to effectively reinforce the sandy slope has become an important topic in the field of geotechnical engineering.
At present, the commonly used sand slope reinforcement methods include surface grouting, anchor rod reinforcement, soil nail reinforcement and the like, although the treatment effect of the method is outstanding, a large amount of cement-based materials, chemical materials and the like are often required to be injected into a stratum in the construction process, and certain influence is caused on the surrounding environment, and the plant root slope reinforcement, curing agent method and other methods proposed by subsequent engineering personnel have the problems that the long-term reinforcement effect cannot be ensured, the reinforcement is not uniform and the like. Therefore, how to combine the advantages of the sand slope reinforcement methods, it has become a difficult problem to be solved by engineers for a better reinforcement effect and environmental protection.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects and defects in the background technology and providing the sandy slope grouting reinforcement method based on the joint action of vegetation rhizomes and microorganisms, which is environment-friendly, good in curing effect and good in mechanical property.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a sandy slope grouting reinforcement method based on combined action of vegetation rhizome and microorganism comprises the following steps:
s1, planting a gramineous plant on a slope surface of a sandy slope, cutting off branches at the upper part of the gramineous plant after the gramineous plant naturally withers, reserving a gramineous plant stem with a certain height, and marking a first gramineous plant branch stem and a second gramineous plant branch stem;
s2, pouring a mixture of a microbial liquid and a microbial culture solution through a branch stem guide pipe of the first gramineous plant; the microbial liquid is a pasteurella bacillus liquid, and the strain is obtained by separating soil around the sandy slope;
s3, pouring a microbial nutrient solution through a second branch stem conduit of the gramineous plant, wherein the microbial nutrient solution is converged with the microbial bacterial solution and the microbial culture solution at the main stem conduit of the gramineous plant and reaches each root branch along with the conduit of the root system; the microbial nutrient solution contains urea and calcium salt.
Further, the gramineous plants of S1 are selected from gramineous plants with developed root systems and strong growth capacity, and the coverage rate is not less than 60%.
Furthermore, the stems of the gramineous plants of S1 are cut off and the branches at the upper parts are exposed to the ground surface by 5-10 cm.
Furthermore, the volume ratio of the microbial bacteria solution to the microbial culture solution in S2 is 1: 0.5-2, the concentration of the microbial bacteria solution is 3-15 mol/L, and each liter of the microbial culture solution contains 0.05-0.20 mol/L of Tris buffer solution, 5-15 g of ammonium sulfate and 10-30 g of yeast extract.
Further, the perfusion mode of S2 is to perfuse 1ml to 8ml of mixed solution of the microbial liquid and the microbial culture solution every time, perfuse once every 24 hours, and perfuse 1 to 3 times.
Further, the perfusion in S2 and S3 uses peristaltic pumps.
Further, S3 the microbial nutrient solution is prepared by mixing a urea solution and a mixed solution of calcium nitrate and calcium chloride in a volume ratio of 1: 0.5-2, wherein the urea concentration is 0.5-3 mol/L, and the calcium ion concentration in the mixed solution of calcium nitrate and calcium chloride is 0.5-1.5 mol/L.
Further, S3 the perfusion mode is to perfuse 1ml to 8ml of the microorganism nutrient solution every time, and perfuse the microorganism nutrient solution every 24 hours for 2 to 5 times.
Further, the turf greening is performed again after completion of S3.
The inventor finds out in the research that: if the microorganism liquid is injected by adopting a common injection method (a conduit method), after the conduit is inserted into the stratum, pressure difference is formed when bacteria liquid is injected into the conduit, the propagation of microorganisms is influenced by the pressure difference, nutrient solution cannot be supplied to the microorganisms for many times, and the reinforcing effect is influenced; the common grouting mode (conduit method) is adopted for grouting, microorganisms are randomly diffused to the surrounding soil through small holes of the conduit, and the accuracy of regional reinforcement is reduced. Therefore, the invention adopts the conduit in the stems and roots of the plant gramineae to carry out grouting reinforcement on the sandy slope under the combined action of the plant roots and the plant rhizomes and microorganisms so as to solve the problems.
Compared with the prior art, the invention has the beneficial effects that:
the invention takes a microbial liquid, a microbial culture solution and a microbial nutrient solution as grouting liquid to perform grouting on stems and roots of gramineous plants, the microbial liquid, the microbial culture solution and the nutrient solution are converged at a main stem conduit and reach each root branch along with the conduit of the roots, the microbes perform urea hydrolysis in the environment to separate out calcium carbonate with a cementing function, and the calcium carbonate is solidified and hardened to fill the roots to form a net-shaped reinforced anchoring structure body. The mesh-shaped reinforced anchoring structure body and the plant root system work together, shallow roots after grouting play a reinforcement role and are combined with soil particles to form a cementing effect, deep roots after grouting play an anchoring role, and the mesh-shaped reinforced anchoring structure body is similar to a prestressed anchor rod and can obviously improve the shear strength of a soil body.
Compared with the prior art, the reinforcing area is only in the root system distribution area, so that the reinforcing area is more accurate and effective. In addition, the peristaltic pump is adopted to inject the microbial bacteria liquid, so that the differential pressure caused by the microbial bacteria liquid is small, namely, the influence on the growth and the propagation of the microbes is small, and the microbial nutrient solution can be injected for multiple times, so that the microbes can be provided with a growth environment which is free from the interference of the external soil environment and has sufficient nutrient substances, and the growth and the propagation of the microbes are facilitated, and the generation of calcium carbonate is further facilitated. Compared with the traditional vegetation slope protection, the decay speed of the root system after grouting is slowed down to a certain extent, the service life is prolonged, the anchoring effect is not easy to lose along with the change of seasons, and the long-term repeated process of four-season seeding is reduced. Compared with the traditional concrete reinforced side slope, the grouting liquid adopted by the invention can not cause pollution to soil and trouble to the treatment of the side slope in the later period, and can maintain water and soil to a certain extent and improve soil mass.
In conclusion, the sandy slope grouting reinforcement method based on the combined action of the vegetation roots and the microorganisms is low in engineering cost, environment-friendly, good in solidification effect and good in mechanical property. The method can obviously improve the overall stability of the sandy side slope, and further achieve the purpose of side slope disaster prevention and control.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic illustration of the root system grouting of the present invention. Wherein: 1. a first graminaceous plant shoot; 2. a second graminaceous plant shoot; 3. a first gramineous plant branch stem conduit; 4. a second gramineous plant branch stem conduit; 5. root systems of gramineous plants; 6. leaves of gramineous plants.
Detailed Description
In order to facilitate understanding of the invention, the invention will be described more fully and in detail with reference to the accompanying drawings and preferred embodiments, but the scope of the invention is not limited to the specific embodiments below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
In one embodiment of the invention, the sandy slope grouting reinforcement method based on the joint action of vegetation rhizomes and microorganisms comprises the following steps:
1) the sandy side slope is simply cleaned, and the grass with developed root system and strong growth capability, such as vetiver grass, is sown and planted on the slope surface of the side slope. The vetiver grass has developed root system and persistent vitality, and can survive in sandy soil environment, so the vetiver grass can be used for improving the sandy soil environment. The coverage rate of the gramineous plants on the slope surface of the side slope is not less than 60 percent, and preferably 60 to 80 percent.
2) Original Papanicolaou bacilli are separated from soil around the sandy slope and are placed in a laboratory for culture. Compared with the pasteurella extracted from other areas, the pasteurella extracted from the surrounding soil can be more suitable for the local environment and climate, and has stronger survival and reproductive capacity. The bacillus pasteurianus is adopted because the bacteria are non-toxic and harmless, widely exist in soil or water environment, and have stronger biological activity in severe soil environments such as acid-base and high-salt environments.
3) After the grass plants on the slope surface of the sandy slope naturally wither, cutting off branches and trunks on the upper part of the grass plants, reserving grass plant stems, wherein the grass plant stems are exposed to the ground surface by 5 cm-10 cm; the first and second crop branches 1 and 2 are marked as shown in fig. 1.
4) Preparing a mixed solution of a microbial liquid and a microbial culture solution: the bacteria liquid is pasteurella bacillus liquid, the bacteria is derived from the pasteurella bacillus obtained in the step 2), and the culture liquid is a mixture of Tris buffer solution, ammonium sulfate and yeast extract. Preparing a microbial nutrient solution: the microorganism nutrient solution is a mixture of urea solution and mixed solution of calcium nitrate and calcium chloride.
In a specific embodiment, the specific implementation manner of step 4) is:
4.1) sufficiently mixing the microbial strain solution and the microbial culture solution by using a portable stirrer according to the volume ratio of 1: 0.5-2; the concentration of the microbial liquid is 3-15 mol/L; the microbial culture solution contains 0.05 mol/L-0.20 mol/L Tris buffer solution, 5-15 g ammonium sulfate and 10-30 g yeast extract per liter;
4.2) fully mixing the urea solution and the mixed solution of calcium nitrate and calcium chloride by using a portable stirrer according to the volume ratio of 1: 0.5-2; the concentration of the urea is 0.5-3 mol/L; the concentration of calcium ions in the mixed solution of calcium nitrate and calcium chloride is 0.5-1.5 mol/L.
5) Injecting the mixture of the microbial liquid and the microbial culture liquid prepared in the step 4) into a root system 5 of the gramineous plant through the first gramineous plant branch stem conduit 3 of the first gramineous plant branch stem 1 reserved in the step 3).
In a specific embodiment, the specific implementation manner of step 5) is: under the condition of the ambient temperature of 15-35 ℃ (preferably 25 ℃), a peristaltic pump is adopted to fill the root system 5 of the gramineous plant through the first gramineous plant branch stem conduit 3 of the first gramineous plant branch stem 1; the perfusion mode is that 1ml to 8ml of mixed solution of the microbial bacteria solution and the microbial culture solution is perfused every time, and the perfusion is carried out once every 24 hours for 1 to 3 times; and when the environmental temperature is lower than 15 ℃, a greenhouse is built in the grouting area to be heated.
6) Injecting the nutrient solution prepared in the step 4) into the root system 5 of the gramineous plant through the second gramineous plant branch and stem conduit 4 of the second gramineous plant branch and stem 2 reserved in the step 3).
In a specific embodiment, the specific implementation manner of step 6) is: under the condition of the ambient temperature of 15-35 ℃, a peristaltic pump is adopted to fill the root system 5 of the gramineous plant through the second gramineous plant branch stem conduit 4 of the second gramineous plant branch stem 2; the filling mode is that 1ml to 8ml of mixed solution of urea calcium nitrate and calcium chloride is filled every 24 hours for 2 to 5 times.
The invention adopts different conduits to respectively perfuse the bacteria liquid, the culture solution and the nutrient solution and then converge. If the same duct is adopted to inject the bacteria liquid and the nutrient solution, the microorganisms attached to the duct wall mineralize the sediment calcium carbonate by the nutrient solution, and the calcium carbonate is attached to the duct wall. This continues to cause a reduction in the area of the catheter orifice and even blockage of the catheter, affecting the speed and effectiveness of the injection. Therefore, different catheters are adopted to respectively inject the bacteria liquid and the nutrient solution, so that the growth and propagation conditions of microorganisms are ensured, the smooth circulation of the injection in the catheter is also ensured, and the target can be reached more conveniently.
7) And (5) carrying out turf greening again. And grass seeds are selected from herbaceous plants which are perennial, green throughout the year and strong in rhizome spreading capacity, such as bermudagrass.
The reinforcement principle of the invention is that microbial fluid, culture fluid and nutrient fluid are injected into plant stems and root systems, the microorganisms mineralize and precipitate calcium carbonate in the root systems, and the calcium carbonate is solidified and hardened to form a reticular reinforcement anchoring structure body. The mesh-shaped reinforced anchoring structure body works together with a plant root system, shallow roots after grouting play a reinforcement role and are combined with soil particles to form a cementing effect, deep roots after grouting play an anchoring role, the mesh-shaped reinforced anchoring structure body is similar to a prestressed anchor rod, the prestressed anchoring role is played, and the shear strength of a soil body can be obviously improved. In addition, the rotting speed of the root system after grouting is reduced to a certain extent, the service life is prolonged, and the slope is not easy to damage due to the fact that the anchoring effect is lost along with the change of seasons.
The invention adopts calcium carbonate generated by microbial mineralization to carry out grouting reinforcement on the root system, and the soil inherently contains calcium carbonate, so the calcium carbonate generated by the mineralization basically does not generate adverse effect on the soil body, and can neutralize the acidity of the soil and improve the soil fertility.
Example 1:
planting and constructing the gramineous plants:
sowing the seeds of the vetiver grass on the slope surface of the side slope, wherein the coverage rate of the vetiver grass on the slope surface of the side slope is 60-80 percent; after the vetiver grass is mature, a first branch stem 1 of the gramineous plant, a first branch stem conduit 3 of the gramineous plant, a second branch stem 2 of the gramineous plant, a second branch stem conduit 4 of the gramineous plant and leaves 6 of the gramineous plant are distributed on the ground surface; root systems 5 of the gramineous plants are distributed underground;
after the vetiver grass naturally withers, the upper branches of the plants are removed, and the stems of the gramineous plants are reserved; the stalk of the gramineous plant is 5 cm-10 cm exposed on the ground surface.
Preparing a mixed solution of a microbial liquid and a microbial culture solution and a microbial nutrient solution:
fully mixing the microbial strain solution and the microbial culture solution by using a portable stirrer according to the volume ratio of 1: 1; the concentration of the microbial liquid is 10 mol/L; the microbial culture solution contains 0.1mol/L Tris buffer solution, 10g ammonium sulfate and 20g yeast extract per liter;
fully mixing the urea solution and the mixed solution of calcium nitrate and calcium chloride according to the volume ratio of 1: 0.5-2 by using a portable stirrer; the concentration of urea is 2 mol/L; the concentration of calcium ions in the mixed solution of calcium nitrate and calcium chloride is 1 mol/L; the mixed solution of the urea solution and the calcium nitrate and calcium chloride is the microorganism nutrient solution.
And (3) carrying out microbial grouting construction on the plant root system:
under the condition of the ambient temperature of 25 ℃, a peristaltic pump is adopted to fill the root system 5 of the gramineous plant through the first gramineous plant branch stem conduit 3 of the first gramineous plant branch stem 1; the perfusion mode is that 4ml of mixed solution of the microbial bacteria solution and the microbial culture solution is perfused every 24 hours for 2 times.
Under the condition of the ambient temperature of 25 ℃, a peristaltic pump is adopted to fill the root system 5 of the gramineous plant through the second branch stem conduit 4 of the second branch stem 2 of the gramineous plant; the perfusion mode is that 4ml of mixed solution of urea calcium nitrate and calcium chloride is perfused every 24 hours for 4 times.
And re-compounding grass with bermudagrass seed. And (3) comparing the ecological conditions of the slope after continuous rainfall, wherein the slope does not have obvious phenomena of landslide, erosion or local collapse and the like under the action of the continuous rainfall, and the method is shown to be capable of effectively reinforcing the slope.
The foregoing is considered as illustrative of the preferred embodiments of the invention and is not to be construed as limiting the invention in any way. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention should fall within the protection scope of the technical scheme of the present invention, unless the technical spirit of the present invention departs from the content of the technical scheme of the present invention.
Claims (9)
1. A sandy slope grouting reinforcement method based on the combined action of vegetation rhizome and microorganism is characterized by comprising the following steps:
s1, planting a gramineous plant on a slope surface of a sandy slope, cutting off branches at the upper part of the gramineous plant after the gramineous plant naturally withers, reserving a gramineous plant stem with a certain height, and marking a first gramineous plant branch stem and a second gramineous plant branch stem;
s2, pouring a mixture of a microbial liquid and a microbial culture solution through a branch stem guide pipe of the first gramineous plant; the microbial liquid is a pasteurella bacillus liquid, and the strain is obtained by separating soil around the sandy slope;
s3, pouring a microbial nutrient solution through a second branch stem conduit of the gramineous plant, wherein the microbial nutrient solution is converged with the microbial bacterial solution and the microbial culture solution at the main stem conduit of the gramineous plant and reaches each root branch along with the conduit of the root system; the microbial nutrient solution contains urea and calcium salt.
2. The sandy slope grouting reinforcement method based on combined action of vegetation rhizomes and microorganisms as claimed in claim 1, wherein the gramineous plants of S1 are selected from gramineous plants with developed plant root systems and strong growth capability, and the coverage rate is not less than 60%.
3. The sandy slope grouting reinforcement method based on the joint action of vegetation rhizomes and microorganisms as claimed in claim 1 or 2, wherein the stems of the gramineous plants at S1 are cut to remove the upper branches and expose the earth surface for 5-10 cm.
4. The sandy slope grouting reinforcement method based on vegetation rhizome and microorganism combined action of claim 1, wherein S2 the volume ratio of the microorganism liquid to the microorganism culture liquid is 1: 0.5-2, the concentration of the microorganism liquid is 3-15 mol/L, and the microorganism culture liquid contains 0.05-0.20 mol/L of Tris buffer solution, 5-15 g of ammonium sulfate and 10-30 g of yeast extract per liter.
5. The sandy slope grouting reinforcement method based on combined action of vegetation rhizomes and microorganisms according to claim 1 or 4, wherein S2 is perfused in a manner of perfusing a mixed solution of 1-8 ml of microorganism bacterium solution and microorganism culture solution every time, perfusing once every 24 hours, and perfusing for 1-3 times.
6. The sandy slope grouting reinforcement method based on vegetation rhizome and microorganism combined action according to claim 1 or 4, characterized in that peristaltic pumps are adopted for the perfusion of S2 and S3.
7. The sandy slope grouting reinforcement method based on vegetation rhizome and microorganism combined action of claim 1, wherein S3 the microorganism nutrient solution is prepared by mixing a urea solution and a mixed solution of calcium nitrate and calcium chloride in a volume ratio of 1: 0.5-2, the urea concentration is 0.5-3 mol/L, and the calcium ion concentration in the mixed solution of calcium nitrate and calcium chloride is 0.5-1.5 mol/L.
8. The sandy slope grouting reinforcement method based on combined action of vegetation rhizomes and microorganisms according to claim 1 or 7, wherein S3 is poured in a mode that 1ml to 8ml of microorganism nutrient solution is poured each time, and the microorganism nutrient solution is poured once every 24 hours for 2 to 5 times.
9. The method for grouting reinforcement of sandy slopes based on combined action of vegetation rhizomes and microorganisms according to claim 1, 2, 4 or 7, wherein turf greening is performed again after completion of S3.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113373958A (en) * | 2021-07-21 | 2021-09-10 | 苏州金螳螂园林绿化景观有限公司 | Microbial curing liquid for sandy side slope and sandy side slope treatment method |
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| CN109518703A (en) * | 2019-01-15 | 2019-03-26 | 内蒙古工业大学 | A kind of administering method of the cementing treatment fluid of microbial mineralization and chiltern side slope |
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| US20170181392A1 (en) * | 2013-10-04 | 2017-06-29 | Charles E. Ankner | Method of Improving the Growth and Production Output of Plants of the Family Cannabaceae sensu stricto |
| CN107023024A (en) * | 2017-04-25 | 2017-08-08 | 湖北工业大学 | A kind of road slope reinforcing and processing method based on axis and root system slip casting |
| CN109518678A (en) * | 2018-11-02 | 2019-03-26 | 东南大学 | A kind of method of plant mucilage-microbial association soil mass consolidation |
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| CN113373958A (en) * | 2021-07-21 | 2021-09-10 | 苏州金螳螂园林绿化景观有限公司 | Microbial curing liquid for sandy side slope and sandy side slope treatment method |
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