CN108812686A - A kind of microbial source antiviral composition, preparation and its application of the GP-1 containing glycoprotein - Google Patents
A kind of microbial source antiviral composition, preparation and its application of the GP-1 containing glycoprotein Download PDFInfo
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- CN108812686A CN108812686A CN201810399564.5A CN201810399564A CN108812686A CN 108812686 A CN108812686 A CN 108812686A CN 201810399564 A CN201810399564 A CN 201810399564A CN 108812686 A CN108812686 A CN 108812686A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Abstract
The invention discloses microbial source antiviral composition, preparation and its applications of a kind of GP-1 containing glycoprotein, the microbial source antiviral composition of the GP-1 containing glycoprotein, the composition includes jamaicin, further includes the metabolin of glycoprotein GP-1 and/Kanas Lake streptomycete (Streptomyces kanasensis ZX01).The antiviral composition all has good control efficiency to various plants virosis such as tobacco mosaic virus disease (Tobacco mosaic virus), Cucumber Mosaic Virus (Cucumber mosaic virus), tomato yellow leaf curl virus sick (Tomato yellow leaf curl virus), potato virus disease (Potato virus X/Y), rice dwarf virus disease viral diseases (Rice dwarf virus), its mode of action is novel, conventional pesticide can be effectively relieved, bring resistance problem is used for a long time, moreover it is possible to improve disease resistance of plant.Antivirotic of the invention has the characteristics that efficient, wide spectrum, low cost, and conventional application method can be used, free from environmental pollution.
Description
Technical field
The present invention relates to Microbial Metabolites against Plant Virus Diseases matter, pesticidal preparations and its applications, in particular to a kind of containing sugar
Microbial source antiviral composition, preparation and its application of Protein G P-1.
Background technique
Universal, prevention and treatment difficulty occurs for the viroses of plant, seriously endangers agricultural production, and worldwide cause huge
Big economic loss.Microorganism is the valuable source library of anti-plant virus agent research and development, screens and separates from microbial metabolic products
Metabolites against Plant Virus Diseases matter is always the research and development focus of domestic and foreign scholars.
Kanas Lake streptomycete (Streptomyces kanasensis ZX01) is be isolated from Xinjiang Kanas Lake lakeside one
The novel actinomyces of kind, metabolite have Antiphytoviral and Fungicidally active, and wherein glycoprotein GP-1 is main activity
Ingredient has the effect of antiviral, induction disease resistance of plant and volume increase, to plant safety, is not likely to produce drug resistance, can be used as
The active constituent of the favourable agents of agricultural disease prevention and treatment.
Summary of the invention
The purpose of the present invention is to provide the microbial source antiviral composition of GP-1 containing glycoprotein a kind of, preparation and its answer
With under the conditions of certain mixing ratio, showing unpredictable increasing after glycoprotein GP-1 of the invention is mixed with jamaicin
Imitate effect.
In order to realize that above-mentioned task, the present invention take following technical solution to be achieved：
A kind of microbial source antiviral composition of the GP-1 containing glycoprotein, the composition includes jamaicin, further includes sugared egg
The metabolin of white GP-1 and/Kanas Lake streptomycete (Streptomyces kanasensis ZX01).
Optionally, the preparation of the metabolin of the Kanas Lake streptomycete (Streptomyces kanasensis ZX01)
Including：
Kanas Lake streptomycete (Streptomyces kanasensis ZX01) is in the 28 DEG C of fermentation trainings of millet fermentation medium
It is filtered after supporting 7 days, filtrate is spray-dried to obtain powder to obtain the final product；
The millet culture medium is：Millet 10g, glucose 10g, peptone 3g, NaCl2.5g, CaCO30.2g, water
1L, pH 7.2~7.4.
Optionally, the glycoprotein GP-1 is isolated from Kanas Lake streptomycete (Streptomyces kanasensis
ZX01 metabolin).
A kind of microbial source anti-virus formulation of the GP-1 containing glycoprotein, the preparation include effective component and auxiliary agent, institute
The effective component stated is the microbial source antiviral composition of the GP-1 containing glycoprotein.
Optionally, the dosage form is soluble liquid, aqua or wettable powder.
Optionally, soluble liquid is prepared by weight percentage by following raw material：The microbe metabolite of GP-1 containing glycoprotein：
20%~40%, jamaicin 0~10%, surfactant：5%~15%, antifreezing agent：5%, surplus is wood vinegar, raw material
The sum of weight percent is 100%.
Optionally, the microbe metabolite of GP-1 containing glycoprotein：20%~40%, jamaicin：0~10%；Surfactant：
5%~15%, antifreezing agent：5%, surplus is water, and the sum of weight percent of raw material is 100%.
Optionally, wettable powder preparation proportion：The microbe metabolite of GP-1 containing glycoprotein：20%~40%, jamaicin：
0~10%, surfactant：5%~15%, remaining is filler, and the sum of weight percent of raw material is 100%.
The microbial source antiviral composition of the GP-1 containing glycoprotein is used to prepare prevention and treatment plant pathogenic fungi, bacterium
And/or the application of the pesticide and/or fertilizer of plant disease caused by virus.
Optionally, the fungus-caused plant disease includes graw mold of tomato, the late blight of potato, wheat scab
And apple anthracnose；
Plant disease caused by bacterium includes soft rot of cabbage and bacterial blight of rice；
The virus includes tobacco mosaic virus (TMV), cucumber mosaic virus, corium solani, tomato yellow leaf curl
Viral disease and fractilinea oryzae.
Advantages of the present invention includes：
The microbial source antiviral composition of the GP-1 containing glycoprotein of the invention is that a kind of novel environment-friendly microbial source is anti-
Viral agent is suitable for prevention and treatment tobacco mosaic virus disease, tomato yellow leaf curl virus disease, Cucumber Mosaic Virus etc..Its effect side
Formula is novel, can effectively alleviate conventional pesticide and bring resistance problem is used for a long time, meet the trend of agricultural and Agrochemicals.It is former
Expect simple and easy to get, significant effect, it is easy to industrialized production, have it is at low cost, to environment, safety of human and livestock, be not likely to produce drug resistance
The features such as, and conventional application method can be used.Especially the present invention has studied glycoprotein GP-1 is mixed with jamaicin after, one
Under the conditions of fixed mixing ratio, unpredictable synergistic effect is shown.
Specific embodiment
Kanas Lake streptomycete (Streptomyces kanasensis ZX01) in Han LR, Zhang GQ, Miao GP,
Zhang X*,Feng JT.Streptomyces kanasensis sp nov.,an AntiviralGlycoprotein
Producing Actinomycete Isolated from Forest Soil Around Kanas Lake of
China.CURRENTMICROBIOLOGY,2015,71(6):It is recorded in 627-631. document, actinomyces ZX01 is from Xinjiang noise made in coughing or vomiting
Receive isolated one plant of actinomyces in the soil of this lakeside woods.Sort out by strain morphology feature, ZX01 can be belonged to
The pink spore monoid of streptomycete.ZX01 bacterial strain is identified with polyphase sort identification method combination DNA homology, by streptomycete
It is accredited as 1 novel species, is named as Streptomyces kanasensis ZX01.Kanas Lake strepto- as mentioned in the present invention
Bacterium (Streptomyces kanasensis ZX01).
Glycoprotein GP-1 is isolated from Kanas Lake streptomycete ZX01 metabolin.The glycoprotein molecule amount is about
8500Da, polysaccharide and protein content are respectively 40.23% and 54.36%；Protein part is made of 15 kinds of amino acid, wherein acid
Amino acid Asp and Glu content is higher (15.15% and 12.63%), lower (0.62% He of basic amino acid Arg and Lys content
4.88%)；Saccharide portion is made of 6 kinds of monosaccharide, is mannose respectively, xylose, arabinose, glucose, Glucosamine and
Galactolipin (0.38:0.14:0.40:2.62:0.10:1.00), by mass spectral analysis, GP-1 is the more novel sugar of a structure
Albumen.Extracting method is referring to the anti-TMV active material separation of Zhang Guoqiang Kanas Lake streptomycete ZX01 and global regulation gene nsdA resistance
Disconnected to study [D] Xibei Univ. of Agricultural & Forest Science & Technology, the method in 2016. carries out the acquisition of glycoprotein GP-1.
Jamaicin is also known as berberine, is a kind of quartermary ammonium alkaloids separated from Chinese Drug Rhizomes of Coptis, is the main of coptis antibacterial
Effective component.For yellow needles, bitter.Distributed more widely in plant kingdom, about 4 sections 10, which belong to interior discovery, this product
In the presence of.Clinical application is mainly the hydrochloride and sulfate of this product.The current synthetic method mass production in China.It can pass through on the market
Purchase obtains.
The microbial source antiviral composition of the GP-1 containing glycoprotein of the invention is used to prepare prevention and treatment plant pathogenic fungi, thin
The application of the pesticide and/or fertilizer of plant disease caused by bacterium and/or virus.For example fungus-caused plant disease includes tomato
Gray mold, the late blight of potato, wheat scab and apple anthracnose；Plant disease caused by bacterium include soft rot of cabbage and
Bacterial blight of rice；Virus includes tobacco mosaic virus (TMV), cucumber mosaic virus, corium solani, tomato yellow leaf curl
Viral disease and fractilinea oryzae.
The present invention is described in further detail for test and embodiment below in conjunction with inventor.
One, the acquisition of the microbe metabolite of the GP-1 containing glycoprotein
Kanas Lake streptomycete (Streptomyces kanasensis ZX01) 28 DEG C of fermentations in millet fermentation medium
Culture is filtered after 7 days, and filtrate is spray-dried, and obtained powder is the microbe metabolite of the GP-1 containing glycoprotein.This
A metabolin is the mixture containing glycoprotein GP-1, and other components also have certain drug effect, but main effect is glycoprotein GP-
1.It is isolated by the method for tracking activity that glycoprotein GP-1 is this mixture, by tracking activity and a large amount of fraction sections
Active bio measurement, very clear glycoprotein GP-1 play main drug effect.Referring to the anti-TMV of Zhang Guoqiang Kanas Lake streptomycete ZX01
Active material separation and global regulation gene nsdA, which are blocked, studies [D] Xibei Univ. of Agricultural & Forest Science & Technology, and 2016
Two, the preparation of the antiviral environmentally friendly preparation of the microbial source of the GP-1 containing glycoprotein
The materials such as microbe metabolite, jamaicin, wood vinegar, surfactant by the GP-1 containing glycoprotein are configured to solvable
Property liquor, aqua or wettable powder, for prevent and treat have the viroses of plant caused by plant virus.Each original in above-mentioned various dosage forms
The weight percent of material matter is referring to following table：
Above-mentioned surfactant used is one or more of mixing of all kinds of surface reactive materials commonly used in the art
Object；
Filler used be one of white carbon black, bentonite, diatomite or the known substance of this field staff or
Several mixtures.
Antifreezing agent used is ethylene glycol or propylene glycol.
Three, glycoprotein antivirotic prevents and treats tobacco mosaic virus disease synergistic effect result
The measurement of control effect testing (1) TMV inactivation in vitro.The external passivation of TMV is made with half leaf withered spot method test agents
With.Specific method:The left half leaf inoculation medicament of 4~6 vigorous leaf phase Nicotiana glutinosas of healthy growth is chosen to mix with the isometric of virus
Liquid, isometric mixed liquor (control) of right half leaf inoculation distilled water and virus, is repeated 3 times.Withered spot number is calculated after 5~7d of inoculation.
Withered spot inhibiting rate calculation formula：Withered spot inhibiting rate (%)=(control withered spot number-processing withered spot number)/control withered spot number
× l00%.
(2) to the measurement of TMV First aggression and duplication proliferation function.Equally tested with half leaf withered spot method.Specific method:
The vigorous 4-6 leaf phase Nicotiana glutinosa of healthy growth is chosen, first in left half leaf coating interval, one timing of test plant in First aggression test
Between after, then full leaf virus inoculation；First full leaf virus inoculation in proliferation test is replicated, after separated in time, then in test plant
Half leaf coating of a left side.Plant extraction liquid concentration is 50 times of liquid, and lentinan is compareed as medicament, and concentration is 400 times of liquid, and virus connects
Kind concentration is 10mg/mL.Each processing is repeated 3 times, and withered spot number is calculated after 5~7d, calculates withered spot inhibiting rate according to the above method.
(3) between active material synergistic effect measuring method.It is calculated using Y.P.Sun and E.R.Johnson method multiple
The co-toxicity coefficient of ingredients, and compounding agent synergistic effect is evaluated according to co-toxicity coefficient size, and determine optimum proportioning.
Toxicity index (TI)=standard antiseptic agent EC50/ reagent agent EC50 × 100；
Compound preparation actual toxicity index (ATI)=standard agent EC50/ mixture EC50；
TI × PB of compound preparation agent theoretical toxicity index (TTI)=single dose A TI × PA+ single dose B, (PA and PB difference
It is the percentage composition of single dose A and single dose B effective component in mixture)；
The theoretical toxicity index TTI of compound preparation co-toxicity coefficient (CTC)=mixture actual toxicity index ATI/ mixture；
Synergistic effect judgement：CTC>It is synergistic effect when 120；CTC<It is antagonism when 80；It is when 80≤CTC≤120
Summation action.
The setting of 1 reagent agent experimental concentration of table
According to the analysis of related data, each test medicine is calculated to the withered spot inhibiting rate of TMV.And probability is converted by its value
It is worth (y), converts corresponding logarithm (x) for drug concentration, each medicament can be found out to the virulence of TMV according to correlation formula
Regression curve equation：Y=a+bx and EC50 value and related coefficient (r).
Inhibitory activity and compound synergic result of the two kinds of active components to TMV virosis:
Inhibitory activity of 2 two kinds of active components of table to TMV virosis
Note:Significant difference between same column difference lowercase expression processing.
3 different proportion of table combines the co-toxicity coefficient of anti-TMV
2 table 3 of table shows that glycoprotein and jamaicin have good antiviral activity, and glycoprotein is being compounded with jamaicin
When, its ratio be 1：1 and 3：When 7, co-toxicity coefficient is greater than 120, shows as synergistic effect.
Test example
It should be noted that embodiments given below is only preferable example, the present invention is not limited to these Examples,
Satisfactory antivirotic can be obtained in the scope of the present invention.
Embodiment 1：The preparation of the antiviral soluble liquid of 0.05% glycoprotein, 0.2% jamaicin
At 20~25 DEG C, 5 parts of microbe metabolite, 0.2 part of jamaicin, 5 parts of isopropyls for containing 1% glycoprotein GP-1 are taken
After alcohol, 5 parts of dodecyl sodium sulfonate calcium, 5 parts of styrenated phenol polyoxyethylene ether and 79.8 parts of wood vinegar, in a kettle to stir speed
The speed of 800~1000 revs/min of degree stirs 10~30 minutes, can be obtained anti-containing 0.05 glycoprotein, 0.2% jamaicin
Viral soluble liquid.
Embodiment 2：The preparation of the antiviral aqua of 0.1% glycoprotein, 0.2% jamaicin
At 20~25 DEG C, 10 parts of microbe metabolite, 0.2 part of jamaicin, 2 parts of isopropyls for containing 1% glycoprotein GP-1 are taken
After alcohol, 5 parts of dodecyl sodium sulfonate calcium and 82.8 parts of water, stirred in a kettle with 800~1000 revs/min of mixing speed of speed
It mixes 10~30 minutes, can be obtained containing the antiviral aqua of 0.1 glycoprotein, 0.2% jamaicin.
Embodiment 3：The preparation of the antiviral wettable powder of 0.2% glycoprotein, 0.2% jamaicin
At 20~25 DEG C, 20 parts of the microbe metabolite powder for containing 1% glycoprotein GP-1,15 parts of sodium lignin sulfonates, 5 are taken
Part ethoxylated dodecyl alcohol and 60 parts of white carbon blacks, addition are mixed well into mixing machine to get 0.2% glycoprotein 0.2%
The antiviral wettable powder of jamaicin.
The glycoprotein antivirotic of above-described embodiment 1-3 prevents and treats tobacco mosaic virus disease field test results：
Compounding method of preparation according to the invention, the disease-resistant virus soluble of 0.05% glycoprotein, 0.2% jamaicin of preparation
Liquor, the antiviral aqua of 0.1% glycoprotein, 0.2% jamaicin and the antiviral wettable of 0.2% glycoprotein, 0.2% jamaicin
Pulvis has carried out the test of prevention and treatment tobacco mosaic virus disease and tomato yellow leaf curl virus disease in crop field, more using 0.5% mushroom
Syrup agent is comparison medicament, and clear water is control.
Prevention and treatment tobacco mosaic virus disease test result investigation method takes the mode of whole strain investigation, marks according to classification below
Standard is classified：
0 grade：Complete stool is disease-free；
1 grade：The bright arteries and veins of lobus cardiacus or slight floral leaf；Diseased plant is downgraded without obvious.
3 grades：It is 3/4 or more of normal plant height that 1/3 blade floral leaf but indeformable or diseased plant, which are downgraded,；
5 grades：Blade floral leaf, or it is normal plant height 2/ that a small number of deformable blades or master pulse blackening or diseased plant, which are downgraded,
3-3/4
7 grades：Blade floral leaf, or deformation or the necrosis of main side arteries and veins or diseased plant downgrade the 1/2-2/3 for normal plant height；
9 grades：Complete stool blade floral leaf, it is 1/2 or more of normal plant height that severely deformed or downright bad or strain, which is downgraded,.
As a result it counts：
Disease index and control efficiency are calculated according to following formula：
Disease index=∑ (sick series × diseased plant number)/(highest disease grade × respectively handle total strain number)
Control efficiency=(control disease refer to-handles disease and refers to)/control disease refers to × 100%
Field prevention experimental result is shown in Table 4：
4 glycoprotein GP-1 antivirotic of table to the field prophylactic tria result of tobacco mosaic virus (TMV) (Henan Shengchi County, 2015
The 6-7 month in year)
Test result shows 200 times of three kinds of preparations and the hair of the good preventing tobacco mosaic virus diseases of 400 times of liquid energy
It is raw, 300 times of liquid phases of control efficiency and 0.5% lentinan comparison medicament of tobacco mosaic virus disease are worked as.
Prevention and treatment tomato yellow leaf curl virus disease test result investigation method takes the mode of whole strain investigation, according to below point
Grade standard is classified：
0 grade：It is asymptomatic；
1 grade：Yellow that top vane is slight, limb edge slightly crimp, florescence flower variation；
2 grades：Top vane moderate yellow, limb edge mild crimp fold, florescence flower variation, fruiting period yield is slight
The underproduction.
3 grades：The serious yellow of blade, curling, fold, florescence flower moderate fall off, the fruiting period yield moderate underproduction；
4 grades：Large area blade severe deformities reduce, and plant strain growth is slow, hence it is evident that downgrade, the florescence, Hua Yandu fell off, as a result
Phase yield Severe Reduction or total crop failure.
As a result it counts：
Disease index and control efficiency are calculated according to following formula：
Disease index=∑ (sick series × diseased plant number)/(highest disease grade × respectively handle total strain number)
Control efficiency=(control disease refer to-handles disease and refers to)/control disease refers to × 100%
Field prevention experimental result is shown in Table 5：
5 glycoprotein GP-1 antivirotic of table to the field prophylactic tria result of tomato yellow leaf curl virus disease (weinan,
The 6-7 month in 2014)
Test result shows 200 times of three kinds of preparations and 400 times of liquid can prevent tomato yellow leaf curl virus disease well
Generation, 0.5% lentinan comparison medicament, 300 times of liquid are superior to the control efficiency of tomato yellow leaf curl virus disease.
Claims (10)
1. the microbial source antiviral composition of GP-1 containing glycoprotein a kind of, which is characterized in that the composition includes jamaicin, also
Metabolin including glycoprotein GP-1 and/Kanas Lake streptomycete (Streptomyces kanasensis ZX01).
2. the microbial source antiviral composition of the GP-1 according to claim 1 containing glycoprotein, which is characterized in that described
The preparation of the metabolin of Kanas Lake streptomycete (Streptomyces kanasensis ZX01) includes：
Kanas Lake streptomycete (Streptomyces kanasensis ZX01) is in 28 DEG C of millet fermentation medium fermented and cultured 7 days
After filter, filtrate is spray-dried to obtain powder to obtain the final product；
The millet culture medium is：Millet 10g, glucose 10g, peptone 3g, NaCl2.5g, CaCO30.2g, water 1L, pH
7.2~7.4.
3. the microbial source antiviral composition of the GP-1 according to claim 1 containing glycoprotein, which is characterized in that described
Glycoprotein GP-1 is isolated from the metabolin of Kanas Lake streptomycete (Streptomyces kanasensis ZX01).
4. the microbial source anti-virus formulation of GP-1 containing glycoprotein a kind of, which is characterized in that the preparation includes effective component
And auxiliary agent, the effective component are the microbial source antiviral compound of any GP-1 containing glycoprotein of claim 1-3
Object.
5. the microbial source anti-virus formulation of the GP-1 according to claim 4 containing glycoprotein, which is characterized in that the system
Dosage form state is soluble liquid, aqua or wettable powder.
6. the microbial source anti-virus formulation of the GP-1 according to claim 5 containing glycoprotein, which is characterized in that soluble liquid
Agent is prepared by weight percentage by following raw material：The microbe metabolite of GP-1 containing glycoprotein：20%~40%, jamaicin 0~
10%, surfactant：5%~15%, antifreezing agent：5%, surplus is wood vinegar, and the sum of weight percent of raw material is
100%.
7. the microbial source anti-virus formulation of the GP-1 according to claim 5 containing glycoprotein, which is characterized in that aqua preparation
Proportion is：The microbe metabolite of GP-1 containing glycoprotein：20%~40%, jamaicin：0~10%；Surfactant：5%~
15%, antifreezing agent：5%, surplus is water, and the sum of weight percent of raw material is 100%.
8. the microbial source anti-virus formulation of the GP-1 according to claim 5 containing glycoprotein, which is characterized in that wettable powder
Agent preparation proportion：The microbe metabolite of GP-1 containing glycoprotein：20%~40%, jamaicin：0~10%, surfactant：5%
~15%, remaining is filler, and the sum of weight percent of raw material is 100%.
9. the microbial source antiviral composition of the GP-1 containing glycoprotein described in claim 1-3 is used to prepare prevention and treatment pathogenic
The application of the pesticide and/or fertilizer of plant disease caused by fungi, bacterium and/or virus.
10. application according to claim 9, which is characterized in that the fungus-caused plant disease includes tomato ash
Mildew, the late blight of potato, wheat scab and apple anthracnose；
Plant disease caused by bacterium includes soft rot of cabbage and bacterial blight of rice；
The virus includes tobacco mosaic virus (TMV), cucumber mosaic virus, corium solani, tomato yellow leaf curl virus disease
And fractilinea oryzae.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662737A (en) * | 2019-11-28 | 2020-09-15 | 中国海洋大学 | Method for reducing antibiotic resistance genes in soil by using wood vinegar and application |
| CN116813611A (en) * | 2023-08-24 | 2023-09-29 | 云南省农业科学院生物技术与种质资源研究所 | Preparation method and application of isoquinoline alkaloid |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156603A (en) * | 2007-05-11 | 2008-04-09 | 山东省农业科学院植物保护研究所 | compound against plant virus |
| CN102337239A (en) * | 2011-10-08 | 2012-02-01 | 杨凌农科大无公害农药研究服务中心 | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases |
| CN105076163A (en) * | 2015-08-26 | 2015-11-25 | 广西大学 | New application of berberine serving as agricultural fungicide |
-
2018
- 2018-04-28 CN CN201810399564.5A patent/CN108812686A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156603A (en) * | 2007-05-11 | 2008-04-09 | 山东省农业科学院植物保护研究所 | compound against plant virus |
| CN102337239A (en) * | 2011-10-08 | 2012-02-01 | 杨凌农科大无公害农药研究服务中心 | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases |
| CN105076163A (en) * | 2015-08-26 | 2015-11-25 | 广西大学 | New application of berberine serving as agricultural fungicide |
Non-Patent Citations (1)
| Title |
|---|
| 张国强: "喀纳斯链霉菌ZX01抗TMV活性物质分离及全局调控基因nsdA阻断研究", 《西北农林科技大学2016届博士学位论文》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111662737A (en) * | 2019-11-28 | 2020-09-15 | 中国海洋大学 | Method for reducing antibiotic resistance genes in soil by using wood vinegar and application |
| CN111662737B (en) * | 2019-11-28 | 2021-09-07 | 中国海洋大学 | Application of pyroligneous liquor medium-temperature-section rectification component in reduction of antibiotic resistance gene contaminated soil |
| CN116813611A (en) * | 2023-08-24 | 2023-09-29 | 云南省农业科学院生物技术与种质资源研究所 | Preparation method and application of isoquinoline alkaloid |
| CN116813611B (en) * | 2023-08-24 | 2023-12-01 | 云南省农业科学院生物技术与种质资源研究所 | Preparation method and application of isoquinoline alkaloid |
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Application publication date: 20181116 |
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