CN102337239A - Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases - Google Patents
Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases Download PDFInfo
- Publication number
- CN102337239A CN102337239A CN2011103017369A CN201110301736A CN102337239A CN 102337239 A CN102337239 A CN 102337239A CN 2011103017369 A CN2011103017369 A CN 2011103017369A CN 201110301736 A CN201110301736 A CN 201110301736A CN 102337239 A CN102337239 A CN 102337239A
- Authority
- CN
- China
- Prior art keywords
- streptomyces
- strain
- virus
- bacterial strain
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a Streptomyces sp. ZX01 strain. A fermentation product obtained through fermentation culture of the Streptomyces sp. ZX01 strain can be used for preventing and controlling tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and barley yellow dwarf virus (BYDV) and various other plant virus diseases. The Streptomyces sp. ZX01 strain with the accession number of CGMCC No.4893 is preserved in China General Microbiological Culture Collection Center in May 24, 2011.
Description
Technical field
The invention belongs to mikrobe and Application Areas thereof, the application that is specifically related to a kind of streptomyces strain and is used to prepare the control of plant virosis preparation.
Background technology
Plant virus is a kind ofly to contaminate active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind found of the whole world reaches 1122 at present.The caused Plant diseases of plant virus have the title of " plant cancer ", and its hazard rating to farm crop is only second to the mycosis original, and almost every kind of farm crop all receive the harm of 2～3 kinds of viruses.Global agriculture prodn in viroses of plant serious harm, it is reported, whole world every year, due to illness the plant loss that causes of poison harm reached 60,000,000,000 dollars unexpectedly, wherein only the financial loss in an every year of food crop just up to 20,000,000,000 dollars.
China has a lot of important viruses to spread all over national major production areas because of the loss that plant virus harm causes is difficult to counting every year.Like 70～eighties of twentieth century, north China is popular because of wheat class BYDV, bushy stunt, the general underproduction 20%～30%, and serious reaches 60%, even total crop failure.In recent years, cucumber mosaic virus, tobacco mosaic virus(TMV) and blister beetle mosaic virus cause many vegetables underproduction even total crop failure.Therefore, the control of crop virosis evil all is a great problem of puzzlement agriculture prodn all the time.
Because the special born of the same parents' endoparasitism property of plant virus combines the propagation of virus self and host's metabolism together; The immunity system of plant lack of complete in addition, virus be in case contaminate plant, just can survival in indefinitely in vegetable cell, and until host's death.Therefore, make that the control of the viroses of plant is more difficult with respect to the control of pests such as sick (fungi, bacterium), worm, grass.In addition, the plant virus kind is many, and the route of transmission is wide and exist and change into new virus and be incorporated into the genomic risk of host, has also strengthened the difficulty of viroses of plant controls.At present, except immunological species, still untappedly go out selectivity and the desirable preparation of or low toxicity nontoxic that virus is had height to the host.The anti-virus formulation of present stage exploitation is mostly to put prevention first; And how undesirable drug effect is; Its inhibiting rate generally has only 30%～60% under best working concentration; It is more single to add pharmacy variety, makes virus produce resistance to these medicaments easily, and the Antiphytoviral new drug of therefore seeking efficient, wide spectrum, safety is the difficult point and the focus of current pesticide research.
Summary of the invention
The objective of the invention is to, a streptomyces strain (Streptomyces sp.) ZX01 (hereinafter to be referred as bacterial strain ZX01) is provided, and can be used in the application of preparation anti-plant viral disease preparation through this bacterial strain of evidence ZX01.
Bacterial strain ZX01 of the present invention submits China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on May 24th, 2011, and preserving number is CGMCC No.4893.
Through measuring, the 16SrDNA sequence of this bacterial strain ZX01 is as follows:
tgcagtcgaa?cgatgaaccc?acttcggtgg?gggattagtg?gcgaacgggt?gagtaacacg?60
tgggcaatct?gcccttcact?ctgggacaag?ccctggaaac?ggggtctaat?accggatacg?120
accacttcag?gcatctgatg?gtggtggaaa?gctccggcgg?tgaaggatga?gcccgcggcc?180
tatcagcttg?ttggtggggt?aatggcccac?caaggcgacg?acgggtagcc?ggcctgagag?240
ggcgaccggc?cacactggga?ctgagacacg?gcccagactc?ctacgggagg?cagcagtggg?300
gaatattgca?caatgggcga?aagcctgatg?cagcgacgcc?gcgtgaggga?tgacggcctt?360
cgggttgtaa?acctctttca?gcagggaaga?agcgaaagtg?acggtacctg?cagaagaagc?420
gccggctaac?tacgtgccag?cagccgcggt?aatacgtagg?gcgcaagcgt?tgtccggaat?480
tattgggcgt?aaagagctcg?taggcggctt?gtcacgtcgg?atgtgaaagc?ccggggctta?540
accccgggtc?tgcattcgat?acgggcaggc?tagagtgtgg?taggggagat?cggaattcct?600
ggtgtagcgg?tgaaatgcgc?agatatcagg?aggaacaccg?gtggcgaagg?cggatctctg?660
ggccattact?gacgctgagg?agcgaaagcg?tggggagcga?acaggattag?ataccctggt?720
agtccacgcc?gtaaacgttg?ggaactaggt?gttggcgaca?ttccacgtcg?tcggtgccgc?780
agctaacgca?ttaagttccc?cgcctgggga?gtacggccgc?aaggctaaaa?ctcaaaggaa?840
ttgacggggg?cccgcacaag?cagcggagca?tgtggcttaa?ttcgacgcaa?cgcgaagaac?900
cttaccaagg?cttgacatat?accggaaaca?tctagagata?ggtgccccct?tgtggtcggt?960
atacaggtgg?tgcatggctg?tcgtcagctc?gtgtcgtgag?atgttgggtt?aagtcccgca?1020
acgagcgcaa?cccttgttct?gtgttgccag?catgcccttc?ggggtgatgg?ggactcacag?1080
gagactgccg?gggtcaactc?ggaggaaggt?ggggacgacg?tcaagtcatc?atgcccctta?1140
tgtcttgggc?tgcacacgtg?ctacaatggc?cggtacaaag?agctgcgaag?ccgtgaggcg?1200
gagcgaatct?caaaaagccg?gtctcagttc?ggattggggt?ctgcaactcg?accccatgaa?1260
gtcggagttg?ctagtaatcg?cagatcagca?ttgctgcggt?gaatacgttc?ccgggccttg?1320
tacacaccgc?ccgtcacgtc?acgaaagtcg?gtaacacccg?aagccggtgg?cccaacccct?1380
tgtgggaggg?agct?1394。
The seed culture condition of this streptomyces strain (Streptomyces sp.) ZX01 is:
Under 28 ℃, 120rpm condition, be placed on and cultivate 36h on the substratum; Said substratum consists of: glucose 10g/L, yeast extract paste 10g/L, sodium-chlor 2.5g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.5g/L, three water potassium hydrogenphosphate 0.5g/L, pH value 7.2.
Through evidence, streptomyces strain ZX01 of the present invention can be used for preparing the anti-plant viral disease preparation after fermentation culture.
Fermentation culture conditions is following:
Under 28 ℃, 180rpm condition, cultivate 7d; Substratum consists of: millet 10g/L, glucose 10g/L, lime carbonate 2g/L, sodium-chlor 2.5g/L, peptone 3g/L, pH7.2～7.4.
Through applicant's checking, the fermented liquid after the bacterial strain ZX01 fermentation contains and suppresses the material that TMV, CMV and BYDV duplicate, breed, and can be used for preparing the anti-plant viral disease preparation.
Description of drawings
Fig. 1 is the synoptic diagram that streptomycete ZX01 evolutionary tree is analyzed.
Below in conjunction with accompanying drawing and specific embodiment the present invention is done further explain.
Embodiment
The separation in the soil sample that gather in Xinjiang of China Ka Nasi lake of the application's seminar obtains a bacterial strain, and the applicant is with its called after ZX01, and the seed culture condition of this bacterial strain ZX01 is:
Under 28 ℃, 120rpm condition, cultivate 36h; Substratum consists of: glucose: 10g/L, yeast extract paste: 10g/L, sodium-chlor: 2.5g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.5g/L, three water potassium hydrogenphosphate: 0.5g/L, pH value 7.2.
Identify and the 16SrDNA sequential analysis confirms that tentatively this bacterial strain ZX01 is a streptomyces through morphology; Discover that further the fermented liquid of this bacterial strain has stronger inhibition, passivation and therapeutic action to TMV, CMV and BYDV.
Fermentation culture conditions is following:
Under 28 ℃, 180rpm condition, cultivate 7d; Substratum consists of: millet 10g/L, glucose 10g/L, lime carbonate 2g/L, sodium-chlor 2.5g/L, peptone 3g/L, pH7.2～7.4.Fermented liquid after fermentation can directly be used to prepare the anti-plant viral disease preparation.
Streptomycete ZX01 of the present invention submits Chinese common micro-organisms preservation center preservation on May 24th, 2011, and preserving number is CGMCC No.4893.
1, the evaluation of bacterial strain ZX01
The sequence of the morphological specificity of bacterial strain ZX01 of the present invention, cultural characteristic, physiological and biochemical property and 16SrDNA and bacterium classification result are following:
Bacterial strain ZX01 is well-grown (seeing table 1) on most of substratum, bacterium colony fine hair shape on the Gao Shi substratum, and substrate mycelium does not have tabula, does not rupture, and the aerial hyphae growth is vigorous, spore cylindricality, smooth surface.The ZX01 bacterial strain does not all have soluble pigment and produces on various substratum.
Table 1: the cultural characteristic of bacterial strain ZX01
The physio-biochemical characteristics of bacterial strain ZX01 and utilization of carbon source situation are seen table 2.Bacterial strain ZX01 gelatine liquefication and nitrate reduction reaction are all positive.Glucose capable of using, D-ribose, sucrose, D-wood sugar, D-N.F,USP MANNITOL, D-semi-lactosi, rhamnosyl, pectinose and Mierocrystalline cellulose can not utilize D-fructose and inositol as sole carbon source.
Table 2: bacterial strain ZX01 part physiological and biochemical property
| Characteristic | H-4-2 | Characteristic | H-4-2 |
| Gelatine liquefication | + | Sucrose | + |
| The starch hydrolysis | + | The D-wood sugar | + |
| Cellulose utilization | ++ | D-N.F,USP MANNITOL | + |
| Nitrate reduction | + | Inositol | - |
| D-fructose | - | The D-semi-lactosi | + |
| Glucose | ++ | Rhamnosyl | ++ |
| D-ribose | + | Pectinose | + |
Annotate: "+" is positive reaction; "-" is negative reaction; " ++ " is the strong and weak degree of reaction.
The 16SrDNA sequence of streptomycete ZX01 of the present invention is as follows:
tgcagtcgaa?cgatgaaccc?acttcggtgg?gggattagtg?gcgaacgggt?gagtaacacg?60
tgggcaatct?gcccttcact?ctgggacaag?ccctggaaac?ggggtctaat?accggatacg?120
accacttcag?gcatctgatg?gtggtggaaa?gctccggcgg?tgaaggatga?gcccgcggcc?180
tatcagcttg?ttggtggggt?aatggcccac?caaggcgacg?acgggtagcc?ggcctgagag?240
ggcgaccggc?cacactggga?ctgagacacg?gcccagactc?ctacgggagg?cagcagtggg?300
gaatattgca?caatgggcga?aagcctgatg?cagcgacgcc?gcgtgaggga?tgacggcctt?360
cgggttgtaa?acctctttca?gcagggaaga?agcgaaagtg?acggtacctg?cagaagaagc?420
gccggctaac?tacgtgccag?cagccgcggt?aatacgtagg?gcgcaagcgt?tgtccggaat?480
tattgggcgt?aaagagctcg?taggcggctt?gtcacgtcgg?atgtgaaagc?ccggggctta?540
accccgggtc?tgcattcgat?acgggcaggc?tagagtgtgg?taggggagat?cggaattcct?600
ggtgtagcgg?tgaaatgcgc?agatatcagg?aggaacaccg?gtggcgaagg?cggatctctg?660
ggccattact?gacgctgagg?agcgaaagcg?tggggagcga?acaggattag?ataccctggt?720
agtccacgcc?gtaaacgttg?ggaactaggt?gttggcgaca?ttccacgtcg?tcggtgccgc?780
agctaacgca?ttaagttccc?cgcctgggga?gtacggccgc?aaggctaaaa?ctcaaaggaa?840
ttgacggggg?cccgcacaag?cagcggagca?tgtggcttaa?ttcgacgcaa?cgcgaagaac?900
cttaccaagg?cttgacatat?accggaaaca?tctagagata?ggtgccccct?tgtggtcggt?960
atacaggtgg?tgcatggctg?tcgtcagctc?gtgtcgtgag?atgttgggtt?aagtcccgca?1020
acgagcgcaa?cccttgttct?gtgttgccag?catgcccttc?ggggtgatgg?ggactcacag?1080
gagactgccg?gggtcaactc?ggaggaaggt?ggggacgacg?tcaagtcatc?atgcccctta?1140
tgtcttgggc?tgcacacgtg?ctacaatggc?cggtacaaag?agctgcgaag?ccgtgaggcg?1200
gagcgaatct?caaaaagccg?gtctcagttc?ggattggggt?ctgcaactcg?accccatgaa?1260
gtcggagttg?ctagtaatcg?cagatcagca?ttgctgcggt?gaatacgttc?ccgggccttg?1320
tacacaccgc?ccgtcacgtc?acgaaagtcg?gtaacacccg?aagccggtgg?cccaacccct?1380
tgtgggaggg?agct?1394。
The 16SrDNA sequencing entrusts spun gold auspicious bio tech ltd in Nanjing to carry out; Specific as follows: as to extract genomic dna from new fresh thalli with the bacteriolyze enzyme process; Adopt universal primer to carry out the 16SrDNA amplification, the PCR product is sent to the auspicious bio tech ltd of Nanjing spun gold after detecting.Effectively delivering kind of the tree-shaped figure of the phylogeny that the 16SrDNA sequence construct goes out (Fig. 1) from bacterial strain ZX01 and streptomyces can find out: all reference bacterial strains form three big branches altogether.The 16S rDNA sequence similarity of all bacterial strains is between 98.5%～99.1% among bacterial strain ZX01 and the branch III; The closest with the Phylogenetic Relationships of Streptomyces lavendofoliae Kuchaeva; Sequence similarity between them is 99.1%, this with belong at present define in 16SrDNA sequence similarity 95% the standard of being not less than be consistent.But this bacterial strain is not met with any known bacterial classification on systematic evolution tree, but independently becomes to prop up with higher evolution difference, explains that each type strain of this bacterial strain and the same III of branch all exists than big-difference on evolving.
2, bacterial strain ZX01 is active to the inhibition of TMV
(1) bacterial strain ZX01 inhibition effect that TMV is just infected
It is centrifugal to cultivate the tunning of 7d at 28 ℃, 180rpm condition bottom fermentation, and supernatant is subsequent use.Choosing healthy, eugonic 5～6 leaf phase Nicotiana glutinosas is withered spot host, and the whole strain of fermented liquid is smeared, and clear water is smeared in the whole strain of contrast, respectively at whole strain inoculation TMV virus (10 μ g/mL) behind dispenser 12h, 24h and the 48h; Measure the prophylactic effect of bacterial strain ZX01 fermented liquid to TMV virus infection Nicotiana glutinosa.The result sees table 3.
Table 3: the inhibition effect that bacterial strain ZX01 fermented liquid just infects TMV
Annotate: the virus of A working concentration is 500mg/L; Fermented liquid is a fermenation raw liquid.
(2) bacterial strain ZX01 is to the inhibition effect of TMV proliferation function
Adopt whole strain method to measure fermented liquid the TMV virus particle is duplicated the inhibition of proliferation effect in the lobus cardiacus smoke.Choosing healthy, eugonic 5～6 leaf phase Nicotiana glutinosas is withered spot host, and with TMV virus frictional inoculation whole blade, with bacterial strain fermentation liquor coated Nicotiana glutinosa, contrast is put in order strain and smeared clear water behind 6h, 12h and the 24h.The result sees table 4.
Table 4: bacterial strain ZX01 fermented liquid duplicates the inhibition of proliferation effect to TMV in the lobus cardiacus smoke
Annotate: the virus of A working concentration is 500mg/L; Fermented liquid is a fermenation raw liquid.
Adopt the leaf disk method to measure fermented liquid duplicates propagation in common tobacco K326 body to TMV effect.Choose well-grown common cigarette K326; Behind the blade inoculation TMV 6h; Use punch tool on the inoculation tobacco leaf, to break into the leaf disc of diameter as 12mm, leaf disc is flown in fermented liquid and the virus of A soup (mass concentration is 0.5mg/mL), virus inoculation is towards last; And respectively with the leaf disc that flies at virus inoculation in the zero(ppm) water and the not positive contrast of health tobacco leaf disc and the negative control of virus inoculation, 10 leaf discs of every processing.After handling 48h the PBST damping fluid of each leaf disc with 1: 10 (w/v) encapsulated, centrifugal after grinding, supernatant is with the OD value at its 405nm place of indirect elisa method mensuration, and the result sees table 5.
Table 5: bacterial strain ZX01 fermented liquid to TMV in common cigarette inhibition of proliferation effect
| Handle | Average OD 405Value | Viral level/μ g/mL | Inhibiting rate/% |
| Fermented liquid | ?0.6227 | 1.4835 | 53.27 |
| Virus of A | ?0.6218 | 1.4809 | 53.35 |
| CK(+) | ?1.2162 | 3.1744 | ?- |
| CK(-) | ?0.1094 | - | ?- |
Annotate: the virus of A working concentration is 500mg/L; Fermented liquid is a fermenation raw liquid.
3, bacterial strain ZX01 is active to the inhibition of CMV
To supply to be seeded in the seedling pan after the examination tobacco K326 seed disinfection, and place the solarium to cultivate, virus inoculation when treating that the cigarette seedling has 5～7 true leaves.Adopt artificial frictional inoculation, investigate the incidence of cigarette strain behind the inoculation 20d respectively.Can find out that by result's (table 6) preventive effect of processing and result of treatment are respectively 63.91% and 52.48%.
Table 6: bacterial strain ZX01 fermented liquid is to the control effect of yellow stunt of wheat
Annotate: the virus of A working concentration is 500mg/L; Fermented liquid is a fermenation raw liquid.
4, bacterial strain ZX01 is active to the inhibition of BYDV
In the wheat phase of standing up barly yellow dwarf virus GAV strain system is inoculated on the wheat, in flowering stage of wheat statistics sickness rate and disease index.Can find out that by result's (table 6) preventive effect of processing and result of treatment are respectively 58.36% and 46.82%.
Table 6: bacterial strain ZX01 fermented liquid is to the control effect of yellow stunt of wheat
Annotate: the virus of A working concentration is 500mg/L; Fermented liquid is a fermenation raw liquid.
Result according to above instance shows; Bacterial strain ZX01 of the present invention is through liquid fermentation and culture; Contain in its fermented liquid and suppress the material that TMV, CMV and BYDV duplicate, breed; Can significantly alleviate the generation of virus disease, be expected to be developed as the microbial reagent of a kind of novel control plant virus.
tgcagtcgaa?cgatgaaccc?acttcggtgg?gggattagtg?gcgaacgggt?gagtaacacg?60
tgggcaatct?gcccttcact?ctgggacaag?ccctggaaac?ggggtctaat?accggatacg?120
accacttcag?gcatctgatg?gtggtggaaa?gctccggcgg?tgaaggatga?gcccgcggcc?180
tatcagcttg?ttggtggggt?aatggcccac?caaggcgacg?acgggtagcc?ggcctgagag?240
ggcgaccggc?cacactggga?ctgagacacg?gcccagactc?ctacgggagg?cagcagtggg?300
gaatattgca?caatgggcga?aagcctgatg?cagcgacgcc?gcgtgaggga?tgacggcctt?360
cgggttgtaa?acctctttca?gcagggaaga?agcgaaagtg?acggtacctg?cagaagaagc?420
gccggctaac?tacgtgccag?cagccgcggt?aatacgtagg?gcgcaagcgt?tgtccggaat?480
tattgggcgt?aaagagctcg?taggcggctt?gtcacgtcgg?atgtgaaagc?ccggggctta?540
accccgggtc?tgcattcgat?acgggcaggc?tagagtgtgg?taggggagat?cggaattcct?600
ggtgtagcgg?tgaaatgcgc?agatatcagg?aggaacaccg?gtggcgaagg?cggatctctg?660
ggccattact?gacgctgagg?agcgaaagcg?tggggagcga?acaggattag?ataccctggt?720
agtccacgcc?gtaaacgttg?ggaactaggt?gttggcgaca?ttccacgtcg?tcggtgccgc?780
agctaacgca?ttaagttccc?cgcctgggga?gtacggccgc?aaggctaaaa?ctcaaaggaa?840
ttgacggggg?cccgcacaag?cagcggagca?tgtggcttaa?ttcgacgcaa?cgcgaagaac?900
cttaccaagg?cttgacatat?accggaaaca?tctagagata?ggtgccccct?tgtggtcggt?960
atacaggtgg?tgcatggctg?tcgtcagctc?gtgtcgtgag?atgttgggtt?aagtcccgca?1020
acgagcgcaa?cccttgttct?gtgttgccag?catgcccttc?ggggtgatgg?ggactcacag?1080
gagactgccg?gggtcaactc?ggaggaaggt?ggggacgacg?tcaagtcatc?atgcccctta?1140
tgtcttgggc?tgcacacgtg?ctacaatggc?cggtacaaag?agctgcgaag?ccgtgaggcg?1200
gagcgaatct?caaaaagccg?gtctcagttc?ggattggggt?ctgcaactcg?accccatgaa?1260
gtcggagttg?ctagtaatcg?cagatcagca?ttgctgcggt?gaatacgttc?ccgggccttg?1320
tacacaccgc?ccgtcacgtc?acgaaagtcg?gtaacacccg?aagccggtgg?cccaacccct?1380
tgtgggaggg?agct?1394
Claims (5)
1. a streptomyces strain (Streptomyces sp.) ZX01 is characterized in that its deposit number is CGMCC No.4893.
2. streptomyces strain as claimed in claim 1 (Streptomyces sp.) ZX01 is characterized in that the 16SrDNA sequence of this streptomyces strain is as follows:
tgcagtcgaa?cgatgaaccc?acttcggtgg?gggattagtg?gcgaacgggt?gagtaacacg?60?tgggcaatct
gcccttcact?ctgggacaag?ccctggaaac?ggggtctaat?accggatacg?120
accacttcag?gcatctgatg?gtggtggaaa?gctccggcgg?tgaaggatga?gcccgcggcc?180
tatcagcttg?ttggtggggt?aatggcccac?caaggcgacg?acgggtagcc?ggcctgagag?240
ggcgaccggc?cacactggga?ctgagacacg?gcccagactc?ctacgggagg?cagcagtggg?300
gaatattgca?caatgggcga?aagcctgatg?cagcgacgcc?gcgtgaggga?tgacggcctt?360
cgggttgtaa?acctctttca?gcagggaaga?agcgaaagtg?acggtacctg?cagaagaagc?420
gccggctaac?tacgtgccag?cagccgcggt?aatacgtagg?gcgcaagcgt?tgtccggaat?480
tattgggcgt?aaagagctcg?taggcggctt?gtcacgtcgg?atgtgaaagc?ccggggctta?540
accccgggtc?tgcattcgat?acgggcaggc?tagagtgtgg?taggggagat?cggaattcct?600
ggtgtagcgg?tgaaatgcgc?agatatcagg?aggaacaccg?gtggcgaagg?cggatctctg?660
ggccattact?gacgctgagg?agcgaaagcg?tggggagcga?acaggattag?ataccctggt?720
agtccacgcc?gtaaacgttg?ggaactaggt?gttggcgaca?ttccacgtcg?tcggtgccgc?780
agctaacgca?ttaagttccc?cgcctgggga?gtacggccgc?aaggctaaaa?ctcaaaggaa?840
ttgacggggg?cccgcacaag?cagcggagca?tgtggcttaa?ttcgacgcaa?cgcgaagaac?900
cttaccaagg?cttgacatat?accggaaaca?tctagagata?ggtgccccct?tgtggtcggt?960
atacaggtgg?tgcatggctg?tcgtcagctc?gtgtcgtgag?atgttgggtt?aagtcccgca?1020
acgagcgcaa?cccttgttct?gtgttgccag?catgcccttc?ggggtgatgg?ggactcacag?1080
gagactgccg?gggtcaactc?ggaggaaggt?ggggacgacg?tcaagtcatc?atgcccctta?1140
tgtcttgggc?tgcacacgtg?ctacaatggc?cggtacaaag?agctgcgaag?ccgtgaggcg?1200
gagcgaatct?caaaaagccg?gtctcagttc?ggattggggt?ctgcaactcg?accccatgaa?1260
gtcggagttg?ctagtaatcg?cagatcagca?ttgctgcggt?gaatacgttc?ccgggccttg?1320
tacacaccgc?ccgtcacgtc?acgaaagtcg?gtaacacccg?aagccggtgg?cccaacccct?1380
tgtgggaggg?agct?1394。
3. the described streptomyces strain of claim 1 (Streptomyces sp.) ZX01 is characterized in that, the seed culture condition of this streptomyces strain (Streptomyces sp.) ZX01 is:
Under 28 ℃, 120rpm condition, be placed on culture medium culturing 36h; Said substratum consists of: glucose: 10g/L, yeast extract paste: 10g/L, sodium-chlor: 2.5g/L, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.5g/L, three water potassium hydrogenphosphate: 0.5g/L, pH value 7.2.
4. claim 1 or 2 described streptomyces strains (Streptomyces sp.) ZX01 are used to prepare the application of anti-plant viral disease preparation.
5. application as claimed in claim 4 is characterized in that, the fermented liquid of described streptomyces strain (Streptomyces sp.) ZX01 after fermentation culture directly is used to prepare the anti-plant viral disease preparation, and described fermentation culture conditions is:
Under 28 ℃, 180rpm condition, cultivate 7d; Substratum consists of: millet: 10g/L, glucose: 10g/L, lime carbonate: 2g/L, sodium-chlor: 2.5g/L, peptone: 3g/L, pH value 7.2～7.4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110301736 CN102337239B (en) | 2011-10-08 | 2011-10-08 | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110301736 CN102337239B (en) | 2011-10-08 | 2011-10-08 | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102337239A true CN102337239A (en) | 2012-02-01 |
| CN102337239B CN102337239B (en) | 2013-02-06 |
Family
ID=45513193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110301736 Active CN102337239B (en) | 2011-10-08 | 2011-10-08 | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102337239B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130965A (en) * | 2014-08-06 | 2014-11-05 | 东北林业大学 | Streptomyces with inhibiting effect on poplar gray leaf spot pathogen |
| CN104130965B (en) * | 2014-08-06 | 2017-01-04 | 东北林业大学 | One strain has the streptomycete of inhibitory action to willow Cercospora Sojina Hara |
| CN108812686A (en) * | 2018-04-28 | 2018-11-16 | 杨凌农科大无公害农药研究服务中心 | A kind of microbial source antiviral composition, preparation and its application of the GP-1 containing glycoprotein |
| CN111411050A (en) * | 2019-01-04 | 2020-07-14 | 西北农林科技大学 | F01 actinomycete and antiviral application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181390A (en) * | 2011-03-21 | 2011-09-14 | 北京市农林科学院 | Streptomyces parvus strain and application thereof |
-
2011
- 2011-10-08 CN CN 201110301736 patent/CN102337239B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181390A (en) * | 2011-03-21 | 2011-09-14 | 北京市农林科学院 | Streptomyces parvus strain and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| ABDEL-MONEIM M.GALAL: "Induction of Systemic Acquired Resistance in Cucumber Plant Against Cucumber Mosaic Cucumovirus by Local Streptomyces Strains", 《PLANT PATHOLOGY JOURNAL》, vol. 5, no. 3, 31 December 2006 (2006-12-31), pages 343 - 349 * |
| ZHANG,X.等: "Accession Number JN572690.1", 《GENBANK》, 14 September 2011 (2011-09-14), pages 1 - 2 * |
| 于银霞: "168号放线菌发酵产物的分离提取和活性研究", 《中国优秀硕士学位论文全文数据库基础科学辑》, no. 10, 15 October 2009 (2009-10-15), pages 006 - 168 * |
| 徐路明 等: "土壤放线菌X1的筛选及其抑菌活性初探", 《中国农学通报》, vol. 27, no. 9, 31 March 2011 (2011-03-31), pages 195 - 200 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130965A (en) * | 2014-08-06 | 2014-11-05 | 东北林业大学 | Streptomyces with inhibiting effect on poplar gray leaf spot pathogen |
| CN104130965B (en) * | 2014-08-06 | 2017-01-04 | 东北林业大学 | One strain has the streptomycete of inhibitory action to willow Cercospora Sojina Hara |
| CN108812686A (en) * | 2018-04-28 | 2018-11-16 | 杨凌农科大无公害农药研究服务中心 | A kind of microbial source antiviral composition, preparation and its application of the GP-1 containing glycoprotein |
| CN111411050A (en) * | 2019-01-04 | 2020-07-14 | 西北农林科技大学 | F01 actinomycete and antiviral application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102337239B (en) | 2013-02-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2736382C1 (en) | Compositions and methods for fusarium disease control | |
| CN105733982B (en) | Bacillus amyloliquefaciens, microbial inoculum and preparation method thereof for preventing and treating two born of the same parents' branch-rot of blueberry hair color | |
| CN104673705B (en) | One plant prevent and treat wheat scab bacillus amyloliquefaciens and its application | |
| CN102181390B (en) | Streptomyces parvus strain and application thereof | |
| CN107034146A (en) | A kind of Antagonistic Trichoderma for promoting crop growth and its application | |
| CN104762230B (en) | Prevent and treat Shewanella alga and its application of crop storage phase Aspergillus flavus and toxin | |
| CN104130958A (en) | Bacillus subtilis and application of bacillus subtilis in preventing and curing peony root-knot nematode | |
| CN112021067A (en) | Method for breeding new tremella aurantialba variety through combination of asexual spores of tremella aurantialba and stereum hirsutum | |
| CN102851225B (en) | Stenotrophomonas acidaminiphila and application in control of apple tree canker thereof | |
| CN105441331A (en) | Myrothecium roridum and application thereof | |
| CN104673723B (en) | A kind of extremely long streptomyces strain SL01 and its microbial bacterial agent and application | |
| CN111280183B (en) | Apple anthracnose leaf blight biocontrol microbial inoculum and preparation method and application thereof | |
| CN101375689B (en) | Black oyster mushroom extract as well as preparation method and application thereof | |
| CN102337239B (en) | Streptomyces sp. strain and application thereof for preparing formulation for inhibiting plant virus diseases | |
| CN109609403B (en) | Biocontrol bacterium and application thereof in prevention and control of downy mildew of crops | |
| CN113817642B (en) | Bacillus bailii YJ02, microbial preparation and application thereof | |
| CN102787085B (en) | Strain of endophytic Streptomyces lydicus DOR3-2 of Chinese yam and its application | |
| CN109266559A (en) | A kind of application of Trichoderma harzianum LTR-2 | |
| CN107058124A (en) | A kind of Nomuraea rileyi strain and the application on light sword spodoptera is prevented and treated | |
| Matsumoto et al. | Effect of cropping history on the population structure of Typhula incarnata and Typhula ishikariensis | |
| CN113234601A (en) | Marine fungus new species and application thereof in drought stress resistance of plants | |
| CN106244510B (en) | One bacillus firmus and its application in terms of prevention and treatment fig, pomegranate anthracnose | |
| CN111187732A (en) | Biocontrol strain for preventing and treating bitter gourd fusarium wilt and application thereof | |
| CN111394262B (en) | Weed biocontrol bacterium and application thereof | |
| CN115044494B (en) | Bacillus subtilis WZ10 and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: YANGLING FUJI BIO-TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: YANGLING NONGKEDA RESEARCH + DEVELOPMENT CENTER OF BIORATIONAL PESTICIDE Effective date: 20141230 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 712100 XI AN, SHAANXI PROVINCE TO: 712100 XIANYANG, SHAANXI PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20141230 Address after: 712100, Shaanxi Yangling Demonstration Zone Industrial Park Yang Yang Road West of the south side Patentee after: Yangling Fu Ji Biological Technology Co. Ltd. Address before: 712100 Shaanxi Province, Xi'an city Yangling District Tai Road No. 3 demonstration Patentee before: Yangling Nongkeda Research & Development Center of Biorational Pesticide |