CN115044494B - Bacillus subtilis WZ10 and application thereof - Google Patents
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention provides bacillus subtilis WZ10 and application thereof, and belongs to the technical field of biological control. The bacillus subtilis WZ10 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 24008. The bacillus subtilis WZ10 provided by the invention can not only prevent and treat potato wilt, but also promote potato growth and increase potato yield.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to bacillus subtilis WZ10 and application thereof.
Background
Potatoes are a world staple food crop and are also important economic crops in China, and the potato is widely planted in China and mainly distributed in areas such as inner Mongolia, gansu, yunnan, hebei and Heilongjiang. Inner Mongolia is used as a main planting area of potatoes, the planting area and the yield are continuously increased, and then soil-borne diseases are aggravated year by year, and especially potato wilt is an important disease threatening the industry in recent years. Potato blight is an important soil-borne disease caused by Fusarium spp, and is widely distributed worldwide. Pathogenic bacteria starts to infect to the vascular bundles from the root, so that the vascular bundles become brown and blocked, plants wilt to die, irregular brown vigor rings appear at the vascular bundles in the split potato blocks, the incidence rate of infected plots can reach more than 70% and even the disease is stopped, and the yield and quality of potatoes are seriously influenced.
The phenomena of high residue and abuse of chemical pesticides are common in the potato industry, and cause ecological unbalance and environmental pollution of soil. The use of pesticides is reduced by using measures such as biological control and the like, and the method has the advantages of environmental protection, ecological safety and the like. At present, plant endophytic bacteria, especially bacillus, are utilized for biological control, and the application prospect is quite broad. Endophytic bacteria form a reciprocal co-existence relationship with host plants during long-term co-evolution. Bacillus can directly induce plants to produce disease resistance, and also can induce plants to produce protein polypeptide antibacterial substances, induce host plant defense related protein accumulation, synthesis of phytoalexin and other secondary metabolites, production of hydrolase and oxidase, and expression of plant defense related enzymes; a molecular signaling pathway that induces plants to alter disease resistance, or to induce expression of defensive genes in host plants, and the like. Meanwhile, the endophytic bacillus can improve the disease resistance of a host through the growth promotion effect on plants. It has been found that endophytic bacillus can induce plant to produce hormone or secondary metabolite to promote plant growth, such as promoting seed germination, increasing seedling survival rate and biomass of roots, stems, leaves, etc., or promote plant growth by changing activity of plant growth-development related enzymes to increase disease resistance. Some bacillus in plants has obvious inhibiting effect on fusarium oxysporum, and has obvious growth promoting and yield increasing effects while inducing plant disease resistance. However, few microorganisms are currently applied to prevention and control of potato blight, and particularly antagonistic strains capable of achieving both growth promotion and high efficiency and application methods thereof are lacking.
Disclosure of Invention
In view of the above, the invention aims to provide the bacillus subtilis WZ10 and the application thereof, and the bacillus subtilis WZ10 provided by the invention can not only prevent and treat potato wilt, but also promote potato growth and improve potato yield.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a bacillus subtilis (Bacillus subtilis) WZ10, wherein the bacillus subtilis WZ10 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 24008.
The invention also provides application of the bacillus subtilis WZ10 in preventing and treating potato wilt.
Preferably, the potato blight is caused by fusarium oxysporum.
Preferably, the application comprises: and (3) seed dressing is carried out on the bacterial liquid containing the bacillus subtilis WZ10 and the potatoes, and then the potatoes are planted.
Preferably, the amount of bacterial liquid required per kilogram of potato is 20mL, and the effective viable count of bacillus subtilis WZ10 in the bacterial liquid is 1 multiplied by 10 8 cfu/mL。
The invention also provides application of the bacillus subtilis WZ10 in promoting potato growth.
Preferably, the growth comprises plant height, root length or fresh weight.
Preferably, the application comprises: bacterial liquid containing bacillus subtilis WZ10 is inoculated to the root of potato plants.
Preferably, the amount of inoculated bacterial liquid of each potato plant is 10mL, and the effective viable count of bacillus subtilis WZ10 in the bacterial liquid is 1 multiplied by 10 6 cfu/mL。
The invention also provides application of the bacillus subtilis WZ10 in improving potato yield.
The invention provides a bacillus subtilis (Bacillus subtilis) WZ10, wherein the bacillus subtilis WZ10 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 24008. The bacillus subtilis WZ10 provided by the invention can not only prevent and treat potato wilt, but also promote potato growth and increase potato yield.
Preservation description
The bacillus subtilis WZ10 is Bacillus subtilis, is preserved in China general microbiological culture Collection center (CGMCC) at the date of 11 and 30 of 2021, and has the preservation number of CGMCC No.24008, which is the institute of microorganisms, national academy of sciences of China, no. 1, no. 3, north Chen West Lu, korea, beijing, city.
Drawings
FIG. 1 is a colony characterization of Bacillus subtilis WZ 10;
FIG. 2 shows the plate-stand result of Bacillus subtilis WZ10 against Fusarium oxysporum;
FIG. 3 shows the indoor control effect of Bacillus subtilis WZ10 on potato wilt, with the left side treated with Bacillus subtilis WZ10 and the right side treated with control;
FIG. 4 shows the effect of B.subtilis WZ10 on potato growth, left B.subtilis WZ10 treatment, right control.
Detailed Description
The invention provides a bacillus subtilis (Bacillus subtilis) WZ10, wherein the bacillus subtilis WZ10 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 24008.
The invention also provides application of the bacillus subtilis WZ10 in preventing and treating potato wilt.
In the present invention, the potato blight is preferably caused by fusarium oxysporum. In the present invention, the application includes: and (3) mixing the bacterial liquid containing the bacillus subtilis WZ10 with potato seeds, and then planting the potatoes. In the present invention, the amount of bacterial liquid required per kilogram of potato is preferably 20mL. In the present invention, the effective viable count of Bacillus subtilis WZ10 in the bacterial liquid is preferably 1X 10 8 cfu/mL. The preparation method of the bacterial liquid containing the bacillus subtilis WZ10 is not particularly limited, and a person skilled in the art can adopt a conventional method for culturing the bacillus subtilis, and in the specific embodiment of the invention, the method specifically comprises the following steps: inoculating the activated bacillus subtilis into 250mL triangular flask (100 mL per bottle) liquid culture medium, and shaking at 27deg.C with shaking table rotation speed of 150r.min -1 Fermenting for 3d to obtain bacterial liquid. In the present invention, the liquid medium preferably has the formula: in weight percent, tryptone 1.5%, yeast extract 0.58%, naCl0.54%, and the balance of distilled water, pH 6.8. The invention is to activate bacteriaThe culture medium and the activation method used in the seed are not particularly limited, and in the specific embodiment of the present invention, the culture medium is Luria-Bertani culture medium (LB): 10g of tryptone, 5g of yeast extract, 5g of NaCl, 20g of agar and 1000mL of distilled water. The activation method is that the strain is inoculated on LB plate and cultured for 3d at 28 ℃.
The invention also provides application of the bacillus subtilis WZ10 in promoting potato growth.
In the present invention, the growth preferably includes plant height, root length or fresh weight. In the present invention, the fresh weight refers to the weight of the whole plant. In the present invention, the application preferably includes: bacterial liquid containing bacillus subtilis WZ10 is inoculated to the root of potato plants. In the invention, the amount of the inoculated bacterial liquid of each potato plant is preferably 10mL, and the effective viable count of the bacillus subtilis WZ10 in the bacterial liquid is preferably 1X 10 6 cfu/mL. The preparation method of the bacterial liquid is not particularly limited, and the bacterial liquid is prepared by the preparation method and is not repeated here.
The invention also provides application of the bacillus subtilis WZ10 in improving potato yield.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparation of a fermentation broth of bacillus subtilis WZ 10: the strain is prepared by liquid fermentation after plate activation, and the specific method is as follows:
1. plate activation
The culture medium formula is Luria-Bertani culture medium (LB): 10g of tryptone, 5g of yeast extract, 5g of NaCl, 20g of agar and 1000mL of distilled water. The strain was inoculated on LB plate and cultured at 28℃for 3d.
2. Liquid fermentation culture
The formula is as follows: in weight percent, tryptone 1.5%, yeast extract 0.58%, naCl0.54%, and the balance distilled water, pH 6.8. Inoculating the activated strain into 250mL triangular flask (100 mL per bottle) liquid culture mediumAt the temperature of 27 ℃, the rotating speed of the shaking table is 150 r.min -1 Fermenting for 3d to obtain fermentation liquor of bacillus subtilis WZ10, wherein the effective viable count of bacillus subtilis WZ10 is 1 multiplied by 10 8 cfu/mL。
Example 2
Identification of bacillus subtilis WZ 10:
1. culture of strains
Strain WZ10 was cultured for use as in the plate activation and liquid fermentation method of example 1.
2. Identification of Strain WZ10
Bacterial strain WZ10 is cultured on LB solid medium for 24 hours at 25 ℃, the bacterial colony is round, milky white, rough in surface after being cultured for a plurality of days, sticky and difficult to pick up, and obvious corrugation and bulge are formed around the bacterial colony. Bacterial genome DNA is extracted by adopting a CTAB method, 16S universal primers 27F and 1492R, gyrB gene amplification primers UP1 and UP2R (refer to documents Yu Guohui, niu Chunyan, chen Yuanfeng, and the like), and 16S rDNA and gyrB gene fragments are amplified by adopting a conventional method by utilizing 16S rDNA to combine gyrA and gyrB genes for rapid identification of biocontrol bacillus R31 [ J ]. Chinese biological control, 2010 (2): 7). After the PCR product is qualified through 1% agarose gel electrophoresis, the PCR product is sent to Shanghai Biotechnology Inc. for sequencing, and the obtained sequence is subjected to NCBI online BLAST analysis and is identified as bacillus subtilis (Bacillus subtilis) by combining morphological characteristics.
Example 3
Plate counter assay for bacillus subtilis WZ 10:
1. test strain
Bacillus subtilis WZ10 was cultured for use by the plate activation step of example 1;
fusarium oxysporum FO, a pathogen of potato fusarium wilt, is cultured by a PDA flat plate for standby, and the specific operation is as follows: the pathogenic bacteria were inoculated onto potato dextrose medium (PDA) (potato 200g, glucose 20g, agar 18g, distilled water 1000 mL) plates and incubated at 25℃for 7d.
2. Test method
The bacillus subtilis WZ10 strain is inoculated into a 2 d-cultured fusarium oxysporum FO plate by adopting a plate facing method. In an ultra-clean workbench, a puncher is used for punching a bacillus subtilis WZ10 bacterial cake with the diameter of 5mm, and the bacillus subtilis WZ10 bacterial cake is placed at a position which is 2.5cm away from fusarium oxysporum in the same level for opposite culture; fusarium oxysporum FO plates were incubated for 2d and repeated 3 times as controls. Growth was observed daily, and after exposure of bacillus subtilis WZ10 to fusarium oxysporum hypha FO, the radius of fusarium oxysporum colony was determined and the inhibition was investigated at 5d after inoculation.
The inhibition rate calculation formula: inhibition (%) = [ (control colony radius-treated colony radius)/control colony radius ] ×100%
3. Test results
The result shows that the bacillus subtilis WZ10 has remarkable inhibition effect on fusarium oxysporum FO, the inhibition rate on the fusarium oxysporum FO is 76.67%, and after the bacillus subtilis is cultivated for 3 days in a counter manner, the pathogenic bacteria hypha is not expanded any more, and the bacillus subtilis continues to grow.
TABLE 1 inhibition of Fusarium oxysporum FO by Bacillus subtilis WZ10
| Treatment of | Colony radius (cm) | Inhibition ratio (%) | Significance of difference (P<0.05) |
| WZ10 | 1.05 | 76.67％ | b |
| CK | 4.50 | -- | a |
Example 4
Indoor growth promotion and control test for bacillus subtilis WZ10
1. Preparation of test formulations
Preparing a fermentation broth of bacillus subtilis WZ10 by the method of example 1, and diluting the fermentation broth by 100 times with sterile water for later use;
preparation of fusarium oxysporum FO spore suspension: inoculating fusarium wilt bacteria into wheat bran culture medium (wheat bran 500g, distilled water 200 mL), culturing at 25deg.C for 7d, washing cultured wheat bran with sterile water, filtering with 4 layers of sterilized absorbent gauze to obtain spore suspension, and regulating spore concentration to 10 7 Individual spores/mL.
2. Seed for test
Potato virus-free tissue culture seedling: the variety is Fei Wu Rui Tar.
3. Disease classification standard and disease index calculation for wilt
The index grading criteria for wilt disease are shown in Table 2;
disease index = [ Σ (number of individual stage disease plants×relative stage representative value)/(total number of investigation×5) ]×100.
TABLE 2 in-house classification criteria for potato wilt
4. Test method
Transplanting the potato virus-free tissue culture seedlings into a flowerpot with sterilized nutrient soil, wherein the sterilized soil and the sterilized vermiculite are uniformly mixed according to the volume ratio of 1:1, and each treatment is carried out on 12 pots. When the plant seedling grows to 3-5 leaf stage, the prepared diluted fermentation liquid (with the bacterial content of 1 multiplied by 10) of the bacillus subtilis WZ10 is inoculated by root injury 6 cfu/mL) is inoculated to the root of the plant, and each plant is 10mL; the Control (CK) was prepared without fermentation broth. After 6d, the same procedure was followed for the inoculation of Fusarium oxysporum FO spore suspensions, 10mL each. Maintaining the temperature at 25 ℃ and the relative humidity at 70%, supplementing light by using an LED light source in a climate chamber, culturing in an alternating manner for 12 hours under illumination and darkness, and pouring water once at intervals of 2 days. After 21d, the growth condition of plants is investigated, and the plant height, the stem thickness and the fresh weight are recorded; and (3) after 28d, performing disease investigation, recording disease grade, and calculating disease index and prevention and treatment effect.
5. Test results
The result shows that the bacillus subtilis WZ10 treated plant can obviously improve the growth vigor of potatoes, the plant height is increased by 14.34 percent after treatment, the root length is increased by 19.75 percent, the fresh weight is increased by 77.06 percent, and the plant has obvious wilt prevention and control effect and can reach 80.65 percent.
TABLE 3 Protoffee of Bacillus subtilis WZ10 against Potato
| Height of plant (mm) | Root length (mm) | Fresh weight (g) | |
| WZ10 treatment | 150.56±2.97a | 254.57±4.08a | 26.79±3.34a |
| CK | 131.68±11.42b | 212.58±30.58b | 15.13±1.57b |
TABLE 4 indoor control Effect of Bacillus subtilis WZ10 on potato blight
| Index of disease condition | Control effect (%) | Significance of difference (P<0.05) | |
| WZ10 treatment | 10 | 80.65 | b |
| CK | 51.67 | — | a |
Example 5
Field control test of bacillus subtilis WZ10
1. Preparation of formulations for testing
A fermentation broth of strain WZ10 was prepared as in example 1 for use.
2. Seed potato selection
Seed potato: stock of feilure.
3. Test method
The test is carried out at a dry farming test station of the bald and the helminth county in the inner Mongolia and the Wuchuan county in the huge city, and the potatoes are planted in the continuous cropping field in land areas in successive years. The bacillus subtilis WZ10 fermentation broth (the bacterial content is 1 multiplied by 10) is arranged by adopting a random block design 8 cfu/mL) seed dressing treatment, seed dressing amount of 20mL/kg seed potato, no treatment blank control CK, 3 times of repetition of each treatment, total 6 cells and cell area of 86.4m 2 And (3) performing conventional field management, wherein the row spacing is 90cm, the plant spacing is 25 cm. The disease potato rate and yield were investigated and recorded during the harvest period of potatoes. The disease index grading standard of the disease potato is referred to in Table 5.
TABLE 5 field grading Standard for potato blight
| Disease level | Grading standard |
| 0 | No symptom of the tangential vascular bundle |
| 1 | Less than 25% of the vascular bundles yellow and brown |
| 2 | 26% -50% of vascular bundles yellow and brown |
| 3 | 51% -75% of vascular bundles yellow and brown |
| 4 | Yellowing and browning of more than 76% of vascular bundles |
4. Test results
The result shows that the strain WZ10 has remarkable control effect on potato wilt and yield increasing effect. Compared with a blank control, the control effect reaches 70.78%; yield is increased by 17.15%.
TABLE 6 field control Effect of Bacillus subtilis WZ10 on potato blight
| Treatment of | Index of disease condition | Control effect (%) | Yield (kg/667 m) 2 ) | Yield increase effect (%) |
| WZ10 | 7.37 | 70.78 | 3593.85 | 17.15 |
| CK | 25.22 | - | 3067.74 | - |
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. A strain of bacillus subtilis (Bacillus subtilis) WZ10, wherein the bacillus subtilis WZ10 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 24008.
2. Use of bacillus subtilis WZ10 according to claim 1 for controlling potato wilt.
3. Use according to claim 2, characterized in that the potato blight is caused by fusarium oxysporum.
4. The application according to claim 2, characterized in that it comprises: and (3) seed dressing is carried out on the bacterial liquid containing the bacillus subtilis WZ10 and the potatoes, and then the potatoes are planted.
5. The use according to claim 4, wherein the amount of bacterial liquid required per kilogram of potato is 20mL, and the effective viable count of the bacillus subtilis WZ10 in the bacterial liquid is 1X 10 8 cfu/mL。
6. Use of bacillus subtilis WZ10 according to claim 1 for promoting potato growth.
7. The use of claim 6, wherein the growth comprises plant height, root length, or fresh weight.
8. The application according to claim 6, characterized in that it comprises: bacterial liquid containing bacillus subtilis WZ10 is inoculated to the root of potato plants.
9. The use according to claim 8, wherein the amount of inoculated bacterial liquid per potato plant is 10mL, the bacterial liquid containing the cumic budsThe effective viable count of the bacillus WZ10 is 1 multiplied by 10 6 cfu/mL。
10. Use of bacillus subtilis WZ10 according to claim 1 for increasing potato yield.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726384A (en) * | 2015-04-20 | 2015-06-24 | 黑龙江省科学院微生物研究所 | Bacillus subtilis for prohibiting rhizoctonia solani of potatoes |
| CN106995791A (en) * | 2017-06-09 | 2017-08-01 | 福建省农业科学院植物保护研究所 | A kind of bacillus subtilis BS193 for preventing and treating the late blight of potato and its application |
| CN107603906A (en) * | 2017-09-21 | 2018-01-19 | 天津坤禾生物科技股份有限公司 | A kind of preparation method and application for the bacterial strain and its preparation for preventing and treating potato wilt |
| CN108342335A (en) * | 2017-01-23 | 2018-07-31 | 河北农业大学 | Application of Bacillus belgii NZ-4 in promoting plant growth |
| CN108342336A (en) * | 2017-01-23 | 2018-07-31 | 河北农业大学 | Potato wilt antagonistic bacterium NZ-4 and application thereof |
| CN113215010A (en) * | 2020-01-21 | 2021-08-06 | 中国农业科学院蔬菜花卉研究所 | Bacillus belgii ZF128 and application thereof in preventing and treating potato wilt |
-
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- 2022-04-27 CN CN202210456556.6A patent/CN115044494B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726384A (en) * | 2015-04-20 | 2015-06-24 | 黑龙江省科学院微生物研究所 | Bacillus subtilis for prohibiting rhizoctonia solani of potatoes |
| CN108342335A (en) * | 2017-01-23 | 2018-07-31 | 河北农业大学 | Application of Bacillus belgii NZ-4 in promoting plant growth |
| CN108342336A (en) * | 2017-01-23 | 2018-07-31 | 河北农业大学 | Potato wilt antagonistic bacterium NZ-4 and application thereof |
| CN106995791A (en) * | 2017-06-09 | 2017-08-01 | 福建省农业科学院植物保护研究所 | A kind of bacillus subtilis BS193 for preventing and treating the late blight of potato and its application |
| CN107603906A (en) * | 2017-09-21 | 2018-01-19 | 天津坤禾生物科技股份有限公司 | A kind of preparation method and application for the bacterial strain and its preparation for preventing and treating potato wilt |
| CN113215010A (en) * | 2020-01-21 | 2021-08-06 | 中国农业科学院蔬菜花卉研究所 | Bacillus belgii ZF128 and application thereof in preventing and treating potato wilt |
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