CN108783007A - A kind of fishes and shrimps feed addictive and its preparation method and application - Google Patents
A kind of fishes and shrimps feed addictive and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of fishes and shrimps feed addictives, contain：Herba Verbenae, Sagina subulata extract, Flos Eriocauli extract, herba hedyotis diffusae extract, Herba Agrimoniae extract, Geranium extract, Herba Pyrolae extract.The fishes and shrimps feed addictive of the present invention is pure plant component, without industrial chemicals, it reduces because risk caused by industrial chemicals pollution not only improves the prevention of damage by disease of fish, shrimp using the feed addictive of the present invention, improve survival rate, the digestive ferment secretion level that fish, shrimp can also be improved promotes the healthy growth of fish, shrimp to effectively facilitate nutrient digesting and assimilating in fishes and shrimps body and convert, therefore can be substantially improved fish, shrimp aquaculture industry economic benefit, promote the sound development of industrial chain.
Description
Technical field
The present invention relates to a kind of fishes and shrimps feed addictives and its preparation method and application.
Background technology
Sea-farming is one of the pillar industry of China's aquaculture, the adjustment with aquaculture structure and sustainability
It is extremely urgent to walk industrial aquaculture road for the requirement of road for development.It is to realize that aquatic products health is supported to keep water quality that is good and stablizing
The basis grown, the violent of breeding water body environmental factor change the emergency reaction that can induce cultivation object, directly or indirectly cause to support
Grow the disease outburst of object.
Disease control problem becomes aquaculture development Important Problems the most prominent and urgently to be resolved hurrily, including batch production seawater
Aquaculture similarly faces the serious situation that cause of disease is wreaked havoc, fishes and shrimps are lower for the resistance, breeding seawater constantly deteriorates, for asking above
Topic, the way of industry is at present：It improves cultural method and uses medical treatment.But due to blindly even violated with abuse antibiotic
The aquatic product quality that drug etc. causes cultivation to list declines, and brings threat to the edible safety of the aquatic products such as cultivation fish, shrimp, and dirty
Ecological environment is contaminated, the sustainable development of China's sea-farming is directly affected.
Grouper cultivating annual output is up to 100,000 tons or more, nearly 10,000,000,000 yuan of the output value；Prawn culturing annual output bigger, such as South America
For white shrimp annual output in 2015 up to 1,620,000 tons, the output value surpasses 60,000,000,000 yuan, it has also become the mainstay industry of China's sea-farming.But
Due to the presence of disease problem, the serious further development for restricting mariculture industry, providing a kind of can effectively prevent fish, shrimp
The method of disease problem is necessary in breeding process.
Chinese herbal medicine is at low cost with its, pure natural, toxic side effect is small, the original advantage such as multi-functional, is more and more answered
It uses in feed addictive, but the feed addictive presence of existing addition Chinese herbal medicine needs medicinal herb components and other compositions
It is used cooperatively, preparation process is complicated, or the problem that formula is unreasonable, using effect is bad.
Disclose a kind of epinephelus feed additive and preparation method thereof in CN105724766A, the additive include with
Lower raw material：Traditional Chinese drug mixture, corn protein powder, soybean lecithin, folic acid, lutein, fish multiannensional-sine, lysine, threonine,
The raw material that ferric sulfate, zinc sulfate, fructooligosaccharide, lactobacillus plantarum, bacillus subtilis and amylase, wherein Traditional Chinese drug mixture include
Medicinal material has：Divine Comedy, Radix Astragali, Radix Paeoniae Alba, dark plum, wild rice rice, white fungus, purple perilla, cortex trichosanthis, cynomorium songaricum, Schisandra chinensis, wilsonii, umbellate pore furgus, fish raw meat
Grass, Verbena officinalis, gynostemma pentaphylla, the stem of noble dendrobium, Chinese lobelia and sowthistle.It is also needed other than medicinal herb components in the feed addictive prescription
Other non-medicinal herb components are added so that the cost of feed addictive is higher, is not easy to large-scale promotion use.
One kind is disclosed in CN106361955A using profitable strain fermentation of Chinese herbal medicine as feed addictive to prevent fishes and shrimps
A kind of method of bacterial disease.The Chinese herbal medicine include Radix Astragali, Radix Codonopsis, cactus, coptis root, radix scutellariae, Radix Isatidis, cape jasmine, Herba Andrographitis,
Eucalyptus robusta, rhizoma anemarrhenae, the root bark of tree peony, campanulaceae, raw hawthorn, root of Aucklandia lappa Decne etc..Said herbal medicine is selected, crushes, impregnates, decocting, fermenting and make
It must prevent the feed addictive of fishes and shrimps bacterial disease, the use of effective prebiotic strain be mainly during the fermentation Te Jila gemma bars
Bacterium, Streptomyces violaceus, cloth Laplace saccharomycete, lactobacillus bulgaricus, Rhodopseudomonas spheroides etc..It is prepared by the feed addictive
It needs to ferment in Chinese herbal medicine in journey, preparation process is complicated.
Invention content
It is an object of the invention to a kind of fishes and shrimps feed addictives and its preparation method and application.
The technical solution used in the present invention is：
A kind of fishes and shrimps feed addictive, contains：Herba Verbenae, Sagina subulata extract, Flos Eriocauli extract, long-noded pit viper
Diffusa extract, Herba Agrimoniae extract, Geranium extract, Herba Pyrolae extract.
Further, the parts by weight of each ingredient are in additive：20-40 parts of Herba Verbenae, Sagina subulata extract
20-40 parts, 20-40 parts of Flos Eriocauli extract, 20-40 parts of herba hedyotis diffusae extract, 20-40 parts of Herba Agrimoniae extract, old stork
Careless extract 20-40 parts, 20-40 parts of Herba Pyrolae extract.
Further, the parts by weight of each ingredient are in additive：25-35 parts of Herba Verbenae, Sagina subulata extract
25-35 parts, 25-35 parts of Flos Eriocauli extract, 25-35 parts of herba hedyotis diffusae extract, 25-35 parts of Herba Agrimoniae extract, old stork
Careless extract 25-35 parts, 25-35 parts of Herba Pyrolae extract.
Further, the preparation method of additive is：It chooses and examines qualified Chinese medicinal material extract raw material, use Ultramicro-powder
Broken machine crushes raw material, and the raw material after crushing are placed in mixing vessel in proportion, is uniformly mixed.
A kind of fishes and shrimps mixed feed contains above-mentioned fishes and shrimps feed addictive.
Further, the additive amount of fishes and shrimps feed addictive is 0.1%~1%.
Further, the additive amount of fishes and shrimps feed addictive is 0.2%~0.6%.
Further, the preparation method of fishes and shrimps mixed feed is：Fishes and shrimps feed addictive and feed adhesive are mixed equal
It is even, then with basal feed in blender mixing, spray moisture so that fishes and shrimps feed addictive is uniformly bonded in feed surface layer,
Freeze-drying.
Further, fishes and shrimps feed addictive and feed adhesive mixing quality ratio are 1:1.
Further, fishes and shrimps mixed feed is seawater carp compound feed or prawn mixed feed.
The beneficial effects of the invention are as follows：
The fishes and shrimps feed addictive of the present invention is pure plant component, is free of industrial chemicals, is reduced because industrial chemicals pollutes
Caused risk not only improves the prevention of damage by disease of fish, shrimp using the feed addictive of the present invention, improves survival rate, can also
The digestive ferment secretion level for improving fish, shrimp promotes to effectively facilitate nutrient digesting and assimilating in fishes and shrimps body and convert
The healthy growth of fish, shrimp, thus can be substantially improved fish, shrimp aquaculture industry economic benefit, promote the sound development of industrial chain.Through
Verification experimental verification, the additive added in feed in the present invention can effectively prevent grouper, black porgy, culture of Penaeus vannamei process
Middle disease problem contributes to the further development of fish, shrimp culture industry.
Specific implementation mode
Below in conjunction with embodiment, the present invention is further explained, it should be appreciated that following embodiment be merely to illustrate the present invention and
It is not used in and limits the scope of the invention.
Basic fish feed used in the embodiment of the present invention is RUBY board grouper compound feeds, product standard number：
Q/GDYQS 07-2015；The basic seawater fish feed used is hippocampus board fish buoyancy expanding compound feed, product standard
Number：Q/FZHM005；Basic shrimp mixed feed is positive famous prawn mixed feed, product standard number：Q/MFDF0 07-2015,
Used Chinese medicinal material extract raw material is conventional Chinese medicine material purchased in market.
Involved in embodiment to data be calculated by the following formula：
Survival rate (SR, %)=100 × end end is at live fish (shrimp) mantissa/initial fish (shrimp) mantissa；
The long growth rate of body (BGR, %)=100 × (end body length-initial body is long eventually)/initial body is long；
Body weight growth rate (WGR, %)=100 × (the initial equal weight of end weight-eventually)/initial equal weight；
Feed coefficient (FC)=feedstuff feeding amount/(whole opisthosoma weight-original body mass).
Embodiment 1
A kind of fishes and shrimps feed addictive, is counted in parts by weight, wherein containing 25 parts of Herba Verbenae, Sagina subulata extract
35 parts, 25 parts of Flos Eriocauli extract, 35 parts of herba hedyotis diffusae extract, 25 parts of Herba Agrimoniae extract, 35 parts of Geranium extract,
30 parts of Herba Pyrolae extract.
Take and examine qualified above-mentioned Chinese medicinal material extract raw material, crushed using micronizer (90% up to 300 mesh), by than
Example is uniformly mixed in V-mixer to obtain the final product.
Embodiment 2
A kind of fishes and shrimps feed addictive, is counted in parts by weight, wherein containing 35 parts of Herba Verbenae, Sagina subulata extract
25 parts, 35 parts of Flos Eriocauli extract, 25 parts of herba hedyotis diffusae extract, 35 parts of Herba Agrimoniae extract, 25 parts of Geranium extract,
30 parts of Herba Pyrolae extract.
Take and examine qualified above-mentioned Chinese medicinal material extract raw material, crushed using micronizer (90% up to 300 mesh), by than
Example is uniformly mixed in V-mixer to obtain the final product.
Embodiment 3
A kind of fishes and shrimps feed addictive, is counted in parts by weight, wherein containing 30 parts of Herba Verbenae, Sagina subulata extract
30 parts, 32 parts of Flos Eriocauli extract, 28 parts of herba hedyotis diffusae extract, 30 parts of Herba Agrimoniae extract, 25 parts of Geranium extract,
35 parts of Herba Pyrolae extract.
Take and examine qualified above-mentioned Chinese medicinal material extract raw material, crushed using micronizer (90% up to 300 mesh), by than
Example is uniformly mixed in V-mixer to obtain the final product.
Embodiment 4
A kind of fishes and shrimps feed addictive, is counted in parts by weight, wherein containing 32 parts of Herba Verbenae, Sagina subulata extract
28 parts, 30 parts of Flos Eriocauli extract, 30 parts of herba hedyotis diffusae extract, 35 parts of Herba Agrimoniae extract, 30 parts of Geranium extract,
25 parts of Herba Pyrolae extract.
Take and examine qualified above-mentioned Chinese medicinal material extract raw material, crushed using micronizer (90% up to 300 mesh), by than
Example is uniformly mixed in V-mixer to obtain the final product.
Embodiment 5
A kind of fishes and shrimps feed addictive, is counted in parts by weight, wherein containing 30 parts of Herba Verbenae, Sagina subulata extract
30 parts, 30 parts of Flos Eriocauli extract, 30 parts of herba hedyotis diffusae extract, 30 parts of Herba Agrimoniae extract, 30 parts of Geranium extract,
30 parts of Herba Pyrolae extract.
It takes and examines qualified Chinese medicinal material extract raw material, (90% up to 300 mesh) is crushed using micronizer, is existed in proportion
It is uniformly mixed in V-mixer to obtain the final product.
Embodiment 6
A kind of grouper compound feed, wherein the basal feed containing mass fraction 99.6% and 0.2% embodiment 1 in
Additive.
By additive with 1:1 ratio and alphalise starch mixing, then mixing, spray 4~5% in blender with basal feed
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 7
A kind of grouper compound feed, wherein the basal feed containing mass fraction 99.2% and 0.4% embodiment 2 in
Additive.
By additive with 1:1 ratio and sodium alginate mixing, then mixing, spray 4~5% in blender with basal feed
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 8
A kind of grouper compound feed, wherein the basal feed containing mass fraction 98.8% and 0.6% embodiment 3 in
Additive.
By additive with 1:1 ratio and melon draw glue mixing, then with basal feed in blender mixing, spray 4~5%
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 9
A kind of marine fish culture feed, wherein the basal feed containing mass fraction 98.8% and 0.6% embodiment 4 in
Additive.
By additive with 1:1 ratio and alphalise starch mixing, then mixing, spray 4~5% in blender with mixed feed
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 10
A kind of prawn mixed feed, wherein the basal feed containing mass fraction 99.6% and 0.2% embodiment 1 in
Additive.
By additive with 1:1 ratio and alphalise starch mixing, then mixing, spray 4~5% in blender with basal feed
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 11
A kind of prawn mixed feed, wherein the basal feed containing mass fraction 99.2% and 0.4% embodiment 2 in
Additive.
By additive with 1:1 ratio and sodium alginate mixing, then mixing, spray 4~5% in blender with basal feed
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
Embodiment 12
A kind of prawn mixed feed, wherein the basal feed containing mass fraction 98.8% and 0.6% embodiment 3 in
Additive.
By additive with 1:1 ratio and melon draw glue mixing, then with basal feed in blender mixing, spray 4~5%
Moisture so that additive is uniformly bonded in feed surface layer, is freeze-dried to obtain the final product.
One, fish disease-resistant growth-promoting ability detects
(1), influence of the fishes and shrimps feed addictive to epinephelus lanceolatus fish growth and disease resisting effect is added in feed
This experiment is divided into 4 groups using epinephelus lanceolatus fish as subjects, and each group situation is as follows, and A groups are control group, B, C, D
Group is test group：
1. epinephelus lanceolatus fish test of table is grouped
| Grouping | A groups | B groups | C groups | D groups |
| Body grows (cm) | 17.01±0.09 | 17.14±0.06 | 16.49±0.43 | 16.64±0.15 |
| Weight (g) | 87.05±2.24 | 85.54±0.83 | 82.91±1.80 | 82.79±1.7 |
| Initial mantissa's (tail) | 150 | 150 | 150 | 150 |
By tetra- groups of A, B, C, D, each group set 3 it is parallel, normal farm pond in industrial circulating water cultivating system into
Row feeds experiment, and A is that control group feeds basal feed, and B groups feed the grouper feed in embodiment 6, and C groups feed implementation
Grouper feed in example 7, D groups feed the grouper feed in embodiment 8, and the trophic level of feed is：Thick egg
White is 49.17%, and crude fat 10.35%, breeding cycle is 56 days, morning 9:00 and afternoon 17:00 by fish body weight 1%~
2% is quantitatively fed, standardized administration, whole sea-farming, and water quality index meets national standard, pH value 7.2-8.6, dissolved oxygen 5-
7mg/L, 23-32 DEG C of water temperature.Periodically water quality is monitored, including pH, dissolved oxygen, water temperature etc..Test result is as shown in table 2.
The growth data and biochemical indicator of each test group of 2. grouper of table and control group
| Grouping | A groups | B groups | C groups | D groups |
| First initial body length (cm) | 17.01±0.09 | 17.14±0.06 | 16.49±0.43 | 16.64±0.15 |
| Original body mass (g) | 87.05±2.24 | 85.54±0.83 | 82.91±1.80 | 82.79±1.7 |
| Whole opisthosoma grows (cm) | 20.68±0.98 | 21.57±0.89 | 20.91±0.78 | 20.89±0.85 |
| Whole opisthosoma weight (g) | 151.11±2.45 | 174.11±3.45 | 162.86±2.15 | 159.70±3.12 |
| The long growth rate of body (%) | 21.58 | 25.84 | 26.80 | 25.62 |
| Body weight growth rate (%) | 73.59 | 103.54 | 96.45 | 92.87 |
| Feed coefficient | 1.38 | 1.20 | 1.30 | 1.20 |
| Grazing rate % | 2.04±0.13a | 2.34±0.12ab | 2.22±0.15a | 2.18±0.14a |
| Muscle crude protein (%) | 21.64±0.05a | 21.4±0.08a | 22.3±0.12ab | 23.13±0.09b |
| Muscle crude fat (%) | 5.24±0.02b | 5.1±0.03ab | 4.75±0.01a | 4.56±0.02a |
| Survival rate rate (%) | 95.83 | 96.39 | 96.67 | 99.17 |
| Lysozyme activity | 322.64±7.30a | 324.76±5.19a | 479.16±2.19b | 358.09±3.37a |
| Lipase active (U/g*prot) | 172.12±1.46a | 170.39±1.16a | 175.34±1.28a | 181.20±1.57b |
| Tryptic activity (U/mg*prot) | 1268.18±3.65a | 1397.35±3.75b | 1244.12±4.19a | 1317.33±4.40a |
As shown in Table 2, the grazing rate of B groups grouper is 2.34 ± 0.12bIt is significantly higher than control group 2.04 ± 0.13a(P<
0.05)；The survival rate (96.39%) of B group groupers is higher than control group (95.83%), and the long growth rate of body (25.84%) is higher than
Control group (21.58%), body weight growth rate (96.45%) are significantly higher than control group (73.59%) (P<0.05), feed coefficient
(1.20) it is less than control group (1.38), display plays the role of good growth promoting using additive and ingests and grow, to the stone of raising
Spot fish sampling Detection serum lysozyme activities reached, the lysozyme activity (324.76 ± 5.19 of B groupsa) higher than control group (322.64 ±
7.30a) (P > 0.05), illustrate the disease resisting effect for having certain；Measure the albumen and fat in the fresh muscle samples of fish, B group fleshes
The protein content (21.4 ± 0.08 of meata), the protein content of fat content (5.1 ± 0.03ab) and control group (21.64 ±
0.05a), fat content (5.24 ± 0.02b) quite, be not significantly different, illustrate additive will not change fish nutritional ingredient and
Quality；Digestive ferment (lipase active, tryptic activity) active quantities, the lipase activity of B groups and control group when off-test
Property (170.39 ± 1.16aWith 172.12 ± 1.46a) maintain an equal level, but B groups tryptic activity (1397.35 ± 3.75b) be significantly higher than
Control group (1268.18 ± 3.65a), it matches with the corresponding growth result of B groups and control group, the former is more than the latter, into one
Step illustrates that the additive has the effect for promoting to grow.
The grazing rate of C group groupers is 2.22 ± 0.15aHigher than control group 2.04 ± 0.13a(P ﹥ 0.05)；C group groupers
Survival rate (96.67%) be higher than control group (95.83%), the long growth rate of body (26.80%) higher than control group (21.58%),
Body weight growth rate (103.54%) is significantly higher than control group (73.59%) (P<0.05), feed coefficient (1.30) is less than control group
(1.38), display is had using additive promotes to ingest well and promotes growth；To the grouper sampling Detection blood of raising
Clear lysozyme activity, the lysozyme activity (479.16 ± 2.19 of C groupsb) it is significantly higher than control group (322.64 ± 7.30a)(P<
0.05), display has good disease-resistant growth-promoting to act on using 0.4% additive；Measure the fresh sample muscle protein and fat of fish
Fat, the protein content (22.3 ± 0.12 of C group muscleab), fat content (4.75 ± 0.01a) with the protein content of control group
(21.64±0.05a) compare, protein content increases, fat content (5.24 ± 0.02b) significantly reduce, illustrate additive
Play the role of centainly improving grouper nutritional ingredient and quality；Digestive ferment (lipase active, tryptose enzyme activity when off-test
Property) active quantities, the lipase active (175.34 ± 1.28 between C groups and control groupaWith 172.12 ± 1.46a) maintain an equal level, C groups
Tryptic activity 1244.12 ± 4.19aWith control group 1268.18 ± 3.65aAlso quite.
The grazing rate of D group groupers is 2.18 ± 0.14aHigher than control group 2.04 ± 0.13a(P ﹥ 0.05)；D group groupers
Survival rate (99.17%) be higher than control group (95.83%), the long growth rate of body (25.62%) higher than control group (21.58%),
Body weight growth rate (92.87%) is significantly higher than control group (73.59%) (P<0.05), feed coefficient (1.20) is less than control group
(1.38), display using additive play the role of certain promotions ingest and well promote grow；The grouper of raising is taken out
Sample detects serum lysozyme activities reached, the lysozyme activity (358.09 ± 3.37 of D groupsa) it is higher than control group (322.64 ± 7.30a)(P
> 0.05), display has certain resistant effect using 0.6% additive；The fresh sample muscle protein and fat of fish are measured,
The protein content (23.13 ± 0.09 of D group muscleb), fat content (4.56 ± 0.02a) with the protein content (21.64 of control group
±0.05a) compare, protein content significantly improves (P<0.05), fat content (5.24 ± 0.02b) significantly reduce (P<0.05),
Illustrate play the role of centainly improving the nutritional ingredient and quality of grouper using 0.6% additive；Digestive ferment (fat when off-test
Fat enzymatic activity, tryptic activity) active quantities, the lipase active (181.20 ± 1.57 between D groups and control groupbWith
172.12±1.46a) significantly increase (P<0.05), C groups tryptic activity (1317.33 ± 4.40a) and control group (1268.18
±3.65a) compared to raising.
It can be seen from the above result that the feed addictive during addition is of the invention in proportion in basal feed has grouper
The effect of disease-resistant growth-promoting.
(2), influence of the fishes and shrimps feed addictive to black porgy growth and disease resisting effect is added in feed
This experiment is divided into 2 groups using black porgy as subjects, and each group situation is as follows, and A groups are control group, and B groups are experiment
Group：
The experiment grouping of 3. black porgy of table
To be set in two groups, each group of A, B 3 it is parallel, the normal farm pond in industrial circulating water cultivating system carries out
Experiment is fed, A is control group, feeds basal feed seawater carp compound feed (puffing material), and B groups feed the seawater in embodiment 9
The trophic level of fish feed, feed is：Crude protein is 44.35%, crude fat 5.86%, lysine 2.05%, raising week
Phase is 56 days, morning 9:00 and afternoon 17:00 is quantitatively fed by the 1%~2% of fish body weight, standardized administration, whole seawater
Cultivation, water quality index meet national standard, pH value 7.0-8.6, dissolved oxygen 5.5-7mg/L, 23-31 DEG C of water temperature.Periodically water quality is carried out
Monitoring, including pH, dissolved oxygen, water temperature etc..Test result is as shown in table 4.
The growth data and biochemical indicator of table 4. black porgy test group and control group
| Grouping | A groups | B groups |
| First initial body length (cm) | 9.16±0.19 | 9.57±0.26 |
| Original body mass (g) | 13.83±1.24 | 13.17±0.83 |
| Whole opisthosoma grows (cm) | 14.55±0.78 | 14.86±0.89 |
| Whole opisthosoma weight (g) | 53.79±0.85 | 60.01±1.15 |
| The long growth rate of body (%) | 48.99 | 52.09 |
| Body weight growth rate (%) | 301.59 | 324.96 |
| Survival rate (9%) | 95.0 | 100.0 |
| Feed coefficient | 1.002 | 1.079 |
| Muscle crude protein (%) | 15.2±0.16a | 15.6±0.11b |
| Muscle crude fat (%) | 3.19±0.04a | 3.44±0.09a |
| Lysozyme activity | 94.33±4.21a | 116.68±2.74b |
| Lipase active (U/g*prot) | 120.47±1.26a | 131.15±2.1b |
| Tryptic activity (U/mg*prot) | 948.36±3.24a | 994.72±3.06a |
According to table 4 it is found that B group black porgy survival rates (100%) are higher than control group (95.00%), the long growth rate of body
(52.09%) it is higher than control group (48.99%), body weight growth rate (324.96%) is significantly higher than control group (301.59%) (P<
0.05), feed coefficient (1.079) and control group (1.002) maintain an equal level, and display has good growth promoting function using additive；It is right
The black porgy sampling Detection serum lysozyme activities reached of raising, the lysozyme activity (116.68 ± 2.74 of B groupsb) it is significantly higher than control group
(94.33±2.12a)(P<0.05), illustrate there is good disease resisting effect；Measure the albumen and fat in the fresh muscle samples of fish
Fat, the protein content (15.66 ± 0.11 of B group muscleb), fat content (3.44 ± 0.09a) with the protein content of control group
(15.2±0.16a), fat content (3.19 ± 0.04a) comparison, the former is significantly increased, and the latter increases, without significance difference
It is different, illustrate that additive can improve the nutritional ingredient and quality of black porgy；Lipase active when off-test, B groups (131.15 ±
2.1b) it is significantly higher than the lipase active (120.47 ± 1.26 of control groupa)(P<, but B group tryptic activities 0.05)
(994.72±3.06a) though it is higher than control group (948.36 ± 3.24a), but not significantly.
Result above matches with the corresponding growth result of B groups and control group, the former is more than the latter, and further illustrating should
Fishes and shrimps feed addictive has growth promoting and disease resisting effect to black porgy.
Two, prawn disease-resistant growth-promoting ability detects
(1), the effect that Chinese medical extract grows Penaeus Vannmei shrimp seedling is added in feed
This experiment using white leg Shrimp as subjects, be divided into tri- groups, each group of A, B, C set 3 it is parallel, in work
Launch the white leg Shrimp for the health that body long size is 0.6-1.0cm in normal farm pond in factory's circulating water culture system
Seedling carries out feeding experiment, and A is control group, feeds basic prawn mixed feed, and B groups feed the cooperation of the prawn in embodiment 10 and raise
Material, C groups feed the cooperation of the prawn in embodiment 11 and raise, and the trophic level of feed is：Crude protein is 45.17%, and crude fat is
4.45%, lysine 2.35%, breeding cycle is 35 days, morning 6:00,10:00,12:00 and afternoon 15:00,18:00,22:
00 is quantitatively fed by the 0.5%~1% of shrimp weight, standardized administration, whole sea-farming, and water quality index meets national standard, pH
Value 7.2-8.6, dissolved oxygen 5-7mg/L, 23-32 DEG C of water temperature.Periodically water quality is monitored, including pH, dissolved oxygen, water temperature etc..It is real
Test that the results are shown in Table 5.
5. Penaeus Vannmei shrimp seedling culture experiment result of table counts
| Experiment grouping | A groups | B groups | C groups |
| Test water body (m3) | 40 | 40 | 40 |
| Throw seedling quantity (ten thousand tails) | 22.6 | 22.6 | 22.6 |
| Feeding time (d) | 35 | 35 | 35 |
| Harvesting specification (tail/500g) | 625 | 876 | 1260 |
| Harvesting shrimp seedling quantity (tail) | 57875 | 83745 | 101052 |
| Receive shrimp weight (kg) | 23.15 | 21.9 | 20.05 |
| Survival rate (%) | 25.61 | 37.06 | 44.71 |
| Feed coefficient | 1.303 | 1.378 | 1.355 |
| Superoxide dismutase | 36.86±1.06a | 51.23±1.57b | 46.58±1.62b |
| Lysozyme | 88.73±1.26a | 143.58±2.1b | 135.13±1.6b |
As shown in Table 5, the survival rate (37.06%) of B groups Penaeus Vannmei training seedling is significantly higher than control group (25.61%), B
Group improves 44.71% than control group, and feed coefficient (1.378) maintains an equal level with control group (1.303)；To the Penaeus Vannmei of raising
The lysozyme activity (LEZ) of sampling Detection musculature, the lysozyme activity (143.58 ± 2.1 of B group prawnsb) be significantly higher than pair
According to group (88.73 ± 1.26a)(P<0.05)；To the superoxide dismutase of the Penaeus Vannmei sampling Detection musculature of raising
Vigor (SOD), the superoxide dismutase activity (51.23 ± 1.57 of B group prawns tissueb) be significantly higher than control group (36.86 ±
1.06a)(P<0.05).Result above matches with the corresponding survival rate result of B groups and control group, the former is more than the latter.
The survival rate (44.71) of C group Penaeus Vannmeis training seedling is significantly higher than control group (25.61%), and C groups are carried than control group
High by 74.58%, feed coefficient (1.355) maintains an equal level with control group (1.303)；To its flesh of the Penaeus Vannmei sampling Detection of raising
The lysozyme activity (LEZ) of meat tissue, the lysozyme activity (135.13 ± 1.6 of B group prawnsb) it is significantly higher than control group (88.73
±1.26a)(P<0.05)；To the superoxide dismutase activity (SOD) of the Penaeus Vannmei sampling Detection musculature of raising,
The superoxide dismutase activity (46.58 ± 1.62 of C group Penaeus Vannmei tissuesb) be significantly higher than control group (36.86 ±
1.06a)(P<0.05).Result above matches with the corresponding survival rate result of C groups and control group, the former is more than the latter.
It these results suggest that the fishes and shrimps feed addictive has good disease resisting effect, test group survival rate can be made substantially
Degree improves.
(2), fishes and shrimps feed is added in feed add the effect grown to culture of Penaeus vannamei
This experiment using white leg Shrimp as subjects, be divided into two groups, each group of A, B set 3 it is parallel, it is outdoor right
Shrimp normal farm pond carries out feeding experiment, launches the healthy Penaeus Vannmei shrimp seedling that body long size is 3.5-4.5cm, and A is control
Group feeds basic prawn mixed feed, and B groups feed the prawn mixed feed in embodiment 12, and the trophic level of feed is：Thick egg
White is 42.17%, crude fat 4.35%, lysine 2.35%, and breeding cycle is 113 days, morning 6:00,12:00 and afternoon
18:00,22:00, it is quantitatively fed by the 0.5%~1.5% of shrimp weight, standardized administration, experimental condition：Whole seawater is supported
It grows, water quality index meets national standard, pH value 7.2-8.6, dissolved oxygen 5-7mg/L, 23-30 DEG C of water temperature.Experimental result is as follows：
6. Penaeus Vannmei growth test result of table counts
| Experiment grouping | A groups | B groups |
| Test area (mu) | 2 | 2 |
| Throw seedling quantity (tail) | 420000 | 400000 |
| Feeding time (day) | 113 | 113 |
| Harvesting specification (tail/500g) | 46 | 45 |
| Receive shrimp weight (kg) | 1315 | 1635 |
| Average yield per mu (kg/ mus) | 657.5 | 817.5 |
| Survival rate (%) | 28.8 | 36.78 |
| Feed coefficient | 1.8 | 1.5 |
| Sell shrimp price (member/500g) | 28 | 28 |
| Per mu yield value (ten thousand yuan) | 3.682 | 4.578 |
7. Penaeus Vannmei musculature superoxide dismutase testing result (SOD) of table
8. Penaeus Vannmei musculature alkaline phosphatase activities testing result (ACP) of table
9. Penaeus Vannmei musculature lysozyme activity (LEZ) of table
As shown in Table 6, the test group of fishes and shrimps feed addictive is added, the survival rate of Penaeus Vannmei obtains in actual production
To significantly improving, (absolute value improves 7.98%, 27.7%) relative value improves, and per mu yield improves 160kg, and per mu yield value improves 0.896 ten thousand
Member, feed coefficient lower 0.3 (saving feed cost 20%), have apparent economic benefit；It is right in vain by table 7,8,9 visible South America
The superoxide dismutase activity (SOD) of shrimp, alkaline phosphatase activities testing result (ACP), lysozyme activity (LEZ) test group
It is significantly higher than control group (P in mid-term, later stage<0.05), illustrate that adding Chinese medical extract has increase Penaeus Vannmei immunity
Effect with growth is promoted, shows as significantly improving for survival rate and increasing substantially for specific yield (per mu yield).
Claims (10)
1. a kind of fishes and shrimps feed addictive, it is characterised in that：Contain in additive：Herba Verbenae, Sagina subulata extract, paddy
Smart grass extract, herba hedyotis diffusae extract, Herba Agrimoniae extract, Geranium extract, Herba Pyrolae extract.
2. fishes and shrimps feed addictive according to claim 1, it is characterised in that：The parts by weight of each ingredient in additive
For：20-40 parts of Herba Verbenae, 20-40 parts of Sagina subulata extract, 20-40 parts of Flos Eriocauli extract, oldenlandia diffusa extraction
20-40 parts of object, 20-40 parts of Herba Agrimoniae extract, 20-40 parts of Geranium extract, 20-40 parts of Herba Pyrolae extract.
3. fishes and shrimps feed addictive according to claim 2, it is characterised in that：The parts by weight of each ingredient in additive
For：25-35 parts of Herba Verbenae, 25-35 parts of Sagina subulata extract, 25-35 parts of Flos Eriocauli extract, oldenlandia diffusa extraction
25-35 parts of object, 25-35 parts of Herba Agrimoniae extract, 25-35 parts of Geranium extract, 25-35 parts of Herba Pyrolae extract.
4. according to claims 1 to 3 any one of them fishes and shrimps feed addictive, it is characterised in that：The preparation method of additive
For：It chooses and examines qualified Chinese medicinal material extract raw material, crushed raw material using micronizer, in proportion by the original after crushing
Material is placed in mixing vessel, is uniformly mixed.
5. a kind of fishes and shrimps mixed feed, wherein containing Claims 1 to 4 any one of them fishes and shrimps feed addictive.
6. fishes and shrimps mixed feed according to claim 5, it is characterised in that：The additive amount of fishes and shrimps feed addictive is
0.1%~1%.
7. fishes and shrimps mixed feed according to claim 6, it is characterised in that：The additive amount of fishes and shrimps feed addictive is
0.2%~0.6%.
8. fishes and shrimps mixed feed according to claim 5, it is characterised in that：The preparation method of fishes and shrimps mixed feed is：It will
Fishes and shrimps feed addictive and feed adhesive are uniformly mixed, then with basal feed in blender mixing, spray moisture so that fish
Shrimp feed additive is uniformly bonded in feed surface layer, freeze-drying.
9. fishes and shrimps mixed feed according to claim 8, it is characterised in that：Fishes and shrimps feed addictive and feed adhesive are mixed
It is 1 to close mass ratio:1.
10. fishes and shrimps mixed feed according to claim 5, it is characterised in that：Fishes and shrimps mixed feed is that fish cooperation is raised
Material or prawn mixed feed.
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Application publication date: 20181113 |