CN105795112A - Special black sea bream immune enhancer and application thereof - Google Patents
Special black sea bream immune enhancer and application thereof Download PDFInfo
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- 239000003623 enhancer Substances 0.000 title abstract 5
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Abstract
The present invention discloses a special black sea bream immune enhancer and an application thereof. The special black sea bream immune enhancer is characterized by consisting of the following raw materials in parts by weight: 27-35 parts of vibrio alginolyticus lipopolysaccharides, 25-30 parts of yeast glucan, 20-25 parts of glycyrrhizin, 10-15 parts of flavobacterium odoratum and 5-13 parts of choline. The addition mass percentage of the immune enhancer in a black sea bream feed is 3-7%. The advantages are that the black sea bream swallowing percentage increases by 49.8%-201.8%, the swallowing index increases by 47.6%-400%, the lysozyme activity improves by 57.1%-342.8%, the survival rate improves by 3.36%-71.8%, and the immune enhancer significantly improves the pathogen resistance of the black sea bream, lowers the mortality caused by pathogenic bacteria, and enhances the immunity.
Description
Technical field
The present invention relates to the immunostimulant of a kind of aquatic fish, especially relate to a kind of black porgy special immune reinforcing agent and
Its application.
Background technology
Black porgy, belongs to Perciformes, Channa argus section, and Channa argus belongs to, because of its delicious meat, is of high nutritive value, China's southeastern coast and Japan,
The countries and regions such as Taiwan, Southeast Asia are very popular, are famous and precious marine fish of class.Black porgy belongs to omnivorousness, and vitality is strong, premunition
By force, relatively wide to the subject range of salinity and temperature, it is one of the improved seeds of sea-farming.In recent years, along with aquaculture collection
The raising day by day of reduction degree, various diseases take place frequently, and common disease has parasitic disease, such as trichodinasis；Bacterial disease, as
Vibriosis；And other diseases etc., black porgy aquaculture is caused impact greatly, governs its development.And traditional antibiotic and
Other drug is treated, and there are the various problems such as drug residue, environmental pollution, resistance, therefore the grinding of immunostimulant
Send out and come into one's own.Immunostimulant, it is possible to prevent virus diseases, strengthen aquatic animal to the resistivity of parasite, reduction condition
Mortality rate that pathogenic bacterium are caused, reduce the mortality rate of fry, improve the efficiency of antibiotic substance, strengthen the immunoprotection effect of vaccine
Really.At present, relevant report and patented invention about black porgy immunostimulant are less.
Summary of the invention
The technical problem to be solved is to provide a kind of black porgy resistance to cause of disease that can significantly improve, and reduces and causes
The mortality rate that pathogenic bacteria is caused, strengthens black porgy special immune reinforcing agent and the application thereof of its immunity, and this immunostimulant is at feedstuff
Middle interpolation is only 3%-7%, and black porgy percentage phagocytosis just can be made to improve 49.8%～201.8%, phagocytic index improve 47.6%～
400%, antalzyme activity improves 57.1%～342.8%, and survival rate improves 3.36%～71.8%.
The present invention solves the technical scheme that above-mentioned technical problem used: a kind of black porgy special immune reinforcing agent, by with
Lower raw material and parts by weight composition thereof: vibrio alginolyticus lipopolysaccharide 27-35, yeast dextran 25-30, glycyrrhizin 20-25, abnormal smells from the patient
Flavobacterium 10-15, choline 5-13.
Preferably it is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 35, yeast dextran 30, glycyrrhizin
25, flavobacterium odoratum 10, choline 5.
The application of above-mentioned black porgy special immune reinforcing agent, this immunostimulant interpolation mass percent in black porgy feedstuff
For 3-7%.
Compared with prior art, it is an advantage of the current invention that: the invention discloses a kind of black porgy special immune reinforcing agent and
Its application.In the formula of above-mentioned immunostimulant, vibrio alginolyticus lipopolysaccharide, is the constituent of vibrio alginolyticus cell wall, and molten
Algae vibrio is a kind of bacterial disease cause of disease that black porgy is common, adds vibrio alginolyticus lipopolysaccharide and can promote the increasing of black porgy B lymphocyte
Growing and break up, generating the immune protein IgM for vibrio alginolyticus, improving Immune efficiency, additionally vibrio alginolyticus lipopolysaccharide also is able to
Act on black porgy neutrophil and macrophage etc., strengthen the nonspecific immunity of black porgy.Yeast dextran, is yeast cells
The constituent of wall, glucosan can improve the activity of Fish neutrophil cell, mononuclear cell, macrophage and lysozyme, increases
The strong resistance to bacterial pathogens, can play the effect of enhancing to the humoral and cellular immune response of fish body, and yeast Portugal gathers
The inhibitory action of Vibrio vulnificus is especially apparent by sugar, and Vibrio vulnificus is also the pathogenic bacterium that black porgy is common.Glycyrrhizin, is a kind of
Saponin, aglycon is a class glucosides of triterpene or spirostane compounds, it is possible to strengthen phagocytic function, strengthens the anti-of fish body
Bacterium function, promotes or induces generation interferon, improving antalzyme activity, improving immunity and the premunition of black porgy.Abnormal smells from the patient Huang bar
Bacterium, a kind of probiotic bacteria, it is possible to the growth of suppression fish pathogenic entero becteria, improve the immunity of fish body, improve the resistance to virus,
And can promote to digest and assimilate nutrient substance, flavobacterium odoratum has good antagonism to vibrio, it is possible to prevention disease
Sick generation.The composition more than added is mainly for the immunostimulant of each disease of black porgy, and the most individually adds, and mixing adds
Add the effect that can play complementation, significantly improve the black porgy resistance to various cause of diseases, improve immunity.Choline, is good luring
Food agent, and also be able to produce impact on the immune system of Fish.
In sum, one black porgy special immune reinforcing agent of the present invention and application thereof, test result indicate that through inventor,
Under the coordinated effect of each composition, immunostimulant of the present invention adds only 3%-7% in feedstuff, just can make black porgy phagocytosis hundred
Proportion by subtraction improves 49.8%～201.8%, and phagocytic index improves 47.6%～400%, antalzyme activity improve 57.1%～
342.8%, survival rate improves 3.36%～71.8%, it is thus possible to significantly improve immunity and the premunition of black porgy, improves its survival
Rate.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
One, specific embodiment
A kind of black porgy special immune reinforcing agent, is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 27-35, ferment
Female glucosan 25-30, glycyrrhizin 20-25, flavobacterium odoratum 10-15, choline 5-13.Its preparation method: go above-mentioned former respectively
Material mixes in proportion, and is spray-dried.
Embodiment 1
A kind of black porgy special immune reinforcing agent, is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 27, yeast Portugal
Polysaccharide 25, glycyrrhizin 20, flavobacterium odoratum 15, choline 13.Its preparation method: take above-mentioned raw materials respectively and be mixed in proportion all
Even, it is spray-dried.
Embodiment 2
A kind of black porgy special immune reinforcing agent, is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 29, yeast Portugal
Polysaccharide 26, glycyrrhizin 22, flavobacterium odoratum 11, choline 11.Its preparation method: take above-mentioned raw materials respectively and be mixed in proportion all
Even, it is spray-dried.
Embodiment 3
A kind of black porgy special immune reinforcing agent, is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 30, yeast Portugal
Polysaccharide 28, glycyrrhizin 23, flavobacterium odoratum 12, choline 8.Its preparation method: take above-mentioned raw materials respectively and mix in proportion,
It is spray-dried.
Embodiment 4
A kind of black porgy special immune reinforcing agent, is made up of following raw material and parts by weight thereof: vibrio alginolyticus lipopolysaccharide 35, yeast Portugal
Polysaccharide 30, glycyrrhizin 25, flavobacterium odoratum 10, choline 5.Its preparation method: take above-mentioned raw materials respectively and mix in proportion,
It is spray-dried.
Two, Application Example
Test example
Breed variety: Xiangshan Bay nursery completes culture experiment in Xiangshan County of Ningbo City, that chooses that nursery hatches then is same
Criticizing black porgy seedling, average weight about 3.77 grams, body constitution is healthy and strong, juvenile fish 600 tail of anosis wound.It is divided into 5 test group, often organizes 3
Individual repetition, each repetition 40 tail, raise respectively in 15 identical seawater cages (1.5m × 1.5m × 1.5m), raise with basis
Material is supported 7 days temporarily, is allowed to adapt to environment.Normal feedstuff forms: imported fish meal 20.0%, bean cake 10.0%, casein 19.0%, flour
30.0%, import fish oil 6.22%, multidimensional 0.5%, many ore deposits 1.5%, choline chloride 0.2%, dalcium biphosphate 1.5%, cellulose
11.08%。
Aquaculture management: other cultivating conditions are suitable and identical, except that test group 1 is thrown something and fed adds 5% embodiment 1
The normal feedstuff of immunostimulant, test group 2 throws something and feeds and adds the normal feedstuff of 5% embodiment 2 immunostimulant,
Test group 3 is thrown something and fed and is added the normal feedstuff of 5% embodiment 3 immunostimulant, and test group 4 is thrown something and fed and added 5% embodiment 4
The normal feedstuff of immunostimulant, test group 5 is thrown something and fed normal feedstuff, with comparing.Every day throws something and feeds 2 times sooner or later, all uses and is satiated with food
The method for breeding thrown something and fed.Above-mentioned immunostimulant addition in normal feedstuff is alternatively 3%, 4%, 6% or 7%.
Results contrast: after cultivating 8 weeks, carries out salivary lysozyme mensuration and challenge viral dosage to each test group.
Salivary lysozyme sample collecting, each test group is randomly drawed 10 tail fishes, is carried out tail vein blood.Taken a blood sample
Liquid sample is divided into two parts: a with obtaining hemocyte after 0.5% sodium heparin anticoagulant, the thinnest for salivary lysozyme
The mensuration of born of the same parents' phagocytic activity；Another part method routinely collects serum, for the mensuration of the salivary lysozymes such as antibacterial activity.
Following analysis:
1, leukocytes phagocytic activity
With vibrio alginolyticus for phagocytosis thalline, with reference to the method mensuration blood leucocyte phagocytic activity to be measured of (1994) such as Siwicki,
Percentage phagocytosis (PP) and phagocytic index (PI) computing formula are as follows:
PP=(participating in cell number/100 of phagocytosis in 100 leukocyte) × 100
Sum/100 of PI=100 leukocyte endocytosis phage
Table 1 black porgy leukocyte at a time percentage phagocytosis and phagocytic index compare (%)
Note: the data in form are the mean+SD that every test group 3 repeats.
From table 1 it follows that in this moment, the leukocytes phagocytic percentage ratio of the black porgy in test group 1-4 and phagocytosis
Index is all substantially high than matched group (test group 5), improves compared to matched group percentage phagocytosis after adding black porgy immunostimulant
49.8%～201.8%, phagocytic index improves 47.6%～400%, illustrates that the black porgy leukocytes phagocytic testing 1-4 group is active
Apparently higher than matched group (test group 5).
2, antibacterial activity
With vibrio alginolyticus dry powder as substrate, with reference to Boman(1974) and (1980) method such as Hultmark measure test serum
Antibacterial activity, antibacterial activity (Ua) computing formula is as follows:
Antibacterial activity (Ua)=[(A0 – A)/A]1/2
Table 2 black porgy leukocyte at a time antibacterial activity measures
Note: the data in form are the mean+SD that every test group 3 repeats.
From Table 2, it can be seen that improve 57.1% compared to matched group antalzyme activity after adding black porgy immunostimulant
～342.8%, illustrate that the antibacterial activity of black porgy in test group 1-4 is all substantially high than matched group (test group 5).
3, challenge viral dosage
After 8 weeks respectively in the drum that each test group random choose 20 tail black porgy is disposed within (radius 0.8m, high about 1.2m), often
The vibrio alginolyticus suspension (6.2 × 10 of tail black porgy lumbar injection 0.3mL7CFU/mL), record counteracting toxic substances death condition in 10 days,
Calculate survival rate.Formula is as follows
Survival rate (SR, %)=(N0 – Nt)/N0×100
N0: for initial black porgy mantissa；Nt: for the dead final mantissa of black porgy.
Table 3 black porgy challenge viral dosage survival rate (%)
Note: the data in form are the mean+SD that every test group 3 repeats.
From table 3 it is observed that add after black porgy immunostimulant compared to matched group survival rate improve 3.36%～
71.8%, illustrate that the survival rate of black porgy in test group 1-4 is all substantially high than matched group (test group 5).
In summary, the non-specific immunity of the black porgy in test group 1-4 all significantly improving than matched group, this is described
Invention black porgy special immune reinforcing agent can significantly improve immunity and the premunition of black porgy, improves its survival rate etc..
Certainly, described above not limitation of the present invention, the present invention is also not limited to the example above.The art
Those of ordinary skill, in the essential scope of the present invention, the change made, is retrofited, adds or is replaced, and also should belong to the present invention's
Protection domain.
Claims (3)
1. a black porgy special immune reinforcing agent, it is characterised in that be made up of following raw material and parts by weight thereof: vibrio alginolyticus fat
Polysaccharide 27-35, yeast dextran 25-30, glycyrrhizin 20-25, flavobacterium odoratum 10-15, choline 5-13.
A kind of black porgy special immune reinforcing agent the most according to claim 1, it is characterised in that by following raw material and weight thereof
Number forms: vibrio alginolyticus lipopolysaccharide 35, yeast dextran 30, glycyrrhizin 25, flavobacterium odoratum 10, choline 5.
3. the application of the black porgy special immune reinforcing agent according to any one of claim 1-2, it is characterised in that: this immunity
Reinforcing agent interpolation mass percent in black porgy feedstuff is 3-7%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108783007A (en) * | 2018-07-20 | 2018-11-13 | 广东天地本草饲料科技股份有限公司 | A kind of fishes and shrimps feed addictive and its preparation method and application |
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| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
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| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
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| 张伟，等: ""溶藻弧菌脂多糖对大菱鲆免疫保护实验"", 《水产养殖》 * |
| 程忠刚，等: ""动物免疫增强剂的最新研究进展"", 《猪场保健》 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108783007A (en) * | 2018-07-20 | 2018-11-13 | 广东天地本草饲料科技股份有限公司 | A kind of fishes and shrimps feed addictive and its preparation method and application |
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Application publication date: 20160727 |