CN108746092A - A kind of cleaning method of liquid transfer gun head for elemental analysis - Google Patents
A kind of cleaning method of liquid transfer gun head for elemental analysis Download PDFInfo
- Publication number
- CN108746092A CN108746092A CN201810407790.3A CN201810407790A CN108746092A CN 108746092 A CN108746092 A CN 108746092A CN 201810407790 A CN201810407790 A CN 201810407790A CN 108746092 A CN108746092 A CN 108746092A
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- China
- Prior art keywords
- liquid transfer
- gun head
- transfer gun
- rinsed
- elemental analysis
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
- B08B3/12—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration by sonic or ultrasonic vibrations
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
Abstract
The invention discloses a kind of cleaning methods of the liquid transfer gun head for elemental analysis, include the following steps：It rinses liquid transfer gun head well solid residue in the tap water of flowing, octyl phenol polyoxyethylene ether cleaning solution is then added, impregnate 1~3h, be cleaned by ultrasonic 20~40min, take out, rinsed with tap water；Ethanol solution is added, 2~5h is impregnated, is rinsed with tap water after being cleaned by ultrasonic 20~40min；Then the mixed solution of nitric acid and sulfuric acid, soaked overnight is added, 20~60min of ultrasound at 30~40 DEG C is rinsed with tap water；It is finally rinsed with secondary water, naturally dry in dustfree environment is contained in clean pallet；Residue in liquid transfer gun head is removed, the measurement result of elemental analysis is not interfered with, used washing solution is prepared by the reagent chemicals that routine experimentation condition is easy to get, at low cost, is easy to implement；Avoid discarded liquid transfer gun head pollution on the environment.
Description
Technical field
The present invention relates to the cleaning fields of experimental apparatus, the more particularly to a kind of cleaning of liquid transfer gun head for elemental analysis
Method.
Background technology
Liquid-transfering gun is one kind of pipettor, is usually used in that laboratory is a small amount of or micro liquid pipettes, quick and convenient with its, smart
For the high advantage of density by the welcome of experimenter, the liquid-transfering gun of different size matches different size of pipette tips；General feelings
Under condition, liquid transfer gun head is a kind of consumables, disposable, and the raw material of general liquid transfer gun head is polyethylene or polytetrafluoroethyl-ne
Alkene is difficult to degrade in the natural environment, and chemical reagent is carried in the liquid transfer gun head in experiment, it is difficult to recycle.With
Our attention to new technology and quality monitoring, the quantity and scale in laboratory increasingly increase, with increasing for experimental amount, largely
Liquid transfer gun head discarded, prepare liquid transfer gun head and discarded liquid transfer gun head and prodigious pressure all generated to environment, processing is discarded
Liquid transfer gun head need to expend a large amount of manpower and materials.
As it can be seen that the prior art could be improved and improve.
Invention content
Place in view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of liquid reliefs for elemental analysis
The cleaning method of pipette tips, it is intended to solve to be finished for liquid transfer gun head a small amount of or micro, in trace analysis in the prior art i.e. useless
It abandons, it is difficult to it degrades in natural conditions, the big problem of the pressure caused by environment.
In order to achieve the above object, this invention takes following technical schemes：
A kind of cleaning method of liquid transfer gun head for elemental analysis, includes the following steps：
S001. it rinses liquid transfer gun head well solid residue in the tap water of flowing, octylphenol polyethylene ethylene oxide is then added
Ether cleaning solution impregnates 1~3h, is cleaned by ultrasonic 20~40min, takes out, is rinsed with tap water；
S002. ethanol solution is added into the liquid transfer gun head after step S001 cleanings, impregnates 2~5h, is cleaned by ultrasonic 20~40min
It is rinsed afterwards with tap water；
S003. it is added the mixed solution of nitric acid and sulfuric acid into the liquid transfer gun head after step S002 cleaning, soaked overnight, 30~40
20~60min of ultrasound, is rinsed with tap water at DEG C；
S004. the liquid transfer gun head after step S003 cleanings is rinsed with secondary water, is contained in dustfree environment certainly with clean pallet
So dry.
In the cleaning method of the liquid transfer gun head for elemental analysis, it is added into liquid transfer gun head in the step S001
2~3h is impregnated after octyl phenol polyoxyethylene ether cleaning solution, is cleaned by ultrasonic 20~30min.
In the cleaning method of the liquid transfer gun head for elemental analysis, the octylphenol polyethylene ethylene oxide in the step S001
The 1%~10% of the concentration of octyl phenol polyoxyethylene ether in ether cleaning solution.
In the cleaning method of the liquid transfer gun head for elemental analysis, after ethanol solution is added in the step S002,
3~4h is impregnated, is rinsed with tap water after being cleaned by ultrasonic 25~35min.
In the cleaning method of the liquid transfer gun head for elemental analysis, the step S002 is toward after step S001 cleanings
Liquid transfer gun head in be added potassium hydroxide-ethanol solution, be cleaned by ultrasonic 15~30min after, rinsed with tap water.
In the cleaning method of the liquid transfer gun head for elemental analysis, hydroxide in the potassium hydroxide-ethanol solution
The solubility of potassium is 0.5mol/L.
In the cleaning method of the liquid transfer gun head for elemental analysis, nitric acid and sulfuric acid are added in the step S003
Mixed solution, soaked overnight, 30~40min of ultrasound at 35 DEG C are rinsed with tap water.
In the cleaning method of the liquid transfer gun head for elemental analysis, the mixing of nitric acid and sulfuric acid in the step S003
The volume ratio of nitric acid and mixed solution is 1: 3 in solution, and the volume ratio of sulfuric acid and mixed solution is 1: 19.
In the cleaning method of the liquid transfer gun head for elemental analysis, liquid relief is rinsed with secondary water in the step S004
Pipette tips 3~5 times.
Advantageous effect：
The present invention provides a kind of cleaning methods of the liquid transfer gun head for elemental analysis, are coordinated by using different cleaning solutions
Different washing degree removes molecule of remaining solid residue, organic matter, element in liquid transfer gun head etc. one by one, ensures
Liquid transfer gun head after cleaning will not remaining influence determination of elemental analysis result impurity, used washing solution is by daily reality
The reagent chemicals that the condition of testing is easy to get are prepared, at low cost, are easy to implement；It is dirty caused by environment to avoid discarded liquid transfer gun head
Dye.
Description of the drawings
Fig. 1 is the flow chart of the cleaning method of the liquid transfer gun head for elemental analysis provided by the invention.
Specific implementation mode
The present invention provides a kind of cleaning method of the liquid transfer gun head for elemental analysis, to make the purpose of the present invention, technology
Scheme and effect are clearer, clear, and the present invention is described in more detail for the embodiment that develops simultaneously referring to the drawings.It should manage
Solution, described herein specific examples are only used to explain the present invention, is not intended to limit the present invention.
Referring to Fig. 1, the present invention provides a kind of cleaning method of the liquid transfer gun head for elemental analysis, including following step
Suddenly：
S001. it rinses liquid transfer gun head well solid residue in the tap water of flowing, octylphenol polyethylene ethylene oxide is then added
Ether cleaning solution impregnates 1~3h, is cleaned by ultrasonic 20~40min, takes out, is rinsed with tap water；
S002. ethanol solution is added into the liquid transfer gun head after step S001 cleanings, impregnates 2~5h, is cleaned by ultrasonic 20~40min
It is rinsed afterwards with tap water；
S003. it is added the mixed solution of nitric acid and sulfuric acid into the liquid transfer gun head after step S002 cleaning, soaked overnight, 30~40
20~60min of ultrasound, is rinsed with tap water at DEG C；
S004. the liquid transfer gun head after step S003 cleanings is rinsed with secondary water, is contained in dustfree environment certainly with clean pallet
So dry.
In elemental analysis, general liquid-transfering gun for sampling, transfer criteria solution or sample, addition reagent etc., in pipette tips
Residue is generally the organic matter that do not cleared up in the particle of sample, sample and element ion, molecule etc., passes through the above method
A variety of detergent of middle use match and the cleaning method of classification, can remove remaining impurity in above-mentioned liquid transfer gun head,
Realize liquid transfer gun head recycling, not only save experimental cost, but reduce preparation, processing liquid transfer gun head brought to environment
Pollution problem.
Preferably, in the step S001 into liquid transfer gun head be added octyl phenol polyoxyethylene ether cleaning solution after impregnate 2~
3h is cleaned by ultrasonic 20~30min；It is highly preferred that impregnating 3h after octyl phenol polyoxyethylene ether cleaning solution is added, it is cleaned by ultrasonic
30min；Octyl phenol polyoxyethylene ether have good wetability, and it is hydrophilic, lipophile is good, dirt-removing power is strong, can be preliminary
Entrained oiliness and aqueous residue in liquid transfer gun head after cleaning experiment.
Preferably, in the octyl phenol polyoxyethylene ether cleaning solution in the step S001 octyl phenol polyoxyethylene ether concentration
1%~10%；It is highly preferred that in the octyl phenol polyoxyethylene ether cleaning solution octyl phenol polyoxyethylene ether a concentration of 3%.Institute
The dirty removal capacities for stating the octyl phenol polyoxyethylene ether cleaning solution in concentration range disclosure satisfy that liquid transfer gun head in general element analysis
The cleaning with residue, not will produce after bath residual and introduce new pollution.
Preferably, after ethanol solution being added in the step S002,3~4h is impregnated, is cleaned by ultrasonic after 25~35min with certainly
Water is rinsed；It is highly preferred that after ethanol solution is added, 3h is impregnated, is rinsed with tap water after being cleaned by ultrasonic 30min；Ethanol solution
It can be effectively removed the organic matter of fatty, the sample middle part branch of elemental analysis carries the organic matter of fatty, uses shifting
Liquid rifle may make the organic matter that the fatty is remained in pipette tips, or the test solution after resolution when sampling, due to fatty
Organic matter more difficult resolution may have the case where not exclusively resolution after resolution processing, during being shifted with liquid-transfering gun,
Also it is easy to remain in liquid transfer gun head, is using ethanol solution to clean again after the washing of octyl phenol polyoxyethylene ether cleaning solution,
Further the substance can be cleaned up；Applicant sets above-mentioned cleaning procedure and is obtained by largely testing
Optimal cleaning effect.
Preferably, the step S002 is that addition potassium hydroxide-ethanol is molten in the liquid transfer gun head after step S001 cleanings
Liquid is rinsed after being cleaned by ultrasonic 15~30min with tap water；It is highly preferred that it is 20min to be cleaned by ultrasonic the time；If ethanol solution
It being also not enough in the case of cleaning up fatty organic matter, the effect cleaned using potassium hydroxide-ethanol solution is more preferable, and
When need not impregnate, working efficiency higher, but configure solution, it should be noted that the corrosivity of potassium hydroxide.
Preferably, the solubility of potassium hydroxide is 0.5mol/L in the potassium hydroxide-ethanol solution；The concentration not only meets
The requirement of cleaning will not cause the waste of drug again, and corrosivity will not be too strong；It is worth noting that, the potassium hydroxide-
In ethanol solution ethanol solution volatilize, with with with.
Preferably, it is added the mixed solution of nitric acid and sulfuric acid in the step S003, soaked overnight, ultrasound 30 at 35 DEG C~
40min is rinsed with tap water；It is highly preferred that ultrasonic time is 35min.
Preferably, the volume ratio of nitric acid and mixed solution is 1 in the mixed solution of nitric acid and sulfuric acid in the step S003:
3, the volume ratio of sulfuric acid and mixed solution is 1: 19.Nitric acid and sulfuric acid mixed solution under the concentration pass through above-mentioned immersion and cleaning
Time, the residues such as protein and metallic element ion can be removed effectively, test result is caused when avoiding reusing
It is abnormal.
Preferably, in the step S004 liquid transfer gun head is rinsed with secondary water 3~5 times；The impurity contained in secondary water from
Son is few, can effectively ensure that cleaning performance is not affected using secondary water in last cleaning procedure.
It, can according to the technique and scheme of the present invention and its hair it is understood that for those of ordinary skills
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect range.
Claims (9)
1. a kind of cleaning method of liquid transfer gun head for elemental analysis, which is characterized in that include the following steps：
S001. it rinses liquid transfer gun head well solid residue in the tap water of flowing, octylphenol polyethylene ethylene oxide is then added
Ether cleaning solution impregnates 1~3h, is cleaned by ultrasonic 20~40min, takes out, is rinsed with tap water；
S002. ethanol solution is added into the liquid transfer gun head after step S001 cleanings, impregnates 2~5h, is cleaned by ultrasonic 20~40min
It is rinsed afterwards with tap water；
S003. it is added the mixed solution of nitric acid and sulfuric acid into the liquid transfer gun head after step S002 cleaning, soaked overnight, 30~40
20~60min of ultrasound, is rinsed with tap water at DEG C；
S004. the liquid transfer gun head after step S003 cleanings is rinsed with secondary water, is contained in dustfree environment certainly with clean pallet
So dry.
2. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
2~3h is impregnated after octyl phenol polyoxyethylene ether cleaning solution is added into liquid transfer gun head in S001, is cleaned by ultrasonic 20~30min.
3. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
The 1%~10% of the concentration of octyl phenol polyoxyethylene ether in octyl phenol polyoxyethylene ether cleaning solution in S001.
4. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
After ethanol solution is added in S002,3~4h is impregnated, is rinsed with tap water after being cleaned by ultrasonic 25~35min.
5. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
Is potassium hydroxide-ethanol solution is added in the liquid transfer gun head after step S001 cleanings in S002, after being cleaned by ultrasonic 15~30min,
It is rinsed with tap water.
6. the cleaning method of the liquid transfer gun head according to claim 5 for elemental analysis, which is characterized in that the hydrogen-oxygen
The solubility for changing potassium hydroxide in potassium-ethanol solution is 0.5mol/L.
7. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
The mixed solution of nitric acid and sulfuric acid, soaked overnight are added in S003,30~40min of ultrasound at 35 DEG C is rinsed with tap water.
8. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
The volume ratio of nitric acid and mixed solution is 1: 3 in the mixed solution of nitric acid and sulfuric acid in S003, the volume of sulfuric acid and mixed solution
Than being 1: 19.
9. the cleaning method of the liquid transfer gun head according to claim 1 for elemental analysis, which is characterized in that the step
In S004 liquid transfer gun head is rinsed with secondary water 3~5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810407790.3A CN108746092B (en) | 2018-05-02 | 2018-05-02 | Method for cleaning pipette tip for element analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810407790.3A CN108746092B (en) | 2018-05-02 | 2018-05-02 | Method for cleaning pipette tip for element analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108746092A true CN108746092A (en) | 2018-11-06 |
| CN108746092B CN108746092B (en) | 2021-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810407790.3A Active CN108746092B (en) | 2018-05-02 | 2018-05-02 | Method for cleaning pipette tip for element analysis |
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Citations (7)
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|---|---|---|---|---|
| CN1271175A (en) * | 1999-03-26 | 2000-10-25 | 佳能株式会社 | Method for cleaning porous body and manufacture the same, non-porous film or keyed lining |
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2018
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|---|---|---|---|---|
| CN1271175A (en) * | 1999-03-26 | 2000-10-25 | 佳能株式会社 | Method for cleaning porous body and manufacture the same, non-porous film or keyed lining |
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| CN102019282A (en) * | 2009-09-16 | 2011-04-20 | 中国科学院电子学研究所 | Method for purifying ceramic-metal structural component |
| CN103949431A (en) * | 2014-04-28 | 2014-07-30 | 江苏双仪光学器材有限公司 | Golden lens cleaning process |
| CN106324714A (en) * | 2015-06-15 | 2017-01-11 | 丹阳市康恩宁医疗器械有限公司 | Cleaning method of resin lens |
| CN206688722U (en) * | 2017-05-23 | 2017-12-01 | 哈尔滨商业大学 | A kind of chemical test extracts transfer device with solution |
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