CN203981464U - A kind of Biological Samples for Electron is tissue treatment apparatus in batches - Google Patents
A kind of Biological Samples for Electron is tissue treatment apparatus in batches Download PDFInfo
- Publication number
- CN203981464U CN203981464U CN201420350072.4U CN201420350072U CN203981464U CN 203981464 U CN203981464 U CN 203981464U CN 201420350072 U CN201420350072 U CN 201420350072U CN 203981464 U CN203981464 U CN 203981464U
- Authority
- CN
- China
- Prior art keywords
- sample
- hand basket
- filter screen
- solution tank
- electron
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model provides a kind of Biological Samples for Electron batch processing device, by sample hand basket, handle, solution tank, groove lid, cell filter screen, pressure ring, formed, sample hand basket is provided with the little lattice of numbering that matrix is arranged, bottom is mesh structure, sample hand basket is placed in solution tank, cell filter screen is placed in little lattice, and its size is suitable mutually with the little lattice of numbering, and pressure ring is placed in cell filter screen bottom.The utility model is reasonable in design, make simple, cost of manufacture is cheap, during use, greatly save the experimental implementation time, can prepare different kind organism electron microscopic sample by mass, comprised the cell sample of massive texture sample and dispersion, reduce use cost, realize parallel laboratory test, feasibility is high, has fundamentally solved biological sample mass and has processed problem.
Description
Technical field
The utility model belongs to bioelectronics micrology field, relates to a kind of electron microscope biological sample batch processing device, is also applicable to have other processing that complexity is changed the sample of liquid step.
Background technology
Bioelectronics microscope is in biology, to study cell, most important, the conventional instrument of tissue hyper-microstructure.It occupies consequence in biological evolution, and the ultrastructure knowledge of current various cells, tissue is nearly all provided by it.
In bioelectronics micrology field, the preparation of biological sample is extremely crucial step, is one of basic fundamental of modern biology and medical science.The technology of preparing of biological electron microscope sample has directly determined sample quality, and its step complexity and cycle are long, comprises and draws materials, fixes, dewaters, permeates, and transmission electron microscope also comprises the operation of the meters such as embedding, polymerization 20 step left and right.When need sample size to be processed reaches some, need experimenter to spend a large amount of time and muscle power, and (electron microscopic sample is often minimum, easily loss to measure large rear easily increase error rate; Dehydration is changed liquid and is caused not in time dry or absorb airborne moisture content and affect the observations under Electronic Speculum).
For the problem of complicated and consuming time biological electron microscope sample making, lot of domestic and international laboratory and specialized vendor constantly release various innovations or modification method.Most representative is German Leica EMTP organized processing instrument, and sample preparation can realize full process automatization, but owing to bringing a high price (hundreds of thousands unit), and cannot process the cell sample of dispersion, and use is purchased in domestic rare laboratory.
The sample size that now every batch of many Electronic Speculum operating room that carry out external service needs to process reached dozens or even hundreds of, and economy and easy operating, be applicable to sample that Routine Test Lab adopts in batches preparation facilities but have no report.
Summary of the invention
The purpose of this utility model is to provide a kind of Biological Samples for Electron batch processing device, sample hand basket, handle, solution tank, groove lid, cell filter screen, pressure ring, consists of. and sample hand basket is provided with the little lattice that matrix is arranged, and little lattice have numbering; Sample hand basket bottom is mesh structure, and mesh size is less than conventional Electronic Speculum tissue sample diameter, can make solution smooth and easy mobile, makes again Electronic Speculum tissue sample can not drop out; Sample hand basket both sides are provided with handle, and sample hand basket is placed in solution tank, and solution tank size is slightly larger than sample hand basket, with groove lid, can prevent absorption of sample moisture, reagent volatilization etc.; The little lattice of the size of cell filter screen and sample hand basket band numbering are suitable mutually, are hydrophilic material with holes, make the conventional cell cannot be penetrating and solution can be freely penetrating, are exclusively used in to place to process the cell sample disperseing; The little lattice internal diameter of pressure ring external diameter and sample hand basket suits, and pressure ring is placed in cell filter screen bottom, for cell filter screen being pressed in to the little lattice of sample hand basket.
Sample hand basket, handle, solution tank, solution tank lid, the polypropylene pp that pressure ring material selection used does not react with solution used; The made hydrophilic nonwoven fabrics of cell filter screen material selection polypropylene used pp.
During use, calculate cell sample umber, in the corresponding little lattice of sample hand basket, place cell filter screen, with pressure ring, compress (as acellular sample can omit); In solution tank, pour the required reagent of first step into, sample hand basket is placed in to solution tank, adjust solution amount and make it higher than hand basket bottom liquid level higher than sample certain distance; Sample is placed in to mass sample hand basket, and the cell sample wherein disperseing is placed in cell personal module, carries out mark (noting keeping sample to be placed in corresponding solution, not dry); Build solution tank lid, be placed under temperature required condition; The time for the treatment of arrives, and changes solution in solution tank into next step required solution, step before permeating to pure embedding medium; Sample is taken out, and pure embedding medium embathes and is placed on room temperature infiltration in the embedding pipe that embedding medium is housed, polymerization; The utility model device cell filter screen is disposable use, reusable after remaining part washing.
The utility model is reasonable in design, makes simply, and cost of manufacture is cheap, during use, has greatly saved the real running time, can test mass and prepare different kind organism electron microscopic sample, comprises the cell sample of massive texture sample and dispersion, realizes parallel laboratory test, and feasibility is high.The utility model device, for Biological Samples for Electron batch processing, had both been applicable to tissue block sample, was also applicable to cell sample, had fundamentally solved biological sample mass and had processed problem.
Accompanying drawing explanation
Fig. 1 is the structural representation of device.
Fig. 2 is the structural representation of sample hand basket.
Fig. 3 is the structure schematic diagram of sample hand basket.
Fig. 4 is the structural representation of solution tank.
Fig. 5 is the structural representation of cell filter screen and pressure ring.
Fig. 6 is the embodiment 2 application Biological Samples for Electron prepared nephridial tissue samples of tissue treatment apparatus in batches.
Fig. 7 is the kidney Electronic Speculum ultra-thin section sample comparison of dyeing preparation, wherein schemes A application the utility model result, and figure B is application classic method result.
Embodiment
Below in conjunction with drawings and Examples, the utility model is further described.
embodiment mono-
Referring to Fig. 1-5, a kind of Biological Samples for Electron is tissue treatment apparatus in batches, and by sample hand basket 1, handle 2, solution tank 3, groove lid 4, cell filter screen 5 and pressure ring 6 form.Sample hand basket 1 is provided with the band of matrix arrangement and numbers little lattice, and the present embodiment sample hand basket 1 arranges 6*10 square little lattice, grid length of side 6mm; The high 10mm of sample hand basket; Sample hand basket 1 bottom is mesh structure, and mesh size is less than conventional Electronic Speculum tissue sample diameter, and the present embodiment is selected mesh size 0.3mm, make solution smooth and easy mobile, and Electronic Speculum tissue sample can not drop out; Sample hand basket 1 both sides are provided with handle 2, are convenient to carry take liquid; Sample hand basket 1 is placed in solution tank 3, the long 70mm of solution tank 3, and wide 45mm, high 20mm, with groove lid 4, can prevent absorption of sample moisture, reagent volatilization etc.The special-purpose filter screen 5 of cell is for placing cell sample, quantity is placed in the little lattice of sample hand basket 1 on demand, and the little lattice internal diameter of pressure ring 6 external diameters and sample hand basket suits, and pressure ring is placed in cell filter screen 5 bottoms, for cell filter screen 5 is pressed in to the little lattice of sample hand basket, this example pressure ring 6 external diameters are 5.2mm.
Sample hand basket 1, handle 2, solution tank 3, solution tank lid 4, the polypropylene pp that pressure ring 6 material selection used does not react with solution used, the made hydrophilic nonwoven fabrics of the special-purpose filter screen 5 material selection polypropylene used pp of cell.
During use, step is as follows:
(1) calculate cell sample umber, the special-purpose filter screen 5 of cell is positioned in the little lattice of corresponding sample hand basket by cell sample umber, with pressure ring 6, compress, as sample is tissue block, save this step;
(2) in solution tank 3, pour the required reagent of first step into;
(3) sample hand basket 1 is placed in to solution tank 3, adjusts solution amount and make it higher than sample hand basket 1 bottom, and higher than sample 3-4mm left and right;
(4) sample is placed in to the little lattice of sample hand basket 1, the cell sample of dispersion is drawn and is placed in cell personal module with pipettor, carries out mark (noting keeping sample to be placed in corresponding solution, not dry);
(5) build solution tank lid 4, be placed under temperature required condition;
(6) time for the treatment of arrives, and changes solution in solution tank 3 into next step required solution;
(7) repeat the 4th, 5 step operations, to acetone/embedding medium impregnation step;
(8) sample is taken out, pure embedding medium embathes and is placed on room temperature infiltration in the embedding pipe that embedding medium is housed, polymerization.
Reusable after the washing of the utility model device.The utility model device is for Biological Samples for Electron batch processing.
embodiment bis-
Use this Biological Samples for Electron batch processing device and traditional Biological Samples for Electron treating apparatus to process batch sample, step is as follows:
(1) use this Biological Samples for Electron tissue treatment apparatus in batches, with 60 Electronic Speculum biological specimen renal tissue pieces of embodiment 1 step process, as batch quantity group;
(2) use conventional process device eppendorf pipe or embedding pipe to process 60 Electronic Speculum biological specimen renal tissue pieces, as a control group;
(3) Electron microscopy comparison two prescription legal systems are made sample effect, the equal clear in structure of batch quantity group and control group, and resolution is high.Referring to Fig. 6, be the application Biological Samples for Electron prepared nephridial tissue sample of tissue treatment apparatus in batches, under Electronic Speculum, the characteristic filament of its amyloidosis is high-visible.
Fig. 7 applies respectively the kidney Electronic Speculum ultra-thin section sample that tissue treatment apparatus (A), classic method (B) dye and prepare in batches, and under Electronic Speculum, basilar memebrane electron dense thing structure is all high-visible.
(4) relatively two prescription legal systems are made handling time in each step of sample, and each step of batch quantity group relative comparison group is all saved a large amount of handling times, is beneficial to operation in enormous quantities.
Claims (3)
1. Biological Samples for Electron tissue treatment apparatus in batches, it is characterized in that, by sample hand basket (1), handle (2), solution tank (3), groove lid (4), cell filter screen (5) and pressure ring (6) form, sample hand basket (1) is provided with the band of matrix arrangement and numbers little lattice, sample hand basket (1) bottom is mesh structure, mesh size is less than conventional Electronic Speculum tissue sample diameter, sample hand basket (1) both sides are provided with handle (2), sample hand basket (1) is placed in solution tank (3), solution tank 3 is with groove lid (4), cell filter screen (5) is placed in little lattice, its size and sample hand basket (1) are provided with band that matrix arranges and number little lattice and fit mutually, pressure ring (6) is placed in cell filter screen (5) bottom.
2. a kind of Biological Samples for Electron batch tissue treatment apparatus according to claim 1, is characterized in that, the little lattice internal diameter of pressure ring (6) external diameter and sample hand basket suits.
3. a kind of Biological Samples for Electron according to claim 1 tissue treatment apparatus in batches, it is characterized in that sample hand basket (1), handle (2), solution tank (3), solution tank lid (4), the polypropylene pp that pressure ring (6) material selection used does not react with solution used; The made hydrophilic nonwoven fabrics of cell filter screen (5) material selection polypropylene used pp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420350072.4U CN203981464U (en) | 2014-06-29 | 2014-06-29 | A kind of Biological Samples for Electron is tissue treatment apparatus in batches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420350072.4U CN203981464U (en) | 2014-06-29 | 2014-06-29 | A kind of Biological Samples for Electron is tissue treatment apparatus in batches |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203981464U true CN203981464U (en) | 2014-12-03 |
Family
ID=51978895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420350072.4U Expired - Fee Related CN203981464U (en) | 2014-06-29 | 2014-06-29 | A kind of Biological Samples for Electron is tissue treatment apparatus in batches |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203981464U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104062160A (en) * | 2014-06-29 | 2014-09-24 | 浙江大学 | Electron microscope biologic sample batch tissue treatment device and application |
| CN106982566A (en) * | 2017-05-15 | 2017-07-28 | 山东棉花研究中心 | A kind of high flux sub-control cotton hair seed delinting device and method |
-
2014
- 2014-06-29 CN CN201420350072.4U patent/CN203981464U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104062160A (en) * | 2014-06-29 | 2014-09-24 | 浙江大学 | Electron microscope biologic sample batch tissue treatment device and application |
| CN106982566A (en) * | 2017-05-15 | 2017-07-28 | 山东棉花研究中心 | A kind of high flux sub-control cotton hair seed delinting device and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103327906B (en) | For realizing the method for collecting the device with transfer of molecules biological sample | |
| CN203981464U (en) | A kind of Biological Samples for Electron is tissue treatment apparatus in batches | |
| CN105865864B (en) | Cell block processing unit (plant) with quick preparation function | |
| CN104062160A (en) | Electron microscope biologic sample batch tissue treatment device and application | |
| Yang et al. | A fractal model for pressure drop through a cigarette filter | |
| CN203811612U (en) | Automatic analyzer for weighing and quantitative dilution of excrement | |
| CN202631843U (en) | Cell climbing slice special slide | |
| CN101858844A (en) | Agarose gel micro-flow chamber device for detecting cell migration | |
| CN207472610U (en) | Pretreatment system before a kind of water quality detection | |
| CN207703895U (en) | A kind of transmission electron microscope biological sample preparation facilities | |
| CN202735148U (en) | Disposable dyeing suit used for electron microscope ultrathin section | |
| CN105675375A (en) | Separating medium of inactivated nematode in dead masson pine wood and separation method and kit | |
| CN107790104B (en) | Heavy metal available state adsorbed film based on EDTA modified material and preparation method thereof | |
| CN113829459B (en) | Preparation method of artificially-degraded water-saturated wood | |
| CN203132980U (en) | Medical microscope counting pool | |
| CN203981474U (en) | A kind of Electronic Speculum ultra-thin section is dyeing apparatus in batches | |
| CN210376190U (en) | Electron microscope dyeing sample making devices | |
| CN209590056U (en) | A kind of slide loading attachment for cell settlement | |
| CN205091170U (en) | Portable biomedical sample TEM system appearance device | |
| CN203719999U (en) | Improved medical cell staining glass slide | |
| JP2017053700A (en) | Preparation method of sample for form observation, apparatus for liquid processing used therefor, and sample stand | |
| CN105259001B (en) | A kind of paraffin section pre-treating method for tissue biopsy | |
| CN106346798A (en) | Carbon fiber cloth composite material specimen winding forming device and forming method | |
| CN207703747U (en) | A kind of Biological Samples for Electron preparation facilities of efficient mixing liquid | |
| CN202149857U (en) | Tissue slice experiment dehydration bottle with grid device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 Termination date: 20190629 |