CN104062160A - Electron microscope biologic sample batch tissue treatment device and application - Google Patents
Electron microscope biologic sample batch tissue treatment device and application Download PDFInfo
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- CN104062160A CN104062160A CN201410297642.2A CN201410297642A CN104062160A CN 104062160 A CN104062160 A CN 104062160A CN 201410297642 A CN201410297642 A CN 201410297642A CN 104062160 A CN104062160 A CN 104062160A
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Abstract
The invention provides an electron microscope biologic sample batch tissue treatment device which consists of a sample basket, handles, a solution tank, a tank cover, cell filtering nets and pressing rings, wherein the sample basket is provided with numbered grids which are arranged in a matrix; the bottom of the sample basket is of a sieve structure; the sample basket is put into the solution tank; the cell filtering nets are arranged inside the grids; the sizes of the filtering nets are accordant with those of the numbered grids; the pressing rings are arranged at the bottoms of the filtering nets. The device is reasonable in design, convenient to manufacture and low in manufacturing cost, when the device is used, the experiment operation time is greatly shortened, various electron microscope biologic samples, including blocky tissue samples and dispersed cell samples, can be prepared in batches, the use cost is lowered, parallel experiments are achieved, very high feasibility is achieved, and the batch treatment of biologic samples is implemented substantially.
Description
Technical field
The invention belongs to bioelectronics micrology field, relate to a kind of electron microscope biological sample batch processing device, be also applicable to there are other processing that complexity is changed the sample of liquid step.
Background technology
Bioelectronics microscope is in biology, to study cell, most important, the conventional instrument of tissue hyper-microstructure.It occupies consequence in biological evolution, and the ultrastructure knowledge of current various cells, tissue is nearly all provided by it.
In bioelectronics micrology field, the preparation of biological sample is extremely crucial step, is one of basic fundamental of modern biology and medical science.The technology of preparing of biological electron microscope sample has directly determined sample quality, and its step complexity and cycle are long, comprises and draws materials, fixes, dewaters, permeates, and transmission electron microscope also comprises the operation of the meters such as embedding, polymerization 20 step left and right.When need sample size to be processed reaches some, need experimenter to spend a large amount of time and muscle power, and (electron microscopic sample is often minimum, easily loss to measure large rear easily increase error rate; Dehydration is changed liquid and is caused not in time dry or absorb airborne moisture content and affect the observations under Electronic Speculum).
For the problem of complicated and consuming time biological electron microscope sample making, lot of domestic and international laboratory and specialized vendor constantly release various innovations or modification method.Most representative is German Leica EMTP organized processing instrument, and sample preparation can realize full process automatization, but owing to bringing a high price (hundreds of thousands unit), and cannot process the cell sample of dispersion, and use is purchased in domestic rare laboratory.
The sample size that now every batch of many Electronic Speculum operating room that carry out external service needs to process reached dozens or even hundreds of, and economy and easy operating, be applicable to sample that Routine Test Lab adopts in batches preparation facilities but have no report.
Summary of the invention
For above-mentioned condition, the object of this invention is to provide a kind of Biological Samples for Electron batch processing device, for Biological Samples for Electron batch processing, be both applicable to tissue block sample, be also applicable to cell sample, fundamentally solve biological sample mass and processed problem.
A kind of Biological Samples for Electron batch processing of the present invention device, is comprised of sample hand basket, handle, solution tank, groove lid, cell filter screen, pressure ring. and sample hand basket is provided with the little lattice that matrix is arranged, and little lattice have numbering; Sample hand basket bottom is mesh structure, and mesh size is less than conventional Electronic Speculum tissue sample diameter, can make solution smooth and easy mobile, makes again Electronic Speculum tissue sample can not drop out; Sample hand basket both sides are provided with handle, and sample hand basket is placed in solution tank, and solution tank size is slightly larger than sample hand basket, with groove lid, can prevent absorption of sample moisture, reagent volatilization etc.; The little lattice of the size of cell filter screen and sample hand basket band numbering are suitable mutually, are hydrophilic material with holes, make the conventional cell cannot be penetrating and solution can be freely penetrating, are exclusively used in to place to process the cell sample disperseing; The little lattice internal diameter of pressure ring external diameter and sample hand basket suits, and pressure ring is placed in cell filter screen bottom, for cell filter screen being pressed in to the little lattice of sample hand basket.
Sample hand basket, handle, solution tank, solution tank lid, the polypropylene pp that pressure ring material selection used does not react with solution used; The made hydrophilic nonwoven fabrics of cell filter screen material selection polypropylene used pp.
Another object of the present invention is to provide the application of described device in Biological Samples for Electron batch processing, comprises the batch processing of tissue block sample and cell sample.
During use, calculate cell sample umber, in the corresponding little lattice of sample hand basket, place cell filter screen, with pressure ring, compress (as acellular sample can omit); In solution tank, pour the required reagent of first step into, sample hand basket is placed in to solution tank, adjust solution amount and make it higher than hand basket bottom liquid level higher than sample certain distance; Sample is placed in to mass sample hand basket, and the cell sample wherein disperseing is placed in cell personal module, carries out mark (noting keeping sample to be placed in corresponding solution, not dry); Build solution tank lid, be placed under temperature required condition; The time for the treatment of arrives, and changes solution in solution tank into next step required solution, step before permeating to pure embedding medium; Sample is taken out, and pure embedding medium embathes and is placed on room temperature infiltration in the embedding pipe that embedding medium is housed, polymerization; Apparatus of the present invention cell filter screen is disposable use, reusable after remaining part washing.
The present invention is reasonable in design, makes simply, and cost of manufacture is cheap, during use, has greatly saved the experimental implementation time, can prepare different kind organism electron microscopic sample by mass, comprises the cell sample of massive texture sample and dispersion, realizes parallel laboratory test, and feasibility is high.Apparatus of the present invention, for Biological Samples for Electron batch processing, reduce use cost, have both been applicable to tissue block sample, are also applicable to cell sample, have fundamentally solved biological sample mass and have processed problem.
Accompanying drawing explanation
Fig. 1 is the structural representation of device.
Fig. 2 is the structural representation of sample hand basket.
Fig. 3 is the structure schematic diagram of sample hand basket.
Fig. 4 is the structural representation of solution tank.
Fig. 5 is the structural representation of cell filter screen and pressure ring.
Fig. 6 is the embodiment 2 application Biological Samples for Electron prepared nephridial tissue samples of tissue treatment apparatus in batches.
Fig. 7 is the kidney Electronic Speculum ultra-thin section sample comparison of dyeing preparation, wherein schemes A application result of the present invention, and figure B is application classic method result.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.
embodiment mono-
Referring to Fig. 1-5, a kind of Biological Samples for Electron is tissue treatment apparatus in batches, and by sample hand basket 1, handle 2, solution tank 3, groove lid 4, cell filter screen 5 and pressure ring 6 form.Sample hand basket 1 is provided with the band of matrix arrangement and numbers little lattice, and the present embodiment sample hand basket 1 arranges 6*10 square little lattice, grid length of side 6mm; The high 10mm of sample hand basket; Sample hand basket 1 bottom is mesh structure, and mesh size is less than conventional Electronic Speculum tissue sample diameter, and the present embodiment is selected mesh size 0.3mm, make solution smooth and easy mobile, and Electronic Speculum tissue sample can not drop out; Sample hand basket 1 both sides are provided with handle 2, are convenient to carry take liquid; Sample hand basket 1 is placed in solution tank 3, the long 70mm of solution tank 3, and wide 45mm, high 20mm, with groove lid 4, can prevent absorption of sample moisture, reagent volatilization etc.The special-purpose filter screen 5 of cell is for placing cell sample, quantity is placed in the little lattice of sample hand basket 1 on demand, and the little lattice internal diameter of pressure ring 6 external diameters and sample hand basket suits, and pressure ring is placed in cell filter screen 5 bottoms, for cell filter screen 5 is pressed in to the little lattice of sample hand basket, this example pressure ring 6 external diameters are 5.2mm.
Sample hand basket 1, handle 2, solution tank 3, solution tank lid 4, the polypropylene pp that pressure ring 6 material selection used does not react with solution used, the made hydrophilic nonwoven fabrics of the special-purpose filter screen 5 material selection polypropylene used pp of cell.
During use, step is as follows:
(1) calculate cell sample umber, the special-purpose filter screen 5 of cell is positioned in the little lattice of corresponding sample hand basket by cell sample umber, with pressure ring 6, compress, as sample is tissue block, save this step;
(2) in solution tank 3, pour the required reagent of first step into;
(3) sample hand basket 1 is placed in to solution tank 3, adjusts solution amount and make it higher than sample hand basket 1 bottom, and higher than sample 3-4mm left and right;
(4) sample is placed in to the little lattice of sample hand basket 1, the cell sample of dispersion is drawn and is placed in cell personal module with pipettor, carries out mark (noting keeping sample to be placed in corresponding solution, not dry);
(5) build solution tank lid 4, be placed under temperature required condition;
(6) time for the treatment of arrives, and changes solution in solution tank 3 into next step required solution;
(7) repeat the 4th, 5 step operations, to acetone/embedding medium impregnation step;
(8) sample is taken out, pure embedding medium embathes and is placed on room temperature infiltration in the embedding pipe that embedding medium is housed, polymerization.
Reusable after apparatus of the present invention washing.Apparatus of the present invention are for Biological Samples for Electron batch processing.
embodiment bis-
Use this Biological Samples for Electron batch processing device and traditional Biological Samples for Electron treating apparatus to process batch sample, step is as follows:
(1) use this Biological Samples for Electron tissue treatment apparatus in batches, with 60 Electronic Speculum biological specimen renal tissue pieces of embodiment 1 step process, as batch quantity group;
(2) use conventional process device eppendorf pipe or embedding pipe to process 60 Electronic Speculum biological specimen renal tissue pieces, as a control group;
(3) Electron microscopy comparison two prescription legal systems are made sample effect, the equal clear in structure of batch quantity group and control group, and resolution is high.Referring to Fig. 6, be the application Biological Samples for Electron prepared nephridial tissue sample of tissue treatment apparatus in batches, under Electronic Speculum, the characteristic filament of its amyloidosis is high-visible.
Fig. 7 applies respectively the kidney Electronic Speculum ultra-thin section sample that tissue treatment apparatus (A), classic method (B) dye and prepare in batches, and under Electronic Speculum, basilar memebrane electron dense thing structure is all high-visible.
(4) relatively two prescription legal systems are made handling time in each step of sample, and each step of batch quantity group relative comparison group is all saved a large amount of handling times, is beneficial to operation in enormous quantities.
Claims (4)
1. a Biological Samples for Electron batch processing device, it is characterized in that, by sample hand basket (1), handle (2), solution tank (3), groove lid (4), cell filter screen (5) and pressure ring (6) form, sample hand basket (1) is provided with the band of matrix arrangement and numbers little lattice, sample hand basket (1) bottom is mesh structure, mesh size is less than conventional Electronic Speculum tissue sample diameter, sample hand basket (1) both sides are provided with handle (2), sample hand basket (1) is placed in solution tank (3), solution tank 3 is with groove lid (4), cell filter screen (5) is placed in little lattice, its size and sample hand basket (1) are provided with band that matrix arranges and number little lattice and fit mutually, pressure ring (6) is placed in cell filter screen (5) bottom.
2. a kind of Biological Samples for Electron batch processing device according to claim 1, is characterized in that, the little lattice internal diameter of pressure ring (6) external diameter and sample hand basket suits.
3. a kind of Biological Samples for Electron batch processing device according to claim 1, it is characterized in that sample hand basket (1), handle (2), solution tank (3), solution tank lid (4), the polypropylene pp that pressure ring (6) material selection used does not react with solution used; The made hydrophilic nonwoven fabrics of cell filter screen (5) material selection polypropylene used pp.
4. a kind of Biological Samples for Electron batch processing device according to claim 1 is applied in Biological Samples for Electron batch processing, and described biological sample is tissue block sample or cell sample.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410297642.2A CN104062160A (en) | 2014-06-29 | 2014-06-29 | Electron microscope biologic sample batch tissue treatment device and application |
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| CN201410297642.2A CN104062160A (en) | 2014-06-29 | 2014-06-29 | Electron microscope biologic sample batch tissue treatment device and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376045A (en) * | 2019-07-18 | 2019-10-25 | 株洲市中心医院 | A kind of experimental animal solid organ tissue infarct dyes fixed device and colouring method |
| WO2022222059A1 (en) * | 2021-04-21 | 2022-10-27 | 中国科学院深圳先进技术研究院 | Treatment apparatus |
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| EP0142575A1 (en) * | 1983-11-22 | 1985-05-29 | Freiherr von Gise, Hardo, Dr. med. | Incubation container for embedding biological samples in paraffin for use in automatic incubating and shaping devices |
| JPH09134693A (en) * | 1995-11-08 | 1997-05-20 | Kawasaki Steel Corp | Pretreatment method of nonconductive sample for observation with electron microscope, pretreating device and electron microscope |
| CN101778672A (en) * | 2007-08-02 | 2010-07-14 | 恰根有限公司 | Method and device for fixing/stabilising a sample |
| CN101788527A (en) * | 2010-03-09 | 2010-07-28 | 浙江大学 | Complete set of device for SCGE (single cell gel electrophoresis) test |
| CN202149857U (en) * | 2011-03-02 | 2012-02-22 | 上海海洋大学 | Tissue slice experiment dehydration bottle with grid device |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN203981464U (en) * | 2014-06-29 | 2014-12-03 | 浙江大学 | A kind of Biological Samples for Electron is tissue treatment apparatus in batches |
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2014
- 2014-06-29 CN CN201410297642.2A patent/CN104062160A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0142575A1 (en) * | 1983-11-22 | 1985-05-29 | Freiherr von Gise, Hardo, Dr. med. | Incubation container for embedding biological samples in paraffin for use in automatic incubating and shaping devices |
| JPH09134693A (en) * | 1995-11-08 | 1997-05-20 | Kawasaki Steel Corp | Pretreatment method of nonconductive sample for observation with electron microscope, pretreating device and electron microscope |
| CN101778672A (en) * | 2007-08-02 | 2010-07-14 | 恰根有限公司 | Method and device for fixing/stabilising a sample |
| CN101788527A (en) * | 2010-03-09 | 2010-07-28 | 浙江大学 | Complete set of device for SCGE (single cell gel electrophoresis) test |
| CN202149857U (en) * | 2011-03-02 | 2012-02-22 | 上海海洋大学 | Tissue slice experiment dehydration bottle with grid device |
| CN103267660A (en) * | 2013-04-19 | 2013-08-28 | 山东大学 | Pathological processing humidity box for cell slide |
| CN203981464U (en) * | 2014-06-29 | 2014-12-03 | 浙江大学 | A kind of Biological Samples for Electron is tissue treatment apparatus in batches |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376045A (en) * | 2019-07-18 | 2019-10-25 | 株洲市中心医院 | A kind of experimental animal solid organ tissue infarct dyes fixed device and colouring method |
| WO2022222059A1 (en) * | 2021-04-21 | 2022-10-27 | 中国科学院深圳先进技术研究院 | Treatment apparatus |
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Application publication date: 20140924 |