CN108721435A - A kind of Semen Cuscutae composite essential oil and the preparation method and application thereof - Google Patents

A kind of Semen Cuscutae composite essential oil and the preparation method and application thereof Download PDF

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CN108721435A
CN108721435A CN201810508882.0A CN201810508882A CN108721435A CN 108721435 A CN108721435 A CN 108721435A CN 201810508882 A CN201810508882 A CN 201810508882A CN 108721435 A CN108721435 A CN 108721435A
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essential oil
semen cuscutae
parts
composite
preparation
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劳永华
黄岳山
支晓兴
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South China University of Technology SCUT
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South China University of Technology SCUT
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/43—Cuscutaceae (Dodder family), e.g. Cuscuta epithymum or greater dodder
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    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
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    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
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Abstract

The invention belongs to the technical field of Chinese medicine preparation, a kind of Semen Cuscutae composite essential oil and the preparation method and application thereof is disclosed.The Semen Cuscutae composite essential oil mainly is extracted to obtain by the following raw material counted in parts by weight：500-700 parts of Semen Cuscutae, 300-500 parts of the fruit of Chinese wolfberry, 100-200 parts of raspberry, 60-100 parts of plantain seed, 50-80 parts of Schisandra chinensis.Method：(1) each raw material powder is mixed, is placed in supercritical fluid extraction kettle, is extracted with Co 2 supercritical fluid, obtain essential oil and residue；(2) it uses ethanol water that residue is carried out refluxing extraction, obtains extracting solution；(3) essential oil and extracting solution are mixed using supersonic spraying, obtains Semen Cuscutae composite essential oil.The Semen Cuscutae composite essential oil Transdermal absorption of the present invention is good, can treat impotence and seminal emission, enuresis frequent micturition etc., good effect；And composite essential oil external application, the various uncomfortable problems for reducing traditional Chinese medicine decocting and taking.

Description

A kind of Semen Cuscutae composite essential oil and the preparation method and application thereof
Technical field
The invention belongs to the technical fields of essential oil and traditional Chinese medicine for external application, and in particular to a kind of Semen Cuscutae composite essential oil and its Preparation method and application.The present invention composite essential oil can treat impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency rush down, vaginal bleeding due to deficiency of the kidney during pregnancy and Long infertility.
Background technology
The exploitation of Chinese medicine preparation is the important process of Chinese nation's Chinese medical culture development.When treating disease, especially exist When treating the diseases such as impotence and seminal emission, enuresis frequent micturition, spleen nephrasthenia diarrhea, vaginal bleeding due to deficiency of the kidney during pregnancy, Chinese medicine preparation is talked about, in typically taking orally Medicine preparation achievees the effect that treatment by oral.In Chinese medicine, due to a variety of medicinal materials be complexed and its in production process it is complicated Chemical environment results in compound drug action and is difficult to control and monitors；And by oral, some medicinal material impurity are it is also possible to meeting Not expected burden is caused to human liver, stomach, kidney etc. system；And by oral, have some Chinese medicines can not play compared with Good effect.In addition, medicinal material, due to the place of production and collecting season, condition of storage difference, the more difficult unification of quality and stabilization influence preparation It feeds intake, quality control and clinical efficacy.
It is rushed down to reach certain impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency, medical treatment in vaginal bleeding due to deficiency of the kidney during pregnancy and long infertility etc. Effect, the present invention are equipped with the fruit of Chinese wolfberry, raspberry, plantain seed, schisandra chinensis medicinal material using Semen Cuscutae as main material, are carried out by certain weight ratio Mixing extracts essential oil using supercritical liquid extraction technique, and extracts various flavones and polysaccharide work in conjunction with solvent reflux extraction Property substance extracting solution, Semen Cuscutae composite essential oil has been mixed and made into supersonic spraying.The composite essential oil absorbs fast, good effect.? In use, by composite essential oil for skin below navel, massage is sustained, and composite essential oil Transdermal absorption promotes active drug ingredient It without by digestive system, more quickly, directly, accurately by reproductive system absorbs, avoids extract oral via Digestive Various uncomfortable (adverse reactions of the non-principal component or heavy metal of such as medicinal material to liver, intestines, stomach etc.) of system.
Invention content
In order to overcome the shortcomings and deficiencies of the prior art, the purpose of the present invention is to provide a kind of Semen Cuscutae composite essential oils. The Semen Cuscutae composite essential oil of the present invention can treat impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency rush down, vaginal bleeding due to deficiency of the kidney during pregnancy and long infertility, Tool has a better effect and active drug ingredient can faster be absorbed.The present invention is by by composite essential oil external application, reaching and controlling The effect for the treatment of is different from the oral of traditional Chinese medicine, and avoiding extract oral, uncomfortable (such as medicinal material is not via digestive system various The adverse reaction to liver, intestines, stomach etc. such as principal component or heavy metal).
Another object of the present invention is to provide the preparation methods of above-mentioned Semen Cuscutae composite essential oil.The present invention is with Semen Cuscutae Main material is equipped with the fruit of Chinese wolfberry, raspberry, plantain seed, schisandra chinensis medicinal material, is mixed by certain weight ratio, uses supercritical fluid Abstraction technique extracts essential oil, and extracts various flavones and active polysaccharide substance extracting solution in conjunction with solvent reflux extraction, with ultrasound Spray-on process has been mixed and made into Semen Cuscutae composite essential oil.The preparation method of the present invention can make the effective of each medicinal material in composite essential oil Ingredient preferably plays drug effect.
It is still another object of the present invention to provide the applications of above-mentioned Semen Cuscutae composite essential oil.
The purpose of the present invention is achieved through the following technical solutions：
A kind of Semen Cuscutae composite essential oil mainly is extracted to obtain by the following raw material counted in parts by weight：Semen Cuscutae 500-700 Part, 300-500 parts of the fruit of Chinese wolfberry, 100-200 parts of raspberry, 60-100 parts of plantain seed, 50-80 parts of Schisandra chinensis；The extraction refers to Using essential oil in supercritical fluid extraction above-mentioned raw materials, then ethanol water is used to be returned the residue after extracting essential oil Stream extraction, the extracting solution of refluxing extraction is mixed with essential oil.
Raw material selected by the Semen Cuscutae composite essential oil further includes other raw materials, other raw materials are emblic, the fruit of glossy privet With more than one in the fruit of Cherokee rose.The dosage of other raw materials is 0~50 parts by weight.
The preparation method of the Semen Cuscutae composite essential oil, includes the following steps：
(1) Semen Cuscutae, the fruit of Chinese wolfberry, raspberry, plantain seed and Schisandra chinensis are crushed respectively, is sieved, obtains Semen Cuscutae powder End, fruit of Chinese wolfberry powder, raspberry powder, Plantago Seed and Chinese Magnolivine Fruit；Each raw material powder is uniformly mixed, is mixed Close object；It when comprising other raw materials, also needs to crush, be sieved, then all raw material powder mixing；
Wherein Semen Cuscutae powder and raspberry powder be middle powder, i.e., No. four sieve, fruit of Chinese wolfberry powder, Plantago Seed and five Taste powder be coarse powder, i.e., No. three sieve；
(2) mixture is placed in supercritical fluid extraction kettle, is 18-30MPa in pressure, temperature is 40~150 DEG C Under the conditions of, it is extracted with Co 2 supercritical fluid, obtains essential oil and residue；
(3) it uses ethanol water that residue is carried out refluxing extraction, obtains extracting solution；Ethanol water described in step (3) The volumetric concentration of solution is 30%~80% (volume of ethyl alcohol is 30~80mL in the solution of 100mL)；Liquid ratio be (8~ 50)：1(mL/g)；The number of the refluxing extraction is 1~3 time, and the time of each refluxing extraction is 0.5~2.5h, refluxing extraction Temperature be 60~90 DEG C；
(4) extracting solution of the essential oil of step (2) and step (3) is mixed using supersonic spraying, obtains Semen Cuscutae Composite essential oil.
Ullrasonic spraying refers to being placed on the essential oil of step (2) and the extracting solution of step (3) is respectively placed in two in step (4) In liquid dispensing apparatus, while two spraying devices of unlatching and ultrasonic transducer, essential oil are uniformly mixed with extracting solution.
The present invention is because essential oil is ethereal oil using ullrasonic spraying mixing, and active material is dissolved in the fat-soluble of ethyl alcohol Substance；Merely blended under agitation is extremely difficult to finely dispersed effect to essential oil with active material；By two kinds of essential oil and active material Liquid mixes, and needs sharp pounding, high acceleration, strong cavitation effect and stirring action using ultrasonic wave, promotes pre- Essential oil and active material the extracting solution quickening being first atomized are mutually dissolved, and are mutually disperseed.The effect of atomization is to increase the mixing of substance Volume, the effect of ultrasonic transducer are to reinforce vibration and cavitation effect.The frequency of the ultrasound is 40~80Hz.
The time extracted described in step (2) is 90~180min, and the flow control of carbon dioxide is in 20~30Kg/h.
The Semen Cuscutae composite essential oil of the present invention is used to prepare skin care item and/or Chinese medicine preparation.
Semen Cuscutae and the fruit of Chinese wolfberry, raspberry, plantain seed, Schisandra chinensis are combined by the present invention so that each efficacy of drugs production Raw synergistic effect dredges profit kidney qi so as to reach life essence-filling, marrow-benefitting, and kind heir spreads out the effect of ancestor.
The main active Flavonoid substances and glucosides substance of Semen Cuscutae, can by increase anterior pituitary, ovary, The development of uterus weight, testis and epididymis promotes estrogen level and Hypothalamus-Pituitary-Ovaries gonad index, inhibits body fluid AsAb, enhancing cellular immunity and antibacterial and anti-inflammation functions, the proliferation and apoptosis for adjusting trophocyte restore normal pregnancy shape State and tocolysis are prevented miscarriage etc. mechanism, play the role of adjusting reproduction active.
The present invention five kinds of medicinal materials complement each other, can reach good treatment impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency rush down, Vaginal bleeding due to deficiency of the kidney during pregnancy and long infertility effect.The composite essential oil of the present invention mainly using the fruit of Chinese wolfberry and the ethereal oil ingredient of Schisandra chinensis as Carrier essential oil, and the flavonoids and polysaccharide active material in five kinds of medicinal materials are as active constituent.Essential oil is mixed with active constituent Afterwards, obtained composite essential oil transdermal characteristic is very good, can quickly be absorbed by the skin into body, reaches preferable curative effect.
In order to preferably play drug effect, present invention preferably employs supercritical liquid extraction technique combination solvent refluxing extraction methods Drug extraction is carried out, composite essential oil is prepared into.Select the fruit of Chinese wolfberry, be because sweet in flavor, mild-natured, tonifying liver through the moon, the sun of kidney-nourishing water； Raspberry, it is sweet to put down into kidney, play a sun and control impotence, controlling nocturnal emission with astringent drugs take the photograph it is excessive, strong kidney and without it is scorching it is partially, controlling nocturnal emission with astringent drugs and undoubtedly puckery evil；Schisandra chinensis is held back Lung nourshing kidney, nourishing generate fluid, sour inducing astrigency, and can arresting seminal emission；Plantain seed cold nature sweet in flavor takes the property of its sharp water tonneau, water to go Then kidney qi is from profit, and water goes fiery benefit to contain, and kidney essense fills Sheng and kidney qi benefit is solid.
Supercritical fluid extraction essential oil of the present invention, solvent refluxing extract active constituent, using ultrasonic spray by essential oil With flavones, polysaccharide isoreactivity substance extracting solution be blended, be not only uniformly mixed, and preserve Semen Cuscutae composite powder in flavonoids, Glycoside, raspberry polysaccharide, polysaccharides isoreactivity substance are not destroyed, and purity is high.Using preceding clean skin, by Semen Cuscutae composite precision Oil massage below the navel skin to absorbing, can improve impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency rush down, vaginal bleeding due to deficiency of the kidney during pregnancy and long not Fertility.Chinese medicine external of the present invention, the various uncomfortable problems for reducing traditional Chinese medicine decocting and taking, promotes the preparation development of Chinese medicine.
Compared with prior art, the invention has the advantages that and advantageous effect：
(1) Semen Cuscutae composite essential oil of the invention is traditional Chinese medicine for outer use, in use, being massaged below human body navel Skin to absorbing, can improve impotence and seminal emission, enuresis frequent micturition, spleen kidney deficiency rush down, vaginal bleeding due to deficiency of the kidney during pregnancy and long infertility；
(2) Semen Cuscutae composite essential oil of the invention is Chinese medicine external, the various uncomfortable problems for reducing traditional Chinese medicine decocting and taking, Promote the preparation development of Chinese medicine；
(3) method that the present invention is extracted by the method and solvent refluxing of supercritical fluid extraction, by the volatilization of medicinal material Oil and active material extract, and are mixed volatile oil and active material by ullrasonic spraying method, and curative effect is more Prominent, toxic side effect is less；
(4) present invention preferably saves the main component of medicinal material in the form of active material extracting solution, and is extracted with medicinal material The essential oil taken preserves original chemical composition of medicinal material and provides the carrier that medicament slow release absorbs, and is provided for modern Chinese herbal medicine preparation One practical effective novel form compatibility program；
(5) present invention extracts having for Chinese medicinal material to greatest extent to the greatest extent using supercritical extract and solvent refluxing extracting method Imitate the volatile oil component in active material and medicinal material, feed intake convenient for preparation, quality control, technology controlling and process, production regularization and Formulation standard ensures drug effect；
(6) present invention mainly extracts essential oil as main carriers from the fruit of Chinese wolfberry and Schisandra chinensis, is mixed with and is with Semen Cuscutae The flavonoids and polysaccharide active material that main various medicinal materials extract, essential oil of the invention have the spy of Thief zone transdermal characteristic Property, promote active drug ingredient more directly, rapidly by genital system tissue under navel to absorb, has ignored extract oral via digestion Various uncomfortable (adverse reactions of the non-principal component or heavy metal of such as medicinal material to liver, intestines, stomach etc.) of system, reach accurate doctor It learns, the purpose of targeted therapy.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to This.No. four sieves, sieve pore internal diameter：250um ± 9.9um, 65 mesh；Coarse powder, No. three sieves, sieve pore internal diameter：355um ± 13um, 50 mesh.No Powder with grain size can more effectively keep pharmaceutically active substance, improve drug effect.
Embodiment 1
A kind of preparation method of Semen Cuscutae composite essential oil, includes the following steps：
(1) by 600 parts by weight of Semen Cuscutae, 400 parts by weight of the fruit of Chinese wolfberry, 150 parts by weight of raspberry, 80 parts by weight of plantain seed, 60 parts by weight of Schisandra chinensis are crushed by high speed disintegrator respectively, and middle powder, matrimony vine is made by No. four sieves respectively in Semen Cuscutae, raspberry Coarse powder is made by No. three sieves in son, plantain seed and Schisandra chinensis, is uniformly mixed so as to obtain composite powder；
(2) composite powder is placed in supercritical fluid extraction kettle, is 20MPa in pressure, under conditions of temperature is 80 DEG C, With co_2 supercritical fluid extraction 120min, flow control obtains essential oil and residue in 22Kg/h, decompression；
(3) residue (can be dried), refluxing extraction is carried out 2 times with the ethanol solution of volume fraction 60%, it is each The condition of secondary refluxing extraction is liquid ratio 20:1 (mL/g), 80 DEG C of water-baths 2 hours, filters to obtain active material extracting solution；
(4) essential oil and active material extracting solution are respectively placed in two liquid dispensing apparatus, while open two sprayings Device and ultrasonic transducer (frequency 60Hz), by liquid filtering in same receiver to get final product Semen Cuscutae composite essential oil.
Embodiment 2
A kind of preparation method of Semen Cuscutae composite essential oil, includes the following steps：
(1) by 500 parts by weight of Semen Cuscutae, 500 parts by weight of the fruit of Chinese wolfberry, 100 parts by weight of raspberry, 60 parts by weight of plantain seed, 80 parts by weight of Schisandra chinensis are crushed by high speed disintegrator respectively, and middle powder, matrimony vine is made by No. four sieves respectively in Semen Cuscutae, raspberry Coarse powder is made by No. three sieves in son, plantain seed and Schisandra chinensis, is uniformly mixed so as to obtain composite powder；
(2) composite powder is placed in supercritical fluid extraction kettle, is 18MPa, the condition that temperature is 150 DEG C in pressure Under, with co_2 supercritical fluid extraction 90min, flow control obtains essential oil and residue in 30Kg/h, decompression；
(3) residue (can be dried), carries out refluxing extraction 2 times with 80% ethyl alcohol of volume fraction, liquid ratio 50:1 (mL/g), 90 DEG C of water-baths 0.5 hour, filter to obtain active material extracting solution；
(4) essential oil and active material extracting solution are respectively placed in two liquid dispensing apparatus, while open two sprayings Device and ultrasonic transducer (frequency 40Hz), by liquid filtering in same receiver to get final product Semen Cuscutae composite essential oil.
Embodiment 3
A kind of preparation method of Semen Cuscutae composite essential oil, includes the following steps：
(1) by 700 parts by weight of Semen Cuscutae, 300 parts by weight of the fruit of Chinese wolfberry, 200 parts by weight of raspberry, 100 parts by weight of plantain seed, 50 parts by weight of Schisandra chinensis are crushed by high speed disintegrator respectively, and middle powder, matrimony vine is made by No. four sieves respectively in Semen Cuscutae, raspberry Coarse powder is made by No. three sieves in son, plantain seed and Schisandra chinensis, is uniformly mixed so as to obtain composite powder；
(2) composite powder is placed in supercritical fluid extraction kettle, is 30MPa in pressure, under conditions of temperature is 40 DEG C, With co_2 supercritical fluid extraction 180min, flow control is depressurized in 20Kg/h, obtains essential oil and residue；
(3) residue (can be dried), carries out refluxing extraction 2 times with 30% ethyl alcohol of volume fraction, liquid ratio 50:1 (mL/g), 60 DEG C of water-baths 2.5 hours, filter to obtain active material extracting solution；
(4) essential oil and active material extracting solution are respectively placed in two liquid dispensing apparatus, while open two sprayings Device and ultrasonic transducer (frequency 80Hz), by liquid filtering in same receiver to get final product Semen Cuscutae composite essential oil.
Performance test：
Skin permeation rate is tested：
The preparation of isolated skin：The hair that mouse web portion is sloughed with 8%Na2S solution, for 24 hours afterwards puts to death mouse, cuts skin Skin, it is careful to reject subcutaneous fat and be adhered tissue, it is rinsed well repeatedly with physiological saline, is cut into size appropriate, sets physiology salt It is spare in 4 DEG C of preservations in water.
Transdermal experiment：The skin handled well is fixed between the supply pool and reception tank of the Franz diffusion cells of improvement, very Skin side is contacted with receiving liquid, empties bubble, and stratum corneum side is to supply pool.By 2ml essential oils drop on mouse skin, receiving medium is - 0.1% phosphoric acid solution (17 of nitrile:83).Magnetic agitation speed is about 250r/min, and bath temperature is 37 DEG C.Respectively at 2,4,8, 12, for 24 hours, it is taken out from reception tank and receives medium 2mL, while adding the fresh reception medium of equivalent into reception tank.It takes out Medium is received after 0.45 μm of miillpore filter filters, sample introduction is analyzed for HPLC.
Release test：It is handled by HPLC method data measureds, unit area accumulates infiltration capacity Qn=(the total Cn+ Σ Cn- of V 1V takes)/A, Cn is the drug quality concentration (μ g/mL) that n-th of sample point measures, and Cn-1 is what (n-1)th sample point measured Drug quality concentration (μ g/mL), A are infiltrating area (cm2), and V is always the volume (mL) of diffusion cell receiving chamber, and V is taken as sampling amount (mL), Σ Cn-1V be taken as sampling lost dose accumulation and, specific testing result is as shown in table 1：
Semen Cuscutae composite essential oil Transdermal absorption prepared by 1 Examples 1 to 3 of table tests effect
Detection content Embodiment 1 Embodiment 2 Embodiment 3
Unit area accumulates infiltration capacity (μ g/cm2) 160 132 175
As it can be seen that the Transdermal absorption effect of Semen Cuscutae compound essential oil proposed by the present invention is very good.
Curative effect is tested：
SD female rats 40, weight 180-220g are taken to be randomly divided into 4 groups：Normal group, 1 group of embodiment, embodiment 2 groups, 3 groups of embodiment.Adapt to 2 days, check that vaginal smear observes the oestrous cycle daily later, selection continuously have 2 it is normal emotionally For modeling, remaining is eliminated the rat in period.In addition to Normal group, each group drips the embodiment essential oil of a 5ml daily respectively Below rat abdomen, model 20 days.Rat hippocampus, hypothalamus, hypophysis and ovary, 10% formaldehyde is taken to fix after modeling rapidly, Paraffin embedding does pathological section, carries out immunohistochemical assay, detection estrogen receptor (ER) and ovary Follicle Stimulating Hormone Receptors (FSHR), promote corpus luteum (generation) hormone receptor (LHR), as a result such as table 2.
The effect of Semen Cuscutae composite essential oil of 2 Examples 1 to 3 of table test result (μ g/ml)
So as it can be seen that the zoopery curative effect of Semen Cuscutae compound essential oil proposed by the present invention is very good.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by the embodiment Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications, Equivalent substitute mode is should be, is included within the scope of the present invention.

Claims (10)

1. a kind of Semen Cuscutae composite essential oil, it is characterised in that：Mainly extract to obtain by the following raw material counted in parts by weight：Tu 500-700 parts sub-, 300-500 parts of the fruit of Chinese wolfberry, 100-200 parts of raspberry, 60-100 parts of plantain seed, 50-80 parts of Schisandra chinensis；
The extraction refers to then using ethanol water that will extract essential oil using essential oil in supercritical fluid extraction above-mentioned raw materials Residue afterwards carries out refluxing extraction, and the extracting solution of refluxing extraction is mixed with essential oil.
2. Semen Cuscutae composite essential oil according to claim 1, it is characterised in that：Original selected by the Semen Cuscutae composite essential oil Material further includes more than one in emblic, the fruit of glossy privet and the fruit of Cherokee rose.
3. according to the preparation method of any one of the claim 1~2 Semen Cuscutae composite essential oil, it is characterised in that：Including following Step：
(1) Semen Cuscutae, the fruit of Chinese wolfberry, raspberry, plantain seed and Schisandra chinensis are crushed respectively, is sieved, obtains Semen Cuscutae powder, Chinese holly Matrimony vine powder, raspberry powder, Plantago Seed and Chinese Magnolivine Fruit；Each raw material powder is uniformly mixed, mixture is obtained； It when comprising other raw materials, also needs to crush, be sieved, then all raw material powder mixing；
(2) mixture is placed in supercritical fluid extraction kettle, is 18~30MPa, the condition that temperature is 40~150 DEG C in pressure Under, it is extracted with Co 2 supercritical fluid, obtains essential oil and residue；
(3) it uses ethanol water that residue is carried out refluxing extraction, obtains extracting solution；
(4) extracting solution of the essential oil of step (2) and step (3) is mixed using supersonic spraying, it is compound obtains Semen Cuscutae Essential oil.
4. the preparation method of Semen Cuscutae composite essential oil according to claim 3, it is characterised in that：Step extracts described in (2) Time be 90~180min, the flow control of carbon dioxide is in 20~30Kg/h.
5. the preparation method of Semen Cuscutae composite essential oil according to claim 3, it is characterised in that：Ethyl alcohol described in step (3) The volumetric concentration of aqueous solution is 30%~80%.
6. the preparation method of Semen Cuscutae composite essential oil according to claim 3, it is characterised in that：Refluxing extraction in step (3) When liquid ratio be (8~50) mL：1g；The number of the refluxing extraction is 1~3 time, time of each refluxing extraction is 0.5~ The temperature of 2.5h, refluxing extraction are 60~90 DEG C.
7. the preparation method of Semen Cuscutae composite essential oil according to claim 3, it is characterised in that：Ullrasonic spraying in step (4) Refer to the extracting solution of the essential oil of step (2) and step (3) being respectively placed in two liquid dispensing apparatus, while opening two sprays Mist device and ultrasonic transducer, essential oil are uniformly mixed with extracting solution.
8. the preparation method of Semen Cuscutae composite essential oil according to claim 7, it is characterised in that：The frequency of the ultrasound is 40-80Hz。
9. the preparation method of Semen Cuscutae composite essential oil according to claim 3, it is characterised in that：Semen Cuscutae powder and raspberry Powder be middle powder, i.e., No. four sieve, fruit of Chinese wolfberry powder, Plantago Seed and Chinese Magnolivine Fruit be coarse powder, i.e., No. three sieve.
10. according to the application of any one of the claim 1~2 Semen Cuscutae composite essential oil, it is characterised in that：The Semen Cuscutae is multiple It closes essential oil and is used to prepare skin care item and/or Chinese medicine preparation.
CN201810508882.0A 2018-05-24 2018-05-24 A kind of Semen Cuscutae composite essential oil and the preparation method and application thereof Pending CN108721435A (en)

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